Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08086.t1 | XP_003590850.2 | 74.3 | 642 | 95 | 4 | 1 | 581 | 1 | 633 | 6.30E-261 | 909.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08086.t1 | Q9LUZ4 | 48.3 | 590 | 257 | 19 | 21 | 578 | 2 | 575 | 9.6e-133 | 475.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08086.t1 | G7I4W5 | 74.3 | 642 | 95 | 4 | 1 | 581 | 1 | 633 | 4.6e-261 | 909.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene08086.t1 | TF | C3H |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08086.t1 | MTR_1g075890 | 74.028 | 643 | 97 | 4 | 1 | 582 | 1 | 634 | 0.0 | 891 |
| MS.gene08086.t1 | MTR_3g464280 | 66.551 | 574 | 111 | 7 | 1 | 574 | 1 | 493 | 0.0 | 692 |
| MS.gene08086.t1 | MTR_3g464260 | 67.684 | 557 | 78 | 8 | 27 | 580 | 21 | 478 | 0.0 | 688 |
| MS.gene08086.t1 | MTR_5g071070 | 53.951 | 658 | 215 | 15 | 1 | 578 | 1 | 650 | 0.0 | 613 |
| MS.gene08086.t1 | MTR_5g071070 | 54.688 | 576 | 175 | 15 | 81 | 578 | 1 | 568 | 0.0 | 534 |
| MS.gene08086.t1 | MTR_5g082830 | 47.360 | 625 | 237 | 15 | 28 | 578 | 25 | 631 | 5.12e-174 | 509 |
| MS.gene08086.t1 | MTR_7g092070 | 41.827 | 624 | 258 | 15 | 33 | 560 | 47 | 661 | 3.71e-139 | 421 |
| MS.gene08086.t1 | MTR_3g070880 | 42.730 | 564 | 208 | 15 | 33 | 578 | 10 | 476 | 9.73e-131 | 392 |
| MS.gene08086.t1 | MTR_3g464290 | 55.582 | 421 | 141 | 9 | 175 | 582 | 54 | 441 | 9.72e-125 | 375 |
| MS.gene08086.t1 | MTR_3g029590 | 39.070 | 645 | 287 | 17 | 33 | 576 | 45 | 684 | 1.83e-123 | 380 |
| MS.gene08086.t1 | MTR_3g464270 | 55.256 | 371 | 125 | 8 | 220 | 578 | 8 | 349 | 4.75e-115 | 346 |
| MS.gene08086.t1 | MTR_1g050438 | 39.872 | 469 | 227 | 10 | 33 | 458 | 15 | 471 | 5.56e-98 | 310 |
| MS.gene08086.t1 | MTR_0728s0020 | 38.710 | 496 | 245 | 11 | 10 | 458 | 4 | 487 | 9.18e-98 | 311 |
| MS.gene08086.t1 | MTR_3g435590 | 46.369 | 358 | 173 | 4 | 33 | 372 | 32 | 388 | 3.32e-95 | 299 |
| MS.gene08086.t1 | MTR_3g435680 | 56.742 | 178 | 68 | 4 | 198 | 372 | 130 | 301 | 2.19e-63 | 214 |
| MS.gene08086.t1 | MTR_8g102100 | 52.439 | 164 | 72 | 1 | 217 | 374 | 77 | 240 | 6.48e-53 | 185 |
| MS.gene08086.t1 | MTR_6g090380 | 59.091 | 132 | 45 | 1 | 192 | 323 | 83 | 205 | 4.57e-52 | 183 |
| MS.gene08086.t1 | MTR_3g103960 | 58.955 | 134 | 53 | 2 | 211 | 342 | 43 | 176 | 2.36e-49 | 172 |
| MS.gene08086.t1 | MTR_2g090605 | 59.459 | 111 | 45 | 0 | 221 | 331 | 77 | 187 | 3.41e-46 | 168 |
| MS.gene08086.t1 | MTR_4g078660 | 56.075 | 107 | 47 | 0 | 217 | 323 | 56 | 162 | 1.02e-43 | 159 |
| MS.gene08086.t1 | MTR_1g081420 | 83.333 | 48 | 8 | 0 | 492 | 539 | 181 | 228 | 1.62e-18 | 85.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08086.t1 | AT5G58620 | 48.211 | 587 | 257 | 18 | 24 | 578 | 4 | 575 | 1.32e-166 | 488 |
| MS.gene08086.t1 | AT2G40140 | 45.470 | 596 | 257 | 17 | 1 | 578 | 1 | 546 | 1.20e-153 | 454 |
| MS.gene08086.t1 | AT2G40140 | 45.470 | 596 | 257 | 17 | 1 | 578 | 1 | 546 | 1.20e-153 | 454 |
| MS.gene08086.t1 | AT3G55980 | 44.370 | 595 | 257 | 17 | 1 | 578 | 1 | 538 | 2.47e-150 | 445 |
| MS.gene08086.t1 | AT3G55980 | 44.370 | 595 | 257 | 17 | 1 | 578 | 68 | 605 | 1.53e-149 | 445 |
| MS.gene08086.t1 | AT5G12850 | 41.063 | 621 | 262 | 20 | 25 | 557 | 34 | 638 | 4.15e-132 | 402 |
| MS.gene08086.t1 | AT2G41900 | 46.970 | 462 | 187 | 10 | 33 | 454 | 48 | 491 | 1.24e-128 | 394 |
| MS.gene08086.t1 | AT5G07500 | 61.157 | 121 | 47 | 0 | 203 | 323 | 34 | 154 | 3.30e-52 | 179 |
| MS.gene08086.t1 | AT4G29190 | 48.876 | 178 | 69 | 3 | 210 | 380 | 68 | 230 | 7.52e-52 | 182 |
| MS.gene08086.t1 | AT2G25900 | 57.364 | 129 | 55 | 0 | 217 | 345 | 86 | 214 | 3.23e-50 | 176 |
| MS.gene08086.t1 | AT2G25900 | 57.364 | 129 | 55 | 0 | 217 | 345 | 86 | 214 | 3.23e-50 | 176 |
| MS.gene08086.t1 | AT2G19810 | 61.475 | 122 | 45 | 1 | 206 | 325 | 61 | 182 | 3.74e-50 | 177 |
| MS.gene08086.t1 | AT1G03790 | 51.592 | 157 | 68 | 3 | 186 | 338 | 51 | 203 | 4.04e-48 | 172 |
| MS.gene08086.t1 | AT5G44260 | 57.031 | 128 | 50 | 2 | 199 | 325 | 42 | 165 | 2.07e-45 | 165 |
Find 138 sgRNAs with CRISPR-Local
Find 154 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTCTTCATTGTGCCGTTTC+AGG | 0.147704 | 3.3:-49550188 | MS.gene08086:CDS |
| CTTATGGTTGCTGCCTTATT+TGG | 0.182687 | 3.3:-49550290 | MS.gene08086:CDS |
| GCCTTCTACCTTCTCTGGTT+GGG | 0.241460 | 3.3:-49548956 | MS.gene08086:CDS |
| TTGTCAAACTAGTTTGATTA+AGG | 0.245776 | 3.3:-49550501 | MS.gene08086:CDS |
| GCAAAGTGAAGCCTTGTTCT+AGG | 0.263559 | 3.3:-49549848 | MS.gene08086:CDS |
| GGTGTGTTGTCTTATATTCT+TGG | 0.275343 | 3.3:-49550260 | MS.gene08086:CDS |
| TTGATTAAGGAAAATGAATA+TGG | 0.284568 | 3.3:-49550488 | MS.gene08086:CDS |
| CACACCCTTGCTTCCAAATA+AGG | 0.291991 | 3.3:+49550277 | None:intergenic |
| TATATAGTACCGATGAATTT+AGG | 0.298879 | 3.3:-49549881 | MS.gene08086:CDS |
| TTCTCGGCATTTGAAGGGTT+GGG | 0.308891 | 3.3:+49549352 | None:intergenic |
| GAACGAAAACCAACAACTTT+GGG | 0.309040 | 3.3:-49549130 | MS.gene08086:CDS |
| CTAGGGCTTATTCTCATGAT+TGG | 0.328400 | 3.3:-49549830 | MS.gene08086:CDS |
| GAAAGCCATTACATCATCTT+TGG | 0.329332 | 3.3:+49550415 | None:intergenic |
| ACTCCTGCAAGCTTATTTGA+TGG | 0.329612 | 3.3:+49549222 | None:intergenic |
| GTCTACCTTGCCCCGAGTTT+CGG | 0.332508 | 3.3:-49549737 | MS.gene08086:CDS |
| TTTGAAGTGGTAAGATTAGA+AGG | 0.343131 | 3.3:+49549102 | None:intergenic |
| CTTGTTGCTGCTCCACCGTT+TGG | 0.345377 | 3.3:+49549976 | None:intergenic |
| ATGGAGCCTTCTACCTTCTC+TGG | 0.362092 | 3.3:-49548961 | MS.gene08086:CDS |
| TCTCCATCAAATAAGCTTGC+AGG | 0.370221 | 3.3:-49549225 | MS.gene08086:CDS |
| ATCTGTTTCTCGGCATTTGA+AGG | 0.372092 | 3.3:+49549346 | None:intergenic |
| AGCAAAGAAGCAAACCCTTC+TGG | 0.372700 | 3.3:+49549602 | None:intergenic |
| TTCTGCTACCTTAGTTGCCA+TGG | 0.385179 | 3.3:-49548980 | MS.gene08086:CDS |
| CTAATAGTATCTTCGGTTCA+AGG | 0.397475 | 3.3:-49550055 | MS.gene08086:CDS |
| TGAAGAGAGGACACCTCTTA+TGG | 0.398546 | 3.3:-49550306 | MS.gene08086:CDS |
| CTTCCTTGCAAAGCCTTGTT+CGG | 0.403908 | 3.3:+49549637 | None:intergenic |
| TTCACCTAGCGCTTCTTCAA+TGG | 0.404852 | 3.3:-49549505 | MS.gene08086:CDS |
| TTGTCTTATATTCTTGGAAC+CGG | 0.405812 | 3.3:-49550254 | MS.gene08086:CDS |
| TTTCTCGGCATTTGAAGGGT+TGG | 0.421787 | 3.3:+49549351 | None:intergenic |
| TAATCTTTCTTCTCAGTTCG+AGG | 0.423342 | 3.3:+49549942 | None:intergenic |
| CGAGTGTCCATTTGTTCACC+CGG | 0.428988 | 3.3:-49549805 | MS.gene08086:CDS |
| TCGGTTAACATCGACATAAC+CGG | 0.432174 | 3.3:+49550235 | None:intergenic |
| TCGGTTTCTAATAGTATCTT+CGG | 0.438190 | 3.3:-49550062 | MS.gene08086:CDS |
| AAGGATAACCCCAAAGTTGT+TGG | 0.439414 | 3.3:+49549121 | None:intergenic |
| AGAACGAAAACCAACAACTT+TGG | 0.439453 | 3.3:-49549131 | MS.gene08086:CDS |
| CCCCAACCAGAGAAGGTAGA+AGG | 0.441981 | 3.3:+49548955 | None:intergenic |
| CCTTCTACCTTCTCTGGTTG+GGG | 0.443126 | 3.3:-49548955 | MS.gene08086:CDS |
| AGGAAGAGGATACTACTAGA+AGG | 0.443626 | 3.3:-49550035 | MS.gene08086:CDS |
| CCATCATTAGCTTACACATA+AGG | 0.444911 | 3.3:+49549268 | None:intergenic |
| TTCAGCCAAAGATGATGTAA+TGG | 0.447315 | 3.3:-49550420 | MS.gene08086:CDS |
| CCCTATCTGAGCCGCACACT+CGG | 0.447918 | 3.3:+49550216 | None:intergenic |
| GCAAACCCTTCTGGTGCATC+CGG | 0.454052 | 3.3:+49549611 | None:intergenic |
| CAGTTGATATGAACGGTAAC+CGG | 0.454347 | 3.3:-49550103 | MS.gene08086:CDS |
| GCACGACCCTTTCCGAAACT+CGG | 0.454722 | 3.3:+49549725 | None:intergenic |
| AGCCTTCTACCTTCTCTGGT+TGG | 0.455905 | 3.3:-49548957 | MS.gene08086:CDS |
| GCAGCATCATCGCATGCAGC+TGG | 0.462576 | 3.3:-49549414 | MS.gene08086:CDS |
| TGCTGCCTTATTTGGAAGCA+AGG | 0.473427 | 3.3:-49550282 | MS.gene08086:CDS |
| CACGACCCTTTCCGAAACTC+GGG | 0.473623 | 3.3:+49549726 | None:intergenic |
| GAGGATTGGCTCAAAGGAAA+TGG | 0.473772 | 3.3:-49550333 | MS.gene08086:CDS |
| TCTGGTTGGGGATCCCCTGA+TGG | 0.473856 | 3.3:-49548943 | MS.gene08086:CDS |
| AGAAGGCTCCATGGCAACTA+AGG | 0.474619 | 3.3:+49548972 | None:intergenic |
| ACCGAGTGTGCGGCTCAGAT+AGG | 0.476494 | 3.3:-49550217 | MS.gene08086:CDS |
| TATATCCCATTCATTATGTC+AGG | 0.477387 | 3.3:+49549900 | None:intergenic |
| AATAAGGCAGCAACCATAAG+AGG | 0.478339 | 3.3:+49550293 | None:intergenic |
| TCCCTACCTGACATAATGAA+TGG | 0.480887 | 3.3:-49549906 | MS.gene08086:CDS |
| CTTACCACTTCAAATATGAA+TGG | 0.481357 | 3.3:-49549093 | MS.gene08086:CDS |
| AGCAGCAGAACAACCTGAAA+CGG | 0.481598 | 3.3:+49550175 | None:intergenic |
| TACAACGGTCGAAGCTCCTC+TGG | 0.485266 | 3.3:+49549573 | None:intergenic |
| CCCTACCTGACATAATGAAT+GGG | 0.488450 | 3.3:-49549905 | MS.gene08086:CDS |
| AGGCTTTGCAAGGAAGGAAC+CGG | 0.488722 | 3.3:-49549630 | MS.gene08086:CDS |
| CGACCGTTGTATGCTTCTAC+CGG | 0.490079 | 3.3:-49549561 | MS.gene08086:CDS |
| CAAAGTGAAGCCTTGTTCTA+GGG | 0.493230 | 3.3:-49549847 | MS.gene08086:CDS |
| AGCCTTGTTCGGTATTGAGC+AGG | 0.501073 | 3.3:+49549648 | None:intergenic |
| TTATGGTATGGAAGGAGGAT+TGG | 0.501743 | 3.3:-49550347 | MS.gene08086:CDS |
| AGGTGAAGGCAAAGCAGAAC+CGG | 0.502979 | 3.3:+49549542 | None:intergenic |
| TTCTGCGCTCAATAAAGCTC+TGG | 0.504121 | 3.3:+49549031 | None:intergenic |
| ATTGAGTTCATCGCCGCAGA+TGG | 0.509619 | 3.3:+49548906 | None:intergenic |
| CCGAGTGTGCGGCTCAGATA+GGG | 0.511710 | 3.3:-49550216 | MS.gene08086:CDS |
| CGAACAAGGCTTTGCAAGGA+AGG | 0.512498 | 3.3:-49549636 | MS.gene08086:CDS |
| TTGCTTGATGCATCTGCAGA+TGG | 0.513899 | 3.3:-49550131 | MS.gene08086:CDS |
| CCTTATGTGTAAGCTAATGA+TGG | 0.517096 | 3.3:-49549268 | MS.gene08086:CDS |
| CTTGCCCCGAGTTTCGGAAA+GGG | 0.522774 | 3.3:-49549731 | MS.gene08086:CDS |
| GTCATGTGCAACGGTGCCAA+TGG | 0.528443 | 3.3:-49550530 | MS.gene08086:CDS |
| GTATCTTCGGTTCAAGGAAG+AGG | 0.528667 | 3.3:-49550049 | MS.gene08086:CDS |
| CCCATTCATTATGTCAGGTA+GGG | 0.528714 | 3.3:+49549905 | None:intergenic |
| TTCATTATGTCAGGTAGGGA+TGG | 0.535137 | 3.3:+49549909 | None:intergenic |
| CCTTGCCCCGAGTTTCGGAA+AGG | 0.536756 | 3.3:-49549732 | MS.gene08086:CDS |
| TCTGTTTCTCGGCATTTGAA+GGG | 0.538648 | 3.3:+49549347 | None:intergenic |
| GCTGCCTTATTTGGAAGCAA+GGG | 0.541989 | 3.3:-49550281 | MS.gene08086:CDS |
| GAAGAAATTAGGTTTCCAAA+CGG | 0.542543 | 3.3:-49549991 | MS.gene08086:CDS |
| TGGAAGGAGGATTGGCTCAA+AGG | 0.543834 | 3.3:-49550339 | MS.gene08086:CDS |
| GGAAGAGGATACTACTAGAA+GGG | 0.548805 | 3.3:-49550034 | MS.gene08086:CDS |
| GATGGAGTCAAAGGTGGTGT+CGG | 0.552343 | 3.3:+49549438 | None:intergenic |
| CAGAAGTAGCACTTCAACAA+TGG | 0.554783 | 3.3:-49548848 | MS.gene08086:CDS |
| ATGTTGATGAGGTAGGGTTA+TGG | 0.554961 | 3.3:-49550364 | MS.gene08086:CDS |
| CGGGTGAACAAATGGACACT+CGG | 0.558339 | 3.3:+49549806 | None:intergenic |
| GTGTAGCGACTTGATTGTCT+CGG | 0.560342 | 3.3:-49550081 | MS.gene08086:CDS |
| CCCCTGATGGAAAATTAGAC+TGG | 0.563187 | 3.3:-49548930 | MS.gene08086:CDS |
| CCTTTCCGAAACTCGGGGCA+AGG | 0.566549 | 3.3:+49549732 | None:intergenic |
| GGTGAATTTGAATATGATGT+AGG | 0.568172 | 3.3:+49549522 | None:intergenic |
| GATCATGATGTTGATGAGGT+AGG | 0.571630 | 3.3:-49550371 | MS.gene08086:CDS |
| ATCATGATGTTGATGAGGTA+GGG | 0.573336 | 3.3:-49550370 | MS.gene08086:CDS |
| TTGTTCTGCTGCTTCTGCTG+AGG | 0.573966 | 3.3:-49550165 | MS.gene08086:CDS |
| TGTAAACATCCTAAATTCAT+CGG | 0.575359 | 3.3:+49549872 | None:intergenic |
| ATCCTGCTCAATACCGAACA+AGG | 0.577844 | 3.3:-49549650 | MS.gene08086:CDS |
| CTTCTTTGCTCACAAACCAG+AGG | 0.579205 | 3.3:-49549589 | MS.gene08086:CDS |
| TAGGGTTATGGTATGGAAGG+AGG | 0.579481 | 3.3:-49550352 | MS.gene08086:CDS |
| AGGGGATCCCCAACCAGAGA+AGG | 0.582505 | 3.3:+49548948 | None:intergenic |
| TGATCCATTCATATTTGAAG+TGG | 0.582834 | 3.3:+49549089 | None:intergenic |
| GAAATTAGGTTTCCAAACGG+TGG | 0.587095 | 3.3:-49549988 | MS.gene08086:CDS |
| TAAGGTAGCAGAAGGAAGCT+CGG | 0.593413 | 3.3:+49548990 | None:intergenic |
| ATACCGAACAAGGCTTTGCA+AGG | 0.594223 | 3.3:-49549640 | MS.gene08086:CDS |
| GAAGAGGATACTACTAGAAG+GGG | 0.595681 | 3.3:-49550033 | MS.gene08086:CDS |
| GCATGCGATGATGCTGCAGA+TGG | 0.598498 | 3.3:+49549420 | None:intergenic |
| AGGTAGGGTTATGGTATGGA+AGG | 0.598568 | 3.3:-49550355 | MS.gene08086:CDS |
| GAGTGTCCATTTGTTCACCC+GGG | 0.599662 | 3.3:-49549804 | MS.gene08086:CDS |
| GTCGATGTTAACCGAGTGTG+CGG | 0.601717 | 3.3:-49550227 | MS.gene08086:CDS |
| TCCCATTCATTATGTCAGGT+AGG | 0.601794 | 3.3:+49549904 | None:intergenic |
| GCGATGAACTCAATAAAATG+AGG | 0.603206 | 3.3:-49548897 | MS.gene08086:CDS |
| AGACAATCAAGTCGCTACAC+CGG | 0.604044 | 3.3:+49550084 | None:intergenic |
| GATGAGGTAGGGTTATGGTA+TGG | 0.604921 | 3.3:-49550359 | MS.gene08086:CDS |
| AAGGAACCGGATGCACCAGA+AGG | 0.605474 | 3.3:-49549617 | MS.gene08086:CDS |
| GTGGAGCAGCAACAAGATGT+AGG | 0.608133 | 3.3:-49549969 | MS.gene08086:CDS |
| AACGAAAACCAACAACTTTG+GGG | 0.608292 | 3.3:-49549129 | MS.gene08086:CDS |
| TGATACAACTGCATTGATGT+CGG | 0.609284 | 3.3:+49549153 | None:intergenic |
| AGGAAATGGCATGTGAAGAG+AGG | 0.615831 | 3.3:-49550319 | MS.gene08086:CDS |
| ATGGCAACTAAGGTAGCAGA+AGG | 0.616236 | 3.3:+49548982 | None:intergenic |
| GAATCCATTGAAGAAGCGCT+AGG | 0.617474 | 3.3:+49549501 | None:intergenic |
| AGAGAAGGTAGAAGGCTCCA+TGG | 0.618438 | 3.3:+49548963 | None:intergenic |
| TGAGAATAAGCCCTAGAACA+AGG | 0.619488 | 3.3:+49549837 | None:intergenic |
| AGTGTCCATTTGTTCACCCG+GGG | 0.625388 | 3.3:-49549803 | MS.gene08086:CDS |
| CTAATGATGGAAGAGAAGAC+AGG | 0.628766 | 3.3:-49549255 | MS.gene08086:CDS |
| AAAATCAAAGTCATGTGCAA+CGG | 0.635454 | 3.3:-49550539 | None:intergenic |
| CTGAATCTGAGTCTGCCACA+TGG | 0.636502 | 3.3:+49549389 | None:intergenic |
| TTTGAATATGATGTAGGTGA+AGG | 0.636602 | 3.3:+49549528 | None:intergenic |
| GGTAGTTCAGTTGATATGAA+CGG | 0.639398 | 3.3:-49550110 | MS.gene08086:CDS |
| AGGAACCGGATGCACCAGAA+GGG | 0.647933 | 3.3:-49549616 | MS.gene08086:CDS |
| TTAATCAAACTAGTTTGACA+AGG | 0.649059 | 3.3:+49550503 | None:intergenic |
| GATGCTGCAGATGGAGTCAA+AGG | 0.650089 | 3.3:+49549429 | None:intergenic |
| AGATGATCATGATGTTGATG+AGG | 0.655942 | 3.3:-49550375 | MS.gene08086:CDS |
| AAATTAGACTGGTCCATCTG+CGG | 0.658201 | 3.3:-49548919 | MS.gene08086:CDS |
| ACGACCCTTTCCGAAACTCG+GGG | 0.660326 | 3.3:+49549727 | None:intergenic |
| AATGCATCAACTCTAAATTG+TGG | 0.663405 | 3.3:+49549066 | None:intergenic |
| GCTGCAGATGGAGTCAAAGG+TGG | 0.704806 | 3.3:+49549432 | None:intergenic |
| AATGGTTGTTGCATCAAACG+TGG | 0.708040 | 3.3:-49548830 | MS.gene08086:CDS |
| GTGTCCATTTGTTCACCCGG+GGG | 0.714093 | 3.3:-49549802 | MS.gene08086:CDS |
| CGCATGCAGCTGGAACCATG+TGG | 0.722861 | 3.3:-49549404 | MS.gene08086:CDS |
| GATGCTTGTGAATATGCACA+TGG | 0.753418 | 3.3:-49549693 | MS.gene08086:CDS |
| GAACCGGTAGAAGCATACAA+CGG | 0.780700 | 3.3:+49549558 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTGATTAAGGAAAATGAATA+TGG | - | chr3.3:49548840-49548859 | MS.gene08086:CDS | 20.0% |
| !!! | TATTGGCTTAAAAATATATC+AGG | + | chr3.3:49550529-49550548 | None:intergenic | 20.0% |
| ! | TGTAAACATCCTAAATTCAT+CGG | + | chr3.3:49549459-49549478 | None:intergenic | 25.0% |
| !! | ACTAACCTTAAAAACACTTT+TGG | - | chr3.3:49550136-49550155 | MS.gene08086:CDS | 25.0% |
| !! | CATGTGTAATGATATTTTCT+CGG | + | chr3.3:49549572-49549591 | None:intergenic | 25.0% |
| !! | CTAACCTTAAAAACACTTTT+GGG | - | chr3.3:49550137-49550156 | MS.gene08086:CDS | 25.0% |
| !! | GCAGATTTTATCAAAAAAGA+CGG | + | chr3.3:49549869-49549888 | None:intergenic | 25.0% |
| !! | TTAATCAAACTAGTTTGACA+AGG | + | chr3.3:49548828-49548847 | None:intergenic | 25.0% |
| !! | TTGTCAAACTAGTTTGATTA+AGG | - | chr3.3:49548827-49548846 | MS.gene08086:CDS | 25.0% |
| !!! | AAAGTGTTTTTAAGGTTAGT+AGG | + | chr3.3:49550136-49550155 | None:intergenic | 25.0% |
| !!! | ATTTTCAAGTTCGATACATT+CGG | + | chr3.3:49550038-49550057 | None:intergenic | 25.0% |
| !!! | TATATAGTACCGATGAATTT+AGG | - | chr3.3:49549447-49549466 | MS.gene08086:CDS | 25.0% |
| AATGCATCAACTCTAAATTG+TGG | + | chr3.3:49550265-49550284 | None:intergenic | 30.0% | |
| CTTACCACTTCAAATATGAA+TGG | - | chr3.3:49550235-49550254 | MS.gene08086:CDS | 30.0% | |
| GAAGAAATTAGGTTTCCAAA+CGG | - | chr3.3:49549337-49549356 | MS.gene08086:CDS | 30.0% | |
| GGTGAATTTGAATATGATGT+AGG | + | chr3.3:49549809-49549828 | None:intergenic | 30.0% | |
| TATATCCCATTCATTATGTC+AGG | + | chr3.3:49549431-49549450 | None:intergenic | 30.0% | |
| TCGGTTTCTAATAGTATCTT+CGG | - | chr3.3:49549266-49549285 | MS.gene08086:CDS | 30.0% | |
| TGATCCATTCATATTTGAAG+TGG | + | chr3.3:49550242-49550261 | None:intergenic | 30.0% | |
| TTGTCTTATATTCTTGGAAC+CGG | - | chr3.3:49549074-49549093 | MS.gene08086:CDS | 30.0% | |
| TTTGAATATGATGTAGGTGA+AGG | + | chr3.3:49549803-49549822 | None:intergenic | 30.0% | |
| ! | TTTGAAGTGGTAAGATTAGA+AGG | + | chr3.3:49550229-49550248 | None:intergenic | 30.0% |
| !! | CACATGGTATTTTTGAATGT+TGG | - | chr3.3:49549651-49549670 | MS.gene08086:CDS | 30.0% |
| !! | GACGATTTTCTGAAGAAATT+AGG | - | chr3.3:49549326-49549345 | MS.gene08086:CDS | 30.0% |
| !!! | ATGACCCAAAAGTGTTTTTA+AGG | + | chr3.3:49550144-49550163 | None:intergenic | 30.0% |
| AACGAAAACCAACAACTTTG+GGG | - | chr3.3:49550199-49550218 | MS.gene08086:CDS | 35.0% | |
| AGAACGAAAACCAACAACTT+TGG | - | chr3.3:49550197-49550216 | MS.gene08086:CDS | 35.0% | |
| AGATGATCATGATGTTGATG+AGG | - | chr3.3:49548953-49548972 | MS.gene08086:CDS | 35.0% | |
| CCTTATGTGTAAGCTAATGA+TGG | - | chr3.3:49550060-49550079 | MS.gene08086:CDS | 35.0% | |
| CTAATAGTATCTTCGGTTCA+AGG | - | chr3.3:49549273-49549292 | MS.gene08086:CDS | 35.0% | |
| GAAAGCCATTACATCATCTT+TGG | + | chr3.3:49548916-49548935 | None:intergenic | 35.0% | |
| GAACGAAAACCAACAACTTT+GGG | - | chr3.3:49550198-49550217 | MS.gene08086:CDS | 35.0% | |
| GCGATGAACTCAATAAAATG+AGG | - | chr3.3:49550431-49550450 | MS.gene08086:CDS | 35.0% | |
| GGTGTGTTGTCTTATATTCT+TGG | - | chr3.3:49549068-49549087 | MS.gene08086:CDS | 35.0% | |
| TAATCTTTCTTCTCAGTTCG+AGG | + | chr3.3:49549389-49549408 | None:intergenic | 35.0% | |
| TTCAGCCAAAGATGATGTAA+TGG | - | chr3.3:49548908-49548927 | MS.gene08086:CDS | 35.0% | |
| ! | ACCAGTCTAATTTTCCATCA+GGG | + | chr3.3:49550402-49550421 | None:intergenic | 35.0% |
| ! | AGTTTGACAAGGTTTTCCAT+TGG | + | chr3.3:49548817-49548836 | None:intergenic | 35.0% |
| ! | ATCATGATGTTGATGAGGTA+GGG | - | chr3.3:49548958-49548977 | MS.gene08086:CDS | 35.0% |
| ! | CCATCATTAGCTTACACATA+AGG | + | chr3.3:49550063-49550082 | None:intergenic | 35.0% |
| ! | GGTAGTTCAGTTGATATGAA+CGG | - | chr3.3:49549218-49549237 | MS.gene08086:CDS | 35.0% |
| ! | TGATACAACTGCATTGATGT+CGG | + | chr3.3:49550178-49550197 | None:intergenic | 35.0% |
| !! | GCAGTTTTCAATCTGTTTCT+CGG | + | chr3.3:49549995-49550014 | None:intergenic | 35.0% |
| AAGGATAACCCCAAAGTTGT+TGG | + | chr3.3:49550210-49550229 | None:intergenic | 40.0% | |
| AATAAGGCAGCAACCATAAG+AGG | + | chr3.3:49549038-49549057 | None:intergenic | 40.0% | |
| AATGGTTGTTGCATCAAACG+TGG | - | chr3.3:49550498-49550517 | MS.gene08086:CDS | 40.0% | |
| ACTCCTGCAAGCTTATTTGA+TGG | + | chr3.3:49550109-49550128 | None:intergenic | 40.0% | |
| AGGAAGAGGATACTACTAGA+AGG | - | chr3.3:49549293-49549312 | MS.gene08086:CDS | 40.0% | |
| CAGAAGTAGCACTTCAACAA+TGG | - | chr3.3:49550480-49550499 | MS.gene08086:CDS | 40.0% | |
| CCCATTCATTATGTCAGGTA+GGG | + | chr3.3:49549426-49549445 | None:intergenic | 40.0% | |
| CCCTACCTGACATAATGAAT+GGG | - | chr3.3:49549423-49549442 | MS.gene08086:CDS | 40.0% | |
| CTAGGGCTTATTCTCATGAT+TGG | - | chr3.3:49549498-49549517 | MS.gene08086:CDS | 40.0% | |
| GAAATTAGGTTTCCAAACGG+TGG | - | chr3.3:49549340-49549359 | MS.gene08086:CDS | 40.0% | |
| GAAGAGGATACTACTAGAAG+GGG | - | chr3.3:49549295-49549314 | MS.gene08086:CDS | 40.0% | |
| GATGCTTGTGAATATGCACA+TGG | - | chr3.3:49549635-49549654 | MS.gene08086:CDS | 40.0% | |
| GGAAGAGGATACTACTAGAA+GGG | - | chr3.3:49549294-49549313 | MS.gene08086:CDS | 40.0% | |
| TCCCATTCATTATGTCAGGT+AGG | + | chr3.3:49549427-49549446 | None:intergenic | 40.0% | |
| TCCCTACCTGACATAATGAA+TGG | - | chr3.3:49549422-49549441 | MS.gene08086:CDS | 40.0% | |
| TCGGTTAACATCGACATAAC+CGG | + | chr3.3:49549096-49549115 | None:intergenic | 40.0% | |
| TCTCCATCAAATAAGCTTGC+AGG | - | chr3.3:49550103-49550122 | MS.gene08086:CDS | 40.0% | |
| TGAGAATAAGCCCTAGAACA+AGG | + | chr3.3:49549494-49549513 | None:intergenic | 40.0% | |
| TTATGGTATGGAAGGAGGAT+TGG | - | chr3.3:49548981-49549000 | MS.gene08086:CDS | 40.0% | |
| TTCATTATGTCAGGTAGGGA+TGG | + | chr3.3:49549422-49549441 | None:intergenic | 40.0% | |
| ! | AAATTAGACTGGTCCATCTG+CGG | - | chr3.3:49550409-49550428 | MS.gene08086:CDS | 40.0% |
| ! | ATCTGTTTCTCGGCATTTGA+AGG | + | chr3.3:49549985-49550004 | None:intergenic | 40.0% |
| ! | ATGTTGATGAGGTAGGGTTA+TGG | - | chr3.3:49548964-49548983 | MS.gene08086:CDS | 40.0% |
| ! | CAGTTGATATGAACGGTAAC+CGG | - | chr3.3:49549225-49549244 | MS.gene08086:CDS | 40.0% |
| ! | CCAGTCTAATTTTCCATCAG+GGG | + | chr3.3:49550401-49550420 | None:intergenic | 40.0% |
| ! | CTAATGATGGAAGAGAAGAC+AGG | - | chr3.3:49550073-49550092 | MS.gene08086:CDS | 40.0% |
| ! | CTTATGGTTGCTGCCTTATT+TGG | - | chr3.3:49549038-49549057 | MS.gene08086:CDS | 40.0% |
| ! | GACCAGTCTAATTTTCCATC+AGG | + | chr3.3:49550403-49550422 | None:intergenic | 40.0% |
| ! | GATCATGATGTTGATGAGGT+AGG | - | chr3.3:49548957-49548976 | MS.gene08086:CDS | 40.0% |
| ! | TCTGTTTCTCGGCATTTGAA+GGG | + | chr3.3:49549984-49550003 | None:intergenic | 40.0% |
| !! | CAAAGTGAAGCCTTGTTCTA+GGG | - | chr3.3:49549481-49549500 | MS.gene08086:CDS | 40.0% |
| AGACAATCAAGTCGCTACAC+CGG | + | chr3.3:49549247-49549266 | None:intergenic | 45.0% | |
| AGCAAAGAAGCAAACCCTTC+TGG | + | chr3.3:49549729-49549748 | None:intergenic | 45.0% | |
| AGCAGCAGAACAACCTGAAA+CGG | + | chr3.3:49549156-49549175 | None:intergenic | 45.0% | |
| AGGAAATGGCATGTGAAGAG+AGG | - | chr3.3:49549009-49549028 | MS.gene08086:CDS | 45.0% | |
| AGGTAGGGTTATGGTATGGA+AGG | - | chr3.3:49548973-49548992 | MS.gene08086:CDS | 45.0% | |
| ATACCGAACAAGGCTTTGCA+AGG | - | chr3.3:49549688-49549707 | MS.gene08086:CDS | 45.0% | |
| ATCCTGCTCAATACCGAACA+AGG | - | chr3.3:49549678-49549697 | MS.gene08086:CDS | 45.0% | |
| ATGGCAACTAAGGTAGCAGA+AGG | + | chr3.3:49550349-49550368 | None:intergenic | 45.0% | |
| CACACCCTTGCTTCCAAATA+AGG | + | chr3.3:49549054-49549073 | None:intergenic | 45.0% | |
| CCCCTGATGGAAAATTAGAC+TGG | - | chr3.3:49550398-49550417 | MS.gene08086:CDS | 45.0% | |
| CTTCTTTGCTCACAAACCAG+AGG | - | chr3.3:49549739-49549758 | MS.gene08086:CDS | 45.0% | |
| GATGAGGTAGGGTTATGGTA+TGG | - | chr3.3:49548969-49548988 | MS.gene08086:CDS | 45.0% | |
| GTATCTTCGGTTCAAGGAAG+AGG | - | chr3.3:49549279-49549298 | MS.gene08086:CDS | 45.0% | |
| GTGTAGCGACTTGATTGTCT+CGG | - | chr3.3:49549247-49549266 | MS.gene08086:CDS | 45.0% | |
| TAAGGTAGCAGAAGGAAGCT+CGG | + | chr3.3:49550341-49550360 | None:intergenic | 45.0% | |
| TAGGGTTATGGTATGGAAGG+AGG | - | chr3.3:49548976-49548995 | MS.gene08086:CDS | 45.0% | |
| TGAAGAGAGGACACCTCTTA+TGG | - | chr3.3:49549022-49549041 | MS.gene08086:CDS | 45.0% | |
| TTCACCTAGCGCTTCTTCAA+TGG | - | chr3.3:49549823-49549842 | MS.gene08086:CDS | 45.0% | |
| TTCTCGGCATTTGAAGGGTT+GGG | + | chr3.3:49549979-49549998 | None:intergenic | 45.0% | |
| TTCTGCGCTCAATAAAGCTC+TGG | + | chr3.3:49550300-49550319 | None:intergenic | 45.0% | |
| TTCTGCTACCTTAGTTGCCA+TGG | - | chr3.3:49550348-49550367 | MS.gene08086:CDS | 45.0% | |
| TTGCTTGATGCATCTGCAGA+TGG | - | chr3.3:49549197-49549216 | MS.gene08086:CDS | 45.0% | |
| TTTCTCGGCATTTGAAGGGT+TGG | + | chr3.3:49549980-49549999 | None:intergenic | 45.0% | |
| ! | GAACCGGTAGAAGCATACAA+CGG | + | chr3.3:49549773-49549792 | None:intergenic | 45.0% |
| !! | CTTCCTTGCAAAGCCTTGTT+CGG | + | chr3.3:49549694-49549713 | None:intergenic | 45.0% |
| !! | GAATCCATTGAAGAAGCGCT+AGG | + | chr3.3:49549830-49549849 | None:intergenic | 45.0% |
| !! | GAGGATTGGCTCAAAGGAAA+TGG | - | chr3.3:49548995-49549014 | MS.gene08086:CDS | 45.0% |
| !! | GCAAAGTGAAGCCTTGTTCT+AGG | - | chr3.3:49549480-49549499 | MS.gene08086:CDS | 45.0% |
| !! | GCTGCCTTATTTGGAAGCAA+GGG | - | chr3.3:49549047-49549066 | MS.gene08086:CDS | 45.0% |
| !! | TGCTGCCTTATTTGGAAGCA+AGG | - | chr3.3:49549046-49549065 | MS.gene08086:CDS | 45.0% |
| AGAAGGCTCCATGGCAACTA+AGG | + | chr3.3:49550359-49550378 | None:intergenic | 50.0% | |
| AGAGAAGGTAGAAGGCTCCA+TGG | + | chr3.3:49550368-49550387 | None:intergenic | 50.0% | |
| AGCCTTCTACCTTCTCTGGT+TGG | - | chr3.3:49550371-49550390 | MS.gene08086:CDS | 50.0% | |
| AGGCTTTGCAAGGAAGGAAC+CGG | - | chr3.3:49549698-49549717 | MS.gene08086:CDS | 50.0% | |
| AGGTGAAGGCAAAGCAGAAC+CGG | + | chr3.3:49549789-49549808 | None:intergenic | 50.0% | |
| AGTGTCCATTTGTTCACCCG+GGG | - | chr3.3:49549525-49549544 | MS.gene08086:CDS | 50.0% | |
| ATGGAGCCTTCTACCTTCTC+TGG | - | chr3.3:49550367-49550386 | MS.gene08086:CDS | 50.0% | |
| ATTGAGTTCATCGCCGCAGA+TGG | + | chr3.3:49550425-49550444 | None:intergenic | 50.0% | |
| CCTTCTACCTTCTCTGGTTG+GGG | - | chr3.3:49550373-49550392 | MS.gene08086:CDS | 50.0% | |
| CGAACAAGGCTTTGCAAGGA+AGG | - | chr3.3:49549692-49549711 | MS.gene08086:CDS | 50.0% | |
| CGACCGTTGTATGCTTCTAC+CGG | - | chr3.3:49549767-49549786 | MS.gene08086:CDS | 50.0% | |
| CGAGTGTCCATTTGTTCACC+CGG | - | chr3.3:49549523-49549542 | MS.gene08086:CDS | 50.0% | |
| CGGGTGAACAAATGGACACT+CGG | + | chr3.3:49549525-49549544 | None:intergenic | 50.0% | |
| CTGAATCTGAGTCTGCCACA+TGG | + | chr3.3:49549942-49549961 | None:intergenic | 50.0% | |
| GAGTGTCCATTTGTTCACCC+GGG | - | chr3.3:49549524-49549543 | MS.gene08086:CDS | 50.0% | |
| GATGCTGCAGATGGAGTCAA+AGG | + | chr3.3:49549902-49549921 | None:intergenic | 50.0% | |
| GATGGAGTCAAAGGTGGTGT+CGG | + | chr3.3:49549893-49549912 | None:intergenic | 50.0% | |
| GCCTTCTACCTTCTCTGGTT+GGG | - | chr3.3:49550372-49550391 | MS.gene08086:CDS | 50.0% | |
| GTCGATGTTAACCGAGTGTG+CGG | - | chr3.3:49549101-49549120 | MS.gene08086:CDS | 50.0% | |
| GTGGAGCAGCAACAAGATGT+AGG | - | chr3.3:49549359-49549378 | MS.gene08086:CDS | 50.0% | |
| ! | TTGTTCTGCTGCTTCTGCTG+AGG | - | chr3.3:49549163-49549182 | MS.gene08086:CDS | 50.0% |
| ! | TTTTCCCCCGGGTGAACAAA+TGG | + | chr3.3:49549533-49549552 | None:intergenic | 50.0% |
| !! | AGCCTTGTTCGGTATTGAGC+AGG | + | chr3.3:49549683-49549702 | None:intergenic | 50.0% |
| !! | GCTCTTCATTGTGCCGTTTC+AGG | - | chr3.3:49549140-49549159 | MS.gene08086:CDS | 50.0% |
| !! | TGGAAGGAGGATTGGCTCAA+AGG | - | chr3.3:49548989-49549008 | MS.gene08086:CDS | 50.0% |
| AAGGAACCGGATGCACCAGA+AGG | - | chr3.3:49549711-49549730 | MS.gene08086:CDS | 55.0% | |
| ACGACCCTTTCCGAAACTCG+GGG | + | chr3.3:49549604-49549623 | None:intergenic | 55.0% | |
| AGGAACCGGATGCACCAGAA+GGG | - | chr3.3:49549712-49549731 | MS.gene08086:CDS | 55.0% | |
| CACGACCCTTTCCGAAACTC+GGG | + | chr3.3:49549605-49549624 | None:intergenic | 55.0% | |
| CCCCAACCAGAGAAGGTAGA+AGG | + | chr3.3:49550376-49550395 | None:intergenic | 55.0% | |
| CTTGCCCCGAGTTTCGGAAA+GGG | - | chr3.3:49549597-49549616 | MS.gene08086:CDS | 55.0% | |
| CTTGTTGCTGCTCCACCGTT+TGG | + | chr3.3:49549355-49549374 | None:intergenic | 55.0% | |
| GCAAACCCTTCTGGTGCATC+CGG | + | chr3.3:49549720-49549739 | None:intergenic | 55.0% | |
| GCACGACCCTTTCCGAAACT+CGG | + | chr3.3:49549606-49549625 | None:intergenic | 55.0% | |
| GCATGCGATGATGCTGCAGA+TGG | + | chr3.3:49549911-49549930 | None:intergenic | 55.0% | |
| GCTGCAGATGGAGTCAAAGG+TGG | + | chr3.3:49549899-49549918 | None:intergenic | 55.0% | |
| GTCTACCTTGCCCCGAGTTT+CGG | - | chr3.3:49549591-49549610 | MS.gene08086:CDS | 55.0% | |
| GTGTCCATTTGTTCACCCGG+GGG | - | chr3.3:49549526-49549545 | MS.gene08086:CDS | 55.0% | |
| TACAACGGTCGAAGCTCCTC+TGG | + | chr3.3:49549758-49549777 | None:intergenic | 55.0% | |
| ! | CGACGACTTGCATTTTCCCC+CGG | + | chr3.3:49549545-49549564 | None:intergenic | 55.0% |
| ! | GACGACTTGCATTTTCCCCC+GGG | + | chr3.3:49549544-49549563 | None:intergenic | 55.0% |
| ACCGAGTGTGCGGCTCAGAT+AGG | - | chr3.3:49549111-49549130 | MS.gene08086:CDS | 60.0% | |
| AGGGGATCCCCAACCAGAGA+AGG | + | chr3.3:49550383-49550402 | None:intergenic | 60.0% | |
| CCCTATCTGAGCCGCACACT+CGG | + | chr3.3:49549115-49549134 | None:intergenic | 60.0% | |
| CCGAGTGTGCGGCTCAGATA+GGG | - | chr3.3:49549112-49549131 | MS.gene08086:CDS | 60.0% | |
| CCTTGCCCCGAGTTTCGGAA+AGG | - | chr3.3:49549596-49549615 | MS.gene08086:CDS | 60.0% | |
| CCTTTCCGAAACTCGGGGCA+AGG | + | chr3.3:49549599-49549618 | None:intergenic | 60.0% | |
| CGCATGCAGCTGGAACCATG+TGG | - | chr3.3:49549924-49549943 | MS.gene08086:CDS | 60.0% | |
| GCAGCATCATCGCATGCAGC+TGG | - | chr3.3:49549914-49549933 | MS.gene08086:CDS | 60.0% | |
| TCTGGTTGGGGATCCCCTGA+TGG | - | chr3.3:49550385-49550404 | MS.gene08086:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 49548801 | 49550549 | 49548801 | ID=MS.gene08086 |
| chr3.3 | mRNA | 49548801 | 49550549 | 49548801 | ID=MS.gene08086.t1;Parent=MS.gene08086 |
| chr3.3 | exon | 49548801 | 49550549 | 49548801 | ID=MS.gene08086.t1.exon1;Parent=MS.gene08086.t1 |
| chr3.3 | CDS | 49548801 | 49550549 | 49548801 | ID=cds.MS.gene08086.t1;Parent=MS.gene08086.t1 |
| Gene Sequence |
| Protein sequence |
