AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21216

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21216.t1 MTR_5g013010 98.311 296 5 0 1 296 1 296 0.0 598
MS.gene21216.t1 MTR_4g100550 72.333 300 54 9 1 293 2 279 3.26e-138 392
MS.gene21216.t1 MTR_5g013010 98.930 187 2 0 1 187 1 187 3.81e-134 379
MS.gene21216.t1 MTR_8g006705 56.958 309 101 10 4 288 3 303 3.81e-94 281
MS.gene21216.t1 MTR_7g093430 64.516 186 58 4 72 250 89 273 1.22e-67 213
MS.gene21216.t1 MTR_1g054285 60.204 196 54 4 71 251 82 268 1.44e-66 210
MS.gene21216.t1 MTR_2g061030 73.026 152 35 4 112 260 175 323 1.48e-65 209
MS.gene21216.t1 MTR_4g097600 78.049 123 26 1 127 249 118 239 7.14e-61 194
MS.gene21216.t1 MTR_5g014890 65.972 144 46 2 127 268 114 256 5.70e-60 192
MS.gene21216.t1 MTR_1g017090 51.667 180 73 5 96 263 88 265 1.51e-51 171
MS.gene21216.t1 MTR_4g084750 62.097 124 42 1 130 248 59 182 2.48e-45 152
MS.gene21216.t1 MTR_2g038625 57.627 118 50 0 136 253 82 199 3.93e-41 142
MS.gene21216.t1 MTR_2g038580 57.627 118 50 0 136 253 82 199 3.93e-41 142
MS.gene21216.t1 MTR_3g103590 65.217 115 38 2 135 249 144 256 5.91e-39 139
MS.gene21216.t1 MTR_4g126900 61.404 114 41 1 138 248 90 203 1.52e-38 135
MS.gene21216.t1 MTR_7g010020 42.537 134 63 3 97 225 49 173 3.28e-20 88.2
MS.gene21216.t1 MTR_5g038280 39.535 172 77 6 78 228 20 185 2.05e-19 87.0
MS.gene21216.t1 MTR_8g468210 41.463 123 70 2 100 222 43 163 7.67e-19 84.7
MS.gene21216.t1 MTR_6g007647 43.590 117 65 1 136 252 33 148 1.01e-18 84.0
MS.gene21216.t1 MTR_6g007647 43.590 117 65 1 136 252 67 182 2.01e-18 83.6
MS.gene21216.t1 MTR_4g107650 52.632 95 38 1 134 221 93 187 6.27e-18 83.2
MS.gene21216.t1 MTR_6g011610 31.401 207 106 5 18 223 12 183 6.99e-18 82.0
MS.gene21216.t1 MTR_5g039000 39.516 124 75 0 100 223 41 164 1.45e-17 81.3
MS.gene21216.t1 MTR_6g011610 46.000 100 53 1 125 223 65 164 2.44e-17 80.1
MS.gene21216.t1 MTR_1g061660 47.191 89 47 0 136 224 59 147 4.07e-17 79.0
MS.gene21216.t1 MTR_3g080100 38.926 149 65 4 97 223 9 153 4.32e-17 79.3
MS.gene21216.t1 MTR_3g080100 48.421 95 42 1 136 223 20 114 4.63e-17 78.6
MS.gene21216.t1 MTR_7g103340 45.455 88 48 0 136 223 63 150 1.21e-16 77.8
MS.gene21216.t1 MTR_3g086790 40.625 128 68 2 128 247 52 179 2.82e-16 77.8
MS.gene21216.t1 MTR_8g089895 47.475 99 45 1 132 223 50 148 3.03e-16 77.8
MS.gene21216.t1 MTR_8g469430 46.809 94 43 1 136 222 89 182 1.53e-15 75.5
MS.gene21216.t1 MTR_8g026960 42.683 82 47 0 140 221 38 119 1.28e-11 63.5
MS.gene21216.t1 MTR_5g029320 31.690 142 91 1 80 221 42 177 2.94e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21216.t1 AT4G16780 65.101 298 88 7 1 296 1 284 5.42e-109 318
MS.gene21216.t1 AT5G47370 59.936 312 80 13 1 296 1 283 7.53e-107 312
MS.gene21216.t1 AT4G17460 59.032 310 85 11 1 296 1 282 2.82e-105 308
MS.gene21216.t1 AT3G60390 59.060 298 84 14 17 287 23 309 1.29e-91 275
MS.gene21216.t1 AT2G44910 53.165 316 115 12 4 288 3 316 3.08e-84 256
MS.gene21216.t1 AT5G06710 61.421 197 64 6 91 277 135 329 2.82e-67 213
MS.gene21216.t1 AT4G37790 59.358 187 57 5 105 273 86 271 3.45e-62 198
MS.gene21216.t1 AT2G22800 72.308 130 32 2 123 249 97 225 2.61e-58 188
MS.gene21216.t1 AT2G01430 52.299 174 65 4 92 256 95 259 5.35e-44 151
MS.gene21216.t1 AT1G70920 60.976 123 37 2 135 257 68 179 8.61e-38 133
MS.gene21216.t1 AT1G70920 60.976 123 37 2 135 257 38 149 8.86e-38 132
MS.gene21216.t1 AT5G06710 58.879 107 34 4 91 187 135 241 4.50e-27 107
MS.gene21216.t1 AT1G69780 41.221 131 67 2 98 223 47 172 4.84e-20 88.2
MS.gene21216.t1 AT2G01430 47.059 119 49 3 92 204 95 205 5.72e-19 84.0
MS.gene21216.t1 AT1G26960 46.341 123 61 4 103 223 39 158 1.75e-18 83.2
MS.gene21216.t1 AT3G01470 48.387 93 41 1 136 221 68 160 6.06e-17 79.3
MS.gene21216.t1 AT5G03790 37.313 134 83 1 136 269 77 209 4.37e-16 76.3
MS.gene21216.t1 AT5G15150 38.983 118 65 1 106 223 92 202 2.60e-15 75.5
MS.gene21216.t1 AT1G27045 47.748 111 45 3 127 224 53 163 3.59e-15 73.9
MS.gene21216.t1 AT3G01220 45.745 94 47 1 136 225 87 180 4.90e-15 74.3
MS.gene21216.t1 AT1G27045 54.321 81 31 1 127 201 53 133 9.23e-15 72.4
MS.gene21216.t1 AT1G27045 59.091 66 27 0 136 201 17 82 1.01e-14 71.2
MS.gene21216.t1 AT1G27045 59.091 66 27 0 136 201 25 90 1.06e-14 71.2
MS.gene21216.t1 AT1G27045 59.091 66 27 0 136 201 25 90 1.06e-14 71.2
MS.gene21216.t1 AT5G65310 35.417 144 84 3 133 269 69 210 1.84e-14 72.8
MS.gene21216.t1 AT4G40060 44.330 97 47 1 134 223 57 153 1.93e-14 72.8
MS.gene21216.t1 AT5G65310 35.417 144 84 3 133 269 51 192 2.37e-14 72.4
MS.gene21216.t1 AT2G22430 44.792 96 49 1 134 225 60 155 4.45e-14 71.6
MS.gene21216.t1 AT5G03790 43.956 91 51 0 136 226 77 167 3.90e-13 67.4
MS.gene21216.t1 AT2G46680 41.053 95 52 1 139 229 35 129 4.81e-13 68.2
MS.gene21216.t1 AT2G46680 40.909 88 52 0 139 226 35 122 5.33e-13 67.8
MS.gene21216.t1 AT2G36610 39.796 98 51 1 135 224 70 167 1.13e-12 65.9
MS.gene21216.t1 AT4G36740 43.678 87 49 0 136 222 55 141 6.21e-12 64.3
MS.gene21216.t1 AT4G36740 43.678 87 49 0 136 222 56 142 6.36e-12 64.3
MS.gene21216.t1 AT5G53980 42.391 92 53 0 131 222 6 97 4.42e-11 60.5
MS.gene21216.t1 AT2G18550 43.678 87 49 0 136 222 61 147 6.03e-11 61.6

Find 63 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTATTGAAAAGGAAGATTT+TGG 0.211828 5.1:+6093399 MS.gene21216:CDS
AGATTAAGGTATTGAGGATT+TGG 0.288551 5.1:-6093456 None:intergenic
GAGAATAATTGATTGGTCTT+TGG 0.289348 5.1:-6094028 None:intergenic
GGAAATGGACGTGGGTGATT+TGG 0.317565 5.1:-6094803 None:intergenic
TTGAAGAAGACATAAGATTA+AGG 0.336048 5.1:-6093470 None:intergenic
ATTGGCACTTGCAAAACAAT+TGG 0.337338 5.1:+6094184 MS.gene21216:CDS
GCCCATGGTCCCACAGGAAA+TGG 0.346020 5.1:-6094818 None:intergenic
TTTGAGGGAAGCTCAAACTT+AGG 0.385146 5.1:-6093428 None:intergenic
GTGCTAGACAAGTTGAAGTT+TGG 0.390504 5.1:+6094213 MS.gene21216:CDS
AAGAAGACGCTGAAACTTCA+AGG 0.402562 5.1:+6093989 MS.gene21216:CDS
TCTCTTCGAGAATAATTGAT+TGG 0.404047 5.1:-6094035 None:intergenic
GACATAAGATTAAGGTATTG+AGG 0.421167 5.1:-6093462 None:intergenic
GATGTGAATCGGTTACCTTC+TGG 0.421492 5.1:+6093829 MS.gene21216:CDS
TTTACATGCAGATCGGGATT+CGG 0.433835 5.1:+6093762 MS.gene21216:intron
ACACTTGAAACGGTGCTGTT+TGG 0.443597 5.1:-6093886 None:intergenic
TTGGCACTTGCAAAACAATT+GGG 0.452760 5.1:+6094185 MS.gene21216:CDS
GGGCACATGGTAAGAGTTGT+TGG 0.457834 5.1:-6094713 None:intergenic
GTCCATTTCCTGTGGGACCA+TGG 0.458770 5.1:+6094816 MS.gene21216:CDS
TGAAAATAGACGGTTACAAA+AGG 0.474850 5.1:+6094631 MS.gene21216:CDS
TTTACCACTCACACTTGAAA+CGG 0.484021 5.1:-6093896 None:intergenic
TCCATTTCCTGTGGGACCAT+GGG 0.490692 5.1:+6094817 MS.gene21216:CDS
TTAAGGTATTGAGGATTTGG+TGG 0.491711 5.1:-6093453 None:intergenic
AGAAGCGAAAGAGAAGTTAC+AGG 0.494456 5.1:+6093919 MS.gene21216:CDS
GCAGCATCAACGGCAGATGA+TGG 0.502736 5.1:-6094758 None:intergenic
ATTGAAAGTAGAAGGAGAAG+AGG 0.503918 5.1:-6093499 None:intergenic
ATCCGTACAAGTTAAGATCT+AGG 0.515404 5.1:-6094884 None:intergenic
TCATTTGCATGTAGAATTGT+GGG 0.520585 5.1:-6094685 None:intergenic
GTCATTTGCATGTAGAATTG+TGG 0.521859 5.1:-6094686 None:intergenic
TCGATTCCTCGGAGAAGTAA+AGG 0.525636 5.1:-6093808 None:intergenic
TCCGAGGAATCGATGTGAAT+CGG 0.527394 5.1:+6093818 MS.gene21216:CDS
GCTGTGGCCCATGGTCCCAC+AGG 0.538822 5.1:-6094824 None:intergenic
TCAAAGGTCCTATGTTGGAT+AGG 0.542840 5.1:-6094851 None:intergenic
ACGACGCGTGGCAGCATCAA+CGG 0.548952 5.1:-6094768 None:intergenic
ATGGAAAGAGATTGTTCAAG+AGG 0.550270 5.1:+6093955 MS.gene21216:CDS
TGCCACGCGTCGTCATCCTA+TGG 0.554799 5.1:+6094778 MS.gene21216:CDS
ACCGATTCACATCGATTCCT+CGG 0.556594 5.1:-6093819 None:intergenic
CGTGGGTGATTTGGAGCCAT+AGG 0.560215 5.1:-6094794 None:intergenic
CACATGGTAAGAGTTGTTGG+TGG 0.564954 5.1:-6094710 None:intergenic
ATAGGAGCAGCTGTGGCCCA+TGG 0.566808 5.1:-6094833 None:intergenic
GGTCCCACAGGAAATGGACG+TGG 0.582860 5.1:-6094812 None:intergenic
GGTTTCAAAATCGTAGAGCA+AGG 0.583437 5.1:+6094234 MS.gene21216:CDS
ACAGCAACACGCTCACAAGA+GGG 0.586849 5.1:-6094734 None:intergenic
ATGTTGGATAGGAGCAGCTG+TGG 0.588541 5.1:-6094840 None:intergenic
CAGCTGCTCCTATCCAACAT+AGG 0.588914 5.1:+6094843 MS.gene21216:CDS
GTGTATCAAAGGTCCTATGT+TGG 0.591308 5.1:-6094856 None:intergenic
CACCCACGTCCATTTCCTGT+GGG 0.600865 5.1:+6094809 MS.gene21216:CDS
AACAGCAACACGCTCACAAG+AGG 0.612400 5.1:-6094735 None:intergenic
ATCTAACGGATGAAAATAGA+CGG 0.614300 5.1:+6094621 MS.gene21216:CDS
TCACCCACGTCCATTTCCTG+TGG 0.615645 5.1:+6094808 MS.gene21216:CDS
TCTAGGACGGAGTGTATCAA+AGG 0.615968 5.1:-6094867 None:intergenic
TCTTCACAATCAGCACCAGA+AGG 0.621185 5.1:-6093844 None:intergenic
ACGCTCACAAGAGGGGCACA+TGG 0.624285 5.1:-6094726 None:intergenic
GCTGATTGTGAAGAAGAAGC+AGG 0.645646 5.1:+6093853 MS.gene21216:CDS
GTACAAGTTAAGATCTAGGA+CGG 0.655097 5.1:-6094880 None:intergenic
AGCCATAGGATGACGACGCG+TGG 0.657092 5.1:-6094780 None:intergenic
TACAGGTGAAGATCTTGACA+TGG 0.657130 5.1:+6093936 MS.gene21216:CDS
CAGCAACACGCTCACAAGAG+GGG 0.670133 5.1:-6094733 None:intergenic
AGCACCGTTTCAAGTGTGAG+TGG 0.674395 5.1:+6093892 MS.gene21216:CDS
AGACATGCAGAATCGAAGAA+CGG 0.674956 5.1:+6093785 MS.gene21216:CDS
GAACGGCCTTTACTTCTCCG+AGG 0.691005 5.1:+6093802 MS.gene21216:CDS
GTCCCACAGGAAATGGACGT+GGG 0.703771 5.1:-6094811 None:intergenic
CATTTGCATGTAGAATTGTG+GGG 0.706166 5.1:-6094684 None:intergenic
AAGATGTTGTGAGAATCTAA+CGG 0.732801 5.1:+6094607 MS.gene21216:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TAAATTATATTTTTTTTTTT+AGG + chr5.1:6094119-6094138 MS.gene21216:intron 0.0%
!! CAATATTTATTTATTTATGT+TGG + chr5.1:6094521-6094540 MS.gene21216:intron 10.0%
!!! TAAAAATCAATTTAAGTTAA+TGG - chr5.1:6094455-6094474 None:intergenic 10.0%
!! ATGAAAATGATTGTTAATTA+AGG - chr5.1:6093625-6093644 None:intergenic 15.0%
!! TTACACTTAATTAATACTTA+CGG - chr5.1:6094088-6094107 None:intergenic 15.0%
!!! TATCAATATTTTTTTTGTGA+AGG + chr5.1:6094492-6094511 MS.gene21216:intron 15.0%
!! TACACTTAATTAATACTTAC+GGG - chr5.1:6094087-6094106 None:intergenic 20.0%
!! TATTATTCCAAAGTTGTAAT+TGG - chr5.1:6093595-6093614 None:intergenic 20.0%
!! TTGTTTGTAGAAACAAAAAT+TGG + chr5.1:6094166-6094185 MS.gene21216:intron 20.0%
!!! TTTAAAACTCAAAAGATATC+CGG + chr5.1:6094294-6094313 MS.gene21216:intron 20.0%
! ACACTTAATTAATACTTACG+GGG - chr5.1:6094086-6094105 None:intergenic 25.0%
! GTAAAGGAAGGAAATTATAT+AGG - chr5.1:6093660-6093679 None:intergenic 25.0%
! TAAGATAGATTACAGAGTAA+AGG - chr5.1:6093676-6093695 None:intergenic 25.0%
! TTGAAGAAGACATAAGATTA+AGG - chr5.1:6093473-6093492 None:intergenic 25.0%
!! GTTTGTTAAATTGTGATTTC+AGG + chr5.1:6094543-6094562 MS.gene21216:intron 25.0%
!!! AATGATGTTTTTACTTCTTC+AGG + chr5.1:6093540-6093559 MS.gene21216:CDS 25.0%
!!! AGTATTGAAAAGGAAGATTT+TGG + chr5.1:6093399-6093418 MS.gene21216:CDS 25.0%
!!! ATCTTATTTTACATGCAGAT+CGG + chr5.1:6093755-6093774 MS.gene21216:intron 25.0%
!!! TTGACTAGTCTTTATTTAGT+TGG + chr5.1:6094266-6094285 MS.gene21216:intron 25.0%
AAGATGTTGTGAGAATCTAA+CGG + chr5.1:6094607-6094626 MS.gene21216:CDS 30.0%
ATAGATTACAGAGTAAAGGA+AGG - chr5.1:6093672-6093691 None:intergenic 30.0%
GTAAAAACATCATTCCAAGA+AGG - chr5.1:6093534-6093553 None:intergenic 30.0%
TCATTTGCATGTAGAATTGT+GGG - chr5.1:6094688-6094707 None:intergenic 30.0%
TCTCTTCGAGAATAATTGAT+TGG - chr5.1:6094038-6094057 None:intergenic 30.0%
TGAAAATAGACGGTTACAAA+AGG + chr5.1:6094631-6094650 MS.gene21216:CDS 30.0%
! ATCTAACGGATGAAAATAGA+CGG + chr5.1:6094621-6094640 MS.gene21216:CDS 30.0%
!! AGATTAAGGTATTGAGGATT+TGG - chr5.1:6093459-6093478 None:intergenic 30.0%
!! GACATAAGATTAAGGTATTG+AGG - chr5.1:6093465-6093484 None:intergenic 30.0%
!! GAGAATAATTGATTGGTCTT+TGG - chr5.1:6094031-6094050 None:intergenic 30.0%
!! TTTTGAGGATTGAAAGTAGA+AGG - chr5.1:6093510-6093529 None:intergenic 30.0%
!!! TCTTATTTTACATGCAGATC+GGG + chr5.1:6093756-6093775 MS.gene21216:intron 30.0%
ATCTGGTCCAATTACAACTT+TGG + chr5.1:6093585-6093604 MS.gene21216:intron 35.0%
ATGGAAAGAGATTGTTCAAG+AGG + chr5.1:6093955-6093974 MS.gene21216:CDS 35.0%
ATTGAAAGTAGAAGGAGAAG+AGG - chr5.1:6093502-6093521 None:intergenic 35.0%
ATTGGCACTTGCAAAACAAT+TGG + chr5.1:6094184-6094203 MS.gene21216:CDS 35.0%
CATTTGCATGTAGAATTGTG+GGG - chr5.1:6094687-6094706 None:intergenic 35.0%
GTCATTTGCATGTAGAATTG+TGG - chr5.1:6094689-6094708 None:intergenic 35.0%
TCAATCCTCAAAAACCTTCT+TGG + chr5.1:6093517-6093536 MS.gene21216:CDS 35.0%
TTGGCACTTGCAAAACAATT+GGG + chr5.1:6094185-6094204 MS.gene21216:CDS 35.0%
TTTACCACTCACACTTGAAA+CGG - chr5.1:6093899-6093918 None:intergenic 35.0%
TTTCATGTTTGGTATCACGT+TGG + chr5.1:6094416-6094435 MS.gene21216:intron 35.0%
! GAGATGCATGTTTTCATGTT+TGG + chr5.1:6094405-6094424 MS.gene21216:intron 35.0%
! TCATTCCAAGAAGGTTTTTG+AGG - chr5.1:6093525-6093544 None:intergenic 35.0%
!! TTAAGGTATTGAGGATTTGG+TGG - chr5.1:6093456-6093475 None:intergenic 35.0%
AAGAAGACGCTGAAACTTCA+AGG + chr5.1:6093989-6094008 MS.gene21216:CDS 40.0%
AGAAGCGAAAGAGAAGTTAC+AGG + chr5.1:6093919-6093938 MS.gene21216:CDS 40.0%
AGACATGCAGAATCGAAGAA+CGG + chr5.1:6093785-6093804 MS.gene21216:CDS 40.0%
ATCCTTCCATTCTCTAGATC+TGG + chr5.1:6093568-6093587 MS.gene21216:intron 40.0%
ATTGGACCAGATCTAGAGAA+TGG - chr5.1:6093577-6093596 None:intergenic 40.0%
GGTTTCAAAATCGTAGAGCA+AGG + chr5.1:6094234-6094253 MS.gene21216:CDS 40.0%
GTGTATCAAAGGTCCTATGT+TGG - chr5.1:6094859-6094878 None:intergenic 40.0%
TACAGGTGAAGATCTTGACA+TGG + chr5.1:6093936-6093955 MS.gene21216:CDS 40.0%
TCAAAGGTCCTATGTTGGAT+AGG - chr5.1:6094854-6094873 None:intergenic 40.0%
TTTACATGCAGATCGGGATT+CGG + chr5.1:6093762-6093781 MS.gene21216:intron 40.0%
TTTGAGGGAAGCTCAAACTT+AGG - chr5.1:6093431-6093450 None:intergenic 40.0%
! GTGCTAGACAAGTTGAAGTT+TGG + chr5.1:6094213-6094232 MS.gene21216:CDS 40.0%
!!! GAGGATTTGGTGGATTTTGA+GGG - chr5.1:6093446-6093465 None:intergenic 40.0%
!!! TGAGGATTTGGTGGATTTTG+AGG - chr5.1:6093447-6093466 None:intergenic 40.0%
AAAGATATCCGGTGCATGTC+TGG + chr5.1:6094305-6094324 MS.gene21216:intron 45.0%
CACATGGTAAGAGTTGTTGG+TGG - chr5.1:6094713-6094732 None:intergenic 45.0%
GACCAGATCTAGAGAATGGA+AGG - chr5.1:6093573-6093592 None:intergenic 45.0%
GATGTGAATCGGTTACCTTC+TGG + chr5.1:6093829-6093848 MS.gene21216:CDS 45.0%
GCTGATTGTGAAGAAGAAGC+AGG + chr5.1:6093853-6093872 MS.gene21216:CDS 45.0%
TCCGAGGAATCGATGTGAAT+CGG + chr5.1:6093818-6093837 MS.gene21216:CDS 45.0%
TCGATTCCTCGGAGAAGTAA+AGG - chr5.1:6093811-6093830 None:intergenic 45.0%
TCTTCACAATCAGCACCAGA+AGG - chr5.1:6093847-6093866 None:intergenic 45.0%
! TCTAGGACGGAGTGTATCAA+AGG - chr5.1:6094870-6094889 None:intergenic 45.0%
!! ACACTTGAAACGGTGCTGTT+TGG - chr5.1:6093889-6093908 None:intergenic 45.0%
!! ACCGATTCACATCGATTCCT+CGG - chr5.1:6093822-6093841 None:intergenic 45.0%
AACAGCAACACGCTCACAAG+AGG - chr5.1:6094738-6094757 None:intergenic 50.0%
ACAGCAACACGCTCACAAGA+GGG - chr5.1:6094737-6094756 None:intergenic 50.0%
ATGTTGGATAGGAGCAGCTG+TGG - chr5.1:6094843-6094862 None:intergenic 50.0%
CAGCTGCTCCTATCCAACAT+AGG + chr5.1:6094843-6094862 MS.gene21216:CDS 50.0%
CTGTGATACCAGACATGCAC+CGG - chr5.1:6094316-6094335 None:intergenic 50.0%
GGAAATGGACGTGGGTGATT+TGG - chr5.1:6094806-6094825 None:intergenic 50.0%
GGGCACATGGTAAGAGTTGT+TGG - chr5.1:6094716-6094735 None:intergenic 50.0%
TCCATTTCCTGTGGGACCAT+GGG + chr5.1:6094817-6094836 MS.gene21216:CDS 50.0%
! AGCACCGTTTCAAGTGTGAG+TGG + chr5.1:6093892-6093911 MS.gene21216:CDS 50.0%
CACCCACGTCCATTTCCTGT+GGG + chr5.1:6094809-6094828 MS.gene21216:CDS 55.0%
CAGCAACACGCTCACAAGAG+GGG - chr5.1:6094736-6094755 None:intergenic 55.0%
GAACGGCCTTTACTTCTCCG+AGG + chr5.1:6093802-6093821 MS.gene21216:CDS 55.0%
GCAGCATCAACGGCAGATGA+TGG - chr5.1:6094761-6094780 None:intergenic 55.0%
GTCCATTTCCTGTGGGACCA+TGG + chr5.1:6094816-6094835 MS.gene21216:CDS 55.0%
GTCCCACAGGAAATGGACGT+GGG - chr5.1:6094814-6094833 None:intergenic 55.0%
TCACCCACGTCCATTTCCTG+TGG + chr5.1:6094808-6094827 MS.gene21216:CDS 55.0%
! CGTGGGTGATTTGGAGCCAT+AGG - chr5.1:6094797-6094816 None:intergenic 55.0%
ACGACGCGTGGCAGCATCAA+CGG - chr5.1:6094771-6094790 None:intergenic 60.0%
AGCCATAGGATGACGACGCG+TGG - chr5.1:6094783-6094802 None:intergenic 60.0%
ATAGGAGCAGCTGTGGCCCA+TGG - chr5.1:6094836-6094855 None:intergenic 60.0%
GCCCATGGTCCCACAGGAAA+TGG - chr5.1:6094821-6094840 None:intergenic 60.0%
GGTCCCACAGGAAATGGACG+TGG - chr5.1:6094815-6094834 None:intergenic 60.0%
TGCCACGCGTCGTCATCCTA+TGG + chr5.1:6094778-6094797 MS.gene21216:CDS 60.0%
! ACGCTCACAAGAGGGGCACA+TGG - chr5.1:6094729-6094748 None:intergenic 60.0%
GCTGTGGCCCATGGTCCCAC+AGG - chr5.1:6094827-6094846 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 6093396 6094895 6093396 ID=MS.gene21216
chr5.1 mRNA 6093396 6094895 6093396 ID=MS.gene21216.t1;Parent=MS.gene21216
chr5.1 exon 6093396 6093561 6093396 ID=MS.gene21216.t1.exon1;Parent=MS.gene21216.t1
chr5.1 CDS 6093396 6093561 6093396 ID=cds.MS.gene21216.t1;Parent=MS.gene21216.t1
chr5.1 exon 6093773 6094086 6093773 ID=MS.gene21216.t1.exon2;Parent=MS.gene21216.t1
chr5.1 CDS 6093773 6094086 6093773 ID=cds.MS.gene21216.t1;Parent=MS.gene21216.t1
chr5.1 exon 6094176 6094255 6094176 ID=MS.gene21216.t1.exon3;Parent=MS.gene21216.t1
chr5.1 CDS 6094176 6094255 6094176 ID=cds.MS.gene21216.t1;Parent=MS.gene21216.t1
chr5.1 exon 6094565 6094895 6094565 ID=MS.gene21216.t1.exon4;Parent=MS.gene21216.t1
chr5.1 CDS 6094565 6094895 6094565 ID=cds.MS.gene21216.t1;Parent=MS.gene21216.t1
Gene Sequence

>MS.gene21216

ATGAGTATTGAAAAGGAAGATTTTGGTTTGAGCCTAAGTTTGAGCTTCCCTCAAAATCCACCAAATCCTCAATACCTTAATCTTATGTCTTCTTCAACTCATTCCTCTTCTCCTTCTACTTTCAATCCTCAAAAACCTTCTTGGAATGATGTTTTTACTTCTTCAGGTGTGTATCCTTCCATTCTCTAGATCTGGTCCAATTACAACTTTGGAATAATAATCTCAACCTTAATTAACAATCATTTTCATATAAAGAAGTTTCCTATATAATTTCCTTCCTTTACTCTGTAATCTATCTTATTTTAATTTCTTCATATGAAATGAAAAGAATCATTTTAATTTCTTCAATTTAATGAACGATCTTATTTTACATGCAGATCGGGATTCGGAGACATGCAGAATCGAAGAACGGCCTTTACTTCTCCGAGGAATCGATGTGAATCGGTTACCTTCTGGTGCTGATTGTGAAGAAGAAGCAGGAGTTTCATCACCAAACAGCACCGTTTCAAGTGTGAGTGGTAAAAGAAGCGAAAGAGAAGTTACAGGTGAAGATCTTGACATGGAAAGAGATTGTTCAAGAGGAATCAGTGATGAAGAAGACGCTGAAACTTCAAGGAAAAAACTTAGACTCACCAAAGACCAATCAATTATTCTCGAAGAGAGTTTCAAAGAACACAACACTCTTAACCCCGTAAGTATTAATTAAGTGTAATTAAGCTCTTTTAAATTATATTTTTTTTTTTAGGTGATTAACTTTTTTTTGATTTGTTTTGTTTGTAGAAACAAAAATTGGCACTTGCAAAACAATTGGGACTTCGTGCTAGACAAGTTGAAGTTTGGTTTCAAAATCGTAGAGCAAGGTTTGTTCATTTGACTAGTCTTTATTTAGTTGGCAATATTTAAAACTCAAAAGATATCCGGTGCATGTCTGGTATCACAGTTGATTTGTCAAAATCACTATGATCTGATGATATTCACTGTGATTTCGACAAATTCATCGCACACATACGAGATGCATGTTTTCATGTTTGGTATCACGTTGGATTTGACTAAGATCCATTAACTTAAATTGATTTTTAAAAACACAAGTGTCATTTATCAATATTTTTTTTGTGAAGGATTTATCAATATTTATTTATTTATGTTGGTTTGTTAAATTGTGATTTCAGGACTAAGTTGAAGCAAACAGAAGTAGACTGTGAGTTTTTGAAAAGATGTTGTGAGAATCTAACGGATGAAAATAGACGGTTACAAAAGGAAGTGCAAGAGTTAAGAGCATTGAAACTTTCCCCACAATTCTACATGCAAATGACACCACCAACAACTCTTACCATGTGCCCCTCTTGTGAGCGTGTTGCTGTTCCATCATCTGCCGTTGATGCTGCCACGCGTCGTCATCCTATGGCTCCAAATCACCCACGTCCATTTCCTGTGGGACCATGGGCCACAGCTGCTCCTATCCAACATAGGACCTTTGATACACTCCGTCCTAGATCTTAA
Protein sequence

>MS.gene21216.t1

MSIEKEDFGLSLSLSFPQNPPNPQYLNLMSSSTHSSSPSTFNPQKPSWNDVFTSSDRDSETCRIEERPLLLRGIDVNRLPSGADCEEEAGVSSPNSTVSSVSGKRSEREVTGEDLDMERDCSRGISDEEDAETSRKKLRLTKDQSIILEESFKEHNTLNPKQKLALAKQLGLRARQVEVWFQNRRARTKLKQTEVDCEFLKRCCENLTDENRRLQKEVQELRALKLSPQFYMQMTPPTTLTMCPSCERVAVPSSAVDAATRRHPMAPNHPRPFPVGPWATAAPIQHRTFDTLRPRS