AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene08239

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08239.t1 MTR_3g072710 97.250 509 9 3 1 505 1 508 0.0 1013
MS.gene08239.t1 MTR_5g058860 62.279 509 154 14 12 505 21 506 0.0 578
MS.gene08239.t1 MTR_8g093070 38.191 398 208 12 116 504 136 504 1.53e-66 223
MS.gene08239.t1 MTR_7g027190 33.607 488 273 15 30 497 218 674 4.95e-65 223
MS.gene08239.t1 MTR_7g074650 37.203 379 201 16 124 495 443 791 1.49e-51 187
MS.gene08239.t1 MTR_3g065980 31.236 445 250 13 76 495 126 539 1.69e-51 184
MS.gene08239.t1 MTR_1g069725 31.100 418 253 12 89 500 79 467 8.18e-49 175
MS.gene08239.t1 MTR_4g076020 36.828 372 193 13 133 498 96 431 2.52e-48 172
MS.gene08239.t1 MTR_3g089055 31.514 403 242 11 104 495 190 569 6.65e-48 174
MS.gene08239.t1 MTR_3g089055 31.514 403 242 11 104 495 185 564 8.18e-48 174
MS.gene08239.t1 MTR_1g029420 32.564 390 229 11 118 495 224 591 2.13e-47 173
MS.gene08239.t1 MTR_4g104020 31.683 404 252 9 103 497 133 521 2.32e-47 172
MS.gene08239.t1 MTR_4g077760 32.880 368 228 8 131 495 203 554 6.44e-46 169
MS.gene08239.t1 MTR_8g442410 31.320 447 265 12 81 504 106 533 2.29e-45 167
MS.gene08239.t1 MTR_5g097480 29.397 398 251 10 107 495 167 543 7.84e-43 160
MS.gene08239.t1 MTR_2g082090 32.126 414 238 15 101 495 189 578 1.62e-42 159
MS.gene08239.t1 MTR_3g056110 30.894 369 221 10 140 495 194 541 5.21e-41 155
MS.gene08239.t1 MTR_5g094450 28.682 387 256 8 116 495 158 531 2.22e-40 153
MS.gene08239.t1 MTR_1g096030 31.206 423 221 16 109 495 70 458 2.42e-39 149
MS.gene08239.t1 MTR_4g102790 30.993 413 222 16 112 496 285 662 2.61e-39 152
MS.gene08239.t1 MTR_4g133660 28.244 393 253 8 114 497 172 544 1.21e-37 145
MS.gene08239.t1 MTR_2g026250 27.171 357 228 11 154 497 260 597 8.82e-35 138
MS.gene08239.t1 MTR_2g034250 26.835 395 271 9 109 495 200 584 2.62e-34 136
MS.gene08239.t1 MTR_2g034260 27.681 401 259 14 109 495 200 583 4.75e-33 132
MS.gene08239.t1 MTR_4g064150 25.238 420 282 11 89 496 334 733 4.36e-32 130
MS.gene08239.t1 MTR_2g034280 26.531 392 273 10 109 495 193 574 1.03e-31 129
MS.gene08239.t1 MTR_4g064200 28.362 409 240 17 111 498 276 652 1.90e-31 128
MS.gene08239.t1 MTR_2g097410 26.329 414 258 14 103 496 355 741 2.94e-31 128
MS.gene08239.t1 MTR_7g062120 27.143 420 263 12 93 495 346 739 5.84e-31 127
MS.gene08239.t1 MTR_2g097467 28.032 371 225 14 144 496 308 654 1.41e-29 123
MS.gene08239.t1 MTR_2g099110 29.430 316 203 9 98 409 53 352 5.83e-29 119
MS.gene08239.t1 MTR_4g097080 27.202 386 258 11 118 495 132 502 6.36e-29 120
MS.gene08239.t1 MTR_4g064120 24.311 399 269 11 66 449 201 581 1.51e-28 119
MS.gene08239.t1 MTR_3g022830 24.554 448 258 13 105 495 11 435 4.60e-28 116
MS.gene08239.t1 MTR_0092s0100 27.949 390 244 15 116 497 369 729 2.53e-27 116
MS.gene08239.t1 MTR_0092s0100 27.949 390 244 15 116 497 372 732 2.73e-27 116
MS.gene08239.t1 MTR_4g064160 25.175 429 280 13 84 496 281 684 3.47e-27 115
MS.gene08239.t1 MTR_2g097463 29.393 313 192 11 200 496 356 655 4.45e-27 115
MS.gene08239.t1 MTR_5g015490 27.273 396 251 14 118 495 113 489 5.66e-26 111
MS.gene08239.t1 MTR_4g064180 24.590 427 284 13 87 497 220 624 9.28e-26 111
MS.gene08239.t1 MTR_2g097473 27.933 358 220 13 154 496 320 654 2.20e-25 110
MS.gene08239.t1 MTR_7g109580 25.377 398 263 14 109 495 179 553 4.53e-25 108
MS.gene08239.t1 MTR_2g097390 24.831 443 280 15 71 495 233 640 1.73e-24 107
MS.gene08239.t1 MTR_2g097310 24.605 443 283 15 71 496 230 638 1.40e-23 104
MS.gene08239.t1 MTR_8g077940 28.267 375 227 13 136 497 197 542 5.84e-23 102
MS.gene08239.t1 MTR_4g026485 25.952 420 264 15 86 497 245 625 6.07e-22 99.8
MS.gene08239.t1 MTR_2g097350 25.823 395 246 15 119 495 274 639 7.73e-22 99.4
MS.gene08239.t1 MTR_2g089100 27.692 325 195 10 210 502 133 449 1.21e-21 97.8
MS.gene08239.t1 MTR_4g076140 30.043 233 142 7 105 335 34 247 2.88e-21 97.1
MS.gene08239.t1 MTR_4g076140 28.000 150 102 3 349 495 322 468 1.30e-12 70.1
MS.gene08239.t1 MTR_5g009080 28.936 235 144 8 104 335 41 255 6.35e-21 95.9
MS.gene08239.t1 MTR_5g009080 31.646 158 94 5 345 495 327 477 2.27e-13 72.8
MS.gene08239.t1 MTR_2g097380 24.528 371 202 14 148 496 247 561 1.49e-20 95.1
MS.gene08239.t1 MTR_7g069740 26.161 409 253 14 105 495 206 583 1.91e-20 95.1
MS.gene08239.t1 MTR_1g086970 23.500 400 275 12 109 495 96 477 3.26e-19 90.9
MS.gene08239.t1 MTR_8g020840 24.747 396 262 12 118 495 170 547 1.48e-15 79.7
MS.gene08239.t1 MTR_4g095500 23.488 430 257 16 118 495 58 467 7.00e-13 71.2
MS.gene08239.t1 MTR_5g015950 21.564 422 277 10 118 495 34 445 8.24e-13 70.9
MS.gene08239.t1 MTR_3g053270 21.564 422 277 10 118 495 34 445 8.24e-13 70.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08239.t1 AT4G08250 51.098 501 206 13 11 500 10 482 2.92e-158 459
MS.gene08239.t1 AT3G54220 36.386 415 229 16 94 499 265 653 1.11e-51 186
MS.gene08239.t1 AT5G41920 35.264 397 230 12 102 495 27 399 4.08e-51 179
MS.gene08239.t1 AT1G66350 32.609 414 232 15 90 495 131 505 1.18e-47 172
MS.gene08239.t1 AT5G66770 32.274 409 254 7 93 495 192 583 2.43e-47 173
MS.gene08239.t1 AT3G03450 31.266 403 234 14 107 495 171 544 1.51e-45 167
MS.gene08239.t1 AT3G50650 33.699 365 214 7 141 495 195 541 1.66e-45 167
MS.gene08239.t1 AT5G17490 32.688 413 236 14 98 499 138 519 1.67e-45 167
MS.gene08239.t1 AT1G55580 34.505 313 188 7 196 495 136 444 1.35e-43 160
MS.gene08239.t1 AT5G48150 29.639 388 240 10 120 495 123 489 1.13e-42 159
MS.gene08239.t1 AT5G48150 29.639 388 240 10 120 495 123 489 1.13e-42 159
MS.gene08239.t1 AT5G48150 29.639 388 240 10 120 495 123 489 1.13e-42 159
MS.gene08239.t1 AT5G48150 29.639 388 240 10 120 495 123 489 1.13e-42 159
MS.gene08239.t1 AT2G01570 30.882 408 236 14 107 501 212 586 5.84e-41 155
MS.gene08239.t1 AT4G17230 31.608 367 213 10 144 495 181 524 1.61e-40 153
MS.gene08239.t1 AT4G17230 31.608 367 213 10 144 495 181 524 1.61e-40 153
MS.gene08239.t1 AT1G21450 31.043 393 232 14 118 495 224 592 2.21e-40 154
MS.gene08239.t1 AT1G14920 29.756 410 236 16 107 500 160 533 5.15e-39 149
MS.gene08239.t1 AT2G04890 30.905 398 231 14 111 495 46 412 6.61e-39 146
MS.gene08239.t1 AT1G50600 30.789 380 229 12 130 495 166 525 2.03e-37 144
MS.gene08239.t1 AT1G50600 30.789 380 229 12 130 495 237 596 3.73e-37 145
MS.gene08239.t1 AT1G50600 30.789 380 229 12 130 495 241 600 3.75e-37 145
MS.gene08239.t1 AT1G07530 27.938 451 281 13 65 500 348 769 3.83e-37 146
MS.gene08239.t1 AT1G63100 29.078 423 262 13 93 496 226 629 3.73e-36 142
MS.gene08239.t1 AT1G63100 29.078 423 262 13 93 496 251 654 3.88e-36 142
MS.gene08239.t1 AT2G29065 26.598 391 260 10 119 496 252 628 6.95e-32 130
MS.gene08239.t1 AT2G29060 25.745 470 290 17 51 496 221 655 1.37e-31 129
MS.gene08239.t1 AT2G29060 25.745 470 290 17 51 496 258 692 1.37e-31 129
MS.gene08239.t1 AT1G07520 27.222 360 234 9 154 498 350 696 7.69e-31 127
MS.gene08239.t1 AT1G07520 27.222 360 234 9 154 498 185 531 7.71e-31 125
MS.gene08239.t1 AT1G07520 27.222 360 234 9 154 498 185 531 7.71e-31 125
MS.gene08239.t1 AT3G46600 25.778 450 284 12 66 495 159 578 7.78e-31 126
MS.gene08239.t1 AT1G07520 27.222 360 234 9 154 498 349 695 7.95e-31 127
MS.gene08239.t1 AT3G46600 27.889 398 237 12 118 495 3 370 8.56e-31 123
MS.gene08239.t1 AT3G46600 25.778 450 284 12 66 495 127 546 1.47e-30 125
MS.gene08239.t1 AT4G37650 28.070 399 249 14 119 495 146 528 1.04e-29 122
MS.gene08239.t1 AT5G59450 25.000 452 293 12 68 495 168 597 2.20e-29 122
MS.gene08239.t1 AT3G60630 28.218 404 256 12 110 497 238 623 1.88e-28 119
MS.gene08239.t1 AT2G37650 28.250 400 252 13 114 501 330 706 6.13e-27 115
MS.gene08239.t1 AT2G37650 28.250 400 252 13 114 501 342 718 7.94e-27 115
MS.gene08239.t1 AT2G45160 27.251 411 246 14 108 497 262 640 5.98e-26 112
MS.gene08239.t1 AT4G36710 27.913 369 233 12 136 495 140 484 8.36e-26 110
MS.gene08239.t1 AT4G00150 27.020 396 228 13 120 500 209 558 9.54e-21 95.9
MS.gene08239.t1 AT1G50420 32.203 236 135 9 105 335 43 258 2.71e-20 94.0
MS.gene08239.t1 AT1G50420 34.641 153 94 3 348 497 331 480 7.17e-18 86.7
MS.gene08239.t1 AT3G49950 25.469 373 225 13 153 496 60 408 3.52e-19 90.1
MS.gene08239.t1 AT5G52510 28.869 336 171 12 202 495 330 639 9.06e-19 90.1

Find 196 sgRNAs with CRISPR-Local

Find 211 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTAGGTGAGGTAGGGTTTA+GGG 0.226810 3.2:-65471157 MS.gene08239:CDS
CCTACTTCCGACGATGATTA+TGG 0.250428 3.2:-65472401 MS.gene08239:CDS
GTCAACTGGGACACCTTTAC+AGG 0.297676 3.2:-65472350 MS.gene08239:CDS
GCAGAACCGGGCCCGGGCTT+TGG 0.319278 3.2:-65471274 MS.gene08239:CDS
CATTTCACCGAAGCCCTTCA+TGG 0.325802 3.2:-65471984 MS.gene08239:CDS
TGTCAAGCAAAGCTGTTGTT+AGG 0.326046 3.2:-65471108 MS.gene08239:CDS
TGGGTTTGTGGAAAGGTTCA+TGG 0.326737 3.2:-65471352 MS.gene08239:CDS
GGTTAGGTGAGGTAGGGTTT+AGG 0.331084 3.2:-65471158 MS.gene08239:CDS
ACTGGTCTCCCATCGTCAAC+TGG 0.335690 3.2:-65472364 MS.gene08239:CDS
AGCTTCGATGATGGCTTGAT+TGG 0.338111 3.2:+65471806 None:intergenic
GGTGGTGGTGGTTGTGGTGA+TGG 0.340117 3.2:+65472250 None:intergenic
GGAGAGTGGTTAGGTGAGGT+AGG 0.352680 3.2:-65471165 MS.gene08239:CDS
TCCACCAACGTCACTAGCTT+TGG 0.358956 3.2:+65471399 None:intergenic
GTGGTGGTTGTGGTGATGGA+TGG 0.363760 3.2:+65472254 None:intergenic
ATCAATGACCTCCACTTCTC+TGG 0.368955 3.2:-65472446 MS.gene08239:CDS
ACCTCCTTCGCCGCTTCCCT+TGG 0.370284 3.2:-65471600 MS.gene08239:CDS
CCTAGGATTGCAGGCTCGTT+GGG 0.371680 3.2:-65471231 MS.gene08239:CDS
GGTTGTGGTGGTGGTGGTTG+TGG 0.377950 3.2:+65472244 None:intergenic
TTCCGATGCAGAACCGGGCC+CGG 0.381021 3.2:-65471281 MS.gene08239:CDS
TTCGGTAGTTGGTGGGTTTG+TGG 0.382194 3.2:-65471364 MS.gene08239:CDS
GCTTGTACGTGGAGAGGCTT+TGG 0.384610 3.2:-65471511 MS.gene08239:CDS
CTTCCGCGCCAGCCATCAAT+AGG 0.391873 3.2:+65472117 None:intergenic
ATGAGGTGGTGAAAGTCATC+TGG 0.398824 3.2:+65472323 None:intergenic
GCCTAGGATTGCAGGCTCGT+TGG 0.400453 3.2:-65471232 MS.gene08239:CDS
GAAGAAGTTGGTTCGGTAGT+TGG 0.401602 3.2:-65471375 MS.gene08239:CDS
GGTAGGGTTTAGGGGAGTTC+CGG 0.404698 3.2:-65471148 MS.gene08239:CDS
TTCTTCCTCATCGGTGGTTG+TGG 0.404871 3.2:+65472229 None:intergenic
TCCGATGCAGAACCGGGCCC+GGG 0.407928 3.2:-65471280 MS.gene08239:CDS
CGATGAGGAAGAAGAAGAAA+TGG 0.410332 3.2:-65472219 MS.gene08239:CDS
AAGCAGCAAGTGTGTCGTTC+TGG 0.410739 3.2:+65471880 None:intergenic
CATTTCTTCTTCTTCCTCAT+CGG 0.412349 3.2:+65472220 None:intergenic
GAACCGGGCCCGGGCTTTGG+TGG 0.417856 3.2:-65471271 MS.gene08239:CDS
GAAGGACGGAAAGTTTCATC+TGG 0.418561 3.2:+65471543 None:intergenic
GTTTGCTTTCTGCTTCCCTT+TGG 0.430877 3.2:-65471008 MS.gene08239:CDS
CTCATCGGTGGTTGTGGTGG+TGG 0.431811 3.2:+65472235 None:intergenic
GCATTATCACGGACAGGAAC+CGG 0.434140 3.2:-65471666 MS.gene08239:CDS
CCCAACGAGCCTGCAATCCT+AGG 0.435603 3.2:+65471231 None:intergenic
CATAATCATCGTCGGAAGTA+GGG 0.435805 3.2:+65472402 None:intergenic
TCGCCTGCCGGTTTCTTGTA+CGG 0.437235 3.2:+65471626 None:intergenic
CACCACCCGTTCTATATATC+CGG 0.439796 3.2:+65471208 None:intergenic
GTAATAAGTTGGTGTTGGAT+TGG 0.441173 3.2:-65471041 MS.gene08239:CDS
GCCCTTCATGGACTACTAGA+AGG 0.443992 3.2:-65471972 MS.gene08239:CDS
GAAGTTGGTGATGATTCAAA+AGG 0.445291 3.2:-65472155 MS.gene08239:CDS
AAGAATGAAGCAACTGATTC+CGG 0.447596 3.2:+65471444 None:intergenic
TGACATGGACGCGGCGCTCG+TGG 0.452405 3.2:+65471778 None:intergenic
AGAGAAGGTCGTGGGGAGAG+TGG 0.453369 3.2:-65471179 MS.gene08239:CDS
GAGAGTGGTTAGGTGAGGTA+GGG 0.454793 3.2:-65471164 MS.gene08239:CDS
TGAGGTAATGTTTGTTGTTG+TGG 0.455641 3.2:+65471925 None:intergenic
AAAACTAGTGCACCTATTGA+TGG 0.457233 3.2:-65472129 MS.gene08239:CDS
GATGGGAGACCAGTGGTTCC+AGG 0.457583 3.2:+65472373 None:intergenic
ATGTCACCTTACGTCAAATT+CGG 0.460085 3.2:-65471840 MS.gene08239:CDS
AGAGAAGTGGAGGTCATTGA+TGG 0.464377 3.2:+65472448 None:intergenic
AAACATTACCTCACCACAAA+TGG 0.466163 3.2:-65471915 MS.gene08239:CDS
TGAAGAGGAGAGAAGGTCGT+GGG 0.469399 3.2:-65471187 MS.gene08239:CDS
CCATAATCATCGTCGGAAGT+AGG 0.475182 3.2:+65472401 None:intergenic
AAGATGAGGACCGTTGTTGT+TGG 0.475317 3.2:+65471698 None:intergenic
AGGTCGTGGGGAGAGTGGTT+AGG 0.475697 3.2:-65471174 MS.gene08239:CDS
CATGGACTACTAGAAGGTGC+TGG 0.477897 3.2:-65471966 MS.gene08239:CDS
TCTCCACCAAAGCCCGGGCC+CGG 0.479183 3.2:+65471268 None:intergenic
GTGGGCCACAGCTTCGATGA+TGG 0.479536 3.2:+65471797 None:intergenic
ACAACAACGGTCCTCATCTT+CGG 0.479785 3.2:-65471695 MS.gene08239:CDS
CGGAAGTAGGGGTGTTGGTG+AGG 0.483268 3.2:+65472414 None:intergenic
CGGGTATAGGGTTGAAGAGG+TGG 0.483638 3.2:-65471073 MS.gene08239:CDS
GTGTGTCGTTCTGGTTGTCA+TGG 0.484328 3.2:+65471889 None:intergenic
GGCCCGGGCTTTGGTGGAGA+GGG 0.485841 3.2:-65471265 MS.gene08239:CDS
ATACCCTCTCCACCAAAGCC+CGG 0.489626 3.2:+65471262 None:intergenic
GTCAAGCAAAGCTGTTGTTA+GGG 0.489648 3.2:-65471107 MS.gene08239:CDS
GCTGGCGCGGAAGCCTTAAC+CGG 0.490918 3.2:-65472107 MS.gene08239:CDS
ATCGCCACCGTACAAGAAAC+CGG 0.491760 3.2:-65471633 MS.gene08239:CDS
TAGGGTTGAAGAGGTGGGTG+TGG 0.493351 3.2:-65471067 MS.gene08239:CDS
ATTCCGGTGCACGGTAACTA+AGG 0.494877 3.2:+65471460 None:intergenic
TAGTAGTCCATGAAGGGCTT+CGG 0.495192 3.2:+65471977 None:intergenic
ATGGTTGCACCTGGAACCAC+TGG 0.499209 3.2:-65472382 MS.gene08239:CDS
ATCGGTGGTTGTGGTGGTGG+TGG 0.499565 3.2:+65472238 None:intergenic
TAGTTGGTGGGTTTGTGGAA+AGG 0.499576 3.2:-65471359 MS.gene08239:CDS
TTCTGTCGTTGTTGTTGTTG+TGG 0.501931 3.2:+65472187 None:intergenic
GTCATCTGGGGCACCTGTAA+AGG 0.502615 3.2:+65472337 None:intergenic
TTGTCCAAGGGAAGCGGCGA+AGG 0.503279 3.2:+65471596 None:intergenic
GTGACATGTCTTGAAGTAGT+TGG 0.504360 3.2:+65471856 None:intergenic
GGAAGTAGGGGTGTTGGTGA+GGG 0.508441 3.2:+65472415 None:intergenic
CATCATGGAAGGGGTCCAAT+GGG 0.510759 3.2:-65471745 MS.gene08239:CDS
CACCTTCTAGTAGTCCATGA+AGG 0.513198 3.2:+65471970 None:intergenic
TTCTTCTTCTTCCTCATCGG+TGG 0.515776 3.2:+65472223 None:intergenic
TGTGTCGTTCTGGTTGTCAT+GGG 0.515837 3.2:+65471890 None:intergenic
AGAAGAAGAACAAGTCCAAA+GGG 0.515921 3.2:+65470993 None:intergenic
TGTTGTTGGAAGCTAGTGAT+TGG 0.517924 3.2:+65471712 None:intergenic
GGATTGCAGGCTCGTTGGGC+CGG 0.518025 3.2:-65471227 MS.gene08239:CDS
GACATGGACGCGGCGCTCGT+GGG 0.519310 3.2:+65471779 None:intergenic
GGGCCCGGGCTTTGGTGGAG+AGG 0.520373 3.2:-65471266 MS.gene08239:CDS
TGGTGTTGGATTGGAAATCA+AGG 0.521049 3.2:-65471032 MS.gene08239:CDS
ATGGACTACTAGAAGGTGCT+GGG 0.522101 3.2:-65471965 MS.gene08239:CDS
GTGAAGAGGAGAGAAGGTCG+TGG 0.522620 3.2:-65471188 MS.gene08239:CDS
GTACGGTGGCGATTGACCTC+CGG 0.522939 3.2:+65471643 None:intergenic
TACCCTCTCCACCAAAGCCC+GGG 0.525259 3.2:+65471263 None:intergenic
ATATAGAACGGGTGGTGAAG+AGG 0.526637 3.2:-65471202 MS.gene08239:CDS
CTGCAGTGGTGGAAAGAAAA+CGG 0.528193 3.2:+65471573 None:intergenic
ACATCATGGAAGGGGTCCAA+TGG 0.528843 3.2:-65471746 MS.gene08239:CDS
TTAGGTGAGGTAGGGTTTAG+GGG 0.528886 3.2:-65471156 MS.gene08239:CDS
CTAGTGCACCTATTGATGGC+TGG 0.529907 3.2:-65472125 MS.gene08239:CDS
GGGAGCTGTGTCCAGAGAAG+TGG 0.530921 3.2:+65472435 None:intergenic
GGGTAGTAATAAGTTGGTGT+TGG 0.531656 3.2:-65471046 MS.gene08239:CDS
CAGTAATACCATGGACATGA+TGG 0.531857 3.2:-65472480 None:intergenic
AAGTTGGTTCGGTAGTTGGT+GGG 0.534092 3.2:-65471371 MS.gene08239:CDS
GGTGGAAGAAGAAGTTGGTT+CGG 0.535127 3.2:-65471382 MS.gene08239:CDS
GAAGTTGGTTCGGTAGTTGG+TGG 0.538694 3.2:-65471372 MS.gene08239:CDS
CTGGTCTCCCATCGTCAACT+GGG 0.539421 3.2:-65472363 MS.gene08239:CDS
GTTGTCATGGGGACCATTTG+TGG 0.540348 3.2:+65471902 None:intergenic
CCTGCCGGTTTCTTGTACGG+TGG 0.541192 3.2:+65471629 None:intergenic
ACATGCTAACGGCTCTAACA+TGG 0.542095 3.2:-65472021 MS.gene08239:CDS
CAATGATTTGCAAAGCTCAC+CGG 0.544682 3.2:+65471129 None:intergenic
GCCCGGGCCCGGTTCTGCAT+CGG 0.547611 3.2:+65471279 None:intergenic
GGGTGTGGGTAGTAATAAGT+TGG 0.548725 3.2:-65471052 MS.gene08239:CDS
GTGAGACTAATTCCTTGAGC+CGG 0.552046 3.2:+65472045 None:intergenic
CGGGTGGTGAAGAGGAGAGA+AGG 0.553224 3.2:-65471194 MS.gene08239:CDS
TGTCATCGACTACGACATCA+TGG 0.554847 3.2:-65471760 MS.gene08239:CDS
TGAGGTGGTGAAAGTCATCT+GGG 0.555233 3.2:+65472324 None:intergenic
TAGCTCGAGTGATATTGATC+CGG 0.555972 3.2:-65472064 MS.gene08239:CDS
AGGGTTGAAGAGGTGGGTGT+GGG 0.556341 3.2:-65471066 MS.gene08239:CDS
TTCCTCATCGGTGGTTGTGG+TGG 0.557874 3.2:+65472232 None:intergenic
ATCGTCGGAAGTAGGGGTGT+TGG 0.558472 3.2:+65472409 None:intergenic
ATCCATGTCCATCATGTCCA+TGG 0.558666 3.2:+65472472 None:intergenic
GGAAAGAAAACGGTTGTCCA+AGG 0.560068 3.2:+65471583 None:intergenic
TCAGTTGCTTCATTCTTGAA+CGG 0.560073 3.2:-65471438 MS.gene08239:CDS
GAAGAGGAGAGAAGGTCGTG+GGG 0.560976 3.2:-65471186 MS.gene08239:CDS
TTCGGATAACCGCATTATCA+CGG 0.561276 3.2:-65471677 MS.gene08239:CDS
GGTAATGTTTGTTGTTGTGG+TGG 0.561567 3.2:+65471928 None:intergenic
AGATGACTTTCACCACCTCA+TGG 0.561702 3.2:-65472321 MS.gene08239:CDS
CTGGGTCTAACCTGCAGTGG+TGG 0.565647 3.2:+65471562 None:intergenic
TTGGGCCGGATATATAGAAC+GGG 0.569905 3.2:-65471213 MS.gene08239:CDS
ATTGGATTAATGAAGCCCAT+TGG 0.571351 3.2:+65471730 None:intergenic
AAACGGTTGTCCAAGGGAAG+CGG 0.571859 3.2:+65471590 None:intergenic
TCACCTTAGTTACCGTGCAC+CGG 0.573305 3.2:-65471463 MS.gene08239:CDS
GGGTATAGGGTTGAAGAGGT+GGG 0.575639 3.2:-65471072 MS.gene08239:CDS
ATCGACTACGACATCATGGA+AGG 0.578928 3.2:-65471756 MS.gene08239:CDS
CTCACTTCATCACTATTCAG+CGG 0.580797 3.2:-65471328 MS.gene08239:CDS
CCACCGTACAAGAAACCGGC+AGG 0.581224 3.2:-65471629 MS.gene08239:CDS
CGACGGGTATAGGGTTGAAG+AGG 0.581699 3.2:-65471076 MS.gene08239:CDS
TCCAAGGGAAGCGGCGAAGG+AGG 0.582127 3.2:+65471599 None:intergenic
ACGATGATTATGGTTGCACC+TGG 0.582304 3.2:-65472391 MS.gene08239:CDS
AGTGATATTGATCCGGCTCA+AGG 0.583439 3.2:-65472057 MS.gene08239:CDS
AGCTGTGTCCAGAGAAGTGG+AGG 0.585513 3.2:+65472438 None:intergenic
CATCTGGGTCTAACCTGCAG+TGG 0.586227 3.2:+65471559 None:intergenic
AAGGTGTCCCAGTTGACGAT+GGG 0.586507 3.2:+65472356 None:intergenic
TCCAAAGCTAGTGACGTTGG+TGG 0.588911 3.2:-65471400 MS.gene08239:CDS
AAGAAGAAGAACAAGTCCAA+AGG 0.591374 3.2:+65470992 None:intergenic
AAGTTGGTGATGATTCAAAA+GGG 0.593257 3.2:-65472154 MS.gene08239:CDS
GAAGGAGGTTAATCGCCTGC+CGG 0.597149 3.2:+65471614 None:intergenic
CGACTACGACATCATGGAAG+GGG 0.598773 3.2:-65471754 MS.gene08239:CDS
ACCTTCTAGTAGTCCATGAA+GGG 0.600557 3.2:+65471971 None:intergenic
GTGGGGAGAGTGGTTAGGTG+AGG 0.605688 3.2:-65471169 MS.gene08239:CDS
GCACCTATTGATGGCTGGCG+CGG 0.609141 3.2:-65472120 MS.gene08239:CDS
ATTAAAGCTTGTACGTGGAG+AGG 0.611742 3.2:-65471517 MS.gene08239:CDS
ATAATCATCGTCGGAAGTAG+GGG 0.612654 3.2:+65472403 None:intergenic
TCAAGCCATCATCGAAGCTG+TGG 0.613643 3.2:-65471802 MS.gene08239:CDS
AAGCAACTGATTCCGGTGCA+CGG 0.618564 3.2:+65471451 None:intergenic
CGTACAAGCTTTAATGCAGA+AGG 0.623940 3.2:+65471525 None:intergenic
TTAGTCTCACAACATGCTAA+CGG 0.625561 3.2:-65472032 MS.gene08239:CDS
CACGGACAGGAACCGGCCGG+AGG 0.629234 3.2:-65471659 MS.gene08239:CDS
AATGCGGTTATCCGAAGATG+AGG 0.632509 3.2:+65471684 None:intergenic
AAAGGTGTCCCAGTTGACGA+TGG 0.634828 3.2:+65472355 None:intergenic
TGGATGGACTAAGCTGGTCG+AGG 0.635528 3.2:+65472270 None:intergenic
GTTGGGCCGGATATATAGAA+CGG 0.635781 3.2:-65471214 MS.gene08239:CDS
AAGTGGCCGAATTTGACGTA+AGG 0.638521 3.2:+65471834 None:intergenic
AAGGACGGAAAGTTTCATCT+GGG 0.639197 3.2:+65471544 None:intergenic
ACGTTGGTGGAAGAAGAAGT+TGG 0.642595 3.2:-65471387 MS.gene08239:CDS
GGTTCCTGTCCGTGATAATG+CGG 0.642931 3.2:+65471668 None:intergenic
CGATGATTGTGTCCATGAGG+TGG 0.643118 3.2:+65472309 None:intergenic
TCGACTACGACATCATGGAA+GGG 0.644449 3.2:-65471755 MS.gene08239:CDS
CACCACCACAACCACCGATG+AGG 0.645241 3.2:-65472234 MS.gene08239:CDS
ATAACCGCATTATCACGGAC+AGG 0.646058 3.2:-65471672 MS.gene08239:CDS
TATCACGGACAGGAACCGGC+CGG 0.647005 3.2:-65471662 MS.gene08239:CDS
TGATGTCGTAGTCGATGACA+TGG 0.647412 3.2:+65471763 None:intergenic
CGTAGTCGATGACATGGACG+CGG 0.647870 3.2:+65471769 None:intergenic
ATATCACTCGAGCTAAGTCA+CGG 0.648503 3.2:+65472072 None:intergenic
TGGCTTGATTGGCTGTGAAG+TGG 0.648617 3.2:+65471817 None:intergenic
ACTAAGCTGGTCGAGGACAG+TGG 0.650840 3.2:+65472277 None:intergenic
GAAAGAAAACGGTTGTCCAA+GGG 0.651213 3.2:+65471584 None:intergenic
GAATCCAAAGCTAGTGACGT+TGG 0.651578 3.2:-65471403 MS.gene08239:CDS
TGGACTACTAGAAGGTGCTG+GGG 0.652604 3.2:-65471964 MS.gene08239:CDS
CATGGGGACCATTTGTGGTG+AGG 0.653840 3.2:+65471907 None:intergenic
AAGGGCTTCGGTGAAATGAG+CGG 0.654823 3.2:+65471989 None:intergenic
TCTGCATTAAAGCTTGTACG+TGG 0.657776 3.2:-65471522 MS.gene08239:CDS
TACCATGGACATGATGGACA+TGG 0.658713 3.2:-65472474 None:intergenic
TGGTGATGGATGGACTAAGC+TGG 0.659130 3.2:+65472264 None:intergenic
GGCCGGATATATAGAACGGG+TGG 0.661675 3.2:-65471210 MS.gene08239:CDS
GGTGCAACCATAATCATCGT+CGG 0.661939 3.2:+65472394 None:intergenic
CAAGCTTTAATGCAGAAGGA+CGG 0.671677 3.2:+65471529 None:intergenic
GGACTACTAGAAGGTGCTGG+GGG 0.674008 3.2:-65471963 MS.gene08239:CDS
GTGTCGTTCTGGTTGTCATG+GGG 0.680681 3.2:+65471891 None:intergenic
GGTGGCGATTGACCTCCGGC+CGG 0.683391 3.2:+65471647 None:intergenic
AGTTGACGATGGGAGACCAG+TGG 0.695859 3.2:+65472366 None:intergenic
GGTGCACGGTAACTAAGGTG+AGG 0.709219 3.2:+65471465 None:intergenic
TCACTAGCTTCCAACAACAA+CGG 0.720928 3.2:-65471708 MS.gene08239:CDS
GAGGTGGTGAAAGTCATCTG+GGG 0.725269 3.2:+65472325 None:intergenic
CCTCGATGATTGTGTCCATG+AGG 0.737785 3.2:+65472306 None:intergenic
CCTCATGGACACAATCATCG+AGG 0.768679 3.2:-65472306 MS.gene08239:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ACAGAAAAAACACAAGAAGT+TGG - chr3.2:65471275-65471294 MS.gene08239:CDS 30.0%
!! AAGTTGGTGATGATTCAAAA+GGG - chr3.2:65471291-65471310 MS.gene08239:CDS 30.0%
AAAACTAGTGCACCTATTGA+TGG - chr3.2:65471316-65471335 MS.gene08239:CDS 35.0%
AAACATTACCTCACCACAAA+TGG - chr3.2:65471530-65471549 MS.gene08239:CDS 35.0%
AAGAAGAAGAACAAGTCCAA+AGG + chr3.2:65472456-65472475 None:intergenic 35.0%
AGAAGAAGAACAAGTCCAAA+GGG + chr3.2:65472455-65472474 None:intergenic 35.0%
ATGTCACCTTACGTCAAATT+CGG - chr3.2:65471605-65471624 MS.gene08239:CDS 35.0%
ATTGGATTAATGAAGCCCAT+TGG + chr3.2:65471718-65471737 None:intergenic 35.0%
CATTTCTTCTTCTTCCTCAT+CGG + chr3.2:65471228-65471247 None:intergenic 35.0%
TCAGTTGCTTCATTCTTGAA+CGG - chr3.2:65472007-65472026 MS.gene08239:CDS 35.0%
TTAGTCTCACAACATGCTAA+CGG - chr3.2:65471413-65471432 MS.gene08239:CDS 35.0%
! AAGAATGAAGCAACTGATTC+CGG + chr3.2:65472004-65472023 None:intergenic 35.0%
! TTGTTAGGGCTTTTTAACGA+CGG - chr3.2:65472352-65472371 MS.gene08239:CDS 35.0%
!! GAAGTTGGTGATGATTCAAA+AGG - chr3.2:65471290-65471309 MS.gene08239:CDS 35.0%
!! GTAATAAGTTGGTGTTGGAT+TGG - chr3.2:65472404-65472423 MS.gene08239:CDS 35.0%
!! TGAGGTAATGTTTGTTGTTG+TGG + chr3.2:65471523-65471542 None:intergenic 35.0%
!! TTCAGCGGTTTTTGATTCAT+TGG - chr3.2:65472132-65472151 MS.gene08239:CDS 35.0%
AAGGACGGAAAGTTTCATCT+GGG + chr3.2:65471904-65471923 None:intergenic 40.0%
ACCTTCTAGTAGTCCATGAA+GGG + chr3.2:65471477-65471496 None:intergenic 40.0%
ATAATCATCGTCGGAAGTAG+GGG + chr3.2:65471045-65471064 None:intergenic 40.0%
ATATCACTCGAGCTAAGTCA+CGG + chr3.2:65471376-65471395 None:intergenic 40.0%
ATTAAAGCTTGTACGTGGAG+AGG - chr3.2:65471928-65471947 MS.gene08239:CDS 40.0%
CATAATCATCGTCGGAAGTA+GGG + chr3.2:65471046-65471065 None:intergenic 40.0%
CGATGAGGAAGAAGAAGAAA+TGG - chr3.2:65471226-65471245 MS.gene08239:CDS 40.0%
CTCACTTCATCACTATTCAG+CGG - chr3.2:65472117-65472136 MS.gene08239:CDS 40.0%
GAAAGAAAACGGTTGTCCAA+GGG + chr3.2:65471864-65471883 None:intergenic 40.0%
GTCAAGCAAAGCTGTTGTTA+GGG - chr3.2:65472338-65472357 MS.gene08239:CDS 40.0%
GTGACATGTCTTGAAGTAGT+TGG + chr3.2:65471592-65471611 None:intergenic 40.0%
TAGCTCGAGTGATATTGATC+CGG - chr3.2:65471381-65471400 MS.gene08239:CDS 40.0%
TCTGCATTAAAGCTTGTACG+TGG - chr3.2:65471923-65471942 MS.gene08239:CDS 40.0%
TGTCAAGCAAAGCTGTTGTT+AGG - chr3.2:65472337-65472356 MS.gene08239:CDS 40.0%
TGTTGTTGGAAGCTAGTGAT+TGG + chr3.2:65471736-65471755 None:intergenic 40.0%
TTCGGATAACCGCATTATCA+CGG - chr3.2:65471768-65471787 MS.gene08239:CDS 40.0%
! CAAGCTTTAATGCAGAAGGA+CGG + chr3.2:65471919-65471938 None:intergenic 40.0%
! CAATGATTTGCAAAGCTCAC+CGG + chr3.2:65472319-65472338 None:intergenic 40.0%
! CGTACAAGCTTTAATGCAGA+AGG + chr3.2:65471923-65471942 None:intergenic 40.0%
! GCTTTTTAACGACGGGTATA+GGG - chr3.2:65472360-65472379 MS.gene08239:CDS 40.0%
! TCACTAGCTTCCAACAACAA+CGG - chr3.2:65471737-65471756 MS.gene08239:CDS 40.0%
! TGGTGTTGGATTGGAAATCA+AGG - chr3.2:65472413-65472432 MS.gene08239:CDS 40.0%
! TGTTAGGGCTTTTTAACGAC+GGG - chr3.2:65472353-65472372 MS.gene08239:CDS 40.0%
! TTCTGTCGTTGTTGTTGTTG+TGG + chr3.2:65471261-65471280 None:intergenic 40.0%
!! AGCGGTTTTTGATTCATTGG+AGG - chr3.2:65472135-65472154 MS.gene08239:CDS 40.0%
!! GGGTAGTAATAAGTTGGTGT+TGG - chr3.2:65472399-65472418 MS.gene08239:CDS 40.0%
!! GGTAATGTTTGTTGTTGTGG+TGG + chr3.2:65471520-65471539 None:intergenic 40.0%
!! GTTTTTGATTCATTGGAGGC+TGG - chr3.2:65472139-65472158 MS.gene08239:CDS 40.0%
!!! GTTTTTGGTTGAACCGGTTA+AGG + chr3.2:65471354-65471373 None:intergenic 40.0%
!!! TGGTGGAGAGGGTATTTTTT+GGG - chr3.2:65472191-65472210 MS.gene08239:CDS 40.0%
!!! TTGGTGGAGAGGGTATTTTT+TGG - chr3.2:65472190-65472209 MS.gene08239:CDS 40.0%
AAGATGAGGACCGTTGTTGT+TGG + chr3.2:65471750-65471769 None:intergenic 45.0%
AAGTGGCCGAATTTGACGTA+AGG + chr3.2:65471614-65471633 None:intergenic 45.0%
AATGCGGTTATCCGAAGATG+AGG + chr3.2:65471764-65471783 None:intergenic 45.0%
ACAACAACGGTCCTCATCTT+CGG - chr3.2:65471750-65471769 MS.gene08239:CDS 45.0%
ACGATGATTATGGTTGCACC+TGG - chr3.2:65471054-65471073 MS.gene08239:CDS 45.0%
AGAGAAGTGGAGGTCATTGA+TGG + chr3.2:65471000-65471019 None:intergenic 45.0%
AGATGACTTTCACCACCTCA+TGG - chr3.2:65471124-65471143 MS.gene08239:CDS 45.0%
AGTGATATTGATCCGGCTCA+AGG - chr3.2:65471388-65471407 MS.gene08239:CDS 45.0%
ATAACCGCATTATCACGGAC+AGG - chr3.2:65471773-65471792 MS.gene08239:CDS 45.0%
ATATAGAACGGGTGGTGAAG+AGG - chr3.2:65472243-65472262 MS.gene08239:CDS 45.0%
ATCAATGACCTCCACTTCTC+TGG - chr3.2:65470999-65471018 MS.gene08239:CDS 45.0%
ATCGACTACGACATCATGGA+AGG - chr3.2:65471689-65471708 MS.gene08239:CDS 45.0%
ATGAGGTGGTGAAAGTCATC+TGG + chr3.2:65471125-65471144 None:intergenic 45.0%
CACCACCCGTTCTATATATC+CGG + chr3.2:65472240-65472259 None:intergenic 45.0%
CACCTTCTAGTAGTCCATGA+AGG + chr3.2:65471478-65471497 None:intergenic 45.0%
CCATAATCATCGTCGGAAGT+AGG + chr3.2:65471047-65471066 None:intergenic 45.0%
CCTACTTCCGACGATGATTA+TGG - chr3.2:65471044-65471063 MS.gene08239:CDS 45.0%
GAAGGACGGAAAGTTTCATC+TGG + chr3.2:65471905-65471924 None:intergenic 45.0%
GAATCCAAAGCTAGTGACGT+TGG - chr3.2:65472042-65472061 MS.gene08239:CDS 45.0%
GGAAAGAAAACGGTTGTCCA+AGG + chr3.2:65471865-65471884 None:intergenic 45.0%
GGGTGTGGGTAGTAATAAGT+TGG - chr3.2:65472393-65472412 MS.gene08239:CDS 45.0%
GGTGCAACCATAATCATCGT+CGG + chr3.2:65471054-65471073 None:intergenic 45.0%
GTGAGACTAATTCCTTGAGC+CGG + chr3.2:65471403-65471422 None:intergenic 45.0%
GTTAGGTGAGGTAGGGTTTA+GGG - chr3.2:65472288-65472307 MS.gene08239:CDS 45.0%
GTTGGGCCGGATATATAGAA+CGG - chr3.2:65472231-65472250 MS.gene08239:CDS 45.0%
GTTTGCTTTCTGCTTCCCTT+TGG - chr3.2:65472437-65472456 MS.gene08239:CDS 45.0%
TAGTAGTCCATGAAGGGCTT+CGG + chr3.2:65471471-65471490 None:intergenic 45.0%
TCGACTACGACATCATGGAA+GGG - chr3.2:65471690-65471709 MS.gene08239:CDS 45.0%
TGAGGTGGTGAAAGTCATCT+GGG + chr3.2:65471124-65471143 None:intergenic 45.0%
TGATGTCGTAGTCGATGACA+TGG + chr3.2:65471685-65471704 None:intergenic 45.0%
TGGGTTTGTGGAAAGGTTCA+TGG - chr3.2:65472093-65472112 MS.gene08239:CDS 45.0%
TTAGGTGAGGTAGGGTTTAG+GGG - chr3.2:65472289-65472308 MS.gene08239:CDS 45.0%
TTCTTCTTCTTCCTCATCGG+TGG + chr3.2:65471225-65471244 None:intergenic 45.0%
TTGGGCCGGATATATAGAAC+GGG - chr3.2:65472232-65472251 MS.gene08239:CDS 45.0%
! ACATGCTAACGGCTCTAACA+TGG - chr3.2:65471424-65471443 MS.gene08239:CDS 45.0%
! ACGTTGGTGGAAGAAGAAGT+TGG - chr3.2:65472058-65472077 MS.gene08239:CDS 45.0%
! AGCTTCGATGATGGCTTGAT+TGG + chr3.2:65471642-65471661 None:intergenic 45.0%
! CTGCAGTGGTGGAAAGAAAA+CGG + chr3.2:65471875-65471894 None:intergenic 45.0%
! GGCTTTTTAACGACGGGTAT+AGG - chr3.2:65472359-65472378 MS.gene08239:CDS 45.0%
! GGTGGAAGAAGAAGTTGGTT+CGG - chr3.2:65472063-65472082 MS.gene08239:CDS 45.0%
! TCGAGCTAAGTCACGGTTTT+TGG + chr3.2:65471369-65471388 None:intergenic 45.0%
! TGTCATCGACTACGACATCA+TGG - chr3.2:65471685-65471704 MS.gene08239:CDS 45.0%
! TGTGTCGTTCTGGTTGTCAT+GGG + chr3.2:65471558-65471577 None:intergenic 45.0%
!! AAGTTGGTTCGGTAGTTGGT+GGG - chr3.2:65472074-65472093 MS.gene08239:CDS 45.0%
!! ATGGACTACTAGAAGGTGCT+GGG - chr3.2:65471480-65471499 MS.gene08239:CDS 45.0%
!! GAAGAAGTTGGTTCGGTAGT+TGG - chr3.2:65472070-65472089 MS.gene08239:CDS 45.0%
!! TAGTTGGTGGGTTTGTGGAA+AGG - chr3.2:65472086-65472105 MS.gene08239:CDS 45.0%
!!! AGAGGGTATTTTTTGGGCCT+AGG - chr3.2:65472197-65472216 MS.gene08239:CDS 45.0%
!!! GTCACGGTTTTTGGTTGAAC+CGG + chr3.2:65471360-65471379 None:intergenic 45.0%
!!! TTTTTTGGGCCTAGGATTGC+AGG - chr3.2:65472205-65472224 MS.gene08239:CDS 45.0%
AAACGGTTGTCCAAGGGAAG+CGG + chr3.2:65471858-65471877 None:intergenic 50.0%
AAGGGCTTCGGTGAAATGAG+CGG + chr3.2:65471459-65471478 None:intergenic 50.0%
ACATCATGGAAGGGGTCCAA+TGG - chr3.2:65471699-65471718 MS.gene08239:CDS 50.0%
ATCGCCACCGTACAAGAAAC+CGG - chr3.2:65471812-65471831 MS.gene08239:CDS 50.0%
ATTCCGGTGCACGGTAACTA+AGG + chr3.2:65471988-65472007 None:intergenic 50.0%
CATCATGGAAGGGGTCCAAT+GGG - chr3.2:65471700-65471719 MS.gene08239:CDS 50.0%
CATTTCACCGAAGCCCTTCA+TGG - chr3.2:65471461-65471480 MS.gene08239:CDS 50.0%
CCTCATGGACACAATCATCG+AGG - chr3.2:65471139-65471158 MS.gene08239:CDS 50.0%
CCTCGATGATTGTGTCCATG+AGG + chr3.2:65471142-65471161 None:intergenic 50.0%
CGACTACGACATCATGGAAG+GGG - chr3.2:65471691-65471710 MS.gene08239:CDS 50.0%
CGATGATTGTGTCCATGAGG+TGG + chr3.2:65471139-65471158 None:intergenic 50.0%
GAGAGTGGTTAGGTGAGGTA+GGG - chr3.2:65472281-65472300 MS.gene08239:CDS 50.0%
GAGGTGGTGAAAGTCATCTG+GGG + chr3.2:65471123-65471142 None:intergenic 50.0%
GCATTATCACGGACAGGAAC+CGG - chr3.2:65471779-65471798 MS.gene08239:CDS 50.0%
GGGTATAGGGTTGAAGAGGT+GGG - chr3.2:65472373-65472392 MS.gene08239:CDS 50.0%
GGTTAGGTGAGGTAGGGTTT+AGG - chr3.2:65472287-65472306 MS.gene08239:CDS 50.0%
GTCAACTGGGACACCTTTAC+AGG - chr3.2:65471095-65471114 MS.gene08239:CDS 50.0%
GTTGTCATGGGGACCATTTG+TGG + chr3.2:65471546-65471565 None:intergenic 50.0%
TCAAGCCATCATCGAAGCTG+TGG - chr3.2:65471643-65471662 MS.gene08239:CDS 50.0%
TCACCTTAGTTACCGTGCAC+CGG - chr3.2:65471982-65472001 MS.gene08239:CDS 50.0%
TCCAAAGCTAGTGACGTTGG+TGG - chr3.2:65472045-65472064 MS.gene08239:CDS 50.0%
TGAAGAGGAGAGAAGGTCGT+GGG - chr3.2:65472258-65472277 MS.gene08239:CDS 50.0%
TTCTTCCTCATCGGTGGTTG+TGG + chr3.2:65471219-65471238 None:intergenic 50.0%
! AAGCAACTGATTCCGGTGCA+CGG + chr3.2:65471997-65472016 None:intergenic 50.0%
! AAGCAGCAAGTGTGTCGTTC+TGG + chr3.2:65471568-65471587 None:intergenic 50.0%
! AAGGTGTCCCAGTTGACGAT+GGG + chr3.2:65471092-65471111 None:intergenic 50.0%
! CATGGACTACTAGAAGGTGC+TGG - chr3.2:65471479-65471498 MS.gene08239:CDS 50.0%
! CGTTTTCTTTCCACCACTGC+AGG - chr3.2:65471873-65471892 MS.gene08239:CDS 50.0%
! CTAGTGCACCTATTGATGGC+TGG - chr3.2:65471320-65471339 MS.gene08239:CDS 50.0%
! GCCCTTCATGGACTACTAGA+AGG - chr3.2:65471473-65471492 MS.gene08239:CDS 50.0%
! GGTTCCTGTCCGTGATAATG+CGG + chr3.2:65471780-65471799 None:intergenic 50.0%
! GTGTCGTTCTGGTTGTCATG+GGG + chr3.2:65471557-65471576 None:intergenic 50.0%
! GTGTGTCGTTCTGGTTGTCA+TGG + chr3.2:65471559-65471578 None:intergenic 50.0%
! TCCACCAACGTCACTAGCTT+TGG + chr3.2:65472049-65472068 None:intergenic 50.0%
! TGGCTTGATTGGCTGTGAAG+TGG + chr3.2:65471631-65471650 None:intergenic 50.0%
! TGGTTTTCCGATGCAGAACC+GGG - chr3.2:65472159-65472178 MS.gene08239:CDS 50.0%
!! AAAGGTGTCCCAGTTGACGA+TGG + chr3.2:65471093-65471112 None:intergenic 50.0%
!! CTGGTTTTCCGATGCAGAAC+CGG - chr3.2:65472158-65472177 MS.gene08239:CDS 50.0%
!! GAAGTTGGTTCGGTAGTTGG+TGG - chr3.2:65472073-65472092 MS.gene08239:CDS 50.0%
!! TGGACTACTAGAAGGTGCTG+GGG - chr3.2:65471481-65471500 MS.gene08239:CDS 50.0%
!! TGGTGATGGATGGACTAAGC+TGG + chr3.2:65471184-65471203 None:intergenic 50.0%
!! TTCGGTAGTTGGTGGGTTTG+TGG - chr3.2:65472081-65472100 MS.gene08239:CDS 50.0%
ACTAAGCTGGTCGAGGACAG+TGG + chr3.2:65471171-65471190 None:intergenic 55.0%
AGCTGTGTCCAGAGAAGTGG+AGG + chr3.2:65471010-65471029 None:intergenic 55.0%
AGGGTTGAAGAGGTGGGTGT+GGG - chr3.2:65472379-65472398 MS.gene08239:CDS 55.0%
AGTTGACGATGGGAGACCAG+TGG + chr3.2:65471082-65471101 None:intergenic 55.0%
ATACCCTCTCCACCAAAGCC+CGG + chr3.2:65472186-65472205 None:intergenic 55.0%
ATGGTTGCACCTGGAACCAC+TGG - chr3.2:65471063-65471082 MS.gene08239:CDS 55.0%
CATCTGGGTCTAACCTGCAG+TGG + chr3.2:65471889-65471908 None:intergenic 55.0%
CATGGGGACCATTTGTGGTG+AGG + chr3.2:65471541-65471560 None:intergenic 55.0%
CCTAGGATTGCAGGCTCGTT+GGG - chr3.2:65472214-65472233 MS.gene08239:CDS 55.0%
CGACGGGTATAGGGTTGAAG+AGG - chr3.2:65472369-65472388 MS.gene08239:CDS 55.0%
CGGGTATAGGGTTGAAGAGG+TGG - chr3.2:65472372-65472391 MS.gene08239:CDS 55.0%
CGTAGTCGATGACATGGACG+CGG + chr3.2:65471679-65471698 None:intergenic 55.0%
CTGGTCTCCCATCGTCAACT+GGG - chr3.2:65471082-65471101 MS.gene08239:CDS 55.0%
GAAGAGGAGAGAAGGTCGTG+GGG - chr3.2:65472259-65472278 MS.gene08239:CDS 55.0%
GAAGGAGGTTAATCGCCTGC+CGG + chr3.2:65471834-65471853 None:intergenic 55.0%
GCTTGTACGTGGAGAGGCTT+TGG - chr3.2:65471934-65471953 MS.gene08239:CDS 55.0%
GGAGAGTGGTTAGGTGAGGT+AGG - chr3.2:65472280-65472299 MS.gene08239:CDS 55.0%
GGCCGGATATATAGAACGGG+TGG - chr3.2:65472235-65472254 MS.gene08239:CDS 55.0%
GGTAGGGTTTAGGGGAGTTC+CGG - chr3.2:65472297-65472316 MS.gene08239:CDS 55.0%
GGTGCACGGTAACTAAGGTG+AGG + chr3.2:65471983-65472002 None:intergenic 55.0%
GTGAAGAGGAGAGAAGGTCG+TGG - chr3.2:65472257-65472276 MS.gene08239:CDS 55.0%
TAGGGTTGAAGAGGTGGGTG+TGG - chr3.2:65472378-65472397 MS.gene08239:CDS 55.0%
TTCCTCATCGGTGGTTGTGG+TGG + chr3.2:65471216-65471235 None:intergenic 55.0%
! ACTGGTCTCCCATCGTCAAC+TGG - chr3.2:65471081-65471100 MS.gene08239:CDS 55.0%
! ATCGTCGGAAGTAGGGGTGT+TGG + chr3.2:65471039-65471058 None:intergenic 55.0%
! GTCATCTGGGGCACCTGTAA+AGG + chr3.2:65471111-65471130 None:intergenic 55.0%
!! GGAAGTAGGGGTGTTGGTGA+GGG + chr3.2:65471033-65471052 None:intergenic 55.0%
!! GGACTACTAGAAGGTGCTGG+GGG - chr3.2:65471482-65471501 MS.gene08239:CDS 55.0%
!! GTGGTGGTTGTGGTGATGGA+TGG + chr3.2:65471194-65471213 None:intergenic 55.0%
!! TCGCCTGCCGGTTTCTTGTA+CGG + chr3.2:65471822-65471841 None:intergenic 55.0%
!! TGGATGGACTAAGCTGGTCG+AGG + chr3.2:65471178-65471197 None:intergenic 55.0%
AGAGAAGGTCGTGGGGAGAG+TGG - chr3.2:65472266-65472285 MS.gene08239:CDS 60.0%
AGGTCGTGGGGAGAGTGGTT+AGG - chr3.2:65472271-65472290 MS.gene08239:CDS 60.0%
CACCACCACAACCACCGATG+AGG - chr3.2:65471211-65471230 MS.gene08239:CDS 60.0%
CCACCGTACAAGAAACCGGC+AGG - chr3.2:65471816-65471835 MS.gene08239:CDS 60.0%
CCCAACGAGCCTGCAATCCT+AGG + chr3.2:65472217-65472236 None:intergenic 60.0%
CGGGTGGTGAAGAGGAGAGA+AGG - chr3.2:65472251-65472270 MS.gene08239:CDS 60.0%
CTCATCGGTGGTTGTGGTGG+TGG + chr3.2:65471213-65471232 None:intergenic 60.0%
CTGGGTCTAACCTGCAGTGG+TGG + chr3.2:65471886-65471905 None:intergenic 60.0%
CTTCCGCGCCAGCCATCAAT+AGG + chr3.2:65471331-65471350 None:intergenic 60.0%
GATGGGAGACCAGTGGTTCC+AGG + chr3.2:65471075-65471094 None:intergenic 60.0%
GCCTAGGATTGCAGGCTCGT+TGG - chr3.2:65472213-65472232 MS.gene08239:CDS 60.0%
GGGAGCTGTGTCCAGAGAAG+TGG + chr3.2:65471013-65471032 None:intergenic 60.0%
GTACGGTGGCGATTGACCTC+CGG + chr3.2:65471805-65471824 None:intergenic 60.0%
GTGGGCCACAGCTTCGATGA+TGG + chr3.2:65471651-65471670 None:intergenic 60.0%
GTGGGGAGAGTGGTTAGGTG+AGG - chr3.2:65472276-65472295 MS.gene08239:CDS 60.0%
TACCCTCTCCACCAAAGCCC+GGG + chr3.2:65472185-65472204 None:intergenic 60.0%
TATCACGGACAGGAACCGGC+CGG - chr3.2:65471783-65471802 MS.gene08239:CDS 60.0%
TTGTCCAAGGGAAGCGGCGA+AGG + chr3.2:65471852-65471871 None:intergenic 60.0%
! GCACCTATTGATGGCTGGCG+CGG - chr3.2:65471325-65471344 MS.gene08239:CDS 60.0%
!! ATCGGTGGTTGTGGTGGTGG+TGG + chr3.2:65471210-65471229 None:intergenic 60.0%
!! CCTGCCGGTTTCTTGTACGG+TGG + chr3.2:65471819-65471838 None:intergenic 60.0%
!! CGGAAGTAGGGGTGTTGGTG+AGG + chr3.2:65471034-65471053 None:intergenic 60.0%
!! GGTGGTGGTGGTTGTGGTGA+TGG + chr3.2:65471198-65471217 None:intergenic 60.0%
!! GGTTGTGGTGGTGGTGGTTG+TGG + chr3.2:65471204-65471223 None:intergenic 60.0%
ACCTCCTTCGCCGCTTCCCT+TGG - chr3.2:65471845-65471864 MS.gene08239:CDS 65.0%
GCTGGCGCGGAAGCCTTAAC+CGG - chr3.2:65471338-65471357 MS.gene08239:CDS 65.0%
GGATTGCAGGCTCGTTGGGC+CGG - chr3.2:65472218-65472237 MS.gene08239:CDS 65.0%
TCCAAGGGAAGCGGCGAAGG+AGG + chr3.2:65471849-65471868 None:intergenic 65.0%
TTCCGATGCAGAACCGGGCC+CGG - chr3.2:65472164-65472183 MS.gene08239:CDS 65.0%
GACATGGACGCGGCGCTCGT+GGG + chr3.2:65471669-65471688 None:intergenic 70.0%
GGTGGCGATTGACCTCCGGC+CGG + chr3.2:65471801-65471820 None:intergenic 70.0%
TCCGATGCAGAACCGGGCCC+GGG - chr3.2:65472165-65472184 MS.gene08239:CDS 70.0%
TCTCCACCAAAGCCCGGGCC+CGG + chr3.2:65472180-65472199 None:intergenic 70.0%
TGACATGGACGCGGCGCTCG+TGG + chr3.2:65471670-65471689 None:intergenic 70.0%
! GGCCCGGGCTTTGGTGGAGA+GGG - chr3.2:65472180-65472199 MS.gene08239:CDS 70.0%
CACGGACAGGAACCGGCCGG+AGG - chr3.2:65471786-65471805 MS.gene08239:CDS 75.0%
GCAGAACCGGGCCCGGGCTT+TGG - chr3.2:65472171-65472190 MS.gene08239:CDS 75.0%
GCCCGGGCCCGGTTCTGCAT+CGG + chr3.2:65472169-65472188 None:intergenic 75.0%
! GAACCGGGCCCGGGCTTTGG+TGG - chr3.2:65472174-65472193 MS.gene08239:CDS 75.0%
! GGGCCCGGGCTTTGGTGGAG+AGG - chr3.2:65472179-65472198 MS.gene08239:CDS 75.0%


Chromosome Type Strat End Strand Name
chr3.2 gene 65470975 65472492 65470975 ID=MS.gene08239
chr3.2 mRNA 65470975 65472492 65470975 ID=MS.gene08239.t1;Parent=MS.gene08239
chr3.2 exon 65470975 65472492 65470975 ID=MS.gene08239.t1.exon1;Parent=MS.gene08239.t1
chr3.2 CDS 65470975 65472492 65470975 ID=cds.MS.gene08239.t1;Parent=MS.gene08239.t1
Gene Sequence

>MS.gene08239

ATGGACATGATGGACATGGATGCCATCAATGACCTCCACTTCTCTGGACACAGCTCCCTCACCAACACCCCTACTTCCGACGATGATTATGGTTGCACCTGGAACCACTGGTCTCCCATCGTCAACTGGGACACCTTTACAGGTGCCCCAGATGACTTTCACCACCTCATGGACACAATCATCGAGGACAGAACCACTGTCCTCGACCAGCTTAGTCCATCCATCACCACAACCACCACCACCACAACCACCGATGAGGAAGAAGAAGAAATGGAAACAACAACCACAACAACAACAACGACAGAAAAAACACAAGAAGTTGGTGATGATTCAAAAGGGCTAAAACTAGTGCACCTATTGATGGCTGGCGCGGAAGCCTTAACCGGTTCAACCAAAAACCGTGACTTAGCTCGAGTGATATTGATCCGGCTCAAGGAATTAGTCTCACAACATGCTAACGGCTCTAACATGGAGAGACTCGCCGCTCATTTCACCGAAGCCCTTCATGGACTACTAGAAGGTGCTGGGGGTGCGCACAACAACCACCACAACAACAAACATTACCTCACCACAAATGGTCCCCATGACAACCAGAACGACACACTTGCTGCTTTCCAACTACTTCAAGACATGTCACCTTACGTCAAATTCGGCCACTTCACAGCCAATCAAGCCATCATCGAAGCTGTGGCCCACGAGCGCCGCGTCCATGTCATCGACTACGACATCATGGAAGGGGTCCAATGGGCTTCATTAATCCAATCACTAGCTTCCAACAACAACGGTCCTCATCTTCGGATAACCGCATTATCACGGACAGGAACCGGCCGGAGGTCAATCGCCACCGTACAAGAAACCGGCAGGCGATTAACCTCCTTCGCCGCTTCCCTTGGACAACCGTTTTCTTTCCACCACTGCAGGTTAGACCCAGATGAAACTTTCCGTCCTTCTGCATTAAAGCTTGTACGTGGAGAGGCTTTGGTTTTCAACTGCATGCTGAATTTACCTCACCTTAGTTACCGTGCACCGGAATCAGTTGCTTCATTCTTGAACGGAGCAAAAACGTTGAATCCAAAGCTAGTGACGTTGGTGGAAGAAGAAGTTGGTTCGGTAGTTGGTGGGTTTGTGGAAAGGTTCATGGACTCACTTCATCACTATTCAGCGGTTTTTGATTCATTGGAGGCTGGTTTTCCGATGCAGAACCGGGCCCGGGCTTTGGTGGAGAGGGTATTTTTTGGGCCTAGGATTGCAGGCTCGTTGGGCCGGATATATAGAACGGGTGGTGAAGAGGAGAGAAGGTCGTGGGGAGAGTGGTTAGGTGAGGTAGGGTTTAGGGGAGTTCCGGTGAGCTTTGCAAATCATTGTCAAGCAAAGCTGTTGTTAGGGCTTTTTAACGACGGGTATAGGGTTGAAGAGGTGGGTGTGGGTAGTAATAAGTTGGTGTTGGATTGGAAATCAAGGCGTTTGCTTTCTGCTTCCCTTTGGACTTGTTCTTCTTCTTCAGATTCTGATTTATAG
Protein sequence

>MS.gene08239.t1

MDMMDMDAINDLHFSGHSSLTNTPTSDDDYGCTWNHWSPIVNWDTFTGAPDDFHHLMDTIIEDRTTVLDQLSPSITTTTTTTTTDEEEEEMETTTTTTTTTEKTQEVGDDSKGLKLVHLLMAGAEALTGSTKNRDLARVILIRLKELVSQHANGSNMERLAAHFTEALHGLLEGAGGAHNNHHNNKHYLTTNGPHDNQNDTLAAFQLLQDMSPYVKFGHFTANQAIIEAVAHERRVHVIDYDIMEGVQWASLIQSLASNNNGPHLRITALSRTGTGRRSIATVQETGRRLTSFAASLGQPFSFHHCRLDPDETFRPSALKLVRGEALVFNCMLNLPHLSYRAPESVASFLNGAKTLNPKLVTLVEEEVGSVVGGFVERFMDSLHHYSAVFDSLEAGFPMQNRARALVERVFFGPRIAGSLGRIYRTGGEEERRSWGEWLGEVGFRGVPVSFANHCQAKLLLGLFNDGYRVEEVGVGSNKLVLDWKSRRLLSASLWTCSSSSDSDL