Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09842.t1 | GAU37750.1 | 39.3 | 183 | 109 | 2 | 29 | 209 | 27 | 209 | 8.40E-30 | 140.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09842.t1 | A0A2Z6NMK1 | 39.3 | 183 | 109 | 2 | 29 | 209 | 27 | 209 | 6.0e-30 | 140.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene09842.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09842.t1 | MTR_4g123470 | 29.560 | 159 | 112 | 0 | 36 | 194 | 52 | 210 | 2.32e-21 | 88.2 |
| MS.gene09842.t1 | MTR_1g054495 | 23.494 | 166 | 126 | 1 | 44 | 209 | 57 | 221 | 1.88e-12 | 64.3 |
| MS.gene09842.t1 | MTR_0014s0220 | 28.472 | 144 | 87 | 3 | 52 | 195 | 683 | 810 | 1.38e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 44 sgRNAs with CRISPR-Local
Find 53 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAAATGTGATGTACTAATA+AGG | 0.256110 | 7.1:-7354873 | MS.gene09842:CDS |
| AATGATCTTTATGAAGTTCT+TGG | 0.265359 | 7.1:-7355203 | MS.gene09842:CDS |
| CAAATGTAATGAAGGTTTCT+AGG | 0.290265 | 7.1:-7354738 | MS.gene09842:CDS |
| TCATAACCTGCAGCCATATA+AGG | 0.313221 | 7.1:+7355061 | None:intergenic |
| ATGATCTTTATGAAGTTCTT+GGG | 0.324200 | 7.1:-7355202 | MS.gene09842:CDS |
| AATGATCTTAACTCCTTATA+TGG | 0.327626 | 7.1:-7355074 | MS.gene09842:CDS |
| CTGAGGGATGGATCAACTTA+AGG | 0.360735 | 7.1:-7355252 | MS.gene09842:CDS |
| GTCCACGAAATGCAGTGAAT+TGG | 0.385092 | 7.1:+7355167 | None:intergenic |
| TAAGCTTAAGTTTAAGTTTG+TGG | 0.399998 | 7.1:-7354773 | MS.gene09842:CDS |
| TATGGCTGCAGGTTATGAAT+TGG | 0.411365 | 7.1:-7355056 | MS.gene09842:intron |
| GAGTTAAGATCATTTCATAA+TGG | 0.420070 | 7.1:+7355083 | None:intergenic |
| GGTGATTACATTAGAAGCTC+AGG | 0.426576 | 7.1:-7354937 | MS.gene09842:CDS |
| TTCCAATTCACTGCATTTCG+TGG | 0.427022 | 7.1:-7355169 | MS.gene09842:CDS |
| CAGACACTGAACATTGACTT+TGG | 0.433491 | 7.1:-7354958 | MS.gene09842:CDS |
| TTCTCTGCTATTAATATGTA+TGG | 0.435001 | 7.1:-7354913 | MS.gene09842:CDS |
| CAAATGTGATGTACTAATAA+GGG | 0.442101 | 7.1:-7354872 | MS.gene09842:CDS |
| TGAGGGATGGATCAACTTAA+GGG | 0.444993 | 7.1:-7355251 | MS.gene09842:CDS |
| TTAACTCCTTATATGGCTGC+AGG | 0.483063 | 7.1:-7355067 | MS.gene09842:CDS |
| ATTAATATGTATGGACCTAC+AGG | 0.484413 | 7.1:-7354904 | MS.gene09842:CDS |
| TGTAAAGACAATGGATTCCA+TGG | 0.503947 | 7.1:-7354802 | MS.gene09842:CDS |
| CAACAAAGATTGGCAGTGGA+TGG | 0.512212 | 7.1:-7354834 | MS.gene09842:CDS |
| CACTTACAAGCAAGGAATTG+AGG | 0.515012 | 7.1:-7355113 | MS.gene09842:CDS |
| AAGTACATCAAATGTAATGA+AGG | 0.524181 | 7.1:-7354746 | MS.gene09842:CDS |
| ATGTCAACTCATTGCCATCA+TGG | 0.539340 | 7.1:-7355138 | MS.gene09842:CDS |
| ATATCTCATTTACAGCCGTA+TGG | 0.553596 | 7.1:-7355398 | MS.gene09842:intron |
| AAGACAATGGATTCCATGGT+GGG | 0.554852 | 7.1:-7354798 | MS.gene09842:CDS |
| ATCCATCCCTCAGTTAGAAG+TGG | 0.556411 | 7.1:+7355262 | None:intergenic |
| ATAAAATGCTCAACAAAGGA+AGG | 0.558305 | 7.1:+7355365 | None:intergenic |
| TGATCTTTATGAAGTTCTTG+GGG | 0.561506 | 7.1:-7355201 | MS.gene09842:CDS |
| AAGGCAACAAAGATTGGCAG+TGG | 0.562007 | 7.1:-7354838 | MS.gene09842:CDS |
| TGCTTGTAAGTGTGCCATGA+TGG | 0.567390 | 7.1:+7355124 | None:intergenic |
| ATCCACTTCTAACTGAGGGA+TGG | 0.574184 | 7.1:-7355264 | MS.gene09842:CDS |
| GTTGATAAAATGCTCAACAA+AGG | 0.587741 | 7.1:+7355361 | None:intergenic |
| CATTTGTACTCGGTGCCTGT+AGG | 0.588159 | 7.1:+7354889 | None:intergenic |
| TAGTACATCACATTTGTACT+CGG | 0.590498 | 7.1:+7354879 | None:intergenic |
| TGTGATGTACTAATAAGGGA+TGG | 0.597036 | 7.1:-7354868 | MS.gene09842:CDS |
| TACTGCTTATATCATCAATG+AGG | 0.608119 | 7.1:-7355484 | MS.gene09842:intron |
| GAAGGTCTAATCTCTCCATA+CGG | 0.613102 | 7.1:+7355383 | None:intergenic |
| TGAACATCCACTTCTAACTG+AGG | 0.624857 | 7.1:-7355269 | MS.gene09842:CDS |
| ACTTAAGCTTATCCCCACCA+TGG | 0.632346 | 7.1:+7354785 | None:intergenic |
| AAAGACAATGGATTCCATGG+TGG | 0.635851 | 7.1:-7354799 | MS.gene09842:CDS |
| AGACAATGGATTCCATGGTG+GGG | 0.640570 | 7.1:-7354797 | MS.gene09842:CDS |
| TCATGGCACACTTACAAGCA+AGG | 0.641070 | 7.1:-7355121 | MS.gene09842:CDS |
| GAACATCCACTTCTAACTGA+GGG | 0.652020 | 7.1:-7355268 | MS.gene09842:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTCTCAACATAAAATAAT+AGG | + | chr7.1:7354780-7354799 | None:intergenic | 15.0% |
| !!! | AACAAATTTTGTAAAGACAA+TGG | - | chr7.1:7355418-7355437 | MS.gene09842:intron | 20.0% |
| ! | AAGTACATCAAATGTAATGA+AGG | - | chr7.1:7355483-7355502 | MS.gene09842:intron | 25.0% |
| ! | AATGATCTTAACTCCTTATA+TGG | - | chr7.1:7355155-7355174 | MS.gene09842:CDS | 25.0% |
| ! | AATGATCTTTATGAAGTTCT+TGG | - | chr7.1:7355026-7355045 | MS.gene09842:intron | 25.0% |
| ! | ACAAATGTGATGTACTAATA+AGG | - | chr7.1:7355356-7355375 | MS.gene09842:CDS | 25.0% |
| ! | ATGATCTTTATGAAGTTCTT+GGG | - | chr7.1:7355027-7355046 | MS.gene09842:intron | 25.0% |
| ! | CAAATGTGATGTACTAATAA+GGG | - | chr7.1:7355357-7355376 | MS.gene09842:CDS | 25.0% |
| ! | GAGTTAAGATCATTTCATAA+TGG | + | chr7.1:7355149-7355168 | None:intergenic | 25.0% |
| ! | TTCTCTGCTATTAATATGTA+TGG | - | chr7.1:7355316-7355335 | MS.gene09842:CDS | 25.0% |
| !! | GAACTTAACTTTTTGTCTAT+TGG | + | chr7.1:7354929-7354948 | None:intergenic | 25.0% |
| !! | TAAGCTTAAGTTTAAGTTTG+TGG | - | chr7.1:7355456-7355475 | MS.gene09842:intron | 25.0% |
| !! | TAGAGATGTATCATTTTAGA+AGG | + | chr7.1:7355214-7355233 | None:intergenic | 25.0% |
| AAAAAGAAGGCAACAAAGAT+TGG | - | chr7.1:7355385-7355404 | MS.gene09842:CDS | 30.0% | |
| AATAAGGGATGGAAAAAAGA+AGG | - | chr7.1:7355372-7355391 | MS.gene09842:CDS | 30.0% | |
| ATAAAATGCTCAACAAAGGA+AGG | + | chr7.1:7354867-7354886 | None:intergenic | 30.0% | |
| ATTAATATGTATGGACCTAC+AGG | - | chr7.1:7355325-7355344 | MS.gene09842:CDS | 30.0% | |
| CAAATGTAATGAAGGTTTCT+AGG | - | chr7.1:7355491-7355510 | MS.gene09842:CDS | 30.0% | |
| TACTGCTTATATCATCAATG+AGG | - | chr7.1:7354745-7354764 | MS.gene09842:CDS | 30.0% | |
| TAGTACATCACATTTGTACT+CGG | + | chr7.1:7355353-7355372 | None:intergenic | 30.0% | |
| TGATCTTTATGAAGTTCTTG+GGG | - | chr7.1:7355028-7355047 | MS.gene09842:intron | 30.0% | |
| ! | ATCAACTTAAGGGAGTTTTA+TGG | - | chr7.1:7354988-7355007 | MS.gene09842:intron | 30.0% |
| ! | GTTGATAAAATGCTCAACAA+AGG | + | chr7.1:7354871-7354890 | None:intergenic | 30.0% |
| !! | ATCATTTTAGAAGGAGTTGT+TGG | + | chr7.1:7355205-7355224 | None:intergenic | 30.0% |
| ATATCTCATTTACAGCCGTA+TGG | - | chr7.1:7354831-7354850 | MS.gene09842:CDS | 35.0% | |
| TGTGATGTACTAATAAGGGA+TGG | - | chr7.1:7355361-7355380 | MS.gene09842:CDS | 35.0% | |
| ! | TGTAAAGACAATGGATTCCA+TGG | - | chr7.1:7355427-7355446 | MS.gene09842:intron | 35.0% |
| !! | ACTTAAGGGAGTTTTATGGT+TGG | - | chr7.1:7354992-7355011 | MS.gene09842:intron | 35.0% |
| ATGTCAACTCATTGCCATCA+TGG | - | chr7.1:7355091-7355110 | MS.gene09842:CDS | 40.0% | |
| CACTTACAAGCAAGGAATTG+AGG | - | chr7.1:7355116-7355135 | MS.gene09842:CDS | 40.0% | |
| GAACATCCACTTCTAACTGA+GGG | - | chr7.1:7354961-7354980 | MS.gene09842:intron | 40.0% | |
| GAAGGTCTAATCTCTCCATA+CGG | + | chr7.1:7354849-7354868 | None:intergenic | 40.0% | |
| GGTGATTACATTAGAAGCTC+AGG | - | chr7.1:7355292-7355311 | MS.gene09842:CDS | 40.0% | |
| TATGGCTGCAGGTTATGAAT+TGG | - | chr7.1:7355173-7355192 | MS.gene09842:CDS | 40.0% | |
| TCATAACCTGCAGCCATATA+AGG | + | chr7.1:7355171-7355190 | None:intergenic | 40.0% | |
| TGAACATCCACTTCTAACTG+AGG | - | chr7.1:7354960-7354979 | MS.gene09842:intron | 40.0% | |
| TGAGGGATGGATCAACTTAA+GGG | - | chr7.1:7354978-7354997 | MS.gene09842:intron | 40.0% | |
| TTAACTCCTTATATGGCTGC+AGG | - | chr7.1:7355162-7355181 | MS.gene09842:CDS | 40.0% | |
| TTCCAATTCACTGCATTTCG+TGG | - | chr7.1:7355060-7355079 | MS.gene09842:CDS | 40.0% | |
| ! | AAAGACAATGGATTCCATGG+TGG | - | chr7.1:7355430-7355449 | MS.gene09842:intron | 40.0% |
| ! | AAGACAATGGATTCCATGGT+GGG | - | chr7.1:7355431-7355450 | MS.gene09842:intron | 40.0% |
| !! | CAGACACTGAACATTGACTT+TGG | - | chr7.1:7355271-7355290 | MS.gene09842:CDS | 40.0% |
| AAGGCAACAAAGATTGGCAG+TGG | - | chr7.1:7355391-7355410 | MS.gene09842:intron | 45.0% | |
| ACTTAAGCTTATCCCCACCA+TGG | + | chr7.1:7355447-7355466 | None:intergenic | 45.0% | |
| ATCCACTTCTAACTGAGGGA+TGG | - | chr7.1:7354965-7354984 | MS.gene09842:intron | 45.0% | |
| ATCCATCCCTCAGTTAGAAG+TGG | + | chr7.1:7354970-7354989 | None:intergenic | 45.0% | |
| CAACAAAGATTGGCAGTGGA+TGG | - | chr7.1:7355395-7355414 | MS.gene09842:intron | 45.0% | |
| CTGAGGGATGGATCAACTTA+AGG | - | chr7.1:7354977-7354996 | MS.gene09842:intron | 45.0% | |
| GTCCACGAAATGCAGTGAAT+TGG | + | chr7.1:7355065-7355084 | None:intergenic | 45.0% | |
| TCATGGCACACTTACAAGCA+AGG | - | chr7.1:7355108-7355127 | MS.gene09842:CDS | 45.0% | |
| TGCTTGTAAGTGTGCCATGA+TGG | + | chr7.1:7355108-7355127 | None:intergenic | 45.0% | |
| ! | AGACAATGGATTCCATGGTG+GGG | - | chr7.1:7355432-7355451 | MS.gene09842:intron | 45.0% |
| !! | CATTTGTACTCGGTGCCTGT+AGG | + | chr7.1:7355343-7355362 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 7354732 | 7355519 | 7354732 | ID=MS.gene09842 |
| chr7.1 | mRNA | 7354732 | 7355519 | 7354732 | ID=MS.gene09842.t1;Parent=MS.gene09842 |
| chr7.1 | exon | 7355485 | 7355519 | 7355485 | ID=MS.gene09842.t1.exon1;Parent=MS.gene09842.t1 |
| chr7.1 | CDS | 7355485 | 7355519 | 7355485 | ID=cds.MS.gene09842.t1;Parent=MS.gene09842.t1 |
| chr7.1 | exon | 7355057 | 7355405 | 7355057 | ID=MS.gene09842.t1.exon2;Parent=MS.gene09842.t1 |
| chr7.1 | CDS | 7355057 | 7355405 | 7355057 | ID=cds.MS.gene09842.t1;Parent=MS.gene09842.t1 |
| chr7.1 | exon | 7354732 | 7354977 | 7354732 | ID=MS.gene09842.t1.exon3;Parent=MS.gene09842.t1 |
| chr7.1 | CDS | 7354732 | 7354977 | 7354732 | ID=cds.MS.gene09842.t1;Parent=MS.gene09842.t1 |
| Gene Sequence |
| Protein sequence |
