Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21305.t1 | KEH28124.1 | 95.9 | 121 | 5 | 0 | 13 | 133 | 1 | 121 | 3.00E-60 | 241.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21305.t1 | Q8H1G0 | 41.9 | 105 | 43 | 2 | 47 | 133 | 28 | 132 | 1.6e-09 | 63.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21305.t1 | A0A072UEB1 | 95.9 | 121 | 5 | 0 | 13 | 133 | 1 | 121 | 2.2e-60 | 241.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene21305.t1 | TF | Tify |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21305.t1 | MTR_5g066510 | 95.868 | 121 | 5 | 0 | 13 | 133 | 1 | 121 | 6.94e-84 | 244 |
| MS.gene21305.t1 | MTR_5g066510 | 95.868 | 121 | 5 | 0 | 13 | 133 | 1 | 121 | 1.22e-82 | 244 |
| MS.gene21305.t1 | MTR_1g061610 | 67.647 | 68 | 21 | 1 | 51 | 118 | 42 | 108 | 1.13e-25 | 94.4 |
| MS.gene21305.t1 | MTR_3g100050 | 67.213 | 61 | 19 | 1 | 58 | 117 | 42 | 102 | 1.52e-19 | 82.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene21305.t1 | AT3G21175 | 44.565 | 92 | 29 | 4 | 50 | 119 | 21 | 112 | 1.51e-12 | 62.4 |
| MS.gene21305.t1 | AT1G51600 | 62.963 | 54 | 18 | 1 | 82 | 133 | 79 | 132 | 1.62e-12 | 63.5 |
| MS.gene21305.t1 | AT1G51600 | 62.963 | 54 | 18 | 1 | 82 | 133 | 79 | 132 | 1.62e-12 | 63.5 |
| MS.gene21305.t1 | AT3G21175 | 41.284 | 109 | 40 | 5 | 50 | 134 | 21 | 129 | 2.73e-12 | 62.8 |
| MS.gene21305.t1 | AT3G21175 | 44.565 | 92 | 29 | 4 | 50 | 119 | 21 | 112 | 4.22e-12 | 62.0 |
Find 39 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGCTTCTCAAATTCTCTCTT+TGG | 0.239601 | 5.1:-51640312 | MS.gene21305:CDS |
| AAACTTTGAAGTGCAAATTC+AGG | 0.278085 | 5.1:+51640102 | None:intergenic |
| TCCAACTCCGATGCTGTTTA+CGG | 0.309806 | 5.1:-51640141 | MS.gene21305:CDS |
| TCGCTTTCGCCGGCGGTGAT+TGG | 0.358806 | 5.1:+51640237 | None:intergenic |
| GTCGATGCCGGCGTCGGGAC+CGG | 0.425728 | 5.1:+51640206 | None:intergenic |
| GGCGAAAGCGATGACGGTTC+CGG | 0.429809 | 5.1:-51640225 | MS.gene21305:CDS |
| CAGCTTACGCTGTCGTTTCG+TGG | 0.436824 | 5.1:-51640066 | MS.gene21305:CDS |
| TCAATCGGTGTTGTTACTGT+TGG | 0.455881 | 5.1:-51639788 | MS.gene21305:CDS |
| CAATCGGTGTTGTTACTGTT+GGG | 0.455920 | 5.1:-51639787 | MS.gene21305:CDS |
| TGAGCGTCGATGCCGGCGTC+GGG | 0.466145 | 5.1:+51640201 | None:intergenic |
| AAATTCTCTCTTTGGTGCAA+TGG | 0.471067 | 5.1:-51640304 | MS.gene21305:CDS |
| CGGTGCCTCAACAAAGTTTG+AGG | 0.475750 | 5.1:-51639732 | MS.gene21305:CDS |
| ATTGAGAATCGAACCTTAGC+AGG | 0.477071 | 5.1:+51640015 | None:intergenic |
| GCCGTAAACAGCATCGGAGT+TGG | 0.479472 | 5.1:+51640140 | None:intergenic |
| GACGGTTCCGGTCCCGACGC+CGG | 0.488975 | 5.1:-51640213 | MS.gene21305:CDS |
| TCAGTACGAAACGCACGGTA+TGG | 0.494591 | 5.1:-51640169 | MS.gene21305:CDS |
| TGACTCTGTTACGCCTGCTA+AGG | 0.495608 | 5.1:-51640028 | MS.gene21305:intron |
| ACCGCATTCACACCAGCATC+AGG | 0.496179 | 5.1:+51639751 | None:intergenic |
| TTTCATGTTACAGGTTCAAT+CGG | 0.502821 | 5.1:-51639803 | MS.gene21305:intron |
| TCCGATGCTGTTTACGGCAA+CGG | 0.517634 | 5.1:-51640135 | MS.gene21305:CDS |
| TTGAGCGTCGATGCCGGCGT+CGG | 0.518484 | 5.1:+51640200 | None:intergenic |
| ATTGGTACTGTGGTGTTCAA+AGG | 0.519474 | 5.1:+51640255 | None:intergenic |
| ACCGTCATCGCTTTCGCCGG+CGG | 0.524563 | 5.1:+51640230 | None:intergenic |
| GGTTGAGAGTGTTCGTACAT+TGG | 0.539771 | 5.1:+51640276 | None:intergenic |
| TGAGAGTGTTCGTACATTGG+TGG | 0.550890 | 5.1:+51640279 | None:intergenic |
| TTGATGTTGAGCGTCGATGC+CGG | 0.559465 | 5.1:+51640194 | None:intergenic |
| GGAACCGTCATCGCTTTCGC+CGG | 0.559788 | 5.1:+51640227 | None:intergenic |
| GGATGTGAACAACCTGATGC+TGG | 0.568670 | 5.1:-51639763 | MS.gene21305:CDS |
| ACCGCCGGCGAAAGCGATGA+CGG | 0.576304 | 5.1:-51640231 | MS.gene21305:CDS |
| GGTGCCTCAACAAAGTTTGA+GGG | 0.580121 | 5.1:-51639731 | MS.gene21305:CDS |
| GCCGTTGCCGTAAACAGCAT+CGG | 0.590158 | 5.1:+51640134 | None:intergenic |
| TCGGTGTTGTTACTGTTGGG+TGG | 0.618046 | 5.1:-51639784 | MS.gene21305:CDS |
| ACCACCCTCAAACTTTGTTG+AGG | 0.626395 | 5.1:+51639727 | None:intergenic |
| ACCACAGTACCAATCACCGC+CGG | 0.655305 | 5.1:-51640246 | MS.gene21305:CDS |
| GCCTCAACAAAGTTTGAGGG+TGG | 0.655336 | 5.1:-51639728 | MS.gene21305:CDS |
| GATGCTGTTTACGGCAACGG+CGG | 0.661829 | 5.1:-51640132 | MS.gene21305:CDS |
| ACCTGATGCTGGTGTGAATG+CGG | 0.662406 | 5.1:-51639752 | MS.gene21305:CDS |
| GCCGGCGGTGATTGGTACTG+TGG | 0.669157 | 5.1:+51640245 | None:intergenic |
| CAAATTCAGTACGAAACGCA+CGG | 0.681403 | 5.1:-51640174 | MS.gene21305:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AGTTGAATTTTTTAGTTATT+TGG | - | chr5.1:51640180-51640199 | MS.gene21305:CDS | 15.0% |
| !!! | GTTGAATTTTTTAGTTATTT+GGG | - | chr5.1:51640181-51640200 | MS.gene21305:CDS | 15.0% |
| !!! | TGTTTATTATTGATTTTTCT+TGG | - | chr5.1:51640064-51640083 | MS.gene21305:CDS | 15.0% |
| !!! | TGTTTTCTAATTGTTGAATA+AGG | - | chr5.1:51640323-51640342 | MS.gene21305:CDS | 20.0% |
| TGGGATTGAAAATTCGTATT+TGG | - | chr5.1:51640200-51640219 | MS.gene21305:CDS | 30.0% | |
| TTTCATGTTACAGGTTCAAT+CGG | - | chr5.1:51640223-51640242 | MS.gene21305:CDS | 30.0% | |
| !! | AAACTTTGAAGTGCAAATTC+AGG | + | chr5.1:51639927-51639946 | None:intergenic | 30.0% |
| AAGGTTCGATTCTCAATTGT+TGG | - | chr5.1:51640017-51640036 | MS.gene21305:intron | 35.0% | |
| ! | AAATTCTCTCTTTGGTGCAA+TGG | - | chr5.1:51639722-51639741 | MS.gene21305:CDS | 35.0% |
| ! | CGTATTTGGTTTCATGTTAC+AGG | - | chr5.1:51640214-51640233 | MS.gene21305:CDS | 35.0% |
| ATTGAGAATCGAACCTTAGC+AGG | + | chr5.1:51640014-51640033 | None:intergenic | 40.0% | |
| ATTGGTACTGTGGTGTTCAA+AGG | + | chr5.1:51639774-51639793 | None:intergenic | 40.0% | |
| CAAATTCAGTACGAAACGCA+CGG | - | chr5.1:51639852-51639871 | MS.gene21305:intron | 40.0% | |
| CGCTTCTCAAATTCTCTCTT+TGG | - | chr5.1:51639714-51639733 | MS.gene21305:CDS | 40.0% | |
| !! | CAATCGGTGTTGTTACTGTT+GGG | - | chr5.1:51640239-51640258 | MS.gene21305:CDS | 40.0% |
| !! | TCAATCGGTGTTGTTACTGT+TGG | - | chr5.1:51640238-51640257 | MS.gene21305:CDS | 40.0% |
| ACCACCCTCAAACTTTGTTG+AGG | + | chr5.1:51640302-51640321 | None:intergenic | 45.0% | |
| GGTGCCTCAACAAAGTTTGA+GGG | - | chr5.1:51640295-51640314 | MS.gene21305:CDS | 45.0% | |
| ! | TCCAACTCCGATGCTGTTTA+CGG | - | chr5.1:51639885-51639904 | MS.gene21305:intron | 45.0% |
| !! | GGTTGAGAGTGTTCGTACAT+TGG | + | chr5.1:51639753-51639772 | None:intergenic | 45.0% |
| !! | TGAGAGTGTTCGTACATTGG+TGG | + | chr5.1:51639750-51639769 | None:intergenic | 45.0% |
| ACCTGATGCTGGTGTGAATG+CGG | - | chr5.1:51640274-51640293 | MS.gene21305:CDS | 50.0% | |
| CGGTGCCTCAACAAAGTTTG+AGG | - | chr5.1:51640294-51640313 | MS.gene21305:CDS | 50.0% | |
| GCCTCAACAAAGTTTGAGGG+TGG | - | chr5.1:51640298-51640317 | MS.gene21305:CDS | 50.0% | |
| GGATGTGAACAACCTGATGC+TGG | - | chr5.1:51640263-51640282 | MS.gene21305:CDS | 50.0% | |
| TCAGTACGAAACGCACGGTA+TGG | - | chr5.1:51639857-51639876 | MS.gene21305:intron | 50.0% | |
| TGACTCTGTTACGCCTGCTA+AGG | - | chr5.1:51639998-51640017 | MS.gene21305:intron | 50.0% | |
| ! | TCCGATGCTGTTTACGGCAA+CGG | - | chr5.1:51639891-51639910 | MS.gene21305:intron | 50.0% |
| !! | TCGGTGTTGTTACTGTTGGG+TGG | - | chr5.1:51640242-51640261 | MS.gene21305:CDS | 50.0% |
| !! | TTGATGTTGAGCGTCGATGC+CGG | + | chr5.1:51639835-51639854 | None:intergenic | 50.0% |
| ACCACAGTACCAATCACCGC+CGG | - | chr5.1:51639780-51639799 | MS.gene21305:CDS | 55.0% | |
| ACCGCATTCACACCAGCATC+AGG | + | chr5.1:51640278-51640297 | None:intergenic | 55.0% | |
| GCCGTTGCCGTAAACAGCAT+CGG | + | chr5.1:51639895-51639914 | None:intergenic | 55.0% | |
| ! | CAGCTTACGCTGTCGTTTCG+TGG | - | chr5.1:51639960-51639979 | MS.gene21305:intron | 55.0% |
| ! | GATGCTGTTTACGGCAACGG+CGG | - | chr5.1:51639894-51639913 | MS.gene21305:intron | 55.0% |
| ! | GCCGTAAACAGCATCGGAGT+TGG | + | chr5.1:51639889-51639908 | None:intergenic | 55.0% |
| GGAACCGTCATCGCTTTCGC+CGG | + | chr5.1:51639802-51639821 | None:intergenic | 60.0% | |
| ! | GGCGAAAGCGATGACGGTTC+CGG | - | chr5.1:51639801-51639820 | MS.gene21305:intron | 60.0% |
| ACCGCCGGCGAAAGCGATGA+CGG | - | chr5.1:51639795-51639814 | MS.gene21305:intron | 65.0% | |
| ACCGTCATCGCTTTCGCCGG+CGG | + | chr5.1:51639799-51639818 | None:intergenic | 65.0% | |
| GCCGGCGGTGATTGGTACTG+TGG | + | chr5.1:51639784-51639803 | None:intergenic | 65.0% | |
| TCGCTTTCGCCGGCGGTGAT+TGG | + | chr5.1:51639792-51639811 | None:intergenic | 65.0% | |
| !! | TTGAGCGTCGATGCCGGCGT+CGG | + | chr5.1:51639829-51639848 | None:intergenic | 65.0% |
| !! | TGAGCGTCGATGCCGGCGTC+GGG | + | chr5.1:51639828-51639847 | None:intergenic | 70.0% |
| GACGGTTCCGGTCCCGACGC+CGG | - | chr5.1:51639813-51639832 | MS.gene21305:intron | 75.0% | |
| ! | GTCGATGCCGGCGTCGGGAC+CGG | + | chr5.1:51639823-51639842 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 51639705 | 51640343 | 51639705 | ID=MS.gene21305 |
| chr5.1 | mRNA | 51639705 | 51640343 | 51639705 | ID=MS.gene21305.t1;Parent=MS.gene21305 |
| chr5.1 | exon | 51640029 | 51640343 | 51640029 | ID=MS.gene21305.t1.exon1;Parent=MS.gene21305.t1 |
| chr5.1 | CDS | 51640029 | 51640343 | 51640029 | ID=cds.MS.gene21305.t1;Parent=MS.gene21305.t1 |
| chr5.1 | exon | 51639705 | 51639812 | 51639705 | ID=MS.gene21305.t1.exon2;Parent=MS.gene21305.t1 |
| chr5.1 | CDS | 51639705 | 51639812 | 51639705 | ID=cds.MS.gene21305.t1;Parent=MS.gene21305.t1 |
| Gene Sequence |
| Protein sequence |
