AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20469

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20469.t1 MTR_6g011450 66.542 535 79 12 15 474 304 813 0.0 632
MS.gene20469.t1 MTR_6g011450 66.542 535 79 12 15 474 166 675 0.0 630
MS.gene20469.t1 MTR_4g073340 60.113 529 102 11 6 474 15 494 0.0 536
MS.gene20469.t1 MTR_6g011470 73.077 260 19 2 216 474 1 210 3.64e-131 378
MS.gene20469.t1 MTR_1g061130 32.812 256 127 6 262 474 755 1008 1.88e-37 146
MS.gene20469.t1 MTR_1g061130 32.812 256 127 6 262 474 755 1008 2.14e-37 146
MS.gene20469.t1 MTR_7g103840 30.337 267 143 6 249 474 751 1015 1.21e-34 138
MS.gene20469.t1 MTR_7g103840 30.337 267 143 6 249 474 749 1013 1.25e-34 138
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20469.t1 AT4G04885 64.253 221 57 2 253 473 591 789 1.28e-95 306
MS.gene20469.t1 AT1G66500 36.176 340 172 15 159 472 66 386 1.10e-50 178
MS.gene20469.t1 AT5G43620 36.170 282 132 10 215 472 123 380 2.21e-49 174
MS.gene20469.t1 AT2G36480 33.333 180 107 4 293 472 776 942 1.44e-25 110
MS.gene20469.t1 AT2G36480 33.333 180 107 4 293 472 776 942 1.44e-25 110

Find 139 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACGAACCTTGAGAATATT+TGG 0.171658 6.3:-6112254 None:intergenic
CACATAATGCACATGTATTT+TGG 0.204278 6.3:-6112568 None:intergenic
GTGACTGCTGTGCTCATCTT+TGG 0.248435 6.3:-6112348 None:intergenic
TAGGCATCAATTAGAGCTAT+TGG 0.294593 6.3:-6111330 None:intergenic
ATGCCTGTTGTTATTCCATT+AGG 0.320007 6.3:-6112665 None:intergenic
TGTCCACTGGTTGGCGAGTT+AGG 0.324685 6.3:-6111744 None:intergenic
AAGAGTTGTTTCCGATTCTT+TGG 0.325210 6.3:-6111881 None:intergenic
TCAGCGTCAAGGGTGTTTCC+TGG 0.325987 6.3:+6111477 MS.gene20469:CDS
TCAAAAGTTGCTGGCGAAAC+TGG 0.326718 6.3:-6111528 None:intergenic
TCTGTAATCCGTGGCTTATA+TGG 0.327106 6.3:+6112281 MS.gene20469:CDS
GATTCGAATGGATTCCTAAC+AGG 0.333615 6.3:-6111609 None:intergenic
AGATGAAGAGTTGGCTGTTC+CGG 0.339955 6.3:+6112535 MS.gene20469:CDS
TTATTCCATTAGGCGCATTA+AGG 0.358655 6.3:-6112655 None:intergenic
CGAAAAGACAAGAAACTTCA+TGG 0.371299 6.3:+6111361 MS.gene20469:CDS
GATTCGGATGAGGTAGAATC+TGG 0.392316 6.3:-6111938 None:intergenic
CTGCATTTAGCATGAATAAC+GGG 0.394865 6.3:-6112707 None:intergenic
GGCTGTTGGCTTGACACATT+AGG 0.397611 6.3:-6111966 None:intergenic
ATGATTCCGACACCAGCAAC+TGG 0.398413 6.3:+6111420 MS.gene20469:CDS
GGATTCTTCGATGGATATAT+AGG 0.398722 6.3:-6111588 None:intergenic
GGCATAACAGGTCGTGCATT+TGG 0.398786 6.3:-6111843 None:intergenic
GGCATAGGAAGTGGCATAAC+AGG 0.399981 6.3:-6111855 None:intergenic
AAAGAAAGAAGATGAAGAGT+TGG 0.403383 6.3:+6112526 MS.gene20469:CDS
ACGTGGAGCATGCACATTTA+CGG 0.404948 6.3:-6111562 None:intergenic
GTAGCGGGCTTCCCAGTTGC+TGG 0.407709 6.3:-6111432 None:intergenic
CCGAAGAGGAGGAGTTCAAT+TGG 0.408130 6.3:+6111391 MS.gene20469:CDS
TAACAGGTCGTGCATTTGGC+AGG 0.413669 6.3:-6111839 None:intergenic
CCATGTTCATGCACAGGAAT+AGG 0.417748 6.3:-6111678 None:intergenic
GCTTAAAGGTGCCGAGGCAT+TGG 0.418088 6.3:+6112457 MS.gene20469:CDS
TTGTTTCCGATTCTTTGGTC+TGG 0.419863 6.3:-6111876 None:intergenic
ATTTGTTGGTGTTTCTCTCC+TGG 0.439045 6.3:-6111223 None:intergenic
AGTGAGACGATGTGGCTTAA+AGG 0.439793 6.3:+6112443 MS.gene20469:CDS
CCCTGCGGAGGTAACTAAAT+CGG 0.441235 6.3:+6112091 MS.gene20469:CDS
GTATACCTTAATGCGCCTAA+TGG 0.441302 6.3:+6112650 MS.gene20469:CDS
CCAATTGAACTCCTCCTCTT+CGG 0.441961 6.3:-6111391 None:intergenic
GCTCATCTTTGGATTTGAAT+CGG 0.447487 6.3:-6112337 None:intergenic
CTTAAAGGTGCCGAGGCATT+GGG 0.453885 6.3:+6112458 MS.gene20469:CDS
GCGAGTTAGGCAACTGATGC+AGG 0.458157 6.3:-6111731 None:intergenic
TTCCGACCTTTACCTCCAGC+TGG 0.468020 6.3:+6111903 MS.gene20469:CDS
GCATGGCCAGCTGGAGGTAA+AGG 0.470453 6.3:-6111909 None:intergenic
GCATTTGCTGCAATAGTAGC+GGG 0.472527 6.3:-6111447 None:intergenic
AAAACTGATGAAGAATTTGT+TGG 0.474608 6.3:-6111237 None:intergenic
CCCTGCGGAGGTAACTAAAT+CGG 0.476425 6.3:+6112058 MS.gene20469:CDS
CTTTGGTCTGGCATAGGAAG+TGG 0.476736 6.3:-6111864 None:intergenic
AAAGGAAGAAGATGCTTGCC+AGG 0.480340 6.3:+6111205 None:intergenic
GGCTTCCCAGTTGCTGGTGT+CGG 0.485684 6.3:-6111426 None:intergenic
GCCGATTTAGTTACCTCCGC+AGG 0.485908 6.3:-6112092 None:intergenic
GGCATAGACAGGTCGCAGTT+AGG 0.489584 6.3:+6112683 MS.gene20469:CDS
GACACATTAGGACCAGGATT+CGG 0.491699 6.3:-6111954 None:intergenic
GCGCCTAATGGAATAACAAC+AGG 0.497466 6.3:+6112662 MS.gene20469:CDS
ATTCTACCTCATCCGAATCC+TGG 0.497538 6.3:+6111942 MS.gene20469:CDS
CCGATTCTTTGGTCTGGCAT+AGG 0.499828 6.3:-6111870 None:intergenic
TGGGGTAGAGCATGGCCAGC+TGG 0.501385 6.3:-6111918 None:intergenic
CGAAGAGGAGGAGTTCAATT+GGG 0.502140 6.3:+6111392 MS.gene20469:CDS
TTGGGAGTCAAGTCAGCTCC+AGG 0.502567 6.3:+6112476 MS.gene20469:CDS
ACGGAGGAGTGGATGTATAG+AGG 0.502902 6.3:+6112623 MS.gene20469:CDS
GGCCTCGTTTCAAAAGTTGC+TGG 0.503897 6.3:-6111537 None:intergenic
AGCTAATGAGATAACACCTT+GGG 0.504555 6.3:-6112027 None:intergenic
TTAGCATTTATTGATTCGAA+TGG 0.504936 6.3:-6111621 None:intergenic
CCACAAGTTGTGCACTGTCT+AGG 0.505428 6.3:-6112311 None:intergenic
TCACTGTAAAACTCCTCAAA+AGG 0.506831 6.3:-6112596 None:intergenic
ATCCATCGAAGAATCCTGTT+AGG 0.507185 6.3:+6111595 MS.gene20469:CDS
GCCGATTTAGTTACCTCCGC+AGG 0.507279 6.3:-6112059 None:intergenic
ATAGCTCTAATTGATGCCTA+TGG 0.514260 6.3:+6111333 MS.gene20469:CDS
AGCACAGCAGTCACATGGAT+TGG 0.529243 6.3:+6112357 MS.gene20469:CDS
CAAAATACATGTGCATTATG+TGG 0.529245 6.3:+6112569 MS.gene20469:CDS
ATTGATTTAGTTACCTCCGC+AGG 0.532041 6.3:-6112125 None:intergenic
TGCAACTTCTTCAGCGTCAA+GGG 0.532463 6.3:+6111467 MS.gene20469:CDS
ACAGGTCGTGCATTTGGCAG+GGG 0.537469 6.3:-6111837 None:intergenic
CCTAGACAGTGCACAACTTG+TGG 0.539415 6.3:+6112311 MS.gene20469:CDS
GGCGGTGTTTGTCCACTGGT+TGG 0.543540 6.3:-6111753 None:intergenic
AACAGGTCGTGCATTTGGCA+GGG 0.545446 6.3:-6111838 None:intergenic
GAAAGGCGGTGTTTGTCCAC+TGG 0.548147 6.3:-6111757 None:intergenic
GAATAACAACAGGCATAGAC+AGG 0.548628 6.3:+6112672 MS.gene20469:CDS
AAATTAGAATATCCGACAGT+TGG 0.551878 6.3:-6111987 None:intergenic
AGCAGAAACCCTCTCGCAAG+TGG 0.553269 6.3:+6112414 MS.gene20469:CDS
AATATCCGACAGTTGGCTGT+TGG 0.554695 6.3:-6111980 None:intergenic
TCTGCATTTAGCATGAATAA+CGG 0.555648 6.3:-6112708 None:intergenic
CCTATGCCAGACCAAAGAAT+CGG 0.556854 6.3:+6111870 MS.gene20469:CDS
ATTAGTTCACTCGTGGCCCA+AGG 0.558294 6.3:+6112011 MS.gene20469:CDS
CAGGTCGTGCATTTGGCAGG+GGG 0.562977 6.3:-6111836 None:intergenic
TTCCTAACAGGATTCTTCGA+TGG 0.563320 6.3:-6111597 None:intergenic
ATGCAACTTCTTCAGCGTCA+AGG 0.564208 6.3:+6111466 MS.gene20469:CDS
CCGATTTAGTTACCTCCGCA+GGG 0.567759 6.3:-6112091 None:intergenic
AAAGTGAATTGCGACGCTGC+TGG 0.570896 6.3:-6111801 None:intergenic
TGCATTTGCTGCAATAGTAG+CGG 0.574043 6.3:-6111448 None:intergenic
TGGCTTGACACATTAGGACC+AGG 0.576366 6.3:-6111960 None:intergenic
ATTCGGATGAGGTAGAATCT+GGG 0.576849 6.3:-6111937 None:intergenic
CTCACAAACCACTTGCGAGA+GGG 0.577171 6.3:-6112422 None:intergenic
TAATTTGATTAGTTCACTCG+TGG 0.577584 6.3:+6112004 MS.gene20469:CDS
TTAGGACCAGGATTCGGATG+AGG 0.577777 6.3:-6111948 None:intergenic
GAATATTTGGATCAAACTCA+AGG 0.580223 6.3:-6112241 None:intergenic
AGATGAGCACAGCAGTCACA+TGG 0.587427 6.3:+6112352 MS.gene20469:CDS
GTTTGATCCAAATATTCTCA+AGG 0.588305 6.3:+6112247 MS.gene20469:CDS
TCGACACCATGTTCATGCAC+AGG 0.588454 6.3:-6111684 None:intergenic
CCGATTTAGTTACCTCCGCA+GGG 0.591874 6.3:-6112058 None:intergenic
CCTATTCCTGTGCATGAACA+TGG 0.596190 6.3:+6111678 MS.gene20469:CDS
GAGTTCAGCTATCGCAGCAA+CGG 0.596554 6.3:+6111291 MS.gene20469:CDS
GTAGAATCTGGGGTAGAGCA+TGG 0.600654 6.3:-6111926 None:intergenic
CATCTTTGGATTTGAATCGG+AGG 0.601375 6.3:-6112334 None:intergenic
TTGCCTAACTCGCCAACCAG+TGG 0.608662 6.3:+6111741 MS.gene20469:CDS
AATACTACCACTGTGAGTCA+TGG 0.609522 6.3:+6111642 MS.gene20469:CDS
GACGCTGCTGGATTCTGAGA+TGG 0.609727 6.3:-6111789 None:intergenic
TTCTTCGATGGATATATAGG+AGG 0.611513 6.3:-6111585 None:intergenic
TTGATTTAGTTACCTCCGCA+GGG 0.612955 6.3:-6112124 None:intergenic
AAAGTTGCTGGCGAAACTGG+TGG 0.614136 6.3:-6111525 None:intergenic
GGCCAGCTGGAGGTAAAGGT+CGG 0.614768 6.3:-6111905 None:intergenic
GGCTAATCAAACCCCTGCGG+AGG 0.615539 6.3:+6112079 MS.gene20469:CDS
GAAAATCACCATATAAGCCA+CGG 0.623056 6.3:-6112289 None:intergenic
TGATTCCGACACCAGCAACT+GGG 0.627867 6.3:+6111421 MS.gene20469:CDS
AGGTCGTGCATTTGGCAGGG+GGG 0.628298 6.3:-6111835 None:intergenic
ATGGAAGAATACCGAAGAGG+AGG 0.631037 6.3:+6111380 MS.gene20469:CDS
GATGGATATATAGGAGGACG+TGG 0.633038 6.3:-6111579 None:intergenic
ACTCACAAACCACTTGCGAG+AGG 0.633226 6.3:-6112423 None:intergenic
ACAGTGATGAAACGGAGGAG+TGG 0.635697 6.3:+6112612 MS.gene20469:CDS
CGTCATAAATCTGTAATCCG+TGG 0.642310 6.3:+6112272 MS.gene20469:CDS
TGACTTGACTCCCAATGCCT+CGG 0.643816 6.3:-6112468 None:intergenic
TCAAGCCAACAGCCAACTGT+CGG 0.643822 6.3:+6111975 MS.gene20469:CDS
GTTGGCTGTTCCGGCCGACG+AGG 0.646045 6.3:+6112544 MS.gene20469:CDS
TAGCTAATGAGATAACACCT+TGG 0.648926 6.3:-6112028 None:intergenic
AGCTAATCAAGCCCCTGCGG+AGG 0.650230 6.3:+6112046 MS.gene20469:CDS
TTCGGATGAGGTAGAATCTG+GGG 0.651800 6.3:-6111936 None:intergenic
GGCTAATCAAGCCCCTGCGG+AGG 0.653058 6.3:+6112112 MS.gene20469:CDS
ATCGGCTAATCAAGCCCCTG+CGG 0.657358 6.3:+6112109 MS.gene20469:CDS
TCAGTATTAGAATTGAGACC+AGG 0.658819 6.3:-6111495 None:intergenic
CGATTTAGTTACCTCCGCAG+GGG 0.662302 6.3:-6112090 None:intergenic
TGCATTTAGCATGAATAACG+GGG 0.669690 6.3:-6112706 None:intergenic
GGAGCATGCACATTTACGGA+GGG 0.674855 6.3:-6111558 None:intergenic
TGATTTAGTTACCTCCGCAG+GGG 0.679811 6.3:-6112123 None:intergenic
TGGAGCATGCACATTTACGG+AGG 0.681918 6.3:-6111559 None:intergenic
GGTAGAGCATGGCCAGCTGG+AGG 0.682509 6.3:-6111915 None:intergenic
CGATTTAGTTACCTCCGCAG+GGG 0.683690 6.3:-6112057 None:intergenic
TTCATGGAAGAATACCGAAG+AGG 0.683939 6.3:+6111377 MS.gene20469:CDS
GAACATGGTGTCGAGAACAA+TGG 0.686510 6.3:+6111693 MS.gene20469:CDS
ATTAGCTAATCAAGCCCCTG+CGG 0.693399 6.3:+6112043 MS.gene20469:CDS
GATGTGGCTTAAAGGTGCCG+AGG 0.696373 6.3:+6112451 MS.gene20469:CDS
TTGCTGGCGAAACTGGTGGA+TGG 0.703445 6.3:-6111521 None:intergenic
GGTTTGTGAGTGAGACGATG+TGG 0.711325 6.3:+6112435 MS.gene20469:CDS
ATCGGCTAATCAAACCCCTG+CGG 0.717147 6.3:+6112076 MS.gene20469:CDS
AATTAGACCATGACTCACAG+TGG 0.751691 6.3:-6111649 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACATAATATAATAACAAAAT+TGG - chr6.3:6112204-6112223 None:intergenic 10.0%
!! TGTTATTATATTATGTTTGT+AGG + chr6.3:6112208-6112227 MS.gene20469:intron 15.0%
!! GTGATATAAAAATTGAACTA+AGG - chr6.3:6112151-6112170 None:intergenic 20.0%
!!! TTATGTTTGTAGGATTTTAT+TGG + chr6.3:6112218-6112237 MS.gene20469:intron 20.0%
! AAAACTGATGAAGAATTTGT+TGG - chr6.3:6111240-6111259 None:intergenic 25.0%
!!! TTAGCATTTATTGATTCGAA+TGG - chr6.3:6111624-6111643 None:intergenic 25.0%
AAAGAAAGAAGATGAAGAGT+TGG + chr6.3:6112526-6112545 MS.gene20469:CDS 30.0%
AAATTAGAATATCCGACAGT+TGG - chr6.3:6111990-6112009 None:intergenic 30.0%
CAAAATACATGTGCATTATG+TGG + chr6.3:6112569-6112588 MS.gene20469:CDS 30.0%
GAATATTTGGATCAAACTCA+AGG - chr6.3:6112244-6112263 None:intergenic 30.0%
GGTACGCAATTAAACAATAA+TGG - chr6.3:6112183-6112202 None:intergenic 30.0%
GTTTGATCCAAATATTCTCA+AGG + chr6.3:6112247-6112266 MS.gene20469:CDS 30.0%
TCTGCATTTAGCATGAATAA+CGG - chr6.3:6112711-6112730 None:intergenic 30.0%
! ATCTTCTTTCTTTTCTTCAG+TGG - chr6.3:6112519-6112538 None:intergenic 30.0%
! CACATAATGCACATGTATTT+TGG - chr6.3:6112571-6112590 None:intergenic 30.0%
! CGCAATTAAACAATAATGGA+AGG - chr6.3:6112179-6112198 None:intergenic 30.0%
! TAATTTGATTAGTTCACTCG+TGG + chr6.3:6112004-6112023 MS.gene20469:CDS 30.0%
!! TCATCAAAACATCTTTTTCG+CGG - chr6.3:6111273-6111292 None:intergenic 30.0%
AGCTAATGAGATAACACCTT+GGG - chr6.3:6112030-6112049 None:intergenic 35.0%
CGAAAAGACAAGAAACTTCA+TGG + chr6.3:6111361-6111380 MS.gene20469:CDS 35.0%
CTGCATTTAGCATGAATAAC+GGG - chr6.3:6112710-6112729 None:intergenic 35.0%
GAAAATCACCATATAAGCCA+CGG - chr6.3:6112292-6112311 None:intergenic 35.0%
GCTCATCTTTGGATTTGAAT+CGG - chr6.3:6112340-6112359 None:intergenic 35.0%
GGATTCTTCGATGGATATAT+AGG - chr6.3:6111591-6111610 None:intergenic 35.0%
TAGCTAATGAGATAACACCT+TGG - chr6.3:6112031-6112050 None:intergenic 35.0%
TAGGCATCAATTAGAGCTAT+TGG - chr6.3:6111333-6111352 None:intergenic 35.0%
TCACTGTAAAACTCCTCAAA+AGG - chr6.3:6112599-6112618 None:intergenic 35.0%
TCAGTATTAGAATTGAGACC+AGG - chr6.3:6111498-6111517 None:intergenic 35.0%
TGACGAACCTTGAGAATATT+TGG - chr6.3:6112257-6112276 None:intergenic 35.0%
TGAGATGGAAAAAACTGGAA+AGG - chr6.3:6111777-6111796 None:intergenic 35.0%
TGCATTTAGCATGAATAACG+GGG - chr6.3:6112709-6112728 None:intergenic 35.0%
TTATTCCATTAGGCGCATTA+AGG - chr6.3:6112658-6112677 None:intergenic 35.0%
TTCTTCGATGGATATATAGG+AGG - chr6.3:6111588-6111607 None:intergenic 35.0%
! AAGAGTTGTTTCCGATTCTT+TGG - chr6.3:6111884-6111903 None:intergenic 35.0%
! ATGCCTGTTGTTATTCCATT+AGG - chr6.3:6112668-6112687 None:intergenic 35.0%
! ATTATGTGGAGAACCTTTTG+AGG + chr6.3:6112583-6112602 MS.gene20469:CDS 35.0%
! GGAGTTTTACAGTGATGAAA+CGG + chr6.3:6112604-6112623 MS.gene20469:CDS 35.0%
! TTTCTTTTCTTCAGTGGTCT+CGG - chr6.3:6112513-6112532 None:intergenic 35.0%
!! ATAGCTCTAATTGATGCCTA+TGG + chr6.3:6111333-6111352 MS.gene20469:CDS 35.0%
!! GATTCTGAGATGGAAAAAAC+TGG - chr6.3:6111782-6111801 None:intergenic 35.0%
AATACTACCACTGTGAGTCA+TGG + chr6.3:6111642-6111661 MS.gene20469:CDS 40.0%
AATTAGACCATGACTCACAG+TGG - chr6.3:6111652-6111671 None:intergenic 40.0%
ATCCATCGAAGAATCCTGTT+AGG + chr6.3:6111595-6111614 MS.gene20469:CDS 40.0%
ATTCGGATGAGGTAGAATCT+GGG - chr6.3:6111940-6111959 None:intergenic 40.0%
CATCTTTGGATTTGAATCGG+AGG - chr6.3:6112337-6112356 None:intergenic 40.0%
CGTCATAAATCTGTAATCCG+TGG + chr6.3:6112272-6112291 MS.gene20469:CDS 40.0%
GAATAACAACAGGCATAGAC+AGG + chr6.3:6112672-6112691 MS.gene20469:CDS 40.0%
GATGGAAAAAACTGGAAAGG+CGG - chr6.3:6111774-6111793 None:intergenic 40.0%
GTATACCTTAATGCGCCTAA+TGG + chr6.3:6112650-6112669 MS.gene20469:CDS 40.0%
TCTGTAATCCGTGGCTTATA+TGG + chr6.3:6112281-6112300 MS.gene20469:CDS 40.0%
TTCATGGAAGAATACCGAAG+AGG + chr6.3:6111377-6111396 MS.gene20469:CDS 40.0%
TTCCTAACAGGATTCTTCGA+TGG - chr6.3:6111600-6111619 None:intergenic 40.0%
TTGATTTAGTTACCTCCGCA+GGG - chr6.3:6112127-6112146 None:intergenic 40.0%
TTGTTTCCGATTCTTTGGTC+TGG - chr6.3:6111879-6111898 None:intergenic 40.0%
! ATTGATTTAGTTACCTCCGC+AGG - chr6.3:6112128-6112147 None:intergenic 40.0%
! ATTTGTTGGTGTTTCTCTCC+TGG - chr6.3:6111226-6111245 None:intergenic 40.0%
! GATTCGAATGGATTCCTAAC+AGG - chr6.3:6111612-6111631 None:intergenic 40.0%
! GTTTTACAGTGATGAAACGG+AGG + chr6.3:6112607-6112626 MS.gene20469:CDS 40.0%
! TGCATTTGCTGCAATAGTAG+CGG - chr6.3:6111451-6111470 None:intergenic 40.0%
! TTGTCTTTTCGCACTACCAT+AGG - chr6.3:6111352-6111371 None:intergenic 40.0%
!! AGAGCTATTGGATTTTGACG+AGG - chr6.3:6111321-6111340 None:intergenic 40.0%
!!! ACATGTATTTTGGTCCTCGT+CGG - chr6.3:6112561-6112580 None:intergenic 40.0%
AATATCCGACAGTTGGCTGT+TGG - chr6.3:6111983-6112002 None:intergenic 45.0%
ACGTGGAGCATGCACATTTA+CGG - chr6.3:6111565-6111584 None:intergenic 45.0%
AGATGAAGAGTTGGCTGTTC+CGG + chr6.3:6112535-6112554 MS.gene20469:CDS 45.0%
AGTGAGACGATGTGGCTTAA+AGG + chr6.3:6112443-6112462 MS.gene20469:CDS 45.0%
ATGCAACTTCTTCAGCGTCA+AGG + chr6.3:6111466-6111485 MS.gene20469:CDS 45.0%
ATGGAAGAATACCGAAGAGG+AGG + chr6.3:6111380-6111399 MS.gene20469:CDS 45.0%
ATTAGCTAATCAAGCCCCTG+CGG + chr6.3:6112043-6112062 MS.gene20469:CDS 45.0%
ATTCTACCTCATCCGAATCC+TGG + chr6.3:6111942-6111961 MS.gene20469:CDS 45.0%
CCAATTGAACTCCTCCTCTT+CGG - chr6.3:6111394-6111413 None:intergenic 45.0%
CCATGTTCATGCACAGGAAT+AGG - chr6.3:6111681-6111700 None:intergenic 45.0%
CCTATGCCAGACCAAAGAAT+CGG + chr6.3:6111870-6111889 MS.gene20469:CDS 45.0%
CCTATTCCTGTGCATGAACA+TGG + chr6.3:6111678-6111697 MS.gene20469:CDS 45.0%
CGAAGAGGAGGAGTTCAATT+GGG + chr6.3:6111392-6111411 MS.gene20469:CDS 45.0%
GACACATTAGGACCAGGATT+CGG - chr6.3:6111957-6111976 None:intergenic 45.0%
GATGGATATATAGGAGGACG+TGG - chr6.3:6111582-6111601 None:intergenic 45.0%
GATTCGGATGAGGTAGAATC+TGG - chr6.3:6111941-6111960 None:intergenic 45.0%
GCGCCTAATGGAATAACAAC+AGG + chr6.3:6112662-6112681 MS.gene20469:CDS 45.0%
TCAAAAGTTGCTGGCGAAAC+TGG - chr6.3:6111531-6111550 None:intergenic 45.0%
TGATTTAGTTACCTCCGCAG+GGG - chr6.3:6112126-6112145 None:intergenic 45.0%
TGCAACTTCTTCAGCGTCAA+GGG + chr6.3:6111467-6111486 MS.gene20469:CDS 45.0%
TTCGGATGAGGTAGAATCTG+GGG - chr6.3:6111939-6111958 None:intergenic 45.0%
! GAACATGGTGTCGAGAACAA+TGG + chr6.3:6111693-6111712 MS.gene20469:CDS 45.0%
! GCATTTGCTGCAATAGTAGC+GGG - chr6.3:6111450-6111469 None:intergenic 45.0%
! TTTTCTTCAGTGGTCTCGGT+TGG - chr6.3:6112509-6112528 None:intergenic 45.0%
AAAGTGAATTGCGACGCTGC+TGG - chr6.3:6111804-6111823 None:intergenic 50.0%
AAAGTTGCTGGCGAAACTGG+TGG - chr6.3:6111528-6111547 None:intergenic 50.0%
AACAGGTCGTGCATTTGGCA+GGG - chr6.3:6111841-6111860 None:intergenic 50.0%
ACAGTGATGAAACGGAGGAG+TGG + chr6.3:6112612-6112631 MS.gene20469:CDS 50.0%
ACGGAGGAGTGGATGTATAG+AGG + chr6.3:6112623-6112642 MS.gene20469:CDS 50.0%
ACTCACAAACCACTTGCGAG+AGG - chr6.3:6112426-6112445 None:intergenic 50.0%
AGATGAGCACAGCAGTCACA+TGG + chr6.3:6112352-6112371 MS.gene20469:CDS 50.0%
AGCACAGCAGTCACATGGAT+TGG + chr6.3:6112357-6112376 MS.gene20469:CDS 50.0%
ATCGGCTAATCAAACCCCTG+CGG + chr6.3:6112076-6112095 MS.gene20469:CDS 50.0%
ATGATTCCGACACCAGCAAC+TGG + chr6.3:6111420-6111439 MS.gene20469:CDS 50.0%
ATTAGTTCACTCGTGGCCCA+AGG + chr6.3:6112011-6112030 MS.gene20469:CDS 50.0%
CCACAAGTTGTGCACTGTCT+AGG - chr6.3:6112314-6112333 None:intergenic 50.0%
CCCTGCGGAGGTAACTAAAT+CGG + chr6.3:6112058-6112077 MS.gene20469:CDS 50.0%
CCGAAGAGGAGGAGTTCAAT+TGG + chr6.3:6111391-6111410 MS.gene20469:CDS 50.0%
CCGATTCTTTGGTCTGGCAT+AGG - chr6.3:6111873-6111892 None:intergenic 50.0%
CCGATTTAGTTACCTCCGCA+GGG - chr6.3:6112061-6112080 None:intergenic 50.0%
CCTAGACAGTGCACAACTTG+TGG + chr6.3:6112311-6112330 MS.gene20469:CDS 50.0%
CGATTTAGTTACCTCCGCAG+GGG - chr6.3:6112060-6112079 None:intergenic 50.0%
CTCACAAACCACTTGCGAGA+GGG - chr6.3:6112425-6112444 None:intergenic 50.0%
CTTTGGTCTGGCATAGGAAG+TGG - chr6.3:6111867-6111886 None:intergenic 50.0%
GAGTTCAGCTATCGCAGCAA+CGG + chr6.3:6111291-6111310 MS.gene20469:CDS 50.0%
GGAGCATGCACATTTACGGA+GGG - chr6.3:6111561-6111580 None:intergenic 50.0%
GGCATAACAGGTCGTGCATT+TGG - chr6.3:6111846-6111865 None:intergenic 50.0%
GGCATAGGAAGTGGCATAAC+AGG - chr6.3:6111858-6111877 None:intergenic 50.0%
GGCCTCGTTTCAAAAGTTGC+TGG - chr6.3:6111540-6111559 None:intergenic 50.0%
GGTTTGTGAGTGAGACGATG+TGG + chr6.3:6112435-6112454 MS.gene20469:CDS 50.0%
GTCTCGGTTGGCAAAAAACC+TGG - chr6.3:6112497-6112516 None:intergenic 50.0%
GTGACTGCTGTGCTCATCTT+TGG - chr6.3:6112351-6112370 None:intergenic 50.0%
TAACAGGTCGTGCATTTGGC+AGG - chr6.3:6111842-6111861 None:intergenic 50.0%
TCAAGCCAACAGCCAACTGT+CGG + chr6.3:6111975-6111994 MS.gene20469:CDS 50.0%
TCGACACCATGTTCATGCAC+AGG - chr6.3:6111687-6111706 None:intergenic 50.0%
TGACTTGACTCCCAATGCCT+CGG - chr6.3:6112471-6112490 None:intergenic 50.0%
TGATTCCGACACCAGCAACT+GGG + chr6.3:6111421-6111440 MS.gene20469:CDS 50.0%
TGGAGCATGCACATTTACGG+AGG - chr6.3:6111562-6111581 None:intergenic 50.0%
TGGCTTGACACATTAGGACC+AGG - chr6.3:6111963-6111982 None:intergenic 50.0%
TTAGGACCAGGATTCGGATG+AGG - chr6.3:6111951-6111970 None:intergenic 50.0%
! GTAGAATCTGGGGTAGAGCA+TGG - chr6.3:6111929-6111948 None:intergenic 50.0%
!! CTTAAAGGTGCCGAGGCATT+GGG + chr6.3:6112458-6112477 MS.gene20469:CDS 50.0%
!! GGCTGTTGGCTTGACACATT+AGG - chr6.3:6111969-6111988 None:intergenic 50.0%
!!! CGCCAGCAACTTTTGAAACG+AGG + chr6.3:6111535-6111554 MS.gene20469:CDS 50.0%
ACAGGTCGTGCATTTGGCAG+GGG - chr6.3:6111840-6111859 None:intergenic 55.0%
AGCAGAAACCCTCTCGCAAG+TGG + chr6.3:6112414-6112433 MS.gene20469:CDS 55.0%
ATCGGCTAATCAAGCCCCTG+CGG + chr6.3:6112109-6112128 MS.gene20469:CDS 55.0%
GAAAGGCGGTGTTTGTCCAC+TGG - chr6.3:6111760-6111779 None:intergenic 55.0%
GCGAGTTAGGCAACTGATGC+AGG - chr6.3:6111734-6111753 None:intergenic 55.0%
GGCATAGACAGGTCGCAGTT+AGG + chr6.3:6112683-6112702 MS.gene20469:CDS 55.0%
TTCCGACCTTTACCTCCAGC+TGG + chr6.3:6111903-6111922 MS.gene20469:CDS 55.0%
TTGCCTAACTCGCCAACCAG+TGG + chr6.3:6111741-6111760 MS.gene20469:CDS 55.0%
TTGCTGGCGAAACTGGTGGA+TGG - chr6.3:6111524-6111543 None:intergenic 55.0%
TTGGGAGTCAAGTCAGCTCC+AGG + chr6.3:6112476-6112495 MS.gene20469:CDS 55.0%
! GACGCTGCTGGATTCTGAGA+TGG - chr6.3:6111792-6111811 None:intergenic 55.0%
! GCCGATTTAGTTACCTCCGC+AGG - chr6.3:6112062-6112081 None:intergenic 55.0%
! TGTCCACTGGTTGGCGAGTT+AGG - chr6.3:6111747-6111766 None:intergenic 55.0%
!! GATGTGGCTTAAAGGTGCCG+AGG + chr6.3:6112451-6112470 MS.gene20469:CDS 55.0%
!! GCTTAAAGGTGCCGAGGCAT+TGG + chr6.3:6112457-6112476 MS.gene20469:CDS 55.0%
!! TCAGCGTCAAGGGTGTTTCC+TGG + chr6.3:6111477-6111496 MS.gene20469:CDS 55.0%
!!! GTATTTTGGTCCTCGTCGGC+CGG - chr6.3:6112557-6112576 None:intergenic 55.0%
AGCTAATCAAGCCCCTGCGG+AGG + chr6.3:6112046-6112065 MS.gene20469:CDS 60.0%
AGGTCGTGCATTTGGCAGGG+GGG - chr6.3:6111838-6111857 None:intergenic 60.0%
CAGGTCGTGCATTTGGCAGG+GGG - chr6.3:6111839-6111858 None:intergenic 60.0%
GCATGGCCAGCTGGAGGTAA+AGG - chr6.3:6111912-6111931 None:intergenic 60.0%
GGCCAGCTGGAGGTAAAGGT+CGG - chr6.3:6111908-6111927 None:intergenic 60.0%
GGCGGTGTTTGTCCACTGGT+TGG - chr6.3:6111756-6111775 None:intergenic 60.0%
GGCTAATCAAACCCCTGCGG+AGG + chr6.3:6112079-6112098 MS.gene20469:CDS 60.0%
GGCTTCCCAGTTGCTGGTGT+CGG - chr6.3:6111429-6111448 None:intergenic 60.0%
! TTTGGCAGGGGGGCATGTCT+TGG - chr6.3:6111828-6111847 None:intergenic 60.0%
GGCTAATCAAGCCCCTGCGG+AGG + chr6.3:6112112-6112131 MS.gene20469:CDS 65.0%
GGTAGAGCATGGCCAGCTGG+AGG - chr6.3:6111918-6111937 None:intergenic 65.0%
TGGGGTAGAGCATGGCCAGC+TGG - chr6.3:6111921-6111940 None:intergenic 65.0%
!! GTAGCGGGCTTCCCAGTTGC+TGG - chr6.3:6111435-6111454 None:intergenic 65.0%
GTTGGCTGTTCCGGCCGACG+AGG + chr6.3:6112544-6112563 MS.gene20469:CDS 70.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 6111216 6112736 6111216 ID=MS.gene20469
chr6.3 mRNA 6111216 6112736 6111216 ID=MS.gene20469.t1;Parent=MS.gene20469
chr6.3 exon 6111216 6112133 6111216 ID=MS.gene20469.t1.exon1;Parent=MS.gene20469.t1
chr6.3 CDS 6111216 6112133 6111216 ID=cds.MS.gene20469.t1;Parent=MS.gene20469.t1
chr6.3 exon 6112230 6112736 6112230 ID=MS.gene20469.t1.exon2;Parent=MS.gene20469.t1
chr6.3 CDS 6112230 6112736 6112230 ID=cds.MS.gene20469.t1;Parent=MS.gene20469.t1
Gene Sequence

>MS.gene20469

ATGCTTGCCAGGAGAGAAACACCAACAAATTCTTCATCAGTTTTCTCACACTTTCCGCGAAAAAGATGTTTTGATGAGTTCAGCTATCGCAGCAACGGACAACCTCGTCAAAATCCAATAGCTCTAATTGATGCCTATGGTAGTGCGAAAAGACAAGAAACTTCATGGAAGAATACCGAAGAGGAGGAGTTCAATTGGGAAGATATGATTCCGACACCAGCAACTGGGAAGCCCGCTACTATTGCAGCAAATGCAACTTCTTCAGCGTCAAGGGTGTTTCCTGGTCTCAATTCTAATACTGAGTACCATCCACCAGTTTCGCCAGCAACTTTTGAAACGAGGCCCTCCGTAAATGTGCATGCTCCACGTCCTCCTATATATCCATCGAAGAATCCTGTTAGGAATCCATTCGAATCAATAAATGCTAATACTACCACTGTGAGTCATGGTCTAATTAATAGACCTATTCCTGTGCATGAACATGGTGTCGAGAACAATGGTATCAGTAAAAGAAACCTGCATCAGTTGCCTAACTCGCCAACCAGTGGACAAACACCGCCTTTCCAGTTTTTTCCATCTCAGAATCCAGCAGCGTCGCAATTCACTTTGCCAAGACATGCCCCCCTGCCAAATGCACGACCTGTTATGCCACTTCCTATGCCAGACCAAAGAATCGGAAACAACTCTTTCCGACCTTTACCTCCAGCTGGCCATGCTCTACCCCAGATTCTACCTCATCCGAATCCTGGTCCTAATGTGTCAAGCCAACAGCCAACTGTCGGATATTCTAATTTGATTAGTTCACTCGTGGCCCAAGGTGTTATCTCATTAGCTAATCAAGCCCCTGCGGAGGTAACTAAATCGGCTAATCAAACCCCTGCGGAGGTAACTAAATCGGCTAATCAAGCCCCTGCGGAGGTAACTAAATCAATCCTTAGTTCAATTTTTATATCACTTTTTCCTTCCATTATTGTTTAATTGCGTACCAATTTTGTTATTATATTATGTTTGTAGGATTTTATTGGCCTTGAGTTTGATCCAAATATTCTCAAGGTTCGTCATAAATCTGTAATCCGTGGCTTATATGGTGATTTTCCTAGACAGTGCACAACTTGTGGCCTCCGATTCAAATCCAAAGATGAGCACAGCAGTCACATGGATTGGCATGTAACTAAGAATCGTATGTCTAAAAACAATAAGCAGAAACCCTCTCGCAAGTGGTTTGTGAGTGAGACGATGTGGCTTAAAGGTGCCGAGGCATTGGGAGTCAAGTCAGCTCCAGGTTTTTTGCCAACCGAGACCACTGAAGAAAAGAAAGAAGATGAAGAGTTGGCTGTTCCGGCCGACGAGGACCAAAATACATGTGCATTATGTGGAGAACCTTTTGAGGAGTTTTACAGTGATGAAACGGAGGAGTGGATGTATAGAGGAGCTGTATACCTTAATGCGCCTAATGGAATAACAACAGGCATAGACAGGTCGCAGTTAGGCCCCGTTATTCATGCTAAATGCAGATCTTAA
Protein sequence

>MS.gene20469.t1

MLARRETPTNSSSVFSHFPRKRCFDEFSYRSNGQPRQNPIALIDAYGSAKRQETSWKNTEEEEFNWEDMIPTPATGKPATIAANATSSASRVFPGLNSNTEYHPPVSPATFETRPSVNVHAPRPPIYPSKNPVRNPFESINANTTTVSHGLINRPIPVHEHGVENNGISKRNLHQLPNSPTSGQTPPFQFFPSQNPAASQFTLPRHAPLPNARPVMPLPMPDQRIGNNSFRPLPPAGHALPQILPHPNPGPNVSSQQPTVGYSNLISSLVAQGVISLANQAPAEVTKSANQTPAEVTKSANQAPAEDFIGLEFDPNILKVRHKSVIRGLYGDFPRQCTTCGLRFKSKDEHSSHMDWHVTKNRMSKNNKQKPSRKWFVSETMWLKGAEALGVKSAPGFLPTETTEEKKEDEELAVPADEDQNTCALCGEPFEEFYSDETEEWMYRGAVYLNAPNGITTGIDRSQLGPVIHAKCRS