AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21333

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21333.t1 MTR_1g089830 74.510 153 15 2 12 152 5 145 5.09e-75 220
MS.gene21333.t1 MTR_1g045040 71.324 136 32 1 10 145 3 131 1.31e-58 178
MS.gene21333.t1 MTR_8g089170 42.857 147 65 5 13 141 7 152 7.48e-29 103
MS.gene21333.t1 MTR_8g089130 40.397 151 71 4 13 144 7 157 5.54e-28 101
MS.gene21333.t1 MTR_8g039350 84.615 39 6 0 15 53 19 57 1.06e-18 75.5
MS.gene21333.t1 MTR_1g075130 66.216 74 21 1 74 147 14 83 8.72e-18 73.6
MS.gene21333.t1 MTR_4g028990 43.956 91 48 2 56 144 7 96 6.34e-17 71.6
MS.gene21333.t1 MTR_5g013370 50.820 61 29 1 3 63 5 64 1.76e-15 72.8
MS.gene21333.t1 MTR_5g013370 50.820 61 29 1 3 63 5 64 2.56e-15 72.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21333.t1 AT4G17570 60.465 43 17 0 3 45 5 47 1.58e-14 70.1
MS.gene21333.t1 AT4G17570 60.465 43 17 0 3 45 5 47 1.63e-14 70.1
MS.gene21333.t1 AT5G47140 58.140 43 18 0 3 45 5 47 4.60e-14 68.6

Find 30 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGTTGGGTTTGAAAGCTT+TGG 0.278312 1.4:+71176009 None:intergenic
GAGTTGGGTTTGAAAGCTTT+GGG 0.313526 1.4:+71176010 None:intergenic
ATAGTCTTCAAGGGTTCCTT+TGG 0.332237 1.4:+71176098 None:intergenic
TCAAACAACAAAATATTATC+TGG 0.350768 1.4:+71174177 None:intergenic
CACAAGATTGGCTTCTCATA+TGG 0.363409 1.4:+71176147 None:intergenic
TTTCAACAGAAACTAAAGTT+TGG 0.373286 1.4:-71176179 MS.gene21333:intron
ATACTTTGCTGAGCTATCTT+CGG 0.400089 1.4:+71176072 None:intergenic
GACTATGAGGCCGACACTAA+TGG 0.401191 1.4:-71174280 MS.gene21333:CDS
TACTTTGCTGAGCTATCTTC+GGG 0.431538 1.4:+71176073 None:intergenic
CCAAGTAATGAGATCGGCCT+TGG 0.442800 1.4:-71174135 MS.gene21333:CDS
CCACAGGCGTTACACAAGAT+TGG 0.446240 1.4:+71176135 None:intergenic
TGTGTGCATTGTGGAATTAA+TGG 0.447647 1.4:-71176819 MS.gene21333:intron
GAAACTAAAGTTTGGCGTAA+AGG 0.450000 1.4:-71176171 MS.gene21333:CDS
TCTTCGGGAGATAGTCTTCA+AGG 0.462167 1.4:+71176088 None:intergenic
CCAAGGCCGATCTCATTACT+TGG 0.469229 1.4:+71174135 None:intergenic
CGCCTGTGGATTGCATTTCA+AGG 0.472275 1.4:-71176121 MS.gene21333:CDS
GGCCTTGAAATGCAATCCAC+AGG 0.512475 1.4:+71176119 None:intergenic
AATGGAGACGCTGACACCAA+TGG 0.564023 1.4:-71174262 MS.gene21333:CDS
AAGCTTTCAAACCCAACTCC+AGG 0.571736 1.4:-71176006 MS.gene21333:intron
ATTAGTGTCGGCCTCATAGT+CGG 0.574226 1.4:+71174282 None:intergenic
GTCAGCGTCTCCATTAGTGT+CGG 0.583147 1.4:+71174270 None:intergenic
AGAGGTCGGCACCGACTATG+AGG 0.586753 1.4:-71174293 MS.gene21333:CDS
TTAATTCCACAATGCACACA+TGG 0.587961 1.4:+71176822 None:intergenic
TTTATACCAAGTAATGAGAT+CGG 0.595926 1.4:-71174141 MS.gene21333:CDS
CCAATCTTGTGTAACGCCTG+TGG 0.600058 1.4:-71176135 MS.gene21333:CDS
CTTCGGGAGATAGTCTTCAA+GGG 0.601274 1.4:+71176089 None:intergenic
TCTTTAGAAGAGTGCAGCCA+AGG 0.606342 1.4:+71174118 None:intergenic
GGATTGCATTTCAAGGCCAA+AGG 0.606742 1.4:-71176114 MS.gene21333:CDS
GCGGGTCCATGTGTGCATTG+TGG 0.639689 1.4:-71176828 MS.gene21333:CDS
GGAGACGCTGACACCAATGG+AGG 0.714256 1.4:-71174259 MS.gene21333:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTCTTAAAATTGTAATT+TGG - chr1.4:71174585-71174604 MS.gene21333:intron 10.0%
!! AATAAAATTAACAATAAGGA+AGG + chr1.4:71174495-71174514 None:intergenic 15.0%
!! AGTATTGTTAAAAAAAAAAG+AGG + chr1.4:71174409-71174428 None:intergenic 15.0%
!! ATAAATTTATTGGATGTATT+TGG - chr1.4:71174244-71174263 MS.gene21333:CDS 15.0%
!! ATAGAATCATCTTTAAATTA+TGG + chr1.4:71175684-71175703 None:intergenic 15.0%
!! ATATTGAACTAATCTAATAT+CGG - chr1.4:71174374-71174393 MS.gene21333:intron 15.0%
!! TAATCATTTATTTGTATCAT+CGG + chr1.4:71176531-71176550 None:intergenic 15.0%
!! TCATATACAATACATATTAA+TGG + chr1.4:71176555-71176574 None:intergenic 15.0%
!! TTTAAGCTAATGATATTATA+AGG - chr1.4:71175893-71175912 MS.gene21333:intron 15.0%
!!! ATATATTTTAAAAACTTCAG+CGG + chr1.4:71175065-71175084 None:intergenic 15.0%
!!! TAAAAATAAAAGTGTATATG+CGG + chr1.4:71174637-71174656 None:intergenic 15.0%
!!! TTATAATATCATTAGCTTAA+AGG + chr1.4:71175894-71175913 None:intergenic 15.0%
!! ACAACTCATATATAAATGTT+AGG + chr1.4:71175238-71175257 None:intergenic 20.0%
!! ATCAATCAATGCATTATAAA+AGG + chr1.4:71175013-71175032 None:intergenic 20.0%
!! ATGCATTATAAAAGGAAAAA+TGG + chr1.4:71175005-71175024 None:intergenic 20.0%
!! CAACTCATATATAAATGTTA+GGG + chr1.4:71175237-71175256 None:intergenic 20.0%
!! CAATAAATTTATGTTCTGTT+CGG + chr1.4:71174236-71174255 None:intergenic 20.0%
!! GAAAAATGGATGATATAAAA+TGG + chr1.4:71174991-71175010 None:intergenic 20.0%
!! TCAAACAACAAAATATTATC+TGG + chr1.4:71176734-71176753 None:intergenic 20.0%
!!! AACAAAAACTTTTCAATCTA+TGG - chr1.4:71176678-71176697 MS.gene21333:intron 20.0%
!!! AATAACTTCAGCTTTAAATT+TGG + chr1.4:71174958-71174977 None:intergenic 20.0%
!!! ACAAAAACTTTTCAATCTAT+GGG - chr1.4:71176679-71176698 MS.gene21333:intron 20.0%
!!! ATTGATAGTGAAGTAAATTA+CGG + chr1.4:71175272-71175291 None:intergenic 20.0%
!!! CTTTAAATTTGGTGAAATAT+GGG + chr1.4:71174947-71174966 None:intergenic 20.0%
!!! TAAAGACACAGTAAAATTTT+AGG + chr1.4:71174545-71174564 None:intergenic 20.0%
!!! TTTACCGTTTTATTTTAGAA+CGG + chr1.4:71174195-71174214 None:intergenic 20.0%
! AAATGGATGATATAAAATGG+AGG + chr1.4:71174988-71175007 None:intergenic 25.0%
! AACATCAAAGAAATTACGAA+TGG + chr1.4:71176021-71176040 None:intergenic 25.0%
! AATGGATGATATAAAATGGA+GGG + chr1.4:71174987-71175006 None:intergenic 25.0%
! ATAGTTTACATTTCCTTGTT+AGG - chr1.4:71176231-71176250 MS.gene21333:intron 25.0%
! ATTGTACGAATATTCTTAAC+GGG - chr1.4:71175181-71175200 MS.gene21333:intron 25.0%
! CATTGTACGAATATTCTTAA+CGG - chr1.4:71175180-71175199 MS.gene21333:intron 25.0%
! CGAACAGAACATAAATTTAT+TGG - chr1.4:71174234-71174253 MS.gene21333:CDS 25.0%
! CGATTGAAAATGAAAATGAT+GGG - chr1.4:71175099-71175118 MS.gene21333:intron 25.0%
! GGAAATCATATTCAAAGTAA+AGG - chr1.4:71174155-71174174 MS.gene21333:CDS 25.0%
! TAATATTTGTGTATGCGATT+CGG + chr1.4:71174332-71174351 None:intergenic 25.0%
! TATTTGTCTAACTTGAAACA+TGG + chr1.4:71175761-71175780 None:intergenic 25.0%
! TCGATTGAAAATGAAAATGA+TGG - chr1.4:71175098-71175117 MS.gene21333:intron 25.0%
! TCGTAATTTCTTTGATGTTA+TGG - chr1.4:71176022-71176041 MS.gene21333:CDS 25.0%
! TTACTTCACTATCAATAAGA+TGG - chr1.4:71175276-71175295 MS.gene21333:intron 25.0%
! TTTATACCAAGTAATGAGAT+CGG - chr1.4:71176767-71176786 MS.gene21333:intron 25.0%
! TTTATTGGATGTATTTGGAT+AGG - chr1.4:71174249-71174268 MS.gene21333:CDS 25.0%
! TTTCAACAGAAACTAAAGTT+TGG - chr1.4:71174729-71174748 MS.gene21333:intron 25.0%
!! AATTTTTATTACCTGGAGTT+GGG + chr1.4:71174916-71174935 None:intergenic 25.0%
!! GAATTGCAATTTTTATTACC+TGG + chr1.4:71174923-71174942 None:intergenic 25.0%
!! GCTTTAAATTTGGTGAAATA+TGG + chr1.4:71174948-71174967 None:intergenic 25.0%
!!! CAGATATTTTTTCAATGTCA+AGG + chr1.4:71174871-71174890 None:intergenic 25.0%
!!! GAAACCGTTCTAAAATAAAA+CGG - chr1.4:71174188-71174207 MS.gene21333:CDS 25.0%
!!! TTGTTCAAAAGTACTATTGT+AGG - chr1.4:71175503-71175522 MS.gene21333:intron 25.0%
AAACAATGAACCATGATGTT+TGG + chr1.4:71175356-71175375 None:intergenic 30.0%
AAAGACAACTAAAGAAGAGT+TGG - chr1.4:71176447-71176466 MS.gene21333:intron 30.0%
AACTTTGTAACCAAACATCA+TGG - chr1.4:71175343-71175362 MS.gene21333:intron 30.0%
AGGAATTATGAAGGTTAGAA+AGG - chr1.4:71175430-71175449 MS.gene21333:intron 30.0%
ATAAGTAGTGTAAAATGGAC+AGG - chr1.4:71175130-71175149 MS.gene21333:intron 30.0%
ATGCCAACATGAGTATTATT+GGG - chr1.4:71176108-71176127 MS.gene21333:CDS 30.0%
CAAATTTCAACTACCTAACA+AGG + chr1.4:71176247-71176266 None:intergenic 30.0%
CATTCACATTCTATTTGAGT+AGG + chr1.4:71175956-71175975 None:intergenic 30.0%
CTTGTTAGGTAGTTGAAATT+TGG - chr1.4:71176245-71176264 MS.gene21333:intron 30.0%
GACTCATAAGTAGTGTAAAA+TGG - chr1.4:71175125-71175144 MS.gene21333:intron 30.0%
GATATTATAAGGTGTGAGAA+TGG - chr1.4:71175904-71175923 MS.gene21333:intron 30.0%
GGAATTATGAAGGTTAGAAA+GGG - chr1.4:71175431-71175450 MS.gene21333:intron 30.0%
TAATACTGATGAGATCAAAC+AGG - chr1.4:71175459-71175478 MS.gene21333:intron 30.0%
TGATGTTTGGTTACAAAGTT+TGG + chr1.4:71175343-71175362 None:intergenic 30.0%
TTCTATCATAAAGTCACCAT+TGG + chr1.4:71175995-71176014 None:intergenic 30.0%
TTTGTAACAACCTCTTTGAT+CGG - chr1.4:71174274-71174293 MS.gene21333:CDS 30.0%
! CAAATGAATCCTCTTTTCAT+TGG + chr1.4:71176325-71176344 None:intergenic 30.0%
! CAATTTTTATTACCTGGAGT+TGG + chr1.4:71174917-71174936 None:intergenic 30.0%
! TCTTTGCTATGTGGATTAAA+GGG - chr1.4:71175712-71175731 MS.gene21333:intron 30.0%
!!! ATGTTCTGTTCGGTTTTTTT+TGG + chr1.4:71174226-71174245 None:intergenic 30.0%
!!! GTTTAGTTTGGATCGATTTT+TGG + chr1.4:71174615-71174634 None:intergenic 30.0%
!!! TAGTACCTTATTTTGTAGAG+AGG - chr1.4:71176597-71176616 MS.gene21333:intron 30.0%
!!! TGTTCTGTTCGGTTTTTTTT+GGG + chr1.4:71174225-71174244 None:intergenic 30.0%
!!! TTTTTTTCCATAGATTCGTG+AGG - chr1.4:71174134-71174153 MS.gene21333:CDS 30.0%
AATATCTGATCCGATCAAAG+AGG + chr1.4:71174287-71174306 None:intergenic 35.0%
AATGCCATTCCAATGAAAAG+AGG - chr1.4:71176313-71176332 MS.gene21333:intron 35.0%
AGGATGTGAATATTGTGAGA+TGG + chr1.4:71175936-71175955 None:intergenic 35.0%
AGTGTATATGCGGTTTAGTT+TGG + chr1.4:71174627-71174646 None:intergenic 35.0%
AGTTTAAGCATGGATTTCCA+TGG - chr1.4:71175591-71175610 MS.gene21333:intron 35.0%
ATAACCTTCTATGACTGCAT+TGG - chr1.4:71175778-71175797 MS.gene21333:intron 35.0%
ATACTTTGCTGAGCTATCTT+CGG + chr1.4:71174839-71174858 None:intergenic 35.0%
ATGATTTCCTCACGAATCTA+TGG + chr1.4:71174144-71174163 None:intergenic 35.0%
CATAGCATCAAAGAAGTCAT+TGG + chr1.4:71176183-71176202 None:intergenic 35.0%
GAAACTAAAGTTTGGCGTAA+AGG - chr1.4:71174737-71174756 MS.gene21333:intron 35.0%
GATAACTTCCAAACAAGTGT+GGG + chr1.4:71176154-71176173 None:intergenic 35.0%
GATGCCAACATGAGTATTAT+TGG - chr1.4:71176107-71176126 MS.gene21333:CDS 35.0%
GTTTAAGCATGGATTTCCAT+GGG - chr1.4:71175592-71175611 MS.gene21333:intron 35.0%
TCTATACCCATCATACCTAT+AGG + chr1.4:71175411-71175430 None:intergenic 35.0%
TGATAACTTCCAAACAAGTG+TGG + chr1.4:71176155-71176174 None:intergenic 35.0%
TGCCAACATGAGTATTATTG+GGG - chr1.4:71176109-71176128 MS.gene21333:CDS 35.0%
TGGGTATAGAGGAATTATGA+AGG - chr1.4:71175421-71175440 MS.gene21333:intron 35.0%
TGTGTGCATTGTGGAATTAA+TGG - chr1.4:71174089-71174108 MS.gene21333:CDS 35.0%
TTAATTCCACAATGCACACA+TGG + chr1.4:71174089-71174108 None:intergenic 35.0%
TTCCCCAATAATACTCATGT+TGG + chr1.4:71176114-71176133 None:intergenic 35.0%
! AATCAGTGAGTTTTTCCTTG+AGG - chr1.4:71176070-71176089 MS.gene21333:CDS 35.0%
! ATTCTTAACGGGTACAATTC+AGG - chr1.4:71175192-71175211 MS.gene21333:intron 35.0%
! GAATCCTCTTTTCATTGGAA+TGG + chr1.4:71176320-71176339 None:intergenic 35.0%
! GTCTTTGCTATGTGGATTAA+AGG - chr1.4:71175711-71175730 MS.gene21333:intron 35.0%
! TTCTTAACGGGTACAATTCA+GGG - chr1.4:71175193-71175212 MS.gene21333:intron 35.0%
! TTTCATTGGAATGGCATTGA+TGG + chr1.4:71176311-71176330 None:intergenic 35.0%
! TTTTGTTGAAACTCCTCCAT+TGG + chr1.4:71176665-71176684 None:intergenic 35.0%
!! AATGGCATTGATGGTTTGAT+AGG + chr1.4:71176302-71176321 None:intergenic 35.0%
!! TATAGGTATGATGGGTATAG+AGG - chr1.4:71175410-71175429 MS.gene21333:intron 35.0%
!!! ACCTTATTTTGTAGAGAGGT+CGG - chr1.4:71176601-71176620 MS.gene21333:intron 35.0%
!!! ATTGGGGAACACCTTTTTTA+TGG - chr1.4:71176125-71176144 MS.gene21333:CDS 35.0%
!!! GTTCTGTTCGGTTTTTTTTG+GGG + chr1.4:71174224-71174243 None:intergenic 35.0%
!!! TTCTGTTCGGTTTTTTTTGG+GGG + chr1.4:71174223-71174242 None:intergenic 35.0%
AACTCCATAGTCTTCATGTC+TGG - chr1.4:71176406-71176425 MS.gene21333:intron 40.0%
ACAACCAATGCAGTCATAGA+AGG + chr1.4:71175785-71175804 None:intergenic 40.0%
ACCATATCAACGTCTTCCAA+TGG - chr1.4:71175976-71175995 MS.gene21333:intron 40.0%
ACCATTGGAAGACGTTGATA+TGG + chr1.4:71175980-71175999 None:intergenic 40.0%
AGTGTGGGACACCATAAAAA+AGG + chr1.4:71176139-71176158 None:intergenic 40.0%
ATAGTCTTCAAGGGTTCCTT+TGG + chr1.4:71174813-71174832 None:intergenic 40.0%
ATCCATGATGCAATCCATTG+TGG - chr1.4:71175555-71175574 MS.gene21333:intron 40.0%
ATCTCCAGACATGAAGACTA+TGG + chr1.4:71176413-71176432 None:intergenic 40.0%
ATGATGCAATCCATTGTGGT+TGG - chr1.4:71175559-71175578 MS.gene21333:intron 40.0%
CAGGTACTCAACATGACAAT+GGG - chr1.4:71175381-71175400 MS.gene21333:intron 40.0%
CATTGTCATGTTGAGTACCT+GGG + chr1.4:71175382-71175401 None:intergenic 40.0%
GAGTTGGGTTTGAAAGCTTT+GGG + chr1.4:71174901-71174920 None:intergenic 40.0%
GGTTCTCCTATAGGTATGAT+GGG - chr1.4:71175402-71175421 MS.gene21333:intron 40.0%
TACTTTGCTGAGCTATCTTC+GGG + chr1.4:71174838-71174857 None:intergenic 40.0%
TGATGCAATCCATTGTGGTT+GGG - chr1.4:71175560-71175579 MS.gene21333:intron 40.0%
! ACGCTGCTAAAGTTTTCTAC+GGG + chr1.4:71175648-71175667 None:intergenic 40.0%
! ATGACAATGGGTTCTCCTAT+AGG - chr1.4:71175393-71175412 MS.gene21333:intron 40.0%
! CACGCTGCTAAAGTTTTCTA+CGG + chr1.4:71175649-71175668 None:intergenic 40.0%
! TCTTAACGGGTACAATTCAG+GGG - chr1.4:71175194-71175213 MS.gene21333:intron 40.0%
! TGAGAGTTGTCTTTGCTATG+TGG - chr1.4:71175703-71175722 MS.gene21333:intron 40.0%
!! AAAGTTTTCTACGGGTTGCA+TGG + chr1.4:71175640-71175659 None:intergenic 40.0%
!! AACCACAATGGATTGCATCA+TGG + chr1.4:71175560-71175579 None:intergenic 40.0%
!! CACAAGATTGGCTTCTCATA+TGG + chr1.4:71174764-71174783 None:intergenic 40.0%
!! TTCTATGACTGCATTGGTTG+TGG - chr1.4:71175784-71175803 MS.gene21333:intron 40.0%
!!! TTCGGTTTTTTTTGGGGGAT+TGG + chr1.4:71174218-71174237 None:intergenic 40.0%
AAGCTTTCAAACCCAACTCC+AGG - chr1.4:71174902-71174921 MS.gene21333:intron 45.0%
ATGGTTCATTGTTTGTGCCC+AGG - chr1.4:71175362-71175381 MS.gene21333:intron 45.0%
CCAGGTACTCAACATGACAA+TGG - chr1.4:71175380-71175399 MS.gene21333:intron 45.0%
CCATTGTCATGTTGAGTACC+TGG + chr1.4:71175383-71175402 None:intergenic 45.0%
CTTCGGGAGATAGTCTTCAA+GGG + chr1.4:71174822-71174841 None:intergenic 45.0%
GCCGACCTCTCTACAAAATA+AGG + chr1.4:71176605-71176624 None:intergenic 45.0%
GGAGTTGGGTTTGAAAGCTT+TGG + chr1.4:71174902-71174921 None:intergenic 45.0%
GGATTGCATTTCAAGGCCAA+AGG - chr1.4:71174794-71174813 MS.gene21333:intron 45.0%
GGCATGGTGTAGTTTAAGCA+TGG - chr1.4:71175581-71175600 MS.gene21333:intron 45.0%
GGGTTCTCCTATAGGTATGA+TGG - chr1.4:71175401-71175420 MS.gene21333:intron 45.0%
GGTTACCCAGAATGTCCAAA+CGG + chr1.4:71175619-71175638 None:intergenic 45.0%
TATGGTGTCCCACACTTGTT+TGG - chr1.4:71176143-71176162 MS.gene21333:CDS 45.0%
TCTTCGGGAGATAGTCTTCA+AGG + chr1.4:71174823-71174842 None:intergenic 45.0%
! GTTTTTCCTTGAGGTCCTTG+AGG - chr1.4:71176079-71176098 MS.gene21333:CDS 45.0%
! TCTTTAGAAGAGTGCAGCCA+AGG + chr1.4:71176793-71176812 None:intergenic 45.0%
!! ATTAGTGTCGGCCTCATAGT+CGG + chr1.4:71176629-71176648 None:intergenic 45.0%
!! GGCATTGATGGTTTGATAGG+TGG + chr1.4:71176299-71176318 None:intergenic 45.0%
!! ATATAATAAAATTAACAATA+AGG + chr1.4:71174499-71174518 None:intergenic 5.0%
AATGGAGACGCTGACACCAA+TGG - chr1.4:71176646-71176665 MS.gene21333:intron 50.0%
AGAATGTCCAAACGGACCCA+TGG + chr1.4:71175611-71175630 None:intergenic 50.0%
ATGGGTCCGTTTGGACATTC+TGG - chr1.4:71175610-71175629 MS.gene21333:intron 50.0%
CAATCCATTGTGGTTGGGCA+TGG - chr1.4:71175565-71175584 MS.gene21333:intron 50.0%
CCAAGGCCGATCTCATTACT+TGG + chr1.4:71176776-71176795 None:intergenic 50.0%
CCAAGTAATGAGATCGGCCT+TGG - chr1.4:71176773-71176792 MS.gene21333:intron 50.0%
CCAATCTTGTGTAACGCCTG+TGG - chr1.4:71174773-71174792 MS.gene21333:intron 50.0%
CCACAGGCGTTACACAAGAT+TGG + chr1.4:71174776-71174795 None:intergenic 50.0%
GACTATGAGGCCGACACTAA+TGG - chr1.4:71176628-71176647 MS.gene21333:intron 50.0%
GGCCTTGAAATGCAATCCAC+AGG + chr1.4:71174792-71174811 None:intergenic 50.0%
GTCAGCGTCTCCATTAGTGT+CGG + chr1.4:71176641-71176660 None:intergenic 50.0%
TACACCATGCCCAACCACAA+TGG + chr1.4:71175572-71175591 None:intergenic 50.0%
TGGATTTCCATGGGTCCGTT+TGG - chr1.4:71175601-71175620 MS.gene21333:intron 50.0%
TGGGTCCGTTTGGACATTCT+GGG - chr1.4:71175611-71175630 MS.gene21333:intron 50.0%
TGTTGGCATCATGCACCTCA+AGG + chr1.4:71176097-71176116 None:intergenic 50.0%
! CGCCTGTGGATTGCATTTCA+AGG - chr1.4:71174787-71174806 MS.gene21333:intron 50.0%
CATGCACCTCAAGGACCTCA+AGG + chr1.4:71176088-71176107 None:intergenic 55.0%
GCGGGTCCATGTGTGCATTG+TGG - chr1.4:71174080-71174099 MS.gene21333:CDS 60.0%
GGAGACGCTGACACCAATGG+AGG - chr1.4:71176649-71176668 MS.gene21333:intron 60.0%
!! AGAGGTCGGCACCGACTATG+AGG - chr1.4:71176615-71176634 MS.gene21333:intron 60.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 71174077 71176853 71174077 ID=MS.gene21333
chr1.4 mRNA 71174077 71176853 71174077 ID=MS.gene21333.t1;Parent=MS.gene21333
chr1.4 exon 71176820 71176853 71176820 ID=MS.gene21333.t1.exon1;Parent=MS.gene21333.t1
chr1.4 CDS 71176820 71176853 71176820 ID=cds.MS.gene21333.t1;Parent=MS.gene21333.t1
chr1.4 exon 71176007 71176192 71176007 ID=MS.gene21333.t1.exon2;Parent=MS.gene21333.t1
chr1.4 CDS 71176007 71176192 71176007 ID=cds.MS.gene21333.t1;Parent=MS.gene21333.t1
chr1.4 exon 71174077 71174315 71174077 ID=MS.gene21333.t1.exon3;Parent=MS.gene21333.t1
chr1.4 CDS 71174077 71174315 71174077 ID=cds.MS.gene21333.t1;Parent=MS.gene21333.t1
Gene Sequence

>MS.gene21333

ATGGCGGGTCCATGTGTGCATTGTGGAATTAATGGTAAGTTATCTCATATCTTGAGTTTTTTTTCCATAGATTCGTGAGGAAATCATATTCAAAGTAAAGGTGTTTACATCGAAACCGTTCTAAAATAAAACGGTAAACCAATCCCCCAAAAAAAACCGAACAGAACATAAATTTATTGGATGTATTTGGATAGGCTTTTGTAACAACCTCTTTGATCGGATCAGATATTGAATTTCATTTTCAAAATCAAACCGAATCGCATACACAAATATTAAAAAAAAAATTATATGTATACAATATTGAACTAATCTAATATCGGCTTGTTTTTCCTCTTTTTTTTTTAACAATACTTATTATTTAAATTTAATTCAATTTTTTGACTATTTCATGTGTCGCAAACGTACTCGATTTTTTCCTTCCTTATTGTTAATTTTATTATATTATATGTTATATTTTGCATCAAACCTAAAATTTTACTGTGTCTTTATAATATTAATTTTAATAGATTTTTTCTTAAAATTGTAATTTGGATATCCAAAAATCGATCCAAACTAAACCGCATATACACTTTTATTTTTATTTTACTCTAGTTATTAACGCGAATCTCATCTTACATTATTATCACTCTTGTTTATTTATATGTTAACATGATTTCAACAGAAACTAAAGTTTGGCGTAAAGGACCATATGAGAAGCCAATCTTGTGTAACGCCTGTGGATTGCATTTCAAGGCCAAAGGAACCCTTGAAGACTATCTCCCGAAGATAGCTCAGCAAAGTATGTCATCAGACCTTGACATTGAAAAAATATCTGAAAGTGACCCAAAGCTTTCAAACCCAACTCCAGGTAATAAAAATTGCAATTCACCCATATTTCACCAAATTTAAAGCTGAAGTTATTCAATTTCCCTCCATTTTATATCATCCATTTTTCCTTTTATAATGCATTGATTGATTTTATTGTAATGTGTGAGAAAGTCACACGCCGCTGAAGTTTTTAAAATATATATACTCAAATTAATCGATTGAAAATGAAAATGATGGGCATGACTCATAAGTAGTGTAAAATGGACAGGACAAGCAAGAAGAGTGATCAAGAAATACATTGTACGAATATTCTTAACGGGTACAATTCAGGGGTATTGTTATAATTTGAACCCTAACATTTATATATGAGTTGTAACATTGTAAACCGTAATTTACTTCACTATCAATAAGATGGAACTACAATTGATCTATAGTCAAAGACGTAGACATACTTTGCCAAACTTTGTAACCAAACATCATGGTTCATTGTTTGTGCCCAGGTACTCAACATGACAATGGGTTCTCCTATAGGTATGATGGGTATAGAGGAATTATGAAGGTTAGAAAGGGTGTCTTAATACTGATGAGATCAAACAGGATTGCATGTAACGTCTACATATTGTTCAAAAGTACTATTGTAGGTGATGTTGCGCAAATCTAGTTTGACAACGATCCATGATGCAATCCATTGTGGTTGGGCATGGTGTAGTTTAAGCATGGATTTCCATGGGTCCGTTTGGACATTCTGGGTAACCATGCAACCCGTAGAAAACTTTAGCAGCGTGTTTTGATTTCTTCCATAATTTAAAGATGATTCTATGAGAGTTGTCTTTGCTATGTGGATTAAAGGGTGTTTGAGATATCATATGAGCTTCCATGTTTCAAGTTAGACAAATAACCTTCTATGACTGCATTGGTTGTGGAATTGAATGATACAAATGTGTTATCATCGATGATTTTTTCTCTTTTTAGATAGTTCTTAAAATTAGATAAAATATCATGAAGATCCTTTAAGCTAATGATATTATAAGGTGTGAGAATGGATGCAGCCATCTCACAATATTCACATCCTACTCAAATAGAATGTGAATGACCATATCAACGTCTTCCAATGGTGACTTTATGATAGAAATACCATTCGTAATTTCTTTGATGTTATGGAAATGTTCAAAAATTAGAGTTGAAGAATCAGTGAGTTTTTCCTTGAGGTCCTTGAGGTGCATGATGCCAACATGAGTATTATTGGGGAACACCTTTTTTATGGTGTCCCACACTTGTTTGGAAGTTATCAAATTACCAATGACTTCTTTGATGCTATGATATACATAAATGATAATAAATACAAGAATAGTTTACATTTCCTTGTTAGGTAGTTGAAATTTGGATGATCTGCAGCAAGACATAGCTTCTTGCCACCTATCAAACCATCAATGCCATTCCAATGAAAAGAGGATTCATTTGAGTAGACACAATGCTAGATTTTGAATTAATTGTATTTTTGTGCATTAGATAACACACATACAACTCCATAGTCTTCATGTCTGGAGATAATATTGCTAATAAAAAGACAACTAAAGAAGAGTTGGTCTAAGATTGATCAATCAATCTCATTCTTATATTTACACATTAATAGCTAATATTCTTCCGATGATACAAATAAATGATTATCCATTAATATGTATTGTATATGATGTATAATATTTTTTTAATCATTAGTACCTTATTTTGTAGAGAGGTCGGCACCGACTATGAGGCCGACACTAATGGAGACGCTGACACCAATGGAGGAGTTTCAACAAAAACTTTTCAATCTATGGGAATCTGAGAAAGAAGCAAATGAATCATCTCCAGATAATATTTTGTTGTTTGATAATATGAACAACTTTATACCAAGTAATGAGATCGGCCTTGGCTGCACTCTTCTAAAGACAGATGTTACTTCTAAAGATCAACATGATTCACATGCATAG
Protein sequence

>MS.gene21333.t1

MAGPCVHCGINETKVWRKGPYEKPILCNACGLHFKAKGTLEDYLPKIAQQSMSSDLDIEKISESDPKLSNPTPERSAPTMRPTLMETLTPMEEFQQKLFNLWESEKEANESSPDNILLFDNMNNFIPSNEIGLGCTLLKTDVTSKDQHDSHA