AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20299

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20299.t1 MTR_5g015490 97.460 433 9 1 1 431 59 491 0.0 877
MS.gene20299.t1 MTR_4g097080 69.575 447 109 10 2 431 68 504 0.0 611
MS.gene20299.t1 MTR_2g099110 54.545 396 155 7 50 431 68 452 1.53e-144 420
MS.gene20299.t1 MTR_2g089100 31.106 434 270 7 21 431 17 444 5.55e-61 205
MS.gene20299.t1 MTR_5g097480 29.744 390 253 7 48 430 169 544 1.40e-54 190
MS.gene20299.t1 MTR_2g082090 31.759 381 241 7 56 430 212 579 2.42e-54 190
MS.gene20299.t1 MTR_1g029420 30.971 381 246 8 55 430 224 592 2.80e-54 190
MS.gene20299.t1 MTR_5g094450 30.548 383 247 7 56 430 161 532 7.63e-53 185
MS.gene20299.t1 MTR_3g089055 30.971 381 246 8 55 430 202 570 6.09e-51 181
MS.gene20299.t1 MTR_3g089055 30.971 381 246 8 55 430 197 565 8.91e-51 180
MS.gene20299.t1 MTR_4g095500 28.446 457 280 10 17 431 18 469 9.99e-51 178
MS.gene20299.t1 MTR_3g056110 29.383 405 240 12 51 430 159 542 1.76e-49 176
MS.gene20299.t1 MTR_5g015950 28.217 443 258 12 36 431 18 447 1.61e-47 169
MS.gene20299.t1 MTR_3g053270 28.217 443 258 12 36 431 18 447 1.61e-47 169
MS.gene20299.t1 MTR_3g065980 28.228 457 276 9 3 430 107 540 2.79e-46 167
MS.gene20299.t1 MTR_7g027190 30.982 397 231 12 55 430 299 673 3.98e-46 169
MS.gene20299.t1 MTR_2g026250 29.167 360 234 8 78 430 251 596 2.50e-44 163
MS.gene20299.t1 MTR_4g133660 28.906 384 247 10 56 429 175 542 2.27e-43 159
MS.gene20299.t1 MTR_8g020840 31.188 404 235 13 50 430 165 548 1.07e-42 158
MS.gene20299.t1 MTR_4g102790 27.720 386 257 11 55 431 291 663 6.90e-41 154
MS.gene20299.t1 MTR_8g442410 29.211 380 253 9 57 430 156 525 8.89e-41 152
MS.gene20299.t1 MTR_7g074650 28.307 378 241 13 59 431 441 793 5.67e-40 152
MS.gene20299.t1 MTR_4g064200 28.796 382 252 9 56 431 284 651 1.21e-39 150
MS.gene20299.t1 MTR_4g077760 29.610 385 237 11 57 430 194 555 4.07e-37 142
MS.gene20299.t1 MTR_4g064150 26.579 380 271 4 55 431 360 734 4.24e-37 144
MS.gene20299.t1 MTR_2g097310 26.316 380 264 9 56 431 272 639 3.36e-36 140
MS.gene20299.t1 MTR_2g097410 28.272 382 258 7 56 431 371 742 9.72e-36 140
MS.gene20299.t1 MTR_4g064120 27.920 351 241 7 55 400 253 596 2.76e-35 138
MS.gene20299.t1 MTR_4g064180 27.920 351 241 7 55 400 253 596 1.06e-33 133
MS.gene20299.t1 MTR_4g064160 26.649 379 266 5 56 431 316 685 1.15e-33 134
MS.gene20299.t1 MTR_7g062120 25.066 379 272 5 56 431 372 741 1.29e-33 134
MS.gene20299.t1 MTR_2g097350 25.263 380 268 8 56 431 274 641 2.76e-33 132
MS.gene20299.t1 MTR_2g097467 25.393 382 264 7 56 431 289 655 3.73e-33 132
MS.gene20299.t1 MTR_4g104020 25.641 390 259 10 55 430 148 520 3.80e-33 131
MS.gene20299.t1 MTR_2g097390 26.053 380 265 9 56 431 275 642 1.44e-32 130
MS.gene20299.t1 MTR_2g097463 25.916 382 262 7 56 431 290 656 5.80e-32 129
MS.gene20299.t1 MTR_2g097473 25.654 382 263 7 56 431 289 655 1.14e-31 127
MS.gene20299.t1 MTR_3g022830 23.529 425 260 9 57 430 26 436 7.93e-31 123
MS.gene20299.t1 MTR_4g076020 26.720 378 238 14 59 431 87 430 2.61e-30 122
MS.gene20299.t1 MTR_1g069725 27.034 381 244 14 56 429 109 462 7.42e-29 118
MS.gene20299.t1 MTR_3g072710 27.407 405 240 15 55 429 119 499 9.37e-27 112
MS.gene20299.t1 MTR_5g009080 28.819 288 183 7 1 283 5 275 7.83e-26 109
MS.gene20299.t1 MTR_4g076140 29.960 247 163 4 36 281 28 265 1.06e-25 109
MS.gene20299.t1 MTR_1g096030 26.847 406 242 14 55 430 79 459 1.78e-25 108
MS.gene20299.t1 MTR_2g034260 24.479 384 270 8 57 430 211 584 1.01e-24 107
MS.gene20299.t1 MTR_8g093070 26.804 388 245 17 55 431 138 497 2.24e-24 105
MS.gene20299.t1 MTR_2g097380 25.926 324 213 8 59 380 227 525 4.18e-24 105
MS.gene20299.t1 MTR_5g058860 27.525 396 249 14 55 429 118 496 4.63e-23 101
MS.gene20299.t1 MTR_2g034280 25.000 388 261 12 57 430 204 575 8.29e-23 101
MS.gene20299.t1 MTR_7g057230 24.570 407 280 9 34 429 266 656 1.28e-21 97.8
MS.gene20299.t1 MTR_2g034250 23.636 385 273 8 57 430 211 585 3.02e-21 96.7
MS.gene20299.t1 MTR_6g047750 26.943 386 251 13 57 430 296 662 3.11e-20 93.6
MS.gene20299.t1 MTR_1g086970 25.691 362 246 12 78 430 131 478 2.15e-19 90.5
MS.gene20299.t1 MTR_7g109580 24.138 377 271 8 57 430 190 554 4.28e-18 87.0
MS.gene20299.t1 MTR_4g026485 21.277 376 273 8 57 430 270 624 3.95e-16 80.9
MS.gene20299.t1 MTR_0092s0100 21.579 380 268 8 57 430 373 728 1.80e-13 72.8
MS.gene20299.t1 MTR_0092s0100 21.579 380 268 8 57 430 376 731 1.97e-13 72.8
MS.gene20299.t1 MTR_8g077940 22.597 385 256 10 54 430 191 541 2.50e-12 68.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20299.t1 AT4G37650 65.625 416 125 7 29 431 120 530 0.0 550
MS.gene20299.t1 AT2G04890 31.383 376 242 7 56 430 53 413 4.87e-59 199
MS.gene20299.t1 AT1G21450 32.642 386 234 9 55 430 224 593 3.48e-51 182
MS.gene20299.t1 AT3G49950 31.566 396 247 9 50 431 24 409 4.19e-51 177
MS.gene20299.t1 AT5G48150 28.382 377 258 6 57 430 123 490 7.49e-51 179
MS.gene20299.t1 AT5G48150 28.382 377 258 6 57 430 123 490 7.49e-51 179
MS.gene20299.t1 AT5G48150 28.382 377 258 6 57 430 123 490 7.49e-51 179
MS.gene20299.t1 AT5G48150 28.382 377 258 6 57 430 123 490 7.49e-51 179
MS.gene20299.t1 AT1G50600 30.208 384 243 9 56 430 159 526 1.43e-49 176
MS.gene20299.t1 AT1G50600 30.208 384 243 9 56 430 230 597 4.42e-49 176
MS.gene20299.t1 AT1G50600 30.208 384 243 9 56 430 234 601 5.90e-49 176
MS.gene20299.t1 AT3G03450 30.311 386 249 7 49 430 176 545 4.35e-48 172
MS.gene20299.t1 AT1G66350 30.626 431 269 12 3 430 103 506 1.62e-47 170
MS.gene20299.t1 AT5G17490 31.525 387 243 8 46 430 150 516 5.73e-47 169
MS.gene20299.t1 AT2G01570 29.178 377 244 10 57 430 225 581 5.14e-42 157
MS.gene20299.t1 AT1G14920 29.708 377 242 9 57 430 173 529 8.01e-41 152
MS.gene20299.t1 AT4G17230 26.110 383 262 8 56 431 158 526 2.77e-40 151
MS.gene20299.t1 AT4G17230 26.110 383 262 8 56 431 158 526 2.77e-40 151
MS.gene20299.t1 AT1G50420 25.319 470 281 7 21 430 20 479 3.80e-40 150
MS.gene20299.t1 AT3G13840 31.061 396 231 14 50 430 142 510 1.31e-38 146
MS.gene20299.t1 AT3G54220 28.609 381 239 14 59 431 296 651 1.65e-37 144
MS.gene20299.t1 AT1G63100 29.156 391 248 11 55 429 276 653 4.31e-37 143
MS.gene20299.t1 AT1G63100 29.156 391 248 11 55 429 251 628 6.24e-37 143
MS.gene20299.t1 AT5G41920 28.982 383 241 14 55 430 42 400 2.29e-34 132
MS.gene20299.t1 AT1G55580 28.155 412 249 11 56 430 44 445 1.00e-33 131
MS.gene20299.t1 AT3G46600 27.297 381 262 8 55 431 3 372 2.10e-33 129
MS.gene20299.t1 AT2G29065 26.234 385 268 9 56 431 252 629 4.38e-33 132
MS.gene20299.t1 AT5G66770 27.926 376 244 10 64 430 227 584 7.57e-33 130
MS.gene20299.t1 AT2G37650 26.053 380 267 6 55 431 346 714 1.11e-32 131
MS.gene20299.t1 AT2G37650 26.053 380 267 6 55 431 334 702 1.36e-32 130
MS.gene20299.t1 AT3G46600 27.297 381 262 8 55 431 211 580 2.17e-32 129
MS.gene20299.t1 AT3G46600 27.297 381 262 8 55 431 179 548 2.26e-32 129
MS.gene20299.t1 AT5G52510 27.041 392 248 9 57 430 269 640 4.11e-32 129
MS.gene20299.t1 AT1G07530 25.263 380 271 6 56 431 396 766 6.73e-32 129
MS.gene20299.t1 AT5G59450 26.371 383 267 7 55 431 226 599 4.95e-29 120
MS.gene20299.t1 AT2G29060 24.869 382 272 8 56 431 321 693 5.11e-29 120
MS.gene20299.t1 AT2G29060 24.869 382 272 8 56 431 284 656 5.18e-29 120
MS.gene20299.t1 AT1G07520 24.869 382 276 7 56 431 319 695 6.54e-29 120
MS.gene20299.t1 AT1G07520 24.869 382 276 7 56 431 318 694 7.09e-29 119
MS.gene20299.t1 AT3G50650 28.680 394 239 12 54 430 174 542 7.52e-29 119
MS.gene20299.t1 AT1G07520 24.869 382 276 7 56 431 154 530 7.68e-29 119
MS.gene20299.t1 AT1G07520 24.869 382 276 7 56 431 154 530 7.68e-29 119
MS.gene20299.t1 AT4G08250 26.764 411 252 17 40 429 94 476 3.82e-23 102
MS.gene20299.t1 AT4G00150 23.256 387 242 12 58 430 209 554 1.75e-12 69.7
MS.gene20299.t1 AT3G60630 24.919 309 199 12 136 430 333 622 2.68e-12 69.3

Find 111 sgRNAs with CRISPR-Local

Find 128 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGGAGGCTGATTTAAATTT+TGG 0.142361 5.1:-7962600 MS.gene20299:CDS
TGAATAACGTTGAATTTAAA+TGG 0.226774 5.1:+7962810 None:intergenic
GATTTCACCTTTGAATTAAC+TGG 0.256797 5.1:-7963362 MS.gene20299:CDS
AAGAGTGTTTGAGATGGTTT+AGG 0.270052 5.1:-7962545 MS.gene20299:CDS
GGCTTTGTTGAGGAGGTATA+AGG 0.272310 5.1:-7962307 MS.gene20299:CDS
TTCGGTCATTCGAGAGAATA+AGG 0.284815 5.1:+7963190 None:intergenic
ACCTTCGAAGGCTTCTAAAA+TGG 0.301815 5.1:+7963031 None:intergenic
GGGGTGTGAGTGATGGTGTG+GGG 0.332972 5.1:+7963399 None:intergenic
TGGGAGAGGGATAGTTGATT+TGG 0.342461 5.1:-7962451 MS.gene20299:CDS
GGGTGTGAGTGATGGTGTGG+GGG 0.342781 5.1:+7963400 None:intergenic
AAATCAGTTGTTGGTGGAGG+GGG 0.353733 5.1:+7963380 None:intergenic
AGGAAGTTTGTGATGATGTT+AGG 0.369294 5.1:-7962329 MS.gene20299:CDS
GAAGGTTCTGGTACCGGCTT+CGG 0.378697 5.1:+7963172 None:intergenic
AGAGTGTTTGAGATGGTTTA+GGG 0.380120 5.1:-7962544 MS.gene20299:CDS
GAGGTTTGAGGTTGACTATA+AGG 0.384294 5.1:+7963443 None:intergenic
TAGTCCATGGACTAACTTCT+TGG 0.385254 5.1:+7963081 None:intergenic
CGTTGATCGCGTTGCGGCCG+AGG 0.391480 5.1:-7962641 MS.gene20299:CDS
CTTCCACGTCAAGAATATTC+CGG 0.409628 5.1:+7962254 None:intergenic
GTTAGTCCATGGACTACTTT+CGG 0.413327 5.1:-7963074 MS.gene20299:CDS
AGCTAGGGTTCTGAAGGTTC+TGG 0.415747 5.1:+7963160 None:intergenic
GTTTGTAGTAATGGTGTTTG+AGG 0.420379 5.1:+7963424 None:intergenic
ACTATAAGGATAGTAGTGTT+TGG 0.430455 5.1:+7963457 None:intergenic
GAAGGAAATTGGTGCTAGAA+TGG 0.434419 5.1:-7962859 MS.gene20299:CDS
AACGAACTAAGCTCCCCTTA+TGG 0.445036 5.1:-7963251 MS.gene20299:CDS
GTAATGGTGTTTGAGGTTTG+AGG 0.447709 5.1:+7963431 None:intergenic
TTCTCTCGAATGACCGAAGC+CGG 0.449633 5.1:-7963185 MS.gene20299:CDS
TCACGGCGTTTGCATGGTGG+AGG 0.454306 5.1:-7962375 MS.gene20299:CDS
CAGTGACGATGGTGGTTAAA+CGG 0.455896 5.1:+7962907 None:intergenic
ATGTTAGGGCTTTGTTGAGG+AGG 0.458311 5.1:-7962314 MS.gene20299:CDS
TTCGGCCATGTTGCATGCAA+TGG 0.459991 5.1:-7963056 MS.gene20299:CDS
TTTGTTCTATGTCACCATAA+GGG 0.462505 5.1:+7963237 None:intergenic
CTATAGCTCGTGCTGTTTCG+AGG 0.464786 5.1:+7963318 None:intergenic
CGGTGGTGTGGGCCAGTGCA+TGG 0.470323 5.1:-7962224 MS.gene20299:CDS
TGAAATCAGTTGTTGGTGGA+GGG 0.471301 5.1:+7963378 None:intergenic
CATTCAGTAACAGTTGGTAA+TGG 0.475130 5.1:-7962699 MS.gene20299:CDS
CATGCACTGGCCCACACCAC+CGG 0.476107 5.1:+7962225 None:intergenic
GTGAAATCAGTTGTTGGTGG+AGG 0.476494 5.1:+7963377 None:intergenic
GGAAGTTTGTGATGATGTTA+GGG 0.478484 5.1:-7962328 MS.gene20299:CDS
AAACACTCGTTACTCGTCTT+CGG 0.480391 5.1:+7962492 None:intergenic
CACGGCGTTTGCATGGTGGA+GGG 0.480736 5.1:-7962374 MS.gene20299:CDS
GGTTCTGAAGGTTCTGGTAC+CGG 0.480940 5.1:+7963166 None:intergenic
ATGGTTTAGGGTTTACTTTG+AGG 0.492134 5.1:-7962532 MS.gene20299:CDS
GATGGATATGAATTTGTGAA+TGG 0.492688 5.1:-7962573 MS.gene20299:CDS
TCAAAGGTGAAATCAGTTGT+TGG 0.501730 5.1:+7963371 None:intergenic
TTGATGCTTGAAAGGGAAGC+TGG 0.502787 5.1:-7962471 MS.gene20299:CDS
ACGAGTGTTTGATGCTTGAA+AGG 0.503134 5.1:-7962479 MS.gene20299:CDS
TGTTTCGAGGAGGATGTTGT+TGG 0.505657 5.1:+7963331 None:intergenic
CCACTTTCCAGTTAATTCAA+AGG 0.510883 5.1:+7963355 None:intergenic
GAAATCAGTTGTTGGTGGAG+GGG 0.511614 5.1:+7963379 None:intergenic
GCATGGTGGAGGGTTTAATA+CGG 0.512491 5.1:-7962364 MS.gene20299:CDS
CCTTTGAATTAACTGGAAAG+TGG 0.516151 5.1:-7963355 MS.gene20299:CDS
TGATTTGGTGGCATGTGCTC+CGG 0.516469 5.1:-7962436 MS.gene20299:CDS
TTATCAAGGCCTCCATGCAC+TGG 0.517596 5.1:+7962212 None:intergenic
GGAAGGAACAACCGGTGGTG+TGG 0.520705 5.1:-7962236 MS.gene20299:CDS
GAGAGGGATAGTTGATTTGG+TGG 0.522738 5.1:-7962448 MS.gene20299:CDS
CTTGACGTGGAAGGAACAAC+CGG 0.523785 5.1:-7962244 MS.gene20299:CDS
AGGAAGGGTGGTCGATGATA+CGG 0.526878 5.1:-7962287 MS.gene20299:CDS
CTCCGTTCAATCGACTCTGC+CGG 0.527978 5.1:+7962417 None:intergenic
ATTCAACGTTATTCATCACT+CGG 0.529452 5.1:-7962802 MS.gene20299:CDS
GAAGGAACAACCGGTGGTGT+GGG 0.535300 5.1:-7962235 MS.gene20299:CDS
GTCGATTGAACGGAGAGAAA+CGG 0.535584 5.1:-7962409 MS.gene20299:CDS
TCAACCACTGTTACTATCCT+CGG 0.540676 5.1:+7962624 None:intergenic
TTGTTCTATGTCACCATAAG+GGG 0.542773 5.1:+7963238 None:intergenic
CTTGAAAGGGAAGCTGGGAG+AGG 0.543069 5.1:-7962465 MS.gene20299:CDS
CGGAATATTCTTGACGTGGA+AGG 0.543303 5.1:-7962253 MS.gene20299:CDS
CGAGTGTTTGATGCTTGAAA+GGG 0.545565 5.1:-7962478 MS.gene20299:CDS
GGAGAGAAACGGCGGTGAGA+TGG 0.549109 5.1:-7962398 MS.gene20299:CDS
TTAACCACCATCGTCACTGC+TGG 0.551969 5.1:-7962903 MS.gene20299:CDS
GATAGTAACAGTGGTTGAAG+AGG 0.553960 5.1:-7962619 MS.gene20299:CDS
TGCTGAAGCTAGGGTTCTGA+AGG 0.554762 5.1:+7963154 None:intergenic
GCTTTGCATTCAGTAACAGT+TGG 0.557029 5.1:-7962705 MS.gene20299:CDS
AGATGGTCACGGCGTTTGCA+TGG 0.559260 5.1:-7962381 MS.gene20299:CDS
CTTTGAATTAACTGGAAAGT+GGG 0.560664 5.1:-7963354 MS.gene20299:CDS
AATTCCAAGAAGTTAGTCCA+TGG 0.567956 5.1:-7963085 MS.gene20299:CDS
CAACTTAGAATCACCTTCGA+AGG 0.574160 5.1:+7963019 None:intergenic
ATGATACGGTGTAATGATGC+CGG 0.576413 5.1:-7962273 MS.gene20299:CDS
ACCACCATCGTCACTGCTGG+TGG 0.581472 5.1:-7962900 MS.gene20299:CDS
AGTAACAGTGGTTGAAGAGG+AGG 0.584206 5.1:-7962616 MS.gene20299:CDS
CTCCGGCAGAGTCGATTGAA+CGG 0.585476 5.1:-7962419 MS.gene20299:CDS
TGTTGAGGAGGTATAAGGAA+GGG 0.588341 5.1:-7962302 MS.gene20299:CDS
TTGTTGAGGAGGTATAAGGA+AGG 0.589974 5.1:-7962303 MS.gene20299:CDS
CGGAGTATCATCAGCACGAG+TGG 0.595472 5.1:+7962935 None:intergenic
GTAACAGTTGGTAATGGTAA+CGG 0.598007 5.1:-7962693 MS.gene20299:CDS
AAGGTGAAATCAGTTGTTGG+TGG 0.600375 5.1:+7963374 None:intergenic
TCATTCGAGAGAATAAGGCT+TGG 0.601622 5.1:+7963195 None:intergenic
TTGAAAGGGAAGCTGGGAGA+GGG 0.603420 5.1:-7962464 MS.gene20299:CDS
TGATGCTTGAAAGGGAAGCT+GGG 0.604533 5.1:-7962470 MS.gene20299:CDS
TTCAACGTTATTCATCACTC+GGG 0.607328 5.1:-7962801 MS.gene20299:CDS
ATGATGTTAGGGCTTTGTTG+AGG 0.607674 5.1:-7962317 MS.gene20299:CDS
CTCGTTTACAACAACTCATG+TGG 0.608017 5.1:-7963280 MS.gene20299:CDS
ACATGGCCGAAAGTAGTCCA+TGG 0.613056 5.1:+7963068 None:intergenic
GTTTGGAAGAAGATGATGAA+AGG 0.615269 5.1:+7963474 None:intergenic
AAGCTATCAACGCGTCGCGA+CGG 0.617261 5.1:+7962664 None:intergenic
TGGCGCCATTGCATGCAACA+TGG 0.621243 5.1:+7963051 None:intergenic
TGAGGAGGTATAAGGAAGGG+TGG 0.622191 5.1:-7962299 MS.gene20299:CDS
TAGCTTCGTTGATCGCGTTG+CGG 0.632402 5.1:-7962647 MS.gene20299:CDS
TGGTCACGGCGTTTGCATGG+TGG 0.640251 5.1:-7962378 MS.gene20299:CDS
AAACGGCGGTGAGATGGTCA+CGG 0.645102 5.1:-7962392 MS.gene20299:CDS
TAATACGGTGGCGTTTAGTG+AGG 0.648084 5.1:-7962349 MS.gene20299:CDS
AGAACCACCAGCAGTGACGA+TGG 0.652328 5.1:+7962896 None:intergenic
TGGTGGAGGGTTTAATACGG+TGG 0.662791 5.1:-7962361 MS.gene20299:CDS
ATGCCGGAATATTCTTGACG+TGG 0.670161 5.1:-7962257 MS.gene20299:CDS
GTAACACTTATTGCACTCAG+TGG 0.674128 5.1:-7962980 MS.gene20299:CDS
ATGGTGGTTAAACGGAGGTG+CGG 0.674756 5.1:+7962915 None:intergenic
TAGCTCGTGCTGTTTCGAGG+AGG 0.677191 5.1:+7963321 None:intergenic
TGGTGTGGGCCAGTGCATGG+AGG 0.695562 5.1:-7962221 MS.gene20299:CDS
GACGTGGAAGGAACAACCGG+TGG 0.699506 5.1:-7962241 MS.gene20299:CDS
GCGGCCGAGGATAGTAACAG+TGG 0.704672 5.1:-7962628 MS.gene20299:CDS
ACCACCAGCAGTGACGATGG+TGG 0.722652 5.1:+7962899 None:intergenic
GATTGAACGGAGAGAAACGG+CGG 0.736491 5.1:-7962406 MS.gene20299:CDS
TGACGATGGTGGTTAAACGG+AGG 0.736521 5.1:+7962910 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTTTCTGATTTAAATTTTT+TGG - chr5.1:7962928-7962947 MS.gene20299:CDS 15.0%
!! TGAATAACGTTGAATTTAAA+TGG + chr5.1:7962896-7962915 None:intergenic 20.0%
!!! GATTTAAATTTTGGTAATGA+TGG - chr5.1:7963112-7963131 MS.gene20299:CDS 20.0%
! CAAAAAGTTATGAAGGAAAT+TGG - chr5.1:7962833-7962852 MS.gene20299:CDS 25.0%
! TTCTGTTCAAAAAGTTATGA+AGG - chr5.1:7962826-7962845 MS.gene20299:CDS 25.0%
ACTATAAGGATAGTAGTGTT+TGG + chr5.1:7962249-7962268 None:intergenic 30.0%
ATTCAACGTTATTCATCACT+CGG - chr5.1:7962901-7962920 MS.gene20299:CDS 30.0%
CTTTGAATTAACTGGAAAGT+GGG - chr5.1:7962349-7962368 MS.gene20299:CDS 30.0%
GAAAAATTCGCTAGACTTAT+GGG - chr5.1:7962866-7962885 MS.gene20299:CDS 30.0%
GATGGATATGAATTTGTGAA+TGG - chr5.1:7963130-7963149 MS.gene20299:CDS 30.0%
GATTTCACCTTTGAATTAAC+TGG - chr5.1:7962341-7962360 MS.gene20299:CDS 30.0%
TTTGTTCTATGTCACCATAA+GGG + chr5.1:7962469-7962488 None:intergenic 30.0%
! GACTAACTTCTTGGAATTTT+AGG + chr5.1:7962616-7962635 None:intergenic 30.0%
! TTTTGTTCTATGTCACCATA+AGG + chr5.1:7962470-7962489 None:intergenic 30.0%
AATTCCAAGAAGTTAGTCCA+TGG - chr5.1:7962618-7962637 MS.gene20299:CDS 35.0%
CCACTTTCCAGTTAATTCAA+AGG + chr5.1:7962351-7962370 None:intergenic 35.0%
CCTTTGAATTAACTGGAAAG+TGG - chr5.1:7962348-7962367 MS.gene20299:CDS 35.0%
GGAAAAATTCGCTAGACTTA+TGG - chr5.1:7962865-7962884 MS.gene20299:CDS 35.0%
GTTTGGAAGAAGATGATGAA+AGG + chr5.1:7962232-7962251 None:intergenic 35.0%
GTTTGTAGTAATGGTGTTTG+AGG + chr5.1:7962282-7962301 None:intergenic 35.0%
TCAAAGGTGAAATCAGTTGT+TGG + chr5.1:7962335-7962354 None:intergenic 35.0%
TTCAACGTTATTCATCACTC+GGG - chr5.1:7962902-7962921 MS.gene20299:CDS 35.0%
TTGTTCTATGTCACCATAAG+GGG + chr5.1:7962468-7962487 None:intergenic 35.0%
! AGAGTGTTTGAGATGGTTTA+GGG - chr5.1:7963159-7963178 MS.gene20299:CDS 35.0%
! AGGAAGTTTGTGATGATGTT+AGG - chr5.1:7963374-7963393 MS.gene20299:CDS 35.0%
! ATGGTTTAGGGTTTACTTTG+AGG - chr5.1:7963171-7963190 MS.gene20299:CDS 35.0%
! GGAAGTTTGTGATGATGTTA+GGG - chr5.1:7963375-7963394 MS.gene20299:CDS 35.0%
! TTTTTCTGATGCTGAAGCTA+GGG + chr5.1:7962561-7962580 None:intergenic 35.0%
!! AAGAGTGTTTGAGATGGTTT+AGG - chr5.1:7963158-7963177 MS.gene20299:CDS 35.0%
!! CATTCAGTAACAGTTGGTAA+TGG - chr5.1:7963004-7963023 MS.gene20299:CDS 35.0%
!! GAGGAGGCTGATTTAAATTT+TGG - chr5.1:7963103-7963122 MS.gene20299:CDS 35.0%
!! GTAACAGTTGGTAATGGTAA+CGG - chr5.1:7963010-7963029 MS.gene20299:CDS 35.0%
!!! GTTTTGAAGAGTGTTTGAGA+TGG - chr5.1:7963152-7963171 MS.gene20299:CDS 35.0%
!!! TTTTTTCTGATGCTGAAGCT+AGG + chr5.1:7962562-7962581 None:intergenic 35.0%
AAACACTCGTTACTCGTCTT+CGG + chr5.1:7963214-7963233 None:intergenic 40.0%
AAGGTGAAATCAGTTGTTGG+TGG + chr5.1:7962332-7962351 None:intergenic 40.0%
ACGAGTGTTTGATGCTTGAA+AGG - chr5.1:7963224-7963243 MS.gene20299:CDS 40.0%
ATGATACGGTGTAATGATGC+CGG - chr5.1:7963430-7963449 MS.gene20299:CDS 40.0%
CAACTTAGAATCACCTTCGA+AGG + chr5.1:7962687-7962706 None:intergenic 40.0%
CGAGTGTTTGATGCTTGAAA+GGG - chr5.1:7963225-7963244 MS.gene20299:CDS 40.0%
CTCGTTTACAACAACTCATG+TGG - chr5.1:7962423-7962442 MS.gene20299:CDS 40.0%
CTTCCACGTCAAGAATATTC+CGG + chr5.1:7963452-7963471 None:intergenic 40.0%
GATAGTAACAGTGGTTGAAG+AGG - chr5.1:7963084-7963103 MS.gene20299:CDS 40.0%
GCTTTGCATTCAGTAACAGT+TGG - chr5.1:7962998-7963017 MS.gene20299:CDS 40.0%
GTAACACTTATTGCACTCAG+TGG - chr5.1:7962723-7962742 MS.gene20299:CDS 40.0%
TCAACCACTGTTACTATCCT+CGG + chr5.1:7963082-7963101 None:intergenic 40.0%
TCATTCGAGAGAATAAGGCT+TGG + chr5.1:7962511-7962530 None:intergenic 40.0%
TTCGGTCATTCGAGAGAATA+AGG + chr5.1:7962516-7962535 None:intergenic 40.0%
! ACCTTCGAAGGCTTCTAAAA+TGG + chr5.1:7962675-7962694 None:intergenic 40.0%
! GAGGTTTGAGGTTGACTATA+AGG + chr5.1:7962263-7962282 None:intergenic 40.0%
! GTAATGGTGTTTGAGGTTTG+AGG + chr5.1:7962275-7962294 None:intergenic 40.0%
! GTTAGTCCATGGACTACTTT+CGG - chr5.1:7962629-7962648 MS.gene20299:CDS 40.0%
! TAGTCCATGGACTAACTTCT+TGG + chr5.1:7962625-7962644 None:intergenic 40.0%
! TGAAATCAGTTGTTGGTGGA+GGG + chr5.1:7962328-7962347 None:intergenic 40.0%
! TGTTGAGGAGGTATAAGGAA+GGG - chr5.1:7963401-7963420 MS.gene20299:CDS 40.0%
! TTGTTGAGGAGGTATAAGGA+AGG - chr5.1:7963400-7963419 MS.gene20299:CDS 40.0%
!! ATGATGTTAGGGCTTTGTTG+AGG - chr5.1:7963386-7963405 MS.gene20299:CDS 40.0%
!! GAAGGAAATTGGTGCTAGAA+TGG - chr5.1:7962844-7962863 MS.gene20299:CDS 40.0%
AACGAACTAAGCTCCCCTTA+TGG - chr5.1:7962452-7962471 MS.gene20299:CDS 45.0%
AGTAACAGTGGTTGAAGAGG+AGG - chr5.1:7963087-7963106 MS.gene20299:CDS 45.0%
ATGCCGGAATATTCTTGACG+TGG - chr5.1:7963446-7963465 MS.gene20299:CDS 45.0%
CGGAATATTCTTGACGTGGA+AGG - chr5.1:7963450-7963469 MS.gene20299:CDS 45.0%
GTCGATTGAACGGAGAGAAA+CGG - chr5.1:7963294-7963313 MS.gene20299:CDS 45.0%
TAATACGGTGGCGTTTAGTG+AGG - chr5.1:7963354-7963373 MS.gene20299:CDS 45.0%
TGATGCTTGAAAGGGAAGCT+GGG - chr5.1:7963233-7963252 MS.gene20299:CDS 45.0%
TGGGAGAGGGATAGTTGATT+TGG - chr5.1:7963252-7963271 MS.gene20299:CDS 45.0%
TGTTTCGAGGAGGATGTTGT+TGG + chr5.1:7962375-7962394 None:intergenic 45.0%
TTGATGCTTGAAAGGGAAGC+TGG - chr5.1:7963232-7963251 MS.gene20299:CDS 45.0%
! AAATCAGTTGTTGGTGGAGG+GGG + chr5.1:7962326-7962345 None:intergenic 45.0%
! CAGTGACGATGGTGGTTAAA+CGG + chr5.1:7962799-7962818 None:intergenic 45.0%
! GAAATCAGTTGTTGGTGGAG+GGG + chr5.1:7962327-7962346 None:intergenic 45.0%
! GCATGGTGGAGGGTTTAATA+CGG - chr5.1:7963339-7963358 MS.gene20299:CDS 45.0%
! GTGAAATCAGTTGTTGGTGG+AGG + chr5.1:7962329-7962348 None:intergenic 45.0%
!! ATGTTAGGGCTTTGTTGAGG+AGG - chr5.1:7963389-7963408 MS.gene20299:CDS 45.0%
!! GAGAGGGATAGTTGATTTGG+TGG - chr5.1:7963255-7963274 MS.gene20299:CDS 45.0%
!! GCCATTTTAGAAGCCTTCGA+AGG - chr5.1:7962671-7962690 MS.gene20299:CDS 45.0%
!! GGCTTTGTTGAGGAGGTATA+AGG - chr5.1:7963396-7963415 MS.gene20299:CDS 45.0%
!! TGTGGGGGTGTTTGTAGTAA+TGG + chr5.1:7962291-7962310 None:intergenic 45.0%
ACATGGCCGAAAGTAGTCCA+TGG + chr5.1:7962638-7962657 None:intergenic 50.0%
AGGAAGGGTGGTCGATGATA+CGG - chr5.1:7963416-7963435 MS.gene20299:CDS 50.0%
ATGGTGGTTAAACGGAGGTG+CGG + chr5.1:7962791-7962810 None:intergenic 50.0%
CTTGACGTGGAAGGAACAAC+CGG - chr5.1:7963459-7963478 MS.gene20299:CDS 50.0%
GATTGAACGGAGAGAAACGG+CGG - chr5.1:7963297-7963316 MS.gene20299:CDS 50.0%
TAGCTTCGTTGATCGCGTTG+CGG - chr5.1:7963056-7963075 MS.gene20299:CDS 50.0%
TGGTGGAGGGTTTAATACGG+TGG - chr5.1:7963342-7963361 MS.gene20299:CDS 50.0%
TTAACCACCATCGTCACTGC+TGG - chr5.1:7962800-7962819 MS.gene20299:CDS 50.0%
TTCGGCCATGTTGCATGCAA+TGG - chr5.1:7962647-7962666 MS.gene20299:CDS 50.0%
TTCTCTCGAATGACCGAAGC+CGG - chr5.1:7962518-7962537 MS.gene20299:CDS 50.0%
TTGAAAGGGAAGCTGGGAGA+GGG - chr5.1:7963239-7963258 MS.gene20299:CDS 50.0%
! GCCAACGCTTCAAAAAGCGT+TGG + chr5.1:7962749-7962768 None:intergenic 50.0%
! TGACGATGGTGGTTAAACGG+AGG + chr5.1:7962796-7962815 None:intergenic 50.0%
! TGAGGAGGTATAAGGAAGGG+TGG - chr5.1:7963404-7963423 MS.gene20299:CDS 50.0%
! TGCTGAAGCTAGGGTTCTGA+AGG + chr5.1:7962552-7962571 None:intergenic 50.0%
!! AGCTAGGGTTCTGAAGGTTC+TGG + chr5.1:7962546-7962565 None:intergenic 50.0%
!! CTATAGCTCGTGCTGTTTCG+AGG + chr5.1:7962388-7962407 None:intergenic 50.0%
!! GGTTCTGAAGGTTCTGGTAC+CGG + chr5.1:7962540-7962559 None:intergenic 50.0%
!! TGATTTGGTGGCATGTGCTC+CGG - chr5.1:7963267-7963286 MS.gene20299:CDS 50.0%
!!! GCCAACGCTTTTTGAAGCGT+TGG - chr5.1:7962745-7962764 MS.gene20299:CDS 50.0%
AAACGGCGGTGAGATGGTCA+CGG - chr5.1:7963311-7963330 MS.gene20299:CDS 55.0%
AAGCTATCAACGCGTCGCGA+CGG + chr5.1:7963042-7963061 None:intergenic 55.0%
AGAACCACCAGCAGTGACGA+TGG + chr5.1:7962810-7962829 None:intergenic 55.0%
AGATGGTCACGGCGTTTGCA+TGG - chr5.1:7963322-7963341 MS.gene20299:CDS 55.0%
CTCCGGCAGAGTCGATTGAA+CGG - chr5.1:7963284-7963303 MS.gene20299:CDS 55.0%
CTTGAAAGGGAAGCTGGGAG+AGG - chr5.1:7963238-7963257 MS.gene20299:CDS 55.0%
GAAGGAACAACCGGTGGTGT+GGG - chr5.1:7963468-7963487 MS.gene20299:CDS 55.0%
TGGCGCCATTGCATGCAACA+TGG + chr5.1:7962655-7962674 None:intergenic 55.0%
! CGGAGTATCATCAGCACGAG+TGG + chr5.1:7962771-7962790 None:intergenic 55.0%
! CTCCGTTCAATCGACTCTGC+CGG + chr5.1:7963289-7963308 None:intergenic 55.0%
!! GAAGGTTCTGGTACCGGCTT+CGG + chr5.1:7962534-7962553 None:intergenic 55.0%
!! TAGCTCGTGCTGTTTCGAGG+AGG + chr5.1:7962385-7962404 None:intergenic 55.0%
ACCACCAGCAGTGACGATGG+TGG + chr5.1:7962807-7962826 None:intergenic 60.0%
ACCACCATCGTCACTGCTGG+TGG - chr5.1:7962803-7962822 MS.gene20299:CDS 60.0%
GACGTGGAAGGAACAACCGG+TGG - chr5.1:7963462-7963481 MS.gene20299:CDS 60.0%
GCGGCCGAGGATAGTAACAG+TGG - chr5.1:7963075-7963094 MS.gene20299:CDS 60.0%
GGAAGGAACAACCGGTGGTG+TGG - chr5.1:7963467-7963486 MS.gene20299:CDS 60.0%
GGAGAGAAACGGCGGTGAGA+TGG - chr5.1:7963305-7963324 MS.gene20299:CDS 60.0%
TCACGGCGTTTGCATGGTGG+AGG - chr5.1:7963328-7963347 MS.gene20299:CDS 60.0%
TGGTCACGGCGTTTGCATGG+TGG - chr5.1:7963325-7963344 MS.gene20299:CDS 60.0%
! AGGGGGTGTGAGTGATGGTG+TGG + chr5.1:7962309-7962328 None:intergenic 60.0%
! CACGGCGTTTGCATGGTGGA+GGG - chr5.1:7963329-7963348 MS.gene20299:CDS 60.0%
! GGGGGTGTGAGTGATGGTGT+GGG + chr5.1:7962308-7962327 None:intergenic 60.0%
! GGGGTGTGAGTGATGGTGTG+GGG + chr5.1:7962307-7962326 None:intergenic 60.0%
! GGGTGTGAGTGATGGTGTGG+GGG + chr5.1:7962306-7962325 None:intergenic 60.0%
TGGTGTGGGCCAGTGCATGG+AGG - chr5.1:7963482-7963501 MS.gene20299:CDS 65.0%
! CATGCACTGGCCCACACCAC+CGG + chr5.1:7963481-7963500 None:intergenic 65.0%
! GGTGGAGGGGGTGTGAGTGA+TGG + chr5.1:7962314-7962333 None:intergenic 65.0%
CGGTGGTGTGGGCCAGTGCA+TGG - chr5.1:7963479-7963498 MS.gene20299:CDS 70.0%
CGTTGATCGCGTTGCGGCCG+AGG - chr5.1:7963062-7963081 MS.gene20299:CDS 70.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 7962215 7963510 7962215 ID=MS.gene20299
chr5.1 mRNA 7962215 7963510 7962215 ID=MS.gene20299.t1;Parent=MS.gene20299
chr5.1 exon 7962215 7963510 7962215 ID=MS.gene20299.t1.exon1;Parent=MS.gene20299.t1
chr5.1 CDS 7962215 7963510 7962215 ID=cds.MS.gene20299.t1;Parent=MS.gene20299.t1
Gene Sequence

>MS.gene20299

ATGGATGAAGAAGACCTTTCATCATCTTCTTCCAAACACTACTATCCTTATAGTCAACCTCAAACCTCAAACACCATTACTACAAACACCCCCACACCATCACTCACACCCCCTCCACCAACAACTGATTTCACCTTTGAATTAACTGGAAAGTGGGCCAACAACATCCTCCTCGAAACAGCACGAGCTATAGCAGAAAAAAACAGCACTCGTTTACAACAACTCATGTGGATGCTCAACGAACTAAGCTCCCCTTATGGTGACATAGAACAAAAACTAGCTGCATATTTTCTCCAAGCCTTATTCTCTCGAATGACCGAAGCCGGTACCAGAACCTTCAGAACCCTAGCTTCAGCATCAGAAAAAACTTGCTCATTCGAATCAACACGAAAAACAGTCCTAAAATTCCAAGAAGTTAGTCCATGGACTACTTTCGGCCATGTTGCATGCAATGGCGCCATTTTAGAAGCCTTCGAAGGTGATTCTAAGTTGCACATAATTGATATTAGTAACACTTATTGCACTCAGTGGCCAACGCTTTTTGAAGCGTTGGCCACTCGTGCTGATGATACTCCGCACCTCCGTTTAACCACCATCGTCACTGCTGGTGGTTCTGTTCAAAAAGTTATGAAGGAAATTGGTGCTAGAATGGAAAAATTCGCTAGACTTATGGGAGTTCCATTTAAATTCAACGTTATTCATCACTCGGGCGATCTTTCTGATTTAAATTTTTTGGATTTAGATATTAAAGAAGATGAAGCTTTAGCTGTTAATTGTGTTAATGCTTTGCATTCAGTAACAGTTGGTAATGGTAACGGTAATAACCGTCGCGACGCGTTGATAGCTTCGTTGATCGCGTTGCGGCCGAGGATAGTAACAGTGGTTGAAGAGGAGGCTGATTTAAATTTTGGTAATGATGGATATGAATTTGTGAATGGTTTTGAAGAGTGTTTGAGATGGTTTAGGGTTTACTTTGAGGCATTAGAAGAGAGTTTTCCGAAGACGAGTAACGAGTGTTTGATGCTTGAAAGGGAAGCTGGGAGAGGGATAGTTGATTTGGTGGCATGTGCTCCGGCAGAGTCGATTGAACGGAGAGAAACGGCGGTGAGATGGTCACGGCGTTTGCATGGTGGAGGGTTTAATACGGTGGCGTTTAGTGAGGAAGTTTGTGATGATGTTAGGGCTTTGTTGAGGAGGTATAAGGAAGGGTGGTCGATGATACGGTGTAATGATGCCGGAATATTCTTGACGTGGAAGGAACAACCGGTGGTGTGGGCCAGTGCATGGAGGCCTTGA
Protein sequence

>MS.gene20299.t1

MDEEDLSSSSSKHYYPYSQPQTSNTITTNTPTPSLTPPPPTTDFTFELTGKWANNILLETARAIAEKNSTRLQQLMWMLNELSSPYGDIEQKLAAYFLQALFSRMTEAGTRTFRTLASASEKTCSFESTRKTVLKFQEVSPWTTFGHVACNGAILEAFEGDSKLHIIDISNTYCTQWPTLFEALATRADDTPHLRLTTIVTAGGSVQKVMKEIGARMEKFARLMGVPFKFNVIHHSGDLSDLNFLDLDIKEDEALAVNCVNALHSVTVGNGNGNNRRDALIASLIALRPRIVTVVEEEADLNFGNDGYEFVNGFEECLRWFRVYFEALEESFPKTSNECLMLEREAGRGIVDLVACAPAESIERRETAVRWSRRLHGGGFNTVAFSEEVCDDVRALLRRYKEGWSMIRCNDAGIFLTWKEQPVVWASAWRP