Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07901.t1 | XP_003627264.1 | 66.5 | 254 | 7 | 2 | 1 | 176 | 1 | 254 | 4.10E-78 | 300.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07901.t1 | P10290 | 50.0 | 74 | 18 | 1 | 8 | 62 | 12 | 85 | 9.7e-12 | 71.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07901.t1 | G7L6T6 | 66.5 | 254 | 7 | 2 | 1 | 176 | 1 | 254 | 2.9e-78 | 300.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene07901.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07901.t1 | MTR_8g020490 | 93.913 | 115 | 7 | 0 | 62 | 176 | 140 | 254 | 2.11e-64 | 198 |
| MS.gene07901.t1 | MTR_8g020490 | 74.390 | 82 | 2 | 1 | 1 | 63 | 1 | 82 | 2.34e-32 | 116 |
| MS.gene07901.t1 | MTR_4g125520 | 47.674 | 86 | 22 | 3 | 1 | 63 | 1 | 86 | 1.80e-16 | 75.5 |
| MS.gene07901.t1 | MTR_4g100720 | 44.000 | 75 | 23 | 1 | 8 | 63 | 13 | 87 | 1.77e-13 | 65.5 |
| MS.gene07901.t1 | MTR_3g083540 | 38.667 | 75 | 27 | 1 | 8 | 63 | 41 | 115 | 7.02e-12 | 62.8 |
| MS.gene07901.t1 | MTR_8g027345 | 40.000 | 75 | 26 | 1 | 8 | 63 | 12 | 86 | 1.56e-11 | 61.6 |
| MS.gene07901.t1 | MTR_5g079670 | 41.333 | 75 | 25 | 1 | 8 | 63 | 11 | 85 | 1.87e-11 | 60.5 |
| MS.gene07901.t1 | MTR_3g097450 | 37.209 | 86 | 31 | 2 | 1 | 63 | 1 | 86 | 6.23e-11 | 60.1 |
| MS.gene07901.t1 | MTR_4g073420 | 37.209 | 86 | 31 | 2 | 1 | 63 | 1 | 86 | 6.99e-11 | 59.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07901.t1 | AT5G35550 | 44.737 | 76 | 23 | 1 | 7 | 63 | 13 | 88 | 3.65e-14 | 68.6 |
| MS.gene07901.t1 | AT5G40330 | 39.286 | 84 | 30 | 2 | 1 | 63 | 3 | 86 | 1.90e-11 | 60.8 |
| MS.gene07901.t1 | AT4G34990 | 38.372 | 86 | 30 | 2 | 1 | 63 | 1 | 86 | 1.94e-11 | 61.2 |
| MS.gene07901.t1 | AT3G27920 | 37.662 | 77 | 29 | 1 | 6 | 63 | 12 | 88 | 2.21e-11 | 60.5 |
| MS.gene07901.t1 | AT5G14750 | 39.474 | 76 | 27 | 1 | 7 | 63 | 15 | 90 | 3.72e-11 | 59.7 |
| MS.gene07901.t1 | AT5G54230 | 41.333 | 75 | 25 | 1 | 8 | 63 | 12 | 86 | 6.18e-11 | 60.1 |
| MS.gene07901.t1 | AT3G13540 | 37.333 | 75 | 28 | 1 | 8 | 63 | 23 | 97 | 8.48e-11 | 59.3 |
Find 37 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTCATCAATTCAAAATTT+TGG | 0.180669 | 8.3:-70621636 | MS.gene07901:CDS |
| TCCATGAATGACAACATATT+TGG | 0.182285 | 8.3:+70622509 | None:intergenic |
| AAATCAGAGCACCTGCATTT+TGG | 0.210723 | 8.3:+70622463 | None:intergenic |
| CTCCTAGGCTTTGGAGCTTC+TGG | 0.233026 | 8.3:+70621668 | None:intergenic |
| ATAAGACTTCATCGTCTTCT+TGG | 0.253123 | 8.3:-70622073 | MS.gene07901:intron |
| TCCAAATATGTTGTCATTCA+TGG | 0.267011 | 8.3:-70622510 | MS.gene07901:CDS |
| GCCTTCAGTCTCCTAGGCTT+TGG | 0.359484 | 8.3:+70621659 | None:intergenic |
| TGATCATCAAGATCAATTGA+TGG | 0.381636 | 8.3:-70621543 | MS.gene07901:CDS |
| TGACCCTCAAGAGACCCTTT+TGG | 0.399906 | 8.3:-70621399 | MS.gene07901:CDS |
| ACCTTGTGATCCTCCATTTG+TGG | 0.406954 | 8.3:+70621491 | None:intergenic |
| TCATTCATGGAGAAGGAAAA+TGG | 0.417247 | 8.3:-70622497 | MS.gene07901:CDS |
| GCACCTTTATTAACCTCCTT+AGG | 0.422387 | 8.3:+70622561 | None:intergenic |
| AGTCCTAAGGAGGTTAATAA+AGG | 0.437320 | 8.3:-70622564 | MS.gene07901:CDS |
| TAATTCAAAGATTCCACAAA+TGG | 0.463789 | 8.3:-70621504 | MS.gene07901:CDS |
| GGATGTGGAGCTTAAGAAGA+TGG | 0.468435 | 8.3:-70621378 | MS.gene07901:CDS |
| CAAAAGGTTGCCCAAAATGC+AGG | 0.474230 | 8.3:-70622474 | MS.gene07901:intron |
| TCTTAAGCTCCACATCCAAA+AGG | 0.487264 | 8.3:+70621384 | None:intergenic |
| TGAACAGCCTTCAGTCTCCT+AGG | 0.499868 | 8.3:+70621653 | None:intergenic |
| CATCCAAAAGGGTCTCTTGA+GGG | 0.509517 | 8.3:+70621396 | None:intergenic |
| TATGATCATGTCCTCTTCAT+CGG | 0.523234 | 8.3:+70622093 | None:intergenic |
| TGGAGAAGGAAAATGGCAAA+AGG | 0.533124 | 8.3:-70622490 | MS.gene07901:CDS |
| CTTAAGCTCCACATCCAAAA+GGG | 0.544118 | 8.3:+70621385 | None:intergenic |
| CACCAGAAGCTCCAAAGCCT+AGG | 0.547207 | 8.3:-70621670 | MS.gene07901:CDS |
| TATGTTGTCATTCATGGAGA+AGG | 0.552003 | 8.3:-70622504 | MS.gene07901:CDS |
| TAACATGGTTAGAAGTCCTA+AGG | 0.553471 | 8.3:-70622577 | None:intergenic |
| AGGAGGTTAATAAAGGTGCT+TGG | 0.559589 | 8.3:-70622557 | MS.gene07901:CDS |
| AGCGGAAGTGAGTATGATCA+AGG | 0.564325 | 8.3:-70621605 | MS.gene07901:CDS |
| CGATATCTCTACCGATGAAG+AGG | 0.571337 | 8.3:-70622104 | MS.gene07901:CDS |
| TTCAAAGATTCCACAAATGG+AGG | 0.588987 | 8.3:-70621501 | MS.gene07901:CDS |
| TCCACAAATGGAGGATCACA+AGG | 0.589389 | 8.3:-70621492 | MS.gene07901:CDS |
| CTCAAACCAGGTATAAAGAG+AGG | 0.598083 | 8.3:-70622127 | MS.gene07901:intron |
| ATAAGATGACAATGAGCAGA+AGG | 0.600035 | 8.3:+70621434 | None:intergenic |
| CATCAAGATCAATTGATGGT+TGG | 0.610901 | 8.3:-70621539 | MS.gene07901:CDS |
| ACATCCAAAAGGGTCTCTTG+AGG | 0.616733 | 8.3:+70621395 | None:intergenic |
| TCCAAAGCCTAGGAGACTGA+AGG | 0.628232 | 8.3:-70621660 | MS.gene07901:CDS |
| CATGGTTAGAAGTCCTAAGG+AGG | 0.660711 | 8.3:-70622574 | MS.gene07901:CDS |
| GAATGACAACATATTTGGAG+AGG | 0.662814 | 8.3:+70622514 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAGTCATTTATAATAATGA+AGG | + | chr8.3:70622034-70622053 | None:intergenic | 15.0% |
| !! | TGATATTTGTTCTATATAGA+TGG | - | chr8.3:70622060-70622079 | MS.gene07901:intron | 20.0% |
| !!! | TGTTCATCAATTCAAAATTT+TGG | - | chr8.3:70622286-70622305 | MS.gene07901:intron | 20.0% |
| ! | CTAATGCACAACTATATTAT+AGG | - | chr8.3:70621485-70621504 | MS.gene07901:CDS | 25.0% |
| ! | GATAGTTAGATGAATTTGAA+CGG | - | chr8.3:70621568-70621587 | MS.gene07901:CDS | 25.0% |
| ! | TAATTCAAAGATTCCACAAA+TGG | - | chr8.3:70622418-70622437 | MS.gene07901:intron | 25.0% |
| ! | TTTCTTAAATAGCCTTCAAT+TGG | - | chr8.3:70621702-70621721 | MS.gene07901:intron | 25.0% |
| !! | TCTAACTATCAAGATTTGTT+TGG | + | chr8.3:70621558-70621577 | None:intergenic | 25.0% |
| !!! | AAAATTTTGGAGAAGAATAG+CGG | - | chr8.3:70622299-70622318 | MS.gene07901:intron | 25.0% |
| ACAATGAAATCAAGAACTAC+TGG | - | chr8.3:70622117-70622136 | MS.gene07901:CDS | 30.0% | |
| ACTTGACATAAGTCTCATAA+TGG | - | chr8.3:70621926-70621945 | MS.gene07901:intron | 30.0% | |
| CAAATCAGATTCGAAATCTA+AGG | + | chr8.3:70621648-70621667 | None:intergenic | 30.0% | |
| CTTAGATTTCGAATCTGATT+TGG | - | chr8.3:70621646-70621665 | MS.gene07901:CDS | 30.0% | |
| TATATGGAGTAGGTTTAAAG+CGG | - | chr8.3:70621736-70621755 | MS.gene07901:intron | 30.0% | |
| TCCAAATATGTTGTCATTCA+TGG | - | chr8.3:70621412-70621431 | MS.gene07901:CDS | 30.0% | |
| TCCATGAATGACAACATATT+TGG | + | chr8.3:70621416-70621435 | None:intergenic | 30.0% | |
| TCTCATGAAAACACATTGAA+TGG | + | chr8.3:70621514-70621533 | None:intergenic | 30.0% | |
| TGATCATCAAGATCAATTGA+TGG | - | chr8.3:70622379-70622398 | MS.gene07901:intron | 30.0% | |
| ! | AATGATGATGATGATGATGT+TGG | + | chr8.3:70622176-70622195 | None:intergenic | 30.0% |
| ! | ATTGGTCTCTTTGGTTTATA+TGG | - | chr8.3:70621720-70621739 | MS.gene07901:intron | 30.0% |
| AAACCAAAGAGACCAATTGA+AGG | + | chr8.3:70621717-70621736 | None:intergenic | 35.0% | |
| AGTCCTAAGGAGGTTAATAA+AGG | - | chr8.3:70621358-70621377 | MS.gene07901:CDS | 35.0% | |
| ATAAGACTTCATCGTCTTCT+TGG | - | chr8.3:70621849-70621868 | MS.gene07901:intron | 35.0% | |
| ATAAGATGACAATGAGCAGA+AGG | + | chr8.3:70622491-70622510 | None:intergenic | 35.0% | |
| CAACACAATCATCACAAATG+TGG | - | chr8.3:70622221-70622240 | MS.gene07901:intron | 35.0% | |
| CAACGATGATTATTCTAGTC+AGG | - | chr8.3:70621984-70622003 | MS.gene07901:intron | 35.0% | |
| GAAAAAGTTGCAGACAAAGA+TGG | - | chr8.3:70621763-70621782 | MS.gene07901:intron | 35.0% | |
| GAATGACAACATATTTGGAG+AGG | + | chr8.3:70621411-70621430 | None:intergenic | 35.0% | |
| TAAGTCTCATAATGGTGATC+TGG | - | chr8.3:70621934-70621953 | MS.gene07901:intron | 35.0% | |
| TATGATCATGTCCTCTTCAT+CGG | + | chr8.3:70621832-70621851 | None:intergenic | 35.0% | |
| TATGTTGTCATTCATGGAGA+AGG | - | chr8.3:70621418-70621437 | MS.gene07901:CDS | 35.0% | |
| TCATTCATGGAGAAGGAAAA+TGG | - | chr8.3:70621425-70621444 | MS.gene07901:CDS | 35.0% | |
| TGGTTGAATTATCTCAAACC+AGG | - | chr8.3:70621783-70621802 | MS.gene07901:intron | 35.0% | |
| TTCAAAGATTCCACAAATGG+AGG | - | chr8.3:70622421-70622440 | MS.gene07901:intron | 35.0% | |
| ! | AAGAAGATGGCTTCTTTTCT+TGG | - | chr8.3:70622557-70622576 | MS.gene07901:CDS | 35.0% |
| ! | CATCAAGATCAATTGATGGT+TGG | - | chr8.3:70622383-70622402 | MS.gene07901:intron | 35.0% |
| ! | CTCTTTGGTTTATATGGAGT+AGG | - | chr8.3:70621726-70621745 | MS.gene07901:intron | 35.0% |
| AGTGAAACAGAAGATCGTGA+TGG | + | chr8.3:70622200-70622219 | None:intergenic | 40.0% | |
| ATATCGCCTCTCTTTATACC+TGG | + | chr8.3:70621804-70621823 | None:intergenic | 40.0% | |
| ATTTCATTGTCTGTTCGTCC+TGG | + | chr8.3:70622107-70622126 | None:intergenic | 40.0% | |
| CTCAAACCAGGTATAAAGAG+AGG | - | chr8.3:70621795-70621814 | MS.gene07901:intron | 40.0% | |
| CTTAAGCTCCACATCCAAAA+GGG | + | chr8.3:70622540-70622559 | None:intergenic | 40.0% | |
| GAACAATCTTGTATCCTCTG+CGG | + | chr8.3:70621616-70621635 | None:intergenic | 40.0% | |
| GCACCTTTATTAACCTCCTT+AGG | + | chr8.3:70621364-70621383 | None:intergenic | 40.0% | |
| TCGAAATCTAAGGACATGCA+TGG | + | chr8.3:70621638-70621657 | None:intergenic | 40.0% | |
| TCTTAAGCTCCACATCCAAA+AGG | + | chr8.3:70622541-70622560 | None:intergenic | 40.0% | |
| TGGAGAAGGAAAATGGCAAA+AGG | - | chr8.3:70621432-70621451 | MS.gene07901:CDS | 40.0% | |
| TTTGTTTGGAGAGACAGCAA+AGG | + | chr8.3:70621544-70621563 | None:intergenic | 40.0% | |
| ! | CATAATGGTGATCTGGAGTT+CGG | - | chr8.3:70621941-70621960 | MS.gene07901:intron | 40.0% |
| ! | GATGGTCATTGATAGCTAAG+AGG | - | chr8.3:70622078-70622097 | MS.gene07901:CDS | 40.0% |
| ! | TAGCCTTCAATTGGTCTCTT+TGG | - | chr8.3:70621711-70621730 | MS.gene07901:intron | 40.0% |
| ! | TTCATCGTCTTCTTGGTAAC+AGG | - | chr8.3:70621856-70621875 | MS.gene07901:intron | 40.0% |
| !! | AAATCAGAGCACCTGCATTT+TGG | + | chr8.3:70621462-70621481 | None:intergenic | 40.0% |
| !! | AATCAGAGCACCTGCATTTT+GGG | + | chr8.3:70621461-70621480 | None:intergenic | 40.0% |
| !! | AGGAGGTTAATAAAGGTGCT+TGG | - | chr8.3:70621365-70621384 | MS.gene07901:CDS | 40.0% |
| ACATCCAAAAGGGTCTCTTG+AGG | + | chr8.3:70622530-70622549 | None:intergenic | 45.0% | |
| ACCTTGTGATCCTCCATTTG+TGG | + | chr8.3:70622434-70622453 | None:intergenic | 45.0% | |
| AGCGGAAGTGAGTATGATCA+AGG | - | chr8.3:70622317-70622336 | MS.gene07901:intron | 45.0% | |
| CAAAAGGTTGCCCAAAATGC+AGG | - | chr8.3:70621448-70621467 | MS.gene07901:CDS | 45.0% | |
| CATCCAAAAGGGTCTCTTGA+GGG | + | chr8.3:70622529-70622548 | None:intergenic | 45.0% | |
| CGATATCTCTACCGATGAAG+AGG | - | chr8.3:70621818-70621837 | MS.gene07901:intron | 45.0% | |
| GACAACATGATCTTCTGCCA+CGG | + | chr8.3:70621672-70621691 | None:intergenic | 45.0% | |
| GGATGTGGAGCTTAAGAAGA+TGG | - | chr8.3:70622544-70622563 | MS.gene07901:CDS | 45.0% | |
| TCCACAAATGGAGGATCACA+AGG | - | chr8.3:70622430-70622449 | MS.gene07901:intron | 45.0% | |
| ! | TCAAGAGACCCTTTTGGATG+TGG | - | chr8.3:70622529-70622548 | MS.gene07901:CDS | 45.0% |
| ! | TTGATAGCTAAGAGGCTACC+AGG | - | chr8.3:70622086-70622105 | MS.gene07901:CDS | 45.0% |
| !! | TTTCGAATCTGATTTGGCCG+TGG | - | chr8.3:70621652-70621671 | MS.gene07901:CDS | 45.0% |
| GATGAATTTGAACGGCCGAG+AGG | - | chr8.3:70621576-70621595 | MS.gene07901:CDS | 50.0% | |
| GTCTCTCCAGACAAACCTCT+CGG | + | chr8.3:70621594-70621613 | None:intergenic | 50.0% | |
| TCCAAAGCCTAGGAGACTGA+AGG | - | chr8.3:70622262-70622281 | MS.gene07901:intron | 50.0% | |
| TGAACAGCCTTCAGTCTCCT+AGG | + | chr8.3:70622272-70622291 | None:intergenic | 50.0% | |
| ! | GGAGTAGGTTTAAAGCGGTG+TGG | - | chr8.3:70621741-70621760 | MS.gene07901:intron | 50.0% |
| ! | TGACCCTCAAGAGACCCTTT+TGG | - | chr8.3:70622523-70622542 | MS.gene07901:CDS | 50.0% |
| CACCAGAAGCTCCAAAGCCT+AGG | - | chr8.3:70622252-70622271 | MS.gene07901:intron | 55.0% | |
| GCCTTCAGTCTCCTAGGCTT+TGG | + | chr8.3:70622266-70622285 | None:intergenic | 55.0% | |
| TTTGTCTGGAGAGACCGCAG+AGG | - | chr8.3:70621599-70621618 | MS.gene07901:CDS | 55.0% | |
| !! | CTCCTAGGCTTTGGAGCTTC+TGG | + | chr8.3:70622257-70622276 | None:intergenic | 55.0% |
| GAACGGCCGAGAGGTTTGTC+TGG | - | chr8.3:70621585-70621604 | MS.gene07901:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.3 | gene | 70621349 | 70622595 | 70621349 | ID=MS.gene07901 |
| chr8.3 | mRNA | 70621349 | 70622595 | 70621349 | ID=MS.gene07901.t1;Parent=MS.gene07901 |
| chr8.3 | exon | 70622475 | 70622595 | 70622475 | ID=MS.gene07901.t1.exon1;Parent=MS.gene07901.t1 |
| chr8.3 | CDS | 70622475 | 70622595 | 70622475 | ID=cds.MS.gene07901.t1;Parent=MS.gene07901.t1 |
| chr8.3 | exon | 70622074 | 70622139 | 70622074 | ID=MS.gene07901.t1.exon2;Parent=MS.gene07901.t1 |
| chr8.3 | CDS | 70622074 | 70622139 | 70622074 | ID=cds.MS.gene07901.t1;Parent=MS.gene07901.t1 |
| chr8.3 | exon | 70621349 | 70621692 | 70621349 | ID=MS.gene07901.t1.exon3;Parent=MS.gene07901.t1 |
| chr8.3 | CDS | 70621349 | 70621692 | 70621349 | ID=cds.MS.gene07901.t1;Parent=MS.gene07901.t1 |
| Gene Sequence |
| Protein sequence |
