AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene09027

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09027.t1 MTR_8g080680 84.461 399 34 2 349 721 1 397 0.0 676
MS.gene09027.t1 MTR_8g080680 62.169 378 85 7 1 341 1 357 1.23e-141 421
MS.gene09027.t1 MTR_8g080690 84.267 375 25 2 1 341 1 375 0.0 574
MS.gene09027.t1 MTR_8g080690 62.730 381 90 6 349 682 1 376 1.03e-147 436
MS.gene09027.t1 MTR_8g080670 58.645 428 87 4 349 704 1 410 6.69e-148 437
MS.gene09027.t1 MTR_8g080670 55.314 414 107 8 1 352 1 398 8.61e-115 352
MS.gene09027.t1 MTR_3g085180 44.521 438 171 7 349 724 1 428 1.93e-99 313
MS.gene09027.t1 MTR_3g085180 45.812 382 153 8 1 332 1 378 1.27e-84 275
MS.gene09027.t1 MTR_1g053715 33.762 311 156 8 428 707 18 309 3.05e-34 134
MS.gene09027.t1 MTR_1g053715 37.549 253 120 7 105 322 25 274 6.50e-34 133
MS.gene09027.t1 MTR_7g092930 36.364 242 114 8 144 352 61 295 5.54e-33 130
MS.gene09027.t1 MTR_7g092930 36.820 239 132 6 433 654 17 253 4.48e-32 127
MS.gene09027.t1 MTR_3g099080 52.252 111 52 1 151 261 98 207 1.54e-30 124
MS.gene09027.t1 MTR_3g099080 45.113 133 71 1 461 593 77 207 2.20e-28 118
MS.gene09027.t1 MTR_8g463140 66.250 80 27 0 149 228 63 142 2.22e-30 118
MS.gene09027.t1 MTR_8g463140 61.842 76 29 0 479 554 62 137 1.13e-27 110
MS.gene09027.t1 MTR_8g096780 48.031 127 64 1 137 263 85 209 5.45e-29 120
MS.gene09027.t1 MTR_8g096780 46.491 114 58 1 482 595 99 209 1.04e-23 104
MS.gene09027.t1 MTR_2g078770 65.789 76 26 0 149 224 56 131 5.77e-29 114
MS.gene09027.t1 MTR_2g078770 58.667 75 31 0 480 554 56 130 4.83e-25 102
MS.gene09027.t1 MTR_7g444860 46.405 153 70 4 99 242 98 247 1.74e-28 117
MS.gene09027.t1 MTR_7g444860 42.857 147 72 3 408 554 87 221 6.21e-28 115
MS.gene09027.t1 MTR_4g109770 40.000 185 85 6 151 328 123 288 1.09e-27 117
MS.gene09027.t1 MTR_4g109770 55.814 86 36 1 482 567 123 206 4.47e-25 109
MS.gene09027.t1 MTR_4g088555 65.789 76 26 0 479 554 60 135 1.28e-27 109
MS.gene09027.t1 MTR_4g088555 63.158 76 28 0 149 224 61 136 2.15e-26 105
MS.gene09027.t1 MTR_5g046670 56.977 86 37 0 149 234 242 327 1.23e-26 114
MS.gene09027.t1 MTR_5g046670 41.600 125 72 1 430 554 193 316 9.28e-26 112
MS.gene09027.t1 MTR_7g028740 58.750 80 33 0 151 230 77 156 1.88e-26 112
MS.gene09027.t1 MTR_7g028740 56.164 73 32 0 482 554 77 149 7.68e-23 101
MS.gene09027.t1 MTR_8g005960 64.103 78 28 0 149 226 185 262 2.19e-26 111
MS.gene09027.t1 MTR_8g005960 48.387 124 53 3 431 554 147 259 1.28e-25 108
MS.gene09027.t1 MTR_2g461920 55.556 90 40 0 137 226 157 246 3.37e-26 114
MS.gene09027.t1 MTR_2g461920 60.274 73 29 0 482 554 171 243 1.15e-23 106
MS.gene09027.t1 MTR_1g035010 57.831 83 35 0 148 230 101 183 3.82e-25 112
MS.gene09027.t1 MTR_1g035010 46.602 103 53 1 454 554 74 176 1.34e-21 100
MS.gene09027.t1 MTR_2g014200 56.250 80 35 0 149 228 62 141 9.79e-25 100
MS.gene09027.t1 MTR_2g014200 47.917 96 48 1 459 554 43 136 6.79e-24 98.6
MS.gene09027.t1 MTR_1g086250 48.718 117 55 1 119 230 110 226 3.20e-24 109
MS.gene09027.t1 MTR_1g086250 55.000 80 36 0 482 561 147 226 2.15e-21 100
MS.gene09027.t1 MTR_2g046550 58.974 78 32 0 151 228 139 216 4.76e-23 105
MS.gene09027.t1 MTR_2g046550 56.579 76 33 0 482 557 139 214 5.18e-21 99.0
MS.gene09027.t1 MTR_7g110320 55.294 85 38 0 151 235 145 229 2.81e-22 102
MS.gene09027.t1 MTR_7g110320 48.000 100 52 0 467 566 130 229 5.72e-21 98.6
MS.gene09027.t1 MTR_7g110320 55.294 85 38 0 151 235 145 229 3.69e-22 102
MS.gene09027.t1 MTR_7g110320 48.000 100 52 0 467 566 130 229 9.09e-21 98.2
MS.gene09027.t1 MTR_2g020620 39.496 119 58 2 158 269 97 208 2.06e-17 87.0
MS.gene09027.t1 MTR_2g020620 35.256 156 81 4 489 632 97 244 6.94e-16 82.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09027.t1 AT5G43270 47.561 246 109 8 1 227 1 245 2.51e-45 168
MS.gene09027.t1 AT5G43270 60.448 134 51 2 422 554 109 241 2.30e-39 151
MS.gene09027.t1 AT5G43270 47.561 246 109 8 1 227 1 245 2.51e-45 168
MS.gene09027.t1 AT5G43270 60.448 134 51 2 422 554 109 241 2.30e-39 151
MS.gene09027.t1 AT5G43270 47.561 246 109 8 1 227 1 245 2.51e-45 168
MS.gene09027.t1 AT5G43270 60.448 134 51 2 422 554 109 241 2.30e-39 151
MS.gene09027.t1 AT1G27360 43.825 251 115 8 1 226 1 250 1.44e-44 165
MS.gene09027.t1 AT1G27360 37.450 251 108 5 349 554 1 247 2.75e-38 147
MS.gene09027.t1 AT1G27360 43.825 251 115 8 1 226 1 250 1.44e-44 165
MS.gene09027.t1 AT1G27360 37.450 251 108 5 349 554 1 247 2.75e-38 147
MS.gene09027.t1 AT1G27360 43.825 251 115 8 1 226 1 250 1.44e-44 165
MS.gene09027.t1 AT1G27360 37.450 251 108 5 349 554 1 247 2.75e-38 147
MS.gene09027.t1 AT1G27360 43.825 251 115 8 1 226 1 250 1.44e-44 165
MS.gene09027.t1 AT1G27360 37.450 251 108 5 349 554 1 247 2.75e-38 147
MS.gene09027.t1 AT1G27370 41.353 266 129 6 1 240 1 265 8.37e-43 159
MS.gene09027.t1 AT1G27370 58.015 131 53 2 425 554 119 248 1.77e-40 152
MS.gene09027.t1 AT1G27370 41.353 266 129 6 1 240 1 265 3.73e-42 158
MS.gene09027.t1 AT1G27370 58.015 131 53 2 425 554 119 248 4.84e-40 152
MS.gene09027.t1 AT1G27370 41.353 266 129 6 1 240 1 265 3.73e-42 158
MS.gene09027.t1 AT1G27370 58.015 131 53 2 425 554 119 248 4.84e-40 152
MS.gene09027.t1 AT1G27370 41.353 266 129 6 1 240 1 265 3.73e-42 158
MS.gene09027.t1 AT1G27370 58.015 131 53 2 425 554 119 248 4.84e-40 152
MS.gene09027.t1 AT1G27370 41.353 266 129 6 1 240 1 265 3.73e-42 158
MS.gene09027.t1 AT1G27370 58.015 131 53 2 425 554 119 248 4.84e-40 152
MS.gene09027.t1 AT1G27370 41.353 266 129 6 1 240 1 265 3.73e-42 158
MS.gene09027.t1 AT1G27370 58.015 131 53 2 425 554 119 248 4.84e-40 152
MS.gene09027.t1 AT1G27370 41.353 266 129 6 1 240 1 265 3.73e-42 158
MS.gene09027.t1 AT1G27370 58.015 131 53 2 425 554 119 248 4.84e-40 152
MS.gene09027.t1 AT2G42200 54.955 111 45 2 148 258 71 176 5.81e-31 125
MS.gene09027.t1 AT2G42200 61.333 75 29 0 480 554 72 146 3.70e-26 111
MS.gene09027.t1 AT1G69170 64.103 78 28 0 147 224 120 197 7.06e-28 117
MS.gene09027.t1 AT1G69170 64.000 75 27 0 480 554 122 196 4.71e-27 114
MS.gene09027.t1 AT5G50670 57.143 84 36 0 147 230 97 180 5.58e-27 113
MS.gene09027.t1 AT5G50670 50.602 83 41 0 472 554 91 173 4.73e-22 99.0
MS.gene09027.t1 AT5G50670 57.143 84 36 0 147 230 97 180 5.58e-27 113
MS.gene09027.t1 AT5G50670 50.602 83 41 0 472 554 91 173 4.73e-22 99.0
MS.gene09027.t1 AT5G50570 57.143 84 36 0 147 230 97 180 5.58e-27 113
MS.gene09027.t1 AT5G50570 50.602 83 41 0 472 554 91 173 4.73e-22 99.0
MS.gene09027.t1 AT5G50570 57.143 84 36 0 147 230 97 180 5.58e-27 113
MS.gene09027.t1 AT5G50570 50.602 83 41 0 472 554 91 173 4.73e-22 99.0
MS.gene09027.t1 AT3G57920 30.508 295 165 11 433 710 27 298 1.18e-26 112
MS.gene09027.t1 AT3G57920 39.766 171 86 5 150 304 58 227 2.72e-25 108
MS.gene09027.t1 AT1G76580 55.914 93 41 0 148 240 111 203 1.38e-26 116
MS.gene09027.t1 AT1G76580 52.809 89 42 0 480 568 112 200 4.33e-22 102
MS.gene09027.t1 AT1G76580 55.914 93 41 0 148 240 111 203 1.48e-26 116
MS.gene09027.t1 AT1G76580 52.809 89 42 0 480 568 112 200 4.53e-22 102
MS.gene09027.t1 AT1G76580 55.914 93 41 0 148 240 111 203 2.67e-26 115
MS.gene09027.t1 AT1G76580 52.809 89 42 0 480 568 112 200 8.75e-22 101
MS.gene09027.t1 AT1G02065 63.415 82 29 1 146 226 183 264 2.79e-26 110
MS.gene09027.t1 AT1G02065 46.154 130 60 1 431 560 147 266 3.37e-26 110
MS.gene09027.t1 AT2G33810 60.811 74 29 0 151 224 54 127 1.60e-25 102
MS.gene09027.t1 AT2G33810 55.556 90 38 1 465 554 39 126 1.70e-25 102
MS.gene09027.t1 AT3G60030 55.814 86 38 0 151 236 127 212 5.37e-25 111
MS.gene09027.t1 AT3G60030 57.534 73 31 0 482 554 127 199 1.74e-21 100
MS.gene09027.t1 AT1G20980 53.465 101 46 1 137 236 106 206 8.28e-25 111
MS.gene09027.t1 AT1G20980 57.333 75 32 0 480 554 118 192 8.77e-21 98.6
MS.gene09027.t1 AT1G53160 56.962 79 34 0 151 229 54 132 6.29e-24 99.8
MS.gene09027.t1 AT1G53160 60.274 73 29 0 482 554 54 126 1.29e-23 98.6
MS.gene09027.t1 AT2G47070 61.538 78 30 0 151 228 106 183 6.51e-24 108
MS.gene09027.t1 AT2G47070 58.904 73 30 0 482 554 106 178 2.51e-21 100
MS.gene09027.t1 AT1G53160 56.962 79 34 0 151 229 54 132 8.03e-24 99.4
MS.gene09027.t1 AT1G53160 60.274 73 29 0 482 554 54 126 1.35e-23 99.0
MS.gene09027.t1 AT1G53160 56.962 79 34 0 151 229 54 132 8.03e-24 99.4
MS.gene09027.t1 AT1G53160 60.274 73 29 0 482 554 54 126 1.35e-23 99.0
MS.gene09027.t1 AT3G15270 56.757 74 32 0 151 224 63 136 6.76e-23 97.1
MS.gene09027.t1 AT3G15270 56.164 73 32 0 482 554 63 135 1.27e-22 96.3
MS.gene09027.t1 AT5G18830 53.333 75 35 0 150 224 137 211 2.63e-19 93.2
MS.gene09027.t1 AT5G18830 42.727 110 53 2 445 554 111 210 1.18e-17 88.2
MS.gene09027.t1 AT5G18830 53.333 75 35 0 150 224 137 211 2.71e-19 93.2
MS.gene09027.t1 AT5G18830 42.727 110 53 2 445 554 111 210 1.38e-17 87.8
MS.gene09027.t1 AT5G18830 53.333 75 35 0 150 224 137 211 2.88e-19 93.2
MS.gene09027.t1 AT5G18830 42.727 110 53 2 445 554 111 210 1.47e-17 87.8
MS.gene09027.t1 AT1G02065 43.434 99 46 2 431 529 147 235 8.31e-17 81.3
MS.gene09027.t1 AT1G02065 58.929 56 23 0 143 198 180 235 6.57e-16 78.6
MS.gene09027.t1 AT2G47070 60.377 53 21 0 176 228 2 54 9.03e-13 72.4

Find 124 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGTTTGGTGGTTGTAATTT+TGG 0.078496 4.4:-15380520 None:intergenic
CAGCTTGGTGGTTGTAATTT+TGG 0.089177 4.4:-15375761 None:intergenic
CATGATCTGTTTGTGTTTGA+TGG 0.199580 4.4:+15381165 MS.gene09027:CDS
CAAATAGTTCATCGTCAAAT+AGG 0.221746 4.4:+15375882 MS.gene09027:CDS
CCAAACCTTTCTTGTGTATC+TGG 0.244468 4.4:+15380646 MS.gene09027:CDS
CTTGACCGAGAAATCAATTT+CGG 0.268342 4.4:+15380553 MS.gene09027:CDS
TGACTTGATTGGAGTGCTTC+AGG 0.279073 4.4:-15376544 None:intergenic
TTCAAAGAACCTTATGAGTA+TGG 0.284972 4.4:+15382618 MS.gene09027:CDS
TCAATCTAAGACTTGGTAAA+AGG 0.294020 4.4:+15380680 MS.gene09027:CDS
CTCCCTCTTCCCAGTGGGAC+TGG 0.294118 4.4:+15375699 MS.gene09027:CDS
TCTGGCTCTGAGCTAATTCA+TGG 0.311663 4.4:+15380607 MS.gene09027:CDS
TATAACCACCATGGGAGATT+TGG 0.333181 4.4:-15380911 None:intergenic
CTTGCGTCATCTTTAAGTAT+TGG 0.335589 4.4:+15376064 MS.gene09027:CDS
TATAACCACCATAGGAGATT+TGG 0.339545 4.4:-15376209 None:intergenic
ATTAGAATGTTCTTCAGAAA+TGG 0.343450 4.4:-15382436 None:intergenic
ACAACCACCAAACTGGAGTA+TGG 0.348781 4.4:+15380528 MS.gene09027:CDS
CAAAATTACAACCACCAAAC+TGG 0.349918 4.4:+15380521 MS.gene09027:CDS
AGCTTTACCTGCCACATTGC+TGG 0.352012 4.4:-15376255 None:intergenic
GCAGTCTTGGAATTAGAAAA+TGG 0.356442 4.4:-15382081 None:intergenic
AGCTTTACCTGCTACATTGC+TGG 0.356873 4.4:-15380957 None:intergenic
TCTCCCATGGTGGTTATAGA+TGG 0.357918 4.4:+15380916 MS.gene09027:CDS
CATGGTGGTTATAGATGGTT+TGG 0.360015 4.4:+15380921 MS.gene09027:CDS
TTGACCGAGAAATCAATTTC+GGG 0.364920 4.4:+15380554 MS.gene09027:CDS
ACAAAACTGATTGACTTGAT+TGG 0.364968 4.4:-15376555 None:intergenic
ATCAGAAAAGCATAGAAATT+AGG 0.376805 4.4:-15380412 None:intergenic
ACAACCCGAAATTGATTTCT+CGG 0.376943 4.4:-15380558 None:intergenic
TCCCTCTTCCCAGTGGGACT+GGG 0.389604 4.4:+15375700 MS.gene09027:CDS
CTGATTCATTGCATGAGTTA+TGG 0.392149 4.4:-15382481 None:intergenic
ATAAAGCTGATCGGCTTGAT+TGG 0.396774 4.4:-15381260 None:intergenic
CCCGGTGGAAGTGGTTCTTC+TGG 0.397175 4.4:+15380589 MS.gene09027:CDS
CCAGAGCTTTCTTCTGTCTC+TGG 0.403530 4.4:+15375950 MS.gene09027:CDS
TCTCCTATGGTGGTTATAGA+TGG 0.407218 4.4:+15376214 MS.gene09027:CDS
ACAACCCGGCATTGATTTCT+CGG 0.409997 4.4:-15375799 None:intergenic
GGCTAAATTTGTAGCAGTCT+TGG 0.413824 4.4:-15382094 None:intergenic
CTTGCAATTTCCGCGCTGCC+AGG 0.416641 4.4:+15376114 MS.gene09027:CDS
AAATAGTTCATCGTCAAATA+GGG 0.430475 4.4:+15375883 MS.gene09027:CDS
TTCACAGTTGGAGTTGGAAT+GGG 0.437116 4.4:+15380459 MS.gene09027:CDS
CGCTGCCAGGTTGAAGGTTG+TGG 0.443635 4.4:+15376127 MS.gene09027:CDS
TTTCCGCGCTGCCAGGTTGA+AGG 0.450558 4.4:+15376121 MS.gene09027:CDS
CATCAAACACAAACAGATCA+TGG 0.453038 4.4:-15381164 None:intergenic
CAGCTTGACGATGATATATA+CGG 0.462985 4.4:-15381236 None:intergenic
CTTCACAGTTGGAGTTGGAA+TGG 0.465688 4.4:+15380458 MS.gene09027:CDS
ATTGGTGCTCCCAGTCCCAC+TGG 0.471654 4.4:-15375709 None:intergenic
CCACCACGCTGCCAGGTTGA+AGG 0.471978 4.4:+15380823 MS.gene09027:CDS
CGCATATGAATCCCACTGAT+TGG 0.473627 4.4:-15382406 None:intergenic
CTCCCAGTCCCACTGGGAAG+AGG 0.474118 4.4:-15375702 None:intergenic
TCCCTCTTCACAGTTGGAGT+TGG 0.476978 4.4:+15380453 MS.gene09027:CDS
CCAGATACACAAGAAAGGTT+TGG 0.481067 4.4:-15380646 None:intergenic
TGTTTGATGCTCCAGCTTGG+TGG 0.481479 4.4:-15375773 None:intergenic
CTCTTGCTTCATCAGAGCCA+TGG 0.481784 4.4:+15382511 MS.gene09027:CDS
TTTATCATTTGTATCAGAAA+CGG 0.482839 4.4:-15377760 None:intergenic
CAAAATTACAACCACCAAGC+TGG 0.484604 4.4:+15375762 MS.gene09027:CDS
TCAAGTTCCATACTCCAGTT+TGG 0.484691 4.4:-15380535 None:intergenic
TATGGTGGTTATAGATGGTA+TGG 0.485704 4.4:+15376219 MS.gene09027:CDS
AACATTCATGTTAACCCAGC+TGG 0.488020 4.4:-15382647 None:intergenic
CTCTTCTGTCAACTAATCCA+TGG 0.493619 4.4:+15377805 MS.gene09027:CDS
GCAGTCTCTGAATTAGCAAA+TGG 0.494415 4.4:-15377515 None:intergenic
TATGATACACCCGGTGGAAG+TGG 0.496488 4.4:+15380580 MS.gene09027:CDS
TGGTGAAAACCATACTCATA+AGG 0.504888 4.4:-15382627 None:intergenic
AGTTCCATACTCCAGTTTGG+TGG 0.506654 4.4:-15380532 None:intergenic
TCTTGTTTGATGCTCCAGCT+TGG 0.510678 4.4:-15375776 None:intergenic
ACAATGTCCACCACGCTGCC+AGG 0.516200 4.4:+15380816 MS.gene09027:CDS
GCCAGAAGAACCACTTCCAC+CGG 0.517418 4.4:-15380590 None:intergenic
ACATTGTAAGTTCTGACCAT+TGG 0.517425 4.4:-15380800 None:intergenic
AAGTCATTCCAAATCTCCTA+TGG 0.520495 4.4:+15376201 MS.gene09027:CDS
CTGACTCATTGCATGAGTTA+CGG 0.521872 4.4:-15377892 None:intergenic
AACTTTCACGTCACAATGCA+AGG 0.530580 4.4:+15376506 MS.gene09027:CDS
GATACACAAGAAAGGTTTGG+TGG 0.536044 4.4:-15380643 None:intergenic
GAACCATTGCTCACTCTACT+CGG 0.540591 4.4:+15375974 MS.gene09027:CDS
TCCAACTCCAACTGTGAAGA+GGG 0.543018 4.4:-15380454 None:intergenic
AACTTTCAAATCACAATGCA+AGG 0.545524 4.4:+15381211 MS.gene09027:CDS
CAAGACCGAGAAATCAATGC+CGG 0.550620 4.4:+15375794 MS.gene09027:CDS
TCCCAGTCCCACTGGGAAGA+GGG 0.552029 4.4:-15375701 None:intergenic
AGTTATGGCTTGAAAAGCAC+TGG 0.554669 4.4:-15382466 None:intergenic
CCATTGCTCAATCTAAGACT+TGG 0.554906 4.4:+15380673 MS.gene09027:CDS
GTTCACCAGATACACAAGAA+AGG 0.558590 4.4:-15380651 None:intergenic
TCTTCTGTCAACTAATCCAT+GGG 0.562513 4.4:+15377806 MS.gene09027:CDS
CATCGAACTCAAACAGATCG+TGG 0.568188 4.4:-15376459 None:intergenic
GATAATCTTTCGCTGATGAA+AGG 0.568732 4.4:-15376156 None:intergenic
CCAGAGACAGAAGAAAGCTC+TGG 0.576248 4.4:-15375950 None:intergenic
ATTAGAATGTTCTAGAGACA+TGG 0.583823 4.4:-15377847 None:intergenic
GAAAAGAAATGCAAGTCCAA+TGG 0.587403 4.4:+15380784 MS.gene09027:CDS
CCAAGTCTTAGATTGAGCAA+TGG 0.590002 4.4:-15380673 None:intergenic
AGTTACGGCTTGAACAGCAC+TGG 0.590790 4.4:-15377877 None:intergenic
CAAGAGCACAACCTTCAACC+TGG 0.591018 4.4:-15380834 None:intergenic
CAAGGCCACAACCTTCAACC+TGG 0.591428 4.4:-15376132 None:intergenic
AATATGTCAATTCCAACATG+TGG 0.594822 4.4:+15382550 MS.gene09027:CDS
CTCTTCTGTCAACCAATCAG+TGG 0.597609 4.4:+15382394 MS.gene09027:CDS
ACGGCTTGAACAGCACTGGT+TGG 0.597812 4.4:-15377873 None:intergenic
TTAGTCGCATATGAATCCCA+TGG 0.598308 4.4:-15377822 None:intergenic
TCTTCTGTCAACCAATCAGT+GGG 0.601400 4.4:+15382395 MS.gene09027:CDS
TTCCAACTCCAACTGTGAAG+AGG 0.601663 4.4:-15380455 None:intergenic
GCCGGGTTGTTCGATACACC+TGG 0.605439 4.4:+15375812 MS.gene09027:CDS
AATATCTCCCTCTTCACAGT+TGG 0.605827 4.4:+15380447 MS.gene09027:CDS
AAGACCGAGAAATCAATGCC+GGG 0.606643 4.4:+15375795 MS.gene09027:CDS
AGACTGCTACAAATTTAGCC+TGG 0.607461 4.4:+15377531 MS.gene09027:CDS
AGACTGCTACAAATTTAGCC+TGG 0.607461 4.4:+15382097 MS.gene09027:CDS
AAACCATCTATAACCACCAT+GGG 0.608088 4.4:-15380919 None:intergenic
CCAGAAGAACCACTTCCACC+GGG 0.608276 4.4:-15380589 None:intergenic
GCTTTATCTTATTCACGCAG+TGG 0.608852 4.4:+15381276 MS.gene09027:CDS
ATACCATCTATAACCACCAT+AGG 0.609763 4.4:-15376217 None:intergenic
GTGAATAAGATAAAGCTGAT+CGG 0.611733 4.4:-15381269 None:intergenic
CCTTCAACCTGGCAGCGTGG+TGG 0.616962 4.4:-15380823 None:intergenic
AGAGTTTGAGATCCACATGT+TGG 0.619666 4.4:-15382562 None:intergenic
TTCGGGTTGTATGATACACC+CGG 0.621273 4.4:+15380571 MS.gene09027:CDS
TTGGTGCTCCCAGTCCCACT+GGG 0.623897 4.4:-15375708 None:intergenic
TTTCCGAGTAGAGTGAGCAA+TGG 0.627280 4.4:-15375977 None:intergenic
CTTTCGCTGATGAAAGGTCA+AGG 0.632145 4.4:-15376150 None:intergenic
CAAACCATCTATAACCACCA+TGG 0.633030 4.4:-15380920 None:intergenic
TTAGCCTGGCAAAACATGAA+CGG 0.633733 4.4:+15377545 MS.gene09027:CDS
AATATCTCCCTCTTCCCAGT+GGG 0.634960 4.4:+15375694 MS.gene09027:CDS
CAATATCTCCCTCTTCCCAG+TGG 0.638747 4.4:+15375693 MS.gene09027:CDS
AAGTCATGCCAAATCTCCCA+TGG 0.638950 4.4:+15380903 MS.gene09027:CDS
CAACCTTCAACCTGGCAGCG+TGG 0.648763 4.4:-15380826 None:intergenic
TCATTCCAAATCTCCTATGG+TGG 0.656270 4.4:+15376204 MS.gene09027:CDS
CAACCTTCAACCTGGCAGCG+CGG 0.659706 4.4:-15376124 None:intergenic
CATGGCTCTGATGAAGCAAG+AGG 0.663007 4.4:-15382510 None:intergenic
CGGAAAACGAGTGTACATTG+AGG 0.675568 4.4:+15375994 MS.gene09027:CDS
GGGTTGTTCGATACACCTGG+TGG 0.681597 4.4:+15375815 MS.gene09027:CDS
AAAGACAACACTAACACACA+AGG 0.691256 4.4:+15377587 MS.gene09027:CDS
ACCAGGTGTATCGAACAACC+CGG 0.701562 4.4:-15375813 None:intergenic
TGATCATGATCACTACACCA+TGG 0.714032 4.4:-15382528 None:intergenic
TCATGCCAAATCTCCCATGG+TGG 0.722301 4.4:+15380906 MS.gene09027:CDS
GGGTTGTATGATACACCCGG+TGG 0.736684 4.4:+15380574 MS.gene09027:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr4.4 gene 15375680 15382659 15375680 ID=MS.gene09027
chr4.4 mRNA 15375680 15382659 15375680 ID=MS.gene09027.t1;Parent=MS.gene09027
chr4.4 exon 15375680 15376269 15375680 ID=MS.gene09027.t1.exon1;Parent=MS.gene09027.t1
chr4.4 CDS 15375680 15376269 15375680 ID=cds.MS.gene09027.t1;Parent=MS.gene09027.t1
chr4.4 exon 15376456 15376589 15376456 ID=MS.gene09027.t1.exon2;Parent=MS.gene09027.t1
chr4.4 CDS 15376456 15376589 15376456 ID=cds.MS.gene09027.t1;Parent=MS.gene09027.t1
chr4.4 exon 15377495 15377608 15377495 ID=MS.gene09027.t1.exon3;Parent=MS.gene09027.t1
chr4.4 CDS 15377495 15377608 15377495 ID=cds.MS.gene09027.t1;Parent=MS.gene09027.t1
chr4.4 exon 15377742 15377917 15377742 ID=MS.gene09027.t1.exon4;Parent=MS.gene09027.t1
chr4.4 CDS 15377742 15377917 15377742 ID=cds.MS.gene09027.t1;Parent=MS.gene09027.t1
chr4.4 exon 15380403 15380971 15380403 ID=MS.gene09027.t1.exon5;Parent=MS.gene09027.t1
chr4.4 CDS 15380403 15380971 15380403 ID=cds.MS.gene09027.t1;Parent=MS.gene09027.t1
chr4.4 exon 15381161 15381297 15381161 ID=MS.gene09027.t1.exon6;Parent=MS.gene09027.t1
chr4.4 CDS 15381161 15381297 15381161 ID=cds.MS.gene09027.t1;Parent=MS.gene09027.t1
chr4.4 exon 15382061 15382198 15382061 ID=MS.gene09027.t1.exon7;Parent=MS.gene09027.t1
chr4.4 CDS 15382061 15382198 15382061 ID=cds.MS.gene09027.t1;Parent=MS.gene09027.t1
chr4.4 exon 15382331 15382659 15382331 ID=MS.gene09027.t1.exon8;Parent=MS.gene09027.t1
chr4.4 CDS 15382331 15382659 15382331 ID=cds.MS.gene09027.t1;Parent=MS.gene09027.t1
Gene Sequence

>MS.gene09027

ATGGACTTGAATGCAATATCTCCCTCTTCCCAGTGGGACTGGGAGCACCAATCATTCTTCCACACAAAAGCAACTGAAAATCCAAAATTACAACCACCAAGCTGGAGCATCAAACAAGACCGAGAAATCAATGCCGGGTTGTTCGATACACCTGGTGGAAGTGATATTTTTGGCTCTGCATCAAGGAGTTCAAAATCAGCTTCAAATAGTTCATCGTCAAATAGGGATAATAGCAAAACATGTATTTTTGCTTGTGGAAATTCTCATACACCAGAGCTTTCTTCTGTCTCTGGTGAACCATTGCTCACTCTACTCGGAAAACGAGTGTACATTGAGGATGTATGTCCGAAAAGTAATTCCAAAAACCTATCTTTTTCAAGGGATCTTGCGTCATCTTTAAGTATTGGAAAGAAATGTAAGTTCAATAGTCAGAACTTGCAATTTCCGCGCTGCCAGGTTGAAGGTTGTGGCCTTGACCTTTCATCAGCGAAAGATTATCATCGCAAACGAAGAGTTTGTGAAAGTCATTCCAAATCTCCTATGGTGGTTATAGATGGTATGGAACGTCGATTTTGCCAGCAATGTGGCAGGTAAAGCTTCTTAACAAATGTATGATTGTTTCAATGTAATTGACATAGGAAATAATTTTTTTATTTTTTTTTTGGTAGTGACGAAATGTTGTTAGCACATCATTATAAGCTAATATCACTACTCAGTCTGATTAAACTTGCTGTCTTTTTTGAATGGTGTTGAATTATTTGCTTCGAATGCTGAAGGTTCCACGATCTGTTTGAGTTCGATGAAAAGAAAAGAAGCTGCAGAGAACAACTTTCACGTCACAATGCAAGGCGACGTAAGCATCAGCCTGAAGCACTCCAATCAAGTCAATCAGTTTTGTCTTCACGCTGTGGTAATACTTATTTTTCAATAGTTTTGCAGTTTATCTCAGTTTCTGCTTGTTATCTATGTTTGGGTGTTAAAGCTGAAAAGATGAAATGTGGAAACACAAGACCATGTTATGCATACCTTAGTTGACAATAAACATTGCTGAATGAAAGATGTTTTAAAACTTGACATGCTTAACGTCACATTTCTTTGCTGTCCTGCTTATTTGCGTAAGAGTTTACCTTGCAAGTTACAATGGACATTAAATGTGATGTGACAGAACTTCTTAATTTGCTACAATTTCCTATCTTTTTAAGCTCTTGTTAGTTCTGCTCATTTTTTCTTTAGTTTCATAGATCTTATTGGATTGTCAACTGTCATACGTTTCGATGGTAAAGAGGCTTAATCTTCTGTAACATGAATAAGTTAAGCATGTTCAAGAGTTCTAGCTGTAACAATTTTATAATAACCATGCAAAGTAACAGTCATGTTATTCTTGATAATTACTTATTGTCAGAAAGGATATTGCTATATTAGAGTTGTGGGATGAGGACTGGATATAGTTAAAGGCAGATTTAAGAGCCTAAGTCTTTAGAGAATTAAGGTAGCGTACCCCTTATTAAGGCATATGATACAAATAATTATCATGATTGTTTGGACTTGAGTGTGATTATGACATGCCTAAGCTGAAGTCTTTACTAACTAGATTTGTATGAGTATGCCATTCTTTAAAACCTGATTTTGTAAAAAAAACAACTTTAAATTTTGCTGTTAGTTGAAACATAGACCAAAGAACATTCACTTTTAGTTTTTTATAGGTTTATTGTAGTTTTTTTATAGGTTTATTGTAGTTAATTGCAAGTCGTTTATTTCTCAATGATTCAAATTTTACTGCTCTCTTCTAACTTCTAATATATTTTGGTTCCGCAGATGGAAAGCAACAGATGAGTCCATTTGCTAATTCAGAGACTGCTACAAATTTAGCCTGGCAAAACATGAACGGCCGCAAGCTCTTCCAAACAAAAGACAACACTAACACACAAGGTACTGTCACTATGCTTTCTGATGATTCCGTTGTCCATTTTACATCCAAAGTTCTTGCAACCAAAAGTATCCAATTGAGGTTATTTCACGTTTATTGCCACATTTTTTGTGCTGATTTTTTATAACTTTGCAGGTGTAGAAGATTTCATTACCGTTTCTGATACAAATGATAAACAAAACTTTACTTGTGCTCTCTCTCTTCTGTCAACTAATCCATGGGATTCATATGCGACTAAATCCATGTCTCTAGAACATTCTAATCGTCCAACCAGTGCTGTTCAAGCCGTAACTCATGCAATGAGTCAGCGCGTGCCTCTTGTTTAATCAGAATCATGGTCAACATGTCAATTCCAGCATGTGGATCTCATAATGTGAAGAATTTCAACTGTTCAGAGAACCTTATTTTTCTGGTTTTAACTAGTTTGATTAACATAAATGTTCTTACTCCAAATAGTCTCAACCTAATAAATTTATAGGACCTAAATTTTTGTTGTAATGACTATTTTTATTATCTCTCTCTTCTCGTGGTATCATAGCGAGAAACACGAAACAATTCTGGGAATATTTTTACTTGTGATTTAGTTAACTCACTTAACTGCATTTTCAAAAAAGGGGAGCTTAATCTGATCATACTAAAAGTCATTTTATTTGTCATATGTCAAAGTGCAGTGGTAATTTTGAACATTTTAGAGCCCGTTTGGAATTATTTTCATATCATTTCTGTTTCCAGACGTTGGTAACCTTCAATAAAGGATTTAAAGGACATACATTGTCAGTGTAAAATAGTTTTACATTGATATCCAATGAAAACCTATTGTTTTGTTGGCCACCTCACGATTATAAGGATATTTTGAAATTAATTAGTAGTACAACATGGATAAATTGTAGTTTCTCATTGGATGTCGGAGTAGAAGCTCATTGAACTCTTTTTATAATTCCAACTGAAAATAAAAAATGTTTTCTGAATCCAAAATAACCCTTGTCATCTGTTATTTATATAATCTAATATCTGTCCTGTTCTAATGCCAATTGTAGCCCCTTTTCAACTTTCTTCTTCTTCTTCTTCTTTTCTTTTCCAGTTGCCATACCCTTAGTTCGCTGCATTTTTATATTCCTTTGCTTACTTTTCTAAAACCTAAAGAACAAATCTATATACATATTCTAAAACTATATATATTTTGACAAATCTATATTATTTAGTAACAACAATATGGAAAAACAAAAGTAACCCATGCAAACAAAATAGATATGTTTTGATCTCAATATAGTTTATGATAGAACATTATAGTGTAATTCGATCCATGTAGCCGACCAGGGCCGTCTTAACAAATTCATAGGCCCGGTTTTAGTTTTATAAGGGATGCAAAAAAAATTAAATTATGGGCCCAAAAAAATTAAATAAGTCATTTTAAAAAAAAAAATATTATTTACACTTTGGTGGGTGGGATTTGAACCCATTACCTCTCACTCAACAAAAACTATCCTAGCCACTGAAGCACGCTAAACATATTGAAATTATTGGCTTTTAATAGATATATAACCATTATTTAGTGTCTTACATATTTTAAAAAAAAAATTTGGTAGCCCAGCCCCGACGGGCTATGGGCCGGCCCTGTAGCCGACCAACGGTCAACTTAATAACTAATTTTTTAAAAATAATCAAATCGTCTCATCTCAATTTCTTCAACGTTTATCAAATGAGTCCTTCCGAATTCGATATGTTAATTAACGTGCAATGTTGAACATTGAACTAGCCTACTTAAGGACGAAGTAGCTTACTGAGGGTAGTAAAGAAGAAGGCTGGATGGTTTTGTCTTTTCTGGTGGGCCACCTGTCCACTGTGCTTCTCTGCTCATTCCAAATGGTTGGAACACTGCTTTGTCCATTCAAACAACAAACAACGTTATAGCTAGCTAGCTGTTTGTCGCTTCTGCCACCACCTGTCACTATTCAAAACATCATTTTTATAACAAACAAACAATGCTCCCTCACTCCCACTCTTCCCTCACCCTTCCATTCTAGTGGTACGTTCCTCTTTCTCTGTTTTTCCTTTATCCTCTCTTCTTTTTTGAATTTTATATTTGCAGTTAAAATAAAAATAAACAACTTTAACTGTTAAGAATACCACTCTATTAATTACTATTTATGGTAAACTCATGTTCCTATTTTCTCTTTTTCAAAGACTTTGATAATAATAGTTTAAGTAAAGACTGGACTTTTATGAAAAAGAAGTGATTTTGTTGTGTTTGTCTTCTTTTTTTGGCTTATCATTTTCTGAGATAGTTAAGTTTTCATCAGTTTTTTTATCTATATCTCTATGCTGTAACTATGTAATTGTGTCTTTGATTCAGTAACAGCCAGTAACTGTTTAGTTGATGTTGTGTTTTATGATATTAATCCATTTTTTCATTTGCAATTAGAAATAGTGTTGATTTTTTTTATGCCTCTATGCTGCAATTATGCATTTGATTCAATAACAATCACTAACTGTTGAGTAGAAGTTGTTTTTGTATGATTAATCCATTTTTCTTGCCTTGGCAAATATTAATGGTGTTGATTATTTTAAAAAAAATGAAAAGGTTCACACTTTTTAGATGTTTTTTATGATTTCTCTACTGTGTACATATATGTATATTCTTTGTGCATTTTGTGAATCATTGATGCAGTTTATAAGCAGTTTCATTTCTTTAACTTGTTAAAGTGTCTATAAAACCCTGTTATACTTTTCCTTGTTTCTAGATTAGAAAAGCTACAACATTAGCATTGATAATCTCATTTGGTTTATACTTTGCAGATTAGATTACCTAATTTCTATGCTTTTCTGATGGACTTGAATGCAATATCTCCCTCTTCACAGTTGGAGTTGGAATGGGATCACCTACCATTACTTAATACAAAAGCAACTGAAAACCCAAAATTACAACCACCAAACTGGAGTATGGAACTTGACCGAGAAATCAATTTCGGGTTGTATGATACACCCGGTGGAAGTGGTTCTTCTGGCTCTGAGCTAATTCATGGTTCAGCATTTAATCCACCAAACCTTTCTTGTGTATCTGGTGAACCATTGCTCAATCTAAGACTTGGTAAAAGGATGTATTTTGAGGAAGTTTGTCCAGAAAGTGATTCCAAAAATCTTTCTTTTTCAAGGGATCTCATGTCATCTTCAATTCTTGAAAAGAAATGCAAGTCCAATGGTCAGAACTTACAATGTCCACCACGCTGCCAGGTTGAAGGTTGTGCTCTTGATCTTTCATCCGCAAAAGATTATCATCGCAAACGAAGAGTTTGTGAAAGTCATGCCAAATCTCCCATGGTGGTTATAGATGGTTTGGAGCGTCGATTTTGCCAGCAATGTAGCAGGTAAAGCTTCTTAACAACTGTATAATTGTTTAGATCTAATTGACATAGAAAATAATAGTATTTTTTTCGAATGATGAATTCTTGTTAGCACATCATTAAAAGCTAATATTACTACTCAATCTAATTCAACTTGCTGTTGGTGTTGAATAAATTGCTCTAGAATTGATCACTTTTCTTAAAATGCTGAAGGTTCCATGATCTGTTTGTGTTTGATGGAAAGAAGAAAAGCTGCAGACGACAACTTTCAAATCACAATGCAAGGCGCCGTATATATCATCGTCAAGCTGTCCAATCAAGCCGATCAGCTTTATCTTATTCACGCAGTGGTAATACTTATTTTTCTCTGATTTTGCAGTTTATCTCAGTTCATGCTTGTTATCTGTTTTTGATTGTTAAAGCTGAAAAAATGAAATGTAGAAATCTAAGAGGCTAAGACCATGTTATGCATGCCTTAGTTGACTATTAACATTGCTGAATGAAAACTGTTTGAAAAATTGAAATGCTTAACACCACTCTTCTTTGCTGTCCTGCTTATTTACTCAAGAGTTTGCCTTGCAAGTTACACGAGACATTAAATGTAATGCAACAGAACTTCTTAATTTTCTACAATTGCCTATCTTTTAACCTCTTGTAAGTTCTGTTCATTTTTCATTTATTTCATAGATCTTATTGGATTTTCAATCGTCATACATCTGGATGGTAAACATGATTAAACTTTTGAGTTGTGGACTAGATAGTTGATTTAGTTAAAGGCACAAGACATGGTTAAGATCCTAATTGGGGTAGCATACTCTTGATTACAGAATTACACAAATCATTATCCTGATTGTTAAGAGTTTGGACATGAGCGCGAGTATAACATTCCTAAGCCGAAGTATTTACTAACTAGATTTGTATGAGTATGCCATTCGTTAATACCTGGTTTAGTAAAAATACAACTTCAAATTGCTGTTAGTCAAAACTTAGACCAAAGAACATCCACTTTTGGTTGTTTTATAGGTTTATTATAGTTAATTGCAAGTCATTCATTTCTCAATGATTCAAATTTTACTGTTGTCTTCTATCTTCTAATATATTTTGGTTCTGCAGATGAGAAGCAACAGATGAGTCCATTTTCTAATTCCAAGACTGCTACAAATTTAGCCTGGCAAAACATGCACAACAGCAAGCTCCCCCAAGCAAAAGATTTTCCCTTGAAGCCTGCAAAAGACAACATTGACACACCAAGTACTGTCAATATGCTTTCTCATGATTCCAATTTCCATTTTACATCCAAAGTCCTTCCAACCAAGAGTATCAATCCAGGTTATTTCACGTTTATTGCCACATTTTTGTGCTGATTTTTTTTTACTCTTGCAGGTGTAGAAGATTTCATGAACTTTTCTGATACAAATGCTACACAAGACTTTACTTGTGCTCTCTCTCTTCTGTCAACCAATCAGTGGGATTCATATGCGACTAAATCCATTTCTGAAGAACATTCTAATCGCTCGACCAGTGCTTTTCAAGCCATAACTCATGCAATGAATCAGCGCATTCCTCTTGCTTCATCAGAGCCATGGTGTAGTGATCATGATCAATATGTCAATTCCAACATGTGGATCTCAAACTCTGCAGATAGCAATCACTTTCAGAAATTTCAACTGTTCAAAGAACCTTATGAGTATGGTTTTCACCAGCTGGGTTAA
Protein sequence

>MS.gene09027.t1

MDLNAISPSSQWDWEHQSFFHTKATENPKLQPPSWSIKQDREINAGLFDTPGGSDIFGSASRSSKSASNSSSSNRDNSKTCIFACGNSHTPELSSVSGEPLLTLLGKRVYIEDVCPKSNSKNLSFSRDLASSLSIGKKCKFNSQNLQFPRCQVEGCGLDLSSAKDYHRKRRVCESHSKSPMVVIDGMERRFCQQCGRFHDLFEFDEKKRSCREQLSRHNARRRKHQPEALQSSQSVLSSRCDGKQQMSPFANSETATNLAWQNMNGRKLFQTKDNTNTQGVEDFITVSDTNDKQNFTCALSLLSTNPWDSYATKSMSLEHSNRPTSAVQAVTHAMSQRIRLPNFYAFLMDLNAISPSSQLELEWDHLPLLNTKATENPKLQPPNWSMELDREINFGLYDTPGGSGSSGSELIHGSAFNPPNLSCVSGEPLLNLRLGKRMYFEEVCPESDSKNLSFSRDLMSSSILEKKCKSNGQNLQCPPRCQVEGCALDLSSAKDYHRKRRVCESHAKSPMVVIDGLERRFCQQCSRFHDLFVFDGKKKSCRRQLSNHNARRRIYHRQAVQSSRSALSYSRSDEKQQMSPFSNSKTATNLAWQNMHNSKLPQAKDFPLKPAKDNIDTPSVEDFMNFSDTNATQDFTCALSLLSTNQWDSYATKSISEEHSNRSTSAFQAITHAMNQRIPLASSEPWCSDHDQYVNSNMWISNSADSNHFQKFQLFKEPYEYGFHQLG