AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21032

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21032.t1 MTR_5g095070 61.056 303 82 8 2 304 5 271 1.25e-111 326
MS.gene21032.t1 MTR_1g084150 76.824 233 28 2 150 356 1 233 1.25e-110 322
MS.gene21032.t1 MTR_5g080030 64.935 231 73 2 37 267 44 266 4.83e-98 296
MS.gene21032.t1 MTR_5g080030 53.254 169 58 6 189 356 264 412 6.76e-38 140
MS.gene21032.t1 MTR_4g015240 51.220 82 19 2 265 345 39 100 1.07e-16 75.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 78 sgRNAs with CRISPR-Local

Find 160 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTTCGTTGGATATGTTAAA+TGG 0.182684 1.4:-68882396 MS.gene21032:CDS
CACAATTCCATTGGTGAGTT+TGG 0.209381 1.4:+68884996 None:intergenic
TACTGGTTTATCTCAATTAA+TGG 0.258551 1.4:-68883437 MS.gene21032:CDS
TCCCTTCTTCCCAGTTGATT+TGG 0.271998 1.4:-68882427 MS.gene21032:CDS
CCTTGGGCGCTGCCGGTTGC+AGG 0.289338 1.4:+68883810 None:intergenic
TGTCAAACTAACTGAAGTTT+TGG 0.307954 1.4:-68884920 MS.gene21032:CDS
AGTTGTCTCTGCACTCAAAT+TGG 0.316435 1.4:-68883922 MS.gene21032:CDS
TTCTTGCTTTCCTCCACATA+TGG 0.365771 1.4:+68882678 None:intergenic
TTTGTAAAGGTACGCGTAAA+AGG 0.367652 1.4:-68883474 MS.gene21032:intron
TTCAGTTAGTTTGACATAGA+AGG 0.372838 1.4:+68884927 None:intergenic
CAGTAAGTATCCAAATCAAC+TGG 0.378693 1.4:+68882417 None:intergenic
TTCTACAGGTTTCCTGCAAC+CGG 0.391216 1.4:-68883822 MS.gene21032:CDS
GAAGAGCACTCAAGATCTTA+AGG 0.392322 1.4:-68882577 MS.gene21032:CDS
AGGTTGGTCTTCAAAACAAA+TGG 0.395740 1.4:-68883950 MS.gene21032:CDS
TTGAGAAATTGTACTTCTAC+AGG 0.399495 1.4:-68883836 MS.gene21032:CDS
AATCCTTATAACCTGTCATT+TGG 0.408344 1.4:+68883365 None:intergenic
CAACCGCTAGTATAGTCATT+TGG 0.413628 1.4:+68882368 None:intergenic
GGAGGAAAGCAAGAAGATAA+AGG 0.418994 1.4:-68882670 MS.gene21032:CDS
AATGGAAGGCAACAAAACTA+TGG 0.422265 1.4:-68885040 MS.gene21032:CDS
GTCTCTATCTCTAAGTCATC+AGG 0.427299 1.4:+68884949 None:intergenic
TGGTGACTTCCAAGTAAAAC+AGG 0.431064 1.4:+68884237 None:intergenic
TTAGATACCTTGGGCGCTGC+CGG 0.439165 1.4:+68883803 None:intergenic
CTGGTGAAAACCTCCTCTTC+TGG 0.440306 1.4:+68884217 None:intergenic
CCCATTGGACACAATTCCAT+TGG 0.444629 1.4:+68884987 None:intergenic
ATCCAAATCAACTGGGAAGA+AGG 0.454997 1.4:+68882425 None:intergenic
GCTCAAGATCCGGACATTGA+TGG 0.455328 1.4:-68882878 MS.gene21032:CDS
GTTCGTTGGATATGTTAAAT+GGG 0.459203 1.4:-68882395 MS.gene21032:CDS
TATTTGATATCCACTTTGTC+CGG 0.459963 1.4:+68882931 None:intergenic
AAGACAAAGAAAAGAAGAAA+CGG 0.466286 1.4:-68882967 MS.gene21032:CDS
TACGCATGGCTGTTGATGCT+TGG 0.488165 1.4:-68882844 MS.gene21032:CDS
CACCCGAGGTTCTTGACAGC+CGG 0.488188 1.4:+68883030 None:intergenic
TTTGGATACTTACTGTTCGT+TGG 0.489477 1.4:-68882409 MS.gene21032:CDS
AGCCGGCTGTCAAGAACCTC+GGG 0.489585 1.4:-68883032 MS.gene21032:CDS
ACAGAAGAATAGTTTGGTAG+TGG 0.492096 1.4:+68882504 None:intergenic
CCAATGGAATTGTGTCCAAT+GGG 0.492590 1.4:-68884987 MS.gene21032:CDS
TTCTTAATTAAAGCAGGAGC+CGG 0.497515 1.4:-68883049 MS.gene21032:intron
AGGAAGCAGAACTCGACTAC+TGG 0.510036 1.4:-68883454 MS.gene21032:CDS
CCTGCAACCGGCAGCGCCCA+AGG 0.510627 1.4:-68883810 MS.gene21032:intron
ACCAATGGAATTGTGTCCAA+TGG 0.516036 1.4:-68884988 MS.gene21032:CDS
ATCGAGCACATTCATGCTTG+AGG 0.516753 1.4:+68882906 None:intergenic
CAACAATGCAGCTGTGGAGT+TGG 0.523389 1.4:-68882481 MS.gene21032:CDS
TTGTTGACAGAAGAATAGTT+TGG 0.526138 1.4:+68882498 None:intergenic
TCCAAATCAACTGGGAAGAA+GGG 0.526508 1.4:+68882426 None:intergenic
TTCTGTCAACAATGCAGCTG+TGG 0.527890 1.4:-68882487 MS.gene21032:CDS
CATTTGGCTAGAGTAGTCCT+TGG 0.531068 1.4:+68883381 None:intergenic
TTGGCATCTGAAGGTGATGA+TGG 0.536024 1.4:+68885015 None:intergenic
TACTTGGAAGTCACCAGAAG+AGG 0.540823 1.4:-68884230 MS.gene21032:CDS
GATCATTGACAATAAACAGA+TGG 0.543332 1.4:-68882628 MS.gene21032:CDS
GGTTGGTCTTCAAAACAAAT+GGG 0.543380 1.4:-68883949 MS.gene21032:CDS
TCGATTAAAAGCTCAAGATC+CGG 0.550386 1.4:-68882888 MS.gene21032:CDS
TGATATCCACTTTGTCCGGT+AGG 0.555314 1.4:+68882935 None:intergenic
GAGCCGGCTGTCAAGAACCT+CGG 0.562075 1.4:-68883033 MS.gene21032:CDS
GACTACTCTAGCCAAATGAC+AGG 0.564216 1.4:-68883376 MS.gene21032:intron
AGAAGAGTACTGTTTCCCAT+TGG 0.564784 1.4:+68884972 None:intergenic
GTCTCTGCACTCAAATTGGA+AGG 0.566571 1.4:-68883918 MS.gene21032:CDS
AAGATCTTAAGGAGGAAAAG+AGG 0.571062 1.4:-68882566 MS.gene21032:CDS
AAACGGCGAGGTCCTCCTAC+CGG 0.572639 1.4:-68882950 MS.gene21032:CDS
TTCCTAGCTCAAACAATGGA+AGG 0.574513 1.4:-68885054 None:intergenic
TAGTCGTAACCGCACACACT+AGG 0.575038 1.4:+68882543 None:intergenic
GAATGTGGCAAAGGTTTCCA+AGG 0.580859 1.4:-68883398 MS.gene21032:CDS
GGGCACTGAAGATCTTAAGA+AGG 0.581617 1.4:-68882607 MS.gene21032:CDS
ATCATTGACAATAAACAGAT+GGG 0.586725 1.4:-68882627 MS.gene21032:CDS
AGATAATCTGAATGTGGCAA+AGG 0.590122 1.4:-68883407 MS.gene21032:CDS
TTGGAAGTCACCAGAAGAGG+AGG 0.590853 1.4:-68884227 MS.gene21032:CDS
TATCCACTTTGTCCGGTAGG+AGG 0.594215 1.4:+68882938 None:intergenic
TTGGTGAGTTTGGCATCTGA+AGG 0.614422 1.4:+68885006 None:intergenic
TCAGATGCCAAACTCACCAA+TGG 0.618895 1.4:-68885003 MS.gene21032:CDS
TTTCATGTAGCCATATGTGG+AGG 0.619171 1.4:-68882688 MS.gene21032:intron
GGTCCTCCTACCGGACAAAG+TGG 0.623840 1.4:-68882941 MS.gene21032:CDS
ACAAGTCTAAGATTGTCTCT+CGG 0.631739 1.4:+68884261 None:intergenic
AAGCATCAACAGCCATGCGT+AGG 0.637247 1.4:+68882846 None:intergenic
GAAAAGAGGCCTAGTGTGTG+CGG 0.639000 1.4:-68882552 MS.gene21032:CDS
AGTAAGTATCCAAATCAACT+GGG 0.663991 1.4:+68882418 None:intergenic
GAGCACTCAAGATCTTAAGG+AGG 0.665195 1.4:-68882574 MS.gene21032:CDS
GGTGCTTGTAGAAGCACCCG+AGG 0.679117 1.4:+68883016 None:intergenic
TGATGAAGATAATCTGAATG+TGG 0.680646 1.4:-68883413 MS.gene21032:CDS
AAAGAAAAGAAGAAACGGCG+AGG 0.681943 1.4:-68882962 MS.gene21032:CDS
AGACATGAGATTGAACCCAG+AGG 0.774697 1.4:+68884894 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CATGAAAAAGTAATTATAAA+TGG + chr1.4:68884721-68884740 None:intergenic 15.0%
!! TCAGATTTAATATATTGTAA+TGG - chr1.4:68883431-68883450 MS.gene21032:CDS 15.0%
!!! AAAGTTTTATGAGATAATAA+AGG - chr1.4:68883089-68883108 MS.gene21032:intron 15.0%
!!! AAGTTTTATGAGATAATAAA+GGG - chr1.4:68883090-68883109 MS.gene21032:intron 15.0%
!! AAATTAAATCATGTGATCAT+GGG + chr1.4:68883261-68883280 None:intergenic 20.0%
!! ATGCTTGATATATAGATATT+TGG - chr1.4:68883701-68883720 MS.gene21032:intron 20.0%
!! ATTATATAGACACAACATAT+TGG + chr1.4:68883360-68883379 None:intergenic 20.0%
!! TAATATAAATTAGTCAAGAG+AGG - chr1.4:68883376-68883395 MS.gene21032:intron 20.0%
!! TGAAATGAAATTAAATCACT+CGG - chr1.4:68883812-68883831 MS.gene21032:CDS 20.0%
!! TTTATACCAAACATTTAAAG+AGG + chr1.4:68882976-68882995 None:intergenic 20.0%
!!! GATTTGTTAAATGAGTTTAA+AGG - chr1.4:68884307-68884326 MS.gene21032:intron 20.0%
! AAACATGCACTAAAAGAAAA+AGG - chr1.4:68882803-68882822 MS.gene21032:intron 25.0%
! AACTCATTTAACAAATCACT+TGG + chr1.4:68884304-68884323 None:intergenic 25.0%
! AAGACAAAGAAAAGAAGAAA+CGG - chr1.4:68884455-68884474 MS.gene21032:intron 25.0%
! ATAAAAAATGTAGTTGGAGA+AGG - chr1.4:68882847-68882866 MS.gene21032:CDS 25.0%
! ATCATTGACAATAAACAGAT+GGG - chr1.4:68884795-68884814 MS.gene21032:intron 25.0%
! ATTTCAAACAAACAAAGTAG+AGG + chr1.4:68884695-68884714 None:intergenic 25.0%
! CAAATTAAATCATGTGATCA+TGG + chr1.4:68883262-68883281 None:intergenic 25.0%
! GAAGATAAAGGAAAAAAAGA+AGG - chr1.4:68884764-68884783 MS.gene21032:intron 25.0%
! TGTTTGGATATTTGGATTTA+CGG - chr1.4:68882708-68882727 MS.gene21032:intron 25.0%
! TTGTGGATAAAAAATGTAGT+TGG - chr1.4:68882841-68882860 MS.gene21032:CDS 25.0%
! TTTGGATTCTTAATTAAAGC+AGG - chr1.4:68884367-68884386 MS.gene21032:intron 25.0%
! TTTGTTCCTCTTTAAATGTT+TGG - chr1.4:68882967-68882986 MS.gene21032:CDS 25.0%
!! AAAAGCCTTGTAAAAAACAT+TGG - chr1.4:68883768-68883787 MS.gene21032:intron 25.0%
!! ATAAAGTAACAAACCTGTTT+TGG + chr1.4:68884237-68884256 None:intergenic 25.0%
!! TACAACACATTTCAGTTTTA+AGG + chr1.4:68882946-68882965 None:intergenic 25.0%
!! TTTTTCTTTTAGTGCATGTT+TGG + chr1.4:68882804-68882823 None:intergenic 25.0%
!!! TACTGGTTTATCTCAATTAA+TGG - chr1.4:68883985-68884004 MS.gene21032:intron 25.0%
!!! TTGTTTTCTTTGTCATTTGA+GGG + chr1.4:68884430-68884449 None:intergenic 25.0%
AATCCTTATAACCTGTCATT+TGG + chr1.4:68884060-68884079 None:intergenic 30.0%
AATGAAATTAAATCACTCGG+TGG - chr1.4:68883815-68883834 MS.gene21032:CDS 30.0%
AATTGATCTCTTAGATACCT+TGG + chr1.4:68883632-68883651 None:intergenic 30.0%
AGAAAAGAAAGGAGACTTTA+AGG + chr1.4:68883914-68883933 None:intergenic 30.0%
AGAATGTGCTTCAATTTAGA+AGG - chr1.4:68883014-68883033 MS.gene21032:CDS 30.0%
AGACATGCATTTAGTCATAT+TGG - chr1.4:68882919-68882938 MS.gene21032:CDS 30.0%
AGTAAGTATCCAAATCAACT+GGG + chr1.4:68885007-68885026 None:intergenic 30.0%
ATTGATCTCTTAGATACCTT+GGG + chr1.4:68883631-68883650 None:intergenic 30.0%
GAATGTGCTTCAATTTAGAA+GGG - chr1.4:68883015-68883034 MS.gene21032:CDS 30.0%
GATCATTGACAATAAACAGA+TGG - chr1.4:68884794-68884813 MS.gene21032:intron 30.0%
GATTACTAAAGTTCCAAAAC+AGG - chr1.4:68884221-68884240 MS.gene21032:CDS 30.0%
GTATCTAAGAGATCAATTTG+TGG - chr1.4:68883634-68883653 MS.gene21032:intron 30.0%
GTTCGTTGGATATGTTAAAT+GGG - chr1.4:68885027-68885046 MS.gene21032:CDS 30.0%
TATTTGATATCCACTTTGTC+CGG + chr1.4:68884494-68884513 None:intergenic 30.0%
TGATGAAGATAATCTGAATG+TGG - chr1.4:68884009-68884028 MS.gene21032:intron 30.0%
TGTTCGTTGGATATGTTAAA+TGG - chr1.4:68885026-68885045 MS.gene21032:CDS 30.0%
TTGAGAAATTGTACTTCTAC+AGG - chr1.4:68883586-68883605 MS.gene21032:intron 30.0%
TTGATCCACTGATATGTAAA+AGG - chr1.4:68884103-68884122 MS.gene21032:intron 30.0%
TTGTGGCAAAAACTACAAAT+TGG + chr1.4:68882775-68882794 None:intergenic 30.0%
TTGTTGACAGAAGAATAGTT+TGG + chr1.4:68884927-68884946 None:intergenic 30.0%
! ATGTCTCGATTTTTCTTTTG+TGG + chr1.4:68882905-68882924 None:intergenic 30.0%
! ATTGGTCTAGGAAAATGTAT+TGG - chr1.4:68883786-68883805 MS.gene21032:intron 30.0%
! CTTGTAAAAAACATTGGTCT+AGG - chr1.4:68883774-68883793 MS.gene21032:intron 30.0%
! GTAGATATCTTGTTTCATTC+TGG - chr1.4:68883666-68883685 MS.gene21032:intron 30.0%
! TGTCAAACTAACTGAAGTTT+TGG - chr1.4:68882502-68882521 MS.gene21032:CDS 30.0%
! TTCAGTTAGTTTGACATAGA+AGG + chr1.4:68882498-68882517 None:intergenic 30.0%
!! CTTGTTTTCTTTGTCATTTG+AGG + chr1.4:68884431-68884450 None:intergenic 30.0%
!! TTATCATACCTGTTTTTCAG+TGG + chr1.4:68884617-68884636 None:intergenic 30.0%
!!! CTAGACCAATGTTTTTTACA+AGG + chr1.4:68883776-68883795 None:intergenic 30.0%
AAGATCTTAAGGAGGAAAAG+AGG - chr1.4:68884856-68884875 MS.gene21032:intron 35.0%
ACAGAAGAATAGTTTGGTAG+TGG + chr1.4:68884921-68884940 None:intergenic 35.0%
ACATATTGGATCGATAGACT+TGG + chr1.4:68883346-68883365 None:intergenic 35.0%
AGATAATCTGAATGTGGCAA+AGG - chr1.4:68884015-68884034 MS.gene21032:intron 35.0%
AGGTCAATATGTGGATAACA+GGG + chr1.4:68883238-68883257 None:intergenic 35.0%
ATCCAAATATCCAAACATGC+AGG + chr1.4:68882705-68882724 None:intergenic 35.0%
CAGTAAGTATCCAAATCAAC+TGG + chr1.4:68885008-68885027 None:intergenic 35.0%
CTTTACAAAATGTAGGTGTG+AGG + chr1.4:68883937-68883956 None:intergenic 35.0%
GGTGTATCTTGTGAATTTAG+AGG - chr1.4:68884328-68884347 MS.gene21032:intron 35.0%
GTCAAGTAGAATTGAAACAG+AGG + chr1.4:68883130-68883149 None:intergenic 35.0%
TAGCCAAATGACAGGTTATA+AGG - chr1.4:68884054-68884073 MS.gene21032:intron 35.0%
TCACTTCTCATTAATGCATG+TGG - chr1.4:68883299-68883318 MS.gene21032:intron 35.0%
TCGATTAAAAGCTCAAGATC+CGG - chr1.4:68884534-68884553 MS.gene21032:intron 35.0%
TGCATGTTTGGATGAATTTG+TGG + chr1.4:68882792-68882811 None:intergenic 35.0%
TGCTTCAATTTAGAAGGGAA+AGG - chr1.4:68883020-68883039 MS.gene21032:CDS 35.0%
TGTACTTATTAGCTGCAATG+TGG - chr1.4:68884256-68884275 MS.gene21032:CDS 35.0%
TGTGTGCATTATGCTATGTT+TGG - chr1.4:68882625-68882644 MS.gene21032:CDS 35.0%
TTAAATCATGTGATCATGGG+AGG + chr1.4:68883258-68883277 None:intergenic 35.0%
TTCCTGCATGTTTGGATATT+TGG - chr1.4:68882700-68882719 MS.gene21032:intron 35.0%
TTCTTAATTAAAGCAGGAGC+CGG - chr1.4:68884373-68884392 MS.gene21032:intron 35.0%
TTTGGATACTTACTGTTCGT+TGG - chr1.4:68885013-68885032 MS.gene21032:CDS 35.0%
! ACAAGTCTAAGATTGTCTCT+CGG + chr1.4:68883164-68883183 None:intergenic 35.0%
! AGACTTGTACCTGTTTTACT+TGG - chr1.4:68883176-68883195 MS.gene21032:intron 35.0%
! CTCACACCTACATTTTGTAA+AGG - chr1.4:68883935-68883954 MS.gene21032:CDS 35.0%
! GCATAGATCTTTTCAACTTG+AGG + chr1.4:68883549-68883568 None:intergenic 35.0%
! TGCAACCTTTTACATATCAG+TGG + chr1.4:68884111-68884130 None:intergenic 35.0%
! TTTGTAAAGGTACGCGTAAA+AGG - chr1.4:68883948-68883967 MS.gene21032:CDS 35.0%
!! AATGACTGAAAAAGCACTCA+AGG - chr1.4:68884971-68884990 MS.gene21032:CDS 35.0%
!! AGGTTGGTCTTCAAAACAAA+TGG - chr1.4:68883472-68883491 MS.gene21032:intron 35.0%
!! CTTTTTCATGTAGCCATATG+TGG - chr1.4:68884731-68884750 MS.gene21032:intron 35.0%
!! GGTTGGTCTTCAAAACAAAT+GGG - chr1.4:68883473-68883492 MS.gene21032:intron 35.0%
!!! ACTTTTTTGCCATCAATGTC+CGG + chr1.4:68884556-68884575 None:intergenic 35.0%
!!! ATTTGTTTTGAAGACCAACC+TGG + chr1.4:68883473-68883492 None:intergenic 35.0%
AAAGAAAAGAAGAAACGGCG+AGG - chr1.4:68884460-68884479 MS.gene21032:intron 40.0%
ACCAATGGAATTGTGTCCAA+TGG - chr1.4:68882434-68882453 MS.gene21032:CDS 40.0%
AGTTGTCTCTGCACTCAAAT+TGG - chr1.4:68883500-68883519 MS.gene21032:intron 40.0%
ATATCCAAACATGCAGGAAG+TGG + chr1.4:68882699-68882718 None:intergenic 40.0%
ATCCAAATCAACTGGGAAGA+AGG + chr1.4:68885000-68885019 None:intergenic 40.0%
CACAATTCCATTGGTGAGTT+TGG + chr1.4:68882429-68882448 None:intergenic 40.0%
CACTAGCTAGTCATAGTCAT+CGG - chr1.4:68882871-68882890 MS.gene21032:CDS 40.0%
CCAATGGAATTGTGTCCAAT+GGG - chr1.4:68882435-68882454 MS.gene21032:CDS 40.0%
CGCGTACCTTTACAAAATGT+AGG + chr1.4:68883944-68883963 None:intergenic 40.0%
GAGGTCAATATGTGGATAAC+AGG + chr1.4:68883239-68883258 None:intergenic 40.0%
GGAGGAAAGCAAGAAGATAA+AGG - chr1.4:68884752-68884771 MS.gene21032:intron 40.0%
GGATTCTCTTGAATTTGTCC+AGG - chr1.4:68883452-68883471 MS.gene21032:CDS 40.0%
GGTGCTTATGCTGCAATATT+TGG - chr1.4:68884349-68884368 MS.gene21032:intron 40.0%
GTCTCTATCTCTAAGTCATC+AGG + chr1.4:68882476-68882495 None:intergenic 40.0%
GTGGTGACACAAGTTTACAT+CGG - chr1.4:68883834-68883853 MS.gene21032:CDS 40.0%
TAGGTGTGAGGAGAAAAGAA+AGG + chr1.4:68883925-68883944 None:intergenic 40.0%
TCCAAATCAACTGGGAAGAA+GGG + chr1.4:68884999-68885018 None:intergenic 40.0%
TCTCTTGAATTTGTCCAGGT+TGG - chr1.4:68883456-68883475 MS.gene21032:CDS 40.0%
TGGTGACTTCCAAGTAAAAC+AGG + chr1.4:68883188-68883207 None:intergenic 40.0%
TTCTTGCTTTCCTCCACATA+TGG + chr1.4:68884747-68884766 None:intergenic 40.0%
TTTCATGTAGCCATATGTGG+AGG - chr1.4:68884734-68884753 MS.gene21032:intron 40.0%
! AGAATTGAAACAGAGGTGCA+TGG + chr1.4:68883123-68883142 None:intergenic 40.0%
! GGAAAAAGTGTGTGTACTTG+TGG - chr1.4:68882824-68882843 MS.gene21032:CDS 40.0%
!! AGAAGAGTACTGTTTCCCAT+TGG + chr1.4:68882453-68882472 None:intergenic 40.0%
!! GAAGAGCACTCAAGATCTTA+AGG - chr1.4:68884845-68884864 MS.gene21032:intron 40.0%
AGACATGAGATTGAACCCAG+AGG + chr1.4:68882531-68882550 None:intergenic 45.0%
ATCGAGCACATTCATGCTTG+AGG + chr1.4:68884519-68884538 None:intergenic 45.0%
CATTTGGCTAGAGTAGTCCT+TGG + chr1.4:68884044-68884063 None:intergenic 45.0%
CCCATTGGACACAATTCCAT+TGG + chr1.4:68882438-68882457 None:intergenic 45.0%
GAATGTGGCAAAGGTTTCCA+AGG - chr1.4:68884024-68884043 MS.gene21032:intron 45.0%
GACTACTCTAGCCAAATGAC+AGG - chr1.4:68884046-68884065 MS.gene21032:intron 45.0%
GAGCACTCAAGATCTTAAGG+AGG - chr1.4:68884848-68884867 MS.gene21032:intron 45.0%
GATCATGGGAGGTCAATATG+TGG + chr1.4:68883247-68883266 None:intergenic 45.0%
GATGTCTGCCACTGAAAAAC+AGG - chr1.4:68884606-68884625 MS.gene21032:intron 45.0%
GTCTCTGCACTCAAATTGGA+AGG - chr1.4:68883504-68883523 MS.gene21032:intron 45.0%
TACTTGGAAGTCACCAGAAG+AGG - chr1.4:68883192-68883211 MS.gene21032:intron 45.0%
TCAGATGCCAAACTCACCAA+TGG - chr1.4:68882419-68882438 MS.gene21032:CDS 45.0%
TCCCTTCTTCCCAGTTGATT+TGG - chr1.4:68884995-68885014 MS.gene21032:CDS 45.0%
TGATATCCACTTTGTCCGGT+AGG + chr1.4:68884490-68884509 None:intergenic 45.0%
TGGATAACAGGGCAAATACC+TGG + chr1.4:68883227-68883246 None:intergenic 45.0%
TGGCAAAAAAGTCCTACGCA+TGG - chr1.4:68884564-68884583 MS.gene21032:intron 45.0%
TTCTACAGGTTTCCTGCAAC+CGG - chr1.4:68883600-68883619 MS.gene21032:intron 45.0%
TTCTGTCAACAATGCAGCTG+TGG - chr1.4:68884935-68884954 MS.gene21032:CDS 45.0%
TTGGCATCTGAAGGTGATGA+TGG + chr1.4:68882410-68882429 None:intergenic 45.0%
TTGGTGAGTTTGGCATCTGA+AGG + chr1.4:68882419-68882438 None:intergenic 45.0%
! ATTGCCACTTCCTGCATGTT+TGG - chr1.4:68882692-68882711 MS.gene21032:intron 45.0%
!! GGGCACTGAAGATCTTAAGA+AGG - chr1.4:68884815-68884834 MS.gene21032:intron 45.0%
!!! ACTGAAGTTTTGGACTCCTC+TGG - chr1.4:68882512-68882531 MS.gene21032:CDS 45.0%
!!! CTGAAGTTTTGGACTCCTCT+GGG - chr1.4:68882513-68882532 MS.gene21032:CDS 45.0%
AAGCATCAACAGCCATGCGT+AGG + chr1.4:68884579-68884598 None:intergenic 50.0%
AGGAAGCAGAACTCGACTAC+TGG - chr1.4:68883968-68883987 MS.gene21032:intron 50.0%
CAACAATGCAGCTGTGGAGT+TGG - chr1.4:68884941-68884960 MS.gene21032:CDS 50.0%
CTGGTGAAAACCTCCTCTTC+TGG + chr1.4:68883208-68883227 None:intergenic 50.0%
GAAAAGAGGCCTAGTGTGTG+CGG - chr1.4:68884870-68884889 MS.gene21032:intron 50.0%
GCTCAAGATCCGGACATTGA+TGG - chr1.4:68884544-68884563 MS.gene21032:intron 50.0%
TACGCATGGCTGTTGATGCT+TGG - chr1.4:68884578-68884597 MS.gene21032:intron 50.0%
TAGTCGTAACCGCACACACT+AGG + chr1.4:68884882-68884901 None:intergenic 50.0%
TATCCACTTTGTCCGGTAGG+AGG + chr1.4:68884487-68884506 None:intergenic 50.0%
TTGGAAGTCACCAGAAGAGG+AGG - chr1.4:68883195-68883214 MS.gene21032:intron 50.0%
! CAGAAGAGGAGGTTTTCACC+AGG - chr1.4:68883206-68883225 MS.gene21032:intron 50.0%
! TTAGATACCTTGGGCGCTGC+CGG + chr1.4:68883622-68883641 None:intergenic 55.0%
AAACGGCGAGGTCCTCCTAC+CGG - chr1.4:68884472-68884491 MS.gene21032:intron 60.0%
AGCCGGCTGTCAAGAACCTC+GGG - chr1.4:68884390-68884409 MS.gene21032:intron 60.0%
CACCCGAGGTTCTTGACAGC+CGG + chr1.4:68884395-68884414 None:intergenic 60.0%
GAGCCGGCTGTCAAGAACCT+CGG - chr1.4:68884389-68884408 MS.gene21032:intron 60.0%
GGTCCTCCTACCGGACAAAG+TGG - chr1.4:68884481-68884500 MS.gene21032:intron 60.0%
!! GGTGCTTGTAGAAGCACCCG+AGG + chr1.4:68884409-68884428 None:intergenic 60.0%
CCTGCAACCGGCAGCGCCCA+AGG - chr1.4:68883612-68883631 MS.gene21032:intron 75.0%
! CCTTGGGCGCTGCCGGTTGC+AGG + chr1.4:68883615-68883634 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 68882383 68885061 68882383 ID=MS.gene21032
chr1.4 mRNA 68882383 68885061 68882383 ID=MS.gene21032.t1;Parent=MS.gene21032
chr1.4 exon 68884898 68885061 68884898 ID=MS.gene21032.t1.exon1;Parent=MS.gene21032.t1
chr1.4 CDS 68884898 68885061 68884898 ID=cds.MS.gene21032.t1;Parent=MS.gene21032.t1
chr1.4 exon 68884217 68884292 68884217 ID=MS.gene21032.t1.exon2;Parent=MS.gene21032.t1
chr1.4 CDS 68884217 68884292 68884217 ID=cds.MS.gene21032.t1;Parent=MS.gene21032.t1
chr1.4 exon 68883811 68883970 68883811 ID=MS.gene21032.t1.exon3;Parent=MS.gene21032.t1
chr1.4 CDS 68883811 68883970 68883811 ID=cds.MS.gene21032.t1;Parent=MS.gene21032.t1
chr1.4 exon 68883377 68883487 68883377 ID=MS.gene21032.t1.exon4;Parent=MS.gene21032.t1
chr1.4 CDS 68883377 68883487 68883377 ID=cds.MS.gene21032.t1;Parent=MS.gene21032.t1
chr1.4 exon 68882817 68883058 68882817 ID=MS.gene21032.t1.exon5;Parent=MS.gene21032.t1
chr1.4 CDS 68882817 68883058 68882817 ID=cds.MS.gene21032.t1;Parent=MS.gene21032.t1
chr1.4 exon 68882383 68882700 68882383 ID=MS.gene21032.t1.exon6;Parent=MS.gene21032.t1
chr1.4 CDS 68882383 68882700 68882383 ID=cds.MS.gene21032.t1;Parent=MS.gene21032.t1
Gene Sequence

>MS.gene21032

ATGGAAGGCAACAAAACTATGGTTCCATCATCACCTTCAGATGCCAAACTCACCAATGGAATTGTGTCCAATGGGAAACAGTACTCTTCTCCTGATGACTTAGAGATAGAGACCTTCTATGTCAAACTAACTGAAGTTTTGGACTCCTCTGGGTTCAATCTCATGTCTGTTTCTTCAATCAGCTTCTTACTTTCTGTTTTGCTTTTATTTTTTTCGTTTGTATTTGCATGAGTGTTTTTACTTGTGTGCATTATGCTATGTTTGGTTTTACAAAATTTGCATGCAAAAAACGTCAAGCATTTTTTTTTAATTGCCACTTCCTGCATGTTTGGATATTTGGATTTACGGTAAATTTGTAAAAATAACAGTGACATACTCGATTTTATTCTCCAATTTGTAGTTTTTGCCACAAATTCATCCAAACATGCACTAAAAGAAAAAGGAAAAAGTGTGTGTACTTGTGGATAAAAAATGTAGTTGGAGAAGGTCACTAGCTAGTCATAGTCATCGGCAAAGTCACCACAAAAGAAAAATCGAGACATGCATTTAGTCATATTGGTCCTTAAAACTGAAATGTGTTGTAATTTGTTCCTCTTTAAATGTTTGGTATAAAGAATGTCACTAGAATAGTAGAATGTGCTTCAATTTAGAAGGGAAAGGTGATGTGATACATGAAACATGAAATGTTATTGACTTTATTTTATATAAAGTTTTATGAGATAATAAAGGGTATATGACCATGCACCTCTGTTTCAATTCTACTTGACAGTTTCAATGTCCGAGAGACAATCTTAGACTTGTACCTGTTTTACTTGGAAGTCACCAGAAGAGGAGGTTTTCACCAGGTATTTGCCCTGTTATCCACATATTGACCTCCCATGATCACATGATTTAATTTGTTGTATTTGTTATATGTTCACTTCTCATTAATGCATGTGGCTTCTATTATTTGCATGCACACCAAGTCTATCGATCCAATATGTTGTGTCTATATAATATAAATTAGTCAAGAGAGGCTTTTAATTAAGTAATTTTTCATATGAATTGTTCAGATTTAATATATTGTAATGGATTCTCTTGAATTTGTCCAGGTTGGTCTTCAAAACAAATGGGCTGAAGTTGTCTCTGCACTCAAATTGGAAGGCAACAATGCAATGTTATCTCCTCAAGTTGAAAAGATCTATGCAACACTTCTATACAAATTTGAGAAATTGTACTTCTACAGGTTTCCTGCAACCGGCAGCGCCCAAGGTATCTAAGAGATCAATTTGTGGTTATTTTAAGTAGATATCTTGTTTCATTCTGGTACTTCACTTAGATGCTTGATATATAGATATTTGGTAGTTTATACATAATATGATTTTAATTTACAACTAGTAATAGAAAAAAGCCTTGTAAAAAACATTGGTCTAGGAAAATGTATTGGATATGAAATGAAATTAAATCACTCGGTGGTGACACAAGTTTACATCGGATCATTGTATGATGTTTTTATTACATTTTTGTTTTAAGATGAAAATGTGTTTAACCTTAAAGTCTCCTTTCTTTTCTCCTCACACCTACATTTTGTAAAGGTACGCGTAAAAGGAAGCAGAACTCGACTACTGGTTTATCTCAATTAATGGCTGATGAAGATAATCTGAATGTGGCAAAGGTTTCCAAGGACTACTCTAGCCAAATGACAGGTTATAAGGATTTAGAATATTTATGCAATGAAAATTTGATCCACTGATATGTAAAAGGTTGCACTCAAGTACTAATGAATTAACTAGCTAACTAGCTGAATCAAAACTAATATCACAACATAACACAATTTATCAACTAATCATGATAACTGAGATTACTAAAGTTCCAAAACAGGTTTGTTACTTTATGTACTTATTAGCTGCAATGTGGACATTTTTTTTCTTTAGTAACTCCAAGTGATTTGTTAAATGAGTTTAAAGGTGTATCTTGTGAATTTAGAGGTGCTTATGCTGCAATATTTGGATTCTTAATTAAAGCAGGAGCCGGCTGTCAAGAACCTCGGGTGCTTCTACAAGCACCCTCAAATGACAAAGAAAACAAGAAACAAGACAAAGAAAAGAAGAAACGGCGAGGTCCTCCTACCGGACAAAGTGGATATCAAATATTCCTCAAGCATGAATGTGCTCGATTAAAAGCTCAAGATCCGGACATTGATGGCAAAAAAGTCCTACGCATGGCTGTTGATGCTTGGCAGAAGATGTCTGCCACTGAAAAACAGGTATGATAAATAAAATTGACATAATATCGATCAAATATCGAACAATTTATATTTTTGCAATTAGCCTCTACTTTGTTTGTTTGAAATTTTCCATTTATAATTACTTTTTCATGTAGCCATATGTGGAGGAAAGCAAGAAGATAAAGGAAAAAAAGAAGGAAGCAGTGATCATTGACAATAAACAGATGGGCACTGAAGATCTTAAGAAGGATGAAAAGAAGAGCACTCAAGATCTTAAGGAGGAAAAGAGGCCTAGTGTGTGCGGTTACGACTACTGTGCAACTTCGCAACCACTACCAAACTATTCTTCTGTCAACAATGCAGCTGTGGAGTTGGCTTTGAAAATGACTGAAAAAGCACTCAAGGATCCCTTCTTCCCAGTTGATTTGGATACTTACTGTTCGTTGGATATGTTAAATGGGAAATCCAAATGA
Protein sequence

>MS.gene21032.t1

MEGNKTMVPSSPSDAKLTNGIVSNGKQYSSPDDLEIETFYVKLTEVLDSSGFNLIFNVRETILDLYLFYLEVTRRGGFHQVGLQNKWAEVVSALKLEGNNAMLSPQVEKIYATLLYKFEKLYFYRFPATGSAQGTRKRKQNSTTGLSQLMADEDNLNVAKVSKDYSSQMTAGAGCQEPRVLLQAPSNDKENKKQDKEKKKRRGPPTGQSGYQIFLKHECARLKAQDPDIDGKKVLRMAVDAWQKMSATEKQPYVEESKKIKEKKKEAVIIDNKQMGTEDLKKDEKKSTQDLKEEKRPSVCGYDYCATSQPLPNYSSVNNAAVELALKMTEKALKDPFFPVDLDTYCSLDMLNGKSK