Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20578.t1 | XP_003607052.1 | 98.7 | 151 | 2 | 0 | 1 | 151 | 1 | 151 | 3.50E-78 | 300.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20578.t1 | F4JZT3 | 33.3 | 105 | 70 | 0 | 33 | 137 | 18 | 122 | 1.5e-13 | 77.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20578.t1 | G7JJU6 | 98.7 | 151 | 2 | 0 | 1 | 151 | 1 | 151 | 2.5e-78 | 300.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene20578.t1 | TR | Others |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20578.t1 | MTR_6g008860 | 98.675 | 151 | 2 | 0 | 1 | 151 | 1 | 151 | 1.99e-110 | 310 |
| MS.gene20578.t1 | MTR_4g071650 | 98.675 | 151 | 2 | 0 | 1 | 151 | 1 | 151 | 1.99e-110 | 310 |
| MS.gene20578.t1 | MTR_7g089310 | 83.721 | 43 | 7 | 0 | 102 | 144 | 1 | 43 | 7.47e-19 | 76.3 |
| MS.gene20578.t1 | MTR_7g099500 | 34.211 | 114 | 75 | 0 | 33 | 146 | 11 | 124 | 2.18e-18 | 76.3 |
| MS.gene20578.t1 | MTR_7g098910 | 34.513 | 113 | 73 | 1 | 25 | 137 | 14 | 125 | 9.46e-18 | 75.1 |
| MS.gene20578.t1 | MTR_8g063580 | 35.185 | 108 | 68 | 1 | 33 | 138 | 12 | 119 | 1.71e-15 | 69.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene20578.t1 | AT5G26594 | 32.143 | 112 | 76 | 0 | 33 | 144 | 18 | 129 | 1.01e-17 | 74.7 |
Find 28 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCTCTCGGGTCAAAGGTTT+TGG | 0.227586 | 6.3:-4649826 | None:intergenic |
| TACCTCGGGTCCATTGATTA+TGG | 0.250485 | 6.3:-4649123 | None:intergenic |
| AGCAATGCATTCATTGGATA+TGG | 0.328332 | 6.3:-4648948 | None:intergenic |
| GCTTCTTGAGCACTATTTGC+AGG | 0.339727 | 6.3:-4649041 | None:intergenic |
| CACTATTTGCAGGCTCTGTT+CGG | 0.378455 | 6.3:-4649031 | None:intergenic |
| CATCATATGCTTGAATCAAT+AGG | 0.390164 | 6.3:+4649005 | MS.gene20578:CDS |
| TTTGATTTGCCCATAATCAA+TGG | 0.409566 | 6.3:+4649113 | MS.gene20578:CDS |
| GATCAAAATGTGCAAATGTT+TGG | 0.412226 | 6.3:+4649778 | MS.gene20578:CDS |
| ACCTCGGGTCCATTGATTAT+GGG | 0.437317 | 6.3:-4649122 | None:intergenic |
| TCTACTAGCAATGCATTCAT+TGG | 0.442738 | 6.3:-4648954 | None:intergenic |
| GTGAGGCAGATGCGTGCAAT+GGG | 0.459873 | 6.3:+4649715 | MS.gene20578:CDS |
| CATATGATGCTCTAAGCGTC+TGG | 0.510351 | 6.3:-4648991 | None:intergenic |
| TGATGAACACAAAACTCTCT+CGG | 0.519844 | 6.3:-4649840 | None:intergenic |
| ACAAAACTCTCTCGGGTCAA+AGG | 0.520328 | 6.3:-4649832 | None:intergenic |
| ATGAATGCATTGCTAGTAGA+TGG | 0.520529 | 6.3:+4648957 | MS.gene20578:CDS |
| GATGAACACAAAACTCTCTC+GGG | 0.532140 | 6.3:-4649839 | None:intergenic |
| AAGTTGCAATCAACAATGGA+TGG | 0.566106 | 6.3:+4648846 | None:intergenic |
| GGGATACAGAGCAAGGTGGT+TGG | 0.588650 | 6.3:+4649736 | MS.gene20578:CDS |
| GGTGAGGCAGATGCGTGCAA+TGG | 0.598157 | 6.3:+4649714 | MS.gene20578:CDS |
| AACAATGGATGGAGGAACCA+CGG | 0.600766 | 6.3:+4648857 | None:intergenic |
| TGCAATGGGGATACAGAGCA+AGG | 0.629234 | 6.3:+4649729 | MS.gene20578:CDS |
| ACAATGGATGGAGGAACCAC+GGG | 0.635016 | 6.3:+4648858 | None:intergenic |
| GCCCATAATCAATGGACCCG+AGG | 0.655096 | 6.3:+4649121 | MS.gene20578:CDS |
| GAGGAACCACGGGATATAAG+CGG | 0.658571 | 6.3:+4648868 | MS.gene20578:CDS |
| ATATGCATTAGTTTCACATG+CGG | 0.660237 | 6.3:-4649876 | None:intergenic |
| TTGCAATCAACAATGGATGG+AGG | 0.668830 | 6.3:+4648849 | None:intergenic |
| TGAGGCAGATGCGTGCAATG+GGG | 0.781176 | 6.3:+4649716 | MS.gene20578:CDS |
| AATGGGGATACAGAGCAAGG+TGG | 0.782718 | 6.3:+4649732 | MS.gene20578:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AATATATAAGTAATTTTGTC+CGG | - | chr6.3:4649317-4649336 | None:intergenic | 15.0% |
| !!! | TAAATAAATTTTTGATACCT+CGG | - | chr6.3:4649141-4649160 | None:intergenic | 15.0% |
| !! | AAAATTGAGAACTCATTTAT+GGG | - | chr6.3:4649614-4649633 | None:intergenic | 20.0% |
| !! | ATTACTTATATATTAACCAC+TGG | + | chr6.3:4649323-4649342 | MS.gene20578:intron | 20.0% |
| !! | TAAAATTGAGAACTCATTTA+TGG | - | chr6.3:4649615-4649634 | None:intergenic | 20.0% |
| !!! | AAATAAATTTTTGATACCTC+GGG | - | chr6.3:4649140-4649159 | None:intergenic | 20.0% |
| !!! | ATATATAAGTAATTTTGTCC+GGG | - | chr6.3:4649316-4649335 | None:intergenic | 20.0% |
| ! | CAGTTAATCTCATATTTAGA+CGG | + | chr6.3:4649244-4649263 | MS.gene20578:intron | 25.0% |
| !! | CAACTAGTTAGTTTAAAATG+TGG | - | chr6.3:4649198-4649217 | None:intergenic | 25.0% |
| !!! | GGATCTATTTTGATCATATA+TGG | - | chr6.3:4649453-4649472 | None:intergenic | 25.0% |
| !!! | TTCCTAATTACTTTTGTAGT+TGG | + | chr6.3:4649693-4649712 | MS.gene20578:intron | 25.0% |
| ACTTATATATTAACCACTGG+CGG | + | chr6.3:4649326-4649345 | MS.gene20578:intron | 30.0% | |
| ATCCTTTGTCATCAACTTTA+TGG | + | chr6.3:4649469-4649488 | MS.gene20578:intron | 30.0% | |
| CACCAACTACAAAAGTAATT+AGG | - | chr6.3:4649698-4649717 | None:intergenic | 30.0% | |
| CATCATATGCTTGAATCAAT+AGG | + | chr6.3:4649005-4649024 | MS.gene20578:CDS | 30.0% | |
| GATCAAAATGTGCAAATGTT+TGG | + | chr6.3:4649778-4649797 | MS.gene20578:CDS | 30.0% | |
| TAATCTCATATTTAGACGGT+GGG | + | chr6.3:4649248-4649267 | MS.gene20578:intron | 30.0% | |
| TGAGATGAATTCTTATCAAC+TGG | + | chr6.3:4649396-4649415 | MS.gene20578:intron | 30.0% | |
| TTAATCTCATATTTAGACGG+TGG | + | chr6.3:4649247-4649266 | MS.gene20578:intron | 30.0% | |
| !! | AATTACTTTTGTAGTTGGTG+AGG | + | chr6.3:4649698-4649717 | MS.gene20578:intron | 30.0% |
| !! | TACCATAAAGTTGATGACAA+AGG | - | chr6.3:4649474-4649493 | None:intergenic | 30.0% |
| !! | TTTGATTTGCCCATAATCAA+TGG | + | chr6.3:4649113-4649132 | MS.gene20578:CDS | 30.0% |
| !!! | TCAACTGGACTATTTTTTGT+TGG | + | chr6.3:4649411-4649430 | MS.gene20578:intron | 30.0% |
| AGCAATGCATTCATTGGATA+TGG | - | chr6.3:4648951-4648970 | None:intergenic | 35.0% | |
| ATGAATGCATTGCTAGTAGA+TGG | + | chr6.3:4648957-4648976 | MS.gene20578:CDS | 35.0% | |
| ATTTATGGGCTTGGATTTGA+CGG | - | chr6.3:4649600-4649619 | None:intergenic | 35.0% | |
| TCTACTAGCAATGCATTCAT+TGG | - | chr6.3:4648957-4648976 | None:intergenic | 35.0% | |
| TCTCACGTATTTCTTTGTTC+TGG | - | chr6.3:4649286-4649305 | None:intergenic | 35.0% | |
| TGATGAACACAAAACTCTCT+CGG | - | chr6.3:4649843-4649862 | None:intergenic | 35.0% | |
| !! | AGAGTTTTGTGTTCATCACT+GGG | + | chr6.3:4649845-4649864 | MS.gene20578:CDS | 35.0% |
| !!! | ATCCACATTTTTTTCTCCTC+AGG | - | chr6.3:4648903-4648922 | None:intergenic | 35.0% |
| AATCAAACAACCCCACTTGA+TGG | + | chr6.3:4649544-4649563 | MS.gene20578:intron | 40.0% | |
| AGCCTGAGGAGAAAAAAATG+TGG | + | chr6.3:4648898-4648917 | MS.gene20578:CDS | 40.0% | |
| ATATTTAGACGGTGGGTCTT+TGG | + | chr6.3:4649255-4649274 | MS.gene20578:intron | 40.0% | |
| GATGAACACAAAACTCTCTC+GGG | - | chr6.3:4649842-4649861 | None:intergenic | 40.0% | |
| TATTTAGACGGTGGGTCTTT+GGG | + | chr6.3:4649256-4649275 | MS.gene20578:intron | 40.0% | |
| TGAAACTCATCGTCAGTATG+AGG | - | chr6.3:4649355-4649374 | None:intergenic | 40.0% | |
| TGAGAACTCATTTATGGGCT+TGG | - | chr6.3:4649609-4649628 | None:intergenic | 40.0% | |
| ! | GCGATGCAAATTTTGTTGAC+TGG | + | chr6.3:4649062-4649081 | MS.gene20578:CDS | 40.0% |
| !! | GAGAGTTTTGTGTTCATCAC+TGG | + | chr6.3:4649844-4649863 | MS.gene20578:CDS | 40.0% |
| !! | TCAAGTTGACTCCATCAAGT+GGG | - | chr6.3:4649558-4649577 | None:intergenic | 40.0% |
| AATACGTGAGACTCAGATCC+CGG | + | chr6.3:4649295-4649314 | MS.gene20578:intron | 45.0% | |
| ACAAAACTCTCTCGGGTCAA+AGG | - | chr6.3:4649835-4649854 | None:intergenic | 45.0% | |
| CACTATTTGCAGGCTCTGTT+CGG | - | chr6.3:4649034-4649053 | None:intergenic | 45.0% | |
| GCTTCTTGAGCACTATTTGC+AGG | - | chr6.3:4649044-4649063 | None:intergenic | 45.0% | |
| TAAGCGGCAAAAAAAGCCTG+AGG | + | chr6.3:4648884-4648903 | MS.gene20578:CDS | 45.0% | |
| ! | ACCTCGGGTCCATTGATTAT+GGG | - | chr6.3:4649125-4649144 | None:intergenic | 45.0% |
| ! | CAAGTTGACTCCATCAAGTG+GGG | - | chr6.3:4649557-4649576 | None:intergenic | 45.0% |
| ! | CATATGATGCTCTAAGCGTC+TGG | - | chr6.3:4648994-4649013 | None:intergenic | 45.0% |
| ! | TACCTCGGGTCCATTGATTA+TGG | - | chr6.3:4649126-4649145 | None:intergenic | 45.0% |
| !! | GTCAAGTTGACTCCATCAAG+TGG | - | chr6.3:4649559-4649578 | None:intergenic | 45.0% |
| !! | TTTTTGCCGCTTATATCCCG+TGG | - | chr6.3:4648877-4648896 | None:intergenic | 45.0% |
| AATGGGGATACAGAGCAAGG+TGG | + | chr6.3:4649732-4649751 | MS.gene20578:CDS | 50.0% | |
| GAGGAACCACGGGATATAAG+CGG | + | chr6.3:4648868-4648887 | MS.gene20578:CDS | 50.0% | |
| TGCAATGGGGATACAGAGCA+AGG | + | chr6.3:4649729-4649748 | MS.gene20578:CDS | 50.0% | |
| ! | CTCTCTCGGGTCAAAGGTTT+TGG | - | chr6.3:4649829-4649848 | None:intergenic | 50.0% |
| !!! | GGTCAAAGGTTTTGGAGTGC+TGG | - | chr6.3:4649821-4649840 | None:intergenic | 50.0% |
| CAGTATGAGGATACCGCCAG+TGG | - | chr6.3:4649342-4649361 | None:intergenic | 55.0% | |
| GCCCATAATCAATGGACCCG+AGG | + | chr6.3:4649121-4649140 | MS.gene20578:CDS | 55.0% | |
| GGGATACAGAGCAAGGTGGT+TGG | + | chr6.3:4649736-4649755 | MS.gene20578:CDS | 55.0% | |
| GTGAGGCAGATGCGTGCAAT+GGG | + | chr6.3:4649715-4649734 | MS.gene20578:CDS | 55.0% | |
| TGAGGCAGATGCGTGCAATG+GGG | + | chr6.3:4649716-4649735 | MS.gene20578:CDS | 55.0% | |
| GGTGAGGCAGATGCGTGCAA+TGG | + | chr6.3:4649714-4649733 | MS.gene20578:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.3 | gene | 4648861 | 4649885 | 4648861 | ID=MS.gene20578 |
| chr6.3 | mRNA | 4648861 | 4649885 | 4648861 | ID=MS.gene20578.t1;Parent=MS.gene20578 |
| chr6.3 | exon | 4648861 | 4649142 | 4648861 | ID=MS.gene20578.t1.exon1;Parent=MS.gene20578.t1 |
| chr6.3 | CDS | 4648861 | 4649142 | 4648861 | ID=cds.MS.gene20578.t1;Parent=MS.gene20578.t1 |
| chr6.3 | exon | 4649712 | 4649885 | 4649712 | ID=MS.gene20578.t1.exon2;Parent=MS.gene20578.t1 |
| chr6.3 | CDS | 4649712 | 4649885 | 4649712 | ID=cds.MS.gene20578.t1;Parent=MS.gene20578.t1 |
| Gene Sequence |
| Protein sequence |
