AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene08996

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08996.t1 MTR_8g081000 78.629 248 52 1 28 274 139 386 4.17e-136 390
MS.gene08996.t1 MTR_8g081000 78.629 248 52 1 28 274 139 386 6.51e-136 390
MS.gene08996.t1 MTR_8g081000 78.629 248 52 1 28 274 139 386 1.13e-135 389
MS.gene08996.t1 MTR_8g080990 77.419 248 55 1 28 274 126 373 5.14e-135 387
MS.gene08996.t1 MTR_3g450500 27.237 257 180 3 25 274 115 371 1.80e-26 107
MS.gene08996.t1 MTR_2g437150 29.839 248 169 3 35 278 147 393 2.22e-24 101
MS.gene08996.t1 MTR_2g437960 27.917 240 170 2 35 272 145 383 3.14e-24 101
MS.gene08996.t1 MTR_2g437960 27.917 240 170 2 35 272 145 383 3.34e-24 101
MS.gene08996.t1 MTR_2g437160 28.750 240 169 2 35 273 155 393 7.37e-24 100
MS.gene08996.t1 MTR_2g437200 29.461 241 167 3 35 273 157 396 2.13e-23 99.0
MS.gene08996.t1 MTR_2g436400 26.360 239 174 2 35 273 139 375 2.70e-23 98.2
MS.gene08996.t1 MTR_2g437100 29.630 243 166 3 35 273 152 393 2.45e-22 95.9
MS.gene08996.t1 MTR_2g076320 28.333 240 169 2 35 272 142 380 2.90e-22 95.5
MS.gene08996.t1 MTR_2g437240 29.297 256 176 3 28 279 150 404 5.51e-22 95.1
MS.gene08996.t1 MTR_2g019840 28.916 249 169 6 28 273 126 369 6.27e-22 94.7
MS.gene08996.t1 MTR_2g437080 29.675 246 171 2 34 279 131 374 6.66e-22 94.4
MS.gene08996.t1 MTR_2g437060 27.755 245 169 4 37 278 149 388 1.73e-21 93.6
MS.gene08996.t1 MTR_2g437170 28.807 243 168 3 35 273 147 388 2.15e-21 93.2
MS.gene08996.t1 MTR_2g019810 28.112 249 171 6 28 273 114 357 3.19e-21 92.4
MS.gene08996.t1 MTR_4g119580 28.395 243 166 3 35 274 134 371 8.69e-21 91.3
MS.gene08996.t1 MTR_2g437990 26.667 240 172 3 35 272 142 379 9.93e-21 91.7
MS.gene08996.t1 MTR_2g436460 26.141 241 171 4 35 272 137 373 1.78e-20 90.5
MS.gene08996.t1 MTR_2g437040 27.126 247 174 3 35 279 132 374 2.30e-20 90.1
MS.gene08996.t1 MTR_2g437120 28.216 241 169 3 35 273 143 381 2.87e-20 90.1
MS.gene08996.t1 MTR_2g438010 25.100 251 185 2 24 272 151 400 6.94e-20 89.0
MS.gene08996.t1 MTR_2g437130 28.000 250 170 3 28 273 126 369 8.00e-20 88.6
MS.gene08996.t1 MTR_2g437030 26.860 242 175 2 37 278 220 459 2.44e-18 84.7
MS.gene08996.t1 MTR_2g438020 25.410 244 179 3 28 269 189 431 6.70e-18 83.6
MS.gene08996.t1 MTR_4g119550 24.400 250 175 3 35 278 142 383 6.75e-18 83.2
MS.gene08996.t1 MTR_4g007490 26.316 247 156 5 42 273 133 368 8.62e-18 82.8
MS.gene08996.t1 MTR_4g120380 24.066 241 181 2 35 275 134 372 1.18e-17 82.4
MS.gene08996.t1 MTR_2g437160 27.363 201 131 1 88 273 102 302 1.32e-17 81.6
MS.gene08996.t1 MTR_2g437180 26.721 247 176 4 28 272 134 377 5.81e-17 80.5
MS.gene08996.t1 MTR_2g437020 26.800 250 166 4 35 272 131 375 1.82e-15 76.3
MS.gene08996.t1 MTR_4g120380 23.744 219 165 2 35 253 134 350 1.71e-14 73.6
MS.gene08996.t1 MTR_2g437940 30.822 146 101 0 128 273 207 352 3.27e-14 72.4
MS.gene08996.t1 MTR_2g436440 23.577 246 184 3 35 279 138 380 1.00e-13 70.9
MS.gene08996.t1 MTR_2g437260 20.675 237 184 3 38 272 129 363 2.70e-13 69.7
MS.gene08996.t1 MTR_2g437090 26.923 156 114 0 128 283 11 166 9.35e-12 62.8
MS.gene08996.t1 MTR_8g012210 30.667 150 99 3 127 273 201 348 3.77e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene08996.t1 AT5G64950 41.016 256 141 5 28 273 121 376 4.27e-56 186
MS.gene08996.t1 AT5G07900 27.600 250 177 3 29 274 152 401 4.12e-26 106
MS.gene08996.t1 AT1G21150 28.049 246 171 4 29 269 103 347 6.70e-26 105
MS.gene08996.t1 AT1G21150 28.049 246 171 4 29 269 139 383 9.59e-26 105
MS.gene08996.t1 AT1G21150 28.049 246 171 4 29 269 203 447 1.99e-25 105
MS.gene08996.t1 AT1G61990 23.667 300 185 5 22 277 115 414 5.44e-16 77.8
MS.gene08996.t1 AT1G61990 23.667 300 185 5 22 277 115 414 5.44e-16 77.8
MS.gene08996.t1 AT1G62010 26.923 234 132 4 85 279 174 407 5.95e-15 74.7
MS.gene08996.t1 AT1G61970 33.636 110 69 2 153 258 290 399 3.46e-11 63.5
MS.gene08996.t1 AT1G61970 33.636 110 69 2 153 258 290 399 3.46e-11 63.5
MS.gene08996.t1 AT1G61970 33.636 110 69 2 153 258 290 399 3.46e-11 63.5
MS.gene08996.t1 AT1G61970 33.636 110 69 2 153 258 290 399 3.46e-11 63.5
MS.gene08996.t1 AT1G61970 33.636 110 69 2 153 258 290 399 3.46e-11 63.5
MS.gene08996.t1 AT1G61970 33.636 110 69 2 153 258 290 399 3.46e-11 63.5
MS.gene08996.t1 AT1G61970 33.636 110 69 2 153 258 290 399 3.46e-11 63.5
MS.gene08996.t1 AT1G61960 26.374 182 130 2 97 274 272 453 5.19e-11 63.2

Find 47 sgRNAs with CRISPR-Local

Find 159 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTGATACCCTAAGCAAATT+TGG 0.167535 4.4:-15077006 None:intergenic
CTTGATTTCATTCAGTGTTT+TGG 0.182326 4.4:+15076943 MS.gene08996:CDS
GTTTGAGCCATAAAACATTT+AGG 0.239728 4.4:+15076914 MS.gene08996:CDS
GGTATCAGAGAAGAAGTTAA+AGG 0.274790 4.4:+15077020 MS.gene08996:CDS
GTTCAAACTAGCAGACAAAA+TGG 0.299580 4.4:-15076596 None:intergenic
TAGAGAGTTGTGGCATTGTT+GGG 0.299637 4.4:+15076749 MS.gene08996:CDS
GAGCTTGCAAATGTTCAAAA+AGG 0.301950 4.4:+15076981 MS.gene08996:CDS
TTTATCTGAGGACACATTCT+TGG 0.317933 4.4:+15077218 MS.gene08996:CDS
TTGTGGCATTGTTGGGGATC+AGG 0.331357 4.4:+15076756 MS.gene08996:CDS
AAAAGGTTCCAAATTTGCTT+AGG 0.354562 4.4:+15076998 MS.gene08996:CDS
CAAAGTCAGCATTGGTTAAT+CGG 0.362750 4.4:+15077079 MS.gene08996:CDS
CAATGTTGGCAACAAATGTT+TGG 0.383801 4.4:-15076720 None:intergenic
CGATTAACCAATGCTGACTT+TGG 0.389786 4.4:-15077078 None:intergenic
TTAGAGAGTTGTGGCATTGT+TGG 0.401579 4.4:+15076748 MS.gene08996:CDS
GAAGCTATTAAGAAAATTGT+AGG 0.405231 4.4:+15076640 MS.gene08996:CDS
CTCTCTCTCTCATCGTCCGC+CGG 0.405694 4.4:-15075637 None:intergenic
AAAGGTTCCAAATTTGCTTA+GGG 0.440166 4.4:+15076999 MS.gene08996:CDS
TTTAACACATAAAGAAAGCT+TGG 0.468094 4.4:-15077195 None:intergenic
AAACTAGCAGACAAAATGGT+TGG 0.477531 4.4:-15076592 None:intergenic
TTCTTAATAGCTTCCACAGA+TGG 0.480816 4.4:-15076631 None:intergenic
TCAGAGAAGAAGTTAAAGGT+TGG 0.481727 4.4:+15077024 MS.gene08996:CDS
GCAACAAATGTTTGGTACAC+TGG 0.495821 4.4:-15076712 None:intergenic
TCTCTCTCTCATCGTCCGCC+GGG 0.497642 4.4:-15075636 None:intergenic
CATTATTGATAGCTTACAAA+GGG 0.508791 4.4:+15077277 MS.gene08996:CDS
ATCACAAAATCTATGTAAGA+AGG 0.510006 4.4:+15077170 MS.gene08996:CDS
GTGGACGGTGATGACGGTCC+CGG 0.515221 4.4:+15075618 MS.gene08996:CDS
ACTCTCTAAGAAAGCAATGT+TGG 0.518565 4.4:-15076734 None:intergenic
AGAGAGTTGTGGCATTGTTG+GGG 0.519144 4.4:+15076750 MS.gene08996:CDS
TGGATGGAGTTTATAAGAGA+AGG 0.526403 4.4:+15075590 MS.gene08996:CDS
TCTTAATAGCTTCCACAGAT+GGG 0.550512 4.4:-15076630 None:intergenic
ATTGCTTTCTTAGAGAGTTG+TGG 0.553536 4.4:+15076739 MS.gene08996:CDS
GAGTTTATAAGAGAAGGAAG+TGG 0.563117 4.4:+15075596 MS.gene08996:CDS
TGCTAGTTTGAACAAAACAT+TGG 0.566720 4.4:+15076606 MS.gene08996:CDS
CAAAACATTGGTCCCATCTG+TGG 0.566916 4.4:+15076618 MS.gene08996:CDS
TTATGTGTTAAATTTATCTG+AGG 0.573104 4.4:+15077206 MS.gene08996:CDS
TATGCTGCCAAAGTCAGCAT+TGG 0.579046 4.4:+15077071 MS.gene08996:CDS
TTTATAAGAGAAGGAAGTGG+TGG 0.587567 4.4:+15075599 MS.gene08996:CDS
AGAGCGTCAAGAGATCATTA+CGG 0.590751 4.4:-15077313 None:intergenic
GAAGTGGTGGACGGTGATGA+CGG 0.593945 4.4:+15075612 MS.gene08996:CDS
AAGCTATTAAGAAAATTGTA+GGG 0.601947 4.4:+15076641 MS.gene08996:CDS
TAAGAGAAGGAAGTGGTGGA+CGG 0.605323 4.4:+15075603 MS.gene08996:CDS
TAGTTTCTAATACGAGATTG+TGG 0.627705 4.4:-15076817 None:intergenic
GCATTATTGATAGCTTACAA+AGG 0.630637 4.4:+15077276 MS.gene08996:CDS
ATGCTATACATCAAAATCAG+AGG 0.630674 4.4:-15077102 None:intergenic
AAAGTTTAAAGAAAATGCAG+AGG 0.668498 4.4:+15077254 MS.gene08996:CDS
GACGGTGATGACGGTCCCGG+CGG 0.702651 4.4:+15075621 MS.gene08996:CDS
ATTATTGATAGCTTACAAAG+GGG 0.710219 4.4:+15077278 MS.gene08996:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTAAATACATAATATTATG+CGG + chr4.4:15075763-15075782 MS.gene08996:intron 10.0%
!!! AAAATAAAGGATTTTATTTT+AGG - chr4.4:15076109-15076128 None:intergenic 10.0%
!! AAGCTATTAAGAAAATTGTA+GGG + chr4.4:15076641-15076660 MS.gene08996:CDS 20.0%
!! AGAATAAGACATGAAAATAA+AGG - chr4.4:15076122-15076141 None:intergenic 20.0%
!! TTATGTGTTAAATTTATCTG+AGG + chr4.4:15077206-15077225 MS.gene08996:CDS 20.0%
!!! AAAAAGTTGATTACAGATAA+GGG - chr4.4:15076412-15076431 None:intergenic 20.0%
!!! AGATTTATGTAATTTGGAAT+TGG - chr4.4:15076339-15076358 None:intergenic 20.0%
!!! ATCAACTTTTTCAAACAAAT+TGG + chr4.4:15076421-15076440 MS.gene08996:intron 20.0%
! AAAGCTGAAAAACTCTAAAT+CGG - chr4.4:15077146-15077165 None:intergenic 25.0%
! AAAGTTTAAAGAAAATGCAG+AGG + chr4.4:15077254-15077273 MS.gene08996:CDS 25.0%
! AACAAGTTTAGATACATAGA+TGG - chr4.4:15076366-15076385 None:intergenic 25.0%
! AATACATAATATTATGCGGA+CGG + chr4.4:15075767-15075786 MS.gene08996:intron 25.0%
! AATAGAACAAACATAGATTC+TGG - chr4.4:15076052-15076071 None:intergenic 25.0%
! AGTTGAATTCTTTCAACAAT+TGG + chr4.4:15076528-15076547 MS.gene08996:intron 25.0%
! ATCACAAAATCTATGTAAGA+AGG + chr4.4:15077170-15077189 MS.gene08996:CDS 25.0%
! GAAGCTATTAAGAAAATTGT+AGG + chr4.4:15076640-15076659 MS.gene08996:CDS 25.0%
! GGATTCAGATTTATGTAATT+TGG - chr4.4:15076345-15076364 None:intergenic 25.0%
! GTTGAATTCTTTCAACAATT+GGG + chr4.4:15076529-15076548 MS.gene08996:intron 25.0%
! TCAATTTAACAAACATGTTG+CGG - chr4.4:15076157-15076176 None:intergenic 25.0%
! TGTTGAAAGAATTCAACTTT+AGG - chr4.4:15076526-15076545 None:intergenic 25.0%
! TTTAACACATAAAGAAAGCT+TGG - chr4.4:15077198-15077217 None:intergenic 25.0%
!! AGAATTCAACTTTAGGTTTA+AGG - chr4.4:15076519-15076538 None:intergenic 25.0%
!! ATTATTGATAGCTTACAAAG+GGG + chr4.4:15077278-15077297 MS.gene08996:CDS 25.0%
!! CATTATTGATAGCTTACAAA+GGG + chr4.4:15077277-15077296 MS.gene08996:CDS 25.0%
!! GAAAAAGTTGATTACAGATA+AGG - chr4.4:15076413-15076432 None:intergenic 25.0%
!! GAATTCAACTTTAGGTTTAA+GGG - chr4.4:15076518-15076537 None:intergenic 25.0%
!! GTAATTTGGAATTGGAATTA+AGG - chr4.4:15076331-15076350 None:intergenic 25.0%
!! TATGTATCTAAACTTGTTTC+AGG + chr4.4:15076368-15076387 MS.gene08996:intron 25.0%
!! TCTGCATTTTCTTTAAACTT+TGG - chr4.4:15077255-15077274 None:intergenic 25.0%
!! TTGATTTCATTCAGTGTTTT+GGG + chr4.4:15076944-15076963 MS.gene08996:CDS 25.0%
!!! GCTTTTTCCTAAATGTTTTA+TGG - chr4.4:15076924-15076943 None:intergenic 25.0%
!!! TTTATTTTAGGTTTGAAAGC+AGG - chr4.4:15076097-15076116 None:intergenic 25.0%
AAGAGAATGTAACAACTTGA+GGG - chr4.4:15076204-15076223 None:intergenic 30.0%
AAGCTGAAAAACTCTAAATC+GGG - chr4.4:15077145-15077164 None:intergenic 30.0%
ATACATAATATTATGCGGAC+GGG + chr4.4:15075768-15075787 MS.gene08996:intron 30.0%
ATGCTATACATCAAAATCAG+AGG - chr4.4:15077105-15077124 None:intergenic 30.0%
CAATTTAACAAACATGTTGC+GGG - chr4.4:15076156-15076175 None:intergenic 30.0%
GTTTGAGCCATAAAACATTT+AGG + chr4.4:15076914-15076933 MS.gene08996:CDS 30.0%
TAAACAGAAAAGTGGTTTCT+TGG - chr4.4:15076304-15076323 None:intergenic 30.0%
TAGTTTCTAATACGAGATTG+TGG - chr4.4:15076820-15076839 None:intergenic 30.0%
! AAAAGGTTCCAAATTTGCTT+AGG + chr4.4:15076998-15077017 MS.gene08996:CDS 30.0%
! AAAGGTTCCAAATTTGCTTA+GGG + chr4.4:15076999-15077018 MS.gene08996:CDS 30.0%
! ATTTGGAATTGGAATTAAGG+AGG - chr4.4:15076328-15076347 None:intergenic 30.0%
! CAAGCATTCTTGAATTTTGA+TGG - chr4.4:15076870-15076889 None:intergenic 30.0%
! CTTGATTTCATTCAGTGTTT+TGG + chr4.4:15076943-15076962 MS.gene08996:CDS 30.0%
! GCATTATTGATAGCTTACAA+AGG + chr4.4:15077276-15077295 MS.gene08996:CDS 30.0%
! GGTTTGAAGATTGGTTAATT+GGG - chr4.4:15076283-15076302 None:intergenic 30.0%
! GTTTGAAGATTGGTTAATTG+GGG - chr4.4:15076282-15076301 None:intergenic 30.0%
! GTTTTGCAAGCTGAAAAATT+GGG + chr4.4:15076841-15076860 MS.gene08996:CDS 30.0%
! TGCTAGTTTGAACAAAACAT+TGG + chr4.4:15076606-15076625 MS.gene08996:CDS 30.0%
! TGGTTTGAAGATTGGTTAAT+TGG - chr4.4:15076284-15076303 None:intergenic 30.0%
! TGTTTTGCAAGCTGAAAAAT+TGG + chr4.4:15076840-15076859 MS.gene08996:CDS 30.0%
! TTTCAACAATTGGGTTTTCA+GGG + chr4.4:15076538-15076557 MS.gene08996:intron 30.0%
!! TCTGATTTTGATGTATAGCA+TGG + chr4.4:15077104-15077123 MS.gene08996:CDS 30.0%
AAAATCCGCATGAAAAAAGG+AGG - chr4.4:15075904-15075923 None:intergenic 35.0%
AAACTAGCAGACAAAATGGT+TGG - chr4.4:15076595-15076614 None:intergenic 35.0%
AAGGAGGTTAAACAGAAAAG+TGG - chr4.4:15076312-15076331 None:intergenic 35.0%
AGCTGAAAAACTCTAAATCG+GGG - chr4.4:15077144-15077163 None:intergenic 35.0%
ATGGTCAAATCGGATATTAC+TGG - chr4.4:15075844-15075863 None:intergenic 35.0%
ATTACAGATAAGGGCTTTCA+GGG - chr4.4:15076403-15076422 None:intergenic 35.0%
ATTGCTTTCTTAGAGAGTTG+TGG + chr4.4:15076739-15076758 MS.gene08996:CDS 35.0%
CAATGTTGGCAACAAATGTT+TGG - chr4.4:15076723-15076742 None:intergenic 35.0%
GAAGAGAATGTAACAACTTG+AGG - chr4.4:15076205-15076224 None:intergenic 35.0%
GAGCTTGCAAATGTTCAAAA+AGG + chr4.4:15076981-15077000 MS.gene08996:CDS 35.0%
GAGTTTATAAGAGAAGGAAG+TGG + chr4.4:15075596-15075615 MS.gene08996:CDS 35.0%
GATTTCGAATCAAAAGCAGA+AGG - chr4.4:15076030-15076049 None:intergenic 35.0%
GGTATCAGAGAAGAAGTTAA+AGG + chr4.4:15077020-15077039 MS.gene08996:CDS 35.0%
GTTCAAACTAGCAGACAAAA+TGG - chr4.4:15076599-15076618 None:intergenic 35.0%
TCAGAGAAGAAGTTAAAGGT+TGG + chr4.4:15077024-15077043 MS.gene08996:CDS 35.0%
TCTGATACCCTAAGCAAATT+TGG - chr4.4:15077009-15077028 None:intergenic 35.0%
TCTTAATAGCTTCCACAGAT+GGG - chr4.4:15076633-15076652 None:intergenic 35.0%
TGGATGGAGTTTATAAGAGA+AGG + chr4.4:15075590-15075609 MS.gene08996:CDS 35.0%
TTCTTAATAGCTTCCACAGA+TGG - chr4.4:15076634-15076653 None:intergenic 35.0%
TTTATAAGAGAAGGAAGTGG+TGG + chr4.4:15075599-15075618 MS.gene08996:CDS 35.0%
TTTATCTGAGGACACATTCT+TGG + chr4.4:15077218-15077237 MS.gene08996:CDS 35.0%
! ACTCTCTAAGAAAGCAATGT+TGG - chr4.4:15076737-15076756 None:intergenic 35.0%
! CTTTCAACAATTGGGTTTTC+AGG + chr4.4:15076537-15076556 MS.gene08996:intron 35.0%
! GTATTTGAGTTCTCTGACAA+AGG - chr4.4:15076474-15076493 None:intergenic 35.0%
! TGGTTTCTTGGTTTGAAGAT+TGG - chr4.4:15076292-15076311 None:intergenic 35.0%
!! CAAAGTCAGCATTGGTTAAT+CGG + chr4.4:15077079-15077098 MS.gene08996:CDS 35.0%
!! GATATTACTGGTTTAGTGTG+GGG - chr4.4:15075832-15075851 None:intergenic 35.0%
!! GATTTTGATGTATAGCATGG+AGG + chr4.4:15077107-15077126 MS.gene08996:CDS 35.0%
!! GGATATTACTGGTTTAGTGT+GGG - chr4.4:15075833-15075852 None:intergenic 35.0%
!!! ATTCAAGTTTTGAGCTACTG+TGG + chr4.4:15076679-15076698 MS.gene08996:CDS 35.0%
!!! CTTTTTTCATGCGGATTTTG+CGG + chr4.4:15075905-15075924 MS.gene08996:intron 35.0%
!!! TTTTTTCATGCGGATTTTGC+GGG + chr4.4:15075906-15075925 MS.gene08996:intron 35.0%
AACAACTTGAGGGATTGCAT+TGG - chr4.4:15076194-15076213 None:intergenic 40.0%
ATACCAAGAAGGTCAATGCT+TGG - chr4.4:15075987-15076006 None:intergenic 40.0%
CGAAGTGAGTTAGAACTGAA+AGG - chr4.4:15076227-15076246 None:intergenic 40.0%
CGCAAAATCCGCATGAAAAA+AGG - chr4.4:15075907-15075926 None:intergenic 40.0%
CGCATGAAAAAAGGAGGAAA+CGG - chr4.4:15075898-15075917 None:intergenic 40.0%
GATTACAGATAAGGGCTTTC+AGG - chr4.4:15076404-15076423 None:intergenic 40.0%
GCAACAAATGTTTGGTACAC+TGG - chr4.4:15076715-15076734 None:intergenic 40.0%
TAGAGAGTTGTGGCATTGTT+GGG + chr4.4:15076749-15076768 MS.gene08996:CDS 40.0%
TGAAAAAAGGAGGAAACGGT+CGG - chr4.4:15075894-15075913 None:intergenic 40.0%
TTACAGATAAGGGCTTTCAG+GGG - chr4.4:15076402-15076421 None:intergenic 40.0%
TTAGAGAGTTGTGGCATTGT+TGG + chr4.4:15076748-15076767 MS.gene08996:CDS 40.0%
TTGAGTTCTCTGACAAAGGA+TGG - chr4.4:15076470-15076489 None:intergenic 40.0%
! TAATATTATGCGGACGGGTT+CGG + chr4.4:15075773-15075792 MS.gene08996:intron 40.0%
!! CGATTAACCAATGCTGACTT+TGG - chr4.4:15077081-15077100 None:intergenic 40.0%
!! CGGATATTACTGGTTTAGTG+TGG - chr4.4:15075834-15075853 None:intergenic 40.0%
!! GGTTTTCAGGGTTCTCAATT+AGG + chr4.4:15076550-15076569 MS.gene08996:intron 40.0%
!!! CGTTTCCTCCTTTTTTCATG+CGG + chr4.4:15075896-15075915 MS.gene08996:intron 40.0%
AGAAGGAGAAACCAGGATGA+GGG - chr4.4:15076013-15076032 None:intergenic 45.0%
AGAGAGAACTATGGTGAGTG+AGG + chr4.4:15075654-15075673 MS.gene08996:intron 45.0%
AGAGAGTTGTGGCATTGTTG+GGG + chr4.4:15076750-15076769 MS.gene08996:CDS 45.0%
CAAAACATTGGTCCCATCTG+TGG + chr4.4:15076618-15076637 MS.gene08996:CDS 45.0%
CAAAAGCAGAAGGAGAAACC+AGG - chr4.4:15076020-15076039 None:intergenic 45.0%
CGATGAGAGAGAGAGAACTA+TGG + chr4.4:15075645-15075664 MS.gene08996:CDS 45.0%
GATGAGGGAGAATACCAAGA+AGG - chr4.4:15075998-15076017 None:intergenic 45.0%
GGTTAGACGGATGGTCAAAT+CGG - chr4.4:15075854-15075873 None:intergenic 45.0%
TATGCTGCCAAAGTCAGCAT+TGG + chr4.4:15077071-15077090 MS.gene08996:CDS 45.0%
! ACTTGAGGGATTGCATTGGT+AGG - chr4.4:15076190-15076209 None:intergenic 45.0%
! TAAGAGAAGGAAGTGGTGGA+CGG + chr4.4:15075603-15075622 MS.gene08996:CDS 45.0%
!! ATTACTGGTTTAGTGTGGGG+CGG - chr4.4:15075829-15075848 None:intergenic 45.0%
!!! AAGTTTTGAGCTACTGTGGC+TGG + chr4.4:15076683-15076702 MS.gene08996:CDS 45.0%
!!! AGTTTTGAGCTACTGTGGCT+GGG + chr4.4:15076684-15076703 MS.gene08996:CDS 45.0%
CAGAAGGAGAAACCAGGATG+AGG - chr4.4:15076014-15076033 None:intergenic 50.0%
CCAACAGGCAACAACAACAC+TGG - chr4.4:15075688-15075707 None:intergenic 50.0%
CTTGGTATTCTCCCTCATCC+TGG + chr4.4:15075999-15076018 MS.gene08996:intron 50.0%
GAGTGTGAGGTGAGAGACAA+AGG + chr4.4:15075707-15075726 MS.gene08996:intron 50.0%
TTGTGGCATTGTTGGGGATC+AGG + chr4.4:15076756-15076775 MS.gene08996:CDS 50.0%
TTGTTGCCTGTTGGAGTGTG+AGG + chr4.4:15075694-15075713 MS.gene08996:intron 50.0%
! CCAGTGTTGTTGTTGCCTGT+TGG + chr4.4:15075685-15075704 MS.gene08996:intron 50.0%
! GGGATTGCATTGGTAGGACT+AGG - chr4.4:15076184-15076203 None:intergenic 50.0%
! TCATGCGGATTTTGCGGGTT+CGG + chr4.4:15075911-15075930 MS.gene08996:intron 50.0%
!! GCACCAAGCATTGACCTTCT+TGG + chr4.4:15075981-15076000 MS.gene08996:intron 50.0%
AAAGGAGGAAACGGTCGGTC+CGG - chr4.4:15075889-15075908 None:intergenic 55.0%
AGTCTCGTTGTACCCGGTAG+GGG - chr4.4:15075960-15075979 None:intergenic 55.0%
CTCTCACCTCACACTCCAAC+AGG - chr4.4:15075703-15075722 None:intergenic 55.0%
GAGTCTCGTTGTACCCGGTA+GGG - chr4.4:15075961-15075980 None:intergenic 55.0%
GTGGGTCCAATACAGCCATC+CGG + chr4.4:15075802-15075821 MS.gene08996:intron 55.0%
! CGGATTTTGCGGGTTCGGAT+CGG + chr4.4:15075916-15075935 MS.gene08996:intron 55.0%
! GGACGGGTTCGGATAACAGT+GGG + chr4.4:15075784-15075803 MS.gene08996:intron 55.0%
! GGATTTTGCGGGTTCGGATC+GGG + chr4.4:15075917-15075936 MS.gene08996:intron 55.0%
!! GAAGTGGTGGACGGTGATGA+CGG + chr4.4:15075612-15075631 MS.gene08996:CDS 55.0%
AAGGAGGAAACGGTCGGTCC+GGG - chr4.4:15075888-15075907 None:intergenic 60.0%
ACCCGGTAGGGGTGGACAAA+CGG - chr4.4:15075949-15075968 None:intergenic 60.0%
ACCCGTTTGTCCACCCCTAC+CGG + chr4.4:15075944-15075963 MS.gene08996:intron 60.0%
CGAGTCTCGTTGTACCCGGT+AGG - chr4.4:15075962-15075981 None:intergenic 60.0%
CTCTCTCTCTCATCGTCCGC+CGG - chr4.4:15075640-15075659 None:intergenic 60.0%
GGTGCGAGTCTCGTTGTACC+CGG - chr4.4:15075966-15075985 None:intergenic 60.0%
TCTAACCCGCCAAACCGACC+CGG + chr4.4:15075867-15075886 MS.gene08996:intron 60.0%
TCTCTCTCTCATCGTCCGCC+GGG - chr4.4:15075639-15075658 None:intergenic 60.0%
TGGTTTAGTGTGGGGCGGTC+CGG - chr4.4:15075824-15075843 None:intergenic 60.0%
! CGGACGGGTTCGGATAACAG+TGG + chr4.4:15075783-15075802 MS.gene08996:intron 60.0%
!! GGTCGGTTTGGCGGGTTAGA+CGG - chr4.4:15075867-15075886 None:intergenic 60.0%
!! GGTTTGGCGGGTTAGACGGA+TGG - chr4.4:15075863-15075882 None:intergenic 60.0%
AGGAAACGGTCGGTCCGGGT+CGG - chr4.4:15075884-15075903 None:intergenic 65.0%
CCCGGTAGGGGTGGACAAAC+GGG - chr4.4:15075948-15075967 None:intergenic 65.0%
CCCGTTTGTCCACCCCTACC+GGG + chr4.4:15075945-15075964 MS.gene08996:intron 65.0%
CTCGTTGTACCCGGTAGGGG+TGG - chr4.4:15075957-15075976 None:intergenic 65.0%
GGCGGTCCGGATGGCTGTAT+TGG - chr4.4:15075811-15075830 None:intergenic 65.0%
TTAGTGTGGGGCGGTCCGGA+TGG - chr4.4:15075820-15075839 None:intergenic 65.0%
!! GTGGACGGTGATGACGGTCC+CGG + chr4.4:15075618-15075637 MS.gene08996:CDS 65.0%
ACGGTCGGTCCGGGTCGGTT+TGG - chr4.4:15075879-15075898 None:intergenic 70.0%
!! GACGGTGATGACGGTCCCGG+CGG + chr4.4:15075621-15075640 MS.gene08996:CDS 70.0%
!! GTCGGTCCGGGTCGGTTTGG+CGG - chr4.4:15075876-15075895 None:intergenic 70.0%
!! TCGGTCCGGGTCGGTTTGGC+GGG - chr4.4:15075875-15075894 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 15075586 15077329 15075586 ID=MS.gene08996
chr4.4 mRNA 15075586 15077329 15075586 ID=MS.gene08996.t1;Parent=MS.gene08996
chr4.4 exon 15075586 15075666 15075586 ID=MS.gene08996.t1.exon1;Parent=MS.gene08996.t1
chr4.4 CDS 15075586 15075666 15075586 ID=cds.MS.gene08996.t1;Parent=MS.gene08996.t1
chr4.4 exon 15076559 15077329 15076559 ID=MS.gene08996.t1.exon2;Parent=MS.gene08996.t1
chr4.4 CDS 15076559 15077329 15076559 ID=cds.MS.gene08996.t1;Parent=MS.gene08996.t1
Gene Sequence

>MS.gene08996

ATGTTGGATGGAGTTTATAAGAGAAGGAAGTGGTGGACGGTGATGACGGTCCCGGCGGACGATGAGAGAGAGAGAACTATGGTGAGTGAGGATGAGATACCAGTGTTGTTGTTGCCTGTTGGAGTGTGAGGTGAGAGACAAAGGCAAGAGACAAAAACGACGTCTGTGATAAAAATAATTAAATACATAATATTATGCGGACGGGTTCGGATAACAGTGGGTCCAATACAGCCATCCGGACCGCCCCACACTAAACCAGTAATATCCGATTTGACCATCCGTCTAACCCGCCAAACCGACCCGGACCGACCGTTTCCTCCTTTTTTCATGCGGATTTTGCGGGTTCGGATCGGGTGAGACCCGTTTGTCCACCCCTACCGGGTACAACGAGACTCGCACCAAGCATTGACCTTCTTGGTATTCTCCCTCATCCTGGTTTCTCCTTCTGCTTTTGATTCGAAATCCAGAATCTATGTTTGTTCTATTCTAATATCTATATCTTCTTTTTCCTGCTTTCAAACCTAAAATAAAATCCTTTATTTTCATGTCTTATTCTTATTATTCATGCCCGCAACATGTTTGTTAAATTGAAAAGCCTAGTCCTACCAATGCAATCCCTCAAGTTGTTACATTCTCTTCCTTTCAGTTCTAACTCACTTCGTTCTTTTCTATGTTCATTTTCTTCTTTATCAACCCCAATTAACCAATCTTCAAACCAAGAAACCACTTTTCTGTTTAACCTCCTTAATTCCAATTCCAAATTACATAAATCTGAATCCATCTATGTATCTAAACTTGTTTCAGGAATCACTTCCCCTGAAAGCCCTTATCTGTAATCAACTTTTTCAAACAAATTGGATTTTCTCAAACTCAGATACACTCCATCCTTTGTCAGAGAACTCAAATACTCTTTTCAAATGTTCATAAAACCCTTAAACCTAAAGTTGAATTCTTTCAACAATTGGGTTTTCAGGGTTCTCAATTAGGTCTTTTTCTTTCTAAGAATCCAACCATTTTGTCTGCTAGTTTGAACAAAACATTGGTCCCATCTGTGGAAGCTATTAAGAAAATTGTAGGGAATGAGAAAGATCTTATTCAAGTTTTGAGCTACTGTGGCTGGGTATTACCAGTGTACCAAACATTTGTTGCCAACATTGCTTTCTTAGAGAGTTGTGGCATTGTTGGGGATCAGGTTTTGATTTTAATCAAACATTCAAGACTTTTGACTGCACCACAATCTCGTATTAGAAACTATGTTTTGCAAGCTGAAAAATTGGGTTTCCATCAAAATTCAAGAATGCTTGTTCATGCGCTTCATACATTATGTTGTTTGAGCCATAAAACATTTAGGAAAAAGCTTGATTTCATTCAGTGTTTTGGGTTTTCTAAAGATGAGAGCTTGCAAATGTTCAAAAAGGTTCCAAATTTGCTTAGGGTATCAGAGAAGAAGTTAAAGGTTGGAATTGAGTTCTTCTTAGATACAGTTATGCTGCCAAAGTCAGCATTGGTTAATCGGCCTCTGATTTTGATGTATAGCATGGAGGATCGAGTGTTTCCCCGATTTAGAGTTTTTCAGCTTTTGAAATCACAAAATCTATGTAAGAAGGTTCCAAGCTTTCTTTATGTGTTAAATTTATCTGAGGACACATTCTTGGACAAGTTTATACCAAAGTTTAAAGAAAATGCAGAGGCATTATTGATAGCTTACAAAGGGGCGTTGTCTATAACCGTAATGATCTCTTGA
Protein sequence

>MS.gene08996.t1

MLDGVYKRRKWWTVMTVPADDERERTMGSQLGLFLSKNPTILSASLNKTLVPSVEAIKKIVGNEKDLIQVLSYCGWVLPVYQTFVANIAFLESCGIVGDQVLILIKHSRLLTAPQSRIRNYVLQAEKLGFHQNSRMLVHALHTLCCLSHKTFRKKLDFIQCFGFSKDESLQMFKKVPNLLRVSEKKLKVGIEFFLDTVMLPKSALVNRPLILMYSMEDRVFPRFRVFQLLKSQNLCKKVPSFLYVLNLSEDTFLDKFIPKFKENAEALLIAYKGALSITVMIS