AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene20860

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20860.t1 MTR_1g086970 97.292 480 13 0 8 487 1 480 0.0 971
MS.gene20860.t1 MTR_7g109580 58.974 468 171 10 29 487 101 556 0.0 525
MS.gene20860.t1 MTR_2g034250 45.272 497 242 10 15 487 97 587 2.25e-130 390
MS.gene20860.t1 MTR_2g034260 44.534 494 250 10 6 487 105 586 1.04e-129 389
MS.gene20860.t1 MTR_2g034280 44.490 490 241 9 20 487 97 577 8.56e-127 381
MS.gene20860.t1 MTR_4g104020 30.691 391 244 13 108 485 144 520 7.30e-42 156
MS.gene20860.t1 MTR_7g027190 29.219 397 260 11 100 485 287 673 1.98e-40 154
MS.gene20860.t1 MTR_3g065980 26.161 409 256 10 99 486 158 541 2.72e-39 150
MS.gene20860.t1 MTR_8g442410 29.136 405 263 12 92 485 134 525 2.75e-38 147
MS.gene20860.t1 MTR_7g062120 28.351 388 254 11 108 485 367 740 4.80e-34 136
MS.gene20860.t1 MTR_4g102790 23.590 390 276 10 105 484 284 661 6.02e-32 130
MS.gene20860.t1 MTR_5g009080 26.078 464 253 15 91 485 36 478 1.49e-30 124
MS.gene20860.t1 MTR_4g064200 24.936 393 257 12 108 484 279 649 3.38e-29 121
MS.gene20860.t1 MTR_4g064150 25.879 398 263 11 103 484 351 732 2.51e-28 119
MS.gene20860.t1 MTR_7g074650 26.558 369 251 11 118 485 443 792 1.32e-27 117
MS.gene20860.t1 MTR_2g026250 25.714 350 237 9 146 485 260 596 1.78e-27 116
MS.gene20860.t1 MTR_4g064120 24.422 389 264 9 102 475 243 616 2.69e-27 115
MS.gene20860.t1 MTR_2g097410 25.127 394 259 12 108 484 366 740 2.45e-26 113
MS.gene20860.t1 MTR_3g056110 27.042 355 226 15 146 485 206 542 2.88e-26 112
MS.gene20860.t1 MTR_4g064160 24.936 389 264 12 108 484 311 683 4.80e-26 112
MS.gene20860.t1 MTR_2g097310 24.235 392 261 14 108 484 267 637 7.55e-26 111
MS.gene20860.t1 MTR_4g064180 24.623 398 252 12 102 475 243 616 3.30e-25 109
MS.gene20860.t1 MTR_2g097463 23.350 394 261 10 108 484 285 654 1.97e-24 107
MS.gene20860.t1 MTR_4g133660 24.069 349 242 8 146 484 207 542 5.35e-24 105
MS.gene20860.t1 MTR_2g097390 24.318 440 284 15 67 484 228 640 2.95e-23 103
MS.gene20860.t1 MTR_4g097080 24.090 357 254 7 138 485 155 503 3.53e-23 102
MS.gene20860.t1 MTR_2g097473 22.905 358 251 9 137 484 311 653 6.06e-23 102
MS.gene20860.t1 MTR_5g094450 24.745 392 264 14 109 485 157 532 1.69e-22 100
MS.gene20860.t1 MTR_2g097467 22.841 359 250 8 137 484 311 653 1.85e-22 101
MS.gene20860.t1 MTR_2g097380 24.202 376 232 15 118 484 229 560 1.88e-22 100
MS.gene20860.t1 MTR_2g082090 25.210 357 235 13 142 484 240 578 2.48e-22 100
MS.gene20860.t1 MTR_1g069725 26.950 282 196 8 206 484 188 462 6.37e-22 98.6
MS.gene20860.t1 MTR_2g097350 24.051 395 258 13 108 484 269 639 4.32e-21 97.1
MS.gene20860.t1 MTR_3g022830 24.685 397 228 11 146 486 56 437 2.14e-20 94.0
MS.gene20860.t1 MTR_5g097480 23.143 350 246 10 146 485 208 544 3.67e-20 94.0
MS.gene20860.t1 MTR_4g076020 23.577 369 253 9 118 485 89 429 4.55e-20 92.8
MS.gene20860.t1 MTR_4g076140 32.919 161 100 4 329 485 313 469 7.07e-20 92.4
MS.gene20860.t1 MTR_3g089055 27.526 287 186 9 213 485 292 570 1.90e-19 91.7
MS.gene20860.t1 MTR_3g089055 27.526 287 186 9 213 485 287 565 2.25e-19 91.7
MS.gene20860.t1 MTR_4g077760 22.917 384 254 14 118 485 198 555 9.61e-19 89.4
MS.gene20860.t1 MTR_1g029420 27.431 288 183 11 214 485 315 592 1.00e-18 89.7
MS.gene20860.t1 MTR_3g072710 22.829 403 277 12 103 484 110 499 1.68e-17 85.5
MS.gene20860.t1 MTR_5g015490 23.416 363 255 9 138 485 136 490 7.70e-17 83.2
MS.gene20860.t1 MTR_7g057230 23.684 380 267 11 112 484 293 656 1.35e-16 83.2
MS.gene20860.t1 MTR_5g058860 21.975 405 278 11 102 484 108 496 7.47e-15 77.4
MS.gene20860.t1 MTR_4g026485 24.912 285 196 8 210 485 349 624 9.33e-15 77.4
MS.gene20860.t1 MTR_8g093070 25.195 385 236 17 121 484 142 495 3.44e-14 75.1
MS.gene20860.t1 MTR_1g096030 22.695 423 268 18 98 487 65 461 3.92e-12 68.6
MS.gene20860.t1 MTR_2g099110 20.548 365 264 9 138 485 96 451 3.93e-11 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene20860.t1 AT1G66350 25.974 385 262 7 102 485 144 506 1.67e-42 158
MS.gene20860.t1 AT3G03450 27.068 399 259 8 99 486 169 546 3.63e-42 158
MS.gene20860.t1 AT2G01570 25.954 393 266 8 99 486 210 582 4.20e-41 155
MS.gene20860.t1 AT5G17490 27.436 390 253 9 104 486 151 517 2.59e-39 149
MS.gene20860.t1 AT1G14920 26.768 396 259 9 99 486 158 530 2.64e-39 149
MS.gene20860.t1 AT3G46600 26.750 400 243 14 105 485 3 371 9.60e-35 134
MS.gene20860.t1 AT2G29065 26.703 367 239 9 136 484 273 627 1.10e-34 137
MS.gene20860.t1 AT5G59450 27.250 400 246 14 108 485 222 598 2.11e-34 136
MS.gene20860.t1 AT3G46600 26.799 403 245 14 102 485 176 547 2.27e-34 136
MS.gene20860.t1 AT3G46600 26.799 403 245 14 102 485 208 579 3.71e-34 135
MS.gene20860.t1 AT3G54220 27.273 374 243 13 118 484 298 649 1.25e-29 123
MS.gene20860.t1 AT1G63100 26.510 298 201 7 202 484 359 653 3.89e-29 121
MS.gene20860.t1 AT1G63100 26.510 298 201 7 202 484 334 628 3.92e-29 121
MS.gene20860.t1 AT1G07530 24.505 404 262 11 101 484 384 764 1.11e-28 120
MS.gene20860.t1 AT4G17230 24.937 397 261 13 105 485 150 525 1.65e-28 119
MS.gene20860.t1 AT4G17230 24.937 397 261 13 105 485 150 525 1.65e-28 119
MS.gene20860.t1 AT5G41920 26.221 389 266 12 100 485 30 400 7.36e-28 115
MS.gene20860.t1 AT1G50420 24.889 450 252 15 104 485 48 479 8.08e-27 113
MS.gene20860.t1 AT2G29060 25.990 404 242 14 109 484 317 691 1.28e-26 114
MS.gene20860.t1 AT2G29060 25.990 404 242 14 109 484 280 654 1.43e-26 114
MS.gene20860.t1 AT1G07520 24.100 361 237 9 145 484 184 528 3.54e-26 112
MS.gene20860.t1 AT1G07520 24.100 361 237 9 145 484 184 528 3.54e-26 112
MS.gene20860.t1 AT1G07520 24.586 362 234 10 145 484 348 692 3.62e-26 112
MS.gene20860.t1 AT1G07520 24.586 362 234 10 145 484 349 693 3.72e-26 112
MS.gene20860.t1 AT1G50600 25.496 353 234 11 146 485 190 526 2.78e-24 106
MS.gene20860.t1 AT1G50600 25.496 353 234 11 146 485 265 601 3.04e-24 106
MS.gene20860.t1 AT1G50600 25.496 353 234 11 146 485 261 597 3.95e-24 106
MS.gene20860.t1 AT1G55580 25.668 374 241 11 146 485 75 445 1.87e-23 103
MS.gene20860.t1 AT2G37650 24.679 389 265 9 108 485 342 713 2.81e-23 103
MS.gene20860.t1 AT2G37650 24.679 389 265 9 108 485 330 701 2.95e-23 103
MS.gene20860.t1 AT2G04890 25.199 377 252 12 121 485 55 413 1.44e-22 100
MS.gene20860.t1 AT5G48150 23.760 383 264 11 118 485 121 490 5.08e-22 99.4
MS.gene20860.t1 AT5G48150 23.760 383 264 11 118 485 121 490 5.08e-22 99.4
MS.gene20860.t1 AT5G48150 23.760 383 264 11 118 485 121 490 5.08e-22 99.4
MS.gene20860.t1 AT5G48150 23.760 383 264 11 118 485 121 490 5.08e-22 99.4
MS.gene20860.t1 AT4G37650 22.821 390 276 8 114 485 147 529 9.67e-21 95.5
MS.gene20860.t1 AT5G66770 22.778 360 248 11 139 485 242 584 1.19e-17 86.3
MS.gene20860.t1 AT3G50650 22.526 293 213 7 206 485 251 542 3.90e-15 78.2
MS.gene20860.t1 AT3G49950 24.749 299 199 8 206 484 115 407 1.75e-14 75.9
MS.gene20860.t1 AT4G08250 24.641 418 272 18 87 484 82 476 9.57e-14 73.9
MS.gene20860.t1 AT4G36710 22.946 353 237 15 146 486 157 486 1.83e-11 66.6
MS.gene20860.t1 AT1G21450 23.183 289 197 9 213 485 314 593 2.32e-11 66.6

Find 67 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATGGTAAGTGTTTAATAAT+TGG 0.167586 1.3:-61549150 MS.gene20860:CDS
CATTTGATTCATCTTAATAT+TGG 0.236657 1.3:-61549855 MS.gene20860:CDS
GACGGAATTGATTGATAAAA+TGG 0.247556 1.3:-61549991 MS.gene20860:CDS
AATAATCTTTACATTCCTTA+TGG 0.249550 1.3:-61550287 MS.gene20860:CDS
CATGCCACAGTGATGAGTTT+TGG 0.263501 1.3:+61550312 None:intergenic
ATTCCTTATGGAACTGTTCT+TGG 0.265945 1.3:-61550275 MS.gene20860:CDS
TCGCAAACAAGGGTGAATTA+TGG 0.302490 1.3:+61549469 None:intergenic
AAGGCTAAGTTAGAAGAAAT+TGG 0.303167 1.3:-61549735 MS.gene20860:CDS
TCCATGTTCACTTAGGACTA+TGG 0.336208 1.3:-61549580 MS.gene20860:CDS
GATAAGAAACATTGTTGGTT+TGG 0.346018 1.3:-61549334 MS.gene20860:CDS
ATGAATGAAAGTGTAAATAT+TGG 0.346851 1.3:-61550128 MS.gene20860:CDS
ATTCTGAAGATCACAGCTAT+TGG 0.354564 1.3:-61549768 MS.gene20860:CDS
AATCAAGTAATGCAATTTGC+TGG 0.354696 1.3:-61549921 MS.gene20860:CDS
TTATGAGTGTAGAGTGATAA+TGG 0.355610 1.3:-61549376 MS.gene20860:CDS
GAAGATATAATAACACTTGC+AGG 0.358002 1.3:-61550347 MS.gene20860:CDS
ATTCACCCTTGTTTGCGAAT+AGG 0.360715 1.3:-61549464 MS.gene20860:CDS
CTTGAAGTGCTTGAGAGAAA+TGG 0.363368 1.3:+61550081 None:intergenic
CAAGCTGAGACTGAATGAAT+TGG 0.383878 1.3:+61549114 None:intergenic
AGGTATAGAATGGTAGAAAC+TGG 0.390006 1.3:-61549252 MS.gene20860:CDS
GGAATGTGAAGATTGATGTT+TGG 0.392726 1.3:-61549290 MS.gene20860:CDS
TCAAGTACTATCATAATAAA+CGG 0.431397 1.3:+61549504 None:intergenic
CGGACGAGGAAAGATGAAAC+TGG 0.435732 1.3:-61550039 MS.gene20860:CDS
CAGTTTCATCTTTCCTCGTC+CGG 0.439195 1.3:+61550040 None:intergenic
GAGAGAATCAACAGAGAAAC+CGG 0.442776 1.3:-61550059 MS.gene20860:CDS
CAATATTAAGATGAATCAAA+TGG 0.442889 1.3:+61549856 None:intergenic
AATTGCATTACTTGATTGAA+AGG 0.446453 1.3:+61549927 None:intergenic
ATTCATCTTAATATTGGATG+TGG 0.448243 1.3:-61549849 MS.gene20860:CDS
TTTATTCTCCATGTTCACTT+AGG 0.457172 1.3:-61549587 MS.gene20860:CDS
TGTTGATACTTGTATAAAAG+AGG 0.464976 1.3:-61549400 MS.gene20860:CDS
GGTATCAAATTCTGATGGCT+TGG 0.468081 1.3:-61549559 MS.gene20860:CDS
GTGATCTTCAGAATTTCAAC+CGG 0.468897 1.3:+61549777 None:intergenic
ATCAATTCCACGGCTTAAGC+AGG 0.469908 1.3:-61550495 MS.gene20860:CDS
TGCTGGTGTACAAGCTATAG+TGG 0.474092 1.3:-61549904 MS.gene20860:CDS
GAAGGGATAAGAAACATTGT+TGG 0.474796 1.3:-61549339 MS.gene20860:CDS
TGTTCTTGGAAGCTTGTTGT+CGG 0.475836 1.3:-61550261 MS.gene20860:CDS
GTTCTTTGCAAGGTATAGAA+TGG 0.484270 1.3:-61549262 MS.gene20860:CDS
AAGATGAAGAAAGGAAGGTT+AGG 0.502253 1.3:-61549311 MS.gene20860:CDS
TGTTTAATAATTGGATGGAA+GGG 0.503361 1.3:-61549141 MS.gene20860:CDS
CAATTCATTCAGTCTCAGCT+TGG 0.509421 1.3:-61549113 MS.gene20860:CDS
TTGGTTTGGAAGATGAAGAA+AGG 0.515913 1.3:-61549320 MS.gene20860:CDS
GTGTTTAATAATTGGATGGA+AGG 0.523650 1.3:-61549142 MS.gene20860:CDS
GTTTAATAATTGGATGGAAG+GGG 0.527241 1.3:-61549140 MS.gene20860:CDS
TTTGGAAGATGAAGAAAGGA+AGG 0.529462 1.3:-61549316 MS.gene20860:CDS
CTTCCAAGAACAGTTCCATA+AGG 0.531300 1.3:+61550272 None:intergenic
AACAAACCTATTCGCAAACA+AGG 0.532041 1.3:+61549458 None:intergenic
GTAAGTGTTTAATAATTGGA+TGG 0.532866 1.3:-61549146 MS.gene20860:CDS
AAGCCGTGGAATTGATATCA+TGG 0.533713 1.3:+61550502 None:intergenic
TCAAGTGATGCTAAAGAAGA+TGG 0.542006 1.3:+61550458 None:intergenic
TTTGGAGAAGGTTCTTTGCA+AGG 0.543910 1.3:-61549272 MS.gene20860:CDS
ATGGAAGCAATATTATCTGA+AGG 0.550756 1.3:-61549357 MS.gene20860:CDS
AGTAGACCGCAAGGAAAAGC+CGG 0.553680 1.3:-61549796 MS.gene20860:CDS
CTAGCAGCAGCTGAGAGAGT+AGG 0.586422 1.3:-61550200 MS.gene20860:CDS
ACCATAGTCCTAAGTGAACA+TGG 0.589256 1.3:+61549579 None:intergenic
TGGAAGCAATATTATCTGAA+GGG 0.593391 1.3:-61549356 MS.gene20860:CDS
TGAAGATTGATGTTTGGAGA+AGG 0.596614 1.3:-61549284 MS.gene20860:CDS
TGGCTTGGAGAATTTGATGA+AGG 0.603211 1.3:-61549544 MS.gene20860:CDS
ACTATGGTATCAAATTCTGA+TGG 0.608580 1.3:-61549564 MS.gene20860:CDS
AAATGAGAGAAACAAGGAGA+CGG 0.612044 1.3:-61550009 MS.gene20860:CDS
ATCAACAGAGAAACCGGACG+AGG 0.613864 1.3:-61550053 MS.gene20860:CDS
AAGCCATGATATCAATTCCA+CGG 0.616214 1.3:-61550505 MS.gene20860:CDS
ATTTGCACCTGCTTAAGCCG+TGG 0.626082 1.3:+61550488 None:intergenic
GCAAGCATTAGTAGACCGCA+AGG 0.627501 1.3:-61549805 MS.gene20860:CDS
ACAAACCTATTCGCAAACAA+GGG 0.673185 1.3:+61549459 None:intergenic
TAGATCAAATGAGAGAAACA+AGG 0.680410 1.3:-61550015 MS.gene20860:CDS
GATGCTAAAGAAGATGGTGG+TGG 0.682167 1.3:+61550464 None:intergenic
AGTGATGCTAAAGAAGATGG+TGG 0.725977 1.3:+61550461 None:intergenic
ATATCCAAAACTCATCACTG+TGG 0.776374 1.3:-61550316 MS.gene20860:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAACTATGTTATATAAAA+AGG + chr1.3:61549972-61549991 None:intergenic 10.0%
!! AAAACTATGTTATATAAAAA+GGG + chr1.3:61549971-61549990 None:intergenic 10.0%
!! AATAATCTTTACATTCCTTA+TGG - chr1.3:61549356-61549375 MS.gene20860:CDS 20.0%
!! AATGGTAAGTGTTTAATAAT+TGG - chr1.3:61550493-61550512 MS.gene20860:CDS 20.0%
!! CAATATTAAGATGAATCAAA+TGG + chr1.3:61549790-61549809 None:intergenic 20.0%
!! CATTTGATTCATCTTAATAT+TGG - chr1.3:61549788-61549807 MS.gene20860:CDS 20.0%
!! TCAAGTACTATCATAATAAA+CGG + chr1.3:61550142-61550161 None:intergenic 20.0%
!!! ATGAATGAAAGTGTAAATAT+TGG - chr1.3:61549515-61549534 MS.gene20860:CDS 20.0%
!!! TTTTGTACTATAGACAAAAA+TGG - chr1.3:61550475-61550494 MS.gene20860:CDS 20.0%
! AATTGCATTACTTGATTGAA+AGG + chr1.3:61549719-61549738 None:intergenic 25.0%
! ATTCATCTTAATATTGGATG+TGG - chr1.3:61549794-61549813 MS.gene20860:CDS 25.0%
! CTTGAGCTATTGAAAAATTA+TGG - chr1.3:61549203-61549222 MS.gene20860:CDS 25.0%
! GTAAGTGTTTAATAATTGGA+TGG - chr1.3:61550497-61550516 MS.gene20860:CDS 25.0%
! TGTTGATACTTGTATAAAAG+AGG - chr1.3:61550243-61550262 MS.gene20860:CDS 25.0%
! TGTTTAATAATTGGATGGAA+GGG - chr1.3:61550502-61550521 MS.gene20860:CDS 25.0%
! TTACTAAAAAGTTTGACTGT+GGG - chr1.3:61550449-61550468 MS.gene20860:CDS 25.0%
!! ATATAAAAAGGGTATGTTCA+AGG + chr1.3:61549960-61549979 None:intergenic 25.0%
AAGGCTAAGTTAGAAGAAAT+TGG - chr1.3:61549908-61549927 MS.gene20860:CDS 30.0%
AATCAAGTAATGCAATTTGC+TGG - chr1.3:61549722-61549741 MS.gene20860:CDS 30.0%
ACTATGGTATCAAATTCTGA+TGG - chr1.3:61550079-61550098 MS.gene20860:CDS 30.0%
ATGGAAGCAATATTATCTGA+AGG - chr1.3:61550286-61550305 MS.gene20860:CDS 30.0%
GAAGATATAATAACACTTGC+AGG - chr1.3:61549296-61549315 MS.gene20860:CDS 30.0%
GACGGAATTGATTGATAAAA+TGG - chr1.3:61549652-61549671 MS.gene20860:CDS 30.0%
GTGTTTAATAATTGGATGGA+AGG - chr1.3:61550501-61550520 MS.gene20860:CDS 30.0%
GTTACTAAAAAGTTTGACTG+TGG - chr1.3:61550448-61550467 MS.gene20860:CDS 30.0%
GTTTAATAATTGGATGGAAG+GGG - chr1.3:61550503-61550522 MS.gene20860:CDS 30.0%
TAGATCAAATGAGAGAAACA+AGG - chr1.3:61549628-61549647 MS.gene20860:CDS 30.0%
TGGAAGCAATATTATCTGAA+GGG - chr1.3:61550287-61550306 MS.gene20860:CDS 30.0%
TTATGAGTGTAGAGTGATAA+TGG - chr1.3:61550267-61550286 MS.gene20860:CDS 30.0%
TTTATTCTCCATGTTCACTT+AGG - chr1.3:61550056-61550075 MS.gene20860:CDS 30.0%
! AGCTATTGGATTTTCTAACA+AGG - chr1.3:61549889-61549908 MS.gene20860:CDS 30.0%
! AGTTTTTGTTGAAGATGCTA+TGG - chr1.3:61549985-61550004 MS.gene20860:CDS 30.0%
! GATAAGAAACATTGTTGGTT+TGG - chr1.3:61550309-61550328 MS.gene20860:CDS 30.0%
AAATGAGAGAAACAAGGAGA+CGG - chr1.3:61549634-61549653 MS.gene20860:CDS 35.0%
AACAAACCTATTCGCAAACA+AGG + chr1.3:61550188-61550207 None:intergenic 35.0%
AAGATGAAGAAAGGAAGGTT+AGG - chr1.3:61550332-61550351 MS.gene20860:CDS 35.0%
AAGCCATGATATCAATTCCA+CGG - chr1.3:61549138-61549157 MS.gene20860:CDS 35.0%
ACAAACCTATTCGCAAACAA+GGG + chr1.3:61550187-61550206 None:intergenic 35.0%
AGGTATAGAATGGTAGAAAC+TGG - chr1.3:61550391-61550410 MS.gene20860:CDS 35.0%
ATATCCAAAACTCATCACTG+TGG - chr1.3:61549327-61549346 MS.gene20860:CDS 35.0%
ATTCCTTATGGAACTGTTCT+TGG - chr1.3:61549368-61549387 MS.gene20860:CDS 35.0%
GAAGGGATAAGAAACATTGT+TGG - chr1.3:61550304-61550323 MS.gene20860:CDS 35.0%
GGAATGTGAAGATTGATGTT+TGG - chr1.3:61550353-61550372 MS.gene20860:CDS 35.0%
GTGATCTTCAGAATTTCAAC+CGG + chr1.3:61549869-61549888 None:intergenic 35.0%
GTTCTTTGCAAGGTATAGAA+TGG - chr1.3:61550381-61550400 MS.gene20860:CDS 35.0%
TCAAGTGATGCTAAAGAAGA+TGG + chr1.3:61549188-61549207 None:intergenic 35.0%
TGAAGATTGATGTTTGGAGA+AGG - chr1.3:61550359-61550378 MS.gene20860:CDS 35.0%
TTTGGAAGATGAAGAAAGGA+AGG - chr1.3:61550327-61550346 MS.gene20860:CDS 35.0%
! ATTCTGAAGATCACAGCTAT+TGG - chr1.3:61549875-61549894 MS.gene20860:CDS 35.0%
! TCTTCAGTTGACAACTTTTG+TGG + chr1.3:61549281-61549300 None:intergenic 35.0%
!! TTGGTTTGGAAGATGAAGAA+AGG - chr1.3:61550323-61550342 MS.gene20860:CDS 35.0%
AAGCCGTGGAATTGATATCA+TGG + chr1.3:61549144-61549163 None:intergenic 40.0%
ACCATAGTCCTAAGTGAACA+TGG + chr1.3:61550067-61550086 None:intergenic 40.0%
AGTGATGCTAAAGAAGATGG+TGG + chr1.3:61549185-61549204 None:intergenic 40.0%
CAAGCTGAGACTGAATGAAT+TGG + chr1.3:61550532-61550551 None:intergenic 40.0%
CAATTCATTCAGTCTCAGCT+TGG - chr1.3:61550530-61550549 MS.gene20860:CDS 40.0%
CTTCCAAGAACAGTTCCATA+AGG + chr1.3:61549374-61549393 None:intergenic 40.0%
GAGAGAATCAACAGAGAAAC+CGG - chr1.3:61549584-61549603 MS.gene20860:CDS 40.0%
GGTATCAAATTCTGATGGCT+TGG - chr1.3:61550084-61550103 MS.gene20860:CDS 40.0%
TCCATGTTCACTTAGGACTA+TGG - chr1.3:61550063-61550082 MS.gene20860:CDS 40.0%
TCGCAAACAAGGGTGAATTA+TGG + chr1.3:61550177-61550196 None:intergenic 40.0%
TTTGGAGAAGGTTCTTTGCA+AGG - chr1.3:61550371-61550390 MS.gene20860:CDS 40.0%
! ATTCACCCTTGTTTGCGAAT+AGG - chr1.3:61550179-61550198 MS.gene20860:CDS 40.0%
! CTTGAAGTGCTTGAGAGAAA+TGG + chr1.3:61549565-61549584 None:intergenic 40.0%
! TGGCTTGGAGAATTTGATGA+AGG - chr1.3:61550099-61550118 MS.gene20860:CDS 40.0%
!! TGTTCTTGGAAGCTTGTTGT+CGG - chr1.3:61549382-61549401 MS.gene20860:CDS 40.0%
ATCAATTCCACGGCTTAAGC+AGG - chr1.3:61549148-61549167 MS.gene20860:CDS 45.0%
CAGTTTCATCTTTCCTCGTC+CGG + chr1.3:61549606-61549625 None:intergenic 45.0%
TGCTGGTGTACAAGCTATAG+TGG - chr1.3:61549739-61549758 MS.gene20860:CDS 45.0%
! CATGCCACAGTGATGAGTTT+TGG + chr1.3:61549334-61549353 None:intergenic 45.0%
! GATGCTAAAGAAGATGGTGG+TGG + chr1.3:61549182-61549201 None:intergenic 45.0%
!! TTTCAACCGGCTTTTCCTTG+CGG + chr1.3:61549856-61549875 None:intergenic 45.0%
AGTAGACCGCAAGGAAAAGC+CGG - chr1.3:61549847-61549866 MS.gene20860:CDS 50.0%
ATCAACAGAGAAACCGGACG+AGG - chr1.3:61549590-61549609 MS.gene20860:CDS 50.0%
ATTTGCACCTGCTTAAGCCG+TGG + chr1.3:61549158-61549177 None:intergenic 50.0%
CGGACGAGGAAAGATGAAAC+TGG - chr1.3:61549604-61549623 MS.gene20860:CDS 50.0%
GCAAGCATTAGTAGACCGCA+AGG - chr1.3:61549838-61549857 MS.gene20860:CDS 50.0%
CTAGCAGCAGCTGAGAGAGT+AGG - chr1.3:61549443-61549462 MS.gene20860:CDS 55.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 61549101 61550564 61549101 ID=MS.gene20860
chr1.3 mRNA 61549101 61550564 61549101 ID=MS.gene20860.t1;Parent=MS.gene20860
chr1.3 exon 61549101 61550564 61549101 ID=MS.gene20860.t1.exon1;Parent=MS.gene20860.t1
chr1.3 CDS 61549101 61550564 61549101 ID=cds.MS.gene20860.t1;Parent=MS.gene20860.t1
Gene Sequence

>MS.gene20860

ATGGAGAAGTTACTACAAGATCTAGAGCAAGAAAATCAAGCCATGATATCAATTCCACGGCTTAAGCAGGTGCAAATGCCACCACCATCTTCTTTAGCATCACTTGAGCTATTGAAAAATTATGGAAGCAAGTTCAATAAGCAAAAAAGCAAAAGCAATGTTGAAACTTGTGTAAGTCCACAAAAGTTGTCAACTGAAGATATAATAACACTTGCAGGAACGAAGTATATCCAAAACTCATCACTGTGGCATGATAATAATCTTTACATTCCTTATGGAACTGTTCTTGGAAGCTTGTTGTCGGACGAAGAAAATAAACAAGTTGAACTAGCTCAGTTTGTTCTAGCAGCAGCTGAGAGAGTAGGTTGTCAACAATTTGAACGTGCAAACATGTTACTTTCACATTTTCATCTCATGAATGAAAGTGTAAATATTGGAAGCACAGTTCAGAGACTTCTTTTCCATTTCTCTCAAGCACTTCAAGAGAGAATCAACAGAGAAACCGGACGAGGAAAGATGAAACTGGATAGATCAAATGAGAGAAACAAGGAGACGGAATTGATTGATAAAATGGAATTAGATACTAACATAGTAGTTAAATGTCATCAAAAAATTCCTTTCAATCAAGTAATGCAATTTGCTGGTGTACAAGCTATAGTGGAACATGTTGCATCACAAACAAAAATCCATTTGATTCATCTTAATATTGGATGTGGAGTGATGAGTACATGTTTCATGCAAGCATTAGTAGACCGCAAGGAAAAGCCGGTTGAAATTCTGAAGATCACAGCTATTGGATTTTCTAACAAGGCTAAGTTAGAAGAAATTGGCAAAAGTTTAATGAGTTTTGCTGAATCCTTGAACATACCCTTTTTATATAACATAGTTTTTGTTGAAGATGCTATGGAGATTAAGTTAGATCAATTTGAAATTGAATATGATGAAGCTGTTGCTATTTATTCTCCATGTTCACTTAGGACTATGGTATCAAATTCTGATGGCTTGGAGAATTTGATGAAGGTGATAAGAAAAATGAAACCGTTTATTATGATAGTACTTGAACTTGAAGCAAACCATAATTCACCCTTGTTTGCGAATAGGTTTGTTGAAGCATTGTTCTTTTACTCAGCATTTTTTGACTGTGTTGATACTTGTATAAAAGAGGATTATGAGTGTAGAGTGATAATGGAAGCAATATTATCTGAAGGGATAAGAAACATTGTTGGTTTGGAAGATGAAGAAAGGAAGGTTAGGAATGTGAAGATTGATGTTTGGAGAAGGTTCTTTGCAAGGTATAGAATGGTAGAAACTGGATTCAGTGAGTCTTCAATTTATCAAGCTAATTTAGTTACTAAAAAGTTTGACTGTGGGAAGTTTTGTACTATAGACAAAAATGGTAAGTGTTTAATAATTGGATGGAAGGGGACTCCAATTCATTCAGTCTCAGCTTGGAAGTTTCTTTGA
Protein sequence

>MS.gene20860.t1

MEKLLQDLEQENQAMISIPRLKQVQMPPPSSLASLELLKNYGSKFNKQKSKSNVETCVSPQKLSTEDIITLAGTKYIQNSSLWHDNNLYIPYGTVLGSLLSDEENKQVELAQFVLAAAERVGCQQFERANMLLSHFHLMNESVNIGSTVQRLLFHFSQALQERINRETGRGKMKLDRSNERNKETELIDKMELDTNIVVKCHQKIPFNQVMQFAGVQAIVEHVASQTKIHLIHLNIGCGVMSTCFMQALVDRKEKPVEILKITAIGFSNKAKLEEIGKSLMSFAESLNIPFLYNIVFVEDAMEIKLDQFEIEYDEAVAIYSPCSLRTMVSNSDGLENLMKVIRKMKPFIMIVLELEANHNSPLFANRFVEALFFYSAFFDCVDTCIKEDYECRVIMEAILSEGIRNIVGLEDEERKVRNVKIDVWRRFFARYRMVETGFSESSIYQANLVTKKFDCGKFCTIDKNGKCLIIGWKGTPIHSVSAWKFL