Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08381.t1 | PNX80761.1 | 47.8 | 115 | 51 | 4 | 51 | 164 | 17 | 123 | 1.70E-15 | 92.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08381.t1 | A0A2K3LQE0 | 47.8 | 115 | 51 | 4 | 51 | 164 | 17 | 123 | 1.2e-15 | 92.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene08381.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene08381.t1 | MTR_3g080590 | 59.200 | 125 | 47 | 3 | 7 | 130 | 31 | 152 | 3.60e-35 | 121 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 21 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAACGTGCAGGAGCTAATTC+AGG | 0.337163 | 4.1:-17385086 | None:intergenic |
| GTCTACGACTTCGTAAGAAT+TGG | 0.410779 | 4.1:+17385033 | MS.gene08381:CDS |
| TTCGAATGGTTGTCCTAGAT+TGG | 0.412474 | 4.1:-17384984 | None:intergenic |
| CACTATGCATTTGTAACCAT+TGG | 0.416660 | 4.1:-17384759 | None:intergenic |
| TGAAGAATGACAACCAATCT+AGG | 0.416853 | 4.1:+17384971 | MS.gene08381:CDS |
| AAACATTACATAAGACTTTG+TGG | 0.418547 | 4.1:-17384836 | None:intergenic |
| TTTCACTTCATTCTTTCTTA+CGG | 0.439411 | 4.1:-17384878 | None:intergenic |
| CGCACAACAATTATTTCGAA+TGG | 0.477990 | 4.1:-17384998 | None:intergenic |
| CAATTAGTATGCAATACATA+TGG | 0.480421 | 4.1:+17384928 | MS.gene08381:CDS |
| TGACAGATGATGATGATGTA+AGG | 0.507309 | 4.1:+17384785 | MS.gene08381:CDS |
| AATACATATGGTGTTAATGA+TGG | 0.546002 | 4.1:+17384940 | MS.gene08381:CDS |
| CGTACTGATAGAGATGTAGA+TGG | 0.548996 | 4.1:+17384682 | MS.gene08381:CDS |
| GTGGAATTCAACCATGGTAA+TGG | 0.562978 | 4.1:+17384706 | MS.gene08381:CDS |
| AACGTGCAGGAGCTAATTCA+GGG | 0.566580 | 4.1:-17385085 | None:intergenic |
| AGCTAATTCAGGGTTGTGAG+AGG | 0.581901 | 4.1:-17385075 | None:intergenic |
| GCTAATTCAGGGTTGTGAGA+GGG | 0.583485 | 4.1:-17385074 | None:intergenic |
| GCTTCAGTATCACTATCTAT+CGG | 0.596147 | 4.1:-17384562 | None:intergenic |
| GCTTCAGTATCACTATCTAT+CGG | 0.596147 | 4.1:-17384631 | None:intergenic |
| AATGGTGTTTGCAGTGAACA+AGG | 0.604546 | 4.1:+17384724 | MS.gene08381:CDS |
| TGATAGAGATGTAGATGGTG+TGG | 0.606468 | 4.1:+17384687 | MS.gene08381:CDS |
| CACTGCAAACACCATTACCA+TGG | 0.617401 | 4.1:-17384717 | None:intergenic |
| GACAGATGATGATGATGTAA+GGG | 0.635954 | 4.1:+17384786 | MS.gene08381:CDS |
| AAGGGAAAGAGAAACTCCAA+TGG | 0.674525 | 4.1:+17384743 | MS.gene08381:CDS |
| GGAAATGAAATTCAACACAG+TGG | 0.694737 | 4.1:+17384807 | MS.gene08381:CDS |
| ATGGTGTTTGCAGTGAACAA+GGG | 0.699949 | 4.1:+17384725 | MS.gene08381:CDS |
| GATGGTGTGGAATTCAACCA+TGG | 0.744654 | 4.1:+17384700 | MS.gene08381:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAACATTACATAAGACTTTG+TGG | - | chr4.1:17384839-17384858 | None:intergenic | 25.0% |
| ! | CAATTAGTATGCAATACATA+TGG | + | chr4.1:17384928-17384947 | MS.gene08381:CDS | 25.0% |
| ! | TTTCACTTCATTCTTTCTTA+CGG | - | chr4.1:17384881-17384900 | None:intergenic | 25.0% |
| !! | AATACATATGGTGTTAATGA+TGG | + | chr4.1:17384940-17384959 | MS.gene08381:CDS | 25.0% |
| CACTATGCATTTGTAACCAT+TGG | - | chr4.1:17384762-17384781 | None:intergenic | 35.0% | |
| CGCACAACAATTATTTCGAA+TGG | - | chr4.1:17385001-17385020 | None:intergenic | 35.0% | |
| GCTTCAGTATCACTATCTAT+CGG | - | chr4.1:17384565-17384584 | None:intergenic | 35.0% | |
| GGAAATGAAATTCAACACAG+TGG | + | chr4.1:17384807-17384826 | MS.gene08381:CDS | 35.0% | |
| TGAAGAATGACAACCAATCT+AGG | + | chr4.1:17384971-17384990 | MS.gene08381:CDS | 35.0% | |
| ! | GACAGATGATGATGATGTAA+GGG | + | chr4.1:17384786-17384805 | MS.gene08381:CDS | 35.0% |
| ! | TGACAGATGATGATGATGTA+AGG | + | chr4.1:17384785-17384804 | MS.gene08381:CDS | 35.0% |
| AAGGGAAAGAGAAACTCCAA+TGG | + | chr4.1:17384743-17384762 | MS.gene08381:CDS | 40.0% | |
| AATGGTGTTTGCAGTGAACA+AGG | + | chr4.1:17384724-17384743 | MS.gene08381:CDS | 40.0% | |
| ATGGTGTTTGCAGTGAACAA+GGG | + | chr4.1:17384725-17384744 | MS.gene08381:CDS | 40.0% | |
| GTCTACGACTTCGTAAGAAT+TGG | + | chr4.1:17385033-17385052 | MS.gene08381:CDS | 40.0% | |
| GTGGAATTCAACCATGGTAA+TGG | + | chr4.1:17384706-17384725 | MS.gene08381:CDS | 40.0% | |
| TGATAGAGATGTAGATGGTG+TGG | + | chr4.1:17384687-17384706 | MS.gene08381:CDS | 40.0% | |
| TTCGAATGGTTGTCCTAGAT+TGG | - | chr4.1:17384987-17385006 | None:intergenic | 40.0% | |
| ! | CGTACTGATAGAGATGTAGA+TGG | + | chr4.1:17384682-17384701 | MS.gene08381:CDS | 40.0% |
| CACTGCAAACACCATTACCA+TGG | - | chr4.1:17384720-17384739 | None:intergenic | 45.0% | |
| GATGGTGTGGAATTCAACCA+TGG | + | chr4.1:17384700-17384719 | MS.gene08381:CDS | 45.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 17384550 | 17385095 | 17384550 | ID=MS.gene08381 |
| chr4.1 | mRNA | 17384550 | 17385095 | 17384550 | ID=MS.gene08381.t1;Parent=MS.gene08381 |
| chr4.1 | exon | 17384550 | 17385095 | 17384550 | ID=MS.gene08381.t1.exon1;Parent=MS.gene08381.t1 |
| chr4.1 | CDS | 17384550 | 17385095 | 17384550 | ID=cds.MS.gene08381.t1;Parent=MS.gene08381.t1 |
| Gene Sequence |
| Protein sequence |
