Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07671.t1 | KEH38942.1 | 65.8 | 114 | 38 | 1 | 1 | 113 | 1 | 114 | 1.70E-27 | 132.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07671.t1 | Q8LJS2 | 49.1 | 114 | 54 | 1 | 1 | 114 | 1 | 110 | 2.9e-20 | 100.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07671.t1 | A0A072VB01 | 65.8 | 114 | 38 | 1 | 1 | 113 | 1 | 114 | 1.2e-27 | 132.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene07671.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07671.t1 | MTR_2g084815 | 75.824 | 91 | 22 | 0 | 1 | 91 | 1 | 91 | 2.71e-42 | 144 |
| MS.gene07671.t1 | MTR_2g084815 | 64.286 | 56 | 16 | 2 | 89 | 140 | 221 | 276 | 1.91e-13 | 67.4 |
| MS.gene07671.t1 | MTR_2g084815 | 57.252 | 131 | 52 | 1 | 1 | 131 | 1 | 127 | 1.11e-41 | 141 |
| MS.gene07671.t1 | MTR_2g084815 | 57.252 | 131 | 52 | 1 | 1 | 131 | 1 | 127 | 4.58e-41 | 139 |
| MS.gene07671.t1 | MTR_2g084815 | 57.252 | 131 | 52 | 1 | 1 | 131 | 1 | 127 | 5.71e-41 | 140 |
| MS.gene07671.t1 | MTR_2g084815 | 61.017 | 59 | 19 | 2 | 89 | 143 | 244 | 302 | 2.37e-13 | 67.4 |
| MS.gene07671.t1 | MTR_4g055440 | 50.000 | 98 | 49 | 0 | 1 | 98 | 5 | 102 | 5.90e-26 | 100 |
| MS.gene07671.t1 | MTR_4g055450 | 44.330 | 97 | 42 | 1 | 2 | 98 | 6 | 90 | 2.86e-17 | 79.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07671.t1 | AT5G03740 | 41.860 | 86 | 47 | 2 | 1 | 86 | 1 | 83 | 2.45e-16 | 75.9 |
| MS.gene07671.t1 | AT5G22650 | 42.697 | 89 | 47 | 2 | 1 | 89 | 1 | 85 | 1.46e-15 | 73.6 |
| MS.gene07671.t1 | AT5G22650 | 42.697 | 89 | 47 | 2 | 1 | 89 | 1 | 85 | 1.54e-15 | 73.6 |
| MS.gene07671.t1 | AT3G44750 | 37.736 | 106 | 53 | 3 | 1 | 96 | 1 | 103 | 5.80e-13 | 65.9 |
| MS.gene07671.t1 | AT3G44750 | 37.736 | 106 | 53 | 3 | 1 | 96 | 1 | 103 | 6.00e-13 | 65.9 |
Find 47 sgRNAs with CRISPR-Local
Find 81 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACCGTTTCCAATGGAGTTT+TGG | 0.171376 | 4.1:-42500703 | None:intergenic |
| GTGGTTATTTACCTCGTATT+TGG | 0.275316 | 4.1:-42500228 | MS.gene07671:CDS |
| GAAATAAACACTCGCAGTTT+TGG | 0.276973 | 4.1:+42500107 | None:intergenic |
| AGGACATTCACCTCTGAAAA+TGG | 0.279756 | 4.1:-42499056 | MS.gene07671:CDS |
| AGGATATTCACCTCTGAAAA+TGG | 0.283123 | 4.1:-42499229 | MS.gene07671:CDS |
| AGGATATTCACCTCTGAAAA+TGG | 0.283123 | 4.1:-42499402 | MS.gene07671:CDS |
| TAAATAACCACAGGCTCTTC+TGG | 0.321440 | 4.1:+42500240 | None:intergenic |
| GAAGTTTCAGCATCATTGTT+AGG | 0.324985 | 4.1:+42499548 | None:intergenic |
| TGGTGGCCAGAAGATTGTTT+TGG | 0.336064 | 4.1:-42500208 | MS.gene07671:CDS |
| TGAATTAGCTGCCGAGGTTA+AGG | 0.369389 | 4.1:-42500460 | MS.gene07671:intron |
| AGGTTCCCAAAACAATCTTC+TGG | 0.382371 | 4.1:+42500202 | None:intergenic |
| GCACTTGGTGCAGCAAATAA+AGG | 0.387943 | 4.1:-42500267 | MS.gene07671:CDS |
| TTAGGACCTTTCTTAGAGTC+AGG | 0.425080 | 4.1:+42499566 | None:intergenic |
| GATGCTGAAACTTCCAAGTC+TGG | 0.441612 | 4.1:-42499539 | MS.gene07671:CDS |
| AAGCATACCTGCTGCAACTA+CGG | 0.449498 | 4.1:+42499504 | None:intergenic |
| AGCAGCTATTCACATTTCTC+AGG | 0.458967 | 4.1:-42500397 | MS.gene07671:intron |
| TTGTTAGATTCTGAGAAAGC+TGG | 0.482257 | 4.1:-42499602 | MS.gene07671:intron |
| GCAAGAGAGTCCTCCAGACT+TGG | 0.490554 | 4.1:+42499526 | None:intergenic |
| ACTTCACTTAGCAGCATGCT+TGG | 0.492494 | 4.1:+42499013 | None:intergenic |
| GTTATTTACCTCGTATTTGG+TGG | 0.494314 | 4.1:-42500225 | MS.gene07671:CDS |
| TGGATTGCAACAACATATGA+AGG | 0.511482 | 4.1:-42499209 | MS.gene07671:CDS |
| TGGATTGCAACAACATATGA+AGG | 0.511482 | 4.1:-42499382 | MS.gene07671:CDS |
| TCTCTTGCCGTAGTTGCAGC+AGG | 0.513623 | 4.1:-42499511 | MS.gene07671:intron |
| GCTGCTGCATCATGGGCATA+CGG | 0.519271 | 4.1:+42500414 | None:intergenic |
| GCAATTCCTGACTCTAAGAA+AGG | 0.524303 | 4.1:-42499572 | MS.gene07671:CDS |
| CTGCTGCATCATGGGCATAC+GGG | 0.528977 | 4.1:+42500415 | None:intergenic |
| GAGTCCTCTAGGAGCATGCT+TGG | 0.529371 | 4.1:+42499186 | None:intergenic |
| GAGTCCTCTAGGAGCATGCT+TGG | 0.529371 | 4.1:+42499359 | None:intergenic |
| CTTCTGCAACAGAGTCCTCT+AGG | 0.537070 | 4.1:+42499175 | None:intergenic |
| CTTCTGCAACAGAGTCCTCT+AGG | 0.537070 | 4.1:+42499348 | None:intergenic |
| GTGAATAGCTGCTGCATCAT+GGG | 0.548035 | 4.1:+42500407 | None:intergenic |
| GTTATTGTATAGGTAGCACT+TGG | 0.550528 | 4.1:-42500282 | MS.gene07671:intron |
| TTAGCTGCCGAGGTTAAGGT+TGG | 0.554128 | 4.1:-42500456 | MS.gene07671:intron |
| TGTGAATAGCTGCTGCATCA+TGG | 0.561080 | 4.1:+42500406 | None:intergenic |
| AGGTTACCCCAAAACTCCAT+TGG | 0.577427 | 4.1:+42500695 | None:intergenic |
| AGTTTCTCCAACCTTAACCT+CGG | 0.624909 | 4.1:+42500449 | None:intergenic |
| GATCTTTGAATTAGCTGCCG+AGG | 0.634911 | 4.1:-42500466 | MS.gene07671:intron |
| TGGATTGCAAGAACATAACA+AGG | 0.641394 | 4.1:-42499036 | MS.gene07671:CDS |
| AATACGAGGTAAATAACCAC+AGG | 0.651072 | 4.1:+42500231 | None:intergenic |
| ATATTATTAAAAGTTATCTG+AGG | 0.654039 | 4.1:+42500162 | None:intergenic |
| AAGGCCAAGCATGCTCCTAG+AGG | 0.658563 | 4.1:-42499190 | MS.gene07671:CDS |
| AAGGCCAAGCATGCTCCTAG+AGG | 0.658563 | 4.1:-42499363 | MS.gene07671:CDS |
| GCTGAAACTTCCAAGTCTGG+AGG | 0.660898 | 4.1:-42499536 | MS.gene07671:CDS |
| TCTGTTGCAGAAGTTGCAAG+AGG | 0.682206 | 4.1:-42499165 | MS.gene07671:intron |
| TCTGTTGCAGAAGTTGCAAG+AGG | 0.682206 | 4.1:-42499338 | MS.gene07671:intron |
| TCTTCTGGCCACCAAATACG+AGG | 0.688955 | 4.1:+42500217 | None:intergenic |
| AGGCATACCAGAAGAGCCTG+TGG | 0.689406 | 4.1:-42500247 | MS.gene07671:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATATTTATTACTTTTAGA+TGG | - | chr4.1:42500244-42500263 | MS.gene07671:CDS | 10.0% |
| !!! | TAATAGTTTAAATTTTACAT+TGG | - | chr4.1:42499901-42499920 | MS.gene07671:intron | 10.0% |
| !!! | TATATTTATTACTTTTAGAT+GGG | - | chr4.1:42500245-42500264 | MS.gene07671:CDS | 10.0% |
| !!! | TTTTAATAATATTCTTGAAA+AGG | - | chr4.1:42499557-42499576 | MS.gene07671:CDS | 10.0% |
| !! | AAAAATTTAAGGACAAATTA+CGG | + | chr4.1:42500088-42500107 | None:intergenic | 15.0% |
| !! | AAAGTAATAAATATATGCTT+CGG | + | chr4.1:42500240-42500259 | None:intergenic | 15.0% |
| !! | AAAGTAATAAATATATGCTT+TGG | + | chr4.1:42500240-42500259 | None:intergenic | 15.0% |
| !! | AAGTAATAAATATATGCTTT+GGG | + | chr4.1:42500239-42500258 | None:intergenic | 15.0% |
| !! | ATATTATTAAAAGTTATCTG+AGG | + | chr4.1:42499549-42499568 | None:intergenic | 15.0% |
| !! | AAGTAATAAATATATGCTTC+GGG | + | chr4.1:42500412-42500431 | None:intergenic | 20.0% |
| !! | CAGAATCTAACAAAAATTTA+AGG | + | chr4.1:42500099-42500118 | None:intergenic | 20.0% |
| !! | TAAAGACAAGAAAAAAAGAA+AGG | + | chr4.1:42500281-42500300 | None:intergenic | 20.0% |
| !! | TAATAAATAAGTCTCAACTA+AGG | + | chr4.1:42499654-42499673 | None:intergenic | 20.0% |
| !! | TAATAGCTTAACATGTATAT+TGG | - | chr4.1:42499752-42499771 | MS.gene07671:intron | 20.0% |
| ! | GAAGAAAATTAGATGATGAA+AGG | + | chr4.1:42499048-42499067 | None:intergenic | 25.0% |
| !! | TCTTAACTATTGATGTGTTT+TGG | - | chr4.1:42499940-42499959 | MS.gene07671:intron | 25.0% |
| !! | TTTTTCTTGTCTTTATTACC+AGG | - | chr4.1:42500286-42500305 | MS.gene07671:intron | 25.0% |
| AACAGATCTATAAGACACTT+AGG | + | chr4.1:42499858-42499877 | None:intergenic | 30.0% | |
| ATGATGAAAGGAGAAATATG+AGG | + | chr4.1:42499036-42499055 | None:intergenic | 30.0% | |
| ATGTCAATGCTGAAATATTG+AGG | - | chr4.1:42500019-42500038 | MS.gene07671:intron | 30.0% | |
| ATGTGTAACAGAAGGATATA+TGG | + | chr4.1:42500065-42500084 | None:intergenic | 30.0% | |
| TAAGCTATTATTGCAACACT+AGG | + | chr4.1:42499742-42499761 | None:intergenic | 30.0% | |
| TATTCACATTTCTCAGGTTA+GGG | - | chr4.1:42499317-42499336 | MS.gene07671:intron | 30.0% | |
| AATACGAGGTAAATAACCAC+AGG | + | chr4.1:42499480-42499499 | None:intergenic | 35.0% | |
| AATTACGGATGTGTAACAGA+AGG | + | chr4.1:42500073-42500092 | None:intergenic | 35.0% | |
| CTATTCACATTTCTCAGGTT+AGG | - | chr4.1:42499316-42499335 | MS.gene07671:intron | 35.0% | |
| GAAGTTTCAGCATCATTGTT+AGG | + | chr4.1:42500163-42500182 | None:intergenic | 35.0% | |
| GTGGTTATTTACCTCGTATT+TGG | - | chr4.1:42499480-42499499 | MS.gene07671:intron | 35.0% | |
| GTTATTGTATAGGTAGCACT+TGG | - | chr4.1:42499426-42499445 | MS.gene07671:intron | 35.0% | |
| GTTATTTACCTCGTATTTGG+TGG | - | chr4.1:42499483-42499502 | MS.gene07671:intron | 35.0% | |
| TGGATTGCAACAACATATGA+AGG | - | chr4.1:42500326-42500345 | MS.gene07671:intron | 35.0% | |
| TGGATTGCAAGAACATAACA+AGG | - | chr4.1:42500672-42500691 | MS.gene07671:intron | 35.0% | |
| ! | AGGAATTTTGTCCCTGATTA+AGG | + | chr4.1:42499529-42499548 | None:intergenic | 35.0% |
| ! | AGGATATTCACCTCTGAAAA+TGG | - | chr4.1:42500306-42500325 | MS.gene07671:intron | 35.0% |
| ! | ATTTTCAGAGGTGAATATCC+TGG | + | chr4.1:42500307-42500326 | None:intergenic | 35.0% |
| ! | GAAATAAACACTCGCAGTTT+TGG | + | chr4.1:42499604-42499623 | None:intergenic | 35.0% |
| ! | GGTTTACTAAATTGCTGCTT+TGG | - | chr4.1:42499404-42499423 | MS.gene07671:intron | 35.0% |
| ! | TTCTTGCAATCCATTTTCAG+AGG | + | chr4.1:42500665-42500684 | None:intergenic | 35.0% |
| ! | TTGTTAGATTCTGAGAAAGC+TGG | - | chr4.1:42500106-42500125 | MS.gene07671:intron | 35.0% |
| ! | TTGTTGCAATCCATTTTCAG+AGG | + | chr4.1:42500319-42500338 | None:intergenic | 35.0% |
| !! | ACGTTGTGTGTTTTTGTCTA+GGG | - | chr4.1:42499383-42499402 | MS.gene07671:CDS | 35.0% |
| !! | TACGTTGTGTGTTTTTGTCT+AGG | - | chr4.1:42499382-42499401 | MS.gene07671:CDS | 35.0% |
| !! | TGCTGCTTTGGTTATTGTAT+AGG | - | chr4.1:42499416-42499435 | MS.gene07671:intron | 35.0% |
| !!! | TGTTTTGGGAACCTTAATCA+GGG | - | chr4.1:42499515-42499534 | MS.gene07671:CDS | 35.0% |
| !!! | TTGTTTTGGGAACCTTAATC+AGG | - | chr4.1:42499514-42499533 | MS.gene07671:CDS | 35.0% |
| ACTGCGAGTGTTTATTTCAG+TGG | - | chr4.1:42499606-42499625 | MS.gene07671:intron | 40.0% | |
| AGCAGCTATTCACATTTCTC+AGG | - | chr4.1:42499311-42499330 | MS.gene07671:intron | 40.0% | |
| AGGTTCCCAAAACAATCTTC+TGG | + | chr4.1:42499509-42499528 | None:intergenic | 40.0% | |
| AGTTTCTCCAACCTTAACCT+CGG | + | chr4.1:42499262-42499281 | None:intergenic | 40.0% | |
| TAAATAACCACAGGCTCTTC+TGG | + | chr4.1:42499471-42499490 | None:intergenic | 40.0% | |
| TGTACGTACGTACGTGTATT+AGG | - | chr4.1:42499190-42499209 | MS.gene07671:CDS | 40.0% | |
| TTAGGACCTTTCTTAGAGTC+AGG | + | chr4.1:42500145-42500164 | None:intergenic | 40.0% | |
| ! | AGGACATTCACCTCTGAAAA+TGG | - | chr4.1:42500652-42500671 | MS.gene07671:intron | 40.0% |
| ! | ATTTTCAGAGGTGAATGTCC+TGG | + | chr4.1:42500653-42500672 | None:intergenic | 40.0% |
| ! | GCAATTCCTGACTCTAAGAA+AGG | - | chr4.1:42500136-42500155 | MS.gene07671:CDS | 40.0% |
| AAGCATACCTGCTGCAACTA+CGG | + | chr4.1:42500207-42500226 | None:intergenic | 45.0% | |
| AGTGAGGAAGAAGGTGAAAC+TGG | - | chr4.1:42499971-42499990 | MS.gene07671:intron | 45.0% | |
| GATCTTTGAATTAGCTGCCG+AGG | - | chr4.1:42499242-42499261 | MS.gene07671:intron | 45.0% | |
| GATGCTGAAACTTCCAAGTC+TGG | - | chr4.1:42500169-42500188 | MS.gene07671:CDS | 45.0% | |
| GTGAATAGCTGCTGCATCAT+GGG | + | chr4.1:42499304-42499323 | None:intergenic | 45.0% | |
| TCTGTTGCAGAAGTTGCAAG+AGG | - | chr4.1:42500370-42500389 | MS.gene07671:intron | 45.0% | |
| TGAATTAGCTGCCGAGGTTA+AGG | - | chr4.1:42499248-42499267 | MS.gene07671:intron | 45.0% | |
| TGTGAATAGCTGCTGCATCA+TGG | + | chr4.1:42499305-42499324 | None:intergenic | 45.0% | |
| ! | GGTGGCCAGAAGATTGTTTT+GGG | - | chr4.1:42499501-42499520 | MS.gene07671:intron | 45.0% |
| ! | TGGTGGCCAGAAGATTGTTT+TGG | - | chr4.1:42499500-42499519 | MS.gene07671:intron | 45.0% |
| !! | GCACTTGGTGCAGCAAATAA+AGG | - | chr4.1:42499441-42499460 | MS.gene07671:intron | 45.0% |
| CTTCTGCAACAGAGTCCTCT+AGG | + | chr4.1:42500363-42500382 | None:intergenic | 50.0% | |
| GCTGAAACTTCCAAGTCTGG+AGG | - | chr4.1:42500172-42500191 | MS.gene07671:CDS | 50.0% | |
| GGAAGAAGGTGAAACTGGTG+AGG | - | chr4.1:42499976-42499995 | MS.gene07671:intron | 50.0% | |
| TCTTCTGGCCACCAAATACG+AGG | + | chr4.1:42499494-42499513 | None:intergenic | 50.0% | |
| TTAGCTGCCGAGGTTAAGGT+TGG | - | chr4.1:42499252-42499271 | MS.gene07671:intron | 50.0% | |
| AAGGCCAAGCATGCTCCTAG+AGG | - | chr4.1:42500345-42500364 | MS.gene07671:intron | 55.0% | |
| ACCTTCTTCCTCACTCGTGC+AGG | + | chr4.1:42499966-42499985 | None:intergenic | 55.0% | |
| AGGCATACCAGAAGAGCCTG+TGG | - | chr4.1:42499461-42499480 | MS.gene07671:intron | 55.0% | |
| CTGCTGCATCATGGGCATAC+GGG | + | chr4.1:42499296-42499315 | None:intergenic | 55.0% | |
| GCAAGAGAGTCCTCCAGACT+TGG | + | chr4.1:42500185-42500204 | None:intergenic | 55.0% | |
| GCTGCTGCATCATGGGCATA+CGG | + | chr4.1:42499297-42499316 | None:intergenic | 55.0% | |
| TCTCTTGCCGTAGTTGCAGC+AGG | - | chr4.1:42500197-42500216 | MS.gene07671:CDS | 55.0% | |
| ! | GAGTCCTCTAGGAGCATGCT+TGG | + | chr4.1:42500352-42500371 | None:intergenic | 55.0% |
| !!! | TGTTTTGGCCTGCACGAGTG+AGG | - | chr4.1:42499955-42499974 | MS.gene07671:intron | 55.0% |
| GCCTGCACGAGTGAGGAAGA+AGG | - | chr4.1:42499962-42499981 | MS.gene07671:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 42499016 | 42500714 | 42499016 | ID=MS.gene07671 |
| chr4.1 | mRNA | 42499016 | 42500714 | 42499016 | ID=MS.gene07671.t1;Parent=MS.gene07671 |
| chr4.1 | exon | 42500702 | 42500714 | 42500702 | ID=MS.gene07671.t1.exon1;Parent=MS.gene07671.t1 |
| chr4.1 | CDS | 42500702 | 42500714 | 42500702 | ID=cds.MS.gene07671.t1;Parent=MS.gene07671.t1 |
| chr4.1 | exon | 42500398 | 42500474 | 42500398 | ID=MS.gene07671.t1.exon2;Parent=MS.gene07671.t1 |
| chr4.1 | CDS | 42500398 | 42500474 | 42500398 | ID=cds.MS.gene07671.t1;Parent=MS.gene07671.t1 |
| chr4.1 | exon | 42500118 | 42500292 | 42500118 | ID=MS.gene07671.t1.exon3;Parent=MS.gene07671.t1 |
| chr4.1 | CDS | 42500118 | 42500292 | 42500118 | ID=cds.MS.gene07671.t1;Parent=MS.gene07671.t1 |
| chr4.1 | exon | 42499512 | 42499617 | 42499512 | ID=MS.gene07671.t1.exon4;Parent=MS.gene07671.t1 |
| chr4.1 | CDS | 42499512 | 42499617 | 42499512 | ID=cds.MS.gene07671.t1;Parent=MS.gene07671.t1 |
| chr4.1 | exon | 42499339 | 42499422 | 42499339 | ID=MS.gene07671.t1.exon5;Parent=MS.gene07671.t1 |
| chr4.1 | CDS | 42499339 | 42499422 | 42499339 | ID=cds.MS.gene07671.t1;Parent=MS.gene07671.t1 |
| chr4.1 | exon | 42499166 | 42499249 | 42499166 | ID=MS.gene07671.t1.exon6;Parent=MS.gene07671.t1 |
| chr4.1 | CDS | 42499166 | 42499249 | 42499166 | ID=cds.MS.gene07671.t1;Parent=MS.gene07671.t1 |
| chr4.1 | exon | 42499016 | 42499076 | 42499016 | ID=MS.gene07671.t1.exon7;Parent=MS.gene07671.t1 |
| chr4.1 | CDS | 42499016 | 42499076 | 42499016 | ID=cds.MS.gene07671.t1;Parent=MS.gene07671.t1 |
| Gene Sequence |
| Protein sequence |
