AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21012

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21012.t1 MTR_1g084150 76.824 233 27 2 145 350 1 233 4.87e-109 318
MS.gene21012.t1 MTR_5g095070 58.923 297 92 7 2 298 5 271 1.69e-104 308
MS.gene21012.t1 MTR_5g080030 62.609 230 79 1 32 261 44 266 6.55e-95 288
MS.gene21012.t1 MTR_5g080030 53.254 169 58 6 183 350 264 412 4.68e-38 140
MS.gene21012.t1 MTR_4g015240 51.220 82 19 2 259 339 39 100 7.70e-17 75.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 73 sgRNAs with CRISPR-Local

Find 151 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTCGTTGGATATGTTAAA+TGG 0.182684 1.3:+59893335 MS.gene21012:CDS
TACTGGTTTATCTCGATTAA+TGG 0.250811 1.3:+59892292 MS.gene21012:CDS
CCTTCGGTGCTGCCGGTTAC+AGG 0.272596 1.3:-59891911 None:intergenic
AGTTGTCTCTGCACTGAAAT+TGG 0.295292 1.3:+59891799 MS.gene21012:CDS
AACAGTACCATTGGTGAGTT+TGG 0.303944 1.3:-59890708 None:intergenic
TGTCAAACTAACTGAAGTTT+TGG 0.307954 1.3:+59890766 MS.gene21012:CDS
TCCCTTCTTCCCCGTTGATT+TGG 0.311486 1.3:+59893304 MS.gene21012:CDS
TTCTTGCTTTCCTCCACATA+TGG 0.365771 1.3:-59893053 None:intergenic
TTTGTAAAGGTACGCGTAAA+AGG 0.366190 1.3:+59892255 MS.gene21012:intron
TTCAGTTAGTTTGACATAGA+AGG 0.372838 1.3:-59890759 None:intergenic
TTCTACAGGTTTCCTGTAAC+CGG 0.383374 1.3:+59891899 MS.gene21012:CDS
GAAGAGCACTCAAGATCTTA+AGG 0.392322 1.3:+59893154 MS.gene21012:CDS
AGGTTGGTCTTCAAAACAAA+TGG 0.395740 1.3:+59891771 MS.gene21012:intron
TTGAGAAATTGTACTTCTAC+AGG 0.399495 1.3:+59891885 MS.gene21012:CDS
AATCCTTATAACCTGTCATT+TGG 0.408344 1.3:-59892364 None:intergenic
CAACCGCTAGTATAGTCATT+TGG 0.413628 1.3:-59893363 None:intergenic
CTCTATCTCTAAGTCATCAT+TGG 0.415656 1.3:-59890735 None:intergenic
CAGTAAGTATCCAAATCAAC+GGG 0.418388 1.3:-59893314 None:intergenic
GGAGGAAAGCAAGAAGATAA+AGG 0.418994 1.3:+59893061 MS.gene21012:CDS
TGGTGACTTCCAAGTAAAAC+AGG 0.431064 1.3:-59891474 None:intergenic
AATGGAAGGCAACAAGACTA+TGG 0.438680 1.3:+59890661 None:intergenic
CTGGTGAAAACCTCCTCTTC+TGG 0.440306 1.3:-59891494 None:intergenic
ATCCAAATCAACGGGGAAGA+AGG 0.448267 1.3:-59893306 None:intergenic
GCTCATGATCCGGACATTGA+TGG 0.456058 1.3:+59892853 MS.gene21012:CDS
GTTCGTTGGATATGTTAAAT+GGG 0.459203 1.3:+59893336 MS.gene21012:CDS
TACGCATGGCTGTTGATGCT+TGG 0.460942 1.3:+59892887 MS.gene21012:CDS
AGCCAGCTGTCAAGAACCTT+GGG 0.462711 1.3:+59892699 MS.gene21012:CDS
AAGACAAAGAAAAGAAGAAA+CGG 0.466286 1.3:+59892764 MS.gene21012:CDS
TATTTGATATCCACTTTGTC+CGG 0.466627 1.3:-59892800 None:intergenic
CACCCAAGGTTCTTGACAGC+TGG 0.474988 1.3:-59892701 None:intergenic
TTTGGATACTTACTGTTCGT+TGG 0.489477 1.3:+59893322 MS.gene21012:CDS
TTAGATACCTTCGGTGCTGC+CGG 0.491330 1.3:-59891918 None:intergenic
ACAGAAGAATAGTTTGGTAG+TGG 0.492096 1.3:-59893227 None:intergenic
AGGAAGCAGAACTCGACTAC+TGG 0.510036 1.3:+59892275 MS.gene21012:CDS
GATGTCTGCCACTGATAAAG+AGG 0.517133 1.3:+59892915 MS.gene21012:CDS
GAGCCAGCTGTCAAGAACCT+TGG 0.520532 1.3:+59892698 MS.gene21012:CDS
CAACAATGCAGCTGTGGAGT+TGG 0.523389 1.3:+59893250 MS.gene21012:CDS
TTGTTGACAGAAGAATAGTT+TGG 0.526138 1.3:-59893233 None:intergenic
GAATGTGGCAAAGGTGTCCA+AGG 0.526713 1.3:+59892331 MS.gene21012:CDS
TTCTGTCAACAATGCAGCTG+TGG 0.527890 1.3:+59893244 MS.gene21012:CDS
TCCAAATCAACGGGGAAGAA+GGG 0.540234 1.3:-59893305 None:intergenic
TACTTGGAAGTCACCAGAAG+AGG 0.540823 1.3:+59891481 MS.gene21012:CDS
GGTTGGTCTTCAAAACAAAT+GGG 0.543380 1.3:+59891772 MS.gene21012:intron
TCGATTAAAAGCTCATGATC+CGG 0.546735 1.3:+59892843 MS.gene21012:CDS
GAGCACTGAAGATCTTAAGA+AGG 0.550700 1.3:+59893124 MS.gene21012:CDS
TGATATCCACTTTGTCCGGT+AGG 0.555314 1.3:-59892796 None:intergenic
GGCTACTCTAGCCAAATGAC+AGG 0.557780 1.3:+59892353 MS.gene21012:CDS
CCTGTAACCGGCAGCACCGA+AGG 0.559034 1.3:+59891911 MS.gene21012:CDS
CATTTGGCTAGAGTAGCCCT+TGG 0.560486 1.3:-59892348 None:intergenic
ATTGGACACAACAGTACCAT+TGG 0.564594 1.3:-59890717 None:intergenic
GTCTCTGCACTGAAATTGGA+AGG 0.566571 1.3:+59891803 MS.gene21012:CDS
AAGATCTTAAGGAGGAAAAG+AGG 0.571062 1.3:+59893165 MS.gene21012:CDS
AAACGGCGAGGTCCTCCTAC+CGG 0.572639 1.3:+59892781 MS.gene21012:CDS
TTCCTAGCTCAAACAATGGA+AGG 0.574513 1.3:+59890647 None:intergenic
TAGTCGTAACCGCACACACT+AGG 0.575038 1.3:-59893188 None:intergenic
TTGGAAGTCACCAGAAGAGG+AGG 0.590853 1.3:+59891484 MS.gene21012:CDS
GTATCTGAAGGTGATGATGA+TGG 0.592975 1.3:-59890686 None:intergenic
TATCCACTTTGTCCGGTAGG+AGG 0.594215 1.3:-59892793 None:intergenic
ACAGTAAGTATCCAAATCAA+CGG 0.594862 1.3:-59893315 None:intergenic
TTATCATACCTCTTTATCAG+TGG 0.622643 1.3:-59892923 None:intergenic
GGTCCTCCTACCGGACAAAG+TGG 0.623840 1.3:+59892790 MS.gene21012:CDS
GGTGCTTGTAGAAGCACCCA+AGG 0.627728 1.3:-59892715 None:intergenic
ACAAGTCTAAGATTGTCTCT+CGG 0.631739 1.3:-59891450 None:intergenic
GAAAAGAGGCCTAGTGTGTG+CGG 0.639000 1.3:+59893179 MS.gene21012:CDS
TCAGATACCAAACTCACCAA+TGG 0.649186 1.3:+59890701 MS.gene21012:CDS
AGATAATCTGAATGTGGCAA+AGG 0.661885 1.3:+59892322 MS.gene21012:CDS
GAGCACTCAAGATCTTAAGG+AGG 0.665195 1.3:+59893157 MS.gene21012:CDS
AATGTGGCAAAGGTGTCCAA+GGG 0.667130 1.3:+59892332 MS.gene21012:CDS
TTGGTGAGTTTGGTATCTGA+AGG 0.672081 1.3:-59890698 None:intergenic
TCATGAAGATAATCTGAATG+TGG 0.680646 1.3:+59892316 MS.gene21012:CDS
AAAGAAAAGAAGAAACGGCG+AGG 0.681943 1.3:+59892769 MS.gene21012:CDS
AGACATGAGATTGAACCCAG+AGG 0.774697 1.3:-59890792 None:intergenic
AGTAAGTATCCAAATCAACG+GGG 0.799229 1.3:-59893313 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATAAAAAAGTAATTATAAA+TGG - chr1.3:59893030-59893049 None:intergenic 10.0%
!! ATTTGGATAAAAAAAAATGA+TGG + chr1.3:59891368-59891387 MS.gene21012:intron 15.0%
!!! AAAAAAATGATGGATAATAA+AGG + chr1.3:59891378-59891397 MS.gene21012:intron 15.0%
!!! AAAAAATGATGGATAATAAA+GGG + chr1.3:59891379-59891398 MS.gene21012:intron 15.0%
!!! TAAAGTTTTATGAGATTATT+TGG + chr1.3:59891351-59891370 MS.gene21012:intron 15.0%
!!! TTTTATTACGTTTTTGTTTT+AGG + chr1.3:59892176-59892195 MS.gene21012:intron 15.0%
!! TAATACAAATTAGTCAATAG+AGG + chr1.3:59891676-59891695 MS.gene21012:intron 20.0%
!! TTTATTCCTCTTTAAATGTT+TGG + chr1.3:59891264-59891283 MS.gene21012:intron 20.0%
!!! ATAAAAATATTTAGTTCGAG+GGG + chr1.3:59891118-59891137 MS.gene21012:intron 20.0%
!!! GATAAAAATATTTAGTTCGA+GGG + chr1.3:59891117-59891136 MS.gene21012:intron 20.0%
!!! GATTTGTTAAATGAGTTTAA+AGG + chr1.3:59892616-59892635 MS.gene21012:intron 20.0%
!!! TATAACACATTTCAGTTTTA+AGG - chr1.3:59891243-59891262 None:intergenic 20.0%
!!! TTTTTCTTTTATTGCATGTT+TGG - chr1.3:59891075-59891094 None:intergenic 20.0%
! AAAATGCCTTGTAAAAACAT+TGG + chr1.3:59892077-59892096 MS.gene21012:intron 25.0%
! AAGACAAAGAAAAGAAGAAA+CGG + chr1.3:59892764-59892783 MS.gene21012:CDS 25.0%
! AGAACAAATTAAATCATGTG+AGG - chr1.3:59891547-59891566 None:intergenic 25.0%
! ATGCTTGATATGTAAATGTT+TGG + chr1.3:59892011-59892030 MS.gene21012:intron 25.0%
! ATTTCAAACAAACAAAGTAG+AGG - chr1.3:59893004-59893023 None:intergenic 25.0%
! CTCTTTAAATGTTTGGTATA+AGG + chr1.3:59891271-59891290 MS.gene21012:intron 25.0%
! CTTATACCAAACATTTAAAG+AGG - chr1.3:59891273-59891292 None:intergenic 25.0%
! GAAGATAAAGGAAAAAAAGA+AGG + chr1.3:59893073-59893092 MS.gene21012:CDS 25.0%
! TATAGACACAACATATTTGA+TGG - chr1.3:59891656-59891675 None:intergenic 25.0%
!! ATAAAGTAACAAACCTGTTT+TGG - chr1.3:59892547-59892566 None:intergenic 25.0%
!! ATGTCTCAATTTTTCTTTTG+TGG - chr1.3:59891202-59891221 None:intergenic 25.0%
!! ATTGGTCTAAGAAAATGTAT+TGG + chr1.3:59892095-59892114 MS.gene21012:intron 25.0%
!! GGATAAAAATATTTAGTTCG+AGG + chr1.3:59891116-59891135 MS.gene21012:intron 25.0%
!! TTTGGATTCTTATTTAAAGC+AGG + chr1.3:59892676-59892695 MS.gene21012:intron 25.0%
!!! TTCTTTTCTTTGTCATTTGA+GGG - chr1.3:59892739-59892758 None:intergenic 25.0%
AATCCTTATAACCTGTCATT+TGG - chr1.3:59892367-59892386 None:intergenic 30.0%
ACAGTAAGTATCCAAATCAA+CGG - chr1.3:59893318-59893337 None:intergenic 30.0%
AGAAAAGAAAGGAGACTTTA+AGG - chr1.3:59892221-59892240 None:intergenic 30.0%
AGACATGCATTTAGTCATAT+TGG + chr1.3:59891216-59891235 MS.gene21012:intron 30.0%
ATTGATCTCTTAGATACCTT+CGG - chr1.3:59891930-59891949 None:intergenic 30.0%
GATTACTAAAGTTCCAAAAC+AGG + chr1.3:59892531-59892550 MS.gene21012:intron 30.0%
GGATGTGAAATTAAATCACT+CGG + chr1.3:59892116-59892135 MS.gene21012:intron 30.0%
GTATCTAAGAGATCAATTTG+TGG + chr1.3:59891933-59891952 MS.gene21012:intron 30.0%
GTTCGTTGGATATGTTAAAT+GGG + chr1.3:59893336-59893355 MS.gene21012:CDS 30.0%
TATTTGATATCCACTTTGTC+CGG - chr1.3:59892803-59892822 None:intergenic 30.0%
TCATGAAGATAATCTGAATG+TGG + chr1.3:59892316-59892335 MS.gene21012:CDS 30.0%
TGTTCGTTGGATATGTTAAA+TGG + chr1.3:59893335-59893354 MS.gene21012:CDS 30.0%
TTATCATACCTCTTTATCAG+TGG - chr1.3:59892926-59892945 None:intergenic 30.0%
TTGAGAAATTGTACTTCTAC+AGG + chr1.3:59891885-59891904 MS.gene21012:CDS 30.0%
TTGGATTGAGGATAAATTTG+TGG - chr1.3:59891056-59891075 None:intergenic 30.0%
TTGTGGCAAAAACTACAAAT+TGG - chr1.3:59891039-59891058 None:intergenic 30.0%
TTGTTGACAGAAGAATAGTT+TGG - chr1.3:59893236-59893255 None:intergenic 30.0%
TTTATTGCATGTTTGGATTG+AGG - chr1.3:59891068-59891087 None:intergenic 30.0%
! ACATATTTGATGGATAGACT+TGG - chr1.3:59891646-59891665 None:intergenic 30.0%
! ATATCGATCTTGTTTCATTC+CGG + chr1.3:59891969-59891988 MS.gene21012:intron 30.0%
! ATTCACTGATTGAATGCAAA+AGG + chr1.3:59892413-59892432 MS.gene21012:intron 30.0%
! TCTCAATTTTTCTTTTGTGG+TGG - chr1.3:59891199-59891218 None:intergenic 30.0%
! TGTCAAACTAACTGAAGTTT+TGG + chr1.3:59890766-59890785 MS.gene21012:CDS 30.0%
! TTCAGTTAGTTTGACATAGA+AGG - chr1.3:59890762-59890781 None:intergenic 30.0%
!! CTTAGACCAATGTTTTTACA+AGG - chr1.3:59892086-59892105 None:intergenic 30.0%
!! CTTCTTTTCTTTGTCATTTG+AGG - chr1.3:59892740-59892759 None:intergenic 30.0%
!! TACTGGTTTATCTCGATTAA+TGG + chr1.3:59892292-59892311 MS.gene21012:CDS 30.0%
!!! CTTTTTTATGTAGCCATATG+TGG + chr1.3:59893040-59893059 MS.gene21012:intron 30.0%
AAGATCTTAAGGAGGAAAAG+AGG + chr1.3:59893165-59893184 MS.gene21012:CDS 35.0%
AATCATGTGAGGTCAATATG+TGG - chr1.3:59891536-59891555 None:intergenic 35.0%
AATGTTTGGTATAAGGTCAC+TGG + chr1.3:59891278-59891297 MS.gene21012:intron 35.0%
ACAGAAGAATAGTTTGGTAG+TGG - chr1.3:59893230-59893249 None:intergenic 35.0%
ACTAGCTAGTGACCTTTATT+GGG - chr1.3:59891148-59891167 None:intergenic 35.0%
ACTGCATATAAGTGAAGTAC+CGG - chr1.3:59891991-59892010 None:intergenic 35.0%
AGAATTGAAACAGAGTTGCA+TGG - chr1.3:59891412-59891431 None:intergenic 35.0%
AGATAATCTGAATGTGGCAA+AGG + chr1.3:59892322-59892341 MS.gene21012:CDS 35.0%
AGTAAGTATCCAAATCAACG+GGG - chr1.3:59893316-59893335 None:intergenic 35.0%
ATCCAAATATCCAAACATGC+AGG - chr1.3:59890969-59890988 None:intergenic 35.0%
CAGTAAGTATCCAAATCAAC+GGG - chr1.3:59893317-59893336 None:intergenic 35.0%
CTCTATCTCTAAGTCATCAT+TGG - chr1.3:59890738-59890757 None:intergenic 35.0%
CTTTACAAAATGCAAGTGTG+AGG - chr1.3:59892244-59892263 None:intergenic 35.0%
TAGCCAAATGACAGGTTATA+AGG + chr1.3:59892361-59892380 MS.gene21012:intron 35.0%
TCGATTAAAAGCTCATGATC+CGG + chr1.3:59892843-59892862 MS.gene21012:CDS 35.0%
TGAGGTCAATATGTGGATAA+CGG - chr1.3:59891529-59891548 None:intergenic 35.0%
TGTACTTATTAGCTGCAATG+TGG + chr1.3:59892566-59892585 MS.gene21012:intron 35.0%
TGTGAAATTAAATCACTCGG+TGG + chr1.3:59892119-59892138 MS.gene21012:intron 35.0%
TGTGTGCATTATGCTATGTT+TGG + chr1.3:59890889-59890908 MS.gene21012:intron 35.0%
TTCCTGCATGTTTGGATATT+TGG + chr1.3:59890964-59890983 MS.gene21012:intron 35.0%
TTTGGATACTTACTGTTCGT+TGG + chr1.3:59893322-59893341 MS.gene21012:CDS 35.0%
! ACAAGTCTAAGATTGTCTCT+CGG - chr1.3:59891453-59891472 None:intergenic 35.0%
! AGACTTGTACCTGTTTTACT+TGG + chr1.3:59891465-59891484 MS.gene21012:CDS 35.0%
! CTCACACTTGCATTTTGTAA+AGG + chr1.3:59892242-59892261 MS.gene21012:intron 35.0%
! GAAAAAAGTGTGTGTACTTG+TGG + chr1.3:59891095-59891114 MS.gene21012:intron 35.0%
! TTTGTAAAGGTACGCGTAAA+AGG + chr1.3:59892255-59892274 MS.gene21012:intron 35.0%
! TTTTATGTAGCCATATGTGG+AGG + chr1.3:59893043-59893062 MS.gene21012:intron 35.0%
!! AATGACTGAAAAAGCACTCA+AGG + chr1.3:59893280-59893299 MS.gene21012:CDS 35.0%
!! AGGTTGGTCTTCAAAACAAA+TGG + chr1.3:59891771-59891790 MS.gene21012:intron 35.0%
!! GGTTGGTCTTCAAAACAAAT+GGG + chr1.3:59891772-59891791 MS.gene21012:intron 35.0%
!!! ACTTTTTTGCCATCAATGTC+CGG - chr1.3:59892865-59892884 None:intergenic 35.0%
!!! ATTTGTTTTGAAGACCAACC+TGG - chr1.3:59891772-59891791 None:intergenic 35.0%
AAAGAAAAGAAGAAACGGCG+AGG + chr1.3:59892769-59892788 MS.gene21012:CDS 40.0%
AACAGTACCATTGGTGAGTT+TGG - chr1.3:59890711-59890730 None:intergenic 40.0%
AGTTGTCTCTGCACTGAAAT+TGG + chr1.3:59891799-59891818 MS.gene21012:CDS 40.0%
ATATCCAAACATGCAGGAAG+TGG - chr1.3:59890963-59890982 None:intergenic 40.0%
ATTGGACACAACAGTACCAT+TGG - chr1.3:59890720-59890739 None:intergenic 40.0%
CAAGTGTGAGGAGAAAAGAA+AGG - chr1.3:59892232-59892251 None:intergenic 40.0%
CTAGCTAGTGACCTTTATTG+GGG - chr1.3:59891147-59891166 None:intergenic 40.0%
GAGGTCAATATGTGGATAAC+GGG - chr1.3:59891528-59891547 None:intergenic 40.0%
GGAGGAAAGCAAGAAGATAA+AGG + chr1.3:59893061-59893080 MS.gene21012:CDS 40.0%
GGTGCTTATGCTGCAATATT+TGG + chr1.3:59892658-59892677 MS.gene21012:intron 40.0%
GGTGTTTCTTGTGACTTTAG+AGG + chr1.3:59892637-59892656 MS.gene21012:intron 40.0%
TCAGATACCAAACTCACCAA+TGG + chr1.3:59890701-59890720 MS.gene21012:CDS 40.0%
TGATTCTCTTGAACTTGTCC+AGG + chr1.3:59891751-59891770 MS.gene21012:intron 40.0%
TGGCAAAAAAGTTCTACGCA+TGG + chr1.3:59892873-59892892 MS.gene21012:CDS 40.0%
TGGTGACTTCCAAGTAAAAC+AGG - chr1.3:59891477-59891496 None:intergenic 40.0%
TTCTACAGGTTTCCTGTAAC+CGG + chr1.3:59891899-59891918 MS.gene21012:CDS 40.0%
TTCTTGCTTTCCTCCACATA+TGG - chr1.3:59893056-59893075 None:intergenic 40.0%
TTGGTGAGTTTGGTATCTGA+AGG - chr1.3:59890701-59890720 None:intergenic 40.0%
! CGTTCTCAGAGATTAGTCAT+CGG + chr1.3:59891168-59891187 MS.gene21012:intron 40.0%
! GACTAGCTAGTGACCTTTAT+TGG - chr1.3:59891149-59891168 None:intergenic 40.0%
! GTATCTGAAGGTGATGATGA+TGG - chr1.3:59890689-59890708 None:intergenic 40.0%
! GTGGTGACACAAGTTTTCAT+CGG + chr1.3:59892138-59892157 MS.gene21012:intron 40.0%
!! GAAGAGCACTCAAGATCTTA+AGG + chr1.3:59893154-59893173 MS.gene21012:CDS 40.0%
!! GAGCACTGAAGATCTTAAGA+AGG + chr1.3:59893124-59893143 MS.gene21012:CDS 40.0%
AGACATGAGATTGAACCCAG+AGG - chr1.3:59890795-59890814 None:intergenic 45.0%
ATCCAAATCAACGGGGAAGA+AGG - chr1.3:59893309-59893328 None:intergenic 45.0%
GAGCACTCAAGATCTTAAGG+AGG + chr1.3:59893157-59893176 MS.gene21012:CDS 45.0%
GATGTCTGCCACTGATAAAG+AGG + chr1.3:59892915-59892934 MS.gene21012:CDS 45.0%
GTCTCTGCACTGAAATTGGA+AGG + chr1.3:59891803-59891822 MS.gene21012:CDS 45.0%
TACTTGGAAGTCACCAGAAG+AGG + chr1.3:59891481-59891500 MS.gene21012:CDS 45.0%
TCCAAATCAACGGGGAAGAA+GGG - chr1.3:59893308-59893327 None:intergenic 45.0%
TCTCTTGAACTTGTCCAGGT+TGG + chr1.3:59891755-59891774 MS.gene21012:intron 45.0%
TGATATCCACTTTGTCCGGT+AGG - chr1.3:59892799-59892818 None:intergenic 45.0%
TTCTGTCAACAATGCAGCTG+TGG + chr1.3:59893244-59893263 MS.gene21012:CDS 45.0%
! ATTGCCACTTCCTGCATGTT+TGG + chr1.3:59890956-59890975 MS.gene21012:intron 45.0%
!! AATGTGGCAAAGGTGTCCAA+GGG + chr1.3:59892332-59892351 MS.gene21012:CDS 45.0%
!! TGGATAACGGGACAAATACC+TGG - chr1.3:59891516-59891535 None:intergenic 45.0%
!!! ACTGAAGTTTTGGACTCCTC+TGG + chr1.3:59890776-59890795 MS.gene21012:CDS 45.0%
!!! CTGAAGTTTTGGACTCCTCT+GGG + chr1.3:59890777-59890796 MS.gene21012:CDS 45.0%
AGCCAGCTGTCAAGAACCTT+GGG + chr1.3:59892699-59892718 MS.gene21012:CDS 50.0%
AGGAAGCAGAACTCGACTAC+TGG + chr1.3:59892275-59892294 MS.gene21012:CDS 50.0%
CAACAATGCAGCTGTGGAGT+TGG + chr1.3:59893250-59893269 MS.gene21012:CDS 50.0%
CATTTGGCTAGAGTAGCCCT+TGG - chr1.3:59892351-59892370 None:intergenic 50.0%
CTGGTGAAAACCTCCTCTTC+TGG - chr1.3:59891497-59891516 None:intergenic 50.0%
GAAAAGAGGCCTAGTGTGTG+CGG + chr1.3:59893179-59893198 MS.gene21012:CDS 50.0%
GCTCATGATCCGGACATTGA+TGG + chr1.3:59892853-59892872 MS.gene21012:CDS 50.0%
GGCTACTCTAGCCAAATGAC+AGG + chr1.3:59892353-59892372 MS.gene21012:CDS 50.0%
TACGCATGGCTGTTGATGCT+TGG + chr1.3:59892887-59892906 MS.gene21012:CDS 50.0%
TAGTCGTAACCGCACACACT+AGG - chr1.3:59893191-59893210 None:intergenic 50.0%
TATCCACTTTGTCCGGTAGG+AGG - chr1.3:59892796-59892815 None:intergenic 50.0%
TTGGAAGTCACCAGAAGAGG+AGG + chr1.3:59891484-59891503 MS.gene21012:CDS 50.0%
! CAGAAGAGGAGGTTTTCACC+AGG + chr1.3:59891495-59891514 MS.gene21012:CDS 50.0%
! TCCCTTCTTCCCCGTTGATT+TGG + chr1.3:59893304-59893323 MS.gene21012:CDS 50.0%
!! GAATGTGGCAAAGGTGTCCA+AGG + chr1.3:59892331-59892350 MS.gene21012:CDS 50.0%
!! TTAGATACCTTCGGTGCTGC+CGG - chr1.3:59891921-59891940 None:intergenic 50.0%
CACCCAAGGTTCTTGACAGC+TGG - chr1.3:59892704-59892723 None:intergenic 55.0%
GAGCCAGCTGTCAAGAACCT+TGG + chr1.3:59892698-59892717 MS.gene21012:CDS 55.0%
TCGAGGGGAGACCCCAATAA+AGG + chr1.3:59891133-59891152 MS.gene21012:intron 55.0%
!! GGTGCTTGTAGAAGCACCCA+AGG - chr1.3:59892718-59892737 None:intergenic 55.0%
AAACGGCGAGGTCCTCCTAC+CGG + chr1.3:59892781-59892800 MS.gene21012:CDS 60.0%
GGTCCTCCTACCGGACAAAG+TGG + chr1.3:59892790-59892809 MS.gene21012:CDS 60.0%
! CCTGTAACCGGCAGCACCGA+AGG + chr1.3:59891911-59891930 MS.gene21012:CDS 65.0%
!! CCTTCGGTGCTGCCGGTTAC+AGG - chr1.3:59891914-59891933 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.3 gene 59890662 59893370 59890662 ID=MS.gene21012
chr1.3 mRNA 59890662 59893370 59890662 ID=MS.gene21012.t1;Parent=MS.gene21012
chr1.3 exon 59890662 59890810 59890662 ID=MS.gene21012.t1.exon1;Parent=MS.gene21012.t1
chr1.3 CDS 59890662 59890810 59890662 ID=cds.MS.gene21012.t1;Parent=MS.gene21012.t1
chr1.3 exon 59891441 59891516 59891441 ID=MS.gene21012.t1.exon2;Parent=MS.gene21012.t1
chr1.3 CDS 59891441 59891516 59891441 ID=cds.MS.gene21012.t1;Parent=MS.gene21012.t1
chr1.3 exon 59891773 59891932 59891773 ID=MS.gene21012.t1.exon3;Parent=MS.gene21012.t1
chr1.3 CDS 59891773 59891932 59891773 ID=cds.MS.gene21012.t1;Parent=MS.gene21012.t1
chr1.3 exon 59892264 59892374 59892264 ID=MS.gene21012.t1.exon4;Parent=MS.gene21012.t1
chr1.3 CDS 59892264 59892374 59892264 ID=cds.MS.gene21012.t1;Parent=MS.gene21012.t1
chr1.3 exon 59892698 59892936 59892698 ID=MS.gene21012.t1.exon5;Parent=MS.gene21012.t1
chr1.3 CDS 59892698 59892936 59892698 ID=cds.MS.gene21012.t1;Parent=MS.gene21012.t1
chr1.3 exon 59893053 59893370 59893053 ID=MS.gene21012.t1.exon6;Parent=MS.gene21012.t1
chr1.3 CDS 59893053 59893370 59893053 ID=cds.MS.gene21012.t1;Parent=MS.gene21012.t1
Gene Sequence

>MS.gene21012

ATGGAAGGCAACAAGACTATGGTTCCATCATCATCACCTTCAGATACCAAACTCACCAATGGTACTGTTGTGTCCAATGATGACTTAGAGATAGAGACCTTCTATGTCAAACTAACTGAAGTTTTGGACTCCTCTGGGTTCAATCTCATGTCTGTTTCTTCAATCAGCTTCTTACTTTCTGTTTTGCTTTTATTTTTTTCGTTTGTATTTGCATGAGTGTTTTTACTTGTGTGCATTATGCTATGTTTGGTTTTACAAAATTCGCATGCAAAAAACGTCAAGCATTTTTTTTTAATTGCCACTTCCTGCATGTTTGGATATTTGGATTCGCTGTAAATTTGTAAAAATAACAGTGACATACTTGATTTCATTCTCCAATTTGTAGTTTTTGCCACAAATTTATCCTCAATCCAAACATGCAATAAAAGAAAAAGAAAAAAGTGTGTGTACTTGTGGATAAAAATATTTAGTTCGAGGGGAGACCCCAATAAAGGTCACTAGCTAGTCGTTCTCAGAGATTAGTCATCGGCAAAGCCACCACAAAAGAAAAATTGAGACATGCATTTAGTCATATTGGTCCTTAAAACTGAAATGTGTTATAATTTATTCCTCTTTAAATGTTTGGTATAAGGTCACTGGAATAGTAGAATGTGTTACAATTTAGAAAGAAGAATACTTGTAATATTATATAAAGTTTTATGAGATTATTTGGATAAAAAAAAATGATGGATAATAAAGGGTATATGACCATGCAACTCTGTTTCAATTCTACATGACAGTTTCAATGTCCGAGAGACAATCTTAGACTTGTACCTGTTTTACTTGGAAGTCACCAGAAGAGGAGGTTTTCACCAGGTATTTGTCCCGTTATCCACATATTGACCTCACATGATTTAATTTGTTCTATTTGTTATATGTTCATTTCTCATTAGTGCATGTGTATATATTTTATGCATGTAGCTTCTATTATTTGCATGCACACCAAGTCTATCCATCAAATATGTTGTGTCTATATAATACAAATTAGTCAATAGAGGCTTTTAATTAAGTAATTATCGTATGAATTGTTCAAATTTAATATATTGTAATTGATTCTCTTGAACTTGTCCAGGTTGGTCTTCAAAACAAATGGGCTGAAGTTGTCTCTGCACTGAAATTGGAAGGCAGCAATGCAATGTTATCTGCTCAAGTTGAAAAGATCTATGCAACACTTCTATACAAATTTGAGAAATTGTACTTCTACAGGTTTCCTGTAACCGGCAGCACCGAAGGTATCTAAGAGATCAATTTGTGGTTATTTTACGTAGATATCGATCTTGTTTCATTCCGGTACTTCACTTATATGCAGTATGCTTGATATGTAAATGTTTGGTAGATTATACATAATATGATTTTAATTTATGACTAGTAATAGAAAAATGCCTTGTAAAAACATTGGTCTAAGAAAATGTATTGGATGTGAAATTAAATCACTCGGTGGTGACACAAGTTTTCATCGGATCATTGTATGATGTTTTTATTACGTTTTTGTTTTAGGATGAAAATGTGTTCTTTAACCTTAAAGTCTCCTTTCTTTTCTCCTCACACTTGCATTTTGTAAAGGTACGCGTAAAAGGAAGCAGAACTCGACTACTGGTTTATCTCGATTAATGGCTCATGAAGATAATCTGAATGTGGCAAAGGTGTCCAAGGGCTACTCTAGCCAAATGACAGGTTATAAGGATTTAGAATATTTATGCATTGAAAATTTGATTCACTGATTGAATGCAAAAGGTTGCACTCAAGTACTAATGAATTAACTAGCTAACTAGCTGAATCAAAACTAATATCACAACAGAACACAATTTATCAACTAATCATGATAACTGAGATTACTAAAGTTCCAAAACAGGTTTGTTACTTTATGTACTTATTAGCTGCAATGTGGACATTTGTTTTCTTTAGTAACTCAAGTGATTTGTTAAATGAGTTTAAAGGTGTTTCTTGTGACTTTAGAGGTGCTTATGCTGCAATATTTGGATTCTTATTTAAAGCAGGAGCCAGCTGTCAAGAACCTTGGGTGCTTCTACAAGCACCCTCAAATGACAAAGAAAAGAAGAAACAAGACAAAGAAAAGAAGAAACGGCGAGGTCCTCCTACCGGACAAAGTGGATATCAAATATTTCTCAAGCATGAATGTGCTCGATTAAAAGCTCATGATCCGGACATTGATGGCAAAAAAGTTCTACGCATGGCTGTTGATGCTTGGCAGAAGATGTCTGCCACTGATAAAGAGGTATGATAAATAAAATTGACATAATATCGATCAAATGTCGAACAATTTATATTTTTGCAATTAGCCTCTACTTTGTTTGTTTGAAATTTTCCATTTATAATTACTTTTTTATGTAGCCATATGTGGAGGAAAGCAAGAAGATAAAGGAAAAAAAGAAGGAAGCAGTGATCATTGACAATAAACAGATGAGCACTGAAGATCTTAAGAAGGATGAAAAGAAGAGCACTCAAGATCTTAAGGAGGAAAAGAGGCCTAGTGTGTGCGGTTACGACTACTGTGCAACTTCGCAACCACTACCAAACTATTCTTCTGTCAACAATGCAGCTGTGGAGTTGGCTTTGAAAATGACTGAAAAAGCACTCAAGGATCCCTTCTTCCCCGTTGATTTGGATACTTACTGTTCGTTGGATATGTTAAATGGGAAATCCAAATGA
Protein sequence

>MS.gene21012.t1

MEGNKTMVPSSSPSDTKLTNGTVVSNDDLEIETFYVKLTEVLDSSGFNLIFNVRETILDLYLFYLEVTRRGGFHQVGLQNKWAEVVSALKLEGSNAMLSAQVEKIYATLLYKFEKLYFYRFPVTGSTEGTRKRKQNSTTGLSRLMAHEDNLNVAKVSKGYSSQMTGASCQEPWVLLQAPSNDKEKKKQDKEKKKRRGPPTGQSGYQIFLKHECARLKAHDPDIDGKKVLRMAVDAWQKMSATDKEPYVEESKKIKEKKKEAVIIDNKQMSTEDLKKDEKKSTQDLKEEKRPSVCGYDYCATSQPLPNYSSVNNAAVELALKMTEKALKDPFFPVDLDTYCSLDMLNGKSK