AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene09591

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09591.t1 MTR_5g015680 97.554 368 9 0 3 370 47 414 0.0 754
MS.gene09591.t1 MTR_5g015680 97.554 368 9 0 3 370 8 375 0.0 753
MS.gene09591.t1 MTR_5g015680 98.214 280 5 0 91 370 1 280 0.0 572
MS.gene09591.t1 MTR_4g104140 44.781 297 159 5 63 355 17 312 7.33e-83 256
MS.gene09591.t1 MTR_4g104140 46.218 238 123 5 121 355 7 242 5.64e-67 213
MS.gene09591.t1 MTR_3g075270 36.923 260 163 1 103 362 7 265 4.08e-64 207
MS.gene09591.t1 MTR_4g005050 34.426 122 66 4 243 364 1598 1705 4.73e-15 77.4
MS.gene09591.t1 MTR_3g088660 29.412 153 101 3 76 226 97 244 7.21e-13 68.2
MS.gene09591.t1 MTR_5g085310 38.356 73 44 1 273 345 953 1024 1.70e-12 69.3
MS.gene09591.t1 MTR_3g107960 39.506 81 47 2 274 353 1119 1198 5.76e-12 67.8
MS.gene09591.t1 MTR_6g017010 38.889 72 43 1 274 345 685 755 9.01e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene09591.t1 AT5G67480 65.000 360 114 2 11 369 20 368 9.31e-178 498
MS.gene09591.t1 AT5G67480 65.000 360 114 2 11 369 20 368 9.31e-178 498
MS.gene09591.t1 AT5G67480 65.000 360 114 2 11 369 31 379 1.52e-177 498
MS.gene09591.t1 AT4G37610 60.976 328 126 2 41 367 34 360 8.44e-150 427
MS.gene09591.t1 AT1G05690 49.861 361 167 4 4 360 1 351 1.56e-110 327
MS.gene09591.t1 AT3G48360 43.789 322 163 8 56 361 28 347 2.20e-79 248
MS.gene09591.t1 AT5G63160 43.770 313 163 7 61 361 24 335 8.65e-76 239
MS.gene09591.t1 AT3G48360 45.862 290 142 6 56 331 28 316 1.55e-73 232
MS.gene09591.t1 AT5G63160 46.099 282 140 6 61 331 24 304 2.64e-71 225
MS.gene09591.t1 AT5G63160 45.020 251 127 7 120 361 2 250 8.38e-60 194
MS.gene09591.t1 AT3G48360 44.033 243 122 7 131 361 1 241 1.30e-58 191
MS.gene09591.t1 AT1G79000 29.577 142 82 4 223 364 1546 1669 6.41e-15 77.0
MS.gene09591.t1 AT1G79000 29.577 142 82 4 223 364 1570 1693 6.45e-15 77.0
MS.gene09591.t1 AT1G79000 29.577 142 82 4 223 364 1570 1693 6.48e-15 77.0
MS.gene09591.t1 AT1G16710 28.873 142 83 4 223 364 1550 1673 9.78e-13 70.5
MS.gene09591.t1 AT1G16710 28.873 142 83 4 223 364 1553 1676 9.96e-13 70.5
MS.gene09591.t1 AT1G16710 28.873 142 83 4 223 364 1553 1676 9.96e-13 70.5
MS.gene09591.t1 AT1G16710 28.873 142 83 4 223 364 1553 1676 9.96e-13 70.5
MS.gene09591.t1 AT1G16710 28.873 142 83 4 223 364 1553 1676 9.96e-13 70.5
MS.gene09591.t1 AT1G16710 28.873 142 83 4 223 364 1514 1637 1.03e-12 70.1
MS.gene09591.t1 AT1G16710 28.873 142 83 4 223 364 1514 1637 1.03e-12 70.1
MS.gene09591.t1 AT1G16710 28.873 142 83 4 223 364 1514 1637 1.03e-12 70.1
MS.gene09591.t1 AT1G16710 28.873 142 83 4 223 364 1579 1702 1.07e-12 70.1
MS.gene09591.t1 AT3G12980 36.471 85 50 2 276 360 1581 1661 4.79e-11 65.1
MS.gene09591.t1 AT3G12980 36.471 85 50 2 276 360 1500 1580 4.80e-11 65.1

Find 74 sgRNAs with CRISPR-Local

Find 133 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGAAATTAGATGGACAATTT+TGG 0.186306 5.2:+7458204 MS.gene09591:CDS
GGCGATCGATCTCAATCTTT+GGG 0.258443 5.2:+7456834 MS.gene09591:CDS
TGGCGATCGATCTCAATCTT+TGG 0.276993 5.2:+7456833 MS.gene09591:CDS
TTCGATATTTGTACTCTTCC+TGG 0.283350 5.2:+7456885 MS.gene09591:CDS
ATCTTGAAAACAAGAGGAAT+TGG 0.329795 5.2:+7458235 MS.gene09591:CDS
AAGCAATTCAAGCCCTTTGC+AGG 0.347578 5.2:-7457924 None:intergenic
TTGGGATTGCACCATGCTTT+CGG 0.361459 5.2:+7458254 MS.gene09591:CDS
AAAGAACTTCAAAACTGTTT+CGG 0.374386 5.2:+7457546 MS.gene09591:CDS
AGTTCTAGAAAAGGAAATTC+TGG 0.390373 5.2:+7457606 MS.gene09591:CDS
CGCATACTCGCAAGGATAAC+CGG 0.402463 5.2:-7456222 None:intergenic
GCAAGAAAGACGAAATTAGA+TGG 0.406485 5.2:+7458194 MS.gene09591:CDS
AAAGGAACTCTACAGAAATC+TGG 0.408140 5.2:-7458049 None:intergenic
ATGTGAACAGAAGCTAGAAT+TGG 0.410891 5.2:+7457429 MS.gene09591:CDS
AGAGATGGTTGTCGAACTAT+TGG 0.415706 5.2:+7457854 MS.gene09591:CDS
GGTTGCAAGTTGAGAGTCCC+TGG 0.418562 5.2:+7457962 MS.gene09591:CDS
CTAGTTCACATTTGTAGAGA+TGG 0.440223 5.2:+7457839 MS.gene09591:CDS
CGAGGCGAAGGAGGTGGTAT+CGG 0.446905 5.2:-7456188 None:intergenic
TCTGTAGAGTTCCTTTGTGC+AGG 0.450851 5.2:+7458056 MS.gene09591:CDS
TCTTGAAAACAAGAGGAATT+GGG 0.451569 5.2:+7458236 MS.gene09591:CDS
TTCAGCATGCCTCTCAATAC+AGG 0.459183 5.2:-7456788 None:intergenic
TGAGATCGATCGCCACCGAT+TGG 0.468351 5.2:-7456825 None:intergenic
GGAATTTGTCCTACATTTGT+TGG 0.476702 5.2:+7457366 MS.gene09591:CDS
GAGTCACCCAGTTCTAGAAA+AGG 0.485308 5.2:+7457597 MS.gene09591:CDS
GTTTGCATTAATACTGATAA+CGG 0.495992 5.2:+7456344 MS.gene09591:CDS
TGTGAACAGAAGCTAGAATT+GGG 0.501782 5.2:+7457430 MS.gene09591:CDS
ACACAGCTGCTTCGCCCTCT+CGG 0.509722 5.2:-7456263 None:intergenic
TTACGAGAAAGAAGAAATGA+AGG 0.513436 5.2:+7457345 MS.gene09591:CDS
TTCTGCAACAAGAGATCTGT+GGG 0.515476 5.2:+7456289 MS.gene09591:CDS
TCGCCTCGATCAGCAGCAAC+CGG 0.515743 5.2:+7456203 MS.gene09591:CDS
GTTGCAAGTTGAGAGTCCCT+GGG 0.520761 5.2:+7457963 MS.gene09591:CDS
CACATTCACGCTTCAAGTGA+GGG 0.529306 5.2:-7457411 None:intergenic
GTGCTCATTGCAAGAGGATG+TGG 0.529329 5.2:+7457990 MS.gene09591:CDS
ATAAGCTGATGGCTAACATT+CGG 0.529455 5.2:+7456147 None:intergenic
CAAGAGGATGTGGCAACTGT+TGG 0.537744 5.2:+7458000 MS.gene09591:CDS
ATTGATTGATCAGAGTAAGA+AGG 0.539090 5.2:+7457754 MS.gene09591:intron
CTTTGAAGTCTTTATCATGC+GGG 0.542449 5.2:-7457877 None:intergenic
AAAACTGTTTCGGAGTCAGA+AGG 0.543714 5.2:+7457556 MS.gene09591:CDS
GAGAAAGCACCAACAAATGT+AGG 0.546221 5.2:-7457375 None:intergenic
AGCAAGCAAATCGTTCCAAT+CGG 0.549306 5.2:+7456810 MS.gene09591:CDS
GTTGCTGCTGATCGAGGCGA+AGG 0.550246 5.2:-7456200 None:intergenic
TTTGCAATTATACGATGCAA+TGG 0.552736 5.2:+7457811 MS.gene09591:CDS
GTAGGTATACTTCCTGCAAA+GGG 0.554458 5.2:+7457912 MS.gene09591:CDS
GCTGATCGAGGCGAAGGAGG+TGG 0.554736 5.2:-7456194 None:intergenic
ATCAGTATTAATGCAAACAT+CGG 0.559796 5.2:-7456339 None:intergenic
AGGAAGTATACCTACACGGC+TGG 0.561333 5.2:-7457904 None:intergenic
GAAAAGATCTTGAAAACAAG+AGG 0.566400 5.2:+7458229 MS.gene09591:CDS
GCTTTGAAGTCTTTATCATG+CGG 0.567781 5.2:-7457878 None:intergenic
TTCATCGCCGCATACTCGCA+AGG 0.575928 5.2:-7456230 None:intergenic
ATGAATAATTTATCACCGAG+AGG 0.575964 5.2:+7456248 MS.gene09591:CDS
GCTGCTGATCGAGGCGAAGG+AGG 0.588376 5.2:-7456197 None:intergenic
TGTAGGTATACTTCCTGCAA+AGG 0.593266 5.2:+7457911 MS.gene09591:CDS
TTGATCAGAGTAAGAAGGAG+AGG 0.595421 5.2:+7457759 MS.gene09591:intron
TCACATTCACGCTTCAAGTG+AGG 0.596344 5.2:-7457412 None:intergenic
CGGTTATCCTTGCGAGTATG+CGG 0.602040 5.2:+7456223 MS.gene09591:CDS
CTGTTTCGGAGTCAGAAGGA+TGG 0.605228 5.2:+7457560 MS.gene09591:CDS
AAGCAAATCGTTCCAATCGG+TGG 0.607764 5.2:+7456813 MS.gene09591:CDS
TCAAAGCAAACCAGCCGTGT+AGG 0.611970 5.2:+7457894 MS.gene09591:CDS
TGTCATTGTGGCCGAAAGCA+TGG 0.612906 5.2:-7458265 None:intergenic
TAACCGGTTGCTGCTGATCG+AGG 0.614451 5.2:-7456206 None:intergenic
GGGGTTGTGCTCATTGCAAG+AGG 0.622565 5.2:+7457984 MS.gene09591:CDS
AAAACTCGTACTGCATCATG+TGG 0.623793 5.2:-7456860 None:intergenic
GTGGCTTCTCCTGTATTGAG+AGG 0.626268 5.2:+7456779 MS.gene09591:CDS
TACATCAACTAGATTATCCA+CGG 0.627170 5.2:-7457461 None:intergenic
GCGATCGATCTCAATCTTTG+GGG 0.627705 5.2:+7456835 MS.gene09591:CDS
CTTCTGCAACAAGAGATCTG+TGG 0.636785 5.2:+7456288 MS.gene09591:CDS
TGAATAATTTATCACCGAGA+GGG 0.647099 5.2:+7456249 MS.gene09591:CDS
TGCATTAATACTGATAACGG+TGG 0.661014 5.2:+7456347 MS.gene09591:CDS
TTGCAAGTTGAGAGTCCCTG+GGG 0.661286 5.2:+7457964 MS.gene09591:CDS
TAATATTGTTACCTGCACAA+AGG 0.667930 5.2:-7458067 None:intergenic
AGAATTGGGTTTACTCACCG+TGG 0.691467 5.2:+7457444 MS.gene09591:CDS
TTGCAGGAAGTATACCTACA+CGG 0.699708 5.2:-7457908 None:intergenic
TGCAAGTTGAGAGTCCCTGG+GGG 0.717689 5.2:+7457965 MS.gene09591:CDS
CATTACTGTGTGATGCTCCG+CGG 0.722450 5.2:+7457494 MS.gene09591:CDS
TGACAAATGAGACTGAGCCG+CGG 0.747022 5.2:-7457511 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTACACAATAATAGATAATT+AGG + chr5.2:7457706-7457725 MS.gene09591:intron 15.0%
!!! AAAAAAAATTGAGTTTTGTT+TGG + chr5.2:7457170-7457189 MS.gene09591:intron 15.0%
!!! AAAAAAATTGAGTTTTGTTT+GGG + chr5.2:7457171-7457190 MS.gene09591:intron 15.0%
!!! AAACTTTAAGAAATTTCAAT+CGG + chr5.2:7457065-7457084 MS.gene09591:intron 15.0%
!!! TATGCAATTAATCTATTTTA+AGG + chr5.2:7456560-7456579 MS.gene09591:intron 15.0%
!!! AAAAAATTGAGTTTTGTTTG+GGG + chr5.2:7457172-7457191 MS.gene09591:intron 20.0%
!!! ATGATTTTGAGGTTTATAAA+GGG - chr5.2:7456921-7456940 None:intergenic 20.0%
!!! ATTTCTTAAAGTTTTCCTAT+CGG - chr5.2:7457059-7457078 None:intergenic 20.0%
!!! TATGATTTTGAGGTTTATAA+AGG - chr5.2:7456922-7456941 None:intergenic 20.0%
!!! TGAAGTTCTTTAGTATTTTA+CGG - chr5.2:7457537-7457556 None:intergenic 20.0%
! AAAGAACTTCAAAACTGTTT+CGG + chr5.2:7457546-7457565 MS.gene09591:CDS 25.0%
! AACTGTGAAATTGAAACAAA+TGG + chr5.2:7457141-7457160 MS.gene09591:intron 25.0%
! ATCAGTATTAATGCAAACAT+CGG - chr5.2:7456342-7456361 None:intergenic 25.0%
! ATTCAAAAGTAGTTAGACAA+TGG - chr5.2:7457732-7457751 None:intergenic 25.0%
! GTTTGCATTAATACTGATAA+CGG + chr5.2:7456344-7456363 MS.gene09591:CDS 25.0%
! TCACTTTATACTATATGATC+AGG + chr5.2:7456754-7456773 MS.gene09591:intron 25.0%
! TGATTGAAGAAGAAAATGTA+AGG + chr5.2:7457635-7457654 MS.gene09591:intron 25.0%
! TGATTGTATTTATTTCAGCT+CGG + chr5.2:7458105-7458124 MS.gene09591:intron 25.0%
!! CAAACATTCTTATGATTTTG+AGG - chr5.2:7456932-7456951 None:intergenic 25.0%
!! GTAACAAATCTTTTCATTTC+AGG + chr5.2:7458143-7458162 MS.gene09591:intron 25.0%
!!! AAAAATTGAGTTTTGTTTGG+GGG + chr5.2:7457173-7457192 MS.gene09591:intron 25.0%
ATCTTGAAAACAAGAGGAAT+TGG + chr5.2:7458235-7458254 MS.gene09591:CDS 30.0%
ATGAATAATTTATCACCGAG+AGG + chr5.2:7456248-7456267 MS.gene09591:CDS 30.0%
ATTGATTGATCAGAGTAAGA+AGG + chr5.2:7457754-7457773 MS.gene09591:intron 30.0%
CAGAAAAATGAATGAAAGAG+AGG + chr5.2:7457784-7457803 MS.gene09591:CDS 30.0%
CTTTATACTATATGATCAGG+CGG + chr5.2:7456757-7456776 MS.gene09591:intron 30.0%
GAAAAGATCTTGAAAACAAG+AGG + chr5.2:7458229-7458248 MS.gene09591:CDS 30.0%
TAAGAAATTTCAATCGGTAG+TGG + chr5.2:7457071-7457090 MS.gene09591:intron 30.0%
TAATATTGTTACCTGCACAA+AGG - chr5.2:7458070-7458089 None:intergenic 30.0%
TACATCAACTAGATTATCCA+CGG - chr5.2:7457464-7457483 None:intergenic 30.0%
TACTTCGAAATTGATCTACT+CGG - chr5.2:7457117-7457136 None:intergenic 30.0%
TCTTGAAAACAAGAGGAATT+GGG + chr5.2:7458236-7458255 MS.gene09591:CDS 30.0%
TCTTTCTCGTAACTGAAAAA+AGG - chr5.2:7457337-7457356 None:intergenic 30.0%
TCTTTGAACGAATTAGTAGT+AGG + chr5.2:7456505-7456524 MS.gene09591:intron 30.0%
TGAATAATTTATCACCGAGA+GGG + chr5.2:7456249-7456268 MS.gene09591:CDS 30.0%
TTACGAGAAAGAAGAAATGA+AGG + chr5.2:7457345-7457364 MS.gene09591:CDS 30.0%
TTTGCAATTATACGATGCAA+TGG + chr5.2:7457811-7457830 MS.gene09591:CDS 30.0%
! AGTAGGCTCAAAATTTTCAT+AGG + chr5.2:7456522-7456541 MS.gene09591:intron 30.0%
! CGAAATTAGATGGACAATTT+TGG + chr5.2:7458204-7458223 MS.gene09591:CDS 30.0%
!! AGTTCTAGAAAAGGAAATTC+TGG + chr5.2:7457606-7457625 MS.gene09591:CDS 30.0%
!!! AAAATTGAGTTTTGTTTGGG+GGG + chr5.2:7457174-7457193 MS.gene09591:intron 30.0%
!!! AGAATTTCCTTTTCTAGAAC+TGG - chr5.2:7457607-7457626 None:intergenic 30.0%
!!! GAATTTCCTTTTCTAGAACT+GGG - chr5.2:7457606-7457625 None:intergenic 30.0%
AAAGGAACTCTACAGAAATC+TGG - chr5.2:7458052-7458071 None:intergenic 35.0%
ATAAAGGGAACAAACATACC+AGG - chr5.2:7456906-7456925 None:intergenic 35.0%
ATGTGAACAGAAGCTAGAAT+TGG + chr5.2:7457429-7457448 MS.gene09591:CDS 35.0%
CTAGTTCACATTTGTAGAGA+TGG + chr5.2:7457839-7457858 MS.gene09591:CDS 35.0%
GCAAGAAAGACGAAATTAGA+TGG + chr5.2:7458194-7458213 MS.gene09591:CDS 35.0%
GGAATTTGTCCTACATTTGT+TGG + chr5.2:7457366-7457385 MS.gene09591:CDS 35.0%
GTGCCTATACAAAATCAAGT+GGG - chr5.2:7456415-7456434 None:intergenic 35.0%
TGTGAACAGAAGCTAGAATT+GGG + chr5.2:7457430-7457449 MS.gene09591:CDS 35.0%
TTCGATATTTGTACTCTTCC+TGG + chr5.2:7456885-7456904 MS.gene09591:CDS 35.0%
! CACTTGATTTTGTATAGGCA+CGG + chr5.2:7456414-7456433 MS.gene09591:intron 35.0%
! CATCCCACTTGATTTTGTAT+AGG + chr5.2:7456409-7456428 MS.gene09591:intron 35.0%
!! CTAGGGTTGATAATATTCTC+TGG + chr5.2:7456970-7456989 MS.gene09591:intron 35.0%
!! CTTTGAAGTCTTTATCATGC+GGG - chr5.2:7457880-7457899 None:intergenic 35.0%
!! GAAATTTTGATGTACCTAGC+CGG - chr5.2:7456446-7456465 None:intergenic 35.0%
!! GCTTTGAAGTCTTTATCATG+CGG - chr5.2:7457881-7457900 None:intergenic 35.0%
!! TGCATTAATACTGATAACGG+TGG + chr5.2:7456347-7456366 MS.gene09591:CDS 35.0%
AAAACTCGTACTGCATCATG+TGG - chr5.2:7456863-7456882 None:intergenic 40.0%
AAAACTGTTTCGGAGTCAGA+AGG + chr5.2:7457556-7457575 MS.gene09591:CDS 40.0%
AGAGATGGTTGTCGAACTAT+TGG + chr5.2:7457854-7457873 MS.gene09591:CDS 40.0%
AGCAAGCAAATCGTTCCAAT+CGG + chr5.2:7456810-7456829 MS.gene09591:CDS 40.0%
ATTATCAACCCTAGCCCTAT+AGG - chr5.2:7456964-7456983 None:intergenic 40.0%
ATTGATCTACTCGGTTTAGC+TGG - chr5.2:7457108-7457127 None:intergenic 40.0%
CGTGCCTATACAAAATCAAG+TGG - chr5.2:7456416-7456435 None:intergenic 40.0%
GAGATCAACATAGCTGATCA+AGG + chr5.2:7457205-7457224 MS.gene09591:intron 40.0%
GTAGGTATACTTCCTGCAAA+GGG + chr5.2:7457912-7457931 MS.gene09591:CDS 40.0%
GTTTGTCTCCTTGTCCTATA+GGG + chr5.2:7456947-7456966 MS.gene09591:intron 40.0%
TACATCCATTCATTGCCGAT+AGG + chr5.2:7457041-7457060 MS.gene09591:intron 40.0%
TATACTATATGATCAGGCGG+TGG + chr5.2:7456760-7456779 MS.gene09591:intron 40.0%
TGTAGGTATACTTCCTGCAA+AGG + chr5.2:7457911-7457930 MS.gene09591:CDS 40.0%
TGTTTGTCTCCTTGTCCTAT+AGG + chr5.2:7456946-7456965 MS.gene09591:intron 40.0%
TTCTGCAACAAGAGATCTGT+GGG + chr5.2:7456289-7456308 MS.gene09591:CDS 40.0%
TTGATCAGAGTAAGAAGGAG+AGG + chr5.2:7457759-7457778 MS.gene09591:intron 40.0%
! GTTTTCCTATCGGCAATGAA+TGG - chr5.2:7457049-7457068 None:intergenic 40.0%
! TTGCAGGAAGTATACCTACA+CGG - chr5.2:7457911-7457930 None:intergenic 40.0%
!! GAGAAAGCACCAACAAATGT+AGG - chr5.2:7457378-7457397 None:intergenic 40.0%
!! TTGCTTGTTCGTCATTTTGC+TGG + chr5.2:7457941-7457960 MS.gene09591:CDS 40.0%
AAGCAAATCGTTCCAATCGG+TGG + chr5.2:7456813-7456832 MS.gene09591:CDS 45.0%
AAGCAATTCAAGCCCTTTGC+AGG - chr5.2:7457927-7457946 None:intergenic 45.0%
AGAATTGGGTTTACTCACCG+TGG + chr5.2:7457444-7457463 MS.gene09591:CDS 45.0%
CACATTCACGCTTCAAGTGA+GGG - chr5.2:7457414-7457433 None:intergenic 45.0%
CTCTTAAGCAGTGACATCTG+TGG - chr5.2:7456586-7456605 None:intergenic 45.0%
CTTCTGCAACAAGAGATCTG+TGG + chr5.2:7456288-7456307 MS.gene09591:CDS 45.0%
GCGATCGATCTCAATCTTTG+GGG + chr5.2:7456835-7456854 MS.gene09591:CDS 45.0%
GGCGATCGATCTCAATCTTT+GGG + chr5.2:7456834-7456853 MS.gene09591:CDS 45.0%
TCACATTCACGCTTCAAGTG+AGG - chr5.2:7457415-7457434 None:intergenic 45.0%
TCTGTAGAGTTCCTTTGTGC+AGG + chr5.2:7458056-7458075 MS.gene09591:CDS 45.0%
TGGCGATCGATCTCAATCTT+TGG + chr5.2:7456833-7456852 MS.gene09591:CDS 45.0%
TTCAGCATGCCTCTCAATAC+AGG - chr5.2:7456791-7456810 None:intergenic 45.0%
TTGGGATTGCACCATGCTTT+CGG + chr5.2:7458254-7458273 MS.gene09591:CDS 45.0%
!! GAGTCACCCAGTTCTAGAAA+AGG + chr5.2:7457597-7457616 MS.gene09591:CDS 45.0%
!!! ATTGAGTTTTGTTTGGGGGG+TGG + chr5.2:7457177-7457196 MS.gene09591:intron 45.0%
!!! GGGAGCGTCTTTTCAATGAA+GGG + chr5.2:7456309-7456328 MS.gene09591:CDS 45.0%
!!! TGGGAGCGTCTTTTCAATGA+AGG + chr5.2:7456308-7456327 MS.gene09591:CDS 45.0%
!!! TTGAGTTTTGTTTGGGGGGT+GGG + chr5.2:7457178-7457197 MS.gene09591:intron 45.0%
!! AAACAAAAAATTAATAATTA+AGG - chr5.2:7457674-7457693 None:intergenic 5.0%
AACCCTAGCCCTATAGGACA+AGG - chr5.2:7456958-7456977 None:intergenic 50.0%
CAAGAGGATGTGGCAACTGT+TGG + chr5.2:7458000-7458019 MS.gene09591:CDS 50.0%
CATTACTGTGTGATGCTCCG+CGG + chr5.2:7457494-7457513 MS.gene09591:CDS 50.0%
CGCATACTCGCAAGGATAAC+CGG - chr5.2:7456225-7456244 None:intergenic 50.0%
CGGTTATCCTTGCGAGTATG+CGG + chr5.2:7456223-7456242 MS.gene09591:CDS 50.0%
CTCCTTGTCCTATAGGGCTA+GGG + chr5.2:7456953-7456972 MS.gene09591:intron 50.0%
CTGTTTCGGAGTCAGAAGGA+TGG + chr5.2:7457560-7457579 MS.gene09591:CDS 50.0%
GTGCTCATTGCAAGAGGATG+TGG + chr5.2:7457990-7458009 MS.gene09591:CDS 50.0%
GTGGCTTCTCCTGTATTGAG+AGG + chr5.2:7456779-7456798 MS.gene09591:CDS 50.0%
TCAAAGCAAACCAGCCGTGT+AGG + chr5.2:7457894-7457913 MS.gene09591:CDS 50.0%
TCTACTCGGTTTAGCTGGTG+AGG - chr5.2:7457103-7457122 None:intergenic 50.0%
TCTCCTTGTCCTATAGGGCT+AGG + chr5.2:7456952-7456971 MS.gene09591:intron 50.0%
TGACAAATGAGACTGAGCCG+CGG - chr5.2:7457514-7457533 None:intergenic 50.0%
! AGGAAGTATACCTACACGGC+TGG - chr5.2:7457907-7457926 None:intergenic 50.0%
! GTTGCAAGTTGAGAGTCCCT+GGG + chr5.2:7457963-7457982 MS.gene09591:CDS 50.0%
! TTGCAAGTTGAGAGTCCCTG+GGG + chr5.2:7457964-7457983 MS.gene09591:CDS 50.0%
!!! TGAGTTTTGTTTGGGGGGTG+GGG + chr5.2:7457179-7457198 MS.gene09591:intron 50.0%
GGGGTTGTGCTCATTGCAAG+AGG + chr5.2:7457984-7458003 MS.gene09591:CDS 55.0%
TAACCGGTTGCTGCTGATCG+AGG - chr5.2:7456209-7456228 None:intergenic 55.0%
TGAGATCGATCGCCACCGAT+TGG - chr5.2:7456828-7456847 None:intergenic 55.0%
TGCAATGAGCACAACCCCCA+GGG - chr5.2:7457982-7458001 None:intergenic 55.0%
TGTATAGGCACGGATGCTGC+CGG + chr5.2:7456424-7456443 MS.gene09591:intron 55.0%
TTCATCGCCGCATACTCGCA+AGG - chr5.2:7456233-7456252 None:intergenic 55.0%
TTGCAATGAGCACAACCCCC+AGG - chr5.2:7457983-7458002 None:intergenic 55.0%
! GGTTGCAAGTTGAGAGTCCC+TGG + chr5.2:7457962-7457981 MS.gene09591:CDS 55.0%
! TGCAAGTTGAGAGTCCCTGG+GGG + chr5.2:7457965-7457984 MS.gene09591:CDS 55.0%
!!! GAGTTTTGTTTGGGGGGTGG+GGG + chr5.2:7457180-7457199 MS.gene09591:intron 55.0%
ACACAGCTGCTTCGCCCTCT+CGG - chr5.2:7456266-7456285 None:intergenic 60.0%
CGAGGCGAAGGAGGTGGTAT+CGG - chr5.2:7456191-7456210 None:intergenic 60.0%
GTTGCTGCTGATCGAGGCGA+AGG - chr5.2:7456203-7456222 None:intergenic 60.0%
TCGCCTCGATCAGCAGCAAC+CGG + chr5.2:7456203-7456222 MS.gene09591:CDS 60.0%
!! TTTTGTTTGGGGGGTGGGGG+AGG + chr5.2:7457183-7457202 MS.gene09591:intron 60.0%
GCTGATCGAGGCGAAGGAGG+TGG - chr5.2:7456197-7456216 None:intergenic 65.0%
GCTGCTGATCGAGGCGAAGG+AGG - chr5.2:7456200-7456219 None:intergenic 65.0%
! AGGCACGGATGCTGCCGGCT+AGG + chr5.2:7456429-7456448 MS.gene09591:intron 70.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 7456155 7458285 7456155 ID=MS.gene09591
chr5.2 mRNA 7456155 7458285 7456155 ID=MS.gene09591.t1;Parent=MS.gene09591
chr5.2 exon 7456155 7456397 7456155 ID=MS.gene09591.t1.exon1;Parent=MS.gene09591.t1
chr5.2 CDS 7456155 7456397 7456155 ID=cds.MS.gene09591.t1;Parent=MS.gene09591.t1
chr5.2 exon 7456776 7456906 7456776 ID=MS.gene09591.t1.exon2;Parent=MS.gene09591.t1
chr5.2 CDS 7456776 7456906 7456776 ID=cds.MS.gene09591.t1;Parent=MS.gene09591.t1
chr5.2 exon 7457345 7457651 7457345 ID=MS.gene09591.t1.exon3;Parent=MS.gene09591.t1
chr5.2 CDS 7457345 7457651 7457345 ID=cds.MS.gene09591.t1;Parent=MS.gene09591.t1
chr5.2 exon 7457767 7458077 7457767 ID=MS.gene09591.t1.exon4;Parent=MS.gene09591.t1
chr5.2 CDS 7457767 7458077 7457767 ID=cds.MS.gene09591.t1;Parent=MS.gene09591.t1
chr5.2 exon 7458165 7458285 7458165 ID=MS.gene09591.t1.exon5;Parent=MS.gene09591.t1
chr5.2 CDS 7458165 7458285 7458165 ID=cds.MS.gene09591.t1;Parent=MS.gene09591.t1
Gene Sequence

>MS.gene09591

ATGGCTAACATTCGGAATGCTAAGAAATTCATTCCGATACCACCTCCTTCGCCTCGATCAGCAGCAACCGGTTATCCTTGCGAGTATGCGGCGATGAATAATTTATCACCGAGAGGGCGAAGCAGCTGTGTCTCTTCTGCAACAAGAGATCTGTGGGAGCGTCTTTTCAATGAAGGGTATAAAGCCGATGTTTGCATTAATACTGATAACGGTGGCATTGTTTATGCTCATTCTAACATTATTGTAAGTTCATTCATCCCACTTGATTTTGTATAGGCACGGATGCTGCCGGCTAGGTACATCAAAATTTCATTGTCAAAATGTTTTTTTCAATAAACTGTGCTCGTATTTCTTTGAACGAATTAGTAGTAGGCTCAAAATTTTCATAGGCTCAGCAGATATAGTTATGCAATTAATCTATTTTAAGGCCACAGATGTCACTGCTTAAGAGTTGATTAATCAAGAGATAACTAGACATGCTTAGTATGATACTGTTGTTGTATGATCTGCTCTCATCATGAAAAACTGATATTTTGTCATTAAACATTAATGTGAATGATCTTTATGTTAATCTTGAATTCAACTGTTGTGAATATCACTCACTTTATACTATATGATCAGGCGGTGGCTTCTCCTGTATTGAGAGGCATGCTGAAGCAAGCAAATCGTTCCAATCGGTGGCGATCGATCTCAATCTTTGGGGTTCCACATGATGCAGTACGAGTTTTTATTCGATATTTGTACTCTTCCTGGTATGTTTGTTCCCTTTATAAACCTCAAAATCATAAGAATGTTTGTCTCCTTGTCCTATAGGGCTAGGGTTGATAATATTCTCTGGCATTATATTTGCTTAAATTAGATGTCACTGCTTGATTTTATATGCAAATACATCCATTCATTGCCGATAGGAAAACTTTAAGAAATTTCAATCGGTAGTGGAGAGTTCCTCACCAGCTAAACCGAGTAGATCAATTTCGAAGTAGTAGAACTGTGAAATTGAAACAAATGGAAAGAAAAAAAAAATTGAGTTTTGTTTGGGGGGTGGGGGAGGAGATCAACATAGCTGATCAAGGTTTTAGAGTTACTACTATACTTTTCTGAATGCGCTGTAGTTGAATGCATTGTAGTTGCTGTGTCTGTAATGCATTTTGCATCATATAAATGTGCTGATTTTTTTTGCCTTTTTTCAGTTACGAGAAAGAAGAAATGAAGGAATTTGTCCTACATTTGTTGGTGCTTTCTCATGTATACGCAGTCCCTCACTTGAAGCGTGAATGTGAACAGAAGCTAGAATTGGGTTTACTCACCGTGGATAATCTAGTTGATGTATTTCAACTTGCATTACTGTGTGATGCTCCGCGGCTCAGTCTCATTTGTCACCGTAAAATACTAAAGAACTTCAAAACTGTTTCGGAGTCAGAAGGATGGAAAGCAATGAAGCAGAGTCACCCAGTTCTAGAAAAGGAAATTCTGGAATCAATGATTGAAGAAGAAAATGTAAGGCTCTGTCTTTGCACCTTAATTATTAATTTTTTGTTTTTGTTTTGTATTTTACACAATAATAGATAATTAGGCCATTGTCTAACTACTTTTGAATGAATTGATTGATCAGAGTAAGAAGGAGAGGATCAGAAAAATGAATGAAAGAGAGGTCTATTTGCAATTATACGATGCAATGGAAGCTCTAGTTCACATTTGTAGAGATGGTTGTCGAACTATTGGCCCGCATGATAAAGACTTCAAAGCAAACCAGCCGTGTAGGTATACTTCCTGCAAAGGGCTTGAATTGCTTGTTCGTCATTTTGCTGGTTGCAAGTTGAGAGTCCCTGGGGGTTGTGCTCATTGCAAGAGGATGTGGCAACTGTTGGAGCTGCATTCTCGACTGTGTGCTGATCCAGATTTCTGTAGAGTTCCTTTGTGCAGGTAACAATATTACGTGTTTTCTAACTATGATTGTATTTATTTCAGCTCGGTCATTCAGATAAATTGTAACAAATCTTTTCATTTCAGGAACTTTAAGCAGAGAATAGCAAAACAAAGCAAGAAAGACGAAATTAGATGGACAATTTTGGTTGAAAAGATCTTGAAAACAAGAGGAATTGGGATTGCACCATGCTTTCGGCCACAATGA
Protein sequence

>MS.gene09591.t1

MANIRNAKKFIPIPPPSPRSAATGYPCEYAAMNNLSPRGRSSCVSSATRDLWERLFNEGYKADVCINTDNGGIVYAHSNIIAVASPVLRGMLKQANRSNRWRSISIFGVPHDAVRVFIRYLYSSCYEKEEMKEFVLHLLVLSHVYAVPHLKRECEQKLELGLLTVDNLVDVFQLALLCDAPRLSLICHRKILKNFKTVSESEGWKAMKQSHPVLEKEILESMIEEENSKKERIRKMNEREVYLQLYDAMEALVHICRDGCRTIGPHDKDFKANQPCRYTSCKGLELLVRHFAGCKLRVPGGCAHCKRMWQLLELHSRLCADPDFCRVPLCRNFKQRIAKQSKKDEIRWTILVEKILKTRGIGIAPCFRPQ