AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene08382

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08382.t1 MTR_3g080590 63.871 155 33 3 1 132 1 155 3.66e-59 182
MS.gene08382.t1 MTR_8g035530 41.667 72 40 2 72 141 2 73 1.89e-11 57.8
MS.gene08382.t1 MTR_7g028855 37.864 103 55 3 78 173 115 215 3.94e-11 61.2
MS.gene08382.t1 MTR_7g446520 40.000 75 42 2 78 151 83 155 4.03e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 26 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACAACTTTGTTTGTAAGAAT+TGG 0.289038 4.1:-17387762 MS.gene08382:CDS
GTGAAGATATAGAAGTCAAT+AGG 0.343295 4.1:-17388143 MS.gene08382:CDS
TGTACATAGTGATATAAATA+AGG 0.389788 4.1:-17388208 MS.gene08382:CDS
TGAAGATATAGAAGTCAATA+GGG 0.403531 4.1:-17388142 MS.gene08382:CDS
ATAAGAATGGCAGCCGATCT+AGG 0.405495 4.1:-17387828 MS.gene08382:CDS
AACGCGCTAGAGCTAATTCA+TGG 0.466898 4.1:+17387711 None:intergenic
CGCACAACAGTTAGTTCGAA+TGG 0.478548 4.1:+17387801 None:intergenic
TTCGAATGGTTGTCCTAGAT+CGG 0.479891 4.1:+17387815 None:intergenic
GTGATGTGACAGATGATGTA+AGG 0.502812 4.1:-17388011 MS.gene08382:CDS
TCAACTAGTATGCAATAAAG+AGG 0.542921 4.1:-17387869 MS.gene08382:CDS
ATGTAATGTTTGCGAGACAC+AGG 0.561187 4.1:-17387948 MS.gene08382:CDS
GTGGAATTCAACCATGGTAA+TGG 0.562978 4.1:-17388084 MS.gene08382:CDS
ACATAGTGATATAAATAAGG+TGG 0.582699 4.1:-17388205 MS.gene08382:CDS
ACTGATGCATTTGTAACCAT+CGG 0.601265 4.1:+17388032 None:intergenic
AATGGTGTTTGCAGTGAGCA+AGG 0.608644 4.1:-17388066 MS.gene08382:CDS
ATGGTGTTTGCAGTGAGCAA+GGG 0.627698 4.1:-17388065 MS.gene08382:CDS
ATATCACTATGTACATCAAG+AGG 0.628809 4.1:+17388216 None:intergenic
CAACTAGTATGCAATAAAGA+GGG 0.632289 4.1:-17387868 MS.gene08382:CDS
TAATAGAGATGCAGACGGTG+TGG 0.636745 4.1:-17388103 MS.gene08382:CDS
CACTGCAAACACCATTACCA+TGG 0.645481 4.1:+17388073 None:intergenic
CAAGGGAAAGAGAACTCCGA+TGG 0.649967 4.1:-17388048 MS.gene08382:CDS
GAAGATATAGAAGTCAATAG+GGG 0.650650 4.1:-17388141 MS.gene08382:CDS
TGATGTGACAGATGATGTAA+GGG 0.654823 4.1:-17388010 MS.gene08382:CDS
GACGGTGTGGAATTCAACCA+TGG 0.691057 4.1:-17388090 MS.gene08382:CDS
CGTACTAATAGAGATGCAGA+CGG 0.718664 4.1:-17388108 MS.gene08382:CDS
GGATATAGAATTTAACACAG+AGG 0.735756 4.1:-17387989 MS.gene08382:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGATGAAAAAAATAAGAA+TGG - chr4.1:17388125-17388144 MS.gene08382:CDS 15.0%
!! TGTACATAGTGATATAAATA+AGG - chr4.1:17387758-17387777 MS.gene08382:CDS 20.0%
! ACATAGTGATATAAATAAGG+TGG - chr4.1:17387761-17387780 MS.gene08382:CDS 25.0%
! TGAAGATATAGAAGTCAATA+GGG - chr4.1:17387824-17387843 MS.gene08382:CDS 25.0%
!! ACAACTTTGTTTGTAAGAAT+TGG - chr4.1:17388204-17388223 MS.gene08382:CDS 25.0%
!! TTTCACTTCATCTTTTCTTA+CGG + chr4.1:17388048-17388067 None:intergenic 25.0%
ATATCACTATGTACATCAAG+AGG + chr4.1:17387753-17387772 None:intergenic 30.0%
CAACTAGTATGCAATAAAGA+GGG - chr4.1:17388098-17388117 MS.gene08382:CDS 30.0%
GAAGATATAGAAGTCAATAG+GGG - chr4.1:17387825-17387844 MS.gene08382:CDS 30.0%
GGATATAGAATTTAACACAG+AGG - chr4.1:17387977-17387996 MS.gene08382:CDS 30.0%
GTGAAGATATAGAAGTCAAT+AGG - chr4.1:17387823-17387842 MS.gene08382:CDS 30.0%
TCAACTAGTATGCAATAAAG+AGG - chr4.1:17388097-17388116 MS.gene08382:CDS 30.0%
ACTGATGCATTTGTAACCAT+CGG + chr4.1:17387937-17387956 None:intergenic 35.0%
TGATGTGACAGATGATGTAA+GGG - chr4.1:17387956-17387975 MS.gene08382:CDS 35.0%
ATGTAATGTTTGCGAGACAC+AGG - chr4.1:17388018-17388037 MS.gene08382:CDS 40.0%
CGTACTAATAGAGATGCAGA+CGG - chr4.1:17387858-17387877 MS.gene08382:CDS 40.0%
GTGATGTGACAGATGATGTA+AGG - chr4.1:17387955-17387974 MS.gene08382:CDS 40.0%
GTGGAATTCAACCATGGTAA+TGG - chr4.1:17387882-17387901 MS.gene08382:CDS 40.0%
TTCGAATGGTTGTCCTAGAT+CGG + chr4.1:17388154-17388173 None:intergenic 40.0%
AATGGTGTTTGCAGTGAGCA+AGG - chr4.1:17387900-17387919 MS.gene08382:CDS 45.0%
ATAAGAATGGCAGCCGATCT+AGG - chr4.1:17388138-17388157 MS.gene08382:CDS 45.0%
ATGGTGTTTGCAGTGAGCAA+GGG - chr4.1:17387901-17387920 MS.gene08382:CDS 45.0%
CACTGCAAACACCATTACCA+TGG + chr4.1:17387896-17387915 None:intergenic 45.0%
CGCACAACAGTTAGTTCGAA+TGG + chr4.1:17388168-17388187 None:intergenic 45.0%
TAATAGAGATGCAGACGGTG+TGG - chr4.1:17387863-17387882 MS.gene08382:CDS 45.0%
CAAGGGAAAGAGAACTCCGA+TGG - chr4.1:17387918-17387937 MS.gene08382:CDS 50.0%
GACGGTGTGGAATTCAACCA+TGG - chr4.1:17387876-17387895 MS.gene08382:CDS 50.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 17387723 17388265 17387723 ID=MS.gene08382
chr4.1 mRNA 17387723 17388265 17387723 ID=MS.gene08382.t1;Parent=MS.gene08382
chr4.1 exon 17387723 17388265 17387723 ID=MS.gene08382.t1.exon1;Parent=MS.gene08382.t1
chr4.1 CDS 17387723 17388265 17387723 ID=cds.MS.gene08382.t1;Parent=MS.gene08382.t1
Gene Sequence

>MS.gene08382

ATGTTTACATATAGTGAAGCAATGTCGCCTCTTGATGTACATAGTGATATAAATAAGGTGGAATTTAATAGTTTTAATGATGTTGACACTGATAGTGATAGTGAAGATATAGAAGTCAATAGGGGCAACAATGTTCGTACTAATAGAGATGCAGACGGTGTGGAATTCAACCATGGTAATGGTGTTTGCAGTGAGCAAGGGAAAGAGAACTCCGATGGTTACAAATGCATCAGTGATGTGACAGATGATGTAAGGGATATAGAATTTAACACAGAGGAAGAAGCTACAGAGTTTTATGTAATGTTTGCGAGACACAGGTTTGCCGTAAGAAAAGATGAAGTGAAACGTGATTCTAATGAGAACATTATTATGCGTCAACTAGTATGCAATAAAGAGGGTGTTAATGATGAAAAAAATAAGAATGGCAGCCGATCTAGGACAACCATTCGAACTAACTGTTGTGCGAAACTTCGAGTAGTCTACAACTTTGTTTGTAAGAATTGGAGAGTTTCTATTTCAAACACTCTCACAACCATGAATTAG
Protein sequence

>MS.gene08382.t1

MFTYSEAMSPLDVHSDINKVEFNSFNDVDTDSDSEDIEVNRGNNVRTNRDADGVEFNHGNGVCSEQGKENSDGYKCISDVTDDVRDIEFNTEEEATEFYVMFARHRFAVRKDEVKRDSNENIIMRQLVCNKEGVNDEKNKNGSRSRTTIRTNCCAKLRVVYNFVCKNWRVSISNTLTTMN