Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09878.t1 | XP_003625472.1 | 99.2 | 130 | 1 | 0 | 1 | 130 | 1 | 130 | 1.10E-64 | 255.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09878.t1 | F4JZT3 | 63.0 | 119 | 44 | 0 | 9 | 127 | 16 | 134 | 4.6e-35 | 148.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09878.t1 | G7KZK5 | 99.2 | 130 | 1 | 0 | 1 | 130 | 1 | 130 | 8.0e-65 | 255.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene09878.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09878.t1 | MTR_7g099500 | 99.231 | 130 | 1 | 0 | 1 | 130 | 1 | 130 | 2.67e-93 | 265 |
| MS.gene09878.t1 | MTR_8g063580 | 67.442 | 129 | 39 | 2 | 1 | 126 | 1 | 129 | 1.19e-54 | 168 |
| MS.gene09878.t1 | MTR_7g098910 | 59.200 | 125 | 50 | 1 | 5 | 129 | 16 | 139 | 9.52e-46 | 145 |
| MS.gene09878.t1 | MTR_6g008860 | 34.211 | 114 | 75 | 0 | 11 | 124 | 33 | 146 | 3.15e-18 | 75.9 |
| MS.gene09878.t1 | MTR_4g071650 | 34.211 | 114 | 75 | 0 | 11 | 124 | 33 | 146 | 3.15e-18 | 75.9 |
| MS.gene09878.t1 | MTR_7g098860 | 47.500 | 80 | 41 | 1 | 34 | 112 | 12 | 91 | 1.09e-15 | 67.8 |
| MS.gene09878.t1 | MTR_0022s0450 | 52.459 | 61 | 29 | 0 | 17 | 77 | 40 | 100 | 1.23e-14 | 65.1 |
| MS.gene09878.t1 | MTR_8g106150 | 27.820 | 133 | 79 | 1 | 14 | 129 | 621 | 753 | 1.72e-12 | 63.5 |
| MS.gene09878.t1 | MTR_8g106150 | 27.820 | 133 | 79 | 1 | 14 | 129 | 864 | 996 | 1.76e-12 | 63.5 |
| MS.gene09878.t1 | MTR_5g044100 | 43.284 | 67 | 38 | 0 | 14 | 80 | 155 | 221 | 2.01e-12 | 62.0 |
| MS.gene09878.t1 | MTR_5g097410 | 41.791 | 67 | 39 | 0 | 14 | 80 | 1077 | 1143 | 1.42e-11 | 60.8 |
| MS.gene09878.t1 | MTR_8g075340 | 30.597 | 134 | 72 | 3 | 11 | 123 | 1029 | 1162 | 2.20e-11 | 60.1 |
| MS.gene09878.t1 | MTR_5g022470 | 30.000 | 150 | 74 | 4 | 11 | 129 | 1050 | 1199 | 9.51e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09878.t1 | AT5G26594 | 63.025 | 119 | 44 | 0 | 9 | 127 | 16 | 134 | 1.25e-48 | 152 |
| MS.gene09878.t1 | AT3G04280 | 46.789 | 109 | 56 | 2 | 11 | 117 | 22 | 130 | 6.40e-26 | 95.1 |
| MS.gene09878.t1 | AT3G04280 | 46.789 | 109 | 56 | 2 | 11 | 117 | 22 | 130 | 6.40e-26 | 95.1 |
| MS.gene09878.t1 | AT3G04280 | 46.789 | 109 | 56 | 2 | 11 | 117 | 22 | 130 | 6.40e-26 | 95.1 |
| MS.gene09878.t1 | AT2G01830 | 30.657 | 137 | 82 | 2 | 2 | 125 | 922 | 1058 | 4.46e-13 | 65.1 |
| MS.gene09878.t1 | AT2G01830 | 30.657 | 137 | 82 | 2 | 2 | 125 | 933 | 1069 | 4.87e-13 | 65.1 |
| MS.gene09878.t1 | AT2G01830 | 30.657 | 137 | 82 | 2 | 2 | 125 | 910 | 1046 | 5.10e-13 | 65.1 |
| MS.gene09878.t1 | AT2G01830 | 30.657 | 137 | 82 | 2 | 2 | 125 | 910 | 1046 | 5.10e-13 | 65.1 |
| MS.gene09878.t1 | AT2G01830 | 30.657 | 137 | 82 | 2 | 2 | 125 | 910 | 1046 | 5.10e-13 | 65.1 |
| MS.gene09878.t1 | AT2G01830 | 30.657 | 137 | 82 | 2 | 2 | 125 | 910 | 1046 | 5.10e-13 | 65.1 |
| MS.gene09878.t1 | AT5G35750 | 28.889 | 135 | 74 | 2 | 14 | 126 | 1038 | 1172 | 1.58e-12 | 63.5 |
Find 31 sgRNAs with CRISPR-Local
Find 81 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAAAAGATGTTGAATAAATT+TGG | 0.144966 | 7.3:-12936573 | MS.gene09878:CDS |
| GCAATACTTAGTTCCATTCT+TGG | 0.228362 | 7.3:-12935223 | MS.gene09878:CDS |
| TATCCATGTCCATGAGAATT+AGG | 0.311699 | 7.3:+12936481 | None:intergenic |
| TGGATTGATCTTGCCAAGAA+TGG | 0.418939 | 7.3:+12935210 | None:intergenic |
| CACAACCAGTGCTGTTAGCT+TGG | 0.421611 | 7.3:+12936626 | None:intergenic |
| AAAATTCATGGAAGCTGGAT+TGG | 0.421768 | 7.3:-12935275 | MS.gene09878:CDS |
| TGGTGTGTCATCACGCTCTA+TGG | 0.423024 | 7.3:-12935311 | MS.gene09878:CDS |
| AGATGAAATACTAAAATTCA+TGG | 0.428022 | 7.3:-12935287 | MS.gene09878:CDS |
| ACTAAGTATTGCATTGTTCA+AGG | 0.440010 | 7.3:+12935234 | None:intergenic |
| AAGAAGCAGTGGACATTCAT+TGG | 0.445266 | 7.3:-12936521 | MS.gene09878:CDS |
| ATACTAAAATTCATGGAAGC+TGG | 0.448559 | 7.3:-12935280 | MS.gene09878:CDS |
| GGTATTTGTAGCACAATTGT+TGG | 0.475026 | 7.3:-12935331 | MS.gene09878:CDS |
| AAAATTTGACCTAATTCTCA+TGG | 0.477878 | 7.3:-12936490 | MS.gene09878:CDS |
| AAAAGTCAAGGTGTGGAGAA+TGG | 0.478580 | 7.3:-12936546 | MS.gene09878:CDS |
| ACAAAGGAACTTCGTGCAAT+GGG | 0.482431 | 7.3:-12935352 | MS.gene09878:CDS |
| CAAGCTAACAGCACTGGTTG+TGG | 0.482562 | 7.3:-12936625 | MS.gene09878:CDS |
| ACCTCAATGCCATTCATGAT+AGG | 0.492567 | 7.3:+12936456 | None:intergenic |
| CCTTGCATCCATCACACAGT+TGG | 0.511788 | 7.3:+12935190 | None:intergenic |
| AACAAAGGAACTTCGTGCAA+TGG | 0.518430 | 7.3:-12935353 | MS.gene09878:CDS |
| ATGGATATGCCTATCATGAA+TGG | 0.542735 | 7.3:-12936465 | MS.gene09878:CDS |
| CTAAGTATTGCATTGTTCAA+GGG | 0.546876 | 7.3:+12935235 | None:intergenic |
| CAGTGGACATTCATTGGCGT+GGG | 0.559565 | 7.3:-12936515 | MS.gene09878:CDS |
| GCCTATCATGAATGGCATTG+AGG | 0.559799 | 7.3:-12936457 | MS.gene09878:intron |
| TGTATCCAAGCTAACAGCAC+TGG | 0.575373 | 7.3:-12936631 | MS.gene09878:CDS |
| TGTGTTTATATAGGCAACAA+AGG | 0.581181 | 7.3:-12935368 | MS.gene09878:intron |
| AGATCAATCCAACTGTGTGA+TGG | 0.583988 | 7.3:-12935198 | None:intergenic |
| TGACCTAATTCTCATGGACA+TGG | 0.592027 | 7.3:-12936484 | MS.gene09878:CDS |
| GCAGTGGACATTCATTGGCG+TGG | 0.597898 | 7.3:-12936516 | MS.gene09878:CDS |
| AAATTTGGAGTCAAAAGTCA+AGG | 0.637368 | 7.3:-12936558 | MS.gene09878:CDS |
| TGGAGTCAAAAGTCAAGGTG+TGG | 0.637883 | 7.3:-12936553 | MS.gene09878:CDS |
| GGAGAATGGAAAAGAAGCAG+TGG | 0.653869 | 7.3:-12936532 | MS.gene09878:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATATAAATATAAATAAAAA+AGG | + | chr7.3:12936452-12936471 | None:intergenic | 0.0% |
| !! | ACTAAAATTAACTATAAAAT+TGG | + | chr7.3:12936177-12936196 | None:intergenic | 10.0% |
| !! | CATATAAAAATATATAAACT+TGG | + | chr7.3:12935643-12935662 | None:intergenic | 10.0% |
| !! | TATAAAAATATATAAACTTG+GGG | + | chr7.3:12935641-12935660 | None:intergenic | 10.0% |
| !!! | AAAAAAATATTTTACTTTTG+AGG | + | chr7.3:12936007-12936026 | None:intergenic | 10.0% |
| !!! | AATTTTATAGTTAATTTTAG+TGG | - | chr7.3:12936176-12936195 | MS.gene09878:intron | 10.0% |
| !! | AAAATTAACTATAAAATTGG+AGG | + | chr7.3:12936174-12936193 | None:intergenic | 15.0% |
| !! | AAATTAACTATAAAATTGGA+GGG | + | chr7.3:12936173-12936192 | None:intergenic | 15.0% |
| !!! | ATATTTATATAGTTTGTTAG+AGG | - | chr7.3:12935858-12935877 | MS.gene09878:intron | 15.0% |
| !! | AGATGAAATACTAAAATTCA+TGG | - | chr7.3:12936565-12936584 | MS.gene09878:CDS | 20.0% |
| !! | CAAAAGATGTTGAATAAATT+TGG | - | chr7.3:12935279-12935298 | MS.gene09878:CDS | 20.0% |
| !! | CTCTATAATTAATTAAGTAC+AGG | + | chr7.3:12935425-12935444 | None:intergenic | 20.0% |
| !! | GAAGAAAATGAAAATACTTA+TGG | - | chr7.3:12935736-12935755 | MS.gene09878:intron | 20.0% |
| !! | TGATTATGGTCTATATAATT+TGG | - | chr7.3:12935661-12935680 | MS.gene09878:intron | 20.0% |
| ! | AAAATTTGACCTAATTCTCA+TGG | - | chr7.3:12935362-12935381 | MS.gene09878:intron | 25.0% |
| ! | ACTAAAGGAATTTAAGAATG+AGG | + | chr7.3:12935921-12935940 | None:intergenic | 25.0% |
| ! | GTCAAAAAGTTGTAATAAAG+TGG | - | chr7.3:12935581-12935600 | MS.gene09878:intron | 25.0% |
| ! | TAGTGTACTATTATTGTTCA+TGG | - | chr7.3:12935714-12935733 | MS.gene09878:intron | 25.0% |
| ! | TGATATATGTACAACCATTT+GGG | - | chr7.3:12935448-12935467 | MS.gene09878:intron | 25.0% |
| ! | TGTGTGTTATGTGTTTATAT+AGG | - | chr7.3:12936475-12936494 | MS.gene09878:CDS | 25.0% |
| ! | TTAAATTCCTTTAGTGTTCA+GGG | - | chr7.3:12935926-12935945 | MS.gene09878:intron | 25.0% |
| ! | TTAGAGGAAGGAAAAATTAT+TGG | - | chr7.3:12935874-12935893 | MS.gene09878:intron | 25.0% |
| !! | ACATCTTTTGATGAATCTTT+CGG | + | chr7.3:12935269-12935288 | None:intergenic | 25.0% |
| !! | TTCATTTTCTCTCTTTTCAT+TGG | - | chr7.3:12935488-12935507 | MS.gene09878:intron | 25.0% |
| !!! | CTCATTTGATTTTGTTACTA+TGG | - | chr7.3:12936065-12936084 | MS.gene09878:intron | 25.0% |
| !!! | GATAGTTTTATTGAGAAAGA+AGG | + | chr7.3:12936342-12936361 | None:intergenic | 25.0% |
| !!! | TTATATAGTTTGTTAGAGGA+AGG | - | chr7.3:12935862-12935881 | MS.gene09878:intron | 25.0% |
| AAAGAAGGAATTAGTACAAG+TGG | + | chr7.3:12936327-12936346 | None:intergenic | 30.0% | |
| AGGAAAATATGTTCACTCAT+TGG | - | chr7.3:12936287-12936306 | MS.gene09878:intron | 30.0% | |
| ATACTAAAATTCATGGAAGC+TGG | - | chr7.3:12936572-12936591 | MS.gene09878:CDS | 30.0% | |
| CTTAAATTCCTTTAGTGTTC+AGG | - | chr7.3:12935925-12935944 | MS.gene09878:intron | 30.0% | |
| GTGATATATGTACAACCATT+TGG | - | chr7.3:12935447-12935466 | MS.gene09878:intron | 30.0% | |
| TGAGTGAACTTTATAATACG+AGG | + | chr7.3:12936049-12936068 | None:intergenic | 30.0% | |
| TGGTAACAAATATCGAATGT+TGG | - | chr7.3:12935894-12935913 | MS.gene09878:intron | 30.0% | |
| TGTGTTTATATAGGCAACAA+AGG | - | chr7.3:12936484-12936503 | MS.gene09878:CDS | 30.0% | |
| TTAAGTCTCTCTAGATGTTT+AGG | - | chr7.3:12936219-12936238 | MS.gene09878:intron | 30.0% | |
| ! | AAATTTGGAGTCAAAAGTCA+AGG | - | chr7.3:12935294-12935313 | MS.gene09878:CDS | 30.0% |
| ! | ACTAAGTATTGCATTGTTCA+AGG | + | chr7.3:12936621-12936640 | None:intergenic | 30.0% |
| ! | CTAAGTATTGCATTGTTCAA+GGG | + | chr7.3:12936620-12936639 | None:intergenic | 30.0% |
| AAAATTCATGGAAGCTGGAT+TGG | - | chr7.3:12936577-12936596 | MS.gene09878:CDS | 35.0% | |
| AAAGTTGTGTCTTTACTTGC+AGG | + | chr7.3:12935965-12935984 | None:intergenic | 35.0% | |
| AAATACTTATGGCCCAACAT+TGG | - | chr7.3:12935747-12935766 | MS.gene09878:intron | 35.0% | |
| AAGTTGTGTCTTTACTTGCA+GGG | + | chr7.3:12935964-12935983 | None:intergenic | 35.0% | |
| ACAACTAAGCTACATCTTCA+AGG | - | chr7.3:12936260-12936279 | MS.gene09878:intron | 35.0% | |
| ATGGATATGCCTATCATGAA+TGG | - | chr7.3:12935387-12935406 | MS.gene09878:intron | 35.0% | |
| CAACTAAGCTACATCTTCAA+GGG | - | chr7.3:12936261-12936280 | MS.gene09878:intron | 35.0% | |
| GAAGATGTAGCTTAGTTGTA+TGG | + | chr7.3:12936259-12936278 | None:intergenic | 35.0% | |
| GCAATACTTAGTTCCATTCT+TGG | - | chr7.3:12936629-12936648 | MS.gene09878:CDS | 35.0% | |
| GGTATTTGTAGCACAATTGT+TGG | - | chr7.3:12936521-12936540 | MS.gene09878:CDS | 35.0% | |
| TATCCATGTCCATGAGAATT+AGG | + | chr7.3:12935374-12935393 | None:intergenic | 35.0% | |
| TGCAAGTAAAGACACAACTT+TGG | - | chr7.3:12935964-12935983 | MS.gene09878:intron | 35.0% | |
| TGTCATCAAATTGTCCCAAA+TGG | + | chr7.3:12935465-12935484 | None:intergenic | 35.0% | |
| ! | AAAGTACCTTCACCAATGTT+GGG | + | chr7.3:12935762-12935781 | None:intergenic | 35.0% |
| ! | AGCTTGGATACAAAGTTTTG+AGG | + | chr7.3:12935213-12935232 | None:intergenic | 35.0% |
| !! | AAAAGTACCTTCACCAATGT+TGG | + | chr7.3:12935763-12935782 | None:intergenic | 35.0% |
| AAAAGTCAAGGTGTGGAGAA+TGG | - | chr7.3:12935306-12935325 | MS.gene09878:CDS | 40.0% | |
| AACAAAGGAACTTCGTGCAA+TGG | - | chr7.3:12936499-12936518 | MS.gene09878:CDS | 40.0% | |
| AAGAAGCAGTGGACATTCAT+TGG | - | chr7.3:12935331-12935350 | MS.gene09878:CDS | 40.0% | |
| ACAAAGGAACTTCGTGCAAT+GGG | - | chr7.3:12936500-12936519 | MS.gene09878:CDS | 40.0% | |
| ACCTCAATGCCATTCATGAT+AGG | + | chr7.3:12935399-12935418 | None:intergenic | 40.0% | |
| TGACCTAATTCTCATGGACA+TGG | - | chr7.3:12935368-12935387 | MS.gene09878:intron | 40.0% | |
| TGGATTGATCTTGCCAAGAA+TGG | + | chr7.3:12936645-12936664 | None:intergenic | 40.0% | |
| CTAAGCTACATCTTCAAGGG+AGG | - | chr7.3:12936264-12936283 | MS.gene09878:intron | 45.0% | |
| GCCTATCATGAATGGCATTG+AGG | - | chr7.3:12935395-12935414 | MS.gene09878:intron | 45.0% | |
| GGAGAATGGAAAAGAAGCAG+TGG | - | chr7.3:12935320-12935339 | MS.gene09878:CDS | 45.0% | |
| GGATCAACCCTGAACACTAA+AGG | + | chr7.3:12935936-12935955 | None:intergenic | 45.0% | |
| TGGAGTCAAAAGTCAAGGTG+TGG | - | chr7.3:12935299-12935318 | MS.gene09878:CDS | 45.0% | |
| TGTATCCAAGCTAACAGCAC+TGG | - | chr7.3:12935221-12935240 | MS.gene09878:CDS | 45.0% | |
| TTATGGCCCAACATTGGTGA+AGG | - | chr7.3:12935753-12935772 | MS.gene09878:intron | 45.0% | |
| !! | AGAAATAAATATTTATTTAT+AGG | - | chr7.3:12935826-12935845 | MS.gene09878:intron | 5.0% |
| !! | ATATAAAAATATATAAACTT+GGG | + | chr7.3:12935642-12935661 | None:intergenic | 5.0% |
| !!! | AATAAATATTTATTTTTTGA+AGG | - | chr7.3:12935799-12935818 | MS.gene09878:intron | 5.0% |
| !!! | TATATATTTTTATATGATTA+TGG | - | chr7.3:12935647-12935666 | MS.gene09878:intron | 5.0% |
| !!! | TTTTTAATAGTATTAATTTT+AGG | - | chr7.3:12936023-12936042 | MS.gene09878:intron | 5.0% |
| AGCTACATCTTCAAGGGAGG+AGG | - | chr7.3:12936267-12936286 | MS.gene09878:intron | 50.0% | |
| CAGTGGACATTCATTGGCGT+GGG | - | chr7.3:12935337-12935356 | MS.gene09878:CDS | 50.0% | |
| GTCTTTACTTGCAGGGTCGT+TGG | + | chr7.3:12935957-12935976 | None:intergenic | 50.0% | |
| TGGTGTGTCATCACGCTCTA+TGG | - | chr7.3:12936541-12936560 | MS.gene09878:CDS | 50.0% | |
| ! | CACAACCAGTGCTGTTAGCT+TGG | + | chr7.3:12935229-12935248 | None:intergenic | 50.0% |
| !! | CAAGCTAACAGCACTGGTTG+TGG | - | chr7.3:12935227-12935246 | MS.gene09878:CDS | 50.0% |
| GCAGTGGACATTCATTGGCG+TGG | - | chr7.3:12935336-12935355 | MS.gene09878:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 12935201 | 12936673 | 12935201 | ID=MS.gene09878 |
| chr7.3 | mRNA | 12935201 | 12936673 | 12935201 | ID=MS.gene09878.t1;Parent=MS.gene09878 |
| chr7.3 | exon | 12936458 | 12936673 | 12936458 | ID=MS.gene09878.t1.exon1;Parent=MS.gene09878.t1 |
| chr7.3 | CDS | 12936458 | 12936673 | 12936458 | ID=cds.MS.gene09878.t1;Parent=MS.gene09878.t1 |
| chr7.3 | exon | 12935201 | 12935377 | 12935201 | ID=MS.gene09878.t1.exon2;Parent=MS.gene09878.t1 |
| chr7.3 | CDS | 12935201 | 12935377 | 12935201 | ID=cds.MS.gene09878.t1;Parent=MS.gene09878.t1 |
| Gene Sequence |
| Protein sequence |
