AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene09424

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09424.t1 MTR_1g047060 54.000 100 37 3 110 207 1 93 2.76e-27 101
MS.gene09424.t1 MTR_4g101680 33.758 157 81 6 4 146 7 154 2.54e-17 80.5
MS.gene09424.t1 MTR_4g094302 29.508 183 91 7 4 172 11 169 3.68e-17 77.4
MS.gene09424.t1 MTR_5g021710 33.117 154 86 5 4 146 8 155 8.72e-17 79.0
MS.gene09424.t1 MTR_1g069805 34.641 153 82 7 4 146 10 154 3.13e-16 76.6
MS.gene09424.t1 MTR_7g100990 33.750 160 80 7 4 145 11 162 5.90e-16 76.3
MS.gene09424.t1 MTR_1g008740 33.758 157 86 6 2 146 25 175 9.48e-16 75.9
MS.gene09424.t1 MTR_2g080010 31.737 167 83 7 4 146 15 174 1.22e-15 75.5
MS.gene09424.t1 MTR_1g008740 33.758 157 86 6 2 146 11 161 1.23e-15 75.5
MS.gene09424.t1 MTR_8g076110 34.194 155 83 6 4 146 6 153 1.46e-15 75.1
MS.gene09424.t1 MTR_4g094302 33.333 129 77 4 4 132 11 130 1.91e-15 72.0
MS.gene09424.t1 MTR_5g041940 30.625 160 85 6 4 146 3 153 2.50e-15 73.6
MS.gene09424.t1 MTR_8g023840 34.416 154 80 8 4 145 11 155 3.06e-15 74.7
MS.gene09424.t1 MTR_4g089135 35.556 135 74 5 4 132 15 142 4.16e-15 73.9
MS.gene09424.t1 MTR_2g093810 31.875 160 83 6 4 145 14 165 4.91e-15 73.6
MS.gene09424.t1 MTR_4g035590 29.798 198 112 10 4 188 8 191 5.52e-15 73.9
MS.gene09424.t1 MTR_3g109340 33.121 157 86 6 2 146 20 169 6.71e-15 73.6
MS.gene09424.t1 MTR_3g093040 34.161 161 76 10 4 146 108 256 7.06e-15 73.2
MS.gene09424.t1 MTR_3g093050 33.742 163 80 10 1 146 19 170 1.24e-14 73.2
MS.gene09424.t1 MTR_8g023900 33.544 158 82 8 2 145 10 158 1.70e-14 72.8
MS.gene09424.t1 MTR_8g023900 29.255 188 108 8 7 180 196 372 1.34e-11 64.3
MS.gene09424.t1 MTR_3g093050 34.375 160 77 10 4 146 22 170 1.91e-14 72.8
MS.gene09424.t1 MTR_8g467490 25.630 238 132 9 2 203 12 240 2.54e-14 72.0
MS.gene09424.t1 MTR_8g023860 31.410 156 88 6 1 145 9 156 4.84e-14 71.2
MS.gene09424.t1 MTR_3g064580 33.962 159 80 9 4 146 6 155 4.87e-14 71.6
MS.gene09424.t1 MTR_1g090720 34.356 163 79 7 4 146 42 196 1.31e-13 70.1
MS.gene09424.t1 MTR_5g014300 30.769 195 109 10 4 180 6 192 1.47e-13 69.7
MS.gene09424.t1 MTR_6g011860 31.515 165 83 9 4 146 15 171 3.18e-13 68.9
MS.gene09424.t1 MTR_5g069030 34.161 161 76 10 4 146 25 173 3.70e-13 68.9
MS.gene09424.t1 MTR_3g116070 31.293 147 89 5 4 144 23 163 4.39e-13 68.2
MS.gene09424.t1 MTR_7g005280 31.613 155 83 7 4 146 15 158 8.07e-13 66.6
MS.gene09424.t1 MTR_5g076850 32.704 159 81 9 4 146 6 154 8.34e-13 68.2
MS.gene09424.t1 MTR_8g024480 27.381 168 88 7 4 146 15 173 1.85e-12 66.6
MS.gene09424.t1 MTR_6g084430 32.051 156 86 7 2 146 3 149 2.71e-12 65.9
MS.gene09424.t1 MTR_2g079990 35.294 136 71 7 4 132 16 141 2.85e-12 65.9
MS.gene09424.t1 MTR_7g085220 28.571 168 87 8 4 146 8 167 7.60e-12 64.7
MS.gene09424.t1 MTR_2g078700 30.818 159 86 6 4 146 23 173 1.50e-11 63.9
MS.gene09424.t1 MTR_3g096400 29.518 166 93 5 4 152 7 165 3.04e-11 61.6
MS.gene09424.t1 MTR_3g070030 28.824 170 93 7 4 154 19 179 9.29e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09424.t1 AT4G36160 33.544 158 88 5 4 150 10 161 1.03e-18 84.7
MS.gene09424.t1 AT4G36160 33.544 158 88 5 4 150 10 161 1.03e-18 84.7
MS.gene09424.t1 AT4G36160 33.544 158 88 5 4 150 14 165 1.17e-18 84.3
MS.gene09424.t1 AT1G71930 33.121 157 88 5 4 149 9 159 6.78e-18 81.6
MS.gene09424.t1 AT1G71930 33.121 157 88 5 4 149 9 159 6.78e-18 81.6
MS.gene09424.t1 AT2G24430 35.669 157 82 6 2 146 14 163 1.54e-17 80.9
MS.gene09424.t1 AT2G24430 35.669 157 82 6 2 146 14 163 1.54e-17 80.9
MS.gene09424.t1 AT2G18060 33.117 154 86 5 4 146 9 156 2.98e-17 80.5
MS.gene09424.t1 AT2G18060 33.117 154 86 5 4 146 9 156 2.98e-17 80.5
MS.gene09424.t1 AT4G28530 33.133 166 85 5 4 147 10 171 3.34e-16 77.4
MS.gene09424.t1 AT1G69490 32.500 160 82 7 4 146 9 159 6.17e-16 75.5
MS.gene09424.t1 AT1G26870 33.974 156 86 6 2 149 14 160 7.19e-16 76.6
MS.gene09424.t1 AT3G10490 33.544 158 82 7 4 146 27 176 1.86e-15 73.9
MS.gene09424.t1 AT4G10350 33.962 159 80 9 4 146 9 158 3.96e-15 74.3
MS.gene09424.t1 AT2G27300 29.452 146 93 4 4 145 14 153 4.11e-15 73.9
MS.gene09424.t1 AT4G17980 33.333 159 82 7 4 145 6 157 4.65e-15 73.2
MS.gene09424.t1 AT4G17980 33.125 160 83 7 3 145 5 157 5.82e-15 73.2
MS.gene09424.t1 AT5G63790 33.333 162 80 7 4 147 50 201 1.15e-14 72.4
MS.gene09424.t1 AT5G63790 33.333 162 80 7 4 147 60 211 1.35e-14 72.4
MS.gene09424.t1 AT3G03200 34.375 160 78 9 4 146 6 155 1.51e-14 73.2
MS.gene09424.t1 AT1G54330 32.026 153 87 6 4 146 6 151 1.86e-14 72.0
MS.gene09424.t1 AT5G64530 33.588 131 74 4 4 134 3 120 2.61e-14 69.7
MS.gene09424.t1 AT5G64530 33.588 131 74 4 4 134 3 120 2.94e-14 68.9
MS.gene09424.t1 AT3G10480 33.121 157 83 7 4 146 27 175 5.06e-14 71.2
MS.gene09424.t1 AT4G35580 31.902 163 89 8 1 149 6 160 6.73e-14 71.2
MS.gene09424.t1 AT4G35580 31.902 163 89 8 1 149 6 160 6.79e-14 71.2
MS.gene09424.t1 AT4G35580 31.902 163 89 8 1 149 6 160 8.03e-14 70.9
MS.gene09424.t1 AT5G39610 32.278 158 83 7 4 146 20 168 1.16e-13 69.3
MS.gene09424.t1 AT1G61110 34.058 138 75 6 4 134 16 144 1.80e-13 69.3
MS.gene09424.t1 AT5G04410 31.646 158 84 6 4 146 9 157 2.25e-13 69.7
MS.gene09424.t1 AT5G39820 33.835 133 75 5 6 132 22 147 3.29e-13 68.6
MS.gene09424.t1 AT1G32770 28.729 181 85 8 4 149 16 187 4.83e-13 68.2
MS.gene09424.t1 AT3G10500 32.278 158 83 7 4 146 9 157 4.87e-13 68.6
MS.gene09424.t1 AT2G46770 26.368 201 107 8 4 175 16 204 7.18e-13 67.8
MS.gene09424.t1 AT3G15170 31.447 159 85 7 3 145 19 169 7.53e-13 67.4
MS.gene09424.t1 AT3G18400 31.646 158 87 8 2 147 3 151 1.04e-12 67.0
MS.gene09424.t1 AT3G29035 31.447 159 83 7 4 146 24 172 1.34e-12 66.6
MS.gene09424.t1 AT4G27410 29.240 171 86 7 4 146 14 177 1.77e-12 66.2
MS.gene09424.t1 AT5G61430 32.278 158 83 7 4 146 16 164 2.21e-12 66.2
MS.gene09424.t1 AT3G15510 29.814 161 82 7 10 146 23 176 2.36e-12 66.2
MS.gene09424.t1 AT5G22290 28.571 147 90 6 6 145 23 161 2.62e-12 65.9
MS.gene09424.t1 AT5G24590 30.380 158 87 7 4 146 13 162 4.15e-12 65.9
MS.gene09424.t1 AT2G17040 30.323 155 82 7 6 146 8 150 4.22e-12 65.1
MS.gene09424.t1 AT1G32870 32.331 133 79 5 4 132 10 135 8.11e-12 65.1
MS.gene09424.t1 AT3G04060 33.333 159 81 9 4 146 20 169 8.59e-12 64.3
MS.gene09424.t1 AT1G32870 32.331 133 79 5 4 132 10 135 9.02e-12 64.7
MS.gene09424.t1 AT1G32870 32.331 133 79 5 4 132 46 171 1.30e-11 64.3
MS.gene09424.t1 AT5G53950 29.814 161 89 6 2 146 15 167 2.13e-11 63.5
MS.gene09424.t1 AT5G46590 32.374 139 80 6 4 135 6 137 2.33e-11 62.8
MS.gene09424.t1 AT5G04395 31.333 150 80 6 4 135 28 172 4.14e-11 60.8

Find 49 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCAACTAATGCTTTCAATTA+TGG 0.184656 6.4:+22591887 MS.gene09424:CDS
CAATGGTTCTGACTTGATTA+AGG 0.258416 6.4:+22589680 MS.gene09424:CDS
GGCAATGTAAGATTGGATTT+TGG 0.279853 6.4:+22589814 MS.gene09424:CDS
CCTTGGGTGACGGAAGAAAA+AGG 0.350861 6.4:-22589751 None:intergenic
ATTGAAAGCATTAGTTGCTC+TGG 0.380713 6.4:-22591882 None:intergenic
ATGGATCTGGATGTTGAGTT+TGG 0.395501 6.4:+22590142 MS.gene09424:CDS
CCTCATCTGCTCATCAACTT+TGG 0.414860 6.4:-22589793 None:intergenic
GAAGAGGTTCAAAATTTCAA+TGG 0.434760 6.4:+22589663 MS.gene09424:CDS
GGAGTAAGACGCAATTCAAA+TGG 0.441267 6.4:-22589726 None:intergenic
GCTGTTCGAGACATTATTGT+TGG 0.457958 6.4:+22589849 MS.gene09424:CDS
GCAATTCAAATGGGTCATAC+TGG 0.459319 6.4:-22589716 None:intergenic
CAAATGGGTCATACTGGTAC+AGG 0.462094 6.4:-22589710 None:intergenic
CAGATGAGGCAATGTAAGAT+TGG 0.462721 6.4:+22589807 MS.gene09424:CDS
TTGAAAGCATTAGTTGCTCT+GGG 0.473709 6.4:-22591881 None:intergenic
CGAAGTTACTCAGAAACCTA+TGG 0.482750 6.4:+22590219 MS.gene09424:CDS
GAATTGCTTCCACCTGGTGT+TGG 0.483908 6.4:+22589585 MS.gene09424:CDS
AACACACGACAAAGAACAAA+AGG 0.484780 6.4:-22590103 None:intergenic
TCAATTAATTTACACAAGAT+CGG 0.501330 6.4:-22591931 None:intergenic
GTGAAGCAGTAAAATTCCCT+TGG 0.511128 6.4:-22589768 None:intergenic
AGAATGGCATCAAAACTAGT+TGG 0.512078 6.4:+22589934 MS.gene09424:CDS
CTGCCCACCAGAGATGGATC+TGG 0.513629 6.4:+22590129 MS.gene09424:CDS
TCAACATCCAGATCCATCTC+TGG 0.528153 6.4:-22590136 None:intergenic
CACTCTTGCTAACAATCCTC+AGG 0.528444 6.4:+22589971 MS.gene09424:CDS
GTTGATGAAGGTGATGCTAC+TGG 0.539280 6.4:+22590172 MS.gene09424:CDS
AGTAAAATTCCCTTGGGTGA+CGG 0.539767 6.4:-22589761 None:intergenic
GAAGGTGATGCTACTGGAGA+TGG 0.546862 6.4:+22590178 MS.gene09424:CDS
GAGTAAGACGCAATTCAAAT+GGG 0.549774 6.4:-22589725 None:intergenic
TGAAGCAGTAAAATTCCCTT+GGG 0.559532 6.4:-22589767 None:intergenic
CCAAAGTTGATGAGCAGATG+AGG 0.560720 6.4:+22589793 MS.gene09424:CDS
ACATCCAGATCCATCTCTGG+TGG 0.568257 6.4:-22590133 None:intergenic
GAAGTTACTCAGAAACCTAT+GGG 0.580237 6.4:+22590220 MS.gene09424:CDS
GAAGGGATGAAGCCAACACC+AGG 0.588550 6.4:-22589597 None:intergenic
TTTGGAGCAGATGTTGATGA+AGG 0.590115 6.4:+22590160 MS.gene09424:CDS
ATAGGAAATTCCGCTCAGAA+TGG 0.595921 6.4:+22589918 MS.gene09424:CDS
GTTTGACTGCCCACCAGAGA+TGG 0.602762 6.4:+22590123 MS.gene09424:CDS
TGTTGGAACTGATAACGTGT+TGG 0.614058 6.4:+22589866 MS.gene09424:CDS
TAACAAGGAGTTCATCAGAA+GGG 0.616296 6.4:-22589614 None:intergenic
GCAATGGAATTGCTTCCACC+TGG 0.624048 6.4:+22589579 None:intergenic
TGGAACTGATAACGTGTTGG+TGG 0.634013 6.4:+22589869 MS.gene09424:CDS
TGACGATGTTGTCTTGCACA+TGG 0.636821 6.4:+22591848 MS.gene09424:CDS
TGGCAAGGTAGTAGATAACA+AGG 0.641793 6.4:-22589629 None:intergenic
ATAACAAGGAGTTCATCAGA+AGG 0.650392 6.4:-22589615 None:intergenic
CATCCAGATCCATCTCTGGT+GGG 0.656418 6.4:-22590132 None:intergenic
GGGATGAAGCCAACACCAGG+TGG 0.665656 6.4:-22589594 None:intergenic
GGAACTGATAACGTGTTGGT+GGG 0.675682 6.4:+22589870 MS.gene09424:CDS
AAGTTACTCAGAAACCTATG+GGG 0.699471 6.4:+22590221 MS.gene09424:CDS
AGTGCTGCTGTTGAGCAACG+CGG 0.701822 6.4:+22590265 MS.gene09424:CDS
TGAAAGCATTAGTTGCTCTG+GGG 0.714933 6.4:-22591880 None:intergenic
AGTTACTCAGAAACCTATGG+GGG 0.738835 6.4:+22590222 MS.gene09424:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAAAATTAAATTGTAATG+AGG - chr6.4:22591231-22591250 None:intergenic 10.0%
!! TATTATTATTATTATGAGTT+AGG + chr6.4:22590725-22590744 MS.gene09424:intron 10.0%
!!! TTTTTTTAAAATTATTATGC+AGG + chr6.4:22590078-22590097 MS.gene09424:intron 10.0%
!! ATAATAATAATAATAGAGTC+AGG - chr6.4:22591610-22591629 None:intergenic 15.0%
!! ATATGAATTAAAATAATCCT+AGG + chr6.4:22591140-22591159 MS.gene09424:intron 15.0%
!! CTTTGATTAAATAAAATCAA+CGG + chr6.4:22591537-22591556 MS.gene09424:intron 15.0%
!!! GTATTTTAATTATTCAGATA+TGG + chr6.4:22591367-22591386 MS.gene09424:intron 15.0%
!!! TTTTAATTCATATTCAATCA+TGG - chr6.4:22591133-22591152 None:intergenic 15.0%
!! AAAAAAATACATCACATGAA+TGG - chr6.4:22590431-22590450 None:intergenic 20.0%
!! AATACATTAAATAATCATCG+AGG - chr6.4:22590039-22590058 None:intergenic 20.0%
!! AATATAAAGAGAGATCAAAA+TGG - chr6.4:22590340-22590359 None:intergenic 20.0%
!! AGAAGAAGAATAATATTCAT+AGG - chr6.4:22590952-22590971 None:intergenic 20.0%
!! ATAAGATCAACAACATTAAT+TGG - chr6.4:22590365-22590384 None:intergenic 20.0%
!! CTTATGATAACAATTACAAT+TGG + chr6.4:22591041-22591060 MS.gene09424:intron 20.0%
!! TGATAATTCTCAAAACATAT+AGG + chr6.4:22590544-22590563 MS.gene09424:intron 20.0%
!!! AAAGAAACATGGATTAATTA+TGG - chr6.4:22590798-22590817 None:intergenic 20.0%
!!! AAGAAACATGGATTAATTAT+GGG - chr6.4:22590797-22590816 None:intergenic 20.0%
!!! CAACTTTTCATTTCATATTA+TGG + chr6.4:22591744-22591763 MS.gene09424:intron 20.0%
!!! CGTTGATTTTATTTAATCAA+AGG - chr6.4:22591539-22591558 None:intergenic 20.0%
!!! TCTCTTTTTATGTTCTATAT+AGG + chr6.4:22589900-22589919 MS.gene09424:CDS 20.0%
! AAAAACTCAAACTGAAAACA+TGG + chr6.4:22590871-22590890 MS.gene09424:intron 25.0%
! AAGAATAATATTCATAGGAG+TGG - chr6.4:22590947-22590966 None:intergenic 25.0%
! AATACTTCAACAATACATTC+TGG - chr6.4:22591776-22591795 None:intergenic 25.0%
! AGAACAAAGAGAATTATGAT+AGG + chr6.4:22591413-22591432 MS.gene09424:intron 25.0%
! AGAATAATATTCATAGGAGT+GGG - chr6.4:22590946-22590965 None:intergenic 25.0%
! ATACACAAGAAAAAGAAACA+TGG - chr6.4:22590809-22590828 None:intergenic 25.0%
! ATAGGATTATGTTTCCAATT+TGG + chr6.4:22591431-22591450 MS.gene09424:intron 25.0%
! ATAGTGTGTGATTGAAAAAA+AGG + chr6.4:22591478-22591497 MS.gene09424:intron 25.0%
! TAAAAGCAATAATTGTGTGT+GGG - chr6.4:22590843-22590862 None:intergenic 25.0%
! TACATTTAGTAGCAAAGATT+GGG - chr6.4:22590459-22590478 None:intergenic 25.0%
! TAGGATTATGTTTCCAATTT+GGG + chr6.4:22591432-22591451 MS.gene09424:intron 25.0%
! TTACATTTAGTAGCAAAGAT+TGG - chr6.4:22590460-22590479 None:intergenic 25.0%
!! GTTTAAAACGATAAAATCCT+AGG - chr6.4:22591160-22591179 None:intergenic 25.0%
!! TCAAATTTCACAGATTTTGA+AGG + chr6.4:22590292-22590311 MS.gene09424:CDS 25.0%
!!! AACCTCTTCATTATTTTTGT+TGG - chr6.4:22589652-22589671 None:intergenic 25.0%
AATAATATTCATAGGAGTGG+GGG - chr6.4:22590944-22590963 None:intergenic 30.0%
AATGATTACCAAACCCAAAT+TGG - chr6.4:22591448-22591467 None:intergenic 30.0%
ACATTTAGTAGCAAAGATTG+GGG - chr6.4:22590458-22590477 None:intergenic 30.0%
ATTACAATTGGAGAGGAATA+TGG + chr6.4:22591053-22591072 MS.gene09424:intron 30.0%
CTAAAAGCAATAATTGTGTG+TGG - chr6.4:22590844-22590863 None:intergenic 30.0%
GAAGAGGTTCAAAATTTCAA+TGG + chr6.4:22589663-22589682 MS.gene09424:CDS 30.0%
GAATAATATTCATAGGAGTG+GGG - chr6.4:22590945-22590964 None:intergenic 30.0%
GATAACAATTACAATTGGAG+AGG + chr6.4:22591046-22591065 MS.gene09424:intron 30.0%
GCAACTAATGCTTTCAATTA+TGG + chr6.4:22591887-22591906 MS.gene09424:CDS 30.0%
TAAAATCAACGGTTGAGATT+TGG + chr6.4:22591548-22591567 MS.gene09424:intron 30.0%
TGCCAACAAAAATAATGAAG+AGG + chr6.4:22589647-22589666 MS.gene09424:CDS 30.0%
TGTCTACAAAATGAATCGTA+TGG + chr6.4:22591187-22591206 MS.gene09424:intron 30.0%
! AATTGGTTCAATTAGCATGT+AGG + chr6.4:22591310-22591329 MS.gene09424:intron 30.0%
! ATACTCAACGATTCTCTTTT+GGG + chr6.4:22590901-22590920 MS.gene09424:intron 30.0%
! ATGGAATATATTTTACCTGC+AGG - chr6.4:22590321-22590340 None:intergenic 30.0%
! CAAAATGAATCGTATGGTTT+TGG + chr6.4:22591193-22591212 MS.gene09424:intron 30.0%
! TGGAATATATTTTACCTGCA+GGG - chr6.4:22590320-22590339 None:intergenic 30.0%
! TTATGTTTCCAATTTGGGTT+TGG + chr6.4:22591437-22591456 MS.gene09424:intron 30.0%
!! TGAATTGCTTTGCTTGTTTT+TGG + chr6.4:22591795-22591814 MS.gene09424:intron 30.0%
!!! CTTCATTATTTTTGTTGGCA+AGG - chr6.4:22589647-22589666 None:intergenic 30.0%
AACAAAGTCACCACAAGTTT+GGG + chr6.4:22591277-22591296 MS.gene09424:intron 35.0%
AACACACGACAAAGAACAAA+AGG - chr6.4:22590106-22590125 None:intergenic 35.0%
AAGAACTGACGAATAAGCTT+TGG - chr6.4:22590762-22590781 None:intergenic 35.0%
AAGTTACTCAGAAACCTATG+GGG + chr6.4:22590221-22590240 MS.gene09424:CDS 35.0%
AATCCTCAGGTATGGAATTT+GGG + chr6.4:22589984-22590003 MS.gene09424:intron 35.0%
AGAATGGCATCAAAACTAGT+TGG + chr6.4:22589934-22589953 MS.gene09424:CDS 35.0%
ATAACAAGGAGTTCATCAGA+AGG - chr6.4:22589618-22589637 None:intergenic 35.0%
ATACGAGGAGTTAAGTAAGA+GGG - chr6.4:22590988-22591007 None:intergenic 35.0%
GAAGTTACTCAGAAACCTAT+GGG + chr6.4:22590220-22590239 MS.gene09424:CDS 35.0%
GAGTAAGACGCAATTCAAAT+GGG - chr6.4:22589728-22589747 None:intergenic 35.0%
GTATGTATTGCAACACATGA+AGG + chr6.4:22591004-22591023 MS.gene09424:intron 35.0%
TAACAAGGAGTTCATCAGAA+GGG - chr6.4:22589617-22589636 None:intergenic 35.0%
TGAAGCAGTAAAATTCCCTT+GGG - chr6.4:22589770-22589789 None:intergenic 35.0%
! ATGGATTAATTATGGGCCTT+TGG - chr6.4:22590790-22590809 None:intergenic 35.0%
! ATTGGATTTTGGATGCAGAA+GGG + chr6.4:22589825-22589844 MS.gene09424:CDS 35.0%
! CGTCAGTTCTTTTAATCCAA+AGG + chr6.4:22590771-22590790 MS.gene09424:intron 35.0%
! GATACTCAACGATTCTCTTT+TGG + chr6.4:22590900-22590919 MS.gene09424:intron 35.0%
! GGCAATGTAAGATTGGATTT+TGG + chr6.4:22589814-22589833 MS.gene09424:CDS 35.0%
!! ATTGAAAGCATTAGTTGCTC+TGG - chr6.4:22591885-22591904 None:intergenic 35.0%
!! CAATGGTTCTGACTTGATTA+AGG + chr6.4:22589680-22589699 MS.gene09424:CDS 35.0%
!! TTGAAAGCATTAGTTGCTCT+GGG - chr6.4:22591884-22591903 None:intergenic 35.0%
!!! ATCGTATGGTTTTGGTAGAA+TGG + chr6.4:22591201-22591220 MS.gene09424:intron 35.0%
!!! GCTTGTTTTTGGTGTTTATC+AGG + chr6.4:22591806-22591825 MS.gene09424:intron 35.0%
AGGACGTGATTTGAAGAATC+TGG - chr6.4:22590600-22590619 None:intergenic 40.0%
AGGAGTTAAGTAAGAGGGAA+AGG - chr6.4:22590983-22591002 None:intergenic 40.0%
AGTAAAATTCCCTTGGGTGA+CGG - chr6.4:22589764-22589783 None:intergenic 40.0%
AGTTACTCAGAAACCTATGG+GGG + chr6.4:22590222-22590241 MS.gene09424:CDS 40.0%
ATAGGAAATTCCGCTCAGAA+TGG + chr6.4:22589918-22589937 MS.gene09424:CDS 40.0%
ATCGAGGAAATCACGAACAA+AGG - chr6.4:22590023-22590042 None:intergenic 40.0%
ATGGATCTGGATGTTGAGTT+TGG + chr6.4:22590142-22590161 MS.gene09424:CDS 40.0%
CAACAAAGTCACCACAAGTT+TGG + chr6.4:22591276-22591295 MS.gene09424:intron 40.0%
CAATCCTCAGGTATGGAATT+TGG + chr6.4:22589983-22590002 MS.gene09424:intron 40.0%
CAGATGAGGCAATGTAAGAT+TGG + chr6.4:22589807-22589826 MS.gene09424:CDS 40.0%
CATACGAGGAGTTAAGTAAG+AGG - chr6.4:22590989-22591008 None:intergenic 40.0%
CATGTGTTGCAATACATACG+AGG - chr6.4:22591003-22591022 None:intergenic 40.0%
CATTGTCAAAAAGTGACGAC+AGG - chr6.4:22591724-22591743 None:intergenic 40.0%
CGAAGTTACTCAGAAACCTA+TGG + chr6.4:22590219-22590238 MS.gene09424:CDS 40.0%
GAGAGGAATATGGTGAAAGT+AGG + chr6.4:22591063-22591082 MS.gene09424:intron 40.0%
GCAATTCAAATGGGTCATAC+TGG - chr6.4:22589719-22589738 None:intergenic 40.0%
GCTGTTCGAGACATTATTGT+TGG + chr6.4:22589849-22589868 MS.gene09424:CDS 40.0%
GGAGTAAGACGCAATTCAAA+TGG - chr6.4:22589729-22589748 None:intergenic 40.0%
GTGAAGCAGTAAAATTCCCT+TGG - chr6.4:22589771-22589790 None:intergenic 40.0%
TCGAGGAAATCACGAACAAA+GGG - chr6.4:22590022-22590041 None:intergenic 40.0%
TGGCAAGGTAGTAGATAACA+AGG - chr6.4:22589632-22589651 None:intergenic 40.0%
TTGCTAACAATCCTCAGGTA+TGG + chr6.4:22589976-22589995 MS.gene09424:intron 40.0%
TTTCCCAAATTCCATACCTG+AGG - chr6.4:22589990-22590009 None:intergenic 40.0%
! GATTGGATTTTGGATGCAGA+AGG + chr6.4:22589824-22589843 MS.gene09424:CDS 40.0%
! TGTTGGAACTGATAACGTGT+TGG + chr6.4:22589866-22589885 MS.gene09424:CDS 40.0%
! TTGGATTTTGGATGCAGAAG+GGG + chr6.4:22589826-22589845 MS.gene09424:CDS 40.0%
! TTTGGAGCAGATGTTGATGA+AGG + chr6.4:22590160-22590179 MS.gene09424:CDS 40.0%
!! AGTTTTGATGCCATTCTGAG+CGG - chr6.4:22589931-22589950 None:intergenic 40.0%
!! CGAATAAGCTTTGGTGTCAA+CGG - chr6.4:22590753-22590772 None:intergenic 40.0%
!! TGAAAGCATTAGTTGCTCTG+GGG - chr6.4:22591883-22591902 None:intergenic 40.0%
AAGTCACCACAAGTTTGGGA+AGG + chr6.4:22591281-22591300 MS.gene09424:intron 45.0%
AGTCACCACAAGTTTGGGAA+GGG + chr6.4:22591282-22591301 MS.gene09424:intron 45.0%
CAAATGGGTCATACTGGTAC+AGG - chr6.4:22589713-22589732 None:intergenic 45.0%
CACTCTTGCTAACAATCCTC+AGG + chr6.4:22589971-22589990 MS.gene09424:CDS 45.0%
CCTCATCTGCTCATCAACTT+TGG - chr6.4:22589796-22589815 None:intergenic 45.0%
CGAGGAAATCACGAACAAAG+GGG - chr6.4:22590021-22590040 None:intergenic 45.0%
GTTTGGGAAGGGTTGTGAAT+TGG + chr6.4:22591293-22591312 MS.gene09424:intron 45.0%
TCAACATCCAGATCCATCTC+TGG - chr6.4:22590139-22590158 None:intergenic 45.0%
TCAATGAATCCGTTGACACC+TGG + chr6.4:22591573-22591592 MS.gene09424:intron 45.0%
TGACGATGTTGTCTTGCACA+TGG + chr6.4:22591848-22591867 MS.gene09424:CDS 45.0%
! CTTTTTCTTCCGTCACCCAA+GGG + chr6.4:22589752-22589771 MS.gene09424:CDS 45.0%
! GGAACTGATAACGTGTTGGT+GGG + chr6.4:22589870-22589889 MS.gene09424:CDS 45.0%
! GTTGATGAAGGTGATGCTAC+TGG + chr6.4:22590172-22590191 MS.gene09424:CDS 45.0%
! TGGAACTGATAACGTGTTGG+TGG + chr6.4:22589869-22589888 MS.gene09424:CDS 45.0%
! TGGATTTTGGATGCAGAAGG+GGG + chr6.4:22589827-22589846 MS.gene09424:CDS 45.0%
!! CCAAAGTTGATGAGCAGATG+AGG + chr6.4:22589793-22589812 MS.gene09424:CDS 45.0%
!! AAATTTAAAGAAAAATAAAA+AGG - chr6.4:22590620-22590639 None:intergenic 5.0%
ACATCCAGATCCATCTCTGG+TGG - chr6.4:22590136-22590155 None:intergenic 50.0%
CACAACCCTTCCCAAACTTG+TGG - chr6.4:22591290-22591309 None:intergenic 50.0%
CATCCAGATCCATCTCTGGT+GGG - chr6.4:22590135-22590154 None:intergenic 50.0%
GAAGGTGATGCTACTGGAGA+TGG + chr6.4:22590178-22590197 MS.gene09424:CDS 50.0%
GAATTGCTTCCACCTGGTGT+TGG + chr6.4:22589585-22589604 MS.gene09424:CDS 50.0%
GGAAATCACGAACAAAGGGG+AGG - chr6.4:22590018-22590037 None:intergenic 50.0%
TCAGAAACCTATGGGGGCTA+GGG + chr6.4:22590228-22590247 MS.gene09424:CDS 50.0%
! CCTTTTTCTTCCGTCACCCA+AGG + chr6.4:22589751-22589770 MS.gene09424:CDS 50.0%
!! CCTTGGGTGACGGAAGAAAA+AGG - chr6.4:22589754-22589773 None:intergenic 50.0%
!! GGGTGACGGAAGAAAAAGGA+CGG - chr6.4:22589750-22589769 None:intergenic 50.0%
!!! AGATTTTGAAGGCACCCTGC+AGG + chr6.4:22590303-22590322 MS.gene09424:CDS 50.0%
AAACCTATGGGGGCTAGGGA+TGG + chr6.4:22590232-22590251 MS.gene09424:CDS 55.0%
AACCTATGGGGGCTAGGGAT+GGG + chr6.4:22590233-22590252 MS.gene09424:CDS 55.0%
AGTCAGGATCCGTTGACACC+AGG - chr6.4:22591594-22591613 None:intergenic 55.0%
CCGTTGACACCAGGTGTCAA+CGG - chr6.4:22591585-22591604 None:intergenic 55.0%
CTCAGAAACCTATGGGGGCT+AGG + chr6.4:22590227-22590246 MS.gene09424:CDS 55.0%
GAAGGGATGAAGCCAACACC+AGG - chr6.4:22589600-22589619 None:intergenic 55.0%
GTTTGACTGCCCACCAGAGA+TGG + chr6.4:22590123-22590142 MS.gene09424:CDS 55.0%
! AGTGCTGCTGTTGAGCAACG+CGG + chr6.4:22590265-22590284 MS.gene09424:CDS 55.0%
! CCGTTGACACCTGGTGTCAA+CGG + chr6.4:22591582-22591601 MS.gene09424:intron 55.0%
ACCTATGGGGGCTAGGGATG+GGG + chr6.4:22590234-22590253 MS.gene09424:CDS 60.0%
CTGCCCACCAGAGATGGATC+TGG + chr6.4:22590129-22590148 MS.gene09424:CDS 60.0%
GGGATGAAGCCAACACCAGG+TGG - chr6.4:22589597-22589616 None:intergenic 60.0%
ATGGGGGCTAGGGATGGGGC+TGG + chr6.4:22590238-22590257 MS.gene09424:CDS 70.0%
GCCCCATCCCTAGCCCCCAT+AGG - chr6.4:22590238-22590257 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr6.4 gene 22589582 22591944 22589582 ID=MS.gene09424
chr6.4 mRNA 22589582 22591944 22589582 ID=MS.gene09424.t1;Parent=MS.gene09424
chr6.4 exon 22589582 22589992 22589582 ID=MS.gene09424.t1.exon1;Parent=MS.gene09424.t1
chr6.4 CDS 22589582 22589992 22589582 ID=cds.MS.gene09424.t1;Parent=MS.gene09424.t1
chr6.4 exon 22590100 22590324 22590100 ID=MS.gene09424.t1.exon2;Parent=MS.gene09424.t1
chr6.4 CDS 22590100 22590324 22590100 ID=cds.MS.gene09424.t1;Parent=MS.gene09424.t1
chr6.4 exon 22591828 22591944 22591828 ID=MS.gene09424.t1.exon3;Parent=MS.gene09424.t1
chr6.4 CDS 22591828 22591944 22591828 ID=cds.MS.gene09424.t1;Parent=MS.gene09424.t1
Gene Sequence

>MS.gene09424

ATGGAATTGCTTCCACCTGGTGTTGGCTTCATCCCTTCTGATGAACTCCTTGTTATCTACTACCTTGCCAACAAAAATAATGAAGAGGTTCAAAATTTCAATGGTTCTGACTTGATTAAGGAGCTAAACCTGTACCAGTATGACCCATTTGAATTGCGTCTTACTCCGTCCTTTTTCTTCCGTCACCCAAGGGAATTTTACTGCTTCACTGCCAAAGTTGATGAGCAGATGAGGCAATGTAAGATTGGATTTTGGATGCAGAAGGGGGCTGTTCGAGACATTATTGTTGGAACTGATAACGTGTTGGTGGGAAAGAAGTCTCTTTTTATGTTCTATATAGGAAATTCCGCTCAGAATGGCATCAAAACTAGTTGGACTATGTACGAGTACACTCTTGCTAACAATCCTCAGGTATGGAATTTGGGAAAACTAACCTCCCCTTTGTTCGTGATTTCCTCGATGATTATTTAATGTATTAGCAACTACTAATTGTTTTTTTTTTTAAAATTATTATGCAGGCTCCTTTTGTTCTTTGTCGTGTGTTTGACTGCCCACCAGAGATGGATCTGGATGTTGAGTTTGGAGCAGATGTTGATGAAGGTGATGCTACTGGAGATGGCTCTGCTAACAAGCTGAACGAAGTTACTCAGAAACCTATGGGGGCTAGGGATGGGGCTGGTACTAGTGCTGCTGTTGAGCAACGCGGTGCATCAAATTTCACAGATTTTGAAGGCACCCTGCAGGTAAAATATATTCCATTTTGATCTCTCTTTATATTAACCAATTAATGTTGTTGATCTTATTTGCAACTGATTCTTTAAAATTTCAAAAATTTTACAACTTAATCCATTCATGTGATGTATTTTTTTATCGCCCCAATCTTTGCTACTAAATGTAACATGTCAATTGTATATATCACTCTGCACATGTATTTTTTGTTTTGTTTGTGTGTATAAATTAAGTGATAATTCTCAAAACATATAGGAATTTTGATTAGTTAATGTAGCTATTCTTACCAGATTCTTCAAATCACGTCCTTTTTATTTTTCTTTAAATTTTTTGCTATGATAAATTCATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATGAGTTAGGATCCGTTGACACCAAAGCTTATTCGTCAGTTCTTTTAATCCAAAGGCCCATAATTAATCCATGTTTCTTTTTCTTGTGTATCATTTCTCTCACCCACACACAATTATTGCTTTTAGTGATAAAAAAAACTCAAACTGAAAACATGGAGTTATGATACTCAACGATTCTCTTTTGGGAAAATTGTTTTTCTCTTCCCCCACTCCTATGAATATTATTCTTCTTCTTTGTATCTCCTTTCCCTCTTACTTAACTCCTCGTATGTATTGCAACACATGAAGGAAATTTATGTCAAGCTTATGATAACAATTACAATTGGAGAGGAATATGGTGAAAGTAGGAGATTGTTGATGAGTTTAATTTGCTTTTAATTTTATGTGTTTTACCATGATTGAATATGAATTAAAATAATCCTAGGATTTTATCGTTTTAAACGATTAGATGTCTACAAAATGAATCGTATGGTTTTGGTAGAATGGAAAACCTCATTACAATTTAATTTTATTATTTACAGCATAAACAATGAAATCTCAACAAAGTCACCACAAGTTTGGGAAGGGTTGTGAATTGGTTCAATTAGCATGTAGGAGAAGAGATCATTCTAAACAGTACTATCTTAAAAGTATTTTAATTATTCAGATATGGAGTATTTCAATTTTGTAGAAAAGAGAACAAAGAGAATTATGATAGGATTATGTTTCCAATTTGGGTTTGGTAATCATTAAAAACAATAATAGTGTGTGATTGAAAAAAAGGATACGTAATAAATAAAAATGTGACACTATTATTAACCTTTGATTAAATAAAATCAACGGTTGAGATTTGGTGTCAATGAATCCGTTGACACCTGGTGTCAACGGATCCTGACTCTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTATTTTTGTTGTTCCTGTCGTCACTTTTTGACAATGCAACTTTTCATTTCATATTATGGTAAAGACCAGAATGTATTGTTGAAGTATTGAATTGCTTTGCTTGTTTTTGGTGTTTATCAGGCGAGTTATGCTGCATTTCTTGACGATGTTGTCTTGCACATGGAAGCTATGACCCCAGAGCAACTAATGCTTTCAATTATGGATGACGATTTTCTTGAAATAGCCGATCTTGTGTAA
Protein sequence

>MS.gene09424.t1

MELLPPGVGFIPSDELLVIYYLANKNNEEVQNFNGSDLIKELNLYQYDPFELRLTPSFFFRHPREFYCFTAKVDEQMRQCKIGFWMQKGAVRDIIVGTDNVLVGKKSLFMFYIGNSAQNGIKTSWTMYEYTLANNPQAPFVLCRVFDCPPEMDLDVEFGADVDEGDATGDGSANKLNEVTQKPMGARDGAGTSAAVEQRGASNFTDFEGTLQASYAAFLDDVVLHMEAMTPEQLMLSIMDDDFLEIADLV