AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene08541

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08541.t1 MTR_8g080670 91.220 410 36 0 1 410 1 410 0.0 710
MS.gene08541.t1 MTR_8g080680 61.187 438 106 3 1 422 1 390 2.91e-178 503
MS.gene08541.t1 MTR_8g080690 61.139 404 113 6 1 388 1 376 4.92e-141 408
MS.gene08541.t1 MTR_3g085180 50.110 453 189 12 1 435 1 434 1.65e-113 340
MS.gene08541.t1 MTR_1g053715 35.517 290 146 8 123 386 17 291 2.65e-35 134
MS.gene08541.t1 MTR_8g463140 63.158 76 28 0 159 234 62 137 4.88e-29 112
MS.gene08541.t1 MTR_7g092930 65.789 76 26 0 159 234 65 140 2.45e-28 114
MS.gene08541.t1 MTR_4g088555 58.140 86 34 1 150 235 53 136 3.79e-27 106
MS.gene08541.t1 MTR_2g078770 54.945 91 40 1 144 234 41 130 4.49e-27 106
MS.gene08541.t1 MTR_8g005960 44.521 146 70 4 94 234 120 259 5.56e-27 110
MS.gene08541.t1 MTR_7g444860 45.038 131 52 2 116 234 99 221 1.12e-25 106
MS.gene08541.t1 MTR_5g046670 58.667 75 31 0 160 234 242 316 1.61e-24 106
MS.gene08541.t1 MTR_4g109770 39.490 157 71 5 99 234 42 195 3.51e-24 104
MS.gene08541.t1 MTR_2g014200 57.333 75 32 0 160 234 62 136 7.95e-24 97.1
MS.gene08541.t1 MTR_7g028740 51.765 85 41 0 150 234 65 149 3.84e-23 100
MS.gene08541.t1 MTR_3g099080 45.872 109 57 1 162 270 98 204 8.78e-23 99.8
MS.gene08541.t1 MTR_2g461920 41.406 128 59 3 122 234 117 243 1.38e-22 100
MS.gene08541.t1 MTR_1g035010 45.872 109 51 2 127 234 75 176 2.16e-21 97.8
MS.gene08541.t1 MTR_2g046550 46.218 119 56 2 127 241 104 218 2.81e-21 97.4
MS.gene08541.t1 MTR_8g096780 44.737 114 60 1 162 275 99 209 3.02e-21 95.5
MS.gene08541.t1 MTR_1g086250 56.757 74 32 0 161 234 146 219 1.24e-20 95.5
MS.gene08541.t1 MTR_7g110320 38.125 160 87 4 127 286 113 260 2.78e-20 94.4
MS.gene08541.t1 MTR_7g110320 38.125 160 87 4 127 286 113 260 3.65e-20 94.0
MS.gene08541.t1 MTR_2g020620 32.934 167 95 5 169 329 97 252 1.00e-15 80.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08541.t1 AT1G27360 42.353 255 118 8 1 234 1 247 1.12e-37 141
MS.gene08541.t1 AT1G27360 42.353 255 118 8 1 234 1 247 1.12e-37 141
MS.gene08541.t1 AT1G27360 42.353 255 118 8 1 234 1 247 1.12e-37 141
MS.gene08541.t1 AT1G27360 42.353 255 118 8 1 234 1 247 1.12e-37 141
MS.gene08541.t1 AT5G43270 34.253 435 195 21 1 393 1 386 1.81e-37 141
MS.gene08541.t1 AT5G43270 34.253 435 195 21 1 393 1 386 1.81e-37 141
MS.gene08541.t1 AT5G43270 34.253 435 195 21 1 393 1 386 1.81e-37 141
MS.gene08541.t1 AT1G27370 41.633 245 114 7 12 234 11 248 6.96e-37 138
MS.gene08541.t1 AT1G27370 41.633 245 114 7 12 234 11 248 2.49e-36 138
MS.gene08541.t1 AT1G27370 41.633 245 114 7 12 234 11 248 2.49e-36 138
MS.gene08541.t1 AT1G27370 41.633 245 114 7 12 234 11 248 2.49e-36 138
MS.gene08541.t1 AT1G27370 41.633 245 114 7 12 234 11 248 2.49e-36 138
MS.gene08541.t1 AT1G27370 41.633 245 114 7 12 234 11 248 2.49e-36 138
MS.gene08541.t1 AT1G27370 41.633 245 114 7 12 234 11 248 2.49e-36 138
MS.gene08541.t1 AT2G42200 45.517 145 56 4 160 304 72 193 1.25e-28 116
MS.gene08541.t1 AT3G57920 49.541 109 48 2 129 234 27 131 1.95e-27 112
MS.gene08541.t1 AT1G02065 50.000 114 51 1 127 234 147 260 4.13e-27 111
MS.gene08541.t1 AT1G69170 62.069 87 30 2 150 234 111 196 6.95e-27 112
MS.gene08541.t1 AT2G33810 58.667 75 31 0 161 235 53 127 8.39e-25 99.4
MS.gene08541.t1 AT3G15270 56.757 74 32 0 161 234 62 135 3.03e-23 96.7
MS.gene08541.t1 AT1G53160 58.108 74 31 0 161 234 53 126 2.38e-22 94.0
MS.gene08541.t1 AT1G53160 58.108 74 31 0 161 234 53 126 2.38e-22 94.0
MS.gene08541.t1 AT1G53160 58.108 74 31 0 161 234 53 126 2.72e-22 93.6
MS.gene08541.t1 AT5G50670 54.667 75 34 0 160 234 99 173 3.68e-22 97.8
MS.gene08541.t1 AT5G50670 54.667 75 34 0 160 234 99 173 3.68e-22 97.8
MS.gene08541.t1 AT5G50570 54.667 75 34 0 160 234 99 173 3.68e-22 97.8
MS.gene08541.t1 AT5G50570 54.667 75 34 0 160 234 99 173 3.68e-22 97.8
MS.gene08541.t1 AT3G60030 57.534 73 31 0 162 234 127 199 2.73e-21 97.4
MS.gene08541.t1 AT2G47070 48.958 96 42 1 139 234 90 178 1.02e-20 95.5
MS.gene08541.t1 AT1G20980 56.000 75 33 0 160 234 118 192 4.17e-20 94.0
MS.gene08541.t1 AT1G76580 56.000 75 33 0 160 234 112 186 1.85e-19 91.7
MS.gene08541.t1 AT1G76580 56.000 75 33 0 160 234 112 186 2.05e-19 91.3
MS.gene08541.t1 AT1G76580 56.000 75 33 0 160 234 112 186 3.50e-19 90.9
MS.gene08541.t1 AT1G02065 47.191 89 41 1 127 209 147 235 2.16e-17 81.6
MS.gene08541.t1 AT5G18830 46.575 73 39 0 162 234 138 210 6.26e-16 80.9
MS.gene08541.t1 AT5G18830 46.575 73 39 0 162 234 138 210 6.30e-16 80.9
MS.gene08541.t1 AT5G18830 46.575 73 39 0 162 234 138 210 6.76e-16 80.5
MS.gene08541.t1 AT2G47070 60.417 48 19 0 187 234 2 49 1.73e-11 66.6

Find 78 sgRNAs with CRISPR-Local

Find 152 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATGATCTGTTTGAGTTTGA+TGG 0.225842 4.2:+14110167 MS.gene08541:CDS
CATTGATCCTCTTGGTTTCA+AGG 0.239743 4.2:-14111280 None:intergenic
CAAGGACTTTGGATGTAAAA+TGG 0.260990 4.2:-14111262 None:intergenic
TCAATCTAAAACTCGGTAAA+AGG 0.273664 4.2:+14109720 MS.gene08541:CDS
ACTTTCAAATGCAAATTTAC+TGG 0.299044 4.2:-14109597 None:intergenic
TATAACTACCATAGGAGATT+TGG 0.321384 4.2:-14109903 None:intergenic
GCTTCAATAGGCCCTTCTTT+AGG 0.325945 4.2:-14109653 None:intergenic
CCTCTTGGTTTCAAGGACTT+TGG 0.327846 4.2:-14111273 None:intergenic
AGCTTTACCTGCTACATTGC+TGG 0.356873 4.2:-14109949 None:intergenic
ACAAAGATGATTGACTTGAT+TGG 0.359832 4.2:-14110262 None:intergenic
CTCTAGCCTCATCAGAATAT+TGG 0.364549 4.2:+14111704 MS.gene08541:CDS
GTGCCAATGTTGTCGTTTAT+AGG 0.365991 4.2:-14111200 None:intergenic
TATGGTAGTTATAGATGGTT+TGG 0.373519 4.2:+14109913 MS.gene08541:CDS
CTCCCTCTTCACGGTGGGAA+TGG 0.375697 4.2:+14109360 MS.gene08541:CDS
GACATTCATGTTAGTCCAAC+TGG 0.375986 4.2:-14111843 None:intergenic
GCAGTCTTTGAATAAGCAAA+TGG 0.377299 4.2:-14111110 None:intergenic
CAAAGATGATTGACTTGATT+GGG 0.382950 4.2:-14110261 None:intergenic
CCGCCTCTCTGCCAGGTTGA+AGG 0.385535 4.2:+14109815 MS.gene08541:CDS
TCTCCTATGGTAGTTATAGA+TGG 0.387501 4.2:+14109908 MS.gene08541:CDS
CTGACCTGCATTGATCCTCT+TGG 0.395116 4.2:-14111288 None:intergenic
CTCTGCCAGGTTGAAGGTTG+TGG 0.395224 4.2:+14109821 MS.gene08541:CDS
TGCTATGGCTTGAGCGGTAC+TGG 0.416456 4.2:-14111659 None:intergenic
CCAACTGGAAGTGGTTATTC+TGG 0.417567 4.2:+14109491 MS.gene08541:CDS
TCCCTCTTCACGGTGGGAAT+GGG 0.429201 4.2:+14109361 MS.gene08541:CDS
CATCAAACTCAAACAGATCA+TGG 0.444519 4.2:-14110166 None:intergenic
GGTGCATTAGAAGCTTCAAT+AGG 0.450824 4.2:-14109665 None:intergenic
ATGTTCTAGAGAAACGGATT+TGG 0.456583 4.2:-14111623 None:intergenic
AAAATTGAATTACCTAAAGA+AGG 0.460103 4.2:+14109641 MS.gene08541:CDS
ATCTAAATGTTCTAGAGAAA+CGG 0.467117 4.2:-14111629 None:intergenic
TGGTTGTTCGATACGCCAAC+TGG 0.470221 4.2:+14109476 MS.gene08541:CDS
CAATCAGTTCATCACCAACT+AGG 0.472722 4.2:+14109561 MS.gene08541:CDS
TTCGATACGCCAACTGGAAG+TGG 0.476010 4.2:+14109482 MS.gene08541:CDS
ACATTGTAAGTTCTGAGCAT+TGG 0.495722 4.2:-14109792 None:intergenic
AATTCGATACTCCAGTTTGG+TGG 0.496264 4.2:-14109434 None:intergenic
GACGCGTCATCTTTAGTTGT+TGG 0.503063 4.2:+14109755 MS.gene08541:CDS
ACAATGTCCGCCTCTCTGCC+AGG 0.504313 4.2:+14109808 MS.gene08541:CDS
ATGCAAAATCTCCCTCTTCA+CGG 0.507595 4.2:+14109351 MS.gene08541:CDS
TCTAATTCGATACTCCAGTT+TGG 0.509220 4.2:-14109437 None:intergenic
TGACAGAAGAGAGGAAGCAC+AGG 0.512878 4.2:-14111575 None:intergenic
CAGTCTTTGAATAAGCAAAT+GGG 0.517662 4.2:-14111109 None:intergenic
GATAGCATAGTTACAGTACT+TGG 0.519974 4.2:-14111224 None:intergenic
CTCTTCTGTCAACCAGTCCG+TGG 0.520942 4.2:+14111587 MS.gene08541:CDS
TTTACCATTGGAATCATCAT+GGG 0.526394 4.2:-14109621 None:intergenic
CGTCATCTTTAGTTGTTGGA+AGG 0.527554 4.2:+14109759 MS.gene08541:CDS
AGATCATTCCAAATCTCCTA+TGG 0.535644 4.2:+14109895 MS.gene08541:CDS
TTGTGGCCTTGACCTTTCAT+CGG 0.541799 4.2:+14109838 MS.gene08541:CDS
CGCATATGAATCCCACGGAC+TGG 0.545835 4.2:-14111599 None:intergenic
TAGACCGAGAAAATAATGTC+TGG 0.549417 4.2:+14109456 MS.gene08541:CDS
AATCAGTTCATCACCAACTA+GGG 0.553568 4.2:+14109562 MS.gene08541:CDS
TCCCATTCCCACCGTGAAGA+GGG 0.557718 4.2:-14109362 None:intergenic
AAACCATCTATAACTACCAT+AGG 0.563385 4.2:-14109911 None:intergenic
CTGACTCGTTGTGTGTGCTA+TGG 0.564583 4.2:-14111674 None:intergenic
ACTGGTGAAAACACGACTCA+AGG 0.568330 4.2:-14111825 None:intergenic
TGAGTTTGAGATCCACATGC+TGG 0.569545 4.2:-14111755 None:intergenic
ATCCCATTCCCACCGTGAAG+AGG 0.571348 4.2:-14109363 None:intergenic
TCTTCTGTCAACCAGTCCGT+GGG 0.576912 4.2:+14111588 MS.gene08541:CDS
AAATTGAATTACCTAAAGAA+GGG 0.590524 4.2:+14109642 MS.gene08541:CDS
CAAGGCCACAACCTTCAACC+TGG 0.591428 4.2:-14109826 None:intergenic
AAAATCTCCCTCTTCACGGT+GGG 0.595195 4.2:+14109355 MS.gene08541:CDS
CGGTAAAAGGATGAAATTCG+AGG 0.600138 4.2:+14109733 MS.gene08541:CDS
CTAGATACACAAGAAAGCAT+TGG 0.604314 4.2:-14109686 None:intergenic
CCATTGCTCAATCTAAAACT+CGG 0.604403 4.2:+14109713 MS.gene08541:CDS
AAGCCTATAAACGACAACAT+TGG 0.614145 4.2:+14111197 MS.gene08541:CDS
GAAACCAAGAGGATCAATGC+AGG 0.614595 4.2:+14111284 MS.gene08541:CDS
AGTTCCCATGATGATTCCAA+TGG 0.616986 4.2:+14109617 MS.gene08541:CDS
CCAGAATAACCACTTCCAGT+TGG 0.626070 4.2:-14109491 None:intergenic
CAATATTCTGATGAGGCTAG+AGG 0.629859 4.2:-14111703 None:intergenic
AATATGTCGATTCCAGCATG+TGG 0.630965 4.2:+14111743 MS.gene08541:CDS
CAACCTTCAACCTGGCAGAG+AGG 0.635133 4.2:-14109818 None:intergenic
CCAAAGTCCTTGAAACCAAG+AGG 0.636381 4.2:+14111273 MS.gene08541:CDS
TTTGGATGTAAAATGGACAT+CGG 0.653646 4.2:-14111255 None:intergenic
CGGACTGGTTGACAGAAGAG+AGG 0.658311 4.2:-14111584 None:intergenic
ACTGTGCCAATATTCTGATG+AGG 0.658353 4.2:-14111710 None:intergenic
CAAAATCTCCCTCTTCACGG+TGG 0.658631 4.2:+14109354 MS.gene08541:CDS
CCTTCAACCTGGCAGAGAGG+CGG 0.660903 4.2:-14109815 None:intergenic
TCTTTGTCTTCGTCACCCTG+TGG 0.667989 4.2:+14110278 MS.gene08541:CDS
TTGGTCGCATATGAATCCCA+CGG 0.682290 4.2:-14111604 None:intergenic
TGTGTGTGCTATGGCTTGAG+CGG 0.704952 4.2:-14111665 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAACATAATCTATTTATTT+TGG + chr4.2:14111378-14111397 MS.gene08541:intron 10.0%
!! AAACATAAATGAACTAAAAT+TGG - chr4.2:14110981-14111000 None:intergenic 15.0%
!!! AGGAAATAATAGTATTATTT+TGG + chr4.2:14110010-14110029 MS.gene08541:intron 15.0%
!!! ATTAAACTTGCTTTTTTTTT+TGG + chr4.2:14110087-14110106 MS.gene08541:intron 15.0%
!! AAAATTGAATTACCTAAAGA+AGG + chr4.2:14109641-14109660 MS.gene08541:CDS 20.0%
!! AAATTGAATTACCTAAAGAA+GGG + chr4.2:14109642-14109661 MS.gene08541:CDS 20.0%
!! TGTAATTGATTAGAGTAATA+TGG + chr4.2:14111438-14111457 MS.gene08541:intron 20.0%
!!! AGTTGAATTTTTATTACATG+AGG - chr4.2:14110923-14110942 None:intergenic 20.0%
!!! ATTTATTTCATGGATCTTTT+TGG + chr4.2:14110619-14110638 MS.gene08541:intron 20.0%
!!! CAGTTTTACAATAATGATTA+CGG - chr4.2:14110241-14110260 None:intergenic 20.0%
!!! GTCATTTTTCATTTATTTCA+TGG + chr4.2:14110609-14110628 MS.gene08541:intron 20.0%
!!! TTTTAACTTCTTGTAAATAC+TGG + chr4.2:14110587-14110606 MS.gene08541:intron 20.0%
! AAACTTAGTAGAAAATCATG+AGG - chr4.2:14111338-14111357 None:intergenic 25.0%
! AATCAAATAACAAGCAGAAA+CGG - chr4.2:14110339-14110358 None:intergenic 25.0%
! ACTTTCAAATGCAAATTTAC+TGG - chr4.2:14109600-14109619 None:intergenic 25.0%
! AGAAAAATAAGTATTACCAC+AGG - chr4.2:14110297-14110316 None:intergenic 25.0%
! GAAAAATAAGTATTACCACA+GGG - chr4.2:14110296-14110315 None:intergenic 25.0%
! GATTAAGCATCTTTATCTTT+AGG - chr4.2:14110664-14110683 None:intergenic 25.0%
! GATTAGAAGATAAAAGAGAA+TGG - chr4.2:14111054-14111073 None:intergenic 25.0%
!! AGGTAATTCAATTTTACCAT+TGG - chr4.2:14109636-14109655 None:intergenic 25.0%
!! ATTTTCAGTAGCTTTTGTAT+CGG - chr4.2:14109402-14109421 None:intergenic 25.0%
!! TCAAGAGAAAATCTTTTGTT+TGG - chr4.2:14111178-14111197 None:intergenic 25.0%
!!! AACTTGCTTTTTTTTTTGGA+TGG + chr4.2:14110091-14110110 MS.gene08541:intron 25.0%
!!! AAGAGAAAATCTTTTGTTTG+GGG - chr4.2:14111176-14111195 None:intergenic 25.0%
!!! ATCTAAATGTTCTAGAGAAA+CGG - chr4.2:14111632-14111651 None:intergenic 25.0%
!!! CAAGAGAAAATCTTTTGTTT+GGG - chr4.2:14111177-14111196 None:intergenic 25.0%
!!! TAATTTTTGGTTCTACATCA+GGG + chr4.2:14109522-14109541 MS.gene08541:CDS 25.0%
!!! TGTATAGTGGATTTACTTAA+AGG + chr4.2:14110806-14110825 MS.gene08541:intron 25.0%
!!! TTCTGCTTGTTATTTGATTT+AGG + chr4.2:14110340-14110359 MS.gene08541:intron 25.0%
AAAAATTACAACCACCAAAC+TGG + chr4.2:14109423-14109442 MS.gene08541:CDS 30.0%
AAACAGTTATCGTGATTGTT+TGG + chr4.2:14110836-14110855 MS.gene08541:intron 30.0%
AAACCATCTATAACTACCAT+AGG - chr4.2:14109914-14109933 None:intergenic 30.0%
AAATGAATAACACTGTGCAA+GGG - chr4.2:14111485-14111504 None:intergenic 30.0%
AACTAAAATTGGCTACTCTT+CGG - chr4.2:14110970-14110989 None:intergenic 30.0%
ATAATAACCATGCAGAGTAA+TGG + chr4.2:14110739-14110758 MS.gene08541:intron 30.0%
CAGTCTTTGAATAAGCAAAT+GGG - chr4.2:14111112-14111131 None:intergenic 30.0%
TACCTATAAAGTTAGTAAGC+AGG - chr4.2:14110498-14110517 None:intergenic 30.0%
TATAACTACCATAGGAGATT+TGG - chr4.2:14109906-14109925 None:intergenic 30.0%
TATGGTAGTTATAGATGGTT+TGG + chr4.2:14109913-14109932 MS.gene08541:CDS 30.0%
TCATATTAGAGTTGCTAGAA+GGG + chr4.2:14110762-14110781 MS.gene08541:intron 30.0%
TGATGTTCCTAAAACTAGAT+TGG + chr4.2:14110874-14110893 MS.gene08541:intron 30.0%
TGTTTGGATCTAATTGACAT+AGG + chr4.2:14109990-14110009 MS.gene08541:intron 30.0%
TTAACAACGGTATGATTGTT+TGG + chr4.2:14109974-14109993 MS.gene08541:intron 30.0%
TTACTCTAATCAATTACACC+TGG - chr4.2:14111436-14111455 None:intergenic 30.0%
TTATCTTATTCGTGTCACAA+AGG - chr4.2:14110686-14110705 None:intergenic 30.0%
TTCCTGCTTACTAACTTTAT+AGG + chr4.2:14110493-14110512 MS.gene08541:intron 30.0%
TTTACCATTGGAATCATCAT+GGG - chr4.2:14109624-14109643 None:intergenic 30.0%
TTTGGATGTAAAATGGACAT+CGG - chr4.2:14111258-14111277 None:intergenic 30.0%
! AATAATGTTTTGCCGATGAA+AGG - chr4.2:14109853-14109872 None:intergenic 30.0%
! ACAACCAGACATTATTTTCT+CGG - chr4.2:14109463-14109482 None:intergenic 30.0%
! TCAATCTAAAACTCGGTAAA+AGG + chr4.2:14109720-14109739 MS.gene08541:CDS 30.0%
! TTTTACCATTGGAATCATCA+TGG - chr4.2:14109625-14109644 None:intergenic 30.0%
! TTTTCAGTAGCTTTTGTATC+GGG - chr4.2:14109401-14109420 None:intergenic 30.0%
!! ACAAAGATGATTGACTTGAT+TGG - chr4.2:14110265-14110284 None:intergenic 30.0%
!! AGAGAAAATCTTTTGTTTGG+GGG - chr4.2:14111175-14111194 None:intergenic 30.0%
!! CAAAGATGATTGACTTGATT+GGG - chr4.2:14110264-14110283 None:intergenic 30.0%
!! TTGATTTAGGTTGTTAAACC+TGG + chr4.2:14110353-14110372 MS.gene08541:intron 30.0%
!!! CTAATTTTTGGTTCTACATC+AGG + chr4.2:14109521-14109540 MS.gene08541:CDS 30.0%
!!! GACTTCTAGATTTTTTTTCC+AGG - chr4.2:14110374-14110393 None:intergenic 30.0%
AAATCTTCTACACCTGCAAA+AGG - chr4.2:14111517-14111536 None:intergenic 35.0%
AAGCAGAAACGGATATAAAC+TGG - chr4.2:14110328-14110347 None:intergenic 35.0%
AAGCCTATAAACGACAACAT+TGG + chr4.2:14111197-14111216 MS.gene08541:CDS 35.0%
AATATGACCATTACTCTGCA+TGG - chr4.2:14110749-14110768 None:intergenic 35.0%
AATCAGTTCATCACCAACTA+GGG + chr4.2:14109562-14109581 MS.gene08541:CDS 35.0%
AGATCATTCCAAATCTCCTA+TGG + chr4.2:14109895-14109914 MS.gene08541:CDS 35.0%
CAAATGAATAACACTGTGCA+AGG - chr4.2:14111486-14111505 None:intergenic 35.0%
CAAGGACTTTGGATGTAAAA+TGG - chr4.2:14111265-14111284 None:intergenic 35.0%
CACTTTGCTTAAAATGCTGA+AGG + chr4.2:14110141-14110160 MS.gene08541:intron 35.0%
CAGGTAAAGCTTCTTAACAA+CGG + chr4.2:14109961-14109980 MS.gene08541:intron 35.0%
CATCAAACTCAAACAGATCA+TGG - chr4.2:14110169-14110188 None:intergenic 35.0%
GATAGCATAGTTACAGTACT+TGG - chr4.2:14111227-14111246 None:intergenic 35.0%
GCAGTCTTTGAATAAGCAAA+TGG - chr4.2:14111113-14111132 None:intergenic 35.0%
GTCATATTAGAGTTGCTAGA+AGG + chr4.2:14110761-14110780 MS.gene08541:intron 35.0%
TAGACCGAGAAAATAATGTC+TGG + chr4.2:14109456-14109475 MS.gene08541:CDS 35.0%
TCTAATTCGATACTCCAGTT+TGG - chr4.2:14109440-14109459 None:intergenic 35.0%
TCTCCTATGGTAGTTATAGA+TGG + chr4.2:14109908-14109927 MS.gene08541:CDS 35.0%
! AGTGTCTATTGTAACTTGCA+AGG - chr4.2:14110522-14110541 None:intergenic 35.0%
! CATGATCTGTTTGAGTTTGA+TGG + chr4.2:14110167-14110186 MS.gene08541:CDS 35.0%
! CCATTGCTCAATCTAAAACT+CGG + chr4.2:14109713-14109732 MS.gene08541:CDS 35.0%
! CTAGATACACAAGAAAGCAT+TGG - chr4.2:14109689-14109708 None:intergenic 35.0%
! CTTGCACAGTGTTATTCATT+TGG + chr4.2:14111484-14111503 MS.gene08541:intron 35.0%
! GTTTTGCTAATATCCCTAGT+TGG - chr4.2:14109578-14109597 None:intergenic 35.0%
! TTTGGTAGAATACCTTTTGC+AGG + chr4.2:14111502-14111521 MS.gene08541:intron 35.0%
!! ACATTGTAAGTTCTGAGCAT+TGG - chr4.2:14109795-14109814 None:intergenic 35.0%
!! ATGTTCTAGAGAAACGGATT+TGG - chr4.2:14111626-14111645 None:intergenic 35.0%
!! GCTGTTTTGTATGTTTTGCA+AGG - chr4.2:14111147-14111166 None:intergenic 35.0%
!!! ACTCACACCAATCTAGTTTT+AGG - chr4.2:14110884-14110903 None:intergenic 35.0%
AATTCGATACTCCAGTTTGG+TGG - chr4.2:14109437-14109456 None:intergenic 40.0%
ACTGTGCCAATATTCTGATG+AGG - chr4.2:14111713-14111732 None:intergenic 40.0%
AGTTCCCATGATGATTCCAA+TGG + chr4.2:14109617-14109636 MS.gene08541:CDS 40.0%
ATGCAAAATCTCCCTCTTCA+CGG + chr4.2:14109351-14109370 MS.gene08541:CDS 40.0%
CAATATTCTGATGAGGCTAG+AGG - chr4.2:14111706-14111725 None:intergenic 40.0%
CAATCAGTTCATCACCAACT+AGG + chr4.2:14109561-14109580 MS.gene08541:CDS 40.0%
CATGGTGGTAGCAATCAATA+TGG - chr4.2:14111404-14111423 None:intergenic 40.0%
CATTGATCCTCTTGGTTTCA+AGG - chr4.2:14111283-14111302 None:intergenic 40.0%
CGGTAAAAGGATGAAATTCG+AGG + chr4.2:14109733-14109752 MS.gene08541:CDS 40.0%
CGTCATCTTTAGTTGTTGGA+AGG + chr4.2:14109759-14109778 MS.gene08541:CDS 40.0%
CTCTAGCCTCATCAGAATAT+TGG + chr4.2:14111704-14111723 MS.gene08541:CDS 40.0%
GGTGCATTAGAAGCTTCAAT+AGG - chr4.2:14109668-14109687 None:intergenic 40.0%
TACACCTGGCAATATTTGCA+TGG - chr4.2:14111422-14111441 None:intergenic 40.0%
! AGGGATTGCTATTAGAGTTG+TGG + chr4.2:14110781-14110800 MS.gene08541:intron 40.0%
! GGGATTGCTATTAGAGTTGT+GGG + chr4.2:14110782-14110801 MS.gene08541:intron 40.0%
! GTAGCTTTTGTATCGGGTAA+TGG - chr4.2:14109395-14109414 None:intergenic 40.0%
! GTGCCAATGTTGTCGTTTAT+AGG - chr4.2:14111203-14111222 None:intergenic 40.0%
!! AATATGTCGATTCCAGCATG+TGG + chr4.2:14111743-14111762 MS.gene08541:CDS 40.0%
!! CTTTTGTATCGGGTAATGGT+AGG - chr4.2:14109391-14109410 None:intergenic 40.0%
!!! CCGAGTTTTAGATTGAGCAA+TGG - chr4.2:14109716-14109735 None:intergenic 40.0%
!!! TCTGGCTCTGAGCTAATTTT+TGG + chr4.2:14109509-14109528 MS.gene08541:CDS 40.0%
AAAATCTCCCTCTTCACGGT+GGG + chr4.2:14109355-14109374 MS.gene08541:CDS 45.0%
ACCACCATGCAAATATTGCC+AGG + chr4.2:14111415-14111434 MS.gene08541:intron 45.0%
ACCTGGCAATATTTGCATGG+TGG - chr4.2:14111419-14111438 None:intergenic 45.0%
ACTGGTGAAAACACGACTCA+AGG - chr4.2:14111828-14111847 None:intergenic 45.0%
AGCTTTACCTGCTACATTGC+TGG - chr4.2:14109952-14109971 None:intergenic 45.0%
CAACTATAGCCTGCATAGCA+TGG - chr4.2:14110402-14110421 None:intergenic 45.0%
CCAAAGTCCTTGAAACCAAG+AGG + chr4.2:14111273-14111292 MS.gene08541:CDS 45.0%
CCAACTGGAAGTGGTTATTC+TGG + chr4.2:14109491-14109510 MS.gene08541:CDS 45.0%
CCAGAATAACCACTTCCAGT+TGG - chr4.2:14109494-14109513 None:intergenic 45.0%
CCTCTTGGTTTCAAGGACTT+TGG - chr4.2:14111276-14111295 None:intergenic 45.0%
GAAACCAAGAGGATCAATGC+AGG + chr4.2:14111284-14111303 MS.gene08541:CDS 45.0%
GACGCGTCATCTTTAGTTGT+TGG + chr4.2:14109755-14109774 MS.gene08541:CDS 45.0%
GCTTCAATAGGCCCTTCTTT+AGG - chr4.2:14109656-14109675 None:intergenic 45.0%
TTGGTCGCATATGAATCCCA+CGG - chr4.2:14111607-14111626 None:intergenic 45.0%
! AGTCTAAGACCATGCTATGC+AGG + chr4.2:14110390-14110409 MS.gene08541:intron 45.0%
! GATTTTGCCAGCAATGTAGC+AGG + chr4.2:14109942-14109961 MS.gene08541:CDS 45.0%
! GTTTTGCCGATGAAAGGTCA+AGG - chr4.2:14109847-14109866 None:intergenic 45.0%
! TAGAGTTGTGGGCTGTATAG+TGG + chr4.2:14110793-14110812 MS.gene08541:intron 45.0%
! TGAGTCGTGTTTTCACCAGT+TGG + chr4.2:14111828-14111847 MS.gene08541:CDS 45.0%
! TGAGTTTGAGATCCACATGC+TGG - chr4.2:14111758-14111777 None:intergenic 45.0%
! TTGTGGCCTTGACCTTTCAT+CGG + chr4.2:14109838-14109857 MS.gene08541:CDS 45.0%
CAAAATCTCCCTCTTCACGG+TGG + chr4.2:14109354-14109373 MS.gene08541:CDS 50.0%
TCTTCTGTCAACCAGTCCGT+GGG + chr4.2:14111588-14111607 MS.gene08541:CDS 50.0%
TCTTTGTCTTCGTCACCCTG+TGG + chr4.2:14110278-14110297 MS.gene08541:CDS 50.0%
TGGTTGTTCGATACGCCAAC+TGG + chr4.2:14109476-14109495 MS.gene08541:CDS 50.0%
TGTGTGTGCTATGGCTTGAG+CGG - chr4.2:14111668-14111687 None:intergenic 50.0%
TTCGATACGCCAACTGGAAG+TGG + chr4.2:14109482-14109501 MS.gene08541:CDS 50.0%
! CTGACCTGCATTGATCCTCT+TGG - chr4.2:14111291-14111310 None:intergenic 50.0%
! CTGACTCGTTGTGTGTGCTA+TGG - chr4.2:14111677-14111696 None:intergenic 50.0%
! TGACAGAAGAGAGGAAGCAC+AGG - chr4.2:14111578-14111597 None:intergenic 50.0%
ATCCCATTCCCACCGTGAAG+AGG - chr4.2:14109366-14109385 None:intergenic 55.0%
CAACCTTCAACCTGGCAGAG+AGG - chr4.2:14109821-14109840 None:intergenic 55.0%
CAAGGCCACAACCTTCAACC+TGG - chr4.2:14109829-14109848 None:intergenic 55.0%
CGCATATGAATCCCACGGAC+TGG - chr4.2:14111602-14111621 None:intergenic 55.0%
CTCTTCTGTCAACCAGTCCG+TGG + chr4.2:14111587-14111606 MS.gene08541:CDS 55.0%
TCCCATTCCCACCGTGAAGA+GGG - chr4.2:14109365-14109384 None:intergenic 55.0%
TCCCTCTTCACGGTGGGAAT+GGG + chr4.2:14109361-14109380 MS.gene08541:CDS 55.0%
TGCTATGGCTTGAGCGGTAC+TGG - chr4.2:14111662-14111681 None:intergenic 55.0%
! CTCTGCCAGGTTGAAGGTTG+TGG + chr4.2:14109821-14109840 MS.gene08541:CDS 55.0%
!! CGGACTGGTTGACAGAAGAG+AGG - chr4.2:14111587-14111606 None:intergenic 55.0%
ACAATGTCCGCCTCTCTGCC+AGG + chr4.2:14109808-14109827 MS.gene08541:CDS 60.0%
CCTTCAACCTGGCAGAGAGG+CGG - chr4.2:14109818-14109837 None:intergenic 60.0%
CTCCCTCTTCACGGTGGGAA+TGG + chr4.2:14109360-14109379 MS.gene08541:CDS 60.0%
! CCGCCTCTCTGCCAGGTTGA+AGG + chr4.2:14109815-14109834 MS.gene08541:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 14109341 14111855 14109341 ID=MS.gene08541
chr4.2 mRNA 14109341 14111855 14109341 ID=MS.gene08541.t1;Parent=MS.gene08541
chr4.2 exon 14109341 14109963 14109341 ID=MS.gene08541.t1.exon1;Parent=MS.gene08541.t1
chr4.2 CDS 14109341 14109963 14109341 ID=cds.MS.gene08541.t1;Parent=MS.gene08541.t1
chr4.2 exon 14110163 14110299 14110163 ID=MS.gene08541.t1.exon2;Parent=MS.gene08541.t1
chr4.2 CDS 14110163 14110299 14110163 ID=cds.MS.gene08541.t1;Parent=MS.gene08541.t1
chr4.2 exon 14111090 14111305 14111090 ID=MS.gene08541.t1.exon3;Parent=MS.gene08541.t1
chr4.2 CDS 14111090 14111305 14111090 ID=cds.MS.gene08541.t1;Parent=MS.gene08541.t1
chr4.2 exon 14111524 14111855 14111524 ID=MS.gene08541.t1.exon4;Parent=MS.gene08541.t1
chr4.2 CDS 14111524 14111855 14111524 ID=cds.MS.gene08541.t1;Parent=MS.gene08541.t1
Gene Sequence

>MS.gene08541

ATGGATTTGGATGCAAAATCTCCCTCTTCACGGTGGGAATGGGATAGCCTACCATTACCCGATACAAAAGCTACTGAAAATAAAAAATTACAACCACCAAACTGGAGTATCGAATTAGACCGAGAAAATAATGTCTGGTTGTTCGATACGCCAACTGGAAGTGGTTATTCTGGCTCTGAGCTAATTTTTGGTTCTACATCAGGGAGTTCGAAATCAGATTCAATCAGTTCATCACCAACTAGGGATATTAGCAAAACCAGTAAATTTGCATTTGAAAGTTCCCATGATGATTCCAATGGTAAAATTGAATTACCTAAAGAAGGGCCTATTGAAGCTTCTAATGCACCAATGCTTTCTTGTGTATCTAGTGAACCATTGCTCAATCTAAAACTCGGTAAAAGGATGAAATTCGAGGACGCGTCATCTTTAGTTGTTGGAAGGAAATGTAAGTCCAATGCTCAGAACTTACAATGTCCGCCTCTCTGCCAGGTTGAAGGTTGTGGCCTTGACCTTTCATCGGCAAAACATTATTATCGCAAACGAAGAGTTTGTGTAGATCATTCCAAATCTCCTATGGTAGTTATAGATGGTTTGGAACGACGATTTTGCCAGCAATGTAGCAGGTAAAGCTTCTTAACAACGGTATGATTGTTTGGATCTAATTGACATAGGAAATAATAGTATTATTTTGGAATGATGAATTCTTGCTAGCACATCATTAGAAGTTAATATTACTACTCAATCTGATTAAACTTGCTTTTTTTTTTGGATGGTGTTGAATAAATTGCTCTAGAATTGATCACTTTGCTTAAAATGCTGAAGGTTCCATGATCTGTTTGAGTTTGATGGAAAGAAGAAAAGCTGCAGACGACGTCTTTCACAACACAATGCATTGCGCCGTAATCATTATTGTAAAACTGCCCAATCAAGTCAATCATCTTTGTCTTCGTCACCCTGTGGTAATACTTATTTTTCTTTATTTTTCCAGTTTATATCCGTTTCTGCTTGTTATTTGATTTAGGTTGTTAAACCTGGAAAAAAAATCTAGAAGTCTAAGACCATGCTATGCAGGCTATAGTTGAGTATTAACATTGCTGAATGAAGATTGTTTGAAAAACTTAAATACTTGACATCACTTTTCTTTTCTTTGCTTTCCTGCTTACTAACTTTATAGGTAACCTTGCAAGTTACAATAGACACTAATGTCATGTGACAAAACTTCTTAATTTTCTACAATTTTCTATCTTTTTAACTTCTTGTAAATACTGGTCATTTTTCATTTATTTCATGGATCTTTTTGGATTTTCAATTGTCATACATCCTAAAGATAAAGATGCTTAATCCTTTGTGACACGAATAAGATAAAGCATGTTCAAGAGTTCTAGCAGTATCAATTTCATAATAACCATGCAGAGTAATGGTCATATTAGAGTTGCTAGAAGGGATTGCTATTAGAGTTGTGGGCTGTATAGTGGATTTACTTAAAGGCACAAGAAAACAGTTATCGTGATTGTTTGGACTTGAACGCGAGTATGATGTTCCTAAAACTAGATTGGTGTGAGTATGAAATTCATTAAAACCTCATGTAATAAAAATTCAACTTCAAGCTGTTAGTCAAAACTTATACCGAAGAGTAGCCAATTTTAGTTCATTTATGTTTATTATAGTTACTTTCAAATCATTCATTTCACGATAATGATGCAAATTTTACCATTCTCTTTTATCTTCTAATCTATTTTAGTTCTGCAGATGAGAAGCAACAGATGAACCCATTTGCTTATTCAAAGACTGCTATAAATTTAGCCTTGCAAAACATACAAAACAGCGAGCTCCCCCAAACAAAAGATTTTCTCTTGAAGCCTATAAACGACAACATTGGCACACCAAGTACTGTAACTATGCTATCTGATGATTCCGATGTCCATTTTACATCCAAAGTCCTTGAAACCAAGAGGATCAATGCAGGTCAGTTCACGTCTTACTTTAAAATTAAGCCTCATGATTTTCTACTAAGTTTAGATTGTTACATTTATCATGTAAACATAATCTATTTATTTTGGCCATATTGATTGCTACCACCATGCAAATATTGCCAGGTGTAATTGATTAGAGTAATATGGTATTGAAGTATGTAATAATATCCCTTGCACAGTGTTATTCATTTGGTAGAATACCTTTTGCAGGTGTAGAAGATTTCATTAGCTTCTCTGATACAGATGCTACACAAGACTTCACCTGTGCTTCCTCTCTTCTGTCAACCAGTCCGTGGGATTCATATGCGACCAAATCCGTTTCTCTAGAACATTTAGATTGTATAACCAGTACCGCTCAAGCCATAGCACACACAACGAGTCAGTGTATGCCTCTAGCCTCATCAGAATATTGGCACAGTGTTCTTGATCAATATGTCGATTCCAGCATGTGGATCTCAAACTCAGACTGTGAAGAAAGCGATCATTTTCAAGAATTTCAATGTTCTGAGAACCTTGAGTCGTGTTTTCACCAGTTGGACTAA
Protein sequence

>MS.gene08541.t1

MDLDAKSPSSRWEWDSLPLPDTKATENKKLQPPNWSIELDRENNVWLFDTPTGSGYSGSELIFGSTSGSSKSDSISSSPTRDISKTSKFAFESSHDDSNGKIELPKEGPIEASNAPMLSCVSSEPLLNLKLGKRMKFEDASSLVVGRKCKSNAQNLQCPPLCQVEGCGLDLSSAKHYYRKRRVCVDHSKSPMVVIDGLERRFCQQCSRFHDLFEFDGKKKSCRRRLSQHNALRRNHYCKTAQSSQSSLSSSPCDEKQQMNPFAYSKTAINLALQNIQNSELPQTKDFLLKPINDNIGTPSTVTMLSDDSDVHFTSKVLETKRINAGVEDFISFSDTDATQDFTCASSLLSTSPWDSYATKSVSLEHLDCITSTAQAIAHTTSQCMPLASSEYWHSVLDQYVDSSMWISNSDCEESDHFQEFQCSENLESCFHQLD