AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene08539

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08539.t1 MTR_8g080690 84.574 376 24 2 1 342 1 376 0.0 582
MS.gene08539.t1 MTR_8g080680 62.269 379 85 7 1 342 1 358 4.24e-150 428
MS.gene08539.t1 MTR_8g080670 56.436 404 98 8 1 342 1 388 2.59e-122 358
MS.gene08539.t1 MTR_3g085180 45.780 391 155 9 1 341 1 384 2.23e-91 280
MS.gene08539.t1 MTR_1g053715 37.945 253 119 7 105 322 25 274 6.00e-37 136
MS.gene08539.t1 MTR_7g092930 40.201 199 85 6 144 314 61 253 6.97e-33 125
MS.gene08539.t1 MTR_3g099080 52.252 111 52 1 151 261 98 207 1.95e-32 124
MS.gene08539.t1 MTR_8g463140 66.250 80 27 0 149 228 63 142 6.02e-32 118
MS.gene08539.t1 MTR_8g096780 39.796 196 100 6 137 328 85 266 6.04e-31 120
MS.gene08539.t1 MTR_2g078770 65.789 76 26 0 149 224 56 131 2.31e-30 114
MS.gene08539.t1 MTR_7g444860 46.405 153 70 4 99 242 98 247 6.74e-30 116
MS.gene08539.t1 MTR_4g109770 40.437 183 83 6 151 326 123 286 1.51e-29 118
MS.gene08539.t1 MTR_5g046670 56.977 86 37 0 149 234 242 327 3.30e-28 114
MS.gene08539.t1 MTR_7g028740 45.614 114 61 1 151 264 77 189 4.92e-28 112
MS.gene08539.t1 MTR_8g005960 64.103 78 28 0 149 226 185 262 9.57e-28 110
MS.gene08539.t1 MTR_4g088555 63.158 76 28 0 149 224 61 136 1.04e-27 106
MS.gene08539.t1 MTR_2g461920 55.556 90 40 0 137 226 157 246 1.08e-27 113
MS.gene08539.t1 MTR_1g035010 57.831 83 35 0 148 230 101 183 5.92e-27 112
MS.gene08539.t1 MTR_2g014200 56.250 80 35 0 149 228 62 141 7.90e-26 100
MS.gene08539.t1 MTR_1g086250 48.718 117 55 1 119 230 110 226 1.08e-25 108
MS.gene08539.t1 MTR_2g046550 58.974 78 32 0 151 228 139 216 1.38e-24 105
MS.gene08539.t1 MTR_7g110320 55.294 85 38 0 151 235 145 229 9.97e-24 102
MS.gene08539.t1 MTR_7g110320 55.294 85 38 0 151 235 145 229 1.13e-23 102
MS.gene08539.t1 MTR_2g020620 39.496 119 58 2 158 269 97 208 2.35e-18 86.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08539.t1 AT5G43270 38.281 384 175 16 1 333 1 373 1.46e-48 168
MS.gene08539.t1 AT5G43270 38.281 384 175 16 1 333 1 373 1.46e-48 168
MS.gene08539.t1 AT5G43270 38.281 384 175 16 1 333 1 373 1.46e-48 168
MS.gene08539.t1 AT1G27360 43.825 251 115 8 1 226 1 250 2.27e-47 165
MS.gene08539.t1 AT1G27360 43.825 251 115 8 1 226 1 250 2.27e-47 165
MS.gene08539.t1 AT1G27360 43.825 251 115 8 1 226 1 250 2.27e-47 165
MS.gene08539.t1 AT1G27360 43.825 251 115 8 1 226 1 250 2.27e-47 165
MS.gene08539.t1 AT1G27370 41.353 266 129 6 1 240 1 265 1.63e-45 159
MS.gene08539.t1 AT1G27370 41.353 266 129 6 1 240 1 265 9.41e-45 158
MS.gene08539.t1 AT1G27370 41.353 266 129 6 1 240 1 265 9.41e-45 158
MS.gene08539.t1 AT1G27370 41.353 266 129 6 1 240 1 265 9.41e-45 158
MS.gene08539.t1 AT1G27370 41.353 266 129 6 1 240 1 265 9.41e-45 158
MS.gene08539.t1 AT1G27370 41.353 266 129 6 1 240 1 265 9.41e-45 158
MS.gene08539.t1 AT1G27370 41.353 266 129 6 1 240 1 265 9.41e-45 158
MS.gene08539.t1 AT2G42200 54.955 111 45 2 148 258 71 176 7.77e-33 125
MS.gene08539.t1 AT1G69170 64.103 78 28 0 147 224 120 197 1.83e-29 117
MS.gene08539.t1 AT5G50670 57.143 84 36 0 147 230 97 180 1.84e-28 113
MS.gene08539.t1 AT5G50670 57.143 84 36 0 147 230 97 180 1.84e-28 113
MS.gene08539.t1 AT5G50570 57.143 84 36 0 147 230 97 180 1.84e-28 113
MS.gene08539.t1 AT5G50570 57.143 84 36 0 147 230 97 180 1.84e-28 113
MS.gene08539.t1 AT1G76580 55.914 93 41 0 148 240 111 203 2.99e-28 115
MS.gene08539.t1 AT1G76580 55.914 93 41 0 148 240 111 203 3.01e-28 115
MS.gene08539.t1 AT1G76580 55.914 93 41 0 148 240 111 203 4.33e-28 115
MS.gene08539.t1 AT1G02065 63.415 82 29 1 146 226 183 264 1.22e-27 110
MS.gene08539.t1 AT3G57920 40.351 171 85 5 150 304 58 227 1.71e-27 110
MS.gene08539.t1 AT2G33810 60.811 74 29 0 151 224 54 127 9.38e-27 103
MS.gene08539.t1 AT3G60030 55.814 86 38 0 151 236 127 212 1.44e-26 111
MS.gene08539.t1 AT1G20980 53.465 101 46 1 137 236 106 206 2.06e-26 110
MS.gene08539.t1 AT2G47070 61.538 78 30 0 151 228 106 183 1.54e-25 108
MS.gene08539.t1 AT1G53160 56.962 79 34 0 151 229 54 132 5.64e-25 99.8
MS.gene08539.t1 AT1G53160 56.962 79 34 0 151 229 54 132 7.56e-25 99.4
MS.gene08539.t1 AT1G53160 56.962 79 34 0 151 229 54 132 7.56e-25 99.4
MS.gene08539.t1 AT3G15270 56.757 74 32 0 151 224 63 136 6.54e-24 97.1
MS.gene08539.t1 AT5G18830 53.333 75 35 0 150 224 137 211 1.26e-20 93.6
MS.gene08539.t1 AT5G18830 53.333 75 35 0 150 224 137 211 1.30e-20 93.6
MS.gene08539.t1 AT5G18830 53.333 75 35 0 150 224 137 211 1.34e-20 93.6
MS.gene08539.t1 AT1G02065 58.929 56 23 0 143 198 180 235 1.51e-16 78.6
MS.gene08539.t1 AT2G47070 60.377 53 21 0 176 228 2 54 1.61e-13 72.0

Find 57 sgRNAs with CRISPR-Local

Find 119 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCTTGGTGGTTGTAATTT+TGG 0.089177 4.2:-14099919 None:intergenic
CAAATAGTTCATCGTCAAAT+AGG 0.221746 4.2:+14100040 MS.gene08539:CDS
TGACTTGATTGGAGTGCTTC+AGG 0.279073 4.2:-14100701 None:intergenic
CTCCCTCTTCCCAGTGGGAC+TGG 0.294118 4.2:+14099857 MS.gene08539:CDS
CTTGCGTCATCTTTAAGTAT+TGG 0.335589 4.2:+14100222 MS.gene08539:CDS
TATAACCACCATAGGAGATT+TGG 0.339545 4.2:-14100367 None:intergenic
AGCTTTACCTGCCACATTGC+TGG 0.352012 4.2:-14100413 None:intergenic
ACAAAACTGATTGACTTGAT+TGG 0.364968 4.2:-14100712 None:intergenic
TCCCTCTTCCCAGTGGGACT+GGG 0.389604 4.2:+14099858 MS.gene08539:CDS
CCAGAGCTTTCTTCTGTCTC+TGG 0.403530 4.2:+14100108 MS.gene08539:CDS
TCTCCTATGGTGGTTATAGA+TGG 0.407218 4.2:+14100372 MS.gene08539:CDS
ACAACCCGGCATTGATTTCT+CGG 0.409997 4.2:-14099957 None:intergenic
CTTGCAATTTCCGCGCTGCC+AGG 0.416641 4.2:+14100272 MS.gene08539:CDS
AAATAGTTCATCGTCAAATA+GGG 0.430475 4.2:+14100041 MS.gene08539:CDS
CGCTGCCAGGTTGAAGGTTG+TGG 0.443635 4.2:+14100285 MS.gene08539:CDS
TTTCCGCGCTGCCAGGTTGA+AGG 0.450558 4.2:+14100279 MS.gene08539:CDS
ATTGGTGCTCCCAGTCCCAC+TGG 0.471654 4.2:-14099867 None:intergenic
CTCCCAGTCCCACTGGGAAG+AGG 0.474118 4.2:-14099860 None:intergenic
TGTTTGATGCTCCAGCTTGG+TGG 0.481479 4.2:-14099931 None:intergenic
TTTATCATTTGTATCAGAAA+CGG 0.482839 4.2:-14101907 None:intergenic
CAAAATTACAACCACCAAGC+TGG 0.484604 4.2:+14099920 MS.gene08539:CDS
TATGGTGGTTATAGATGGTA+TGG 0.485704 4.2:+14100377 MS.gene08539:CDS
CTCTTCTGTCAACTAATCCA+TGG 0.493619 4.2:+14101952 MS.gene08539:CDS
GCAGTCTCTGAATTAGCAAA+TGG 0.494415 4.2:-14101662 None:intergenic
TCTTGTTTGATGCTCCAGCT+TGG 0.510678 4.2:-14099934 None:intergenic
AAGTCATTCCAAATCTCCTA+TGG 0.520495 4.2:+14100359 MS.gene08539:CDS
CTGACTCATTGCATGAGTTA+CGG 0.521872 4.2:-14102039 None:intergenic
AACTTTCACGTCACAATGCA+AGG 0.530580 4.2:+14100663 MS.gene08539:CDS
GAACCATTGCTCACTCTACT+CGG 0.540591 4.2:+14100132 MS.gene08539:CDS
CATGATTCTGATTAAACAAG+AGG 0.549250 4.2:-14102068 None:intergenic
CAAGACCGAGAAATCAATGC+CGG 0.550620 4.2:+14099952 MS.gene08539:CDS
TCCCAGTCCCACTGGGAAGA+GGG 0.552029 4.2:-14099859 None:intergenic
TCTTCTGTCAACTAATCCAT+GGG 0.562513 4.2:+14101953 MS.gene08539:CDS
CATCGAACTCAAACAGATCG+TGG 0.568188 4.2:-14100616 None:intergenic
GATAATCTTTCGCTGATGAA+AGG 0.568732 4.2:-14100314 None:intergenic
CCAGAGACAGAAGAAAGCTC+TGG 0.576248 4.2:-14100108 None:intergenic
ATTAGAATGTTCTAGAGACA+TGG 0.583823 4.2:-14101994 None:intergenic
AGTTACGGCTTGAACAGCAC+TGG 0.590790 4.2:-14102024 None:intergenic
CAAGGCCACAACCTTCAACC+TGG 0.591428 4.2:-14100290 None:intergenic
ACGGCTTGAACAGCACTGGT+TGG 0.597812 4.2:-14102020 None:intergenic
TTAGTCGCATATGAATCCCA+TGG 0.598308 4.2:-14101969 None:intergenic
GCCGGGTTGTTCGATACACC+TGG 0.605439 4.2:+14099970 MS.gene08539:CDS
AAGACCGAGAAATCAATGCC+GGG 0.606643 4.2:+14099953 MS.gene08539:CDS
AGACTGCTACAAATTTAGCC+TGG 0.607461 4.2:+14101678 MS.gene08539:CDS
ATACCATCTATAACCACCAT+AGG 0.609763 4.2:-14100375 None:intergenic
TTGGTGCTCCCAGTCCCACT+GGG 0.623897 4.2:-14099866 None:intergenic
TTTCCGAGTAGAGTGAGCAA+TGG 0.627280 4.2:-14100135 None:intergenic
CTTTCGCTGATGAAAGGTCA+AGG 0.632145 4.2:-14100308 None:intergenic
TTAGCCTGGCAAAACATGAA+CGG 0.633733 4.2:+14101692 MS.gene08539:CDS
AATATCTCCCTCTTCCCAGT+GGG 0.634960 4.2:+14099852 MS.gene08539:CDS
CAATATCTCCCTCTTCCCAG+TGG 0.638747 4.2:+14099851 MS.gene08539:CDS
TCATTCCAAATCTCCTATGG+TGG 0.656270 4.2:+14100362 MS.gene08539:CDS
CAACCTTCAACCTGGCAGCG+CGG 0.659706 4.2:-14100282 None:intergenic
CGGAAAACGAGTGTACATTG+AGG 0.675568 4.2:+14100152 MS.gene08539:CDS
GGGTTGTTCGATACACCTGG+TGG 0.681597 4.2:+14099973 MS.gene08539:CDS
AAAGACAACACTAACACACA+AGG 0.691256 4.2:+14101734 MS.gene08539:CDS
ACCAGGTGTATCGAACAACC+CGG 0.701562 4.2:-14099971 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATTTTTTTTTTTTTTTT+TGG + chr4.2:14100479-14100498 MS.gene08539:intron 0.0%
!! AAATAATTATCATGATTGTT+TGG + chr4.2:14101347-14101366 MS.gene08539:intron 15.0%
!!! ATTCACTTTTAGTTTTTTAT+AGG + chr4.2:14101508-14101527 MS.gene08539:intron 15.0%
!!! GTTTATTGTAGTTTTTTTAT+AGG + chr4.2:14101530-14101549 MS.gene08539:intron 15.0%
!!! TTCTAACTTCTAATATATTT+TGG + chr4.2:14101611-14101630 MS.gene08539:intron 15.0%
!! ATAATTACTTATTGTCAGAA+AGG + chr4.2:14101210-14101229 MS.gene08539:intron 20.0%
!! TTTATCATTTGTATCAGAAA+CGG - chr4.2:14101910-14101929 None:intergenic 20.0%
!!! GTTGTTTTTTTTACAAAATC+AGG - chr4.2:14101449-14101468 None:intergenic 20.0%
!!! TTTAGTTTCATAGATCTTAT+TGG + chr4.2:14101054-14101073 MS.gene08539:intron 20.0%
! AAATAGTTCATCGTCAAATA+GGG + chr4.2:14100041-14100060 MS.gene08539:CDS 25.0%
! AACTAAAAGTGAATGTTCTT+TGG - chr4.2:14101502-14101521 None:intergenic 25.0%
! TGTTTCAATGTAATTGACAT+AGG + chr4.2:14100454-14100473 MS.gene08539:intron 25.0%
! TTGTATCATATGCCTTAATA+AGG - chr4.2:14101329-14101348 None:intergenic 25.0%
!! ATTGCTATATTTAGAGTTGT+GGG + chr4.2:14101235-14101254 MS.gene08539:intron 25.0%
!! TATTGCTATATTTAGAGTTG+TGG + chr4.2:14101234-14101253 MS.gene08539:intron 25.0%
!!! AAAACATGTATTTTTGCTTG+TGG + chr4.2:14100072-14100091 MS.gene08539:CDS 25.0%
!!! AGGTTTTTGGAATTACTTTT+CGG - chr4.2:14100186-14100205 None:intergenic 25.0%
!!! CAAAAACCTATCTTTTTCAA+GGG + chr4.2:14100197-14100216 MS.gene08539:CDS 25.0%
!!! CAAAATCAGGTTTTAAAGAA+TGG - chr4.2:14101436-14101455 None:intergenic 25.0%
AAACGTGAAATAACCTCAAT+TGG - chr4.2:14101829-14101848 None:intergenic 30.0%
AAAGCTGAAAAGATGAAATG+TGG + chr4.2:14100815-14100834 MS.gene08539:intron 30.0%
ACAAATCAGCACAAAAAATG+TGG - chr4.2:14101856-14101875 None:intergenic 30.0%
ATGAGCAGAACTAAAAAGAT+AGG - chr4.2:14101028-14101047 None:intergenic 30.0%
CAAATAGTTCATCGTCAAAT+AGG + chr4.2:14100040-14100059 MS.gene08539:CDS 30.0%
CAAGAACTTTGGATGTAAAA+TGG - chr4.2:14101793-14101812 None:intergenic 30.0%
GCAATGTTTATTGTCAACTA+AGG - chr4.2:14100865-14100884 None:intergenic 30.0%
TTTGGATGTAAAATGGACAA+CGG - chr4.2:14101786-14101805 None:intergenic 30.0%
! CCTAAGTCTTTAGAGAATTA+AGG + chr4.2:14101294-14101313 MS.gene08539:intron 30.0%
! CCTTAATTCTCTAAAGACTT+AGG - chr4.2:14101297-14101316 None:intergenic 30.0%
! CCTTGAAAAAGATAGGTTTT+TGG - chr4.2:14100199-14100218 None:intergenic 30.0%
! TAACCTCAATTGGATACTTT+TGG - chr4.2:14101819-14101838 None:intergenic 30.0%
! TGTTAGTGTTGTCTTTTGTT+TGG - chr4.2:14101730-14101749 None:intergenic 30.0%
!! ACAAAACTGATTGACTTGAT+TGG - chr4.2:14100715-14100734 None:intergenic 30.0%
!! ATTAGAATGTTCTAGAGACA+TGG - chr4.2:14101997-14102016 None:intergenic 30.0%
!! CCAAAAACCTATCTTTTTCA+AGG + chr4.2:14100196-14100215 MS.gene08539:CDS 30.0%
!! CTGATTTGTTATAACTTTGC+AGG + chr4.2:14101867-14101886 MS.gene08539:intron 30.0%
!! TCTGCTTGTTATCTATGTTT+GGG + chr4.2:14100788-14100807 MS.gene08539:intron 30.0%
!! TTCTGCTTGTTATCTATGTT+TGG + chr4.2:14100787-14100806 MS.gene08539:intron 30.0%
!!! AACTTGCTGTCTTTTTTGAA+TGG + chr4.2:14100561-14100580 MS.gene08539:intron 30.0%
AAAATGGACAACGGAATCAT+CGG - chr4.2:14101777-14101796 None:intergenic 35.0%
AAACTCTTACGCAAATAAGC+AGG - chr4.2:14100942-14100961 None:intergenic 35.0%
AAAGACAACACTAACACACA+AGG + chr4.2:14101734-14101753 MS.gene08539:CDS 35.0%
AAGTCATTCCAAATCTCCTA+TGG + chr4.2:14100359-14100378 MS.gene08539:CDS 35.0%
ATACCATCTATAACCACCAT+AGG - chr4.2:14100378-14100397 None:intergenic 35.0%
CAACTAAGGTATGCATAACA+TGG - chr4.2:14100851-14100870 None:intergenic 35.0%
CAAGATCCCTTGAAAAAGAT+AGG - chr4.2:14100206-14100225 None:intergenic 35.0%
CTTGCGTCATCTTTAAGTAT+TGG + chr4.2:14100222-14100241 MS.gene08539:CDS 35.0%
GATAATCTTTCGCTGATGAA+AGG - chr4.2:14100317-14100336 None:intergenic 35.0%
GTTAGTAAAGACTTCAGCTT+AGG - chr4.2:14101395-14101414 None:intergenic 35.0%
GTTTACCTTGCAAGTTACAA+TGG + chr4.2:14100958-14100977 MS.gene08539:intron 35.0%
TATAACCACCATAGGAGATT+TGG - chr4.2:14100370-14100389 None:intergenic 35.0%
TATGGTGGTTATAGATGGTA+TGG + chr4.2:14100377-14100396 MS.gene08539:CDS 35.0%
TCTTCTGTCAACTAATCCAT+GGG + chr4.2:14101953-14101972 MS.gene08539:CDS 35.0%
TGAGGACTGGATATAGTTAA+AGG + chr4.2:14101259-14101278 MS.gene08539:intron 35.0%
TTATTTGCTTCGAATGCTGA+AGG + chr4.2:14100591-14100610 MS.gene08539:intron 35.0%
! AATGTCCATTGTAACTTGCA+AGG - chr4.2:14100966-14100985 None:intergenic 35.0%
! ATATTTAGAGTTGTGGGATG+AGG + chr4.2:14101241-14101260 MS.gene08539:intron 35.0%
! CTTTTGTGTGGAAGAATGAT+TGG - chr4.2:14099888-14099907 None:intergenic 35.0%
!! AACATGACTGTTACTTTGCA+TGG - chr4.2:14101183-14101202 None:intergenic 35.0%
!! ACTTTTGGTTGCAAGAACTT+TGG - chr4.2:14101804-14101823 None:intergenic 35.0%
!! CAACCAAAAGTATCCAATTG+AGG + chr4.2:14101813-14101832 MS.gene08539:intron 35.0%
!!! ATATATTTTGGTTCCGCAGA+TGG + chr4.2:14101623-14101642 MS.gene08539:intron 35.0%
!!! ATATTTTTGGCTCTGCATCA+AGG + chr4.2:14100001-14100020 MS.gene08539:CDS 35.0%
!!! GATTTTCAGTTGCTTTTGTG+TGG - chr4.2:14099900-14099919 None:intergenic 35.0%
AACTTTCACGTCACAATGCA+AGG + chr4.2:14100663-14100682 MS.gene08539:CDS 40.0%
AGACTGCTACAAATTTAGCC+TGG + chr4.2:14101678-14101697 MS.gene08539:CDS 40.0%
AGGTAGCGTACTCCTTATTA+AGG + chr4.2:14101314-14101333 MS.gene08539:intron 40.0%
CAAAATTACAACCACCAAGC+TGG + chr4.2:14099920-14099939 MS.gene08539:CDS 40.0%
CATACGTTTCGATGGTAAAG+AGG + chr4.2:14101089-14101108 MS.gene08539:intron 40.0%
CCAAAAATATCACTTCCACC+AGG - chr4.2:14099991-14100010 None:intergenic 40.0%
CTCTTCTGTCAACTAATCCA+TGG + chr4.2:14101952-14101971 MS.gene08539:CDS 40.0%
CTGACTCATTGCATGAGTTA+CGG - chr4.2:14102042-14102061 None:intergenic 40.0%
GCAGTCTCTGAATTAGCAAA+TGG - chr4.2:14101665-14101684 None:intergenic 40.0%
TCAACTGTCATACGTTTCGA+TGG + chr4.2:14101081-14101100 MS.gene08539:intron 40.0%
TCATTCCAAATCTCCTATGG+TGG + chr4.2:14100362-14100381 MS.gene08539:CDS 40.0%
TCTCCTATGGTGGTTATAGA+TGG + chr4.2:14100372-14100391 MS.gene08539:CDS 40.0%
TTAGCCTGGCAAAACATGAA+CGG + chr4.2:14101692-14101711 MS.gene08539:CDS 40.0%
TTAGTCGCATATGAATCCCA+TGG - chr4.2:14101972-14101991 None:intergenic 40.0%
! CCTGGTGGAAGTGATATTTT+TGG + chr4.2:14099988-14100007 MS.gene08539:CDS 40.0%
!! CAGCTTGGTGGTTGTAATTT+TGG - chr4.2:14099922-14099941 None:intergenic 40.0%
!! CTTTTGTTTGGAAGAGCTTG+CGG - chr4.2:14101718-14101737 None:intergenic 40.0%
AAGACCGAGAAATCAATGCC+GGG + chr4.2:14099953-14099972 MS.gene08539:CDS 45.0%
AATATCTCCCTCTTCCCAGT+GGG + chr4.2:14099852-14099871 MS.gene08539:CDS 45.0%
CAAGACCGAGAAATCAATGC+CGG + chr4.2:14099952-14099971 MS.gene08539:CDS 45.0%
CATCGAACTCAAACAGATCG+TGG - chr4.2:14100619-14100638 None:intergenic 45.0%
CGGAAAACGAGTGTACATTG+AGG + chr4.2:14100152-14100171 MS.gene08539:CDS 45.0%
CTTTCGCTGATGAAAGGTCA+AGG - chr4.2:14100311-14100330 None:intergenic 45.0%
GAACCATTGCTCACTCTACT+CGG + chr4.2:14100132-14100151 MS.gene08539:CDS 45.0%
TTTCCGAGTAGAGTGAGCAA+TGG - chr4.2:14100138-14100157 None:intergenic 45.0%
! TCTTGTTTGATGCTCCAGCT+TGG - chr4.2:14099937-14099956 None:intergenic 45.0%
! TGACTTGATTGGAGTGCTTC+AGG - chr4.2:14100704-14100723 None:intergenic 45.0%
!! ACAACCCGGCATTGATTTCT+CGG - chr4.2:14099960-14099979 None:intergenic 45.0%
!! CATCTGTTGCTTTCCATCTG+CGG - chr4.2:14101639-14101658 None:intergenic 45.0%
!! TCAGTTTTGTCTTCACGCTG+TGG + chr4.2:14100725-14100744 MS.gene08539:CDS 45.0%
ACCAGGTGTATCGAACAACC+CGG - chr4.2:14099974-14099993 None:intergenic 50.0%
AGCTTTACCTGCCACATTGC+TGG - chr4.2:14100416-14100435 None:intergenic 50.0%
AGTTACGGCTTGAACAGCAC+TGG - chr4.2:14102027-14102046 None:intergenic 50.0%
CAATATCTCCCTCTTCCCAG+TGG + chr4.2:14099851-14099870 MS.gene08539:CDS 50.0%
CCAGAGACAGAAGAAAGCTC+TGG - chr4.2:14100111-14100130 None:intergenic 50.0%
CCAGAGCTTTCTTCTGTCTC+TGG + chr4.2:14100108-14100127 MS.gene08539:CDS 50.0%
TAGAGTTGTGGGATGAGGAC+TGG + chr4.2:14101246-14101265 MS.gene08539:intron 50.0%
TGTTTGATGCTCCAGCTTGG+TGG - chr4.2:14099934-14099953 None:intergenic 50.0%
! GATTTTGCCAGCAATGTGGC+AGG + chr4.2:14100406-14100425 MS.gene08539:CDS 50.0%
!!! CGTCGATTTTGCCAGCAATG+TGG + chr4.2:14100402-14100421 MS.gene08539:CDS 50.0%
CAAGGCCACAACCTTCAACC+TGG - chr4.2:14100293-14100312 None:intergenic 55.0%
GGGTTGTTCGATACACCTGG+TGG + chr4.2:14099973-14099992 MS.gene08539:CDS 55.0%
!! ACGGCTTGAACAGCACTGGT+TGG - chr4.2:14102023-14102042 None:intergenic 55.0%
CAACCTTCAACCTGGCAGCG+CGG - chr4.2:14100285-14100304 None:intergenic 60.0%
CTTGCAATTTCCGCGCTGCC+AGG + chr4.2:14100272-14100291 MS.gene08539:CDS 60.0%
GCCGGGTTGTTCGATACACC+TGG + chr4.2:14099970-14099989 MS.gene08539:CDS 60.0%
TCCCAGTCCCACTGGGAAGA+GGG - chr4.2:14099862-14099881 None:intergenic 60.0%
TCCCTCTTCCCAGTGGGACT+GGG + chr4.2:14099858-14099877 MS.gene08539:CDS 60.0%
! ATTGGTGCTCCCAGTCCCAC+TGG - chr4.2:14099870-14099889 None:intergenic 60.0%
! CGCTGCCAGGTTGAAGGTTG+TGG + chr4.2:14100285-14100304 MS.gene08539:CDS 60.0%
! GCGGCCGTTCATGTTTTGCC+AGG - chr4.2:14101699-14101718 None:intergenic 60.0%
! TTGGTGCTCCCAGTCCCACT+GGG - chr4.2:14099869-14099888 None:intergenic 60.0%
! TTTCCGCGCTGCCAGGTTGA+AGG + chr4.2:14100279-14100298 MS.gene08539:CDS 60.0%
CTCCCAGTCCCACTGGGAAG+AGG - chr4.2:14099863-14099882 None:intergenic 65.0%
CTCCCTCTTCCCAGTGGGAC+TGG + chr4.2:14099857-14099876 MS.gene08539:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 14099838 14102079 14099838 ID=MS.gene08539
chr4.2 mRNA 14099838 14102079 14099838 ID=MS.gene08539.t1;Parent=MS.gene08539
chr4.2 exon 14099838 14100427 14099838 ID=MS.gene08539.t1.exon1;Parent=MS.gene08539.t1
chr4.2 CDS 14099838 14100427 14099838 ID=cds.MS.gene08539.t1;Parent=MS.gene08539.t1
chr4.2 exon 14100613 14100746 14100613 ID=MS.gene08539.t1.exon2;Parent=MS.gene08539.t1
chr4.2 CDS 14100613 14100746 14100613 ID=cds.MS.gene08539.t1;Parent=MS.gene08539.t1
chr4.2 exon 14101642 14101755 14101642 ID=MS.gene08539.t1.exon3;Parent=MS.gene08539.t1
chr4.2 CDS 14101642 14101755 14101642 ID=cds.MS.gene08539.t1;Parent=MS.gene08539.t1
chr4.2 exon 14101889 14102079 14101889 ID=MS.gene08539.t1.exon4;Parent=MS.gene08539.t1
chr4.2 CDS 14101889 14102079 14101889 ID=cds.MS.gene08539.t1;Parent=MS.gene08539.t1
Gene Sequence

>MS.gene08539

ATGGACTTGAATGCAATATCTCCCTCTTCCCAGTGGGACTGGGAGCACCAATCATTCTTCCACACAAAAGCAACTGAAAATCCAAAATTACAACCACCAAGCTGGAGCATCAAACAAGACCGAGAAATCAATGCCGGGTTGTTCGATACACCTGGTGGAAGTGATATTTTTGGCTCTGCATCAAGGAGTTCAAAATCAGCTTCAAATAGTTCATCGTCAAATAGGGATAATAGCAAAACATGTATTTTTGCTTGTGGAAATTCTCATACACCAGAGCTTTCTTCTGTCTCTGGTGAACCATTGCTCACTCTACTCGGAAAACGAGTGTACATTGAGGATGTATGTCCGAAAAGTAATTCCAAAAACCTATCTTTTTCAAGGGATCTTGCGTCATCTTTAAGTATTGGAAAGAAATGTAAGTTCAATAGTCAGAACTTGCAATTTCCGCGCTGCCAGGTTGAAGGTTGTGGCCTTGACCTTTCATCAGCGAAAGATTATCATCGCAAACGAAGAGTTTGTGAAAGTCATTCCAAATCTCCTATGGTGGTTATAGATGGTATGGAACGTCGATTTTGCCAGCAATGTGGCAGGTAAAGCTTCTTAACAAATGTATGATTGTTTCAATGTAATTGACATAGGAAATAATTTTTTTTTTTTTTTTTGGTAGTGACGAAATGTTGTTAGCACATCATTATAAGCTAATATCACTACTCAGTCTGATTAAACTTGCTGTCTTTTTTGAATGGTGTTGAATTATTTGCTTCGAATGCTGAAGGTTCCACGATCTGTTTGAGTTCGATGAAAAGAAAAGAAGCTGCAGAGAACAACTTTCACGTCACAATGCAAGGCGACGTAAGCATCAGCCTGAAGCACTCCAATCAAGTCAATCAGTTTTGTCTTCACGCTGTGGTAATACTTATTTTTCAATAGTTTTGCAGTTTATCTCAGTTTCTGCTTGTTATCTATGTTTGGGTGTTAAAGCTGAAAAGATGAAATGTGGAAACACAAGACCATGTTATGCATACCTTAGTTGACAATAAACATTGCTGAATGAAAGATGTTTTAAAACTTGACATGCTTAACGTCACATTTCTTTGCTGTCCTGCTTATTTGCGTAAGAGTTTACCTTGCAAGTTACAATGGACATTAAATGTGATGTGACAGAACTTCTTAATTTGCTACAATTTCCTATCTTTTTAGTTCTGCTCATTTTTTCTTTAGTTTCATAGATCTTATTGGATTGTCAACTGTCATACGTTTCGATGGTAAAGAGGCTTAATCTTCTGTAACATGAATAAGTTAAGCATGTTCAAGAGTTCTAGCTGTAACAATTTTATAATAACCATGCAAAGTAACAGTCATGTTATTCTTGATAATTACTTATTGTCAGAAAGGATATTGCTATATTTAGAGTTGTGGGATGAGGACTGGATATAGTTAAAGGCAGATTTAAGAGCCTAAGTCTTTAGAGAATTAAGGTAGCGTACTCCTTATTAAGGCATATGATACAAATAATTATCATGATTGTTTGGACTTGAGTGTGATTATGACATGCCTAAGCTGAAGTCTTTACTAACTAGATTTGTATGAGTATGCCATTCTTTAAAACCTGATTTTGTAAAAAAAACAACTTTAAATTTTGCTGTTAGTTGAAACATAGACCAAAGAACATTCACTTTTAGTTTTTTATAGGTTTATTGTAGTTTTTTTATAGGTTTATTGTAGTTAATTGCAAGTCGTTTATTTCTCAATGATTCAAATTTTACTGCTCTCTTCTAACTTCTAATATATTTTGGTTCCGCAGATGGAAAGCAACAGATGAGTCCATTTGCTAATTCAGAGACTGCTACAAATTTAGCCTGGCAAAACATGAACGGCCGCAAGCTCTTCCAAACAAAAGACAACACTAACACACAAGGTACTGTCACTATGCTTTCCGATGATTCCGTTGTCCATTTTACATCCAAAGTTCTTGCAACCAAAAGTATCCAATTGAGGTTATTTCACGTTTATTGCCACATTTTTTGTGCTGATTTGTTATAACTTTGCAGGTGTAGAAGATTTCATTACCGTTTCTGATACAAATGATAAACAAGACTTTACTTGTGCTCTCTCTCTTCTGTCAACTAATCCATGGGATTCATATGCGACTAAATCCATGTCTCTAGAACATTCTAATCGTCCAACCAGTGCTGTTCAAGCCGTAACTCATGCAATGAGTCAGCGCGTGCCTCTTGTTTAA
Protein sequence

>MS.gene08539.t1

MDLNAISPSSQWDWEHQSFFHTKATENPKLQPPSWSIKQDREINAGLFDTPGGSDIFGSASRSSKSASNSSSSNRDNSKTCIFACGNSHTPELSSVSGEPLLTLLGKRVYIEDVCPKSNSKNLSFSRDLASSLSIGKKCKFNSQNLQFPRCQVEGCGLDLSSAKDYHRKRRVCESHSKSPMVVIDGMERRFCQQCGRFHDLFEFDEKKRSCREQLSRHNARRRKHQPEALQSSQSVLSSRCDGKQQMSPFANSETATNLAWQNMNGRKLFQTKDNTNTQGVEDFITVSDTNDKQDFTCALSLLSTNPWDSYATKSMSLEHSNRPTSAVQAVTHAMSQRVPLV