Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07804.t1 | AET01618.1 | 78 | 264 | 40 | 3 | 1 | 264 | 1 | 246 | 2.90E-101 | 378.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07804.t1 | Q8LDF9 | 39.9 | 153 | 79 | 4 | 147 | 290 | 32 | 180 | 2.7e-15 | 84.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07804.t1 | G7LI68 | 78.0 | 264 | 40 | 3 | 1 | 264 | 1 | 246 | 2.1e-101 | 378.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene07804.t1 | TR | HMG |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07804.t1 | MTR_8g018100 | 81.818 | 264 | 30 | 3 | 1 | 264 | 1 | 246 | 4.26e-150 | 422 |
| MS.gene07804.t1 | MTR_1g115220 | 73.057 | 193 | 37 | 3 | 147 | 329 | 23 | 210 | 1.55e-78 | 239 |
| MS.gene07804.t1 | MTR_3g115580 | 47.191 | 89 | 47 | 0 | 211 | 299 | 32 | 120 | 1.56e-18 | 81.6 |
| MS.gene07804.t1 | MTR_5g024180 | 41.905 | 105 | 57 | 1 | 208 | 308 | 16 | 120 | 1.15e-16 | 75.9 |
| MS.gene07804.t1 | MTR_7g068280 | 44.595 | 74 | 41 | 0 | 220 | 293 | 47 | 120 | 5.24e-14 | 69.3 |
| MS.gene07804.t1 | MTR_8g073345 | 33.636 | 110 | 67 | 2 | 208 | 312 | 16 | 124 | 1.00e-12 | 65.1 |
| MS.gene07804.t1 | MTR_8g073345 | 33.636 | 110 | 67 | 2 | 208 | 312 | 16 | 124 | 1.03e-12 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07804.t1 | AT5G23420 | 46.053 | 152 | 69 | 4 | 147 | 289 | 32 | 179 | 4.68e-25 | 101 |
| MS.gene07804.t1 | AT4G35570 | 45.570 | 79 | 43 | 0 | 215 | 293 | 25 | 103 | 2.85e-16 | 74.7 |
| MS.gene07804.t1 | AT4G35570 | 45.570 | 79 | 43 | 0 | 215 | 293 | 25 | 103 | 2.85e-16 | 74.7 |
| MS.gene07804.t1 | AT3G51880 | 38.393 | 112 | 68 | 1 | 205 | 316 | 35 | 145 | 1.49e-15 | 74.3 |
| MS.gene07804.t1 | AT3G51880 | 38.393 | 112 | 68 | 1 | 205 | 316 | 35 | 145 | 2.42e-15 | 73.6 |
| MS.gene07804.t1 | AT3G51880 | 38.393 | 112 | 68 | 1 | 205 | 316 | 35 | 145 | 2.42e-15 | 73.6 |
| MS.gene07804.t1 | AT3G51880 | 38.393 | 112 | 68 | 1 | 205 | 316 | 35 | 145 | 2.42e-15 | 73.6 |
| MS.gene07804.t1 | AT3G51880 | 38.462 | 104 | 63 | 1 | 204 | 307 | 34 | 136 | 5.26e-15 | 72.0 |
| MS.gene07804.t1 | AT2G17560 | 38.710 | 93 | 56 | 1 | 215 | 306 | 25 | 117 | 3.04e-13 | 66.6 |
| MS.gene07804.t1 | AT2G17560 | 38.710 | 93 | 56 | 1 | 215 | 306 | 25 | 117 | 3.46e-13 | 66.2 |
| MS.gene07804.t1 | AT2G17560 | 38.710 | 93 | 56 | 1 | 215 | 306 | 25 | 117 | 3.46e-13 | 66.2 |
| MS.gene07804.t1 | AT1G20693 | 40.816 | 98 | 56 | 2 | 212 | 307 | 25 | 122 | 9.35e-13 | 65.5 |
| MS.gene07804.t1 | AT1G20693 | 40.816 | 98 | 56 | 2 | 212 | 307 | 25 | 122 | 9.56e-13 | 65.5 |
| MS.gene07804.t1 | AT1G20693 | 40.816 | 98 | 56 | 2 | 212 | 307 | 25 | 122 | 1.13e-12 | 65.1 |
| MS.gene07804.t1 | AT1G20696 | 40.777 | 103 | 59 | 2 | 206 | 307 | 18 | 119 | 1.30e-12 | 64.7 |
| MS.gene07804.t1 | AT1G20696 | 40.217 | 92 | 54 | 1 | 206 | 297 | 18 | 108 | 9.54e-12 | 62.4 |
| MS.gene07804.t1 | AT1G20696 | 40.217 | 92 | 54 | 1 | 206 | 297 | 18 | 108 | 4.85e-11 | 60.5 |
Find 64 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATATTCACAAACTAAAAT+TGG | 0.146005 | 8.3:+72463411 | None:intergenic |
| GGCGAAAGCACTGCCATCTT+TGG | 0.263194 | 8.3:+72463789 | None:intergenic |
| CGAGTTGAATCAAAGCTTCT+TGG | 0.289462 | 8.3:+72463821 | None:intergenic |
| AAGCCTCCTGCTGCTGTTCA+AGG | 0.291500 | 8.3:+72463968 | None:intergenic |
| TTGCAGACGAGGAGGAGATC+AGG | 0.340582 | 8.3:-72464178 | MS.gene07804:CDS |
| AATATTCACAAACTAAAATT+GGG | 0.355318 | 8.3:+72463412 | None:intergenic |
| TAAAGTTGGGAAAGGGAAAA+AGG | 0.356050 | 8.3:-72463299 | MS.gene07804:CDS |
| TGTTGACCTTGTTTGCTTCA+AGG | 0.365012 | 8.3:+72464258 | None:intergenic |
| TGTGATGCGCATCATGTTGA+TGG | 0.383923 | 8.3:-72463889 | MS.gene07804:CDS |
| TCTTCAGGATCAGCGTTAAT+CGG | 0.386915 | 8.3:+72464233 | None:intergenic |
| AATCAAAGCTTCTTGGGTAA+TGG | 0.393707 | 8.3:+72463828 | None:intergenic |
| TCTTCCGCCTCTCCTGGTGC+AGG | 0.396540 | 8.3:+72447702 | None:intergenic |
| AGCCTCCTGCTGCTGTTCAA+GGG | 0.417728 | 8.3:+72463969 | None:intergenic |
| TTGATTCAAAGGATGTTAAG+AGG | 0.429062 | 8.3:-72463103 | MS.gene07804:CDS |
| GTTAACTCTTCCGCCTCTCC+TGG | 0.437006 | 8.3:+72447696 | None:intergenic |
| CTTCCGCCTCTCCTGGTGCA+GGG | 0.453369 | 8.3:+72447703 | None:intergenic |
| GCCTCCTGCTGCTGTTCAAG+GGG | 0.455833 | 8.3:+72463970 | None:intergenic |
| ATGGCAGTGCTTTCGCCAAA+TGG | 0.458037 | 8.3:-72463783 | MS.gene07804:intron |
| ACTTTAGTCCGTTTCGCAGT+TGG | 0.458641 | 8.3:+72463316 | None:intergenic |
| GGAAGGCCAATCTGATGATA+AGG | 0.467371 | 8.3:-72447730 | MS.gene07804:CDS |
| GGAAGCTAATCTTGATTCAA+AGG | 0.468919 | 8.3:-72463114 | MS.gene07804:CDS |
| TCACAAATCTTCTTTGAAGC+AGG | 0.469888 | 8.3:+72463907 | None:intergenic |
| ACTGCGAAACGGACTAAAGT+TGG | 0.477591 | 8.3:-72463313 | MS.gene07804:CDS |
| GGGCTTCCTTATCATCAGAT+TGG | 0.481597 | 8.3:+72447724 | None:intergenic |
| AGCCCCTGCACCAGGAGAGG+CGG | 0.489136 | 8.3:-72447706 | MS.gene07804:CDS |
| CTCGCAAAATGTGCCAAAGA+TGG | 0.495720 | 8.3:-72463802 | MS.gene07804:CDS |
| GCCAAAATCAAAATGAATCT+CGG | 0.501868 | 8.3:-72463599 | MS.gene07804:intron |
| GAGTTGAATCAAAGCTTCTT+GGG | 0.504888 | 8.3:+72463822 | None:intergenic |
| AGAAATTCTGACGAAAGTCT+TGG | 0.505562 | 8.3:-72463866 | MS.gene07804:CDS |
| CTGCGAAACGGACTAAAGTT+GGG | 0.511483 | 8.3:-72463312 | MS.gene07804:CDS |
| AGTTAACAGATGAGGAGTAG+TGG | 0.515594 | 8.3:-72447680 | None:intergenic |
| GATAAGGAAGCCCCTGCACC+AGG | 0.518868 | 8.3:-72447714 | MS.gene07804:CDS |
| TAACCGTAGGTTTGCAGACG+AGG | 0.521173 | 8.3:-72464189 | MS.gene07804:CDS |
| GGATCGGGAGGAATCTGATA+AGG | 0.523228 | 8.3:-72447751 | MS.gene07804:CDS |
| GGAAGCCCCTGCACCAGGAG+AGG | 0.530934 | 8.3:-72447709 | MS.gene07804:CDS |
| TTCCGCCTCTCCTGGTGCAG+GGG | 0.535721 | 8.3:+72447704 | None:intergenic |
| ATGAAAAGAAAGAAATCAGT+TGG | 0.544749 | 8.3:+72463247 | None:intergenic |
| CAAAAGAACCAACTGCGAAA+CGG | 0.556686 | 8.3:-72463324 | MS.gene07804:CDS |
| CCTCCTGCTGCTGTTCAAGG+GGG | 0.558467 | 8.3:+72463971 | None:intergenic |
| GTAAGGAAGATTGCAAGAAG+TGG | 0.559179 | 8.3:-72448082 | MS.gene07804:CDS |
| GAAGTGGAGGTCTTTGACTG+AGG | 0.567533 | 8.3:-72448066 | MS.gene07804:CDS |
| TGCAGACGAGGAGGAGATCA+GGG | 0.567878 | 8.3:-72464177 | MS.gene07804:CDS |
| TGAAATAGAAGATCCTCGAC+AGG | 0.569010 | 8.3:-72464081 | MS.gene07804:intron |
| TGATTCAAAGGATGTTAAGA+GGG | 0.586389 | 8.3:-72463102 | MS.gene07804:intron |
| AACGGACTAAAGTTGGGAAA+GGG | 0.587037 | 8.3:-72463306 | MS.gene07804:CDS |
| CGGGAGGAATCTGATAAGGA+AGG | 0.587546 | 8.3:-72447747 | MS.gene07804:CDS |
| AAACGGACTAAAGTTGGGAA+AGG | 0.588435 | 8.3:-72463307 | MS.gene07804:CDS |
| GGCGGAAGAGTTAACAGATG+AGG | 0.594672 | 8.3:-72447688 | MS.gene07804:CDS |
| AGCAGCATTATACAATTCCA+TGG | 0.595262 | 8.3:+72447881 | None:intergenic |
| CCTCCTCGTCTGCAAACCTA+CGG | 0.601229 | 8.3:+72464186 | None:intergenic |
| ATATAAGAGCATACCTGTCG+AGG | 0.602504 | 8.3:+72464068 | None:intergenic |
| CTCCTGCTGCTGTTCAAGGG+GGG | 0.607441 | 8.3:+72463972 | None:intergenic |
| AATGCTCCTTGAAGCAAACA+AGG | 0.617773 | 8.3:-72464264 | MS.gene07804:CDS |
| AAATACTCGAATTTAACCGT+AGG | 0.618437 | 8.3:-72464202 | MS.gene07804:CDS |
| GGCAGAATATGAGAAGGCCA+TGG | 0.621119 | 8.3:-72447898 | MS.gene07804:CDS |
| AGGAAGATTGCAAGAAGTGG+AGG | 0.634274 | 8.3:-72448079 | MS.gene07804:CDS |
| AGCCAGACTACACAAAACTC+TGG | 0.634663 | 8.3:-72464295 | MS.gene07804:CDS |
| TATGTTTGTTCAGGATCGGG+AGG | 0.638946 | 8.3:-72447763 | MS.gene07804:intron |
| ATATTCACAAACTAAAATTG+GGG | 0.650729 | 8.3:+72463413 | None:intergenic |
| CCGTAGGTTTGCAGACGAGG+AGG | 0.664679 | 8.3:-72464186 | MS.gene07804:CDS |
| ACTCATGGCAGAATATGAGA+AGG | 0.674706 | 8.3:-72447904 | MS.gene07804:CDS |
| GAGATCAGGGAATCTAAATG+TGG | 0.675178 | 8.3:-72464164 | MS.gene07804:CDS |
| AAAGGTTAAGGATTCTAATG+CGG | 0.688426 | 8.3:-72463281 | MS.gene07804:CDS |
| GCTGAAGTGAGAAACCCGAG+AGG | 0.770934 | 8.3:-72463447 | MS.gene07804:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 72447683 | 72464330 | 72447683 | ID=MS.gene07804 |
| chr8.3 | mRNA | 72447683 | 72464330 | 72447683 | ID=MS.gene07804.t1;Parent=MS.gene07804 |
| chr8.3 | exon | 72464082 | 72464330 | 72464082 | ID=MS.gene07804.t1.exon1;Parent=MS.gene07804.t1 |
| chr8.3 | CDS | 72464082 | 72464330 | 72464082 | ID=cds.MS.gene07804.t1;Parent=MS.gene07804.t1 |
| chr8.3 | exon | 72463784 | 72464001 | 72463784 | ID=MS.gene07804.t1.exon2;Parent=MS.gene07804.t1 |
| chr8.3 | CDS | 72463784 | 72464001 | 72463784 | ID=cds.MS.gene07804.t1;Parent=MS.gene07804.t1 |
| chr8.3 | exon | 72463600 | 72463661 | 72463600 | ID=MS.gene07804.t1.exon3;Parent=MS.gene07804.t1 |
| chr8.3 | CDS | 72463600 | 72463661 | 72463600 | ID=cds.MS.gene07804.t1;Parent=MS.gene07804.t1 |
| chr8.3 | exon | 72463425 | 72463492 | 72463425 | ID=MS.gene07804.t1.exon4;Parent=MS.gene07804.t1 |
| chr8.3 | CDS | 72463425 | 72463492 | 72463425 | ID=cds.MS.gene07804.t1;Parent=MS.gene07804.t1 |
| chr8.3 | exon | 72463247 | 72463353 | 72463247 | ID=MS.gene07804.t1.exon5;Parent=MS.gene07804.t1 |
| chr8.3 | CDS | 72463247 | 72463353 | 72463247 | ID=cds.MS.gene07804.t1;Parent=MS.gene07804.t1 |
| chr8.3 | exon | 72463103 | 72463160 | 72463103 | ID=MS.gene07804.t1.exon6;Parent=MS.gene07804.t1 |
| chr8.3 | CDS | 72463103 | 72463160 | 72463103 | ID=cds.MS.gene07804.t1;Parent=MS.gene07804.t1 |
| chr8.3 | exon | 72448064 | 72448108 | 72448064 | ID=MS.gene07804.t1.exon7;Parent=MS.gene07804.t1 |
| chr8.3 | CDS | 72448064 | 72448108 | 72448064 | ID=cds.MS.gene07804.t1;Parent=MS.gene07804.t1 |
| chr8.3 | exon | 72447866 | 72447958 | 72447866 | ID=MS.gene07804.t1.exon8;Parent=MS.gene07804.t1 |
| chr8.3 | CDS | 72447866 | 72447958 | 72447866 | ID=cds.MS.gene07804.t1;Parent=MS.gene07804.t1 |
| chr8.3 | exon | 72447683 | 72447772 | 72447683 | ID=MS.gene07804.t1.exon9;Parent=MS.gene07804.t1 |
| chr8.3 | CDS | 72447683 | 72447772 | 72447683 | ID=cds.MS.gene07804.t1;Parent=MS.gene07804.t1 |
| Gene Sequence |
| Protein sequence |
