Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09913.t1 | AES81629.1 | 94.2 | 86 | 5 | 0 | 38 | 123 | 11 | 96 | 1.20E-37 | 166 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09913.t1 | F4JZT3 | 39.2 | 120 | 69 | 2 | 16 | 135 | 18 | 133 | 1.1e-13 | 77.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09913.t1 | G7KYG8 | 94.2 | 86 | 5 | 0 | 38 | 123 | 11 | 96 | 8.9e-38 | 166.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene09913.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09913.t1 | MTR_7g098860 | 94.186 | 86 | 5 | 0 | 38 | 123 | 11 | 96 | 1.53e-54 | 166 |
| MS.gene09913.t1 | MTR_7g098910 | 47.500 | 120 | 58 | 3 | 16 | 135 | 22 | 136 | 2.47e-25 | 94.0 |
| MS.gene09913.t1 | MTR_7g099500 | 43.902 | 123 | 65 | 2 | 16 | 138 | 11 | 129 | 1.22e-24 | 92.0 |
| MS.gene09913.t1 | MTR_8g063580 | 45.545 | 101 | 52 | 2 | 16 | 114 | 12 | 111 | 9.56e-18 | 74.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene09913.t1 | AT5G26594 | 39.167 | 120 | 69 | 2 | 16 | 135 | 18 | 133 | 4.02e-19 | 78.2 |
| MS.gene09913.t1 | AT3G04280 | 41.414 | 99 | 57 | 1 | 16 | 113 | 22 | 120 | 5.46e-15 | 67.4 |
| MS.gene09913.t1 | AT3G04280 | 41.414 | 99 | 57 | 1 | 16 | 113 | 22 | 120 | 5.46e-15 | 67.4 |
| MS.gene09913.t1 | AT3G04280 | 41.414 | 99 | 57 | 1 | 16 | 113 | 22 | 120 | 5.46e-15 | 67.4 |
Find 32 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGCGGGACTCTGTGATGATA+TGG | 0.319136 | 7.1:-9918527 | None:intergenic |
| GCCCGTCATGGATGGAATTA+CGG | 0.406314 | 7.1:+9918721 | MS.gene09913:CDS |
| CTGCATATTCATCATTAGTA+AGG | 0.410681 | 7.1:-9919017 | None:intergenic |
| AGAGATATCTTGACATTACT+TGG | 0.437895 | 7.1:+9918602 | MS.gene09913:CDS |
| GAATACCCTGGCCAGATTGT+TGG | 0.444431 | 7.1:+9918982 | MS.gene09913:CDS |
| AACCGTGCCAATGAGGTCTA+AGG | 0.458884 | 7.1:-9918660 | None:intergenic |
| TATGCAGAATTTCGCAATGC+AGG | 0.461823 | 7.1:+9919033 | MS.gene09913:CDS |
| GACGTGAGACACCAACAATC+TGG | 0.490889 | 7.1:-9918993 | None:intergenic |
| GATGATCTACATCATGAAGA+TGG | 0.493143 | 7.1:+9919060 | MS.gene09913:CDS |
| CCCGCGTGGATTAAAAGTAC+TGG | 0.511605 | 7.1:+9918544 | MS.gene09913:CDS |
| GAGACACCAACAATCTGGCC+AGG | 0.513873 | 7.1:-9918988 | None:intergenic |
| AAAGAAGCCTTAGACCTCAT+TGG | 0.516268 | 7.1:+9918653 | MS.gene09913:CDS |
| AGGATCATGCCCGTCATGGA+TGG | 0.527719 | 7.1:+9918713 | MS.gene09913:CDS |
| TATCTTGACATTACTTGGTG+TGG | 0.530817 | 7.1:+9918607 | MS.gene09913:CDS |
| TTTCAACATATGATAGTGCA+AGG | 0.532595 | 7.1:-9919088 | None:intergenic |
| AAAAGGTCATGCTAGTTGAC+AGG | 0.534345 | 7.1:-9919132 | None:intergenic |
| GGCTAGGATCATGCCCGTCA+TGG | 0.548604 | 7.1:+9918709 | MS.gene09913:CDS |
| ACCGTAATTCCATCCATGAC+GGG | 0.549235 | 7.1:-9918722 | None:intergenic |
| TGGATTAAAAGTACTGGTTG+TGG | 0.556379 | 7.1:+9918550 | MS.gene09913:CDS |
| ATGCAGAATTTCGCAATGCA+GGG | 0.581025 | 7.1:+9919034 | MS.gene09913:CDS |
| TGATCCTAGCCATGAGAATG+AGG | 0.586084 | 7.1:-9918697 | None:intergenic |
| TAAATTCGACCTCATTCTCA+TGG | 0.588335 | 7.1:+9918688 | MS.gene09913:CDS |
| TCGACCTCATTCTCATGGCT+AGG | 0.592585 | 7.1:+9918693 | MS.gene09913:CDS |
| AACCGTAATTCCATCCATGA+CGG | 0.593130 | 7.1:-9918723 | None:intergenic |
| TACGTTCAACCGTGCCAATG+AGG | 0.601575 | 7.1:-9918667 | None:intergenic |
| AGCCTTAGACCTCATTGGCA+CGG | 0.608494 | 7.1:+9918658 | MS.gene09913:CDS |
| AGATGATGATCTTGTTCGAA+TGG | 0.623757 | 7.1:+9918574 | MS.gene09913:CDS |
| TTCAACATATGATAGTGCAA+GGG | 0.630612 | 7.1:-9919087 | None:intergenic |
| AGACACCAACAATCTGGCCA+GGG | 0.657108 | 7.1:-9918987 | None:intergenic |
| GGGACTCTGTGATGATATGG+AGG | 0.663830 | 7.1:-9918524 | None:intergenic |
| CTGCGCGATTTAGAATACCC+TGG | 0.695519 | 7.1:+9918970 | MS.gene09913:CDS |
| TATCATCACAGAGTCCCGCG+TGG | 0.741838 | 7.1:+9918530 | MS.gene09913:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTATGAATGATTATATGCT+AGG | - | chr7.1:9918899-9918918 | None:intergenic | 20.0% |
| !! | TAATTACATACATACATACA+CGG | + | chr7.1:9918757-9918776 | MS.gene09913:intron | 20.0% |
| !!! | AATTGCTTTAAACTAATTCA+TGG | - | chr7.1:9918795-9918814 | None:intergenic | 20.0% |
| !!! | ATTGCTTTAAACTAATTCAT+GGG | - | chr7.1:9918794-9918813 | None:intergenic | 20.0% |
| !!! | GTTGAAACTAATTTTGAAAA+GGG | + | chr7.1:9919104-9919123 | MS.gene09913:CDS | 20.0% |
| !!! | TGTTGAAACTAATTTTGAAA+AGG | + | chr7.1:9919103-9919122 | MS.gene09913:CDS | 20.0% |
| AGAGATATCTTGACATTACT+TGG | + | chr7.1:9918602-9918621 | MS.gene09913:CDS | 30.0% | |
| ATGAACAGAAAGAAAATGCA+TGG | + | chr7.1:9918857-9918876 | MS.gene09913:intron | 30.0% | |
| CTGCATATTCATCATTAGTA+AGG | - | chr7.1:9919020-9919039 | None:intergenic | 30.0% | |
| TGAACAGAAAGAAAATGCAT+GGG | + | chr7.1:9918858-9918877 | MS.gene09913:intron | 30.0% | |
| ! | TTCAACATATGATAGTGCAA+GGG | - | chr7.1:9919090-9919109 | None:intergenic | 30.0% |
| ! | TTTCAACATATGATAGTGCA+AGG | - | chr7.1:9919091-9919110 | None:intergenic | 30.0% |
| AGATGATGATCTTGTTCGAA+TGG | + | chr7.1:9918574-9918593 | MS.gene09913:CDS | 35.0% | |
| GAACAGAAAGAAAATGCATG+GGG | + | chr7.1:9918859-9918878 | MS.gene09913:intron | 35.0% | |
| GATGATCTACATCATGAAGA+TGG | + | chr7.1:9919060-9919079 | MS.gene09913:CDS | 35.0% | |
| TAAATTCGACCTCATTCTCA+TGG | + | chr7.1:9918688-9918707 | MS.gene09913:CDS | 35.0% | |
| ! | TATCTTGACATTACTTGGTG+TGG | + | chr7.1:9918607-9918626 | MS.gene09913:CDS | 35.0% |
| !! | TGGATTAAAAGTACTGGTTG+TGG | + | chr7.1:9918550-9918569 | MS.gene09913:CDS | 35.0% |
| AAAGAAGCCTTAGACCTCAT+TGG | + | chr7.1:9918653-9918672 | MS.gene09913:CDS | 40.0% | |
| AACCGTAATTCCATCCATGA+CGG | - | chr7.1:9918726-9918745 | None:intergenic | 40.0% | |
| ATGCAGAATTTCGCAATGCA+GGG | + | chr7.1:9919034-9919053 | MS.gene09913:CDS | 40.0% | |
| GAAACTCACGCAGTCAAAAA+TGG | + | chr7.1:9918629-9918648 | MS.gene09913:CDS | 40.0% | |
| TATGCAGAATTTCGCAATGC+AGG | + | chr7.1:9919033-9919052 | MS.gene09913:CDS | 40.0% | |
| ! | ACCAGTACTTTTAATCCACG+CGG | - | chr7.1:9918548-9918567 | None:intergenic | 40.0% |
| ACCGTAATTCCATCCATGAC+GGG | - | chr7.1:9918725-9918744 | None:intergenic | 45.0% | |
| CATTCATATGTGTGCACTGC+AGG | + | chr7.1:9918936-9918955 | MS.gene09913:intron | 45.0% | |
| GTCATGGATGGAATTACGGT+TGG | + | chr7.1:9918725-9918744 | MS.gene09913:intron | 45.0% | |
| TGATCCTAGCCATGAGAATG+AGG | - | chr7.1:9918700-9918719 | None:intergenic | 45.0% | |
| ! | CCAGTACTTTTAATCCACGC+GGG | - | chr7.1:9918547-9918566 | None:intergenic | 45.0% |
| AACCGTGCCAATGAGGTCTA+AGG | - | chr7.1:9918663-9918682 | None:intergenic | 50.0% | |
| AGACACCAACAATCTGGCCA+GGG | - | chr7.1:9918990-9919009 | None:intergenic | 50.0% | |
| AGCCTTAGACCTCATTGGCA+CGG | + | chr7.1:9918658-9918677 | MS.gene09913:CDS | 50.0% | |
| GAATACCCTGGCCAGATTGT+TGG | + | chr7.1:9918982-9919001 | MS.gene09913:CDS | 50.0% | |
| GACGTGAGACACCAACAATC+TGG | - | chr7.1:9918996-9919015 | None:intergenic | 50.0% | |
| GCCCGTCATGGATGGAATTA+CGG | + | chr7.1:9918721-9918740 | MS.gene09913:CDS | 50.0% | |
| GGGACTCTGTGATGATATGG+AGG | - | chr7.1:9918527-9918546 | None:intergenic | 50.0% | |
| TACGTTCAACCGTGCCAATG+AGG | - | chr7.1:9918670-9918689 | None:intergenic | 50.0% | |
| TCGACCTCATTCTCATGGCT+AGG | + | chr7.1:9918693-9918712 | MS.gene09913:CDS | 50.0% | |
| ! | CCCGCGTGGATTAAAAGTAC+TGG | + | chr7.1:9918544-9918563 | MS.gene09913:CDS | 50.0% |
| ! | CTGCGCGATTTAGAATACCC+TGG | + | chr7.1:9918970-9918989 | MS.gene09913:CDS | 50.0% |
| AGGATCATGCCCGTCATGGA+TGG | + | chr7.1:9918713-9918732 | MS.gene09913:CDS | 55.0% | |
| CGCGGGACTCTGTGATGATA+TGG | - | chr7.1:9918530-9918549 | None:intergenic | 55.0% | |
| GAGACACCAACAATCTGGCC+AGG | - | chr7.1:9918991-9919010 | None:intergenic | 55.0% | |
| TATCATCACAGAGTCCCGCG+TGG | + | chr7.1:9918530-9918549 | MS.gene09913:CDS | 55.0% | |
| GGCTAGGATCATGCCCGTCA+TGG | + | chr7.1:9918709-9918728 | MS.gene09913:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.1 | gene | 9918509 | 9919143 | 9918509 | ID=MS.gene09913 |
| chr7.1 | mRNA | 9918509 | 9919143 | 9918509 | ID=MS.gene09913.t1;Parent=MS.gene09913 |
| chr7.1 | exon | 9918509 | 9918742 | 9918509 | ID=MS.gene09913.t1.exon1;Parent=MS.gene09913.t1 |
| chr7.1 | CDS | 9918509 | 9918742 | 9918509 | ID=cds.MS.gene09913.t1;Parent=MS.gene09913.t1 |
| chr7.1 | exon | 9918958 | 9919143 | 9918958 | ID=MS.gene09913.t1.exon2;Parent=MS.gene09913.t1 |
| chr7.1 | CDS | 9918958 | 9919143 | 9918958 | ID=cds.MS.gene09913.t1;Parent=MS.gene09913.t1 |
| Gene Sequence |
| Protein sequence |
