AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene09220

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09220.t1 MTR_8g080690 84.043 376 26 2 1 342 1 376 0.0 576
MS.gene09220.t1 MTR_8g080680 62.269 379 85 7 1 342 1 358 1.00e-149 427
MS.gene09220.t1 MTR_8g080670 56.436 404 98 8 1 342 1 388 2.74e-122 358
MS.gene09220.t1 MTR_3g085180 45.524 391 156 9 1 341 1 384 2.23e-91 280
MS.gene09220.t1 MTR_1g053715 37.945 253 119 7 105 322 25 274 5.63e-37 136
MS.gene09220.t1 MTR_7g092930 40.201 199 85 6 144 314 61 253 6.35e-33 125
MS.gene09220.t1 MTR_3g099080 52.252 111 52 1 151 261 98 207 2.14e-32 124
MS.gene09220.t1 MTR_8g463140 66.250 80 27 0 149 228 63 142 4.83e-32 118
MS.gene09220.t1 MTR_8g096780 39.796 196 100 6 137 328 85 266 7.10e-31 120
MS.gene09220.t1 MTR_7g444860 46.405 153 70 4 99 242 98 247 8.54e-30 116
MS.gene09220.t1 MTR_2g078770 64.474 76 27 0 149 224 56 131 1.69e-29 111
MS.gene09220.t1 MTR_4g109770 40.437 183 83 6 151 326 123 286 1.86e-29 118
MS.gene09220.t1 MTR_5g046670 56.977 86 37 0 149 234 242 327 2.93e-28 115
MS.gene09220.t1 MTR_7g028740 45.614 114 61 1 151 264 77 189 5.28e-28 112
MS.gene09220.t1 MTR_4g088555 63.158 76 28 0 149 224 61 136 8.27e-28 106
MS.gene09220.t1 MTR_2g461920 55.556 90 40 0 137 226 157 246 1.14e-27 113
MS.gene09220.t1 MTR_8g005960 64.103 78 28 0 149 226 185 262 1.22e-27 110
MS.gene09220.t1 MTR_1g035010 57.831 83 35 0 148 230 101 183 5.04e-27 112
MS.gene09220.t1 MTR_2g014200 56.250 80 35 0 149 228 62 141 8.67e-26 100
MS.gene09220.t1 MTR_1g086250 53.684 95 44 0 136 230 132 226 2.06e-25 107
MS.gene09220.t1 MTR_2g046550 58.974 78 32 0 151 228 139 216 1.34e-24 105
MS.gene09220.t1 MTR_7g110320 55.294 85 38 0 151 235 145 229 9.17e-24 102
MS.gene09220.t1 MTR_7g110320 55.294 85 38 0 151 235 145 229 1.01e-23 102
MS.gene09220.t1 MTR_2g020620 38.655 119 59 2 158 269 97 208 1.19e-17 84.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09220.t1 AT1G27360 44.223 251 114 8 1 226 1 250 2.62e-48 167
MS.gene09220.t1 AT1G27360 44.223 251 114 8 1 226 1 250 2.62e-48 167
MS.gene09220.t1 AT1G27360 44.223 251 114 8 1 226 1 250 2.62e-48 167
MS.gene09220.t1 AT1G27360 44.223 251 114 8 1 226 1 250 2.62e-48 167
MS.gene09220.t1 AT5G43270 38.021 384 176 16 1 333 1 373 1.13e-47 166
MS.gene09220.t1 AT5G43270 38.021 384 176 16 1 333 1 373 1.13e-47 166
MS.gene09220.t1 AT5G43270 38.021 384 176 16 1 333 1 373 1.13e-47 166
MS.gene09220.t1 AT1G27370 41.729 266 128 6 1 240 1 265 1.83e-46 161
MS.gene09220.t1 AT1G27370 41.729 266 128 6 1 240 1 265 1.04e-45 160
MS.gene09220.t1 AT1G27370 41.729 266 128 6 1 240 1 265 1.04e-45 160
MS.gene09220.t1 AT1G27370 41.729 266 128 6 1 240 1 265 1.04e-45 160
MS.gene09220.t1 AT1G27370 41.729 266 128 6 1 240 1 265 1.04e-45 160
MS.gene09220.t1 AT1G27370 41.729 266 128 6 1 240 1 265 1.04e-45 160
MS.gene09220.t1 AT1G27370 41.729 266 128 6 1 240 1 265 1.04e-45 160
MS.gene09220.t1 AT2G42200 54.955 111 45 2 148 258 71 176 6.94e-33 125
MS.gene09220.t1 AT1G69170 64.103 78 28 0 147 224 120 197 1.69e-29 117
MS.gene09220.t1 AT5G50670 57.143 84 36 0 147 230 97 180 2.10e-28 113
MS.gene09220.t1 AT5G50670 57.143 84 36 0 147 230 97 180 2.10e-28 113
MS.gene09220.t1 AT5G50570 57.143 84 36 0 147 230 97 180 2.10e-28 113
MS.gene09220.t1 AT5G50570 57.143 84 36 0 147 230 97 180 2.10e-28 113
MS.gene09220.t1 AT1G76580 55.914 93 41 0 148 240 111 203 2.45e-28 116
MS.gene09220.t1 AT1G76580 55.914 93 41 0 148 240 111 203 2.45e-28 116
MS.gene09220.t1 AT1G76580 55.914 93 41 0 148 240 111 203 3.55e-28 115
MS.gene09220.t1 AT1G02065 49.593 123 52 2 105 226 151 264 5.74e-28 111
MS.gene09220.t1 AT3G57920 40.351 171 85 5 150 304 58 227 1.89e-27 110
MS.gene09220.t1 AT2G33810 60.811 74 29 0 151 224 54 127 9.57e-27 103
MS.gene09220.t1 AT3G60030 55.814 86 38 0 151 236 127 212 1.23e-26 111
MS.gene09220.t1 AT1G20980 53.465 101 46 1 137 236 106 206 1.89e-26 110
MS.gene09220.t1 AT2G47070 61.538 78 30 0 151 228 106 183 1.44e-25 108
MS.gene09220.t1 AT1G53160 56.962 79 34 0 151 229 54 132 3.57e-25 100
MS.gene09220.t1 AT1G53160 56.962 79 34 0 151 229 54 132 4.70e-25 100
MS.gene09220.t1 AT1G53160 56.962 79 34 0 151 229 54 132 4.70e-25 100
MS.gene09220.t1 AT3G15270 56.757 74 32 0 151 224 63 136 3.56e-24 97.8
MS.gene09220.t1 AT5G18830 52.000 75 36 0 150 224 137 211 6.56e-20 91.3
MS.gene09220.t1 AT5G18830 52.000 75 36 0 150 224 137 211 6.80e-20 91.3
MS.gene09220.t1 AT5G18830 52.000 75 36 0 150 224 137 211 7.36e-20 91.3
MS.gene09220.t1 AT1G02065 44.681 94 43 1 105 198 151 235 9.04e-17 79.0
MS.gene09220.t1 AT2G47070 60.377 53 21 0 176 228 2 54 1.33e-13 72.4

Find 57 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGCTTGGTGGTTGTAATTT+TGG 0.089177 4.1:-15072452 None:intergenic
CAAATAGTTCATCGTCAAAT+AGG 0.221746 4.1:+15072573 MS.gene09220:CDS
TGACTTGATTGGAGTGCTTC+AGG 0.279073 4.1:-15073235 None:intergenic
CTCCCTCTTCCCAGTGGGAC+TGG 0.294118 4.1:+15072390 MS.gene09220:CDS
CTTGAGTCATCTTTAAGTAT+TGG 0.332725 4.1:+15072755 MS.gene09220:CDS
TATAACCACCATAGGAGATT+TGG 0.339545 4.1:-15072900 None:intergenic
AGCTTTACCTGCCACATTGC+TGG 0.352012 4.1:-15072946 None:intergenic
ACAAAACTGATTGACTTGAT+TGG 0.364968 4.1:-15073246 None:intergenic
TCCCTCTTCCCAGTGGGACT+GGG 0.389604 4.1:+15072391 MS.gene09220:CDS
CCAGAGCTTTCTTCTGTCTC+TGG 0.403530 4.1:+15072641 MS.gene09220:CDS
TCTCCTATGGTGGTTATAGA+TGG 0.407218 4.1:+15072905 MS.gene09220:CDS
ACAACCCGGCATTGATTTCT+CGG 0.409997 4.1:-15072490 None:intergenic
CTTGCAATTTCCGCGCTGCC+AGG 0.416641 4.1:+15072805 MS.gene09220:CDS
AAATAGTTCATCGTCAAATA+GGG 0.430475 4.1:+15072574 MS.gene09220:CDS
CGCTGCCAGGTTGAAGGTTG+TGG 0.443635 4.1:+15072818 MS.gene09220:CDS
TTTCCGCGCTGCCAGGTTGA+AGG 0.450558 4.1:+15072812 MS.gene09220:CDS
ATTGGTGCTCCCAGTCCCAC+TGG 0.471654 4.1:-15072400 None:intergenic
CTCCCAGTCCCACTGGGAAG+AGG 0.474118 4.1:-15072393 None:intergenic
TGTTTGATGCTCCAGCTTGG+TGG 0.481479 4.1:-15072464 None:intergenic
TTTATCATTTGTATCAGAAA+CGG 0.482839 4.1:-15074451 None:intergenic
CAAAATTACAACCACCAAGC+TGG 0.484604 4.1:+15072453 MS.gene09220:CDS
TATGGTGGTTATAGATGGTA+TGG 0.485704 4.1:+15072910 MS.gene09220:CDS
CTCTTCTGTCAACTAATCCA+TGG 0.493619 4.1:+15074496 MS.gene09220:CDS
GCAGTCTCTGAATTAGCAAA+TGG 0.494415 4.1:-15074206 None:intergenic
TCTTGTTTGATGCTCCAGCT+TGG 0.510678 4.1:-15072467 None:intergenic
AAGTCATTCCAAATCTCCTA+TGG 0.520495 4.1:+15072892 MS.gene09220:CDS
CTGACTCATTGCATGAGTTA+CGG 0.521872 4.1:-15074583 None:intergenic
GAACCATTGCTCACTCTACT+CGG 0.540591 4.1:+15072665 MS.gene09220:CDS
CATGATTCTGATTAAACAAG+AGG 0.549250 4.1:-15074612 None:intergenic
CAAGACCGAGAAATCAATGC+CGG 0.550620 4.1:+15072485 MS.gene09220:CDS
TCCCAGTCCCACTGGGAAGA+GGG 0.552029 4.1:-15072392 None:intergenic
AACTTTCACATCACAATGCA+AGG 0.562057 4.1:+15073197 MS.gene09220:CDS
TCTTCTGTCAACTAATCCAT+GGG 0.562513 4.1:+15074497 MS.gene09220:CDS
CATCGAACTCAAACAGATCG+TGG 0.568188 4.1:-15073150 None:intergenic
GATAATCTTTCGCTGATGAA+AGG 0.568732 4.1:-15072847 None:intergenic
CCAGAGACAGAAGAAAGCTC+TGG 0.576248 4.1:-15072641 None:intergenic
ATTAGAATGTTCTAGAGACA+TGG 0.583823 4.1:-15074538 None:intergenic
AGTTACGGCTTGAACAGCAC+TGG 0.590790 4.1:-15074568 None:intergenic
CAAGGCCACAACCTTCAACC+TGG 0.591428 4.1:-15072823 None:intergenic
ACGGCTTGAACAGCACTGGT+TGG 0.597812 4.1:-15074564 None:intergenic
TTAGTCGCATATGAATCCCA+TGG 0.598308 4.1:-15074513 None:intergenic
GCCGGGTTGTTCGATACACC+TGG 0.605439 4.1:+15072503 MS.gene09220:CDS
AAGACCGAGAAATCAATGCC+GGG 0.606643 4.1:+15072486 MS.gene09220:CDS
AGACTGCTACAAATTTAGCC+TGG 0.607461 4.1:+15074222 MS.gene09220:CDS
ATACCATCTATAACCACCAT+AGG 0.609763 4.1:-15072908 None:intergenic
TTGGTGCTCCCAGTCCCACT+GGG 0.623897 4.1:-15072399 None:intergenic
TTTCCGAGTAGAGTGAGCAA+TGG 0.627280 4.1:-15072668 None:intergenic
CTTTCGCTGATGAAAGGTCA+AGG 0.632145 4.1:-15072841 None:intergenic
TTAGCCTGGCAAAACATGAA+CGG 0.633733 4.1:+15074236 MS.gene09220:CDS
AATATCTCCCTCTTCCCAGT+GGG 0.634960 4.1:+15072385 MS.gene09220:CDS
CAATATCTCCCTCTTCCCAG+TGG 0.638747 4.1:+15072384 MS.gene09220:CDS
TCATTCCAAATCTCCTATGG+TGG 0.656270 4.1:+15072895 MS.gene09220:CDS
CAACCTTCAACCTGGCAGCG+CGG 0.659706 4.1:-15072815 None:intergenic
CGGAAAACGAGTGTACATTG+AGG 0.675568 4.1:+15072685 MS.gene09220:CDS
GGGTTGTTCGATACACCTGG+TGG 0.681597 4.1:+15072506 MS.gene09220:CDS
AAAGACAACACTAACACACA+AGG 0.691256 4.1:+15074278 MS.gene09220:CDS
ACCAGGTGTATCGAACAACC+CGG 0.701562 4.1:-15072504 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAATTTTTTTATTTTTTTTT+TGG + chr4.1:15073013-15073032 MS.gene09220:intron 0.0%
!! AAATAATTATCATGATTGTT+TGG + chr4.1:15073891-15073910 MS.gene09220:intron 15.0%
!!! ATTCACTTTTAGTTTTTTAT+AGG + chr4.1:15074052-15074071 MS.gene09220:intron 15.0%
!!! GTTTATTGTAGTTTTTTTAT+AGG + chr4.1:15074074-15074093 MS.gene09220:intron 15.0%
!!! TTCTAACTTCTAATATATTT+TGG + chr4.1:15074155-15074174 MS.gene09220:intron 15.0%
!! ATAATTACTTATTGTCAGAA+AGG + chr4.1:15073755-15073774 MS.gene09220:intron 20.0%
!! TTTATCATTTGTATCAGAAA+CGG - chr4.1:15074454-15074473 None:intergenic 20.0%
!!! GTTGTTTTTTTTACAAAATC+AGG - chr4.1:15073993-15074012 None:intergenic 20.0%
!!! TTTAGTTTCATAGATCTTAT+TGG + chr4.1:15073599-15073618 MS.gene09220:intron 20.0%
! AAAAATCAGCACAAAAAATG+TGG - chr4.1:15074400-15074419 None:intergenic 25.0%
! AAATAGTTCATCGTCAAATA+GGG + chr4.1:15072574-15072593 MS.gene09220:CDS 25.0%
! AACTAAAAGTGAATGTTCTT+TGG - chr4.1:15074046-15074065 None:intergenic 25.0%
! ATATTGCTATATTAGAGTTG+TGG + chr4.1:15073778-15073797 MS.gene09220:intron 25.0%
! TAACAAGAGCTTAAAAAGAT+AGG - chr4.1:15073562-15073581 None:intergenic 25.0%
! TATTGCTATATTAGAGTTGT+GGG + chr4.1:15073779-15073798 MS.gene09220:intron 25.0%
! TGTATCATATGCCTTAATAA+GGG - chr4.1:15073872-15073891 None:intergenic 25.0%
! TGTTTCAATGTAATTGACAT+AGG + chr4.1:15072987-15073006 MS.gene09220:intron 25.0%
! TTGTATCATATGCCTTAATA+AGG - chr4.1:15073873-15073892 None:intergenic 25.0%
!!! AAAACATGTATTTTTGCTTG+TGG + chr4.1:15072605-15072624 MS.gene09220:CDS 25.0%
!!! AGGTTTTTGGAATTACTTTT+CGG - chr4.1:15072719-15072738 None:intergenic 25.0%
!!! CAAAAACCTATCTTTTTCAA+GGG + chr4.1:15072730-15072749 MS.gene09220:CDS 25.0%
!!! CAAAATCAGGTTTTAAAGAA+TGG - chr4.1:15073980-15073999 None:intergenic 25.0%
!!! CTGATTTTTTATAACTTTGC+AGG + chr4.1:15074411-15074430 MS.gene09220:intron 25.0%
AAACGTGAAATAACCTCAAT+TGG - chr4.1:15074373-15074392 None:intergenic 30.0%
AAAGCTGAAAAGATGAAATG+TGG + chr4.1:15073349-15073368 MS.gene09220:intron 30.0%
CAAATAGTTCATCGTCAAAT+AGG + chr4.1:15072573-15072592 MS.gene09220:CDS 30.0%
CAAGAACTTTGGATGTAAAA+TGG - chr4.1:15074337-15074356 None:intergenic 30.0%
CTTGAGTCATCTTTAAGTAT+TGG + chr4.1:15072755-15072774 MS.gene09220:CDS 30.0%
GCAATGTTTATTGTCAACTA+AGG - chr4.1:15073399-15073418 None:intergenic 30.0%
GTATCATATGCCTTAATAAG+GGG - chr4.1:15073871-15073890 None:intergenic 30.0%
TTTGGATGTAAAATGGACAA+CGG - chr4.1:15074330-15074349 None:intergenic 30.0%
! CCTAAGTCTTTAGAGAATTA+AGG + chr4.1:15073838-15073857 MS.gene09220:intron 30.0%
! CCTTAATTCTCTAAAGACTT+AGG - chr4.1:15073841-15073860 None:intergenic 30.0%
! CCTTGAAAAAGATAGGTTTT+TGG - chr4.1:15072732-15072751 None:intergenic 30.0%
! TAACCTCAATTGGATACTTT+TGG - chr4.1:15074363-15074382 None:intergenic 30.0%
! TGTTAGTGTTGTCTTTTGTT+TGG - chr4.1:15074274-15074293 None:intergenic 30.0%
!! ACAAAACTGATTGACTTGAT+TGG - chr4.1:15073249-15073268 None:intergenic 30.0%
!! ATTAGAATGTTCTAGAGACA+TGG - chr4.1:15074541-15074560 None:intergenic 30.0%
!! CCAAAAACCTATCTTTTTCA+AGG + chr4.1:15072729-15072748 MS.gene09220:CDS 30.0%
!! TCTGCTTGTTATCTATGTTT+GGG + chr4.1:15073322-15073341 MS.gene09220:intron 30.0%
!! TTCTGCTTGTTATCTATGTT+TGG + chr4.1:15073321-15073340 MS.gene09220:intron 30.0%
!!! AACTTGCTGTCTTTTTTGAA+TGG + chr4.1:15073095-15073114 MS.gene09220:intron 30.0%
AAACTCTTACGCAAATAAGC+AGG - chr4.1:15073476-15073495 None:intergenic 35.0%
AAAGACAACACTAACACACA+AGG + chr4.1:15074278-15074297 MS.gene09220:CDS 35.0%
AACTTTCACATCACAATGCA+AGG + chr4.1:15073197-15073216 MS.gene09220:CDS 35.0%
AAGTCATTCCAAATCTCCTA+TGG + chr4.1:15072892-15072911 MS.gene09220:CDS 35.0%
ATACCATCTATAACCACCAT+AGG - chr4.1:15072911-15072930 None:intergenic 35.0%
CAACTAAGGTATGCATAACA+TGG - chr4.1:15073385-15073404 None:intergenic 35.0%
CAAGATCCCTTGAAAAAGAT+AGG - chr4.1:15072739-15072758 None:intergenic 35.0%
GATAATCTTTCGCTGATGAA+AGG - chr4.1:15072850-15072869 None:intergenic 35.0%
GTTAGTAAAGACTTCAGCTT+AGG - chr4.1:15073939-15073958 None:intergenic 35.0%
GTTTACCTTGCAAGTTACAA+TGG + chr4.1:15073492-15073511 MS.gene09220:intron 35.0%
TATAACCACCATAGGAGATT+TGG - chr4.1:15072903-15072922 None:intergenic 35.0%
TATATTAGAGTTGTGGGATG+AGG + chr4.1:15073785-15073804 MS.gene09220:intron 35.0%
TATGGTGGTTATAGATGGTA+TGG + chr4.1:15072910-15072929 MS.gene09220:CDS 35.0%
TCTTCTGTCAACTAATCCAT+GGG + chr4.1:15074497-15074516 MS.gene09220:CDS 35.0%
TGAGGACTGGATATAGTTAA+AGG + chr4.1:15073803-15073822 MS.gene09220:intron 35.0%
TTATTTGCTTCGAATGCTGA+AGG + chr4.1:15073125-15073144 MS.gene09220:intron 35.0%
! AATGTCCATTGTAACTTGCA+AGG - chr4.1:15073500-15073519 None:intergenic 35.0%
! CTTTTGTGTGGAAGAATGAT+TGG - chr4.1:15072421-15072440 None:intergenic 35.0%
!! AACATGACTGTTACTTTGCA+TGG - chr4.1:15073728-15073747 None:intergenic 35.0%
!! ACTTTTGGTTGCAAGAACTT+TGG - chr4.1:15074348-15074367 None:intergenic 35.0%
!! CAACCAAAAGTATCCAATTG+AGG + chr4.1:15074357-15074376 MS.gene09220:intron 35.0%
!!! ATATATTTTGGTTCCGCAGA+TGG + chr4.1:15074167-15074186 MS.gene09220:intron 35.0%
!!! ATATTTTTGGCTCTGCATCA+AGG + chr4.1:15072534-15072553 MS.gene09220:CDS 35.0%
!!! GATTTTCAGTTGCTTTTGTG+TGG - chr4.1:15072433-15072452 None:intergenic 35.0%
AGACTGCTACAAATTTAGCC+TGG + chr4.1:15074222-15074241 MS.gene09220:CDS 40.0%
CAAAATTACAACCACCAAGC+TGG + chr4.1:15072453-15072472 MS.gene09220:CDS 40.0%
CATACGTTTCGATGGTAAAG+AGG + chr4.1:15073634-15073653 MS.gene09220:intron 40.0%
CCAAAAATATCACTTCCACC+AGG - chr4.1:15072524-15072543 None:intergenic 40.0%
CTCTTCTGTCAACTAATCCA+TGG + chr4.1:15074496-15074515 MS.gene09220:CDS 40.0%
CTGACTCATTGCATGAGTTA+CGG - chr4.1:15074586-15074605 None:intergenic 40.0%
GCAGTCTCTGAATTAGCAAA+TGG - chr4.1:15074209-15074228 None:intergenic 40.0%
TCAACTGTCATACGTTTCGA+TGG + chr4.1:15073626-15073645 MS.gene09220:intron 40.0%
TCATTCCAAATCTCCTATGG+TGG + chr4.1:15072895-15072914 MS.gene09220:CDS 40.0%
TCTCCTATGGTGGTTATAGA+TGG + chr4.1:15072905-15072924 MS.gene09220:CDS 40.0%
TTAGCCTGGCAAAACATGAA+CGG + chr4.1:15074236-15074255 MS.gene09220:CDS 40.0%
TTAGTCGCATATGAATCCCA+TGG - chr4.1:15074516-15074535 None:intergenic 40.0%
! CCTGGTGGAAGTGATATTTT+TGG + chr4.1:15072521-15072540 MS.gene09220:CDS 40.0%
!! CAGCTTGGTGGTTGTAATTT+TGG - chr4.1:15072455-15072474 None:intergenic 40.0%
!! CTTTTGTTTGGAAGAGCTTG+CGG - chr4.1:15074262-15074281 None:intergenic 40.0%
AAGACCGAGAAATCAATGCC+GGG + chr4.1:15072486-15072505 MS.gene09220:CDS 45.0%
AATATCTCCCTCTTCCCAGT+GGG + chr4.1:15072385-15072404 MS.gene09220:CDS 45.0%
AGGTAGCGTACCCCTTATTA+AGG + chr4.1:15073858-15073877 MS.gene09220:intron 45.0%
CAAGACCGAGAAATCAATGC+CGG + chr4.1:15072485-15072504 MS.gene09220:CDS 45.0%
CATCGAACTCAAACAGATCG+TGG - chr4.1:15073153-15073172 None:intergenic 45.0%
CGGAAAACGAGTGTACATTG+AGG + chr4.1:15072685-15072704 MS.gene09220:CDS 45.0%
CTTTCGCTGATGAAAGGTCA+AGG - chr4.1:15072844-15072863 None:intergenic 45.0%
GAACCATTGCTCACTCTACT+CGG + chr4.1:15072665-15072684 MS.gene09220:CDS 45.0%
TTTCCGAGTAGAGTGAGCAA+TGG - chr4.1:15072671-15072690 None:intergenic 45.0%
! TCTTGTTTGATGCTCCAGCT+TGG - chr4.1:15072470-15072489 None:intergenic 45.0%
! TGACTTGATTGGAGTGCTTC+AGG - chr4.1:15073238-15073257 None:intergenic 45.0%
!! ACAACCCGGCATTGATTTCT+CGG - chr4.1:15072493-15072512 None:intergenic 45.0%
!! CATCTGTTGCTTTCCATCTG+CGG - chr4.1:15074183-15074202 None:intergenic 45.0%
!! TCAGTTTTGTCTTCACGCTG+TGG + chr4.1:15073259-15073278 MS.gene09220:CDS 45.0%
ACCAGGTGTATCGAACAACC+CGG - chr4.1:15072507-15072526 None:intergenic 50.0%
AGCTTTACCTGCCACATTGC+TGG - chr4.1:15072949-15072968 None:intergenic 50.0%
AGTTACGGCTTGAACAGCAC+TGG - chr4.1:15074571-15074590 None:intergenic 50.0%
CAATATCTCCCTCTTCCCAG+TGG + chr4.1:15072384-15072403 MS.gene09220:CDS 50.0%
CCAGAGACAGAAGAAAGCTC+TGG - chr4.1:15072644-15072663 None:intergenic 50.0%
CCAGAGCTTTCTTCTGTCTC+TGG + chr4.1:15072641-15072660 MS.gene09220:CDS 50.0%
TAGAGTTGTGGGATGAGGAC+TGG + chr4.1:15073790-15073809 MS.gene09220:intron 50.0%
TGTTTGATGCTCCAGCTTGG+TGG - chr4.1:15072467-15072486 None:intergenic 50.0%
! GATTTTGCCAGCAATGTGGC+AGG + chr4.1:15072939-15072958 MS.gene09220:CDS 50.0%
!!! CGTCGATTTTGCCAGCAATG+TGG + chr4.1:15072935-15072954 MS.gene09220:CDS 50.0%
CAAGGCCACAACCTTCAACC+TGG - chr4.1:15072826-15072845 None:intergenic 55.0%
GGGTTGTTCGATACACCTGG+TGG + chr4.1:15072506-15072525 MS.gene09220:CDS 55.0%
!! ACGGCTTGAACAGCACTGGT+TGG - chr4.1:15074567-15074586 None:intergenic 55.0%
CAACCTTCAACCTGGCAGCG+CGG - chr4.1:15072818-15072837 None:intergenic 60.0%
CTTGCAATTTCCGCGCTGCC+AGG + chr4.1:15072805-15072824 MS.gene09220:CDS 60.0%
GCCGGGTTGTTCGATACACC+TGG + chr4.1:15072503-15072522 MS.gene09220:CDS 60.0%
TCCCAGTCCCACTGGGAAGA+GGG - chr4.1:15072395-15072414 None:intergenic 60.0%
TCCCTCTTCCCAGTGGGACT+GGG + chr4.1:15072391-15072410 MS.gene09220:CDS 60.0%
! ATTGGTGCTCCCAGTCCCAC+TGG - chr4.1:15072403-15072422 None:intergenic 60.0%
! CGCTGCCAGGTTGAAGGTTG+TGG + chr4.1:15072818-15072837 MS.gene09220:CDS 60.0%
! GCGGCCGTTCATGTTTTGCC+AGG - chr4.1:15074243-15074262 None:intergenic 60.0%
! TTGGTGCTCCCAGTCCCACT+GGG - chr4.1:15072402-15072421 None:intergenic 60.0%
! TTTCCGCGCTGCCAGGTTGA+AGG + chr4.1:15072812-15072831 MS.gene09220:CDS 60.0%
CTCCCAGTCCCACTGGGAAG+AGG - chr4.1:15072396-15072415 None:intergenic 65.0%
CTCCCTCTTCCCAGTGGGAC+TGG + chr4.1:15072390-15072409 MS.gene09220:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 15072371 15074623 15072371 ID=MS.gene09220
chr4.1 mRNA 15072371 15074623 15072371 ID=MS.gene09220.t1;Parent=MS.gene09220
chr4.1 exon 15072371 15072960 15072371 ID=MS.gene09220.t1.exon1;Parent=MS.gene09220.t1
chr4.1 CDS 15072371 15072960 15072371 ID=cds.MS.gene09220.t1;Parent=MS.gene09220.t1
chr4.1 exon 15073147 15073280 15073147 ID=MS.gene09220.t1.exon2;Parent=MS.gene09220.t1
chr4.1 CDS 15073147 15073280 15073147 ID=cds.MS.gene09220.t1;Parent=MS.gene09220.t1
chr4.1 exon 15074186 15074299 15074186 ID=MS.gene09220.t1.exon3;Parent=MS.gene09220.t1
chr4.1 CDS 15074186 15074299 15074186 ID=cds.MS.gene09220.t1;Parent=MS.gene09220.t1
chr4.1 exon 15074433 15074623 15074433 ID=MS.gene09220.t1.exon4;Parent=MS.gene09220.t1
chr4.1 CDS 15074433 15074623 15074433 ID=cds.MS.gene09220.t1;Parent=MS.gene09220.t1
Gene Sequence

>MS.gene09220

ATGGACTTGAATGCAATATCTCCCTCTTCCCAGTGGGACTGGGAGCACCAATCATTCTTCCACACAAAAGCAACTGAAAATCCAAAATTACAACCACCAAGCTGGAGCATCAAACAAGACCGAGAAATCAATGCCGGGTTGTTCGATACACCTGGTGGAAGTGATATTTTTGGCTCTGCATCAAGGAGTTCAAAATCAGCTTCAAATAGTTCATCGTCAAATAGGGATAATAGCAAAACATGTATTTTTGCTTGTGGAAATTCTCATACACCAGAGCTTTCTTCTGTCTCTGGTGAACCATTGCTCACTCTACTCGGAAAACGAGTGTACATTGAGGATGTATGTCCGAAAAGTAATTCCAAAAACCTATCTTTTTCAAGGGATCTTGAGTCATCTTTAAGTATTGGAAAGAAATGTAAGTTCAATAGTCAGAACTTGCAATTTCCGCGCTGCCAGGTTGAAGGTTGTGGCCTTGACCTTTCATCAGCGAAAGATTATCATCGCAAACGAAGAGTTTGTGAAAGTCATTCCAAATCTCCTATGGTGGTTATAGATGGTATGGAACGTCGATTTTGCCAGCAATGTGGCAGGTAAAGCTTCTTAACAAATGTATGATTGTTTCAATGTAATTGACATAGGAAATAATTTTTTTATTTTTTTTTTGGTAGTGACGAAATGTTGTTAGCACATCATTATAAGCTAATATCACTACTCAGTCTGATTAAACTTGCTGTCTTTTTTGAATGGTGTTGAATTATTTGCTTCGAATGCTGAAGGTTCCACGATCTGTTTGAGTTCGATGAAAAGAAAAGAAGCTGCAGAGAACAACTTTCACATCACAATGCAAGGCGACGTAAGCATCAGCCTGAAGCACTCCAATCAAGTCAATCAGTTTTGTCTTCACGCTGTGGTAATACTTATTTTTCAATAGTTTTGCAGTTTATCTCAGTTTCTGCTTGTTATCTATGTTTGGGTGTTAAAGCTGAAAAGATGAAATGTGGAAACACAAGACCATGTTATGCATACCTTAGTTGACAATAAACATTGCTGAATGAAAGATGTTTTAAAACTTGACATGCTTAACGTCACATTTCTTTGCTGTCCTGCTTATTTGCGTAAGAGTTTACCTTGCAAGTTACAATGGACATTAAATGTGATGTGACAGAACTTCTTAATTTGCTACAATTTCCTATCTTTTTAAGCTCTTGTTAGTTCTGCTCATTTTTTCTTTAGTTTCATAGATCTTATTGGATTGTCAACTGTCATACGTTTCGATGGTAAAGAGGCTTAATCTTCTGTAACATGAATAAGTTAAGCATGTTCAAGAGTTCTAGCTGTAACAATTTTATAATAACCATGCAAAGTAACAGTCATGTTATTCTTGATAATTACTTATTGTCAGAAAGGATATTGCTATATTAGAGTTGTGGGATGAGGACTGGATATAGTTAAAGGCAGATTTAAGAGCCTAAGTCTTTAGAGAATTAAGGTAGCGTACCCCTTATTAAGGCATATGATACAAATAATTATCATGATTGTTTGGACTTGAGTGTGATTATGACATGCCTAAGCTGAAGTCTTTACTAACTAGATTTGTATGAGTATGCCATTCTTTAAAACCTGATTTTGTAAAAAAAACAACTTTAAATTTTGCTGTTAGTTGAAACATAGACCAAAGAACATTCACTTTTAGTTTTTTATAGGTTTATTGTAGTTTTTTTATAGGTTTATTGTAGTTAATTGCAAGTCGTTTATTTCTCAATGATTCAAATTTTACTGCTCTCTTCTAACTTCTAATATATTTTGGTTCCGCAGATGGAAAGCAACAGATGAGTCCATTTGCTAATTCAGAGACTGCTACAAATTTAGCCTGGCAAAACATGAACGGCCGCAAGCTCTTCCAAACAAAAGACAACACTAACACACAAGGTACTGTCACTATGCTTTCTGATGATTCCGTTGTCCATTTTACATCCAAAGTTCTTGCAACCAAAAGTATCCAATTGAGGTTATTTCACGTTTATTGCCACATTTTTTGTGCTGATTTTTTATAACTTTGCAGGTGTAGAAGATTTCATTACCGTTTCTGATACAAATGATAAACAAGACTTTACTTGTGCTCTCTCTCTTCTGTCAACTAATCCATGGGATTCATATGCGACTAAATCCATGTCTCTAGAACATTCTAATCGTCCAACCAGTGCTGTTCAAGCCGTAACTCATGCAATGAGTCAGCGCGTGCCTCTTGTTTAA
Protein sequence

>MS.gene09220.t1

MDLNAISPSSQWDWEHQSFFHTKATENPKLQPPSWSIKQDREINAGLFDTPGGSDIFGSASRSSKSASNSSSSNRDNSKTCIFACGNSHTPELSSVSGEPLLTLLGKRVYIEDVCPKSNSKNLSFSRDLESSLSIGKKCKFNSQNLQFPRCQVEGCGLDLSSAKDYHRKRRVCESHSKSPMVVIDGMERRFCQQCGRFHDLFEFDEKKRSCREQLSHHNARRRKHQPEALQSSQSVLSSRCDGKQQMSPFANSETATNLAWQNMNGRKLFQTKDNTNTQGVEDFITVSDTNDKQDFTCALSLLSTNPWDSYATKSMSLEHSNRPTSAVQAVTHAMSQRVPLV