AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20277

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20277.t1 MTR_5g015950 94.005 417 25 0 1 417 32 448 0.0 803
MS.gene20277.t1 MTR_3g053270 94.005 417 25 0 1 417 32 448 0.0 803
MS.gene20277.t1 MTR_4g095500 69.249 426 111 6 1 417 56 470 0.0 579
MS.gene20277.t1 MTR_2g089100 44.755 429 193 8 1 417 49 445 1.17e-121 361
MS.gene20277.t1 MTR_4g097080 27.553 421 252 9 2 416 131 504 5.91e-49 174
MS.gene20277.t1 MTR_5g015490 27.446 419 257 11 4 416 114 491 2.85e-44 160
MS.gene20277.t1 MTR_1g029420 26.747 415 256 6 2 414 223 591 7.99e-41 153
MS.gene20277.t1 MTR_3g089055 27.251 422 245 8 2 414 196 564 9.36e-41 152
MS.gene20277.t1 MTR_3g089055 27.251 422 245 8 2 414 201 569 1.25e-40 152
MS.gene20277.t1 MTR_2g099110 26.959 434 243 13 3 416 73 452 6.37e-37 140
MS.gene20277.t1 MTR_2g082090 26.478 423 249 10 1 414 209 578 6.19e-35 136
MS.gene20277.t1 MTR_3g056110 24.286 420 262 9 1 414 172 541 3.49e-34 134
MS.gene20277.t1 MTR_5g094450 24.356 427 257 10 1 414 158 531 3.75e-34 134
MS.gene20277.t1 MTR_5g097480 25.708 424 251 10 1 414 174 543 3.00e-33 131
MS.gene20277.t1 MTR_4g133660 25.581 430 247 11 1 415 172 543 2.08e-32 129
MS.gene20277.t1 MTR_8g442410 26.316 418 251 13 5 414 156 524 2.41e-32 128
MS.gene20277.t1 MTR_4g064150 24.350 423 269 12 1 417 358 735 3.67e-31 126
MS.gene20277.t1 MTR_8g020840 26.651 424 255 12 2 414 169 547 9.03e-31 124
MS.gene20277.t1 MTR_2g026250 26.178 382 226 9 39 414 264 595 1.72e-29 120
MS.gene20277.t1 MTR_7g027190 26.777 422 249 14 4 414 300 672 4.32e-29 120
MS.gene20277.t1 MTR_2g097410 24.408 422 268 13 1 417 368 743 7.57e-29 119
MS.gene20277.t1 MTR_3g065980 25.467 428 240 10 5 414 173 539 1.23e-28 118
MS.gene20277.t1 MTR_4g064180 23.697 422 268 15 1 415 251 625 6.04e-28 116
MS.gene20277.t1 MTR_4g104020 27.123 424 245 14 3 414 148 519 1.69e-27 114
MS.gene20277.t1 MTR_4g064120 23.641 423 267 15 1 415 251 625 2.96e-27 114
MS.gene20277.t1 MTR_3g022830 24.658 438 274 12 5 414 26 435 3.43e-25 107
MS.gene20277.t1 MTR_4g064160 24.823 423 263 15 1 417 313 686 1.11e-24 107
MS.gene20277.t1 MTR_4g102790 23.571 420 266 12 4 416 292 663 1.46e-24 106
MS.gene20277.t1 MTR_4g064200 24.582 419 261 15 4 417 284 652 2.20e-24 105
MS.gene20277.t1 MTR_2g097310 23.404 423 267 13 1 417 269 640 1.33e-22 100
MS.gene20277.t1 MTR_7g062120 22.406 424 272 11 1 417 369 742 1.80e-22 100
MS.gene20277.t1 MTR_5g009080 24.256 437 290 11 4 414 56 477 8.40e-22 97.8
MS.gene20277.t1 MTR_4g076140 23.210 461 264 13 4 414 48 468 8.70e-22 97.4
MS.gene20277.t1 MTR_2g097473 21.262 428 263 11 4 417 289 656 1.96e-21 97.1
MS.gene20277.t1 MTR_7g074650 22.573 412 258 12 7 416 441 793 3.38e-21 96.7
MS.gene20277.t1 MTR_7g057230 26.875 320 190 7 101 414 375 656 3.51e-21 96.3
MS.gene20277.t1 MTR_2g097463 22.488 418 270 11 4 417 290 657 4.10e-21 96.3
MS.gene20277.t1 MTR_2g097467 21.343 417 276 11 4 417 289 656 6.82e-21 95.5
MS.gene20277.t1 MTR_4g077760 26.024 415 248 12 5 414 194 554 1.21e-20 94.4
MS.gene20277.t1 MTR_1g069725 22.169 415 258 12 4 414 109 462 3.98e-19 89.7
MS.gene20277.t1 MTR_8g093070 24.648 426 243 15 4 417 139 498 2.67e-17 84.3
MS.gene20277.t1 MTR_2g097380 22.222 414 234 15 11 417 231 563 1.51e-16 82.0
MS.gene20277.t1 MTR_1g096030 23.462 439 248 15 4 414 80 458 8.18e-16 79.3
MS.gene20277.t1 MTR_7g109580 23.529 323 198 9 98 414 274 553 1.15e-14 76.3
MS.gene20277.t1 MTR_3g072710 22.222 324 210 7 95 414 214 499 8.17e-14 73.6
MS.gene20277.t1 MTR_5g058860 24.113 423 264 16 4 414 119 496 8.59e-14 73.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20277.t1 AT3G49950 57.995 419 137 8 1 416 27 409 1.37e-165 471
MS.gene20277.t1 AT5G67411 55.066 227 75 4 132 356 1 202 6.61e-76 235
MS.gene20277.t1 AT4G37650 27.442 430 256 10 2 417 144 531 7.38e-41 152
MS.gene20277.t1 AT2G04890 25.000 416 257 7 1 414 50 412 1.50e-34 133
MS.gene20277.t1 AT1G21450 26.005 423 250 9 2 414 223 592 3.69e-34 134
MS.gene20277.t1 AT5G48150 26.747 415 251 8 5 414 123 489 3.85e-33 130
MS.gene20277.t1 AT5G48150 26.747 415 251 8 5 414 123 489 3.85e-33 130
MS.gene20277.t1 AT5G48150 26.747 415 251 8 5 414 123 489 3.85e-33 130
MS.gene20277.t1 AT5G48150 26.747 415 251 8 5 414 123 489 3.85e-33 130
MS.gene20277.t1 AT5G17490 25.604 414 249 6 3 414 159 515 1.63e-32 129
MS.gene20277.t1 AT1G50600 24.821 419 261 10 1 414 156 525 1.90e-32 129
MS.gene20277.t1 AT1G50600 24.821 419 261 10 1 414 227 596 3.60e-32 129
MS.gene20277.t1 AT1G50600 24.821 419 261 10 1 414 231 600 4.09e-32 128
MS.gene20277.t1 AT3G03450 25.728 412 253 8 5 414 184 544 1.27e-31 126
MS.gene20277.t1 AT4G17230 24.648 426 257 9 1 416 155 526 2.97e-30 122
MS.gene20277.t1 AT4G17230 24.648 426 257 9 1 416 155 526 2.97e-30 122
MS.gene20277.t1 AT2G29065 25.776 419 266 12 4 417 252 630 3.31e-29 120
MS.gene20277.t1 AT3G13840 26.651 424 240 14 2 414 146 509 1.08e-27 115
MS.gene20277.t1 AT1G14920 24.096 415 251 12 5 414 173 528 8.29e-26 109
MS.gene20277.t1 AT2G01570 23.729 413 255 12 5 414 225 580 4.46e-25 107
MS.gene20277.t1 AT1G50420 24.561 456 265 14 4 414 57 478 4.51e-25 107
MS.gene20277.t1 AT5G52510 22.970 431 255 11 3 414 267 639 4.18e-24 105
MS.gene20277.t1 AT2G37650 23.697 422 265 14 1 416 332 702 4.64e-24 105
MS.gene20277.t1 AT2G37650 23.697 422 265 14 1 416 344 714 6.14e-24 104
MS.gene20277.t1 AT1G66350 23.002 413 252 8 5 414 156 505 7.04e-23 101
MS.gene20277.t1 AT3G46600 22.695 423 271 9 1 417 1 373 2.78e-22 98.2
MS.gene20277.t1 AT1G55580 23.690 439 275 12 1 414 41 444 1.22e-21 97.1
MS.gene20277.t1 AT3G46600 22.770 426 267 9 1 417 177 549 2.06e-21 97.1
MS.gene20277.t1 AT3G46600 23.005 426 266 9 1 417 209 581 2.33e-21 96.7
MS.gene20277.t1 AT1G07520 23.963 434 254 17 4 417 154 531 3.09e-21 96.3
MS.gene20277.t1 AT1G07520 23.963 434 254 17 4 417 154 531 3.09e-21 96.3
MS.gene20277.t1 AT1G07520 23.963 434 254 17 4 417 319 696 5.02e-21 95.9
MS.gene20277.t1 AT1G07520 23.963 434 254 17 4 417 318 695 5.85e-21 95.9
MS.gene20277.t1 AT5G59450 23.113 424 272 10 1 417 224 600 9.34e-21 95.1
MS.gene20277.t1 AT1G63100 23.185 427 262 13 4 414 277 653 1.11e-19 92.0
MS.gene20277.t1 AT1G63100 23.185 427 262 13 4 414 252 628 1.34e-19 91.7
MS.gene20277.t1 AT1G07530 20.665 421 284 9 1 417 393 767 4.67e-19 90.1
MS.gene20277.t1 AT2G29060 22.877 424 271 12 1 416 318 693 6.44e-19 89.7
MS.gene20277.t1 AT2G29060 22.877 424 271 12 1 416 281 656 6.52e-19 89.7
MS.gene20277.t1 AT5G41920 23.022 417 255 14 4 414 43 399 6.56e-18 85.5
MS.gene20277.t1 AT5G66770 25.685 292 178 7 95 383 294 549 1.47e-17 85.1
MS.gene20277.t1 AT3G50650 24.699 332 200 9 95 414 248 541 4.30e-13 71.2
MS.gene20277.t1 AT4G08250 23.611 432 247 18 4 414 107 476 3.17e-11 65.5

Find 62 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCTTTCTTACGACGAATT+AGG 0.219981 5.1:+8311544 MS.gene20277:CDS
CGACGAATTAGGTTCAAAAT+TGG 0.230348 5.1:+8311555 MS.gene20277:CDS
AATCCGCGTTTAATTATCTT+TGG 0.234805 5.1:+8311923 MS.gene20277:CDS
GCAGCTGTTAGCTTGATTAA+AGG 0.266223 5.1:-8311466 None:intergenic
TTTCAGTGAGGATTCGGTTT+TGG 0.295734 5.1:+8312131 MS.gene20277:CDS
ATGAGCTTCTTGTGCTTACT+TGG 0.341746 5.1:+8312208 MS.gene20277:CDS
TAAAACAAGAAACATGATTA+TGG 0.351765 5.1:+8311588 MS.gene20277:CDS
AATGTAAATAGAGTTTCTTC+AGG 0.361286 5.1:-8311745 None:intergenic
AGATTTAACATCAAGTAGTT+TGG 0.367518 5.1:+8311888 MS.gene20277:CDS
AGATCAAGCATTGGTGGAAC+TGG 0.372508 5.1:-8311526 None:intergenic
GCATTGGTGGAACTGGAATA+TGG 0.373190 5.1:-8311519 None:intergenic
CACTAGCACAACAAATCCTT+TGG 0.378101 5.1:+8311074 MS.gene20277:CDS
GCTTCCTGCTAGGCGTTGAT+TGG 0.395425 5.1:-8311131 None:intergenic
TTAACACCTTGGCATCGTTT+CGG 0.442385 5.1:+8311307 MS.gene20277:CDS
TCCACCAAGACGACTATTGT+TGG 0.444785 5.1:-8311856 None:intergenic
TCTCATTCGTTGCTCCCACT+TGG 0.447629 5.1:-8312085 None:intergenic
ATGATAGTTATAACTATTTG+CGG 0.450034 5.1:-8311224 None:intergenic
AGTTTGTTTCAGTGAGGATT+CGG 0.453807 5.1:+8312125 MS.gene20277:CDS
TAATAAATCTCATGAAGCAT+TGG 0.465324 5.1:+8311690 MS.gene20277:CDS
CTGAGGGTGTTGTGTTGTTA+AGG 0.465694 5.1:-8311099 None:intergenic
GGTATGAAGCTGAAATTTGT+TGG 0.481427 5.1:+8312001 MS.gene20277:CDS
GCAATTCTAGAAGCTACTAA+AGG 0.483791 5.1:+8311349 MS.gene20277:CDS
ATGTTATGTTTGCTTCTGCT+TGG 0.495765 5.1:+8312241 MS.gene20277:CDS
AGAAAACTCGTGTGTTTGTA+TGG 0.500579 5.1:-8311257 None:intergenic
CTTCTTGTGCTTACTTGGAA+AGG 0.503343 5.1:+8312213 MS.gene20277:CDS
GAGAATGTGATAGCATATGA+AGG 0.509915 5.1:+8312030 MS.gene20277:CDS
TTCTGGAATATTGCAACTAC+AGG 0.513561 5.1:-8311188 None:intergenic
TGATGAACATGCAGCTGGAT+GGG 0.514109 5.1:+8312170 MS.gene20277:CDS
ATTGTTGCTTTCAATTGCAC+TGG 0.516587 5.1:-8311047 None:intergenic
GTCTCCAATCAACGCCTAGC+AGG 0.519304 5.1:+8311127 MS.gene20277:CDS
AGATCCAACAATAGTCGTCT+TGG 0.519549 5.1:+8311852 MS.gene20277:CDS
TCCAACAATAGTCGTCTTGG+TGG 0.526010 5.1:+8311855 MS.gene20277:CDS
AATGTGTCCACCGTGTCAAA+CGG 0.536257 5.1:-8311949 None:intergenic
ACTAGCACAACAAATCCTTT+GGG 0.538819 5.1:+8311075 MS.gene20277:CDS
ATGCTTGATGAACATGCAGC+TGG 0.552523 5.1:+8312165 MS.gene20277:CDS
GTGGGAGCAACGAATGAGAA+AGG 0.554477 5.1:+8312089 MS.gene20277:CDS
GATGAACATGCAGCTGGATG+GGG 0.559473 5.1:+8312171 MS.gene20277:CDS
TTGATTGGAGACATCGTCTG+AGG 0.562823 5.1:-8311116 None:intergenic
GTTTCTTCAACTTATTCAGA+CGG 0.564374 5.1:+8311622 MS.gene20277:CDS
TAAGAAAGATCAAGCATTGG+TGG 0.570387 5.1:-8311532 None:intergenic
CTCGAATGAAGCTTCCTGCT+AGG 0.577569 5.1:-8311141 None:intergenic
TTGATGAACATGCAGCTGGA+TGG 0.579446 5.1:+8312169 MS.gene20277:CDS
TTGTGTTGTTAAGGACCCAA+AGG 0.585571 5.1:-8311090 None:intergenic
GCAATGGCGTCGATTAGTGT+AGG 0.586251 5.1:-8311424 None:intergenic
TGCCTTTGTTTACTCCCTCG+TGG 0.588128 5.1:-8311976 None:intergenic
GCTGTTAGCTTGATTAAAGG+AGG 0.610301 5.1:-8311463 None:intergenic
AACCTCATGATGATTTGCAA+TGG 0.610435 5.1:-8311440 None:intergenic
CAGCTGCAGTTAATAACTGT+AGG 0.611302 5.1:+8311482 MS.gene20277:CDS
AATGTTGCACTTGCACTCGT+AGG 0.611711 5.1:-8311666 None:intergenic
AGAATGTGATAGCATATGAA+GGG 0.615697 5.1:+8312031 MS.gene20277:CDS
CGCCATTGCAAATCATCATG+AGG 0.616800 5.1:+8311438 MS.gene20277:CDS
TTGGATACCGTTTGACACGG+TGG 0.625357 5.1:+8311942 MS.gene20277:CDS
TCTTTGGATACCGTTTGACA+CGG 0.625561 5.1:+8311939 MS.gene20277:CDS
TGATTGGAGACATCGTCTGA+GGG 0.630764 5.1:-8311115 None:intergenic
GAGGGAGTAAACAAAGGCAG+TGG 0.640189 5.1:+8311980 MS.gene20277:CDS
TCAAGGAGTTTGTTTCAGTG+AGG 0.641089 5.1:+8312119 MS.gene20277:CDS
TCGTAAGAAAGATCAAGCAT+TGG 0.656089 5.1:-8311535 None:intergenic
GAGTCGAGTCTAAAACCAAG+TGG 0.658958 5.1:+8312070 MS.gene20277:CDS
AGTCGAGTCTAAAACCAAGT+GGG 0.662079 5.1:+8312071 MS.gene20277:CDS
TACCACGAGGGAGTAAACAA+AGG 0.668759 5.1:+8311974 MS.gene20277:CDS
GTGTAACCGAAACGATGCCA+AGG 0.704462 5.1:-8311313 None:intergenic
TATCCAAAGATAATTAAACG+CGG 0.789557 5.1:-8311926 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGATAGTTATAACTATTTG+CGG - chr5.1:8311227-8311246 None:intergenic 20.0%
!! TAAAACAAGAAACATGATTA+TGG + chr5.1:8311588-8311607 MS.gene20277:CDS 20.0%
!!! ATTTTATTGATTTAACACCT+TGG + chr5.1:8311296-8311315 MS.gene20277:CDS 20.0%
! AATGTAAATAGAGTTTCTTC+AGG - chr5.1:8311748-8311767 None:intergenic 25.0%
! AGATTTAACATCAAGTAGTT+TGG + chr5.1:8311888-8311907 MS.gene20277:CDS 25.0%
! TAATAAATCTCATGAAGCAT+TGG + chr5.1:8311690-8311709 MS.gene20277:CDS 25.0%
! TATCCAAAGATAATTAAACG+CGG - chr5.1:8311929-8311948 None:intergenic 25.0%
AATCCGCGTTTAATTATCTT+TGG + chr5.1:8311923-8311942 MS.gene20277:CDS 30.0%
AGAATGTGATAGCATATGAA+GGG + chr5.1:8312031-8312050 MS.gene20277:CDS 30.0%
GTTTCTTCAACTTATTCAGA+CGG + chr5.1:8311622-8311641 MS.gene20277:CDS 30.0%
ACTAGCACAACAAATCCTTT+GGG + chr5.1:8311075-8311094 MS.gene20277:CDS 35.0%
AGAAAACTCGTGTGTTTGTA+TGG - chr5.1:8311260-8311279 None:intergenic 35.0%
ATGTTATGTTTGCTTCTGCT+TGG + chr5.1:8312241-8312260 MS.gene20277:CDS 35.0%
CGACGAATTAGGTTCAAAAT+TGG + chr5.1:8311555-8311574 MS.gene20277:CDS 35.0%
GAGAATGTGATAGCATATGA+AGG + chr5.1:8312030-8312049 MS.gene20277:CDS 35.0%
GATCTTTCTTACGACGAATT+AGG + chr5.1:8311544-8311563 MS.gene20277:CDS 35.0%
GGTATGAAGCTGAAATTTGT+TGG + chr5.1:8312001-8312020 MS.gene20277:CDS 35.0%
TCGTAAGAAAGATCAAGCAT+TGG - chr5.1:8311538-8311557 None:intergenic 35.0%
! AACCTCATGATGATTTGCAA+TGG - chr5.1:8311443-8311462 None:intergenic 35.0%
! AGTTTGTTTCAGTGAGGATT+CGG + chr5.1:8312125-8312144 MS.gene20277:CDS 35.0%
! GCAATTCTAGAAGCTACTAA+AGG + chr5.1:8311349-8311368 MS.gene20277:CDS 35.0%
!! ATTGTTGCTTTCAATTGCAC+TGG - chr5.1:8311050-8311069 None:intergenic 35.0%
!! TAAGAAAGATCAAGCATTGG+TGG - chr5.1:8311535-8311554 None:intergenic 35.0%
!! TTCTGGAATATTGCAACTAC+AGG - chr5.1:8311191-8311210 None:intergenic 35.0%
!!! CGATGTTTTTAGAAGCTGTA+CGG + chr5.1:8311824-8311843 MS.gene20277:CDS 35.0%
AGATCCAACAATAGTCGTCT+TGG + chr5.1:8311852-8311871 MS.gene20277:CDS 40.0%
ATGAGCTTCTTGTGCTTACT+TGG + chr5.1:8312208-8312227 MS.gene20277:CDS 40.0%
CACTAGCACAACAAATCCTT+TGG + chr5.1:8311074-8311093 MS.gene20277:CDS 40.0%
CAGCTGCAGTTAATAACTGT+AGG + chr5.1:8311482-8311501 MS.gene20277:CDS 40.0%
CTTCTTGTGCTTACTTGGAA+AGG + chr5.1:8312213-8312232 MS.gene20277:CDS 40.0%
TCTTTGGATACCGTTTGACA+CGG + chr5.1:8311939-8311958 MS.gene20277:CDS 40.0%
TTAACACCTTGGCATCGTTT+CGG + chr5.1:8311307-8311326 MS.gene20277:CDS 40.0%
! AGTCGAGTCTAAAACCAAGT+GGG + chr5.1:8312071-8312090 MS.gene20277:CDS 40.0%
! CGAGTTTTCTGTCATTGAGT+TGG + chr5.1:8311270-8311289 MS.gene20277:CDS 40.0%
! GCAGCTGTTAGCTTGATTAA+AGG - chr5.1:8311469-8311488 None:intergenic 40.0%
! GCTGTTAGCTTGATTAAAGG+AGG - chr5.1:8311466-8311485 None:intergenic 40.0%
! TCAAGGAGTTTGTTTCAGTG+AGG + chr5.1:8312119-8312138 MS.gene20277:CDS 40.0%
! TGGACACATTTTTACCACGA+GGG + chr5.1:8311962-8311981 MS.gene20277:CDS 40.0%
! TTGTGTTGTTAAGGACCCAA+AGG - chr5.1:8311093-8311112 None:intergenic 40.0%
!! ATGAGAAAGGCACGTTTTCA+AGG + chr5.1:8312102-8312121 MS.gene20277:CDS 40.0%
!! TTTCAGTGAGGATTCGGTTT+TGG + chr5.1:8312131-8312150 MS.gene20277:CDS 40.0%
!!! GATTCGGTTTTGGAGGTTAA+GGG + chr5.1:8312141-8312160 MS.gene20277:CDS 40.0%
AATGTGTCCACCGTGTCAAA+CGG - chr5.1:8311952-8311971 None:intergenic 45.0%
AATGTTGCACTTGCACTCGT+AGG - chr5.1:8311669-8311688 None:intergenic 45.0%
ATGCTTGATGAACATGCAGC+TGG + chr5.1:8312165-8312184 MS.gene20277:CDS 45.0%
CGCCATTGCAAATCATCATG+AGG + chr5.1:8311438-8311457 MS.gene20277:CDS 45.0%
TACCACGAGGGAGTAAACAA+AGG + chr5.1:8311974-8311993 MS.gene20277:CDS 45.0%
TCCAACAATAGTCGTCTTGG+TGG + chr5.1:8311855-8311874 MS.gene20277:CDS 45.0%
TCCACCAAGACGACTATTGT+TGG - chr5.1:8311859-8311878 None:intergenic 45.0%
TGATGAACATGCAGCTGGAT+GGG + chr5.1:8312170-8312189 MS.gene20277:CDS 45.0%
TGATTGGAGACATCGTCTGA+GGG - chr5.1:8311118-8311137 None:intergenic 45.0%
TTGATGAACATGCAGCTGGA+TGG + chr5.1:8312169-8312188 MS.gene20277:CDS 45.0%
TTGATTGGAGACATCGTCTG+AGG - chr5.1:8311119-8311138 None:intergenic 45.0%
! GAGTCGAGTCTAAAACCAAG+TGG + chr5.1:8312070-8312089 MS.gene20277:CDS 45.0%
! GCATTGGTGGAACTGGAATA+TGG - chr5.1:8311522-8311541 None:intergenic 45.0%
! GCGGTTTTTGTAACTGCTTC+TGG - chr5.1:8311208-8311227 None:intergenic 45.0%
! GTGGACACATTTTTACCACG+AGG + chr5.1:8311961-8311980 MS.gene20277:CDS 45.0%
!! AGATCAAGCATTGGTGGAAC+TGG - chr5.1:8311529-8311548 None:intergenic 45.0%
!! CTGAGGGTGTTGTGTTGTTA+AGG - chr5.1:8311102-8311121 None:intergenic 45.0%
!!! GGATTCGGTTTTGGAGGTTA+AGG + chr5.1:8312140-8312159 MS.gene20277:CDS 45.0%
CTCGAATGAAGCTTCCTGCT+AGG - chr5.1:8311144-8311163 None:intergenic 50.0%
GAGGGAGTAAACAAAGGCAG+TGG + chr5.1:8311980-8311999 MS.gene20277:CDS 50.0%
GATGAACATGCAGCTGGATG+GGG + chr5.1:8312171-8312190 MS.gene20277:CDS 50.0%
GTGGGAGCAACGAATGAGAA+AGG + chr5.1:8312089-8312108 MS.gene20277:CDS 50.0%
GTGTAACCGAAACGATGCCA+AGG - chr5.1:8311316-8311335 None:intergenic 50.0%
TCTCATTCGTTGCTCCCACT+TGG - chr5.1:8312088-8312107 None:intergenic 50.0%
TTGGATACCGTTTGACACGG+TGG + chr5.1:8311942-8311961 MS.gene20277:CDS 50.0%
! TGCCTTTGTTTACTCCCTCG+TGG - chr5.1:8311979-8311998 None:intergenic 50.0%
!! CAGTGAGGATTCGGTTTTGG+AGG + chr5.1:8312134-8312153 MS.gene20277:CDS 50.0%
!! GCAATGGCGTCGATTAGTGT+AGG - chr5.1:8311427-8311446 None:intergenic 50.0%
GTCTCCAATCAACGCCTAGC+AGG + chr5.1:8311127-8311146 MS.gene20277:CDS 55.0%
!! GCTTCCTGCTAGGCGTTGAT+TGG - chr5.1:8311134-8311153 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 8311022 8312275 8311022 ID=MS.gene20277
chr5.1 mRNA 8311022 8312275 8311022 ID=MS.gene20277.t1;Parent=MS.gene20277
chr5.1 exon 8311022 8312275 8311022 ID=MS.gene20277.t1.exon1;Parent=MS.gene20277.t1
chr5.1 CDS 8311022 8312275 8311022 ID=cds.MS.gene20277.t1;Parent=MS.gene20277.t1
Gene Sequence

>MS.gene20277

ATGGAGCAGTTACTCATTCACTGTGCCAGTGCAATTGAAAGCAACAATGCAACACTAGCACAACAAATCCTTTGGGTCCTTAACAACACAACACCCTCAGACGATGTCTCCAATCAACGCCTAGCAGGAAGCTTCATTCGAGCTCTTACAACGCGTGCAAGAAGATCCTGTAGTTGCAATATTCCAGAAGCAGTTACAAAAACCGCAAATAGTTATAACTATCATCACAATCTCGCCATACAAACACACGAGTTTTCTGTCATTGAGTTGGCAAATTTTATTGATTTAACACCTTGGCATCGTTTCGGTTACACAGCAGCTAATTCTGCAATTCTAGAAGCTACTAAAGGATTTTCAGTTATTCACATTGTTGATTTGAGTTTAACACACTGCATGCAGATTCCTACACTAATCGACGCCATTGCAAATCATCATGAGGTTCCTCCTTTAATCAAGCTAACAGCTGCAGTTAATAACTGTAGGAATAAGAATATTAACCATATTCCAGTTCCACCAATGCTTGATCTTTCTTACGACGAATTAGGTTCAAAATTGGTTAATTTTGCTAAAACAAGAAACATGATTATGGAATTCAAAGCTGTTTCTTCAACTTATTCAGACGGATTTGCTTCGTTGATTGAACACCTACGAGTGCAAGTGCAACATTTTAATAAATCTCATGAAGCATTGGTTATAAACTGTCACATGATGCTTCATTATATTCCTGAAGAAACTCTATTTACATTTAATGATACAAATTCAAATTTGAATTTGAATTTAAACTCTTGTTCATCGCTGCGCTCGATGTTTTTAGAAGCTGTACGGAGTTTAGATCCAACAATAGTCGTCTTGGTGGATGAAGATGCAGATTTAACATCAAGTAGTTTGGTTAGTAGACTAAAATCCGCGTTTAATTATCTTTGGATACCGTTTGACACGGTGGACACATTTTTACCACGAGGGAGTAAACAAAGGCAGTGGTATGAAGCTGAAATTTGTTGGAAGATTGAGAATGTGATAGCATATGAAGGGATTCAGAGAGTTGAGAGAGTCGAGTCTAAAACCAAGTGGGAGCAACGAATGAGAAAGGCACGTTTTCAAGGAGTTTGTTTCAGTGAGGATTCGGTTTTGGAGGTTAAGGGTATGCTTGATGAACATGCAGCTGGATGGGGATTGAAGAAAGAAGATGAGCTTCTTGTGCTTACTTGGAAAGGACATAATGTTATGTTTGCTTCTGCTTGGTTACCTTCTTGA
Protein sequence

>MS.gene20277.t1

MEQLLIHCASAIESNNATLAQQILWVLNNTTPSDDVSNQRLAGSFIRALTTRARRSCSCNIPEAVTKTANSYNYHHNLAIQTHEFSVIELANFIDLTPWHRFGYTAANSAILEATKGFSVIHIVDLSLTHCMQIPTLIDAIANHHEVPPLIKLTAAVNNCRNKNINHIPVPPMLDLSYDELGSKLVNFAKTRNMIMEFKAVSSTYSDGFASLIEHLRVQVQHFNKSHEALVINCHMMLHYIPEETLFTFNDTNSNLNLNLNSCSSLRSMFLEAVRSLDPTIVVLVDEDADLTSSSLVSRLKSAFNYLWIPFDTVDTFLPRGSKQRQWYEAEICWKIENVIAYEGIQRVERVESKTKWEQRMRKARFQGVCFSEDSVLEVKGMLDEHAAGWGLKKEDELLVLTWKGHNVMFASAWLPS