Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20568.t1 | KEH40197.1 | 94.1 | 68 | 4 | 0 | 1 | 68 | 1 | 68 | 1.10E-30 | 142.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20568.t1 | Q9LQZ7 | 68.8 | 64 | 18 | 1 | 1 | 62 | 1 | 64 | 1.1e-18 | 93.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20568.t1 | A0A072VEF4 | 94.1 | 68 | 4 | 0 | 1 | 68 | 1 | 68 | 7.8e-31 | 142.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene20568.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20568.t1 | MTR_1g023260 | 94.118 | 68 | 4 | 0 | 1 | 68 | 1 | 68 | 3.77e-42 | 135 |
| MS.gene20568.t1 | MTR_1g023260 | 94.118 | 68 | 4 | 0 | 1 | 68 | 1 | 68 | 4.21e-42 | 138 |
| MS.gene20568.t1 | MTR_3g113070 | 72.464 | 69 | 18 | 1 | 1 | 68 | 1 | 69 | 1.08e-27 | 99.8 |
| MS.gene20568.t1 | MTR_4g071200 | 60.294 | 68 | 27 | 0 | 1 | 68 | 1 | 68 | 3.51e-24 | 90.9 |
| MS.gene20568.t1 | MTR_3g117320 | 64.516 | 62 | 22 | 0 | 1 | 62 | 1 | 62 | 1.66e-22 | 86.7 |
| MS.gene20568.t1 | MTR_2g073370 | 59.677 | 62 | 24 | 1 | 1 | 62 | 1 | 61 | 3.48e-19 | 79.0 |
| MS.gene20568.t1 | MTR_2g099010 | 58.824 | 68 | 27 | 1 | 1 | 68 | 1 | 67 | 2.97e-18 | 75.9 |
| MS.gene20568.t1 | MTR_2g099010 | 58.824 | 68 | 27 | 1 | 1 | 68 | 1 | 67 | 3.25e-18 | 75.5 |
| MS.gene20568.t1 | MTR_4g067320 | 59.155 | 71 | 22 | 2 | 1 | 68 | 1 | 67 | 7.81e-18 | 74.7 |
| MS.gene20568.t1 | MTR_4g067320 | 59.155 | 71 | 22 | 2 | 1 | 68 | 1 | 67 | 8.35e-18 | 74.3 |
| MS.gene20568.t1 | MTR_5g021580 | 49.254 | 67 | 32 | 1 | 1 | 67 | 1 | 65 | 1.41e-14 | 65.1 |
| MS.gene20568.t1 | MTR_5g021580 | 49.254 | 67 | 32 | 1 | 1 | 67 | 1 | 65 | 1.72e-14 | 65.1 |
| MS.gene20568.t1 | MTR_2g011450 | 43.284 | 67 | 27 | 2 | 1 | 67 | 1 | 56 | 2.23e-11 | 55.5 |
| MS.gene20568.t1 | MTR_1g013450 | 55.814 | 43 | 19 | 0 | 5 | 47 | 6 | 48 | 6.93e-11 | 56.2 |
| MS.gene20568.t1 | MTR_4g128930 | 41.379 | 58 | 24 | 1 | 5 | 62 | 22 | 69 | 8.19e-11 | 56.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20568.t1 | AT1G75540 | 68.750 | 64 | 18 | 1 | 1 | 62 | 1 | 64 | 6.44e-25 | 94.7 |
| MS.gene20568.t1 | AT4G39070 | 67.213 | 61 | 20 | 0 | 1 | 61 | 1 | 61 | 6.47e-21 | 82.8 |
| MS.gene20568.t1 | AT1G06040 | 58.824 | 68 | 27 | 1 | 1 | 68 | 1 | 67 | 5.30e-19 | 76.3 |
| MS.gene20568.t1 | AT1G06040 | 58.824 | 68 | 27 | 1 | 1 | 68 | 1 | 67 | 2.35e-18 | 76.3 |
| MS.gene20568.t1 | AT1G78600 | 55.224 | 67 | 29 | 1 | 1 | 67 | 1 | 66 | 4.48e-18 | 76.3 |
| MS.gene20568.t1 | AT2G31380 | 60.294 | 68 | 26 | 1 | 1 | 68 | 1 | 67 | 6.89e-18 | 74.7 |
| MS.gene20568.t1 | AT1G78600 | 55.224 | 67 | 29 | 1 | 1 | 67 | 1 | 66 | 1.04e-17 | 75.1 |
| MS.gene20568.t1 | AT4G10240 | 55.224 | 67 | 25 | 1 | 1 | 62 | 1 | 67 | 7.95e-17 | 70.5 |
| MS.gene20568.t1 | AT2G21320 | 50.746 | 67 | 31 | 1 | 1 | 67 | 1 | 65 | 1.36e-15 | 67.4 |
| MS.gene20568.t1 | AT4G38960 | 50.746 | 67 | 31 | 1 | 1 | 67 | 1 | 65 | 1.57e-15 | 67.4 |
| MS.gene20568.t1 | AT4G38960 | 50.746 | 67 | 31 | 1 | 1 | 67 | 1 | 65 | 1.57e-15 | 67.4 |
| MS.gene20568.t1 | AT2G24790 | 46.552 | 58 | 21 | 1 | 5 | 62 | 8 | 55 | 9.38e-12 | 58.9 |
| MS.gene20568.t1 | AT2G24790 | 46.552 | 58 | 21 | 1 | 5 | 62 | 8 | 55 | 4.34e-11 | 56.2 |
Find 18 sgRNAs with CRISPR-Local
Find 19 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAGGATGTTGTTTAGGAGTT+GGG | 0.321006 | 6.3:+4758628 | None:intergenic |
| ACAAAATATAAGAGAAAGTT+AGG | 0.330262 | 6.3:+4758568 | None:intergenic |
| TCGCAGAGAGGATGTTGTTT+AGG | 0.354430 | 6.3:+4758621 | None:intergenic |
| AAGAGCGAGACGGTGGTGTT+TGG | 0.393267 | 6.3:+4758656 | None:intergenic |
| AGAGGATGTTGTTTAGGAGT+TGG | 0.413569 | 6.3:+4758627 | None:intergenic |
| TCCTCTCTGCGATATTTGTC+AGG | 0.454584 | 6.3:-4758610 | MS.gene20568:CDS |
| GATACATAAGGCGGCTTCAT+CGG | 0.502006 | 6.3:+4758719 | None:intergenic |
| GTGTTTGGAAGCGAGTTTGT+TGG | 0.511969 | 6.3:+4758671 | None:intergenic |
| GTGGTCACAGTCGATACATA+AGG | 0.545496 | 6.3:+4758707 | None:intergenic |
| GTTTGTTGGCGTGGTGGACA+CGG | 0.549454 | 6.3:+4758685 | None:intergenic |
| TGGAAGCGAGTTTGTTGGCG+TGG | 0.613525 | 6.3:+4758676 | None:intergenic |
| ACATAAGGCGGCTTCATCGG+CGG | 0.633059 | 6.3:+4758722 | None:intergenic |
| AAGCGAGTTTGTTGGCGTGG+TGG | 0.633769 | 6.3:+4758679 | None:intergenic |
| TTGGGTTATGAAGAGCGAGA+CGG | 0.678930 | 6.3:+4758646 | None:intergenic |
| GGTTATGAAGAGCGAGACGG+TGG | 0.682697 | 6.3:+4758649 | None:intergenic |
| GTCACAGTCGATACATAAGG+CGG | 0.712524 | 6.3:+4758710 | None:intergenic |
| TGTTGGCGTGGTGGACACGG+TGG | 0.723732 | 6.3:+4758688 | None:intergenic |
| ACCTGACAAATATCGCAGAG+AGG | 0.733622 | 6.3:+4758609 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ACAAAATATAAGAGAAAGTT+AGG | + | chr6.3:4758767-4758786 | None:intergenic | 20.0% |
| ! | TTTTGTTGCATACATCACAC+TGG | + | chr6.3:4758569-4758588 | None:intergenic | 35.0% |
| ! | AGAGGATGTTGTTTAGGAGT+TGG | + | chr6.3:4758708-4758727 | None:intergenic | 40.0% |
| ! | GAGGATGTTGTTTAGGAGTT+GGG | + | chr6.3:4758707-4758726 | None:intergenic | 40.0% |
| ACCTGACAAATATCGCAGAG+AGG | + | chr6.3:4758726-4758745 | None:intergenic | 45.0% | |
| GATACATAAGGCGGCTTCAT+CGG | + | chr6.3:4758616-4758635 | None:intergenic | 45.0% | |
| GTCACAGTCGATACATAAGG+CGG | + | chr6.3:4758625-4758644 | None:intergenic | 45.0% | |
| GTGGTCACAGTCGATACATA+AGG | + | chr6.3:4758628-4758647 | None:intergenic | 45.0% | |
| GTGTTTGGAAGCGAGTTTGT+TGG | + | chr6.3:4758664-4758683 | None:intergenic | 45.0% | |
| TCCTCTCTGCGATATTTGTC+AGG | - | chr6.3:4758722-4758741 | MS.gene20568:CDS | 45.0% | |
| TTGGGTTATGAAGAGCGAGA+CGG | + | chr6.3:4758689-4758708 | None:intergenic | 45.0% | |
| ! | TCGCAGAGAGGATGTTGTTT+AGG | + | chr6.3:4758714-4758733 | None:intergenic | 45.0% |
| AAGAGCGAGACGGTGGTGTT+TGG | + | chr6.3:4758679-4758698 | None:intergenic | 55.0% | |
| ACATAAGGCGGCTTCATCGG+CGG | + | chr6.3:4758613-4758632 | None:intergenic | 55.0% | |
| GGTTATGAAGAGCGAGACGG+TGG | + | chr6.3:4758686-4758705 | None:intergenic | 55.0% | |
| !! | AAGCGAGTTTGTTGGCGTGG+TGG | + | chr6.3:4758656-4758675 | None:intergenic | 55.0% |
| !! | GTTTGTTGGCGTGGTGGACA+CGG | + | chr6.3:4758650-4758669 | None:intergenic | 55.0% |
| !! | TGGAAGCGAGTTTGTTGGCG+TGG | + | chr6.3:4758659-4758678 | None:intergenic | 55.0% |
| !! | TGTTGGCGTGGTGGACACGG+TGG | + | chr6.3:4758647-4758666 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 4758558 | 4758796 | 4758558 | ID=MS.gene20568 |
| chr6.3 | mRNA | 4758558 | 4758796 | 4758558 | ID=MS.gene20568.t1;Parent=MS.gene20568 |
| chr6.3 | exon | 4758558 | 4758796 | 4758558 | ID=MS.gene20568.t1.exon1;Parent=MS.gene20568.t1 |
| chr6.3 | CDS | 4758558 | 4758796 | 4758558 | ID=cds.MS.gene20568.t1;Parent=MS.gene20568.t1 |
| Gene Sequence |
| Protein sequence |
