Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09106.t1 | RHN49639.1 | 66.7 | 75 | 24 | 1 | 15 | 89 | 94 | 167 | 4.10E-18 | 100.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09106.t1 | A0A072U4K3 | 66.7 | 75 | 24 | 1 | 15 | 89 | 99 | 172 | 3.0e-18 | 100.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene09106.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09106.t1 | MTR_7g117590 | 65.789 | 76 | 25 | 1 | 15 | 90 | 99 | 173 | 4.44e-28 | 100 |
| MS.gene09106.t1 | MTR_2g012700 | 38.636 | 88 | 54 | 0 | 1 | 88 | 32 | 119 | 3.72e-24 | 88.6 |
| MS.gene09106.t1 | MTR_2g062330 | 39.773 | 88 | 52 | 1 | 1 | 88 | 52 | 138 | 1.11e-21 | 82.8 |
| MS.gene09106.t1 | MTR_1g054470 | 58.182 | 55 | 22 | 1 | 24 | 78 | 80 | 133 | 2.25e-13 | 61.6 |
| MS.gene09106.t1 | MTR_0010s0310 | 36.264 | 91 | 53 | 2 | 1 | 86 | 160 | 250 | 5.21e-13 | 62.4 |
| MS.gene09106.t1 | MTR_6g087720 | 37.838 | 74 | 46 | 0 | 1 | 74 | 223 | 296 | 2.14e-12 | 61.2 |
| MS.gene09106.t1 | MTR_7g117585 | 34.940 | 83 | 54 | 0 | 7 | 89 | 64 | 146 | 4.75e-12 | 58.2 |
| MS.gene09106.t1 | MTR_3g008190 | 29.070 | 86 | 61 | 0 | 3 | 88 | 16 | 101 | 1.11e-11 | 56.2 |
| MS.gene09106.t1 | MTR_5g071410 | 34.940 | 83 | 54 | 0 | 7 | 89 | 187 | 269 | 1.87e-11 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 15 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGTAGTGCTGTTAGTAAT+TGG | 0.308508 | 4.1:-40588141 | MS.gene09106:CDS |
| GGCTGATGGGAGGAATGTTT+TGG | 0.371733 | 4.1:-40588367 | MS.gene09106:intron |
| AACTCTATGTATCTATGATT+AGG | 0.426823 | 4.1:-40588018 | MS.gene09106:CDS |
| GATTGGGTTGATATGGCTGA+TGG | 0.450527 | 4.1:-40588381 | None:intergenic |
| ATTGGGTTGATATGGCTGAT+GGG | 0.489099 | 4.1:-40588380 | None:intergenic |
| CTTCATTATTGACAATCCTC+CGG | 0.491140 | 4.1:-40588044 | MS.gene09106:CDS |
| ATAGATACATAGAGTTTGTC+CGG | 0.500986 | 4.1:+40588025 | None:intergenic |
| TAGTGCTGTTAGTAATTGGA+AGG | 0.528143 | 4.1:-40588137 | MS.gene09106:CDS |
| CGGGCAAAGGATCTAAAAGC+TGG | 0.556966 | 4.1:-40588079 | MS.gene09106:CDS |
| AGAAATATGTGATCAAAGGA+TGG | 0.560696 | 4.1:-40588114 | MS.gene09106:CDS |
| AAGGAGAAATATGTGATCAA+AGG | 0.567643 | 4.1:-40588118 | MS.gene09106:CDS |
| GGGTTGATATGGCTGATGGG+AGG | 0.648388 | 4.1:-40588377 | None:intergenic |
| CTTTGAGCAGCAATATCATG+AGG | 0.656330 | 4.1:+40588232 | None:intergenic |
| ACTTGTTGATGATGAAACTG+AGG | 0.677988 | 4.1:-40588182 | MS.gene09106:CDS |
| GATACATAGAGTTTGTCCGG+AGG | 0.726473 | 4.1:+40588028 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AACTCTATGTATCTATGATT+AGG | - | chr4.1:40588363-40588382 | MS.gene09106:intron | 25.0% |
| AAGGAGAAATATGTGATCAA+AGG | - | chr4.1:40588263-40588282 | MS.gene09106:intron | 30.0% | |
| AGAAATATGTGATCAAAGGA+TGG | - | chr4.1:40588267-40588286 | MS.gene09106:intron | 30.0% | |
| ATAGATACATAGAGTTTGTC+CGG | + | chr4.1:40588359-40588378 | None:intergenic | 30.0% | |
| ! | TTTGTAGTGCTGTTAGTAAT+TGG | - | chr4.1:40588240-40588259 | MS.gene09106:CDS | 30.0% |
| !! | TTTGTGAGTTCTGAATATTC+AGG | - | chr4.1:40588121-40588140 | MS.gene09106:CDS | 30.0% |
| CTATCATCCTCACAACTAAT+CGG | + | chr4.1:40588079-40588098 | None:intergenic | 35.0% | |
| CTTCATTATTGACAATCCTC+CGG | - | chr4.1:40588337-40588356 | MS.gene09106:intron | 35.0% | |
| ! | AAGGATGGCATGATTTTGTT+CGG | - | chr4.1:40588282-40588301 | MS.gene09106:intron | 35.0% |
| ! | TAGTGCTGTTAGTAATTGGA+AGG | - | chr4.1:40588244-40588263 | MS.gene09106:intron | 35.0% |
| ! | TTTAACACCGATTAGTTGTG+AGG | - | chr4.1:40588069-40588088 | MS.gene09106:CDS | 35.0% |
| !! | ACTTGTTGATGATGAAACTG+AGG | - | chr4.1:40588199-40588218 | MS.gene09106:CDS | 35.0% |
| CTTTGAGCAGCAATATCATG+AGG | + | chr4.1:40588152-40588171 | None:intergenic | 40.0% | |
| ! | AGGATGGCATGATTTTGTTC+GGG | - | chr4.1:40588283-40588302 | MS.gene09106:intron | 40.0% |
| GATACATAGAGTTTGTCCGG+AGG | + | chr4.1:40588356-40588375 | None:intergenic | 45.0% | |
| ! | GCATGATTTTGTTCGGGCAA+AGG | - | chr4.1:40588289-40588308 | MS.gene09106:intron | 45.0% |
| CGGGCAAAGGATCTAAAAGC+TGG | - | chr4.1:40588302-40588321 | MS.gene09106:intron | 50.0% | |
| ! | GGCTGATGGGAGGAATGTTT+TGG | - | chr4.1:40588014-40588033 | MS.gene09106:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 40588012 | 40588391 | 40588012 | ID=MS.gene09106 |
| chr4.1 | mRNA | 40588012 | 40588391 | 40588012 | ID=MS.gene09106.t1;Parent=MS.gene09106 |
| chr4.1 | exon | 40588368 | 40588391 | 40588368 | ID=MS.gene09106.t1.exon1;Parent=MS.gene09106.t1 |
| chr4.1 | CDS | 40588368 | 40588391 | 40588368 | ID=cds.MS.gene09106.t1;Parent=MS.gene09106.t1 |
| chr4.1 | exon | 40588012 | 40588260 | 40588012 | ID=MS.gene09106.t1.exon2;Parent=MS.gene09106.t1 |
| chr4.1 | CDS | 40588012 | 40588260 | 40588012 | ID=cds.MS.gene09106.t1;Parent=MS.gene09106.t1 |
| Gene Sequence |
| Protein sequence |
