AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene09219

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09219.t1 MTR_8g080680 91.136 361 32 0 1 361 37 397 0.0 678
MS.gene09219.t1 MTR_8g080690 67.638 343 87 4 1 322 37 376 1.93e-152 434
MS.gene09219.t1 MTR_8g080670 61.795 390 87 2 1 344 37 410 1.64e-150 431
MS.gene09219.t1 MTR_3g085180 47.643 403 162 7 1 364 36 428 6.61e-103 310
MS.gene09219.t1 MTR_1g053715 33.127 323 168 10 42 347 18 309 4.89e-35 132
MS.gene09219.t1 MTR_7g092930 35.798 257 136 6 47 294 17 253 2.85e-34 129
MS.gene09219.t1 MTR_7g444860 43.537 147 71 3 22 168 87 221 3.69e-30 117
MS.gene09219.t1 MTR_3g099080 44.361 133 72 1 75 207 77 207 9.83e-30 118
MS.gene09219.t1 MTR_8g463140 63.158 76 28 0 93 168 62 137 1.26e-29 112
MS.gene09219.t1 MTR_4g088555 67.105 76 25 0 93 168 60 135 1.56e-29 111
MS.gene09219.t1 MTR_5g046670 42.400 125 71 1 44 168 193 316 5.18e-28 115
MS.gene09219.t1 MTR_8g005960 49.194 124 52 3 45 168 147 259 8.45e-28 111
MS.gene09219.t1 MTR_4g109770 56.977 86 35 1 96 181 123 206 2.93e-27 112
MS.gene09219.t1 MTR_2g078770 60.000 75 30 0 94 168 56 130 5.53e-27 105
MS.gene09219.t1 MTR_2g461920 61.644 73 28 0 96 168 171 243 9.07e-26 108
MS.gene09219.t1 MTR_8g096780 47.368 114 57 1 96 209 99 209 9.95e-26 107
MS.gene09219.t1 MTR_2g014200 57.895 76 32 0 93 168 61 136 1.31e-25 100
MS.gene09219.t1 MTR_7g028740 57.534 73 31 0 96 168 77 149 7.42e-25 104
MS.gene09219.t1 MTR_1g035010 47.573 103 52 1 68 168 74 176 7.17e-24 103
MS.gene09219.t1 MTR_1g086250 56.250 80 35 0 96 175 147 226 1.08e-23 103
MS.gene09219.t1 MTR_7g110320 49.000 100 51 0 81 180 130 229 2.58e-23 102
MS.gene09219.t1 MTR_2g046550 57.895 76 32 0 96 171 139 214 2.85e-23 102
MS.gene09219.t1 MTR_7g110320 49.000 100 51 0 81 180 130 229 4.06e-23 101
MS.gene09219.t1 MTR_2g020620 34.524 168 91 5 103 263 97 252 3.66e-17 83.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09219.t1 AT1G27370 40.000 265 144 8 39 294 119 377 4.73e-45 159
MS.gene09219.t1 AT1G27370 40.000 265 144 8 39 294 119 377 4.73e-45 159
MS.gene09219.t1 AT1G27370 40.000 265 144 8 39 294 119 377 4.73e-45 159
MS.gene09219.t1 AT1G27370 40.000 265 144 8 39 294 119 377 4.73e-45 159
MS.gene09219.t1 AT1G27370 40.000 265 144 8 39 294 119 377 4.73e-45 159
MS.gene09219.t1 AT1G27370 40.000 265 144 8 39 294 119 377 4.73e-45 159
MS.gene09219.t1 AT1G27370 58.779 131 52 2 39 168 119 248 8.31e-44 155
MS.gene09219.t1 AT5G43270 61.194 134 50 2 36 168 109 241 1.26e-42 154
MS.gene09219.t1 AT5G43270 61.194 134 50 2 36 168 109 241 1.26e-42 154
MS.gene09219.t1 AT5G43270 61.194 134 50 2 36 168 109 241 1.26e-42 154
MS.gene09219.t1 AT1G27360 38.628 277 160 7 17 286 97 370 1.57e-41 150
MS.gene09219.t1 AT1G27360 38.628 277 160 7 17 286 97 370 1.57e-41 150
MS.gene09219.t1 AT1G27360 38.628 277 160 7 17 286 97 370 1.57e-41 150
MS.gene09219.t1 AT1G27360 38.628 277 160 7 17 286 97 370 1.57e-41 150
MS.gene09219.t1 AT2G42200 31.183 279 157 9 94 359 72 328 2.01e-31 122
MS.gene09219.t1 AT3G57920 31.210 314 164 13 47 350 27 298 1.05e-29 117
MS.gene09219.t1 AT1G69170 65.333 75 26 0 94 168 122 196 3.01e-29 117
MS.gene09219.t1 AT1G02065 46.923 130 59 1 45 174 147 266 2.39e-28 113
MS.gene09219.t1 AT2G33810 56.667 90 37 1 79 168 39 126 2.92e-27 105
MS.gene09219.t1 AT1G53160 61.644 73 28 0 96 168 54 126 1.99e-25 101
MS.gene09219.t1 AT1G53160 61.644 73 28 0 96 168 54 126 2.43e-25 101
MS.gene09219.t1 AT1G53160 61.644 73 28 0 96 168 54 126 2.43e-25 101
MS.gene09219.t1 AT3G15270 57.534 73 31 0 96 168 63 135 2.45e-24 98.6
MS.gene09219.t1 AT1G76580 53.933 89 41 0 94 182 112 200 2.56e-24 105
MS.gene09219.t1 AT1G76580 53.933 89 41 0 94 182 112 200 2.70e-24 105
MS.gene09219.t1 AT1G76580 53.933 89 41 0 94 182 112 200 3.84e-24 104
MS.gene09219.t1 AT5G50670 51.807 83 40 0 86 168 91 173 6.33e-24 101
MS.gene09219.t1 AT5G50670 51.807 83 40 0 86 168 91 173 6.33e-24 101
MS.gene09219.t1 AT5G50570 51.807 83 40 0 86 168 91 173 6.33e-24 101
MS.gene09219.t1 AT5G50570 51.807 83 40 0 86 168 91 173 6.33e-24 101
MS.gene09219.t1 AT3G60030 58.904 73 30 0 96 168 127 199 1.06e-23 103
MS.gene09219.t1 AT2G47070 60.274 73 29 0 96 168 106 178 1.50e-23 102
MS.gene09219.t1 AT1G20980 58.667 75 31 0 94 168 118 192 5.57e-23 101
MS.gene09219.t1 AT5G18830 42.727 110 53 2 59 168 111 210 2.96e-19 90.1
MS.gene09219.t1 AT5G18830 42.727 110 53 2 59 168 111 210 3.21e-19 89.7
MS.gene09219.t1 AT5G18830 42.727 110 53 2 59 168 111 210 3.51e-19 89.7
MS.gene09219.t1 AT1G02065 43.434 99 46 2 45 143 147 235 2.45e-17 80.9
MS.gene09219.t1 AT2G47070 58.333 48 20 0 121 168 2 49 1.23e-11 66.6

Find 64 sgRNAs with CRISPR-Local

Find 125 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGGATGTAAAATGGAAAT+TGG 0.200557 4.1:-15079165 None:intergenic
CATGATCTGTTTGAGTTTGA+TGG 0.225842 4.1:+15078065 MS.gene09219:CDS
CCAAACCTTTCTTGTGTATC+TGG 0.244468 4.1:+15077371 MS.gene09219:CDS
GAAGGACTTTGGATGTAAAA+TGG 0.246221 4.1:-15079172 None:intergenic
CTTGACCGAGAAATCAATTT+CGG 0.268342 4.1:+15077278 MS.gene09219:CDS
TTCAAAGAACCTTATGAGTA+TGG 0.284972 4.1:+15079557 MS.gene09219:CDS
TCAATCTAAGACTTGGTAAA+AGG 0.294020 4.1:+15077405 MS.gene09219:CDS
TCTGGCTCTGAGTTAATTCA+TGG 0.303523 4.1:+15077332 MS.gene09219:CDS
TATAACCACCATGGGAGATT+TGG 0.333181 4.1:-15077636 None:intergenic
ATAAAGCTGATTGGCTTGAT+TGG 0.333859 4.1:-15078160 None:intergenic
ATTAGAATGTTCTTCAGAAA+TGG 0.343450 4.1:-15079375 None:intergenic
GCAGTCTTGGAATTAGAAAA+TGG 0.356442 4.1:-15079020 None:intergenic
AGCTTTACCTGCTACATTGC+TGG 0.356873 4.1:-15077682 None:intergenic
TCTCCCATGGTGGTTATAGA+TGG 0.357918 4.1:+15077641 MS.gene09219:CDS
CATGGTGGTTATAGATGGTT+TGG 0.360015 4.1:+15077646 MS.gene09219:CDS
TTGACCGAGAAATCAATTTC+GGG 0.364920 4.1:+15077279 MS.gene09219:CDS
ACAACCCGAAATTGATTTCT+CGG 0.376943 4.1:-15077283 None:intergenic
CTGATTCATTGCATGAGTTA+TGG 0.392149 4.1:-15079420 None:intergenic
CCCGGTGGAAGTGGTTCTTC+TGG 0.397175 4.1:+15077314 MS.gene09219:CDS
GGCTAAATTTGTAGCAGTCT+TGG 0.413824 4.1:-15079033 None:intergenic
ACTCTTGGTTGGAAGGACTT+TGG 0.430559 4.1:-15079183 None:intergenic
CATCAAACTCAAACAGATCA+TGG 0.444519 4.1:-15078064 None:intergenic
CAGCTTGACGATGATATATA+CGG 0.462985 4.1:-15078136 None:intergenic
CCACCACGCTGCCAGGTTGA+AGG 0.471978 4.1:+15077548 MS.gene09219:CDS
CGCATATGAATCCCACTGAT+TGG 0.473627 4.1:-15079345 None:intergenic
CCAGATACACAAGAAAGGTT+TGG 0.481067 4.1:-15077371 None:intergenic
CTCTTGCTTCATCAGAGCCA+TGG 0.481784 4.1:+15079450 MS.gene09219:CDS
AACATTCATGTTAACCCAGC+TGG 0.488020 4.1:-15079586 None:intergenic
GATTGATACTCTTGGTTGGA+AGG 0.496148 4.1:-15079190 None:intergenic
TATGATACACCCGGTGGAAG+TGG 0.496488 4.1:+15077305 MS.gene09219:CDS
GTGAATAAGATAAAGCTGAT+TGG 0.502891 4.1:-15078169 None:intergenic
TGGTGAAAACCATACTCATA+AGG 0.504888 4.1:-15079566 None:intergenic
ACAATGTCCACCACGCTGCC+AGG 0.516200 4.1:+15077541 MS.gene09219:CDS
GCCAGAAGAACCACTTCCAC+CGG 0.517418 4.1:-15077315 None:intergenic
ACATTGTAAGTTCTGACCAT+TGG 0.517425 4.1:-15077525 None:intergenic
GAAAGCATATTGACAGTACT+TGG 0.532864 4.1:-15079134 None:intergenic
ATAACCTGGATTGATACTCT+TGG 0.533141 4.1:-15079198 None:intergenic
GATACACAAGAAAGGTTTGG+TGG 0.536044 4.1:-15077368 None:intergenic
CCTGGATTGATACTCTTGGT+TGG 0.537469 4.1:-15079194 None:intergenic
AACTTTCAAATCACAATGCA+AGG 0.545524 4.1:+15078111 MS.gene09219:CDS
AGTTATGGCTTGAAAAGCAC+TGG 0.554669 4.1:-15079405 None:intergenic
CCATTGCTCAATCTAAGACT+TGG 0.554906 4.1:+15077398 MS.gene09219:CDS
GTTCACCAGATACACAAGAA+AGG 0.558590 4.1:-15077376 None:intergenic
CCAACCAAGAGTATCAATCC+AGG 0.578039 4.1:+15079194 MS.gene09219:CDS
GAAAAGAAATGCAAGTCCAA+TGG 0.587403 4.1:+15077509 MS.gene09219:CDS
CCAAGTCTTAGATTGAGCAA+TGG 0.590002 4.1:-15077398 None:intergenic
CAAGAGCACAACCTTCAACC+TGG 0.591018 4.1:-15077559 None:intergenic
AATATGTCAATTCCAACATG+TGG 0.594822 4.1:+15079489 MS.gene09219:CDS
CTCTTCTGTCAACCAATCAG+TGG 0.597609 4.1:+15079333 MS.gene09219:CDS
TCTTCTGTCAACCAATCAGT+GGG 0.601400 4.1:+15079334 MS.gene09219:CDS
AGACTGCTACAAATTTAGCC+TGG 0.607461 4.1:+15079036 MS.gene09219:CDS
AAACCATCTATAACCACCAT+GGG 0.608088 4.1:-15077644 None:intergenic
CCAGAAGAACCACTTCCACC+GGG 0.608276 4.1:-15077314 None:intergenic
GCTTTATCTTATTCACGCAG+TGG 0.608852 4.1:+15078176 MS.gene09219:CDS
CCTTCAACCTGGCAGCGTGG+TGG 0.616962 4.1:-15077548 None:intergenic
AGAGTTTGAGATCCACATGT+TGG 0.619666 4.1:-15079501 None:intergenic
TTCGGGTTGTATGATACACC+CGG 0.621273 4.1:+15077296 MS.gene09219:CDS
CAAACCATCTATAACCACCA+TGG 0.633030 4.1:-15077645 None:intergenic
AAGTCATGCCAAATCTCCCA+TGG 0.638950 4.1:+15077628 MS.gene09219:CDS
CAACCTTCAACCTGGCAGCG+TGG 0.648763 4.1:-15077551 None:intergenic
CATGGCTCTGATGAAGCAAG+AGG 0.663007 4.1:-15079449 None:intergenic
TGATCATGATCACTACACCA+TGG 0.714032 4.1:-15079467 None:intergenic
TCATGCCAAATCTCCCATGG+TGG 0.722301 4.1:+15077631 MS.gene09219:CDS
GGGTTGTATGATACACCCGG+TGG 0.736684 4.1:+15077299 MS.gene09219:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATTTATTTCATAGATCTTAT+TGG + chr4.1:15078530-15078549 MS.gene09219:intron 15.0%
!!! TTCTATCTTCTAATATATTT+TGG + chr4.1:15078969-15078988 MS.gene09219:intron 15.0%
!! AATCTAGTTAGTAAATACTT+CGG - chr4.1:15078751-15078770 None:intergenic 20.0%
!! AGAATGTGTCAATTAATTAT+AGG - chr4.1:15077817-15077836 None:intergenic 20.0%
!! TAATAATAGTAGCTATCTTA+AGG - chr4.1:15077898-15077917 None:intergenic 20.0%
!!! AGGTTTATTATAGTTACTTT+TGG + chr4.1:15078876-15078895 MS.gene09219:intron 20.0%
!!! CAAAAATCTTTCTTTTTCAA+GGG + chr4.1:15077463-15077482 MS.gene09219:CDS 20.0%
!!! TAATGATAAGGTAATTTGTA+AGG - chr4.1:15077852-15077871 None:intergenic 20.0%
!!! TAGATAGTTGATTTAGTTAA+AGG + chr4.1:15078610-15078629 MS.gene09219:intron 20.0%
! AAAAAATCAGCACAAAAATG+TGG - chr4.1:15079237-15079256 None:intergenic 25.0%
! ATGAAATGTAGAAATCTAAG+AGG + chr4.1:15078286-15078305 MS.gene09219:intron 25.0%
! ATTAAGAAAGAAGAGAAATG+GGG - chr4.1:15077937-15077956 None:intergenic 25.0%
! ATTAGAATGTTCTTCAGAAA+TGG - chr4.1:15079378-15079397 None:intergenic 25.0%
! CATTAAGAAAGAAGAGAAAT+GGG - chr4.1:15077938-15077957 None:intergenic 25.0%
! TCATTAAGAAAGAAGAGAAA+TGG - chr4.1:15077939-15077958 None:intergenic 25.0%
! TTTGGATGTAAAATGGAAAT+TGG - chr4.1:15079168-15079187 None:intergenic 25.0%
!! AGGTAATTTGTAAGGTTATA+AGG - chr4.1:15077844-15077863 None:intergenic 25.0%
!! CCTTGAAAAAGAAAGATTTT+TGG - chr4.1:15077465-15077484 None:intergenic 25.0%
!!! AGTTGTATTTTTACTAAACC+AGG - chr4.1:15078799-15078818 None:intergenic 25.0%
!!! ATGATTAAACTTTTGAGTTG+TGG + chr4.1:15078585-15078604 MS.gene09219:intron 25.0%
!!! CCAAAAATCTTTCTTTTTCA+AGG + chr4.1:15077462-15077481 MS.gene09219:CDS 25.0%
!!! GTTACTTTTGGTTGTTTTAT+AGG + chr4.1:15078888-15078907 MS.gene09219:intron 25.0%
!!! TGATTTTTTTTTACTCTTGC+AGG + chr4.1:15079248-15079267 MS.gene09219:intron 25.0%
AAACTCTTGAGTAAATAAGC+AGG - chr4.1:15078409-15078428 None:intergenic 30.0%
AAATGAACAGAACTTACAAG+AGG - chr4.1:15078507-15078526 None:intergenic 30.0%
AACTTTCAAATCACAATGCA+AGG + chr4.1:15078111-15078130 MS.gene09219:CDS 30.0%
AATATGTCAATTCCAACATG+TGG + chr4.1:15079489-15079508 MS.gene09219:CDS 30.0%
ACATGGTTAAGATCCTAATT+GGG + chr4.1:15078639-15078658 MS.gene09219:intron 30.0%
ACCTATAAAACAACCAAAAG+TGG - chr4.1:15078860-15078879 None:intergenic 30.0%
CAGTAGCTATCTTAATGATA+AGG - chr4.1:15077864-15077883 None:intergenic 30.0%
CTTACAAGAGGTTAAAAGAT+AGG - chr4.1:15078495-15078514 None:intergenic 30.0%
GCAATGTTAATAGTCAACTA+AGG - chr4.1:15078332-15078351 None:intergenic 30.0%
GTGAATAAGATAAAGCTGAT+TGG - chr4.1:15078172-15078191 None:intergenic 30.0%
TTATCCTGATTGTTAGAGTT+TGG + chr4.1:15078697-15078716 MS.gene09219:intron 30.0%
TTCAAAGAACCTTATGAGTA+TGG + chr4.1:15079557-15079576 MS.gene09219:CDS 30.0%
! ATTTTCAATCGTCATACATC+TGG + chr4.1:15078553-15078572 MS.gene09219:intron 30.0%
! CACTTTTCTTAAAATGCTGA+AGG + chr4.1:15078039-15078058 MS.gene09219:intron 30.0%
! TCAATCTAAGACTTGGTAAA+AGG + chr4.1:15077405-15077424 MS.gene09219:CDS 30.0%
! TGGTAAAAGGATGTATTTTG+AGG + chr4.1:15077418-15077437 MS.gene09219:CDS 30.0%
! TTTGCGATGATAATCTTTTG+CGG - chr4.1:15077594-15077613 None:intergenic 30.0%
!! AATCTAATTCAACTTGCTGT+TGG + chr4.1:15077989-15078008 MS.gene09219:intron 30.0%
!! TCCACTTTTGGTTGTTTTAT+AGG + chr4.1:15078856-15078875 MS.gene09219:intron 30.0%
!!! AGATTTTTGGAATCACTTTC+TGG - chr4.1:15077452-15077471 None:intergenic 30.0%
AAACCATCTATAACCACCAT+GGG - chr4.1:15077647-15077666 None:intergenic 35.0%
AACCAAAAGTGGATGTTCTT+TGG - chr4.1:15078849-15078868 None:intergenic 35.0%
ATAACCTGGATTGATACTCT+TGG - chr4.1:15079201-15079220 None:intergenic 35.0%
ATGTCCAAACTCTAACAATC+AGG - chr4.1:15078704-15078723 None:intergenic 35.0%
CAGCTTGACGATGATATATA+CGG - chr4.1:15078139-15078158 None:intergenic 35.0%
CATCAAACTCAAACAGATCA+TGG - chr4.1:15078067-15078086 None:intergenic 35.0%
CATGGTTAAGATCCTAATTG+GGG + chr4.1:15078640-15078659 MS.gene09219:intron 35.0%
CTAAACCAGGTATTAACGAA+TGG - chr4.1:15078786-15078805 None:intergenic 35.0%
CTGATTCATTGCATGAGTTA+TGG - chr4.1:15079423-15079442 None:intergenic 35.0%
CTTGACCGAGAAATCAATTT+CGG + chr4.1:15077278-15077297 MS.gene09219:CDS 35.0%
GAAAAGAAATGCAAGTCCAA+TGG + chr4.1:15077509-15077528 MS.gene09219:CDS 35.0%
GAAGGACTTTGGATGTAAAA+TGG - chr4.1:15079175-15079194 None:intergenic 35.0%
GACATGGTTAAGATCCTAAT+TGG + chr4.1:15078638-15078657 MS.gene09219:intron 35.0%
GCAATAAACGTGAAATAACC+TGG - chr4.1:15079215-15079234 None:intergenic 35.0%
GCAGTCTTGGAATTAGAAAA+TGG - chr4.1:15079023-15079042 None:intergenic 35.0%
GTTAGTAAATACTTCGGCTT+AGG - chr4.1:15078745-15078764 None:intergenic 35.0%
TGGTGAAAACCATACTCATA+AGG - chr4.1:15079569-15079588 None:intergenic 35.0%
TTGACCGAGAAATCAATTTC+GGG + chr4.1:15077279-15077298 MS.gene09219:CDS 35.0%
! AAGGGAAAATCTTTTGCTTG+GGG - chr4.1:15079086-15079105 None:intergenic 35.0%
! ACAACCCGAAATTGATTTCT+CGG - chr4.1:15077286-15077305 None:intergenic 35.0%
! ACATTGTAAGTTCTGACCAT+TGG - chr4.1:15077528-15077547 None:intergenic 35.0%
! CAAGGGAAAATCTTTTGCTT+GGG - chr4.1:15079087-15079106 None:intergenic 35.0%
! CATGATCTGTTTGAGTTTGA+TGG + chr4.1:15078065-15078084 MS.gene09219:CDS 35.0%
! GAAAGCATATTGACAGTACT+TGG - chr4.1:15079137-15079156 None:intergenic 35.0%
! TCAAGGGAAAATCTTTTGCT+TGG - chr4.1:15079088-15079107 None:intergenic 35.0%
! TTAGTTAAAGGCACAAGACA+TGG + chr4.1:15078622-15078641 MS.gene09219:intron 35.0%
!! ATAAAGCTGATTGGCTTGAT+TGG - chr4.1:15078163-15078182 None:intergenic 35.0%
AGACTGCTACAAATTTAGCC+TGG + chr4.1:15079036-15079055 MS.gene09219:CDS 40.0%
CAAACCATCTATAACCACCA+TGG - chr4.1:15077648-15077667 None:intergenic 40.0%
CAACTAAGGCATGCATAACA+TGG - chr4.1:15078318-15078337 None:intergenic 40.0%
CATGGTGGTTATAGATGGTT+TGG + chr4.1:15077646-15077665 MS.gene09219:CDS 40.0%
CCAAACCTTTCTTGTGTATC+TGG + chr4.1:15077371-15077390 MS.gene09219:CDS 40.0%
CCAAGTCTTAGATTGAGCAA+TGG - chr4.1:15077401-15077420 None:intergenic 40.0%
CCAGATACACAAGAAAGGTT+TGG - chr4.1:15077374-15077393 None:intergenic 40.0%
GATACACAAGAAAGGTTTGG+TGG - chr4.1:15077371-15077390 None:intergenic 40.0%
GATTGATACTCTTGGTTGGA+AGG - chr4.1:15079193-15079212 None:intergenic 40.0%
GCTTTATCTTATTCACGCAG+TGG + chr4.1:15078176-15078195 MS.gene09219:CDS 40.0%
GGCTAAATTTGTAGCAGTCT+TGG - chr4.1:15079036-15079055 None:intergenic 40.0%
GTATGCCATTCGTTAATACC+TGG + chr4.1:15078778-15078797 MS.gene09219:intron 40.0%
GTTCACCAGATACACAAGAA+AGG - chr4.1:15077379-15077398 None:intergenic 40.0%
TATAACCACCATGGGAGATT+TGG - chr4.1:15077639-15077658 None:intergenic 40.0%
TCAATCGTCATACATCTGGA+TGG + chr4.1:15078557-15078576 MS.gene09219:intron 40.0%
TCTTCTGTCAACCAATCAGT+GGG + chr4.1:15079334-15079353 MS.gene09219:CDS 40.0%
TGATCATGATCACTACACCA+TGG - chr4.1:15079470-15079489 None:intergenic 40.0%
! AAGAGTATGCTACCCCAATT+AGG - chr4.1:15078655-15078674 None:intergenic 40.0%
! AATGTCTCGTGTAACTTGCA+AGG - chr4.1:15078433-15078452 None:intergenic 40.0%
! AGAGTTTGAGATCCACATGT+TGG - chr4.1:15079504-15079523 None:intergenic 40.0%
! AGGGAAAATCTTTTGCTTGG+GGG - chr4.1:15079085-15079104 None:intergenic 40.0%
! AGTTATGGCTTGAAAAGCAC+TGG - chr4.1:15079408-15079427 None:intergenic 40.0%
! CCATTGCTCAATCTAAGACT+TGG + chr4.1:15077398-15077417 MS.gene09219:CDS 40.0%
! GACCAAAGAACATCCACTTT+TGG + chr4.1:15078844-15078863 MS.gene09219:intron 40.0%
! GTGTCAATGTTGTCTTTTGC+AGG - chr4.1:15079113-15079132 None:intergenic 40.0%
! TTGTCTTTTGCAGGCTTCAA+GGG - chr4.1:15079104-15079123 None:intergenic 40.0%
!! TCTGGCTCTGAGTTAATTCA+TGG + chr4.1:15077332-15077351 MS.gene09219:CDS 40.0%
AAGTCATGCCAAATCTCCCA+TGG + chr4.1:15077628-15077647 MS.gene09219:CDS 45.0%
ACTCTTGGTTGGAAGGACTT+TGG - chr4.1:15079186-15079205 None:intergenic 45.0%
AGCTTTACCTGCTACATTGC+TGG - chr4.1:15077685-15077704 None:intergenic 45.0%
CCTGGATTGATACTCTTGGT+TGG - chr4.1:15079197-15079216 None:intergenic 45.0%
CGCATATGAATCCCACTGAT+TGG - chr4.1:15079348-15079367 None:intergenic 45.0%
CTCTTCTGTCAACCAATCAG+TGG + chr4.1:15079333-15079352 MS.gene09219:CDS 45.0%
TCTCCCATGGTGGTTATAGA+TGG + chr4.1:15077641-15077660 MS.gene09219:CDS 45.0%
! GAGTATGGTTTTCACCAGCT+GGG + chr4.1:15079572-15079591 MS.gene09219:CDS 45.0%
! GATTTTGCCAGCAATGTAGC+AGG + chr4.1:15077675-15077694 MS.gene09219:CDS 45.0%
! GTTGTCTTTTGCAGGCTTCA+AGG - chr4.1:15079105-15079124 None:intergenic 45.0%
! TGAGTATGGTTTTCACCAGC+TGG + chr4.1:15079571-15079590 MS.gene09219:CDS 45.0%
!! CCAACCAAGAGTATCAATCC+AGG + chr4.1:15079194-15079213 MS.gene09219:CDS 45.0%
!! TTCGGGTTGTATGATACACC+CGG + chr4.1:15077296-15077315 MS.gene09219:CDS 45.0%
AGCAGGACAGCAAAGAAGAG+TGG - chr4.1:15078392-15078411 None:intergenic 50.0%
CTCTTGCTTCATCAGAGCCA+TGG + chr4.1:15079450-15079469 MS.gene09219:CDS 50.0%
TATGATACACCCGGTGGAAG+TGG + chr4.1:15077305-15077324 MS.gene09219:CDS 50.0%
TCATGCCAAATCTCCCATGG+TGG + chr4.1:15077631-15077650 MS.gene09219:CDS 50.0%
! CAAGAGCACAACCTTCAACC+TGG - chr4.1:15077562-15077581 None:intergenic 50.0%
! GCTGTTGTGCATGTTTTGCC+AGG - chr4.1:15079057-15079076 None:intergenic 50.0%
!! CATGGCTCTGATGAAGCAAG+AGG - chr4.1:15079452-15079471 None:intergenic 50.0%
CCAGAAGAACCACTTCCACC+GGG - chr4.1:15077317-15077336 None:intergenic 55.0%
GCCAGAAGAACCACTTCCAC+CGG - chr4.1:15077318-15077337 None:intergenic 55.0%
GGGTTGTATGATACACCCGG+TGG + chr4.1:15077299-15077318 MS.gene09219:CDS 55.0%
ACAATGTCCACCACGCTGCC+AGG + chr4.1:15077541-15077560 MS.gene09219:CDS 60.0%
CAACCTTCAACCTGGCAGCG+TGG - chr4.1:15077554-15077573 None:intergenic 60.0%
CCCGGTGGAAGTGGTTCTTC+TGG + chr4.1:15077314-15077333 MS.gene09219:CDS 60.0%
CCTTCAACCTGGCAGCGTGG+TGG - chr4.1:15077551-15077570 None:intergenic 65.0%
! CCACCACGCTGCCAGGTTGA+AGG + chr4.1:15077548-15077567 MS.gene09219:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 15077272 15079598 15077272 ID=MS.gene09219
chr4.1 mRNA 15077272 15079598 15077272 ID=MS.gene09219.t1;Parent=MS.gene09219
chr4.1 exon 15077272 15077696 15077272 ID=MS.gene09219.t1.exon1;Parent=MS.gene09219.t1
chr4.1 CDS 15077272 15077696 15077272 ID=cds.MS.gene09219.t1;Parent=MS.gene09219.t1
chr4.1 exon 15078061 15078197 15078061 ID=MS.gene09219.t1.exon2;Parent=MS.gene09219.t1
chr4.1 CDS 15078061 15078197 15078061 ID=cds.MS.gene09219.t1;Parent=MS.gene09219.t1
chr4.1 exon 15079000 15079215 15079000 ID=MS.gene09219.t1.exon3;Parent=MS.gene09219.t1
chr4.1 CDS 15079000 15079215 15079000 ID=cds.MS.gene09219.t1;Parent=MS.gene09219.t1
chr4.1 exon 15079270 15079598 15079270 ID=MS.gene09219.t1.exon4;Parent=MS.gene09219.t1
chr4.1 CDS 15079270 15079598 15079270 ID=cds.MS.gene09219.t1;Parent=MS.gene09219.t1
Gene Sequence

>MS.gene09219

ATGGAACTTGACCGAGAAATCAATTTCGGGTTGTATGATACACCCGGTGGAAGTGGTTCTTCTGGCTCTGAGTTAATTCATGGTTCAGCATTTAATCCACCAAACCTTTCTTGTGTATCTGGTGAACCATTGCTCAATCTAAGACTTGGTAAAAGGATGTATTTTGAGGAAGTTTGTCCAGAAAGTGATTCCAAAAATCTTTCTTTTTCAAGGGATCTCATGTCATCTTCAATTCTTGAAAAGAAATGCAAGTCCAATGGTCAGAACTTACAATGTCCACCACGCTGCCAGGTTGAAGGTTGTGCTCTTGATCTTTCATCCGCAAAAGATTATCATCGCAAACGAAGAGTTTGTGAAAGTCATGCCAAATCTCCCATGGTGGTTATAGATGGTTTGGAGCGTCGATTTTGCCAGCAATGTAGCAGGTAAAGCTTCTTAACAACTGTATAATTGTTTAGATCTAATTGACATAGAAAATAATAGTATTTTTTTCGAATGATGAATTCTTGTTAGCACATCATTAAAAGCTAATATTACTATTACCTATAATTAATTGACACATTCTGTTACCTTATAACCTTACAAATTACCTTATCATTAAGATAGCTACTGTTACATTGTAACCTTAAGATAGCTACTATTATTAATGAGAAAATTATTCTCCCCATTTCTCTTCTTTCTTAATGAAGTTTTTTTCACATTCATCAATTACTACTCAATCTAATTCAACTTGCTGTTGGTGTTGAATATATTGCTCTAGAATTGATCACTTTTCTTAAAATGCTGAAGGTTCCATGATCTGTTTGAGTTTGATGGAAAGAAGAAAAGCTGCAGACGACAACTTTCAAATCACAATGCAAGGCGCCGTATATATCATCGTCAAGCTGTCCAATCAAGCCAATCAGCTTTATCTTATTCACGCAGTGGTAATACTTATTTTTCTCTGATTTTGCAGTTTATCTCAGTTCATGCTTGATTGCTTGTTATCTGTTTTTGATTGTTAAAGCTGAAAAAATGAAATGTAGAAATCTAAGAGGCTAAGACCATGTTATGCATGCCTTAGTTGACTATTAACATTGCTGAATGAAAACTGTTTGAAAAATTGAAATGCTTAACACCACTCTTCTTTGCTGTCCTGCTTATTTACTCAAGAGTTTGCCTTGCAAGTTACACGAGACATTAAATGTAATGCAACAGAACTTCTTAATTTTCTACAGTTTCCTATCTTTTAACCTCTTGTAAGTTCTGTTCATTTTTCATTTATTTCATAGATCTTATTGGATTTTCAATCGTCATACATCTGGATGGTAAACATGATTAAACTTTTGAGTTGTGGACTAGATAGTTGATTTAGTTAAAGGCACAAGACATGGTTAAGATCCTAATTGGGGTAGCATACTCTTGATTACAGAATTACACAAATCATTATCCTGATTGTTAGAGTTTGGACATGAGCGCGAGTATAACATTCCTAAGCCGAAGTATTTACTAACTAGATTTGTATGAGTATGCCATTCGTTAATACCTGGTTTAGTAAAAATACAACTTCAAATTGCTGTTAGTCAAAACTTAGACCAAAGAACATCCACTTTTGGTTGTTTTATAGGTTTATTATAGTTACTTTTGGTTGTTTTATAGGTTTATTATAGTTAATTGCAAGTCATTCATTTCTCAATGATTCAAATTTTACTGTTGTCTTCTATCTTCTAATATATTTTGGTTCTGCAGATGAGAAGCAACAGATGAGTCCATTTTCTAATTCCAAGACTGCTACAAATTTAGCCTGGCAAAACATGCACAACAGCAAGCTCCCCCAAGCAAAAGATTTTCCCTTGAAGCCTGCAAAAGACAACATTGACACACCAAGTACTGTCAATATGCTTTCTCATGATTCCAATTTCCATTTTACATCCAAAGTCCTTCCAACCAAGAGTATCAATCCAGGTTATTTCACGTTTATTGCCACATTTTTGTGCTGATTTTTTTTTACTCTTGCAGGTGTAGAAGATTTCATGAACTTTTCTGATACAAATGCTACACAAGACTTTACTTGTGCTCTCTCTCTTCTGTCAACCAATCAGTGGGATTCATATGCGACTAAATCCATTTCTGAAGAACATTCTAATCGCTCGACCAGTGCTTTTCAAGCCATAACTCATGCAATGAATCAGCGCATTCCTCTTGCTTCATCAGAGCCATGGTGTAGTGATCATGATCAATATGTCAATTCCAACATGTGGATCTCAAACTCTGCAGATAGCAATCACTTTCAGAAATTTCAACTGTTCAAAGAACCTTATGAGTATGGTTTTCACCAGCTGGGTTAA
Protein sequence

>MS.gene09219.t1

MELDREINFGLYDTPGGSGSSGSELIHGSAFNPPNLSCVSGEPLLNLRLGKRMYFEEVCPESDSKNLSFSRDLMSSSILEKKCKSNGQNLQCPPRCQVEGCALDLSSAKDYHRKRRVCESHAKSPMVVIDGLERRFCQQCSRFHDLFEFDGKKKSCRRQLSNHNARRRIYHRQAVQSSQSALSYSRSDEKQQMSPFSNSKTATNLAWQNMHNSKLPQAKDFPLKPAKDNIDTPSTVNMLSHDSNFHFTSKVLPTKSINPGVEDFMNFSDTNATQDFTCALSLLSTNQWDSYATKSISEEHSNRSTSAFQAITHAMNQRIPLASSEPWCSDHDQYVNSNMWISNSADSNHFQKFQLFKEPYEYGFHQLG