AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene08224

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08224.t1 MTR_7g085410 95.539 269 4 2 1 269 1 261 0.0 513
MS.gene08224.t1 MTR_6g032830 61.733 277 76 10 1 264 1 260 2.82e-104 304
MS.gene08224.t1 MTR_3g109760 39.200 250 95 9 15 260 75 271 1.60e-35 129
MS.gene08224.t1 MTR_1g094930 76.471 68 16 0 193 260 144 211 2.13e-34 124
MS.gene08224.t1 MTR_1g094930 76.471 68 16 0 193 260 239 306 6.81e-34 125
MS.gene08224.t1 MTR_4g071590 80.000 65 13 0 196 260 211 275 3.99e-33 122
MS.gene08224.t1 MTR_4g471590 80.000 65 13 0 196 260 211 275 3.99e-33 122
MS.gene08224.t1 MTR_2g094600 61.702 94 35 1 172 264 191 284 4.07e-33 123
MS.gene08224.t1 MTR_1g006650 35.985 264 114 9 11 260 76 298 1.07e-32 122
MS.gene08224.t1 MTR_8g076340 81.538 65 12 0 196 260 218 282 2.14e-32 120
MS.gene08224.t1 MTR_5g021340 41.921 229 93 9 45 260 163 364 2.71e-32 122
MS.gene08224.t1 MTR_8g013950 37.118 229 78 7 45 260 115 290 3.78e-32 121
MS.gene08224.t1 MTR_1g046680 74.648 71 18 0 190 260 166 236 7.38e-32 118
MS.gene08224.t1 MTR_1g116010 75.758 66 16 0 196 261 164 229 1.26e-31 117
MS.gene08224.t1 MTR_3g117380 76.923 65 15 0 196 260 229 293 4.58e-31 117
MS.gene08224.t1 MTR_4g058910 71.831 71 20 0 193 263 311 381 2.76e-30 117
MS.gene08224.t1 MTR_5g007600 73.846 65 17 0 196 260 259 323 2.19e-29 113
MS.gene08224.t1 MTR_5g007600 71.875 64 18 0 196 259 157 220 1.04e-27 109
MS.gene08224.t1 MTR_7g446110 58.065 62 26 0 196 257 187 248 7.24e-20 86.7
MS.gene08224.t1 MTR_7g445950 56.923 65 28 0 193 257 117 181 1.40e-19 84.3
MS.gene08224.t1 MTR_7g446070 40.000 105 63 0 153 257 89 193 3.95e-19 83.2
MS.gene08224.t1 MTR_7g445980 57.143 63 26 1 196 257 33 95 1.07e-17 76.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08224.t1 AT4G32890 42.793 222 81 6 45 260 80 261 8.09e-44 151
MS.gene08224.t1 AT2G45050 39.167 240 93 8 21 260 57 243 1.09e-39 139
MS.gene08224.t1 AT5G66320 40.816 245 112 8 28 260 90 313 4.25e-36 131
MS.gene08224.t1 AT5G66320 40.816 245 112 8 28 260 90 313 4.25e-36 131
MS.gene08224.t1 AT3G60530 39.631 217 59 8 45 260 77 222 9.35e-36 128
MS.gene08224.t1 AT5G25830 38.235 238 89 8 45 260 82 283 2.68e-35 129
MS.gene08224.t1 AT3G24050 75.000 68 17 0 193 260 191 258 4.56e-33 122
MS.gene08224.t1 AT4G36240 38.288 222 74 6 45 265 74 233 4.69e-33 121
MS.gene08224.t1 AT3G54810 77.612 67 15 0 194 260 227 293 1.65e-32 122
MS.gene08224.t1 AT3G54810 77.612 67 15 0 194 260 227 293 1.65e-32 122
MS.gene08224.t1 AT3G51080 81.538 65 12 0 196 260 221 285 5.26e-32 120
MS.gene08224.t1 AT1G08010 72.308 65 18 0 196 260 220 284 3.56e-31 117
MS.gene08224.t1 AT1G08010 72.308 65 18 0 196 260 220 284 3.56e-31 117
MS.gene08224.t1 AT1G08010 72.308 65 18 0 196 260 220 284 3.56e-31 117
MS.gene08224.t1 AT1G08000 73.846 65 17 0 196 260 218 282 7.83e-30 114
MS.gene08224.t1 AT1G08000 73.846 65 17 0 196 260 218 282 7.83e-30 114
MS.gene08224.t1 AT4G34680 72.308 65 18 0 196 260 180 244 9.00e-30 113
MS.gene08224.t1 AT4G34680 72.308 65 18 0 196 260 180 244 9.00e-30 113
MS.gene08224.t1 AT2G28340 67.188 64 21 0 198 261 193 256 2.72e-26 104
MS.gene08224.t1 AT3G45170 54.878 82 37 0 185 266 104 185 2.99e-26 102

Find 51 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTCTTAAAACAAGCTTAT+TGG 0.124697 7.3:+23140626 MS.gene08224:CDS
CACTAGAAACTTGTACATTT+TGG 0.144962 7.3:-23139904 None:intergenic
CATGCATTGCATAGTGTCTT+TGG 0.243410 7.3:-23140859 None:intergenic
TGAAAAGGAAATTGTGAAAA+AGG 0.260278 7.3:+23140714 MS.gene08224:CDS
CATAGTGTCTTTGGGCCTAA+TGG 0.278012 7.3:-23140850 None:intergenic
ACACTACTCACTAGGATTAA+TGG 0.294145 7.3:-23141010 None:intergenic
ATGGGCCTGCCCTCCATTGT+GGG 0.318681 7.3:-23140831 None:intergenic
CAATGGAGGGCAGGCCCATT+AGG 0.322478 7.3:+23140835 MS.gene08224:CDS
AAGATGCTTAAAGGGTCTTT+TGG 0.328925 7.3:-23140556 None:intergenic
GTATTGGATAAGATGCTTAA+AGG 0.338824 7.3:-23140565 None:intergenic
ATAGTGTCTTTGGGCCTAAT+GGG 0.339035 7.3:-23140849 None:intergenic
GGTTTCTTCACTGTGTTTGA+AGG 0.343748 7.3:-23140451 None:intergenic
GTACACCTCCTTGTTGGAAT+TGG 0.356244 7.3:-23140787 None:intergenic
ATGCATTGCATAGTGTCTTT+GGG 0.368727 7.3:-23140858 None:intergenic
AATGGGCCTGCCCTCCATTG+TGG 0.406749 7.3:-23140832 None:intergenic
TATTGGATAAGATGCTTAAA+GGG 0.429212 7.3:-23140564 None:intergenic
CAATGTGTACACCTCCTTGT+TGG 0.437336 7.3:-23140793 None:intergenic
AAATTCACACAAGAAAGTTA+TGG 0.446713 7.3:+23140963 MS.gene08224:CDS
AGATTATGAAGTGGTTGAAA+AGG 0.475929 7.3:+23140699 MS.gene08224:CDS
GTAAAAGTACACAACTTTGT+GGG 0.484296 7.3:-23140387 None:intergenic
TAGTGAACTCATGGTACCTA+AGG 0.486078 7.3:+23140657 MS.gene08224:CDS
GGTAAAAGTACACAACTTTG+TGG 0.499157 7.3:-23140388 None:intergenic
CAGGCAAAGCAAGAAGCAAA+AGG 0.500793 7.3:+23140509 MS.gene08224:CDS
AGGACCCCACAATGGAGGGC+AGG 0.512119 7.3:+23140826 MS.gene08224:CDS
ATTATGTAGGAAGTGGATGT+TGG 0.517271 7.3:+23140322 MS.gene08224:intron
TTGAAAGGTTAGAGCAAGAA+GGG 0.531774 7.3:-23139952 None:intergenic
CTTGAAAGGTTAGAGCAAGA+AGG 0.532182 7.3:-23139953 None:intergenic
ACAAAAGTTGGACTCTTAGC+AGG 0.532481 7.3:-23140925 None:intergenic
TAGTGAGTGCAAACAAGAGA+AGG 0.541293 7.3:+23139922 MS.gene08224:CDS
GTTAGCTGATAGTGAACTCA+TGG 0.566356 7.3:+23140648 MS.gene08224:CDS
TGGGCCTGCCCTCCATTGTG+GGG 0.567754 7.3:-23140830 None:intergenic
GGTCATCAAGGCTACTTGAA+AGG 0.569542 7.3:-23139967 None:intergenic
GAGCAGAAAAGAGGTCATCA+AGG 0.570986 7.3:-23139979 None:intergenic
TGTGGTGTGAGGTACAAATC+TGG 0.576464 7.3:+23140880 MS.gene08224:CDS
GTAGGAAGTGGATGTTGGAA+TGG 0.583317 7.3:+23140327 MS.gene08224:intron
TTTGAGGTTGATGTTAACAA+TGG 0.593552 7.3:+23140757 MS.gene08224:CDS
ACAAAGGACCCCACAATGGA+GGG 0.595325 7.3:+23140822 MS.gene08224:CDS
CTCTTAGCAGGTCTATACTC+AGG 0.605156 7.3:-23140913 None:intergenic
CACAAAGGACCCCACAATGG+AGG 0.605327 7.3:+23140821 MS.gene08224:CDS
ACAAGAAAGTTATGGAGATG+AGG 0.611218 7.3:+23140971 MS.gene08224:CDS
GGTAACAAAAGATTATGAAG+TGG 0.619135 7.3:+23140690 MS.gene08224:CDS
AGTGAACTCATGGTACCTAA+GGG 0.625585 7.3:+23140658 MS.gene08224:CDS
AACCACTAACACTACTCACT+AGG 0.631055 7.3:-23141018 None:intergenic
AAGTGGATGTTGGAATGGAA+TGG 0.634243 7.3:+23140332 MS.gene08224:CDS
GTCAAAATCCAATTCCAACA+AGG 0.638845 7.3:+23140779 MS.gene08224:CDS
GTACACATTGTTTGTCACAA+AGG 0.655851 7.3:+23140806 MS.gene08224:CDS
GTGTGAGGTACAAATCTGGT+AGG 0.664094 7.3:+23140884 MS.gene08224:CDS
TGTCACAAAGGACCCCACAA+TGG 0.681006 7.3:+23140818 MS.gene08224:CDS
AAAATCCAATTCCAACAAGG+AGG 0.704851 7.3:+23140782 MS.gene08224:CDS
TATGCAATGCATGTGGTGTG+AGG 0.734829 7.3:+23140869 MS.gene08224:CDS
AAGACACTATGCAATGCATG+TGG 0.751831 7.3:+23140862 MS.gene08224:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTAGAAACATAATATTAA+AGG - chr7.3:23140219-23140238 None:intergenic 10.0%
!!! TATTATGTTTCTAATTTTCT+TGG + chr7.3:23140223-23140242 MS.gene08224:intron 15.0%
!!! TGTAGTTTTTGCTAAAATTA+TGG + chr7.3:23140267-23140286 MS.gene08224:intron 20.0%
! AAATTCACACAAGAAAGTTA+TGG + chr7.3:23140963-23140982 MS.gene08224:CDS 25.0%
! GAAAAACATATGTTAGAACA+AGG - chr7.3:23140134-23140153 None:intergenic 25.0%
! TATTGGATAAGATGCTTAAA+GGG - chr7.3:23140567-23140586 None:intergenic 25.0%
! TGAAAAGGAAATTGTGAAAA+AGG + chr7.3:23140714-23140733 MS.gene08224:CDS 25.0%
! TTTGACCAAACAAATTATGT+AGG + chr7.3:23140309-23140328 MS.gene08224:intron 25.0%
AGATTATGAAGTGGTTGAAA+AGG + chr7.3:23140699-23140718 MS.gene08224:CDS 30.0%
AGGAAATTGTGAAAAAGGAA+AGG + chr7.3:23140719-23140738 MS.gene08224:CDS 30.0%
CAAACAAATTATGTAGGAAG+TGG + chr7.3:23140315-23140334 MS.gene08224:intron 30.0%
CTCTCTTAAAACAAGCTTAT+TGG + chr7.3:23140626-23140645 MS.gene08224:CDS 30.0%
GGTAACAAAAGATTATGAAG+TGG + chr7.3:23140690-23140709 MS.gene08224:CDS 30.0%
GTATTGGATAAGATGCTTAA+AGG - chr7.3:23140568-23140587 None:intergenic 30.0%
TGCAAAAAACTCACAAAAGT+TGG - chr7.3:23140940-23140959 None:intergenic 30.0%
! AACATAGTGACTCATTTTGA+GGG - chr7.3:23140597-23140616 None:intergenic 30.0%
! CACTAGAAACTTGTACATTT+TGG - chr7.3:23139907-23139926 None:intergenic 30.0%
! GTAAAAGTACACAACTTTGT+GGG - chr7.3:23140390-23140409 None:intergenic 30.0%
!! AAAGGAAAGGTTTGATTTTG+AGG + chr7.3:23140732-23140751 MS.gene08224:CDS 30.0%
!! AAGGAAAGGTTTGATTTTGA+GGG + chr7.3:23140733-23140752 MS.gene08224:CDS 30.0%
!! ATTTTGAGGGTTTAAGGTAT+TGG - chr7.3:23140584-23140603 None:intergenic 30.0%
!! CACTTCCTACATAATTTGTT+TGG - chr7.3:23140317-23140336 None:intergenic 30.0%
!! GACTCATTTTGTTCTTGTTT+TGG - chr7.3:23140475-23140494 None:intergenic 30.0%
!! GTTGTGTTTTGAACATTAGA+GGG - chr7.3:23140412-23140431 None:intergenic 30.0%
!! TGTTGTGTTTTGAACATTAG+AGG - chr7.3:23140413-23140432 None:intergenic 30.0%
!! TTTGAGGTTGATGTTAACAA+TGG + chr7.3:23140757-23140776 MS.gene08224:CDS 30.0%
!!! CAATAAGCTTGTTTTAAGAG+AGG - chr7.3:23140628-23140647 None:intergenic 30.0%
AAAATCCAATTCCAACAAGG+AGG + chr7.3:23140782-23140801 MS.gene08224:CDS 35.0%
ACAAGAAAGTTATGGAGATG+AGG + chr7.3:23140971-23140990 MS.gene08224:CDS 35.0%
ATGCATTGCATAGTGTCTTT+GGG - chr7.3:23140861-23140880 None:intergenic 35.0%
ATTATGTAGGAAGTGGATGT+TGG + chr7.3:23140322-23140341 MS.gene08224:intron 35.0%
CATAACTAGGAAGGACTTAT+AGG - chr7.3:23140110-23140129 None:intergenic 35.0%
GTACACATTGTTTGTCACAA+AGG + chr7.3:23140806-23140825 MS.gene08224:CDS 35.0%
GTCAAAATCCAATTCCAACA+AGG + chr7.3:23140779-23140798 MS.gene08224:CDS 35.0%
GTTAGAACAAGGACATAACT+AGG - chr7.3:23140123-23140142 None:intergenic 35.0%
TTGAAAGGTTAGAGCAAGAA+GGG - chr7.3:23139955-23139974 None:intergenic 35.0%
! AAGATGCTTAAAGGGTCTTT+TGG - chr7.3:23140559-23140578 None:intergenic 35.0%
! GAACATAGTGACTCATTTTG+AGG - chr7.3:23140598-23140617 None:intergenic 35.0%
! GGTAAAAGTACACAACTTTG+TGG - chr7.3:23140391-23140410 None:intergenic 35.0%
!! AGGAAAGGTTTGATTTTGAG+GGG + chr7.3:23140734-23140753 MS.gene08224:CDS 35.0%
!! TGACTCATTTTGAGGGTTTA+AGG - chr7.3:23140590-23140609 None:intergenic 35.0%
!!! AAGCTTGTTTTAAGAGAGGT+GGG - chr7.3:23140624-23140643 None:intergenic 35.0%
!!! TAAGCTTGTTTTAAGAGAGG+TGG - chr7.3:23140625-23140644 None:intergenic 35.0%
AAGACACTATGCAATGCATG+TGG + chr7.3:23140862-23140881 MS.gene08224:CDS 40.0%
AAGTGGATGTTGGAATGGAA+TGG + chr7.3:23140332-23140351 MS.gene08224:CDS 40.0%
ACAAAAGTTGGACTCTTAGC+AGG - chr7.3:23140928-23140947 None:intergenic 40.0%
AGTGAACTCATGGTACCTAA+GGG + chr7.3:23140658-23140677 MS.gene08224:CDS 40.0%
ATAGTGTCTTTGGGCCTAAT+GGG - chr7.3:23140852-23140871 None:intergenic 40.0%
CATGCATTGCATAGTGTCTT+TGG - chr7.3:23140862-23140881 None:intergenic 40.0%
CTTGAAAGGTTAGAGCAAGA+AGG - chr7.3:23139956-23139975 None:intergenic 40.0%
GAACAAGGACATAACTAGGA+AGG - chr7.3:23140119-23140138 None:intergenic 40.0%
GGTTTCTTCACTGTGTTTGA+AGG - chr7.3:23140454-23140473 None:intergenic 40.0%
TAGTGAACTCATGGTACCTA+AGG + chr7.3:23140657-23140676 MS.gene08224:CDS 40.0%
TAGTGAGTGCAAACAAGAGA+AGG + chr7.3:23139922-23139941 MS.gene08224:CDS 40.0%
! CCTCTTTTCTGCTCAAAACA+CGG + chr7.3:23139988-23140007 MS.gene08224:CDS 40.0%
! GTTAGCTGATAGTGAACTCA+TGG + chr7.3:23140648-23140667 MS.gene08224:CDS 40.0%
!! AAAGGGTCTTTTGGTCTTCT+TGG - chr7.3:23140550-23140569 None:intergenic 40.0%
!! GTTACCTCTTTTTCACCCTT+AGG - chr7.3:23140676-23140695 None:intergenic 40.0%
!! GTTTGATTTTGAGGGGTTTG+AGG + chr7.3:23140741-23140760 MS.gene08224:CDS 40.0%
CAATGTGTACACCTCCTTGT+TGG - chr7.3:23140796-23140815 None:intergenic 45.0%
CAGGCAAAGCAAGAAGCAAA+AGG + chr7.3:23140509-23140528 MS.gene08224:CDS 45.0%
CATAGTGTCTTTGGGCCTAA+TGG - chr7.3:23140853-23140872 None:intergenic 45.0%
CTCTTAGCAGGTCTATACTC+AGG - chr7.3:23140916-23140935 None:intergenic 45.0%
GAGCAGAAAAGAGGTCATCA+AGG - chr7.3:23139982-23140001 None:intergenic 45.0%
GGTACCTAAGGGTGAAAAAG+AGG + chr7.3:23140669-23140688 MS.gene08224:CDS 45.0%
GGTCATCAAGGCTACTTGAA+AGG - chr7.3:23139970-23139989 None:intergenic 45.0%
GTACACCTCCTTGTTGGAAT+TGG - chr7.3:23140790-23140809 None:intergenic 45.0%
GTAGGAAGTGGATGTTGGAA+TGG + chr7.3:23140327-23140346 MS.gene08224:intron 45.0%
GTGTGAGGTACAAATCTGGT+AGG + chr7.3:23140884-23140903 MS.gene08224:CDS 45.0%
TATGCAATGCATGTGGTGTG+AGG + chr7.3:23140869-23140888 MS.gene08224:CDS 45.0%
TGTGGTGTGAGGTACAAATC+TGG + chr7.3:23140880-23140899 MS.gene08224:CDS 45.0%
! CTTTTGCTTCTTGCTTTGCC+TGG - chr7.3:23140511-23140530 None:intergenic 45.0%
! GAGTCAACTTTTGCAGTTCC+AGG + chr7.3:23140490-23140509 MS.gene08224:CDS 45.0%
!! CCGTGTTTTGAGCAGAAAAG+AGG - chr7.3:23139991-23140010 None:intergenic 45.0%
ACAAAGGACCCCACAATGGA+GGG + chr7.3:23140822-23140841 MS.gene08224:CDS 50.0%
TGTCACAAAGGACCCCACAA+TGG + chr7.3:23140818-23140837 MS.gene08224:CDS 50.0%
CACAAAGGACCCCACAATGG+AGG + chr7.3:23140821-23140840 MS.gene08224:CDS 55.0%
AATGGGCCTGCCCTCCATTG+TGG - chr7.3:23140835-23140854 None:intergenic 60.0%
ATGGGCCTGCCCTCCATTGT+GGG - chr7.3:23140834-23140853 None:intergenic 60.0%
CAATGGAGGGCAGGCCCATT+AGG + chr7.3:23140835-23140854 MS.gene08224:CDS 60.0%
AGGACCCCACAATGGAGGGC+AGG + chr7.3:23140826-23140845 MS.gene08224:CDS 65.0%
TGGGCCTGCCCTCCATTGTG+GGG - chr7.3:23140833-23140852 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 23139893 23141029 23139893 ID=MS.gene08224
chr7.3 mRNA 23139893 23141029 23139893 ID=MS.gene08224.t1;Parent=MS.gene08224
chr7.3 exon 23139893 23140009 23139893 ID=MS.gene08224.t1.exon1;Parent=MS.gene08224.t1
chr7.3 CDS 23139893 23140009 23139893 ID=cds.MS.gene08224.t1;Parent=MS.gene08224.t1
chr7.3 exon 23140331 23141029 23140331 ID=MS.gene08224.t1.exon2;Parent=MS.gene08224.t1
chr7.3 CDS 23140331 23141029 23140331 ID=cds.MS.gene08224.t1;Parent=MS.gene08224.t1
Gene Sequence

>MS.gene08224

ATGGATGTGTGCCAAAATGTACAAGTTTCTAGTGAGTGCAAACAAGAGAAGGTTCTTAACCCTTCTTGCTCTAACCTTTCAAGTAGCCTTGATGACCTCTTTTCTGCTCAAAACACGGTTAGTCTTTGTTTCACTTTAATATTAACTTCTTCAAAGTTCACTCTTTTTCTTCTGTTTTTCAACTTTTGTTACTATTCTAGCTTAACTCACTTGTCCTATAAGTCCTTCCTAGTTATGTCCTTGTTCTAACATATGTTTTTCTTACTATGTTTGAAAAAAGTAATTTTTTTTGTTTGACTAAATATATGTTTACAAAGTTTATACCTTTAATATTATGTTTCTAATTTTCTTGGTTTATTTAAGTGTTTCTAAAATGTAGTTTTTGCTAAAATTATGGTTGACTAAATTTTACATATTTTGACCAAACAAATTATGTAGGAAGTGGATGTTGGAATGGAATGGCTTTCAGTGTTTGTTGAAGACTGTTTTTCAAGCCCACAAAGTTGTGTACTTTTACCCTCTAATGTTCAAAACACAACAAGTACTGTCTCATCAAAACCTTCAAACACAGTGAAGAAACCAAAACAAGAACAAAATGAGTCAACTTTTGCAGTTCCAGGCAAAGCAAGAAGCAAAAGGAAGAGACTTTCAGCTCCAAGAAGACCAAAAGACCCTTTAAGCATCTTATCCAATACCTTAAACCCTCAAAATGAGTCACTATGTTCTGACCCACCTCTCTTAAAACAAGCTTATTGGTTAGCTGATAGTGAACTCATGGTACCTAAGGGTGAAAAAGAGGTAACAAAAGATTATGAAGTGGTTGAAAAGGAAATTGTGAAAAAGGAAAGGTTTGATTTTGAGGGGTTTGAGGTTGATGTTAACAATGGTCAAAATCCAATTCCAACAAGGAGGTGTACACATTGTTTGTCACAAAGGACCCCACAATGGAGGGCAGGCCCATTAGGCCCAAAGACACTATGCAATGCATGTGGTGTGAGGTACAAATCTGGTAGGTTGTTGCCTGAGTATAGACCTGCTAAGAGTCCAACTTTTGTGAGTTTTTTGCATTCAAATTCACACAAGAAAGTTATGGAGATGAGGATGAATGTTGTATCTTCCATTAATCCTAGTGAGTAG
Protein sequence

>MS.gene08224.t1

MDVCQNVQVSSECKQEKVLNPSCSNLSSSLDDLFSAQNTEVDVGMEWLSVFVEDCFSSPQSCVLLPSNVQNTTSTVSSKPSNTVKKPKQEQNESTFAVPGKARSKRKRLSAPRRPKDPLSILSNTLNPQNESLCSDPPLLKQAYWLADSELMVPKGEKEVTKDYEVVEKEIVKKERFDFEGFEVDVNNGQNPIPTRRCTHCLSQRTPQWRAGPLGPKTLCNACGVRYKSGRLLPEYRPAKSPTFVSFLHSNSHKKVMEMRMNVVSSINPSE