AlfalfaGEDB | MS.gene21305

M. sativa cultivar XinJiangDaYe / MS.gene21305

KEGG: -
GO: -
PFAM: PF06200.17

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21305.t1	KEH28124.1	95.9	121	5	0	13	133	1	121	3.00E-60	241.1

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21305.t1	Q8H1G0	41.9	105	43	2	47	133	28	132	1.6e-09	63.9

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21305.t1	A0A072UEB1	95.9	121	5	0	13	133	1	121	2.2e-60	241.1

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene21305.t1	TF	Tify

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21305.t1	MTR_5g066510	95.868	121	5	0	13	133	1	121	6.94e-84	244
MS.gene21305.t1	MTR_5g066510	95.868	121	5	0	13	133	1	121	1.22e-82	244
MS.gene21305.t1	MTR_1g061610	67.647	68	21	1	51	118	42	108	1.13e-25	94.4
MS.gene21305.t1	MTR_3g100050	67.213	61	19	1	58	117	42	102	1.52e-19	82.8

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21305.t1	AT3G21175	44.565	92	29	4	50	119	21	112	1.51e-12	62.4
MS.gene21305.t1	AT1G51600	62.963	54	18	1	82	133	79	132	1.62e-12	63.5
MS.gene21305.t1	AT1G51600	62.963	54	18	1	82	133	79	132	1.62e-12	63.5
MS.gene21305.t1	AT3G21175	41.284	109	40	5	50	134	21	129	2.73e-12	62.8
MS.gene21305.t1	AT3G21175	44.565	92	29	4	50	119	21	112	4.22e-12	62.0

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Find 39 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTTTGAAGTGCAAATTC+AGG	0.278085	5.1:+51640102	None:intergenic
AAATTCTCTCTTTGGTGCAA+TGG	0.471067	5.1:-51640304	MS.gene21305:CDS
ACCACAGTACCAATCACCGC+CGG	0.655305	5.1:-51640246	MS.gene21305:CDS
ACCACCCTCAAACTTTGTTG+AGG	0.626395	5.1:+51639727	None:intergenic
ACCGCATTCACACCAGCATC+AGG	0.496179	5.1:+51639751	None:intergenic
ACCGCCGGCGAAAGCGATGA+CGG	0.576304	5.1:-51640231	MS.gene21305:CDS
ACCGTCATCGCTTTCGCCGG+CGG	0.524563	5.1:+51640230	None:intergenic
ACCTGATGCTGGTGTGAATG+CGG	0.662406	5.1:-51639752	MS.gene21305:CDS
ATTGAGAATCGAACCTTAGC+AGG	0.477071	5.1:+51640015	None:intergenic
ATTGGTACTGTGGTGTTCAA+AGG	0.519474	5.1:+51640255	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
TGGGATTGAAAATTCGTATT+TGG	-	chr5.1:51640200-51640219	MS.gene21305:CDS	30.0%
TTTCATGTTACAGGTTCAAT+CGG	-	chr5.1:51640223-51640242	MS.gene21305:CDS	30.0%
AAGGTTCGATTCTCAATTGT+TGG	-	chr5.1:51640017-51640036	MS.gene21305:intron	35.0%
ATTGAGAATCGAACCTTAGC+AGG	+	chr5.1:51640014-51640033	None:intergenic	40.0%
ATTGGTACTGTGGTGTTCAA+AGG	+	chr5.1:51639774-51639793	None:intergenic	40.0%
CAAATTCAGTACGAAACGCA+CGG	-	chr5.1:51639852-51639871	MS.gene21305:intron	40.0%
CGCTTCTCAAATTCTCTCTT+TGG	-	chr5.1:51639714-51639733	MS.gene21305:CDS	40.0%
ACCACCCTCAAACTTTGTTG+AGG	+	chr5.1:51640302-51640321	None:intergenic	45.0%
GGTGCCTCAACAAAGTTTGA+GGG	-	chr5.1:51640295-51640314	MS.gene21305:CDS	45.0%
ACCTGATGCTGGTGTGAATG+CGG	-	chr5.1:51640274-51640293	MS.gene21305:CDS	50.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.1	gene	51639705	51640343	51639705	ID=MS.gene21305
chr5.1	mRNA	51639705	51640343	51639705	ID=MS.gene21305.t1;Parent=MS.gene21305
chr5.1	exon	51640029	51640343	51640029	ID=MS.gene21305.t1.exon1;Parent=MS.gene21305.t1
chr5.1	CDS	51640029	51640343	51640029	ID=cds.MS.gene21305.t1;Parent=MS.gene21305.t1
chr5.1	exon	51639705	51639812	51639705	ID=MS.gene21305.t1.exon2;Parent=MS.gene21305.t1
chr5.1	CDS	51639705	51639812	51639705	ID=cds.MS.gene21305.t1;Parent=MS.gene21305.t1

Gene Sequence

>MS.gene21305

GGTTTCAATCGCTTCTCAAATTCTCTCTTTGGTGCAATGGAACCACCAATGTACGAACACTCTCAACCTTTGAACACCACAGTACCAATCACCGCCGGCGAAAGCGATGACGGTTCCGGTCCCGACGCCGGCATCGACGCTCAACATCAAATTCAGTACGAAACGCACGGTATGGATGATTCCAACTCCGATGCTGTTTACGGCAACGGCGGCGAAAATCCTGAATTTGCACTTCAAAGTTTTGATGAATCTGATCAGCTTACGCTGTCGTTTCGTGGTCAAGTTTATGTCTTTGACTCTGTTACGCCTGCTAAGGTTCGATTCTCAATTGTTGGAATTCTTTTTCTGTGATTTTATTTTGTTTATTATTGATTTTTCTTGGTGATTTTGTGACACTTTCGTTACTGAAATTGATTTAAGCTGAATAATGAATAATGAACGAAATAAATTTGATTTTTTTTTTGTTGTTGTTAAAAGTTGAATTTTTTAGTTATTTGGGATTGAAAATTCGTATTTGGTTTCATGTTACAGGTTCAATCGGTGTTGTTACTGTTGGGTGGATGTGAACAACCTGATGCTGGTGTGAATGCGGTGCCTCAACAAAGTTTGAGGGTGGTATGTTTTCTAATTGTTGAATAA

Protein sequence

>MS.gene21305.t1

GFNRFSNSLFGAMEPPMYEHSQPLNTTVPITAGESDDGSGPDAGIDAQHQIQYETHGMDDSNSDAVYGNGGENPEFALQSFDESDQLTLSFRGQVYVFDSVTPAKVQSVLLLLGGCEQPDAGVNAVPQQSLRVVCFLIVE