AlfalfaGEDB | MS.gene20468

M. sativa cultivar XinJiangDaYe / MS.gene20468

KEGG: K14400
GO: GO:0003674 GO:0003676 GO:0005488 GO:0097159 GO:1901363
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20468.t1	XP_013450939.1	64.8	546	97	13	2	474	290	813	3.50E-177	631.3

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20468.t1	Q0WPF2	39.5	509	215	13	33	473	306	789	1.8e-81	304.7

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20468.t1	A0A072U5F2	64.8	546	97	13	2	474	152	675	2.6e-177	631.3

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene20468.t1	TF	C2H2

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20468.t1	MTR_6g011450	66.355	535	80	12	15	474	304	813	0.0	630
MS.gene20468.t1	MTR_6g011450	66.355	535	80	12	15	474	166	675	0.0	628
MS.gene20468.t1	MTR_4g073340	59.924	529	103	11	6	474	15	494	0.0	535
MS.gene20468.t1	MTR_6g011470	72.692	260	20	2	216	474	1	210	7.97e-131	377
MS.gene20468.t1	MTR_1g061130	32.812	256	127	6	262	474	755	1008	2.07e-37	146
MS.gene20468.t1	MTR_1g061130	32.812	256	127	6	262	474	755	1008	2.33e-37	146
MS.gene20468.t1	MTR_7g103840	30.337	267	143	6	249	474	751	1015	1.62e-34	138
MS.gene20468.t1	MTR_7g103840	30.337	267	143	6	249	474	749	1013	1.79e-34	137

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene20468.t1	AT4G04885	63.801	221	58	2	253	473	591	789	4.16e-95	305
MS.gene20468.t1	AT1G66500	36.176	340	172	15	159	472	66	386	9.22e-51	178
MS.gene20468.t1	AT5G43620	36.170	282	132	10	215	472	123	380	1.67e-49	174
MS.gene20468.t1	AT2G36480	33.333	180	107	4	293	472	776	942	1.13e-25	111
MS.gene20468.t1	AT2G36480	33.333	180	107	4	293	472	776	942	1.13e-25	111

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Find 139 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTGATGAAGAATTTGT+TGG	0.474608	6.3:-6124174	None:intergenic
AAAGAAAGAAGATGAAGAGT+TGG	0.403383	6.3:+6125463	MS.gene20468:CDS
AAAGGAAGAAGATGCTTGCC+AGG	0.480340	6.3:+6124142	None:intergenic
AAAGTGAATTGCGACGCTGC+TGG	0.570896	6.3:-6124738	None:intergenic
AAAGTTGCTGGCGAAACTGG+TGG	0.614136	6.3:-6124462	None:intergenic
AAATTAGAATATCCGACAGT+TGG	0.551878	6.3:-6124924	None:intergenic
AACAGGTCGTGCATTTGGCA+GGG	0.545446	6.3:-6124775	None:intergenic
AAGAGTTGTTTCCGATTCTT+TGG	0.325210	6.3:-6124818	None:intergenic
AATACTACCACTGTGAGTCA+TGG	0.609522	6.3:+6124579	MS.gene20468:CDS
AATATCCGACAGTTGGCTGT+TGG	0.554695	6.3:-6124917	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGAAAGAAGATGAAGAGT+TGG	+	chr6.3:6125463-6125482	MS.gene20468:CDS	30.0%
AAATTAGAATATCCGACAGT+TGG	-	chr6.3:6124927-6124946	None:intergenic	30.0%
CAAAATACATGTGCATTATG+TGG	+	chr6.3:6125506-6125525	MS.gene20468:CDS	30.0%
GAATATTTGGATCAAACTCA+AGG	-	chr6.3:6125181-6125200	None:intergenic	30.0%
GGTACGCAATTAAACAATAA+TGG	-	chr6.3:6125120-6125139	None:intergenic	30.0%
GTTTGATCCAAATATTCTCA+AGG	+	chr6.3:6125184-6125203	MS.gene20468:CDS	30.0%
TCTGCATTTAGCATGAATAA+CGG	-	chr6.3:6125648-6125667	None:intergenic	30.0%
AGCTAATGAGATAACACCTT+GGG	-	chr6.3:6124967-6124986	None:intergenic	35.0%
CGAAAAGACAAGAAACTTCA+TGG	+	chr6.3:6124298-6124317	MS.gene20468:CDS	35.0%
CTGCATTTAGCATGAATAAC+GGG	-	chr6.3:6125647-6125666	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr6.3	gene	6124153	6125673	6124153	ID=MS.gene20468
chr6.3	mRNA	6124153	6125673	6124153	ID=MS.gene20468.t1;Parent=MS.gene20468
chr6.3	exon	6124153	6125070	6124153	ID=MS.gene20468.t1.exon1;Parent=MS.gene20468.t1
chr6.3	CDS	6124153	6125070	6124153	ID=cds.MS.gene20468.t1;Parent=MS.gene20468.t1
chr6.3	exon	6125167	6125673	6125167	ID=MS.gene20468.t1.exon2;Parent=MS.gene20468.t1
chr6.3	CDS	6125167	6125673	6125167	ID=cds.MS.gene20468.t1;Parent=MS.gene20468.t1

Gene Sequence

>MS.gene20468

ATGCTTGCCAGGAGAGAAACACCAACAAATTCTTCATCAGTTTTCTCACACTTTCCGCGAAAAAGATGTTTTGATGAGTTCAGCTATCGCAGCAACGGACAACCTCGTCAAAATCCAATAGCTCTAATTGATGCCTATGGTAGTGCGAAAAGACAAGAAACTTCATGGAAGAATACCGAAGAGGAGGAGTTCAATTGGGAAGATATGATTCCGACACCAGCAACTGGGAAGCCCGCTACTATTGCAGCAAATGCAACTTCTTCAGCGTCAAGGGTGTTTCCTGGTCTCAATTCTAATACTGAGTACCATCCACCAGTTTCGCCAGCAACTTTTGAAACGAGGCCCTCCGTAAATGTGCATGCTCCACGTCCTCCTATATATCCATCGAAGAATCCTGTTAGGAATCCATTCGAATCAATAAATGCTAATACTACCACTGTGAGTCATGGTCTAATTAATAGACCTATTCCTGTGCATGAACATGGTGTCGAGAACAATGGTATCAGTAAAAGAAACCTGCATCAGTTGCCTAACTCGCCAACCAGTGGACAAACACCGCCTTTCCAGTTTTTTCCATCTCAGAATCCAGCAGCGTCGCAATTCACTTTGCCAAGACATGCCCCCCTGCCAAATGCACGACCTGTTATGCCACTTCCTATGCCAGACCAAAGAATCGGAAACAACTCTTTCCGACCTTTACCTCCAGCTGGCCATGCTCTACCCCAGATTCTACCTCATCCGAATCCTGGTCCTAATGTGTCAAGCCAACAGCCAACTGTCGGATATTCTAATTTGATTAGTTCACTCGTGGCCCAAGGTGTTATCTCATTAGCTAATCAAGCCCCTGCGGAGGTAACTAAATCGGCTAATCAAACCCCTGCGGAGGTAACTAAATCGGCTAATCAAGCCCCTGCGGAGGTAACTAAATCAATCCTTAGTTCAATTTTTATATCACTTTTTCCTTCCATTATTGTTTAATTGCGTACCAATTTTGTTATTATATTATGTTTGTAGGATTTTATTGGCCTTGAGTTTGATCCAAATATTCTCAAGGTTCGTCATAAATCTGTAATCCGTGGCTTATATGGTGATTTTCCTAGACAGTGCACAACTTGTGGCCTCCGATTCAAATCCAAAGATGAGCACAGCAGTCACATGGATTGGCATGTAACTAAGAATCGTATGTCTAAAAACAATAAGCAGAAACCCTCTCGCAAGTGGTTTGTGAGTGAGACGATGTGGCTTAAAGGTGCCGAGGCATTGGGAGTCAAGTCAGCTCCAGGTTTTTTGCCAACCGAGACCACTGAAGAAAAGAAAGAAGATGAAGAGTTGGCTGTTCCGGCCGACGAGGACCAAAATACATGTGCATTATGTGGAGAACCTTTTGAGGAGTTTTACAGTGATGAAACGGAGGAGTGGATGTATAGAGGAGCTGTATACCTTAATGCGCCTAATGGAATAACAACAGGCATAGACAGGTCGCAGTTTGGCCCCGTTATTCATGCTAAATGCAGATCTTAA

Protein sequence

>MS.gene20468.t1

MLARRETPTNSSSVFSHFPRKRCFDEFSYRSNGQPRQNPIALIDAYGSAKRQETSWKNTEEEEFNWEDMIPTPATGKPATIAANATSSASRVFPGLNSNTEYHPPVSPATFETRPSVNVHAPRPPIYPSKNPVRNPFESINANTTTVSHGLINRPIPVHEHGVENNGISKRNLHQLPNSPTSGQTPPFQFFPSQNPAASQFTLPRHAPLPNARPVMPLPMPDQRIGNNSFRPLPPAGHALPQILPHPNPGPNVSSQQPTVGYSNLISSLVAQGVISLANQAPAEVTKSANQTPAEVTKSANQAPAEDFIGLEFDPNILKVRHKSVIRGLYGDFPRQCTTCGLRFKSKDEHSSHMDWHVTKNRMSKNNKQKPSRKWFVSETMWLKGAEALGVKSAPGFLPTETTEEKKEDEELAVPADEDQNTCALCGEPFEEFYSDETEEWMYRGAVYLNAPNGITTGIDRSQFGPVIHAKCRS