AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20274

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20274.t1 MTR_0558s0020 86.824 296 38 1 1 295 1 296 0.0 538
MS.gene20274.t1 MTR_1g083640 82.394 284 34 1 1 268 1 284 3.50e-170 486
MS.gene20274.t1 MTR_8g446020 73.220 295 40 1 1 295 1 256 6.68e-150 433
MS.gene20274.t1 MTR_2g014100 81.633 245 36 1 1 236 1 245 1.12e-140 409
MS.gene20274.t1 MTR_6g090505 82.700 237 41 0 65 301 1 237 5.75e-140 412
MS.gene20274.t1 MTR_6g090505 67.647 102 6 2 396 495 230 306 5.29e-34 133
MS.gene20274.t1 MTR_6g090505 80.488 41 8 0 574 614 322 362 5.74e-13 71.2
MS.gene20274.t1 MTR_0337s0040 71.538 260 34 2 1 259 1 221 1.01e-121 360
MS.gene20274.t1 MTR_3g008240 83.744 203 32 1 1 202 1 203 9.17e-117 346
MS.gene20274.t1 MTR_4g040430 82.178 202 32 1 1 202 1 198 4.03e-114 339
MS.gene20274.t1 MTR_5g071590 72.778 180 36 2 1 167 1 180 1.07e-87 271
MS.gene20274.t1 MTR_1g055240 87.324 142 18 0 91 232 3 144 1.49e-85 264
MS.gene20274.t1 MTR_0019s0290 80.921 152 29 0 82 233 9 160 2.70e-83 258
MS.gene20274.t1 MTR_0337s0060 67.442 172 16 1 447 618 21 152 1.72e-71 231
MS.gene20274.t1 MTR_1281s0010 80.734 109 21 0 19 127 1 109 4.18e-57 188
MS.gene20274.t1 MTR_6g051425 32.075 318 185 6 40 341 22 324 1.46e-40 151
MS.gene20274.t1 MTR_4g008820 70.000 100 29 1 1 99 125 224 5.93e-32 132
MS.gene20274.t1 MTR_8g046060 77.273 88 11 2 1 79 91 178 3.08e-31 121
MS.gene20274.t1 MTR_1g070355 26.543 324 217 6 41 362 80 384 9.05e-19 90.5
MS.gene20274.t1 MTR_4g133495 57.895 57 24 0 567 623 80 136 4.61e-15 76.3
MS.gene20274.t1 MTR_4g133340 57.895 57 24 0 567 623 80 136 4.61e-15 76.3
MS.gene20274.t1 MTR_4g035820 23.103 290 190 7 17 299 61 324 1.15e-14 77.8
MS.gene20274.t1 MTR_0402s0040 24.865 185 120 4 49 233 40 205 2.74e-14 76.3
MS.gene20274.t1 MTR_0337s0050 75.000 36 9 0 259 294 14 49 2.83e-13 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20274.t1 AT4G38180 26.342 596 377 11 32 625 70 605 7.80e-60 214
MS.gene20274.t1 AT4G15090 25.214 583 361 13 50 625 66 580 1.81e-46 176
MS.gene20274.t1 AT4G15090 25.214 583 361 13 50 625 66 580 1.81e-46 176
MS.gene20274.t1 AT4G15090 25.214 583 361 13 50 625 66 580 1.81e-46 176
MS.gene20274.t1 AT4G15090 25.214 583 361 13 50 625 66 580 1.81e-46 176
MS.gene20274.t1 AT4G15090 25.214 583 361 13 50 625 66 580 1.81e-46 176
MS.gene20274.t1 AT3G06250 24.456 597 336 14 34 625 189 675 7.72e-44 168
MS.gene20274.t1 AT3G06250 24.456 597 336 14 34 625 189 675 7.72e-44 168
MS.gene20274.t1 AT3G06250 24.456 597 336 14 34 625 189 675 7.72e-44 168
MS.gene20274.t1 AT1G76320 24.742 582 354 15 50 625 12 515 1.74e-41 160
MS.gene20274.t1 AT1G76320 24.742 582 354 15 50 625 12 515 3.97e-41 159
MS.gene20274.t1 AT1G76320 24.742 582 354 15 50 625 12 515 3.97e-41 159
MS.gene20274.t1 AT1G76320 24.742 582 354 15 50 625 12 515 4.52e-41 159
MS.gene20274.t1 AT3G22170 22.781 597 380 13 34 625 69 589 2.41e-40 158
MS.gene20274.t1 AT3G22170 22.781 597 380 13 34 625 69 589 2.41e-40 158
MS.gene20274.t1 AT2G27110 25.447 503 311 12 127 625 108 550 1.36e-39 155
MS.gene20274.t1 AT2G27110 25.447 503 311 12 127 625 108 550 1.36e-39 155
MS.gene20274.t1 AT5G18960 23.810 609 348 17 24 625 200 699 1.18e-38 152
MS.gene20274.t1 AT5G18960 23.810 609 348 17 24 625 200 699 1.18e-38 152
MS.gene20274.t1 AT2G27110 26.506 415 258 8 213 625 36 405 2.26e-38 151
MS.gene20274.t1 AT4G38170 28.425 292 197 5 235 523 24 306 2.48e-38 149
MS.gene20274.t1 AT1G10240 25.085 590 356 19 49 625 62 578 5.30e-38 150
MS.gene20274.t1 AT1G10240 25.085 590 356 19 49 625 62 578 5.30e-38 150
MS.gene20274.t1 AT5G28530 25.096 522 338 14 40 540 60 549 3.59e-37 147
MS.gene20274.t1 AT5G28530 24.904 522 335 15 40 540 60 545 1.97e-36 145
MS.gene20274.t1 AT5G28530 24.904 522 335 15 40 540 60 545 1.97e-36 145
MS.gene20274.t1 AT2G32250 23.259 632 363 18 1 625 8 524 5.54e-35 141
MS.gene20274.t1 AT2G32250 23.259 632 363 18 1 625 8 524 6.30e-35 141
MS.gene20274.t1 AT2G32250 23.259 632 363 18 1 625 8 524 6.30e-35 141
MS.gene20274.t1 AT2G32250 23.418 632 362 19 1 625 8 524 7.25e-35 141
MS.gene20274.t1 AT2G32250 23.418 632 362 19 1 625 8 524 7.25e-35 141
MS.gene20274.t1 AT2G32250 23.418 632 362 19 1 625 8 524 7.25e-35 141
MS.gene20274.t1 AT2G32250 23.671 583 327 17 50 625 12 483 2.98e-34 139
MS.gene20274.t1 AT2G32250 23.671 583 327 17 50 625 12 483 3.07e-34 139
MS.gene20274.t1 AT1G80010 24.847 491 318 16 41 514 101 557 2.61e-31 130
MS.gene20274.t1 AT4G19990 22.521 595 360 14 41 625 27 530 7.29e-30 125
MS.gene20274.t1 AT4G19990 22.521 595 360 14 41 625 27 530 7.29e-30 125
MS.gene20274.t1 AT4G19990 22.521 595 360 14 41 625 41 544 8.60e-30 125
MS.gene20274.t1 AT4G19990 22.521 595 360 14 41 625 27 530 8.86e-30 125
MS.gene20274.t1 AT4G19990 22.521 595 360 14 41 625 27 530 1.23e-29 125
MS.gene20274.t1 AT4G19990 22.521 595 360 14 41 625 27 530 1.23e-29 125
MS.gene20274.t1 AT1G52520 22.702 533 359 14 41 557 91 586 1.32e-29 125
MS.gene20274.t1 AT4G19990 24.634 410 264 7 219 625 106 473 2.76e-28 120
MS.gene20274.t1 AT5G28530 22.266 512 302 17 40 540 60 486 4.56e-18 88.6
MS.gene20274.t1 AT5G28530 22.070 512 299 18 40 540 60 482 2.21e-17 86.3

Find 93 sgRNAs with CRISPR-Local

Find 133 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTCGATGAGTTAACTAATT+TGG 0.156761 5.1:+8366875 MS.gene20274:CDS
CTTTAGTCATTTCAACATTA+TGG 0.194287 5.1:+8367142 MS.gene20274:CDS
TACATTTCAGCTGCCTTTCT+TGG 0.197151 5.1:+8366594 MS.gene20274:intron
CAACAAGATTATGCTCTATT+TGG 0.198611 5.1:+8366071 MS.gene20274:CDS
CATCGTAAGAGTGTGTTATT+TGG 0.276792 5.1:+8366627 MS.gene20274:CDS
TATGGTTTGAGGTTTCTTAT+TGG 0.280533 5.1:-8366721 None:intergenic
GGTTTCAAATGTTGATGTTT+TGG 0.317051 5.1:+8367436 MS.gene20274:CDS
AATCAGATTTCGACTACAAT+TGG 0.318540 5.1:+8366916 MS.gene20274:CDS
ACCAGCCTGGTCTGTGTATA+TGG 0.341000 5.1:-8366739 None:intergenic
GCCGATATTTATTGTTTGTT+CGG 0.341758 5.1:-8366121 None:intergenic
GAGGGATAATCATGCTGATT+TGG 0.346844 5.1:+8367175 MS.gene20274:CDS
TATGCATTGGTCTTCAGTTT+GGG 0.370760 5.1:+8367016 MS.gene20274:CDS
AACAATAAATATCGGCCATT+AGG 0.388497 5.1:+8366128 MS.gene20274:CDS
TCATGCTGATTTGGACTTCA+AGG 0.389304 5.1:+8367184 MS.gene20274:CDS
ACCGAACAAACAATAAATAT+CGG 0.389395 5.1:+8366120 MS.gene20274:CDS
GTATGCATTGGTCTTCAGTT+TGG 0.392120 5.1:+8367015 MS.gene20274:CDS
TTCTCATACTCAACAAGAAC+TGG 0.395633 5.1:-8365591 None:intergenic
CCATGATTCTTTATCACTAA+AGG 0.406174 5.1:-8365352 None:intergenic
TAAAGAATCATGGAAGTCTT+TGG 0.408500 5.1:+8365362 MS.gene20274:CDS
CATGTCGTATGTTTGAGAAC+CGG 0.411671 5.1:+8367561 MS.gene20274:CDS
GATTATGATCTTCAAACCTT+GGG 0.413114 5.1:-8367484 None:intergenic
ATAACACTGGATTCTCTGTT+AGG 0.417808 5.1:+8365433 MS.gene20274:CDS
GGGTTGTCACAGTCGACATT+AGG 0.419786 5.1:+8367519 MS.gene20274:CDS
CGATTATGATCTTCAAACCT+TGG 0.425681 5.1:-8367485 None:intergenic
CGAGAAGTAAATGAAGTTCA+AGG 0.430014 5.1:+8365768 MS.gene20274:CDS
AGCTGGAATATATTTGAAGT+TGG 0.433136 5.1:+8365650 MS.gene20274:CDS
TGAATGTCTATAAGATATAT+AGG 0.445219 5.1:+8365682 MS.gene20274:CDS
TGGTCTGTGTATATGGTTTG+AGG 0.447350 5.1:-8366732 None:intergenic
GAAAGGGTTGGTTGTAAAGC+TGG 0.447509 5.1:+8365633 MS.gene20274:CDS
CGTTATGCTTGTAACAAAGA+AGG 0.449439 5.1:+8365516 MS.gene20274:CDS
GCTTTAATAAATTCTAAAGC+TGG 0.450264 5.1:+8365798 MS.gene20274:CDS
ATCTGAGAGTGGACAATTGA+CGG 0.452529 5.1:+8365287 MS.gene20274:CDS
TGGTCTTTGCTCATGGCACA+TGG 0.452802 5.1:+8366806 MS.gene20274:CDS
CTCCTCAAAATAGTATTTGA+AGG 0.453936 5.1:-8367318 None:intergenic
ATGTCGTATGTTTGAGAACC+GGG 0.461344 5.1:+8367562 MS.gene20274:CDS
GGCCTACTTCAGACTTTAAG+TGG 0.462107 5.1:+8366970 MS.gene20274:CDS
ACAAAATAAGAAATCGGATA+AGG 0.469178 5.1:+8365482 MS.gene20274:CDS
TTTGAGGAGGACACAACAAA+AGG 0.469432 5.1:+8367332 MS.gene20274:CDS
TTACAACCAACCCTTTCCTC+TGG 0.485330 5.1:-8365627 None:intergenic
TTTAATTATGCATATAACAC+TGG 0.485542 5.1:+8365420 MS.gene20274:CDS
TCTAATGACGACTCGTCAAA+AGG 0.488508 5.1:+8365911 MS.gene20274:CDS
CAATTCACAAAATAAGAAAT+CGG 0.490263 5.1:+8365476 MS.gene20274:CDS
ATGATTGTCAAAACCAAGAA+AGG 0.491378 5.1:-8366607 None:intergenic
ATCAGATTTCGACTACAATT+GGG 0.491708 5.1:+8366917 MS.gene20274:CDS
CGTCAAAAGGAGATGGTGGC+TGG 0.492100 5.1:+8365924 MS.gene20274:CDS
ACATTATGGTTCAGAGAATG+AGG 0.494022 5.1:+8367156 MS.gene20274:CDS
GACCGCATAAGCTGACTGTT+GGG 0.496307 5.1:-8367236 None:intergenic
TGTCTTCTGCAGCATCAACT+TGG 0.502977 5.1:-8366016 None:intergenic
CTCATTCAAATCGTATGCAT+TGG 0.507227 5.1:+8367003 MS.gene20274:CDS
ATCATAATCGACTTGATGAA+AGG 0.507955 5.1:+8367498 MS.gene20274:CDS
TTTAAGTCCGAAAATCGAAA+AGG 0.508080 5.1:-8365882 None:intergenic
ATTGGTCTTCAGTTTGGGTG+AGG 0.509410 5.1:+8367021 MS.gene20274:CDS
GATCTTCAAACCTTGGGGAA+AGG 0.519004 5.1:-8367478 None:intergenic
CAAAATAGTATTTGAAGGAA+AGG 0.519540 5.1:-8367313 None:intergenic
TAAAGAATCGAACAAGTGAA+TGG 0.519618 5.1:-8367124 None:intergenic
AAATGCTCTGCTTTATGTAA+TGG 0.525118 5.1:-8365726 None:intergenic
TCATAATCGACTTGATGAAA+GGG 0.526860 5.1:+8367499 MS.gene20274:CDS
TTGAGGAGGACACAACAAAA+GGG 0.529908 5.1:+8367333 MS.gene20274:CDS
TGTCTATAAGATATATAGGT+GGG 0.532209 5.1:+8365686 MS.gene20274:CDS
TAACAAAGAAGGTTTCAAGA+AGG 0.538422 5.1:+8365527 MS.gene20274:CDS
AATCGAACAAGTGAATGGTC+TGG 0.540971 5.1:-8367119 None:intergenic
CCTTTAGTGATAAAGAATCA+TGG 0.543522 5.1:+8365352 MS.gene20274:CDS
TATATCTTATAGACATTCAA+CGG 0.544310 5.1:-8365678 None:intergenic
AACAAAGAAGGTTTCAAGAA+GGG 0.545537 5.1:+8365528 MS.gene20274:CDS
CGATGTTGATTATAATGAGA+GGG 0.549585 5.1:+8367415 MS.gene20274:CDS
GAGAACATCTTATGAAGTGA+TGG 0.551307 5.1:+8365830 MS.gene20274:CDS
ACTTCAACAACGCACCAGCC+TGG 0.557139 5.1:-8366752 None:intergenic
AATGAAGCTCTCTCGAAACC+AGG 0.565261 5.1:+8365978 MS.gene20274:CDS
GGGTGAAGGTGTTGGAGGCA+CGG 0.565649 5.1:+8365851 MS.gene20274:CDS
TGTAAATTCAGTTATTGATG+CGG 0.568873 5.1:+8365311 MS.gene20274:CDS
GGACCGCATAAGCTGACTGT+TGG 0.579264 5.1:-8367237 None:intergenic
TGGTGTATACTTGTTTACTA+CGG 0.585960 5.1:-8367258 None:intergenic
ACCATATACACAGACCAGGC+TGG 0.590176 5.1:+8366738 MS.gene20274:CDS
TTGAAGGAAACCTTTCCCCA+AGG 0.600566 5.1:+8367468 MS.gene20274:CDS
GAAGTGATGGGTGAAGGTGT+TGG 0.603704 5.1:+8365843 MS.gene20274:CDS
GACGACTCGTCAAAAGGAGA+TGG 0.604288 5.1:+8365917 MS.gene20274:CDS
ATCCCAACAGTCAGCTTATG+CGG 0.606153 5.1:+8367234 MS.gene20274:CDS
GACTCGTCAAAAGGAGATGG+TGG 0.611157 5.1:+8365920 MS.gene20274:CDS
ATATCAACTTCATCTGAGAG+TGG 0.614980 5.1:+8365276 MS.gene20274:CDS
AGCCACTTAAAGTCTGAAGT+AGG 0.617789 5.1:-8366972 None:intergenic
AGAACTGGACTGTCACATGT+TGG 0.620791 5.1:-8365576 None:intergenic
GTGATGGGTGAAGGTGTTGG+AGG 0.621481 5.1:+8365846 MS.gene20274:CDS
ACGATGTTGATTATAATGAG+AGG 0.622271 5.1:+8367414 MS.gene20274:CDS
ATTATGATCTTCAAACCTTG+GGG 0.630358 5.1:-8367483 None:intergenic
GGTCTTTGCTCATGGCACAT+GGG 0.639768 5.1:+8366807 MS.gene20274:CDS
AGAACATCTTATGAAGTGAT+GGG 0.643044 5.1:+8365831 MS.gene20274:CDS
CATTATGGTTCAGAGAATGA+GGG 0.643278 5.1:+8367157 MS.gene20274:CDS
ATGTCTATAAGATATATAGG+TGG 0.643489 5.1:+8365685 MS.gene20274:CDS
CATGTCAACATAAGAAACCC+CGG 0.650024 5.1:-8367580 None:intergenic
TGAATAAATGAAAAGCAAGA+TGG 0.655697 5.1:-8367278 None:intergenic
TCTTATGAAGTGATGGGTGA+AGG 0.656961 5.1:+8365837 MS.gene20274:CDS
TCAAACCATATACACAGACC+AGG 0.671057 5.1:+8366734 MS.gene20274:CDS
TGTCGTATGTTTGAGAACCG+GGG 0.673760 5.1:+8367563 MS.gene20274:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATGATGAAAAATTGTTTTAA+TGG + chr5.1:8366945-8366964 MS.gene20274:CDS 15.0%
!! CAATTCACAAAATAAGAAAT+CGG + chr5.1:8365476-8365495 MS.gene20274:CDS 20.0%
!! TATATCTTATAGACATTCAA+CGG - chr5.1:8365681-8365700 None:intergenic 20.0%
!! TGAATGTCTATAAGATATAT+AGG + chr5.1:8365682-8365701 MS.gene20274:CDS 20.0%
!! TTTAATTATGCATATAACAC+TGG + chr5.1:8365420-8365439 MS.gene20274:CDS 20.0%
!!! AATTTAATGTTCTGGTTTAT+TGG + chr5.1:8366303-8366322 MS.gene20274:intron 20.0%
!!! TGAAAAATTGTTTTAATGGA+AGG + chr5.1:8366949-8366968 MS.gene20274:CDS 20.0%
!!! TGAATGTTTATATTGATTGA+AGG + chr5.1:8366275-8366294 MS.gene20274:intron 20.0%
! AAAACACCTTGAAAAACATT+CGG - chr5.1:8366783-8366802 None:intergenic 25.0%
! ACAAAATAAGAAATCGGATA+AGG + chr5.1:8365482-8365501 MS.gene20274:CDS 25.0%
! ACCGAACAAACAATAAATAT+CGG + chr5.1:8366120-8366139 MS.gene20274:CDS 25.0%
! AGTACAAAATACTAACCTAA+TGG - chr5.1:8366146-8366165 None:intergenic 25.0%
! ATGTCTATAAGATATATAGG+TGG + chr5.1:8365685-8365704 MS.gene20274:CDS 25.0%
! CAAAATAGTATTTGAAGGAA+AGG - chr5.1:8367316-8367335 None:intergenic 25.0%
! CTTTAGTCATTTCAACATTA+TGG + chr5.1:8367142-8367161 MS.gene20274:CDS 25.0%
! TGAAATTTGAATGAATGTTC+TGG + chr5.1:8366193-8366212 MS.gene20274:intron 25.0%
! TGAATAAATGAAAAGCAAGA+TGG - chr5.1:8367281-8367300 None:intergenic 25.0%
! TGTAAATTCAGTTATTGATG+CGG + chr5.1:8365311-8365330 MS.gene20274:CDS 25.0%
! TGTCTATAAGATATATAGGT+GGG + chr5.1:8365686-8365705 MS.gene20274:CDS 25.0%
! TTTCGATGAGTTAACTAATT+TGG + chr5.1:8366875-8366894 MS.gene20274:CDS 25.0%
!! AGGTTTAGAATTTAATGTTC+TGG + chr5.1:8366295-8366314 MS.gene20274:intron 25.0%
!! CAAAGTTTTTACAATTGAGA+AGG + chr5.1:8367376-8367395 MS.gene20274:CDS 25.0%
!! TGTTGTATTTTGTTCTGATT+TGG + chr5.1:8366565-8366584 MS.gene20274:intron 25.0%
!! TTCCTTCAAATACTATTTTG+AGG + chr5.1:8367316-8367335 MS.gene20274:CDS 25.0%
!!! AAGACATAGCTAGTATTTTT+TGG + chr5.1:8366033-8366052 MS.gene20274:CDS 25.0%
!!! AGACATAGCTAGTATTTTTT+GGG + chr5.1:8366034-8366053 MS.gene20274:CDS 25.0%
!!! CTTATTTTGTGAATTGTTCT+TGG - chr5.1:8365472-8365491 None:intergenic 25.0%
!!! GCTTTAATAAATTCTAAAGC+TGG + chr5.1:8365798-8365817 MS.gene20274:CDS 25.0%
AAACACATATGATCTTTCCA+TGG - chr5.1:8366366-8366385 None:intergenic 30.0%
AAATGCTCTGCTTTATGTAA+TGG - chr5.1:8365729-8365748 None:intergenic 30.0%
AACAAAGAAGGTTTCAAGAA+GGG + chr5.1:8365528-8365547 MS.gene20274:CDS 30.0%
AACAATAAATATCGGCCATT+AGG + chr5.1:8366128-8366147 MS.gene20274:CDS 30.0%
AATCAGATTTCGACTACAAT+TGG + chr5.1:8366916-8366935 MS.gene20274:CDS 30.0%
AGAACATCTTATGAAGTGAT+GGG + chr5.1:8365831-8365850 MS.gene20274:CDS 30.0%
ATCAGATTTCGACTACAATT+GGG + chr5.1:8366917-8366936 MS.gene20274:CDS 30.0%
ATGATTGTCAAAACCAAGAA+AGG - chr5.1:8366610-8366629 None:intergenic 30.0%
ATTATGATCTTCAAACCTTG+GGG - chr5.1:8367486-8367505 None:intergenic 30.0%
CAACAAGATTATGCTCTATT+TGG + chr5.1:8366071-8366090 MS.gene20274:CDS 30.0%
CACAAATTCTAAGTCAAGAT+AGG - chr5.1:8366333-8366352 None:intergenic 30.0%
CCATGATTCTTTATCACTAA+AGG - chr5.1:8365355-8365374 None:intergenic 30.0%
CCTTTAGTGATAAAGAATCA+TGG + chr5.1:8365352-8365371 MS.gene20274:CDS 30.0%
CTCCTCAAAATAGTATTTGA+AGG - chr5.1:8367321-8367340 None:intergenic 30.0%
CTGGTATATGTGTGTTAATT+TGG + chr5.1:8366232-8366251 MS.gene20274:intron 30.0%
GAGAATGCAAAGAAAAATCT+CGG + chr5.1:8366831-8366850 MS.gene20274:CDS 30.0%
GATTATGATCTTCAAACCTT+GGG - chr5.1:8367487-8367506 None:intergenic 30.0%
GCCGATATTTATTGTTTGTT+CGG - chr5.1:8366124-8366143 None:intergenic 30.0%
TAAAGAAAAACCAGAGGAAA+GGG + chr5.1:8365617-8365636 MS.gene20274:CDS 30.0%
TAAAGAATCATGGAAGTCTT+TGG + chr5.1:8365362-8365381 MS.gene20274:CDS 30.0%
TAAAGAATCGAACAAGTGAA+TGG - chr5.1:8367127-8367146 None:intergenic 30.0%
TAACAAAGAAGGTTTCAAGA+AGG + chr5.1:8365527-8365546 MS.gene20274:CDS 30.0%
TGGATATCATAGTAAAAACC+TGG - chr5.1:8365999-8366018 None:intergenic 30.0%
TGGTGTATACTTGTTTACTA+CGG - chr5.1:8367261-8367280 None:intergenic 30.0%
TTTAAGTCCGAAAATCGAAA+AGG - chr5.1:8365885-8365904 None:intergenic 30.0%
TTTCTATTTACACAGCTCAA+TGG + chr5.1:8366524-8366543 MS.gene20274:intron 30.0%
! ACGATGTTGATTATAATGAG+AGG + chr5.1:8367414-8367433 MS.gene20274:CDS 30.0%
! AGCTGGAATATATTTGAAGT+TGG + chr5.1:8365650-8365669 MS.gene20274:CDS 30.0%
! CGATGTTGATTATAATGAGA+GGG + chr5.1:8367415-8367434 MS.gene20274:CDS 30.0%
! GAATCTTCCTTTTCGATTTT+CGG + chr5.1:8365875-8365894 MS.gene20274:CDS 30.0%
! GGTTTCAAATGTTGATGTTT+TGG + chr5.1:8367436-8367455 MS.gene20274:CDS 30.0%
! TATGGTTTGAGGTTTCTTAT+TGG - chr5.1:8366724-8366743 None:intergenic 30.0%
! TCAATTCCGAATGTTTTTCA+AGG + chr5.1:8366774-8366793 MS.gene20274:CDS 30.0%
!! ATCATAATCGACTTGATGAA+AGG + chr5.1:8367498-8367517 MS.gene20274:CDS 30.0%
!! ATGTTCTGGTTTAGAATTGA+AGG + chr5.1:8366207-8366226 MS.gene20274:intron 30.0%
!! CTTCAAATACTATTTTGAGG+AGG + chr5.1:8367319-8367338 MS.gene20274:CDS 30.0%
!! TCATAATCGACTTGATGAAA+GGG + chr5.1:8367499-8367518 MS.gene20274:CDS 30.0%
!!! GTTTTTCAAGGTGTTTTTCA+TGG + chr5.1:8366786-8366805 MS.gene20274:CDS 30.0%
ACATTATGGTTCAGAGAATG+AGG + chr5.1:8367156-8367175 MS.gene20274:CDS 35.0%
ATAACACTGGATTCTCTGTT+AGG + chr5.1:8365433-8365452 MS.gene20274:CDS 35.0%
ATATCAACTTCATCTGAGAG+TGG + chr5.1:8365276-8365295 MS.gene20274:CDS 35.0%
CATTATGGTTCAGAGAATGA+GGG + chr5.1:8367157-8367176 MS.gene20274:CDS 35.0%
CGAGAAGTAAATGAAGTTCA+AGG + chr5.1:8365768-8365787 MS.gene20274:CDS 35.0%
CGATTATGATCTTCAAACCT+TGG - chr5.1:8367488-8367507 None:intergenic 35.0%
CGTTATGCTTGTAACAAAGA+AGG + chr5.1:8365516-8365535 MS.gene20274:CDS 35.0%
CTAAAGAAAAACCAGAGGAA+AGG + chr5.1:8365616-8365635 MS.gene20274:CDS 35.0%
CTCATTCAAATCGTATGCAT+TGG + chr5.1:8367003-8367022 MS.gene20274:CDS 35.0%
GAAAGCTAAAGAAAAACCAG+AGG + chr5.1:8365611-8365630 MS.gene20274:CDS 35.0%
GAGAACATCTTATGAAGTGA+TGG + chr5.1:8365830-8365849 MS.gene20274:CDS 35.0%
GTCTGCTTAATCCTAAAAAC+AGG + chr5.1:8365553-8365572 MS.gene20274:CDS 35.0%
TTCTCATACTCAACAAGAAC+TGG - chr5.1:8365594-8365613 None:intergenic 35.0%
! CATCGTAAGAGTGTGTTATT+TGG + chr5.1:8366627-8366646 MS.gene20274:CDS 35.0%
! TCTTTCAATGCTTTTCTTCG+TGG + chr5.1:8367086-8367105 MS.gene20274:CDS 35.0%
! TTTTTCATGGTCTTTGCTCA+TGG + chr5.1:8366799-8366818 MS.gene20274:CDS 35.0%
!! TATGCATTGGTCTTCAGTTT+GGG + chr5.1:8367016-8367035 MS.gene20274:CDS 35.0%
!!! AGACATCAGCTACTTTTGAT+TGG + chr5.1:8366670-8366689 MS.gene20274:CDS 35.0%
AATCGAACAAGTGAATGGTC+TGG - chr5.1:8367122-8367141 None:intergenic 40.0%
ACCTATGATCTTTGCATAGC+TGG - chr5.1:8366464-8366483 None:intergenic 40.0%
ATCTGAGAGTGGACAATTGA+CGG + chr5.1:8365287-8365306 MS.gene20274:CDS 40.0%
ATGTCGTATGTTTGAGAACC+GGG + chr5.1:8367562-8367581 MS.gene20274:CDS 40.0%
CATGTCGTATGTTTGAGAAC+CGG + chr5.1:8367561-8367580 MS.gene20274:CDS 40.0%
TACATTTCAGCTGCCTTTCT+TGG + chr5.1:8366594-8366613 MS.gene20274:intron 40.0%
TCAAACCATATACACAGACC+AGG + chr5.1:8366734-8366753 MS.gene20274:CDS 40.0%
TCCAGCTATGCAAAGATCAT+AGG + chr5.1:8366460-8366479 MS.gene20274:intron 40.0%
TCTAATGACGACTCGTCAAA+AGG + chr5.1:8365911-8365930 MS.gene20274:CDS 40.0%
TGGTCTGTGTATATGGTTTG+AGG - chr5.1:8366735-8366754 None:intergenic 40.0%
TTGAGGAGGACACAACAAAA+GGG + chr5.1:8367333-8367352 MS.gene20274:CDS 40.0%
TTTGAGGAGGACACAACAAA+AGG + chr5.1:8367332-8367351 MS.gene20274:CDS 40.0%
! AGCCACTTAAAGTCTGAAGT+AGG - chr5.1:8366975-8366994 None:intergenic 40.0%
! GAGGGATAATCATGCTGATT+TGG + chr5.1:8367175-8367194 MS.gene20274:CDS 40.0%
! TGGTTTAGAATTGAAGGCTC+TGG + chr5.1:8366213-8366232 MS.gene20274:intron 40.0%
!! GTATGCATTGGTCTTCAGTT+TGG + chr5.1:8367015-8367034 MS.gene20274:CDS 40.0%
!! GTTTTGGATGCAGAACTTGA+AGG + chr5.1:8367452-8367471 MS.gene20274:CDS 40.0%
!! TCACATGTTGGCCTGTTTTT+AGG - chr5.1:8365567-8365586 None:intergenic 40.0%
!! TCATGCTGATTTGGACTTCA+AGG + chr5.1:8367184-8367203 MS.gene20274:CDS 40.0%
!! TCTTATGAAGTGATGGGTGA+AGG + chr5.1:8365837-8365856 MS.gene20274:CDS 40.0%
!!! GCTAGTATTTTTTGGGCAGA+TGG + chr5.1:8366041-8366060 MS.gene20274:CDS 40.0%
AATGAAGCTCTCTCGAAACC+AGG + chr5.1:8365978-8365997 MS.gene20274:CDS 45.0%
AGAACTGGACTGTCACATGT+TGG - chr5.1:8365579-8365598 None:intergenic 45.0%
ATCCCAACAGTCAGCTTATG+CGG + chr5.1:8367234-8367253 MS.gene20274:CDS 45.0%
ATTTGTGCTCTGTACAGCCA+TGG + chr5.1:8366346-8366365 MS.gene20274:intron 45.0%
GAAAAACCAGAGGAAAGGGT+TGG + chr5.1:8365621-8365640 MS.gene20274:CDS 45.0%
GATCTTCAAACCTTGGGGAA+AGG - chr5.1:8367481-8367500 None:intergenic 45.0%
GGCCTACTTCAGACTTTAAG+TGG + chr5.1:8366970-8366989 MS.gene20274:CDS 45.0%
TGTCGTATGTTTGAGAACCG+GGG + chr5.1:8367563-8367582 MS.gene20274:CDS 45.0%
TGTCTTCTGCAGCATCAACT+TGG - chr5.1:8366019-8366038 None:intergenic 45.0%
TTACAACCAACCCTTTCCTC+TGG - chr5.1:8365630-8365649 None:intergenic 45.0%
TTGAAGGAAACCTTTCCCCA+AGG + chr5.1:8367468-8367487 MS.gene20274:CDS 45.0%
! GTGAGGTCACATTTTACAGC+TGG + chr5.1:8367038-8367057 MS.gene20274:CDS 45.0%
!! ATTGGTCTTCAGTTTGGGTG+AGG + chr5.1:8367021-8367040 MS.gene20274:CDS 45.0%
!! GAAAGGGTTGGTTGTAAAGC+TGG + chr5.1:8365633-8365652 MS.gene20274:CDS 45.0%
ACCAGCCTGGTCTGTGTATA+TGG - chr5.1:8366742-8366761 None:intergenic 50.0%
ACCATATACACAGACCAGGC+TGG + chr5.1:8366738-8366757 MS.gene20274:CDS 50.0%
GACCGCATAAGCTGACTGTT+GGG - chr5.1:8367239-8367258 None:intergenic 50.0%
GACGACTCGTCAAAAGGAGA+TGG + chr5.1:8365917-8365936 MS.gene20274:CDS 50.0%
GACTCGTCAAAAGGAGATGG+TGG + chr5.1:8365920-8365939 MS.gene20274:CDS 50.0%
GGGTTGTCACAGTCGACATT+AGG + chr5.1:8367519-8367538 MS.gene20274:CDS 50.0%
GGTCTTTGCTCATGGCACAT+GGG + chr5.1:8366807-8366826 MS.gene20274:CDS 50.0%
TGGTCTTTGCTCATGGCACA+TGG + chr5.1:8366806-8366825 MS.gene20274:CDS 50.0%
!! GAAGTGATGGGTGAAGGTGT+TGG + chr5.1:8365843-8365862 MS.gene20274:CDS 50.0%
ACTTCAACAACGCACCAGCC+TGG - chr5.1:8366755-8366774 None:intergenic 55.0%
GGACCGCATAAGCTGACTGT+TGG - chr5.1:8367240-8367259 None:intergenic 55.0%
! CGTCAAAAGGAGATGGTGGC+TGG + chr5.1:8365924-8365943 MS.gene20274:CDS 55.0%
!! GTGATGGGTGAAGGTGTTGG+AGG + chr5.1:8365846-8365865 MS.gene20274:CDS 55.0%
!! GGGTGAAGGTGTTGGAGGCA+CGG + chr5.1:8365851-8365870 MS.gene20274:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 8365267 8367598 8365267 ID=MS.gene20274
chr5.1 mRNA 8365267 8367598 8365267 ID=MS.gene20274.t1;Parent=MS.gene20274
chr5.1 exon 8365267 8366149 8365267 ID=MS.gene20274.t1.exon1;Parent=MS.gene20274.t1
chr5.1 CDS 8365267 8366149 8365267 ID=cds.MS.gene20274.t1;Parent=MS.gene20274.t1
chr5.1 exon 8366604 8367598 8366604 ID=MS.gene20274.t1.exon2;Parent=MS.gene20274.t1
chr5.1 CDS 8366604 8367598 8366604 ID=cds.MS.gene20274.t1;Parent=MS.gene20274.t1
Gene Sequence

>MS.gene20274

ATGGATTTGATATCAACTTCATCTGAGAGTGGACAATTGACGGTTGTAAATTCAGTTATTGATGCGGATAGTTCTCAATGTTACTCCTTTAGTGATAAAGAATCATGGAAGTCTTTGGTCTTTAAATCTATAAAAGAGATTGAAGATTTTTATTTTAATTATGCATATAACACTGGATTCTCTGTTAGGAGTATGTTGAAGTCCAAGAACAATTCACAAAATAAGAAATCGGATAAGGTACATTATGTGCGTTATGCTTGTAACAAAGAAGGTTTCAAGAAGGGAAGTCTGCTTAATCCTAAAAACAGGCCAACATGTGACAGTCCAGTTCTTGTTGAGTATGAGAAAGCTAAAGAAAAACCAGAGGAAAGGGTTGGTTGTAAAGCTGGAATATATTTGAAGTTGGATGAACCGTTGAATGTCTATAAGATATATAGGTGGGTAGTTGATCACTGTCATCCATTACATAAAGCAGAGCATTTGCGCTACTTGAGATCATTTCGAGAAGTAAATGAAGTTCAAGGACAACTTGCTTTAATAAATTCTAAAGCTGGAATGTCGATGAGAACATCTTATGAAGTGATGGGTGAAGGTGTTGGAGGCACGGAGAATCTTCCTTTTCGATTTTCGGACTTAAAGAACTATCTAATGACGACTCGTCAAAAGGAGATGGTGGCTGGTGAAGCAACTGTTATTCAAGAATTCTTTAGAAATGAAGCTCTCTCGAAACCAGGTTTTTACTATGATATCCAAGTTGATGCTGCAGAAGACATAGCTAGTATTTTTTGGGCAGATGGAATTATGCAACAAGATTATGCTCTATTTGGTGATGTTGTCAGTTTTGATACAACTTACCGAACAAACAATAAATATCGGCCATTAGGTTAGTATTTTGTACTTCTCACAGTTTTTAATTCTTATATTCTTGAAATTTGAATGAATGTTCTGGTTTAGAATTGAAGGCTCTGGTATATGTGTGTTAATTTGGCTCTTGTGTGTGTTTAGAATTGAATGTTTATATTGATTGAAGGTTTAGAATTTAATGTTCTGGTTTATTGGTGATCCTATCTTGACTTAGAATTTGTGCTCTGTACAGCCATGGAAAGATCATATGTGTTTTTTTATCGATGATAAATAAATAGCTCTTTCTGTGTTTGCGAGTTTAGACATCTCGCTTGAGAAGTTGTGCTATGTCCAGCTATGCAAAGATCATAGGTGTTTTTTTGTGTTTTTTTACAGTTGTTAAATGAATAGCTTTTTCTATTTACACAGCTCAATGGTTTTATATTTTTTAAATATGTTGTATTTTGTTCTGATTTGGTTCATTTACATTTCAGCTGCCTTTCTTGGTTTTGACAATCATCGTAAGAGTGTGTTATTTGGTGCTGCGCTACTGTATGATGAGACATCAGCTACTTTTGATTGGTTGTTTACAACTTTTTTGAAGTGTATGTCCAATAAGAAACCTCAAACCATATACACAGACCAGGCTGGTGCGTTGTTGAAGTCAATTCCGAATGTTTTTCAAGGTGTTTTTCATGGTCTTTGCTCATGGCACATGGGAGAGAATGCAAAGAAAAATCTCGGCTCTCGTGCAAACAGTGCATTTTTCGATGAGTTAACTAATTTGGTTTCAAATGTCGAAGATGAATCAGATTTCGACTACAATTGGGATCAGATGATGAAAAATTGTTTTAATGGAAGGCCTACTTCAGACTTTAAGTGGCTTGTTCAAACTCATTCAAATCGTATGCATTGGTCTTCAGTTTGGGTGAGGTCACATTTTACAGCTGGTTTGAAAACGACTCAGTTAAGTGAGTCTTTCAATGCTTTTCTTCGTGGATTTTTGCAGCCAGACCATTCACTTGTTCGATTCTTTAGTCATTTCAACATTATGGTTCAGAGAATGAGGGATAATCATGCTGATTTGGACTTCAAGGCTGCAAACACTAGAACAAAAAATAATTATCCCAACAGTCAGCTTATGCGGTCCGTAGTAAACAAGTATACACCATCTTGCTTTTCATTTATTCACAGTCAGTATGACCTTTCCTTCAAATACTATTTTGAGGAGGACACAACAAAAGGGTCTGAATTGAACAAGTTCTTCAAAGTTTTTACAATTGAGAAGGTTGATTATAATTACGACGATGTTGATTATAATGAGAGGGTTTCAAATGTTGATGTTTTGGATGCAGAACTTGAAGGAAACCTTTCCCCAAGGTTTGAAGATCATAATCGACTTGATGAAAGGGTTGTCACAGTCGACATTAGGAGCAAAAGTTTTACTTGTTCATGTCGTATGTTTGAGAACCGGGGTTTCTTATGTTGA
Protein sequence

>MS.gene20274.t1

MDLISTSSESGQLTVVNSVIDADSSQCYSFSDKESWKSLVFKSIKEIEDFYFNYAYNTGFSVRSMLKSKNNSQNKKSDKVHYVRYACNKEGFKKGSLLNPKNRPTCDSPVLVEYEKAKEKPEERVGCKAGIYLKLDEPLNVYKIYRWVVDHCHPLHKAEHLRYLRSFREVNEVQGQLALINSKAGMSMRTSYEVMGEGVGGTENLPFRFSDLKNYLMTTRQKEMVAGEATVIQEFFRNEALSKPGFYYDIQVDAAEDIASIFWADGIMQQDYALFGDVVSFDTTYRTNNKYRPLAAFLGFDNHRKSVLFGAALLYDETSATFDWLFTTFLKCMSNKKPQTIYTDQAGALLKSIPNVFQGVFHGLCSWHMGENAKKNLGSRANSAFFDELTNLVSNVEDESDFDYNWDQMMKNCFNGRPTSDFKWLVQTHSNRMHWSSVWVRSHFTAGLKTTQLSESFNAFLRGFLQPDHSLVRFFSHFNIMVQRMRDNHADLDFKAANTRTKNNYPNSQLMRSVVNKYTPSCFSFIHSQYDLSFKYYFEEDTTKGSELNKFFKVFTIEKVDYNYDDVDYNERVSNVDVLDAELEGNLSPRFEDHNRLDERVVTVDIRSKSFTCSCRMFENRGFLC