AlfalfaGEDB | MS.gene08335

M. sativa cultivar XinJiangDaYe / MS.gene08335

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08335.t1	RHN42358.1	97.7	217	5	0	1	217	1	217	8.80E-115	422.9

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08335.t1	Q39263	46.2	182	68	7	62	217	83	260	5.7e-30	132.5

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08335.t1	A0A396GPM5	97.7	217	5	0	1	217	1	217	6.3e-115	422.9

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene08335.t1	TF	C2H2

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08335.t1	MTR_1g033340	52.157	255	79	8	1	217	2	251	9.33e-79	236
MS.gene08335.t1	MTR_3g065020	48.500	200	92	6	21	217	47	238	2.18e-57	182
MS.gene08335.t1	MTR_5g077370	46.078	204	91	7	23	217	44	237	2.27e-49	161
MS.gene08335.t1	MTR_2g104900	66.667	81	21	2	59	133	78	158	6.57e-30	111
MS.gene08335.t1	MTR_1g013130	40.972	144	59	4	15	132	37	180	6.60e-25	98.6
MS.gene08335.t1	MTR_1g107055	45.763	118	58	3	62	179	64	175	7.38e-25	97.1
MS.gene08335.t1	MTR_4g006575	40.146	137	64	4	2	132	6	130	8.48e-23	92.4
MS.gene08335.t1	MTR_4g006600	40.146	137	64	4	2	132	6	130	8.48e-23	92.4
MS.gene08335.t1	MTR_3g106230	50.562	89	26	4	61	132	71	158	1.07e-19	84.3
MS.gene08335.t1	MTR_4g128340	62.295	61	21	1	59	119	42	100	4.80e-18	78.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene08335.t1	AT1G66140	46.524	187	70	7	57	217	78	260	1.85e-44	149
MS.gene08335.t1	AT1G24625	39.450	218	90	8	12	217	22	209	2.11e-33	119
MS.gene08335.t1	AT1G80730	55.056	89	23	2	61	132	65	153	1.55e-25	99.8
MS.gene08335.t1	AT5G25160	53.012	83	34	2	55	132	52	134	1.76e-22	91.7
MS.gene08335.t1	AT5G10970	42.105	133	67	5	9	132	44	175	4.29e-22	91.7
MS.gene08335.t1	AT5G57520	76.923	39	9	0	61	99	49	87	1.10e-16	74.3
MS.gene08335.t1	AT5G14010	72.222	36	10	0	62	97	36	71	3.69e-13	65.1
MS.gene08335.t1	AT5G01860	57.500	40	17	0	62	101	65	104	2.24e-11	61.2

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Find 55 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACCCGAATTTGAAGTTTG+AGG	0.449808	4.1:-16857634	None:intergenic
AAATTTGATAGAAACCATTT+TGG	0.216847	4.1:+16857517	MS.gene08335:CDS
AATATCCTTGGAGTTACCGA+TGG	0.642699	4.1:-16857235	None:intergenic
AGAACGCTCACAAAAGGGAG+AGG	0.635123	4.1:+16857314	MS.gene08335:CDS
AGAATGAGCTTCAACACCAA+GGG	0.655962	4.1:-16857428	None:intergenic
AGTGATGCTAGTAGTGAAGT+AGG	0.485652	4.1:+16857190	MS.gene08335:CDS
AGTGTCAAGTCAAGGGTCAT+AGG	0.518887	4.1:-16857130	None:intergenic
ATATCCTCAAACTTCAAATT+CGG	0.284335	4.1:+16857630	MS.gene08335:CDS
ATCACTTGAACCCATTAACT+CGG	0.620524	4.1:-16857173	None:intergenic
ATGATGATGTAGGTAGCTTT+TGG	0.366343	4.1:+16857560	MS.gene08335:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TATCCTCAAACTTCAAATTC+GGG	+	chr4.1:16857631-16857650	MS.gene08335:CDS	30.0%
ATCACTTGAACCCATTAACT+CGG	-	chr4.1:16857176-16857195	None:intergenic	35.0%
CTATATTAACACTTCCTCCT+TGG	-	chr4.1:16857609-16857628	None:intergenic	35.0%
GAAATTGAGTGTCAAGTCAA+GGG	-	chr4.1:16857140-16857159	None:intergenic	35.0%
TATAGCTTCAGTGTAAGATC+AGG	-	chr4.1:16857700-16857719	None:intergenic	35.0%
TCTCTTGTAATTACTGCAAG+AGG	+	chr4.1:16857257-16857276	MS.gene08335:CDS	35.0%
TGAAATTGAGTGTCAAGTCA+AGG	-	chr4.1:16857141-16857160	None:intergenic	35.0%
AATATCCTTGGAGTTACCGA+TGG	-	chr4.1:16857238-16857257	None:intergenic	40.0%
AGAATGAGCTTCAACACCAA+GGG	-	chr4.1:16857431-16857450	None:intergenic	40.0%
AGTGATGCTAGTAGTGAAGT+AGG	+	chr4.1:16857190-16857209	MS.gene08335:CDS	40.0%

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Chromosome	Type	Strat	End	Strand	Name
chr4.1	gene	16857067	16857720	16857067	ID=MS.gene08335
chr4.1	mRNA	16857067	16857720	16857067	ID=MS.gene08335.t1;Parent=MS.gene08335
chr4.1	exon	16857067	16857720	16857067	ID=MS.gene08335.t1.exon1;Parent=MS.gene08335.t1
chr4.1	CDS	16857067	16857720	16857067	ID=cds.MS.gene08335.t1;Parent=MS.gene08335.t1

Gene Sequence

>MS.gene08335

ATGATGACTCCAAACTTGAACTTGGAACCTGAAAATCAGTCAGAAGCACCCCAAGATCTAGATCCTATGACCCTTGACTTGACACTCAATTTCAGTTCGAGTGACGCCGAGTTAATGGGTTCAAGTGATGCTAGTAGTGAAGTAGGAGCTGCTGAAGTTCATGCTTCGCCATCGGTAACTCCAAGGATATTCTCTTGTAATTACTGCAAGAGGAAGTTCTATAGCTCGCAAGCATTAGGCGGTCATCAGAACGCTCACAAAAGGGAGAGGACGATGGCTAAACGTGCAATGCGAATGGGAATGTTCACCGAAAGGTATACAAGCCTTGCATCTCTACCTCTTCATGGTTCGCCTTTTCGGTCCCTTGGTGTTGAAGCTCATTCTGCAATGCATCAAAGACATATGCAACCATCGTCAACAATGAGGGCTCCAGATATGAGAGCTGCTGCGAAATTTGATAGAAACCATTTTGGATCGCTAGTTTTCGTGGAAGATGATGATGTAGGTAGCTTTTGGCCCGGAAGTTTTAGACAGGTGGACCAAGGAGGAAGTGTTAATATAGGATATCCTCAAACTTCAAATTCGGGTTTTGTTCCAATGGCACCTCCTCCACCTCAAGCATCTGCATCTCCTGATCTTACACTGAAGCTATAA

Protein sequence

>MS.gene08335.t1

MMTPNLNLEPENQSEAPQDLDPMTLDLTLNFSSSDAELMGSSDASSEVGAAEVHASPSVTPRIFSCNYCKRKFYSSQALGGHQNAHKRERTMAKRAMRMGMFTERYTSLASLPLHGSPFRSLGVEAHSAMHQRHMQPSSTMRAPDMRAAAKFDRNHFGSLVFVEDDDVGSFWPGSFRQVDQGGSVNIGYPQTSNSGFVPMAPPPPQASASPDLTLKL