AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21622

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21622.t1 MTR_4g102720 71.345 171 16 1 1 171 1 138 1.01e-77 248
MS.gene21622.t1 MTR_5g039760 38.000 200 98 3 4 201 7 182 2.01e-37 138
MS.gene21622.t1 MTR_7g074790 43.023 172 79 2 3 173 6 159 1.16e-36 137
MS.gene21622.t1 MTR_0402s0040 42.614 176 97 3 7 179 26 200 6.04e-36 142
MS.gene21622.t1 MTR_1g083640 29.536 237 143 5 2 217 49 282 2.97e-29 118
MS.gene21622.t1 MTR_0558s0020 28.692 237 145 5 2 217 34 267 2.81e-28 115
MS.gene21622.t1 MTR_7g061980 46.018 113 60 1 4 115 7 119 3.87e-28 109
MS.gene21622.t1 MTR_5g015480 45.455 132 68 3 7 135 56 186 2.08e-26 109
MS.gene21622.t1 MTR_6g051425 30.357 224 141 7 6 217 13 233 6.83e-26 109
MS.gene21622.t1 MTR_6g090505 26.910 301 186 8 52 333 21 306 2.16e-25 108
MS.gene21622.t1 MTR_4g035820 28.312 385 213 15 7 367 75 420 4.16e-23 105
MS.gene21622.t1 MTR_8g446020 29.358 218 129 5 2 217 33 227 3.86e-22 97.1
MS.gene21622.t1 MTR_2g070590 45.631 103 53 2 7 106 17 119 1.02e-21 91.7
MS.gene21622.t1 MTR_6g017200 46.875 96 48 2 7 99 58 153 4.69e-20 87.8
MS.gene21622.t1 MTR_0337s0040 30.519 154 105 1 62 213 71 224 1.77e-18 85.5
MS.gene21622.t1 MTR_2g014100 28.019 207 125 6 2 187 42 245 2.07e-18 85.9
MS.gene21622.t1 MTR_1g057920 48.148 81 41 1 7 86 34 114 3.56e-18 81.6
MS.gene21622.t1 MTR_2g099430 49.425 87 43 1 30 115 26 112 7.24e-18 80.5
MS.gene21622.t1 MTR_1g070355 27.488 211 143 4 13 217 80 286 9.73e-18 87.8
MS.gene21622.t1 MTR_7g446520 27.404 208 137 1 10 217 91 284 3.84e-17 83.6
MS.gene21622.t1 MTR_1g013060 27.700 213 146 4 2 207 19 230 2.55e-16 79.3
MS.gene21622.t1 MTR_4g067290 30.539 167 106 6 6 164 55 219 6.56e-16 78.2
MS.gene21622.t1 MTR_4g067290 32.639 144 86 5 6 143 75 213 1.57e-15 77.4
MS.gene21622.t1 MTR_4g067290 32.639 144 86 5 6 143 115 253 2.01e-15 78.2
MS.gene21622.t1 MTR_7g113010 38.889 126 62 2 7 130 457 569 3.56e-15 79.7
MS.gene21622.t1 MTR_2g098920 37.864 103 61 3 6 106 79 180 5.03e-15 76.3
MS.gene21622.t1 MTR_4g067290 38.835 103 60 3 6 106 55 156 5.84e-15 75.5
MS.gene21622.t1 MTR_5g076070 26.168 214 142 5 13 217 59 265 6.64e-15 76.3
MS.gene21622.t1 MTR_4g067290 38.835 103 60 3 6 106 73 174 1.20e-14 74.7
MS.gene21622.t1 MTR_4g067290 38.835 103 60 3 6 106 75 176 1.26e-14 74.7
MS.gene21622.t1 MTR_1g008290 30.935 139 93 2 64 202 9 144 6.61e-14 70.1
MS.gene21622.t1 MTR_0337s0060 29.189 185 95 5 284 461 20 175 6.67e-14 72.4
MS.gene21622.t1 MTR_7g446510 30.709 127 88 0 91 217 166 292 5.55e-12 68.2
MS.gene21622.t1 MTR_0137s0040 51.786 56 26 1 4 58 33 88 6.10e-12 63.2
MS.gene21622.t1 MTR_1g055240 31.452 124 83 1 62 183 21 144 1.13e-11 63.9
MS.gene21622.t1 MTR_7g446480 30.709 127 88 0 91 217 151 277 1.58e-11 66.6
MS.gene21622.t1 MTR_6g022080 28.767 146 100 2 19 160 97 242 1.63e-11 65.5
MS.gene21622.t1 MTR_4g040430 27.485 171 100 6 2 153 33 198 4.36e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21622.t1 AT4G38180 37.615 218 130 4 4 216 70 286 2.66e-34 140
MS.gene21622.t1 AT4G38180 32.068 237 154 3 222 457 408 638 2.68e-31 131
MS.gene21622.t1 AT3G22170 29.487 312 192 7 222 520 393 689 2.70e-33 137
MS.gene21622.t1 AT3G22170 25.551 227 131 6 6 217 69 272 2.28e-13 74.3
MS.gene21622.t1 AT3G22170 29.487 312 192 7 222 520 393 689 2.70e-33 137
MS.gene21622.t1 AT3G22170 25.551 227 131 6 6 217 69 272 2.28e-13 74.3
MS.gene21622.t1 AT4G15090 26.885 305 212 3 222 526 384 677 1.53e-31 132
MS.gene21622.t1 AT4G15090 28.111 217 152 4 4 217 48 263 2.79e-20 96.3
MS.gene21622.t1 AT4G15090 26.885 305 212 3 222 526 384 677 1.53e-31 132
MS.gene21622.t1 AT4G15090 28.111 217 152 4 4 217 48 263 2.79e-20 96.3
MS.gene21622.t1 AT4G15090 26.885 305 212 3 222 526 384 677 1.53e-31 132
MS.gene21622.t1 AT4G15090 28.111 217 152 4 4 217 48 263 2.79e-20 96.3
MS.gene21622.t1 AT4G15090 26.885 305 212 3 222 526 384 677 1.53e-31 132
MS.gene21622.t1 AT4G15090 28.111 217 152 4 4 217 48 263 2.79e-20 96.3
MS.gene21622.t1 AT4G15090 26.885 305 212 3 222 526 384 677 1.53e-31 132
MS.gene21622.t1 AT4G15090 28.111 217 152 4 4 217 48 263 2.79e-20 96.3
MS.gene21622.t1 AT4G38170 28.287 251 170 4 204 453 158 399 3.50e-28 120
MS.gene21622.t1 AT2G27110 26.429 280 194 5 222 500 355 623 3.66e-24 108
MS.gene21622.t1 AT2G27110 25.909 220 122 6 6 217 48 234 2.03e-11 68.2
MS.gene21622.t1 AT2G27110 26.429 280 194 5 222 500 355 623 3.66e-24 108
MS.gene21622.t1 AT2G27110 25.909 220 122 6 6 217 48 234 2.03e-11 68.2
MS.gene21622.t1 AT2G27110 26.429 280 194 5 222 500 210 478 4.48e-24 108
MS.gene21622.t1 AT1G76320 26.481 287 194 6 214 500 317 586 1.34e-23 106
MS.gene21622.t1 AT1G76320 30.909 220 136 7 11 227 1 207 4.41e-20 95.5
MS.gene21622.t1 AT1G76320 26.481 287 194 6 214 500 317 586 1.43e-23 106
MS.gene21622.t1 AT1G76320 30.909 220 136 7 11 227 1 207 4.70e-20 95.5
MS.gene21622.t1 AT1G76320 26.481 287 194 6 214 500 317 586 1.43e-23 106
MS.gene21622.t1 AT1G76320 30.909 220 136 7 11 227 1 207 4.70e-20 95.5
MS.gene21622.t1 AT1G76320 26.481 287 194 6 214 500 317 586 2.65e-23 105
MS.gene21622.t1 AT1G76320 30.909 220 136 7 11 227 1 207 3.02e-20 95.9
MS.gene21622.t1 AT4G19990 28.475 295 191 5 222 510 332 612 9.15e-22 100
MS.gene21622.t1 AT4G19990 28.475 295 191 5 222 510 332 612 9.15e-22 100
MS.gene21622.t1 AT4G19990 28.475 295 191 5 222 510 332 612 1.03e-21 100
MS.gene21622.t1 AT4G19990 28.475 295 191 5 222 510 332 612 1.03e-21 100
MS.gene21622.t1 AT4G19990 28.475 295 191 5 222 510 275 555 1.17e-21 100
MS.gene21622.t1 AT4G19990 28.475 295 191 5 222 510 332 612 1.31e-21 100
MS.gene21622.t1 AT4G19990 28.475 295 191 5 222 510 346 626 1.39e-21 100
MS.gene21622.t1 AT5G18960 29.046 241 143 7 222 452 505 727 1.53e-21 100
MS.gene21622.t1 AT5G18960 26.991 226 121 8 6 226 210 396 1.25e-12 72.0
MS.gene21622.t1 AT5G18960 29.046 241 143 7 222 452 505 727 1.53e-21 100
MS.gene21622.t1 AT5G18960 26.991 226 121 8 6 226 210 396 1.25e-12 72.0
MS.gene21622.t1 AT3G06250 29.832 238 145 7 222 452 481 703 1.96e-21 100
MS.gene21622.t1 AT3G06250 27.907 215 112 7 6 217 189 363 1.18e-13 75.1
MS.gene21622.t1 AT3G06250 29.832 238 145 7 222 452 481 703 1.96e-21 100
MS.gene21622.t1 AT3G06250 27.907 215 112 7 6 217 189 363 1.18e-13 75.1
MS.gene21622.t1 AT3G06250 29.832 238 145 7 222 452 481 703 1.96e-21 100
MS.gene21622.t1 AT3G06250 27.907 215 112 7 6 217 189 363 1.18e-13 75.1
MS.gene21622.t1 AT2G32250 23.647 351 239 8 176 513 274 608 4.11e-21 99.0
MS.gene21622.t1 AT2G32250 23.647 351 239 8 176 513 274 608 4.11e-21 99.0
MS.gene21622.t1 AT2G32250 23.647 351 239 8 176 513 233 567 4.43e-21 99.0
MS.gene21622.t1 AT2G32250 23.647 351 239 8 176 513 274 608 4.61e-21 99.0
MS.gene21622.t1 AT2G32250 23.647 351 239 8 176 513 274 608 4.61e-21 99.0
MS.gene21622.t1 AT2G32250 23.647 351 239 8 176 513 274 608 4.61e-21 99.0
MS.gene21622.t1 AT2G32250 23.647 351 239 8 176 513 233 567 5.02e-21 98.6
MS.gene21622.t1 AT2G32250 23.647 351 239 8 176 513 274 608 7.58e-21 98.2
MS.gene21622.t1 AT1G52520 22.337 291 209 6 226 507 408 690 2.39e-20 96.3
MS.gene21622.t1 AT1G80010 24.823 282 196 9 222 496 429 701 9.72e-19 91.3
MS.gene21622.t1 AT3G59470 42.718 103 56 3 6 106 69 170 1.46e-14 74.7
MS.gene21622.t1 AT3G59470 42.718 103 56 3 6 106 69 170 1.46e-14 74.7
MS.gene21622.t1 AT3G59470 42.718 103 56 3 6 106 81 182 2.19e-14 74.3
MS.gene21622.t1 AT5G28530 25.234 214 149 5 235 448 414 616 2.81e-14 77.0
MS.gene21622.t1 AT5G28530 27.647 170 117 3 5 168 53 222 2.78e-12 70.5
MS.gene21622.t1 AT5G28530 25.234 214 149 5 235 448 351 553 3.48e-14 76.6
MS.gene21622.t1 AT5G28530 27.647 170 117 3 5 168 53 222 2.32e-12 70.9
MS.gene21622.t1 AT1G10240 26.210 248 166 7 214 456 375 610 3.98e-14 76.6
MS.gene21622.t1 AT1G10240 25.792 221 151 7 7 217 48 265 3.46e-12 70.1
MS.gene21622.t1 AT1G10240 26.210 248 166 7 214 456 375 610 3.98e-14 76.6
MS.gene21622.t1 AT1G10240 25.792 221 151 7 7 217 48 265 3.46e-12 70.1
MS.gene21622.t1 AT2G43280 36.154 130 76 5 7 131 22 149 2.94e-13 69.7
MS.gene21622.t1 AT5G28530 23.364 214 149 4 235 448 414 612 6.71e-13 72.4
MS.gene21622.t1 AT5G28530 27.647 170 117 3 5 168 53 222 2.72e-12 70.5
MS.gene21622.t1 AT5G28530 23.364 214 149 4 235 448 414 612 6.71e-13 72.4
MS.gene21622.t1 AT5G28530 27.647 170 117 3 5 168 53 222 2.72e-12 70.5
MS.gene21622.t1 AT5G28530 23.364 214 149 4 235 448 351 549 8.62e-13 72.0
MS.gene21622.t1 AT5G28530 27.647 170 117 3 5 168 53 222 2.41e-12 70.9
MS.gene21622.t1 AT2G43280 35.537 121 71 5 22 137 2 120 7.08e-11 62.0

Find 125 sgRNAs with CRISPR-Local

Find 209 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAAGGAGAGTGAATGTTTC+TGG 0.114899 5.2:+3497826 None:intergenic
GAATATGGGTGGAAAATTTA+TGG 0.170586 5.2:-3499396 MS.gene21622:CDS
TAATTGATTGTGTTATTTCT+AGG 0.228763 5.2:-3498155 MS.gene21622:CDS
ATGGCGGAAGAGTTGGATTT+GGG 0.244063 5.2:-3499580 MS.gene21622:CDS
AAAGTTGAACATTAAGAATA+TGG 0.247737 5.2:-3499411 MS.gene21622:CDS
TACCATTGTATAACTCATAT+TGG 0.265727 5.2:+3497327 None:intergenic
TGTCGTAAGTTTGAGAGTTT+TGG 0.285282 5.2:-3498069 MS.gene21622:CDS
AGAACTCTCCTTGTGAAGTA+TGG 0.286425 5.2:-3498579 MS.gene21622:CDS
CCCAGAACACATTGGTGATT+TGG 0.305548 5.2:+3499036 None:intergenic
AGAAATAACACAATCAATTA+AGG 0.308077 5.2:+3498158 None:intergenic
TTCACTTTGCAAATACTTGT+TGG 0.315110 5.2:+3497754 None:intergenic
AGTCTCCTGTCTCGGATTGA+TGG 0.315178 5.2:+3499451 None:intergenic
AATGACACTCTAAATCAATT+TGG 0.318679 5.2:-3497186 MS.gene21622:CDS
ACCAAATCACCAATGTGTTC+TGG 0.321922 5.2:-3499037 MS.gene21622:CDS
TCTTTAGTCTCGGAATCTTT+TGG 0.332290 5.2:+3498304 None:intergenic
GTCTGCGAAAACCGGATAAA+CGG 0.335814 5.2:-3499484 MS.gene21622:CDS
TATGGCGGAAGAGTTGGATT+TGG 0.342801 5.2:-3499581 MS.gene21622:CDS
CAACATTGTATAACTCATAT+TGG 0.344940 5.2:+3497387 None:intergenic
TGTACTCTATAAAGGAGAAA+TGG 0.345188 5.2:-3498512 MS.gene21622:CDS
TATAGAAAATGGAAGAAGAT+TGG 0.346925 5.2:-3499664 None:intergenic
CAGAGCAATATATCTTAAAA+AGG 0.347668 5.2:-3497990 MS.gene21622:CDS
TGTATAACTCATATTGGGTT+TGG 0.348061 5.2:+3497333 None:intergenic
CTTCAGCAGCTATCCTAATA+AGG 0.348433 5.2:+3497860 None:intergenic
CTCAAAAGCTTCAAAAGTTC+AGG 0.350160 5.2:-3497581 MS.gene21622:intron
ACCATTGTATAACTCATATT+GGG 0.352366 5.2:+3497328 None:intergenic
GTGGAGAGTGTCATATGATT+TGG 0.353135 5.2:-3498115 MS.gene21622:CDS
AGGGAGAGGGGTATGGTAGG+TGG 0.363397 5.2:-3499146 MS.gene21622:CDS
CCTTGTGAAGTATGGGGAAA+TGG 0.368320 5.2:-3498571 MS.gene21622:CDS
GTTGCTAAAGTTAAAGAGAT+TGG 0.375112 5.2:-3497724 MS.gene21622:CDS
CAACATTGTATACCTCATAT+TGG 0.381744 5.2:+3497447 None:intergenic
GCAAAGAATGTACTCTATAA+AGG 0.384169 5.2:-3498520 MS.gene21622:CDS
ATCTGATTGTAGCGTTGCAC+TGG 0.395150 5.2:+3497892 None:intergenic
AGAATTATCTCCGGAAGAAA+AGG 0.399516 5.2:-3499166 MS.gene21622:CDS
AAGTTTCTAATGAATTGAAT+AGG 0.405898 5.2:-3497801 MS.gene21622:CDS
TAATGAATTGAATAGGCATT+TGG 0.408339 5.2:-3497794 MS.gene21622:CDS
TCTGCGAAAACCGGATAAAC+GGG 0.410525 5.2:-3499483 MS.gene21622:CDS
CCAAATCACCAATGTGTTCT+GGG 0.414832 5.2:-3499036 MS.gene21622:CDS
GGAGAGGGGTATGGTAGGTG+GGG 0.416084 5.2:-3499144 MS.gene21622:CDS
GGGAGAGGGGTATGGTAGGT+GGG 0.417563 5.2:-3499145 MS.gene21622:CDS
CTTTAAGCGCTTTGAGGATG+TGG 0.418127 5.2:-3498373 MS.gene21622:CDS
TCTTAGCAATTCTCAACCAT+TGG 0.421743 5.2:-3496123 MS.gene21622:intron
TAATTCTTACTATCTGCAAA+AGG 0.423881 5.2:+3496048 None:intergenic
GTACTCTATAAAGGAGAAAT+GGG 0.424286 5.2:-3498511 MS.gene21622:CDS
ATTCTCAACCATTGGATTGA+AGG 0.427234 5.2:-3496115 MS.gene21622:CDS
AAGAACAATTTGAAGAAGAA+TGG 0.434308 5.2:-3498602 MS.gene21622:CDS
AATGATTATGGCGGAAGAGT+TGG 0.440643 5.2:-3499587 MS.gene21622:CDS
CAAGTTCTTTAAGCGCTTTG+AGG 0.442444 5.2:-3498379 MS.gene21622:CDS
TAAAGAAGGTCTGCGAAAAC+CGG 0.442445 5.2:-3499492 MS.gene21622:CDS
CTTACGACAGGAGCAGGAAA+TGG 0.444862 5.2:+3498083 None:intergenic
ATGTTTGTTTGTTCTAAAGA+AGG 0.445537 5.2:-3499506 MS.gene21622:CDS
TTGATAGCGTTGAAGATGCT+TGG 0.447091 5.2:-3499619 MS.gene21622:CDS
AATTGATTGTGTTATTTCTA+GGG 0.448337 5.2:-3498154 MS.gene21622:CDS
TTTCTCGTGTATGTAAATCA+TGG 0.453503 5.2:+3499348 None:intergenic
TTGGAATTGATCGAAAATAG+TGG 0.457774 5.2:+3498236 None:intergenic
TTTCTATCATGCATATCAAA+TGG 0.460500 5.2:-3499069 MS.gene21622:CDS
CTCAAACTTACGACAGGAGC+AGG 0.464155 5.2:+3498077 None:intergenic
GAAGAGTTGGATTTGGGGTG+CGG 0.464909 5.2:-3499574 MS.gene21622:CDS
AGAACAAACAAACATGTAAG+AGG 0.465472 5.2:+3499514 None:intergenic
AGTGAGAGTTTATCTAATGT+TGG 0.475157 5.2:-3497949 MS.gene21622:CDS
TACTATCTGCAAAAGGGATG+TGG 0.476386 5.2:+3496055 None:intergenic
ATTGATTTAGCTGATGATGC+TGG 0.485268 5.2:-3499272 MS.gene21622:CDS
AATTCTTACTATCTGCAAAA+GGG 0.488779 5.2:+3496049 None:intergenic
TGATATGCATGATAGAAAGT+TGG 0.490213 5.2:+3499074 None:intergenic
AAAAGGGAGAGGGGTATGGT+AGG 0.490815 5.2:-3499149 MS.gene21622:CDS
AGTGGGAGGTAGTACTAACT+TGG 0.496217 5.2:-3499210 MS.gene21622:CDS
GAACTCTCCTTGTGAAGTAT+GGG 0.497618 5.2:-3498578 MS.gene21622:CDS
TCAACCATTGGATTGAAGGC+TGG 0.499937 5.2:-3496111 MS.gene21622:CDS
GAAGAAAAGGGAGAGGGGTA+TGG 0.506568 5.2:-3499153 MS.gene21622:CDS
TAAAACCATCAATCCGAGAC+AGG 0.509507 5.2:-3499456 MS.gene21622:CDS
ATTGGCTTACCACATTGACT+TGG 0.512775 5.2:+3497282 None:intergenic
AATGTGTTCTGGGCAGATGC+AGG 0.518530 5.2:-3499026 MS.gene21622:intron
AGAAGATTGGAAACCAAAGT+TGG 0.520792 5.2:-3499651 MS.gene21622:CDS
CCATTTCCCCATACTTCACA+AGG 0.526096 5.2:+3498571 None:intergenic
GATGGTTCGTCAAAAGCAAA+AGG 0.526235 5.2:-3497691 MS.gene21622:CDS
CAAACAACTCATACTCATGT+TGG 0.529127 5.2:+3498217 None:intergenic
CTCTCCAGCCTTCAATCCAA+TGG 0.531084 5.2:+3496107 None:intergenic
ACAAGACCATGTCCAATATG+AGG 0.537248 5.2:-3497459 MS.gene21622:CDS
GAAGTATGGGGAAATGGAAA+AGG 0.537608 5.2:-3498565 MS.gene21622:CDS
GGGAGAGTGATTTGCAAGAG+TGG 0.538572 5.2:-3498134 MS.gene21622:CDS
TGACTTAATGAGCAAAGAAG+TGG 0.538680 5.2:-3499228 MS.gene21622:CDS
AAGTTGAACATTAAGAATAT+GGG 0.540265 5.2:-3499410 MS.gene21622:CDS
TCTGATTGTAGCGTTGCACT+GGG 0.540343 5.2:+3497893 None:intergenic
GAATATGAGTTCTTTAGTCT+CGG 0.540823 5.2:+3498294 None:intergenic
TGGATTGAAGGCTGGAGAGA+TGG 0.545137 5.2:-3496103 MS.gene21622:CDS
GAATTATCTCCGGAAGAAAA+GGG 0.546525 5.2:-3499165 MS.gene21622:CDS
TTTGTCCTAGTCTCCTGTCT+CGG 0.548353 5.2:+3499443 None:intergenic
AGCAGCTATCCTAATAAGGT+GGG 0.548848 5.2:+3497864 None:intergenic
GATGGCAGGAGGTATAATGC+AGG 0.551144 5.2:-3496085 MS.gene21622:CDS
TTGATCAAAAGAATTATCTC+CGG 0.552966 5.2:-3499175 MS.gene21622:CDS
TGGGGAAATGGAAAAGGAGA+AGG 0.557328 5.2:-3498559 MS.gene21622:CDS
TTGAAGGCTGGAGAGATGGC+AGG 0.564290 5.2:-3496099 MS.gene21622:CDS
CAGCAGCTATCCTAATAAGG+TGG 0.565554 5.2:+3497863 None:intergenic
GAGATTGGAAGTAGCAGTGA+TGG 0.566239 5.2:-3497709 MS.gene21622:CDS
TGGCGGAAGAGTTGGATTTG+GGG 0.568293 5.2:-3499579 MS.gene21622:CDS
GACTTAATGAGCAAAGAAGT+GGG 0.580345 5.2:-3499227 MS.gene21622:CDS
ATTCATTAGAAACTTTGCAA+AGG 0.582265 5.2:+3497809 None:intergenic
TGGAATTGATCGAAAATAGT+GGG 0.585255 5.2:+3498237 None:intergenic
TTGAACATTAAGAATATGGG+TGG 0.586016 5.2:-3499407 MS.gene21622:CDS
GGTATGGTAGGTGGGGAAGT+CGG 0.586086 5.2:-3499137 MS.gene21622:CDS
AAAACTCTCAAACTTACGAC+AGG 0.587500 5.2:+3498071 None:intergenic
TCAATCCGAGACAGGAGACT+AGG 0.591368 5.2:-3499448 MS.gene21622:CDS
GCAGCTATCCTAATAAGGTG+GGG 0.604473 5.2:+3497865 None:intergenic
TTATACAATGGTACATACAA+TGG 0.610770 5.2:-3497317 MS.gene21622:CDS
CAGCTATCCTAATAAGGTGG+GGG 0.615168 5.2:+3497866 None:intergenic
GGCAGGAGGTATAATGCAGG+AGG 0.619602 5.2:-3496082 MS.gene21622:CDS
TCTCCGGAAGAAAAGGGAGA+GGG 0.620062 5.2:-3499159 MS.gene21622:CDS
ATCTCCGGAAGAAAAGGGAG+AGG 0.630371 5.2:-3499160 MS.gene21622:CDS
ACCCAATATGAGTTATACAA+TGG 0.639187 5.2:-3497329 MS.gene21622:CDS
GTGGGAGGTAGTACTAACTT+GGG 0.641632 5.2:-3499209 MS.gene21622:CDS
AACTCTCCTTGTGAAGTATG+GGG 0.648133 5.2:-3498577 MS.gene21622:CDS
CTCCGGAAGAAAAGGGAGAG+GGG 0.648734 5.2:-3499158 MS.gene21622:CDS
TAATGTTGGTGTTAGTAATG+TGG 0.650819 5.2:-3497935 MS.gene21622:CDS
TTATACAATGTTGCATACAA+TGG 0.654389 5.2:-3497377 MS.gene21622:CDS
TGTATACCTCATATTGGACA+TGG 0.659711 5.2:+3497453 None:intergenic
TGCATCTGCCCAGAACACAT+TGG 0.663536 5.2:+3499028 None:intergenic
GCAGGAGGTATAATGCAGGA+GGG 0.670524 5.2:-3496081 MS.gene21622:CDS
TATGGGTGGAAAATTTATGG+TGG 0.672265 5.2:-3499393 MS.gene21622:CDS
TTCACAAAGCCAAGTCAATG+TGG 0.675725 5.2:-3497291 MS.gene21622:intron
CAGGAGGTATAATGCAGGAG+GGG 0.682293 5.2:-3496080 MS.gene21622:CDS
ATGACAGAGGTGATTCACAA+AGG 0.688599 5.2:-3497415 MS.gene21622:CDS
AAGGCTGGAGAGATGGCAGG+AGG 0.695576 5.2:-3496096 MS.gene21622:CDS
GTTGCATACAATGGTGACAG+AGG 0.705992 5.2:-3497368 MS.gene21622:CDS
TTAATGAGCAAAGAAGTGGG+AGG 0.756656 5.2:-3499224 MS.gene21622:CDS
CTGATTGTAGCGTTGCACTG+GGG 0.768246 5.2:+3497894 None:intergenic
GTTGCATACAATGATGACAG+AGG 0.780403 5.2:-3497428 MS.gene21622:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGAGAAAATTAATAAGAAAA+TGG + chr5.2:3499563-3499582 None:intergenic 15.0%
!!! ATTAAATTTTTCTTCTTGTA+TGG - chr5.2:3498197-3498216 MS.gene21622:CDS 15.0%
!! AAAGTTGAACATTAAGAATA+TGG - chr5.2:3496292-3496311 MS.gene21622:intron 20.0%
!! AAGTTGAACATTAAGAATAT+GGG - chr5.2:3496293-3496312 MS.gene21622:intron 20.0%
!! AAGTTTCTAATGAATTGAAT+AGG - chr5.2:3497902-3497921 MS.gene21622:CDS 20.0%
!! AATTTAATACTACATGTAGT+TGG + chr5.2:3498185-3498204 None:intergenic 20.0%
!! AGAAATAACACAATCAATTA+AGG + chr5.2:3497548-3497567 None:intergenic 20.0%
!! ATAATACTTCTCTAAATAGT+TGG + chr5.2:3498803-3498822 None:intergenic 20.0%
!! ATTGTACTTAATTTAGATAG+AGG - chr5.2:3498773-3498792 MS.gene21622:intron 20.0%
!! TTTAAATGTTTCCTAAAATG+AGG - chr5.2:3499063-3499082 MS.gene21622:CDS 20.0%
!!! AAATTTTACAATGATTATGG+CGG - chr5.2:3496107-3496126 MS.gene21622:CDS 20.0%
!!! AATGATTTTATCTTTTATGC+AGG - chr5.2:3498489-3498508 MS.gene21622:CDS 20.0%
!!! AATTGATTGTGTTATTTCTA+GGG - chr5.2:3497549-3497568 MS.gene21622:intron 20.0%
!!! TAATTGATTGTGTTATTTCT+AGG - chr5.2:3497548-3497567 MS.gene21622:intron 20.0%
!!! TGGAAATTTTACAATGATTA+TGG - chr5.2:3496104-3496123 MS.gene21622:CDS 20.0%
! AAAGGCTTCAAAAAAAAAGA+AGG - chr5.2:3498030-3498049 MS.gene21622:CDS 25.0%
! AAGAACAATTTGAAGAAGAA+TGG - chr5.2:3497101-3497120 MS.gene21622:intron 25.0%
! AATGACACTCTAAATCAATT+TGG - chr5.2:3498517-3498536 MS.gene21622:CDS 25.0%
! AATTCTTACTATCTGCAAAA+GGG + chr5.2:3499657-3499676 None:intergenic 25.0%
! ACCATTGTATAACTCATATT+GGG + chr5.2:3498378-3498397 None:intergenic 25.0%
! AGCAATATATCTTAAAAAGG+TGG - chr5.2:3497716-3497735 MS.gene21622:CDS 25.0%
! ATACTTTCAGAAAAAATTGG+TGG - chr5.2:3496601-3496620 MS.gene21622:intron 25.0%
! ATTCATTAGAAACTTTGCAA+AGG + chr5.2:3497897-3497916 None:intergenic 25.0%
! CAACATTGTATAACTCATAT+TGG + chr5.2:3498319-3498338 None:intergenic 25.0%
! CACTTAGGAAATTTAATGAA+AGG - chr5.2:3497025-3497044 MS.gene21622:intron 25.0%
! CAGAGCAATATATCTTAAAA+AGG - chr5.2:3497713-3497732 MS.gene21622:CDS 25.0%
! GCAATACTTTCAGAAAAAAT+TGG - chr5.2:3496598-3496617 MS.gene21622:intron 25.0%
! GTACAATCTCAAAATAACAT+AGG + chr5.2:3498760-3498779 None:intergenic 25.0%
! TAAAAACCCAAAAAAGAACA+TGG + chr5.2:3498859-3498878 None:intergenic 25.0%
! TAAATCACGTCATTAGAAAT+TGG + chr5.2:3498596-3498615 None:intergenic 25.0%
! TAATGAATTGAATAGGCATT+TGG - chr5.2:3497909-3497928 MS.gene21622:CDS 25.0%
! TAATTCTTACTATCTGCAAA+AGG + chr5.2:3499658-3499677 None:intergenic 25.0%
! TACCATTGTATAACTCATAT+TGG + chr5.2:3498379-3498398 None:intergenic 25.0%
! TAGGAAATTTAATGAAAGGT+GGG - chr5.2:3497029-3497048 MS.gene21622:intron 25.0%
! TATTCACACAAGAACAAAAA+TGG - chr5.2:3496158-3496177 MS.gene21622:intron 25.0%
! TCAAAAATCATTTCCAACTT+TGG + chr5.2:3496068-3496087 None:intergenic 25.0%
! TTAGGAAATTTAATGAAAGG+TGG - chr5.2:3497028-3497047 MS.gene21622:intron 25.0%
! TTATACAATGGTACATACAA+TGG - chr5.2:3498386-3498405 MS.gene21622:CDS 25.0%
! TTATACAATGTTGCATACAA+TGG - chr5.2:3498326-3498345 MS.gene21622:CDS 25.0%
! TTGATCAAAAGAATTATCTC+CGG - chr5.2:3496528-3496547 MS.gene21622:intron 25.0%
! TTTCTATCATGCATATCAAA+TGG - chr5.2:3496634-3496653 MS.gene21622:intron 25.0%
!! AAACATCTGAGACATATAAA+TGG - chr5.2:3496849-3496868 MS.gene21622:intron 25.0%
!! ATGAAGAAAGCTTTTACATT+AGG - chr5.2:3497226-3497245 MS.gene21622:intron 25.0%
!! GATTTTCCATGTTCTTTTTT+GGG - chr5.2:3498850-3498869 MS.gene21622:intron 25.0%
!! TATATCACTTTCCTCATTTT+AGG + chr5.2:3499077-3499096 None:intergenic 25.0%
!! TGATTTTCCATGTTCTTTTT+TGG - chr5.2:3498849-3498868 MS.gene21622:intron 25.0%
!!! AAAGCGTTTTAAAAGTTGTT+TGG - chr5.2:3498065-3498084 MS.gene21622:CDS 25.0%
!!! ATGTTTGTTTGTTCTAAAGA+AGG - chr5.2:3496197-3496216 MS.gene21622:intron 25.0%
!!! CCATCATTTTTCTTGTTTTT+GGG + chr5.2:3498102-3498121 None:intergenic 25.0%
!!! CGTGGTTAAAACAATTTTTT+TGG + chr5.2:3499514-3499533 None:intergenic 25.0%
!!! CTTTTTAAGATATATTGCTC+TGG + chr5.2:3497715-3497734 None:intergenic 25.0%
!!! TAGTTTTACTAGACTATTGA+TGG - chr5.2:3498546-3498565 MS.gene21622:CDS 25.0%
!!! TGAGATATTTTTCTTTGAGA+TGG + chr5.2:3496395-3496414 None:intergenic 25.0%
ACAACATTGAACAACTCATA+AGG - chr5.2:3498671-3498690 MS.gene21622:intron 30.0%
ACCCAATATGAGTTATACAA+TGG - chr5.2:3498374-3498393 MS.gene21622:CDS 30.0%
AGAACAAACAAACATGTAAG+AGG + chr5.2:3496192-3496211 None:intergenic 30.0%
AGGAGAAGGAAAAAAATAGT+TGG - chr5.2:3497158-3497177 MS.gene21622:CDS 30.0%
AGTGAGAGTTTATCTAATGT+TGG - chr5.2:3497754-3497773 MS.gene21622:CDS 30.0%
CAACATTGTATACCTCATAT+TGG + chr5.2:3498259-3498278 None:intergenic 30.0%
CCCAAAAACAAGAAAAATGA+TGG - chr5.2:3498099-3498118 MS.gene21622:CDS 30.0%
GAATATGAGTTCTTTAGTCT+CGG + chr5.2:3497412-3497431 None:intergenic 30.0%
GAATATGGGTGGAAAATTTA+TGG - chr5.2:3496307-3496326 MS.gene21622:intron 30.0%
GCAAAGAATGTACTCTATAA+AGG - chr5.2:3497183-3497202 MS.gene21622:CDS 30.0%
GTACTCTATAAAGGAGAAAT+GGG - chr5.2:3497192-3497211 MS.gene21622:CDS 30.0%
GTTGCTAAAGTTAAAGAGAT+TGG - chr5.2:3497979-3497998 MS.gene21622:CDS 30.0%
TACTGATCAAGATCAATCAA+TGG - chr5.2:3496925-3496944 MS.gene21622:intron 30.0%
TCACATACTCATACTGTTAT+TGG + chr5.2:3498442-3498461 None:intergenic 30.0%
TGATATGCATGATAGAAAGT+TGG + chr5.2:3496632-3496651 None:intergenic 30.0%
TGATCAGTAAAAACTGTTTG+AGG + chr5.2:3496914-3496933 None:intergenic 30.0%
TGGAATTGATCGAAAATAGT+GGG + chr5.2:3497469-3497488 None:intergenic 30.0%
TGTACTCTATAAAGGAGAAA+TGG - chr5.2:3497191-3497210 MS.gene21622:CDS 30.0%
TGTATAACTCATATTGGGTT+TGG + chr5.2:3498373-3498392 None:intergenic 30.0%
TTCACTTTGCAAATACTTGT+TGG + chr5.2:3497952-3497971 None:intergenic 30.0%
TTGAACATTAAGAATATGGG+TGG - chr5.2:3496296-3496315 MS.gene21622:intron 30.0%
TTGGAATTGATCGAAAATAG+TGG + chr5.2:3497470-3497489 None:intergenic 30.0%
TTGTAGAATTGTCAAAATCG+CGG - chr5.2:3499235-3499254 MS.gene21622:CDS 30.0%
TTGTCCAATTGTTGAAATTG+CGG - chr5.2:3499284-3499303 MS.gene21622:CDS 30.0%
TTGTTGAATTGTCAAAATCG+CGG - chr5.2:3499333-3499352 MS.gene21622:CDS 30.0%
TTTCTCGTGTATGTAAATCA+TGG + chr5.2:3496358-3496377 None:intergenic 30.0%
! GTTTTATAATCCCGTTTATC+CGG + chr5.2:3496233-3496252 None:intergenic 30.0%
!! ATTTATGGTGGTTGATTTTG+TGG - chr5.2:3496322-3496341 MS.gene21622:intron 30.0%
!! CTCATTGATTATGAGTGTTT+TGG - chr5.2:3496704-3496723 MS.gene21622:intron 30.0%
!! TAATGTTGGTGTTAGTAATG+TGG - chr5.2:3497768-3497787 MS.gene21622:CDS 30.0%
!! TTTTAAAAGTTGTTTGGAGC+GGG - chr5.2:3498071-3498090 MS.gene21622:CDS 30.0%
!!! GCCATCATTTTTCTTGTTTT+TGG + chr5.2:3498103-3498122 None:intergenic 30.0%
!!! GTTTTAAAAGTTGTTTGGAG+CGG - chr5.2:3498070-3498089 MS.gene21622:CDS 30.0%
AAAACTCTCAAACTTACGAC+AGG + chr5.2:3497635-3497654 None:intergenic 35.0%
AAGAGAGAGAAAACAAAACG+TGG + chr5.2:3499532-3499551 None:intergenic 35.0%
AATGTTGTGACACTGTATTC+AGG + chr5.2:3498712-3498731 None:intergenic 35.0%
ACAACATTGACCAACTCATA+AGG - chr5.2:3498724-3498743 MS.gene21622:intron 35.0%
ACTTATGTCTCTGCAATTGT+AGG - chr5.2:3498637-3498656 MS.gene21622:CDS 35.0%
AGAAGATTGGAAACCAAAGT+TGG - chr5.2:3496052-3496071 MS.gene21622:CDS 35.0%
AGAATTATCTCCGGAAGAAA+AGG - chr5.2:3496537-3496556 MS.gene21622:intron 35.0%
ATTCTCAACCATTGGATTGA+AGG - chr5.2:3499588-3499607 MS.gene21622:CDS 35.0%
CAAACAACTCATACTCATGT+TGG + chr5.2:3497489-3497508 None:intergenic 35.0%
CTCAAAAGCTTCAAAAGTTC+AGG - chr5.2:3498122-3498141 MS.gene21622:CDS 35.0%
GAATTATCTCCGGAAGAAAA+GGG - chr5.2:3496538-3496557 MS.gene21622:intron 35.0%
GACTTAATGAGCAAAGAAGT+GGG - chr5.2:3496476-3496495 MS.gene21622:intron 35.0%
TATGGGTGGAAAATTTATGG+TGG - chr5.2:3496310-3496329 MS.gene21622:intron 35.0%
TCTTAGCAATTCTCAACCAT+TGG - chr5.2:3499580-3499599 MS.gene21622:CDS 35.0%
TGACTTAATGAGCAAAGAAG+TGG - chr5.2:3496475-3496494 MS.gene21622:intron 35.0%
TGATTCACATCCTTATGAGT+TGG + chr5.2:3498684-3498703 None:intergenic 35.0%
TGTATACCTCATATTGGACA+TGG + chr5.2:3498253-3498272 None:intergenic 35.0%
! ATTGATTTAGCTGATGATGC+TGG - chr5.2:3496431-3496450 MS.gene21622:intron 35.0%
! TCTTTAGTCTCGGAATCTTT+TGG + chr5.2:3497402-3497421 None:intergenic 35.0%
! TGTCGTAAGTTTGAGAGTTT+TGG - chr5.2:3497634-3497653 MS.gene21622:CDS 35.0%
!! CTTTGAAATTGTCGAAATCG+CGG - chr5.2:3499188-3499207 MS.gene21622:CDS 35.0%
!!! GCTCTTTGAGACATTTTTAG+AGG - chr5.2:3496871-3496890 MS.gene21622:intron 35.0%
AACGCCGCAATTTCAACAAT+TGG + chr5.2:3499291-3499310 None:intergenic 40.0%
AACTCTCCTTGTGAAGTATG+GGG - chr5.2:3497126-3497145 MS.gene21622:intron 40.0%
AATGGCAAACGCATTGAATG+AGG - chr5.2:3496943-3496962 MS.gene21622:intron 40.0%
ACAAGACCATGTCCAATATG+AGG - chr5.2:3498244-3498263 MS.gene21622:CDS 40.0%
ACCAAATCACCAATGTGTTC+TGG - chr5.2:3496666-3496685 MS.gene21622:intron 40.0%
AGAACTCTCCTTGTGAAGTA+TGG - chr5.2:3497124-3497143 MS.gene21622:intron 40.0%
AGCAGCTATCCTAATAAGGT+GGG + chr5.2:3497842-3497861 None:intergenic 40.0%
ATGACAGAGGTGATTCACAA+AGG - chr5.2:3498288-3498307 MS.gene21622:CDS 40.0%
CAAAGGAGAGTGAATGTTTC+TGG + chr5.2:3497880-3497899 None:intergenic 40.0%
CAAGTTCTTTAAGCGCTTTG+AGG - chr5.2:3497324-3497343 MS.gene21622:CDS 40.0%
CAGAATGGCATCAAACACTT+AGG - chr5.2:3497010-3497029 MS.gene21622:intron 40.0%
CCAAATCACCAATGTGTTCT+GGG - chr5.2:3496667-3496686 MS.gene21622:intron 40.0%
CTTCAGCAGCTATCCTAATA+AGG + chr5.2:3497846-3497865 None:intergenic 40.0%
GAACTCTCCTTGTGAAGTAT+GGG - chr5.2:3497125-3497144 MS.gene21622:intron 40.0%
GAAGTATGGGGAAATGGAAA+AGG - chr5.2:3497138-3497157 MS.gene21622:intron 40.0%
GATGGTTCGTCAAAAGCAAA+AGG - chr5.2:3498012-3498031 MS.gene21622:CDS 40.0%
GTGTGTGATATGCTTTCAAC+AGG - chr5.2:3499416-3499435 MS.gene21622:CDS 40.0%
GTTGCATACAATGATGACAG+AGG - chr5.2:3498275-3498294 MS.gene21622:CDS 40.0%
TAAAACCATCAATCCGAGAC+AGG - chr5.2:3496247-3496266 MS.gene21622:intron 40.0%
TAAAGAAGGTCTGCGAAAAC+CGG - chr5.2:3496211-3496230 MS.gene21622:intron 40.0%
TACTATCTGCAAAAGGGATG+TGG + chr5.2:3499651-3499670 None:intergenic 40.0%
TATGTCTCTGCAATTGTAGG+AGG - chr5.2:3498640-3498659 MS.gene21622:CDS 40.0%
TTAATGAGCAAAGAAGTGGG+AGG - chr5.2:3496479-3496498 MS.gene21622:intron 40.0%
TTCACAAAGCCAAGTCAATG+TGG - chr5.2:3498412-3498431 MS.gene21622:CDS 40.0%
TTTACCATGGCTTATGCACA+TGG - chr5.2:3496978-3496997 MS.gene21622:intron 40.0%
! AATGATTATGGCGGAAGAGT+TGG - chr5.2:3496116-3496135 MS.gene21622:CDS 40.0%
! ATCGAGGAGCAGTGATTTTT+GGG - chr5.2:3496807-3496826 MS.gene21622:intron 40.0%
! ATTGGCTTACCACATTGACT+TGG + chr5.2:3498424-3498443 None:intergenic 40.0%
!! GTGGAGAGTGTCATATGATT+TGG - chr5.2:3497588-3497607 MS.gene21622:CDS 40.0%
!! TATGGTGGTTGATTTTGTGG+AGG - chr5.2:3496325-3496344 MS.gene21622:intron 40.0%
!! TTGATAGCGTTGAAGATGCT+TGG - chr5.2:3496084-3496103 MS.gene21622:CDS 40.0%
ACATGGCACTTGATGCAGAA+TGG - chr5.2:3496995-3497014 MS.gene21622:intron 45.0%
AGATTTGCCCCCACCTTATT+AGG - chr5.2:3497830-3497849 MS.gene21622:CDS 45.0%
AGTGGGAGGTAGTACTAACT+TGG - chr5.2:3496493-3496512 MS.gene21622:intron 45.0%
ATCTGATTGTAGCGTTGCAC+TGG + chr5.2:3497814-3497833 None:intergenic 45.0%
CAAAAATCACTGCTCCTCGA+TGG + chr5.2:3496808-3496827 None:intergenic 45.0%
CAGCAGCTATCCTAATAAGG+TGG + chr5.2:3497843-3497862 None:intergenic 45.0%
CAGCTATCCTAATAAGGTGG+GGG + chr5.2:3497840-3497859 None:intergenic 45.0%
CATAAGCCATGGTAAACTCC+AGG + chr5.2:3496974-3496993 None:intergenic 45.0%
CCATTTCCCCATACTTCACA+AGG + chr5.2:3497135-3497154 None:intergenic 45.0%
CCTTGTGAAGTATGGGGAAA+TGG - chr5.2:3497132-3497151 MS.gene21622:intron 45.0%
CTCAGCAACAGGTTTGAAGA+TGG - chr5.2:3499394-3499413 MS.gene21622:CDS 45.0%
GAGATTGGAAGTAGCAGTGA+TGG - chr5.2:3497994-3498013 MS.gene21622:CDS 45.0%
GCAAAGTTGTTCTCAGCAAC+AGG - chr5.2:3499383-3499402 MS.gene21622:CDS 45.0%
GCAGCTATCCTAATAAGGTG+GGG + chr5.2:3497841-3497860 None:intergenic 45.0%
GCATTGAATGAGGTTATGCC+TGG - chr5.2:3496953-3496972 MS.gene21622:intron 45.0%
GTCTGCGAAAACCGGATAAA+CGG - chr5.2:3496219-3496238 MS.gene21622:intron 45.0%
GTGGGAGGTAGTACTAACTT+GGG - chr5.2:3496494-3496513 MS.gene21622:intron 45.0%
GTTATGCCTGGAGTTTACCA+TGG - chr5.2:3496965-3496984 MS.gene21622:intron 45.0%
GTTGCATACAATGGTGACAG+AGG - chr5.2:3498335-3498354 MS.gene21622:CDS 45.0%
TAGACCACTTGCAGTGTTCT+CGG - chr5.2:3496772-3496791 MS.gene21622:intron 45.0%
TCAACCATTGGATTGAAGGC+TGG - chr5.2:3499592-3499611 MS.gene21622:CDS 45.0%
TCTGATTGTAGCGTTGCACT+GGG + chr5.2:3497813-3497832 None:intergenic 45.0%
TCTGCGAAAACCGGATAAAC+GGG - chr5.2:3496220-3496239 MS.gene21622:intron 45.0%
TGGGGAAATGGAAAAGGAGA+AGG - chr5.2:3497144-3497163 MS.gene21622:intron 45.0%
TTTGTCCTAGTCTCCTGTCT+CGG + chr5.2:3496263-3496282 None:intergenic 45.0%
! ATGGCGGAAGAGTTGGATTT+GGG - chr5.2:3496123-3496142 MS.gene21622:intron 45.0%
! CATCGAGGAGCAGTGATTTT+TGG - chr5.2:3496806-3496825 MS.gene21622:intron 45.0%
! CCCAGAACACATTGGTGATT+TGG + chr5.2:3496670-3496689 None:intergenic 45.0%
! TATGGCGGAAGAGTTGGATT+TGG - chr5.2:3496122-3496141 MS.gene21622:intron 45.0%
! TCGAGGAGCAGTGATTTTTG+GGG - chr5.2:3496808-3496827 MS.gene21622:intron 45.0%
!! CTTTAAGCGCTTTGAGGATG+TGG - chr5.2:3497330-3497349 MS.gene21622:CDS 45.0%
AAATCACTGCTCCTCGATGG+TGG + chr5.2:3496805-3496824 None:intergenic 50.0%
AATCCCGAGAACACTGCAAG+TGG + chr5.2:3496779-3496798 None:intergenic 50.0%
ACGACTTCAACAGCAGCAAG+AGG + chr5.2:3499164-3499183 None:intergenic 50.0%
AGACCACTTGCAGTGTTCTC+GGG - chr5.2:3496773-3496792 MS.gene21622:intron 50.0%
AGTCTCCTGTCTCGGATTGA+TGG + chr5.2:3496255-3496274 None:intergenic 50.0%
AGTGCCATGTGCATAAGCCA+TGG + chr5.2:3496985-3497004 None:intergenic 50.0%
ATCTCCGGAAGAAAAGGGAG+AGG - chr5.2:3496543-3496562 MS.gene21622:intron 50.0%
CTCAAACTTACGACAGGAGC+AGG + chr5.2:3497629-3497648 None:intergenic 50.0%
CTCTCCAGCCTTCAATCCAA+TGG + chr5.2:3499599-3499618 None:intergenic 50.0%
CTGATTGTAGCGTTGCACTG+GGG + chr5.2:3497812-3497831 None:intergenic 50.0%
CTTACGACAGGAGCAGGAAA+TGG + chr5.2:3497623-3497642 None:intergenic 50.0%
GAAGAAAAGGGAGAGGGGTA+TGG - chr5.2:3496550-3496569 MS.gene21622:intron 50.0%
GTCTCTGCAATTGTAGGAGG+AGG - chr5.2:3498643-3498662 MS.gene21622:CDS 50.0%
TCAATCCGAGACAGGAGACT+AGG - chr5.2:3496255-3496274 MS.gene21622:intron 50.0%
TCGGGATTTAACCACCATCG+AGG - chr5.2:3496791-3496810 MS.gene21622:intron 50.0%
TCTCCGGAAGAAAAGGGAGA+GGG - chr5.2:3496544-3496563 MS.gene21622:intron 50.0%
TGCATCTGCCCAGAACACAT+TGG + chr5.2:3496678-3496697 None:intergenic 50.0%
TGGATTGAAGGCTGGAGAGA+TGG - chr5.2:3499600-3499619 MS.gene21622:CDS 50.0%
TGGCGGAAGAGTTGGATTTG+GGG - chr5.2:3496124-3496143 MS.gene21622:intron 50.0%
! AAAAGGGAGAGGGGTATGGT+AGG - chr5.2:3496554-3496573 MS.gene21622:intron 50.0%
! AATGTGTTCTGGGCAGATGC+AGG - chr5.2:3496677-3496696 MS.gene21622:intron 50.0%
! CAGGAGGTATAATGCAGGAG+GGG - chr5.2:3499623-3499642 MS.gene21622:CDS 50.0%
! GAAGAGTTGGATTTGGGGTG+CGG - chr5.2:3496129-3496148 MS.gene21622:intron 50.0%
! GATGGCAGGAGGTATAATGC+AGG - chr5.2:3499618-3499637 MS.gene21622:CDS 50.0%
! GCAGGAGGTATAATGCAGGA+GGG - chr5.2:3499622-3499641 MS.gene21622:CDS 50.0%
! GGGAGAGTGATTTGCAAGAG+TGG - chr5.2:3497569-3497588 MS.gene21622:intron 50.0%
! TACCCCTCTCCCTTTTCTTC+CGG + chr5.2:3496550-3496569 None:intergenic 50.0%
CAACAGCAGCAAGAGGCTCA+GGG + chr5.2:3499157-3499176 None:intergenic 55.0%
CTCCGGAAGAAAAGGGAGAG+GGG - chr5.2:3496545-3496564 MS.gene21622:intron 55.0%
GGTATGGTAGGTGGGGAAGT+CGG - chr5.2:3496566-3496585 MS.gene21622:intron 55.0%
TCAACAGCAGCAAGAGGCTC+AGG + chr5.2:3499158-3499177 None:intergenic 55.0%
TTGAAGGCTGGAGAGATGGC+AGG - chr5.2:3499604-3499623 MS.gene21622:CDS 55.0%
! GGCAGGAGGTATAATGCAGG+AGG - chr5.2:3499621-3499640 MS.gene21622:CDS 55.0%
AAGGCTGGAGAGATGGCAGG+AGG - chr5.2:3499607-3499626 MS.gene21622:CDS 60.0%
! AGGGAGAGGGGTATGGTAGG+TGG - chr5.2:3496557-3496576 MS.gene21622:intron 60.0%
! GGAGAGGGGTATGGTAGGTG+GGG - chr5.2:3496559-3496578 MS.gene21622:intron 60.0%
! GGGAGAGGGGTATGGTAGGT+GGG - chr5.2:3496558-3496577 MS.gene21622:intron 60.0%
AGCAGCAAGAGGCTCAGGGC+AGG + chr5.2:3499153-3499172 None:intergenic 65.0%
GCAGCAAGAGGCTCAGGGCA+GGG + chr5.2:3499152-3499171 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 3496047 3499678 3496047 ID=MS.gene21622
chr5.2 mRNA 3496047 3499678 3496047 ID=MS.gene21622.t1;Parent=MS.gene21622
chr5.2 exon 3499027 3499678 3499027 ID=MS.gene21622.t1.exon1;Parent=MS.gene21622.t1
chr5.2 CDS 3499027 3499678 3499027 ID=cds.MS.gene21622.t1;Parent=MS.gene21622.t1
chr5.2 exon 3497582 3498678 3497582 ID=MS.gene21622.t1.exon2;Parent=MS.gene21622.t1
chr5.2 CDS 3497582 3498678 3497582 ID=cds.MS.gene21622.t1;Parent=MS.gene21622.t1
chr5.2 exon 3497292 3497498 3497292 ID=MS.gene21622.t1.exon3;Parent=MS.gene21622.t1
chr5.2 CDS 3497292 3497498 3497292 ID=cds.MS.gene21622.t1;Parent=MS.gene21622.t1
chr5.2 exon 3497158 3497214 3497158 ID=MS.gene21622.t1.exon4;Parent=MS.gene21622.t1
chr5.2 CDS 3497158 3497214 3497158 ID=cds.MS.gene21622.t1;Parent=MS.gene21622.t1
chr5.2 exon 3496047 3496139 3496047 ID=MS.gene21622.t1.exon5;Parent=MS.gene21622.t1
chr5.2 CDS 3496047 3496139 3496047 ID=cds.MS.gene21622.t1;Parent=MS.gene21622.t1
Gene Sequence

>MS.gene21622

ATGGAAGAAGATTGGAAACCAAAGTTGGAAATGATTTTTGATAGCGTTGAAGATGCTTGGAAATTTTACAATGATTATGGCGGAAGAGTTGGATTTGGGGTGCGGAAACAATATTCACACAAGAACAAAAATGGTATAATTACCTCTTACATGTTTGTTTGTTCTAAAGAAGGTCTGCGAAAACCGGATAAACGGGATTATAAAACCATCAATCCGAGACAGGAGACTAGGACAAATTGTAAAGCAAAGTTGAACATTAAGAATATGGGTGGAAAATTTATGGTGGTTGATTTTGTGGAGGAACATAACCATGATTTACATACACGAGAAACAACTCATATGTTGCCATCTCAAAGAAAAATATCTCAAATTCAATGTCAGCAAATTGATTTAGCTGATGATGCTGGACTACAACAGAGAAAGTCATTTGACTTAATGAGCAAAGAAGTGGGAGGTAGTACTAACTTGGGATTTACTCGTGTTGATCAAAAGAATTATCTCCGGAAGAAAAGGGAGAGGGGTATGGTAGGTGGGGAAGTCGGTTATCTGTTGCAATACTTTCAGAAAAAATTGGTGGAAAATCCAACTTTCTATCATGCATATCAAATGGATACTGAAGACCAAATCACCAATGTGTTCTGGGCAGATGCAGGTATGCTCATTGATTATGAGTGTTTTGGTGATGTGATTTCTCTTGATTCAACATATTGCACAAATAGTTCACATAGACCACTTGCAGTGTTCTCGGGATTTAACCACCATCGAGGAGCAGTGATTTTTGGGGCTGCATTGTTGTATGACGAAACATCTGAGACATATAAATGGCTCTTTGAGACATTTTTAGAGGCTCATAAGCAGAAAATGCCTCAAACAGTTTTTACTGATCAAGATCAATCAATGGCAAACGCATTGAATGAGGTTATGCCTGGAGTTTACCATGGCTTATGCACATGGCACTTGATGCAGAATGGCATCAAACACTTAGGAAATTTAATGAAAGGTGGGTCATGTTTTCTTAGTGACTTTAAGAAGTGCATGTATGACTATGAAAATGAAGAACAATTTGAAGAAGAATGGAGAACTCTCCTTGTGAAGTATGGGGAAATGGAAAAGGAGAAGGAAAAAAATAGTTGGCTGCAAAGAATGTACTCTATAAAGGAGAAATGGGCATCATGTTACATGAAGAAAGCTTTTACATTAGGCATGCGAAGTACTCAACTAAGTGAAAGTGTGAATGCAGATATTAAAAGTTTTATCAATGTCAAGTTGAATATTATCAAGTTCTTTAAGCGCTTTGAGGATGTGGTACAACAAAAGCGAAAAAATGAATTGAAGTGTGAATATGAAGCACGCCAAAAGATTCCGAGACTAAAGAACTCATATTCACATATCTTGCAGCAAGTGTCTAAGCTTTACACTCCCACTATTTTCGATCAATTCCAACATGAGTATGAGTTGTTTGAAGCATATTTTGTGAAAAACATTAACACACAAGCAACCTTAATTGATTGTGTTATTTCTAGGGAGAGTGATTTGCAAGAGTGGAGAGTGTCATATGATTTGGATAATAATTCCATTTCCTGCTCCTGTCGTAAGTTTGAGAGTTTTGGCATACTTTGTTGTCACTGTTTACGATTATTTATTCATATGAATGTCAGATCAGTTCCAGAGCAATATATCTTAAAAAGGTGGACAAATTTAGCAAGAAGTGAGAGTTTATCTAATGTTGGTGTTAGTAATGTGGTAGAAGATGTTGATCTATCCCCAGTGCAACGCTACAATCAGATTTGCCCCCACCTTATTAGGATAGCTGCTGAAGCATGTAGAAATCCAGAAACATTCACTCTCCTTTGCAAAGTTTCTAATGAATTGAATAGGCATTTGGTAGAGTTTCAACACAATCCAACAAGTATTTGCAAAGTGAATGAGTTTGTTGCTAAAGTTAAAGAGATTGGAAGTAGCAGTGATGGTTCGTCAAAAGCAAAAGGCTTCAAAAAAAAAGAAGGCACAAGACGTACAAAGCGTTTTAAAAGTTGTTTGGAGCGGGAACTTCCCAAAAACAAGAAAAATGATGGCTCAAAAGCTTCAAAAGTTCAGGTAGTTTTAAATTACTGAATATTCATATTGTCTTTTTTCCAACTACATGTAGTATTAAATTTTTCTTCTTGTATGGCATGTAGAACTCTAAAAAGCATACACAAGACCATGTCCAATATGAGGTATACAATGTTGCATACAATGATGACAGAGGTGATTCACAAAGGCAAGTCCAATATGAGTTATACAATGTTGCATACAATGGTGACAGAGGTGATTCACAAAGCCAAACCCAATATGAGTTATACAATGGTACATACAATGGTGATTCACAAAGCCAAGTCAATGTGGTAAGCCAATAACAGTATGAGTATGTGAATGTACAAAAAATAAATTTAGTGATTAAATGATTTTATCTTTTATGCAGGATAATAATGACACTCTAAATCAATTTGGTGTTACTAGTTTTACTAGACTATTGATGGTAAGTTTTAGCAACTATATAATTTCCAATTTCTAATGACGTGATTTATCTTAATATCTACACTTATTAACTTATGTCTCTGCAATTGTAGGAGGAGGATGTCACAACATTGAACAACTCATAAGGATGTGAATCAAATATCCTGAATACAGTGTCACAACATTGACCAACTCATAAGGATGTGAATCACCTATGTTATTTTGAGATTGTACTTAATTTAGATAGAGGAACGCCAACTATTTAGAGAAGTATTATGAGAGCAATTATGTAGCAAGTACTTCTGATTTTCCATGTTCTTTTTTGGGTTTTTATGTGAGCTGAAGCAACTAGTTTAGCAAGTACTTCTGATTTTCCATGTTCTTTTTTGGGTTTTTATGTGAGCTGAAGCAACTAGTTTATGACATTGTTATGATAACAAGTTTATGCAGATGAACAAGTTTGTTGAATTGATTATTTTTCTCTCATGATACACACAAAGTCTAGCAAAACAAGTGTTTTAAATGTTTCCTAAAATGAGGAAAGTGATATAGTAAGAGTTTGCAGCAACAATGAAACATTCTTTCATTAAGAGAGAAAGAAATCCCTGCCCTGAGCCTCTTGCTGCTGTTGAAGTCGTTAAGCTTTGAAATTGTCGAAATCGCGGCGTTGTTATTAAACATTTTTGAAATTGTAGAATTGTCAAAATCGCGGCGTTGTTATTAAACAGATTTTTTAAATTGTCCAATTGTTGAAATTGCGGCGTTGTTTTTAAACAGATTTTTTAAATTGTTGAATTGTCAAAATCGCGGCGTTGTTATTAAACAGATTTTGTAGTTGCAAAGTTGTTCTCAGCAACAGGTTTGAAGATGGTGTGTGATATGCTTTCAACAGGATCTTTAAGTTGTTAAGTGTATCTATCACTCTGCTTATGAGTTATTAACAAAAAGAAAAAAAAAAACTGATTCCAAAAAAATTGTTTTAACCACGTTTTGTTTTCTCTCTCTTCAATAATGCCATTTTCTTATTAATTTTCTCTTAGCAATTCTCAACCATTGGATTGAAGGCTGGAGAGATGGCAGGAGGTATAATGCAGGAGGGGATCCACATCCCTTTTGCAGATAGTAAGAATTAA
Protein sequence

>MS.gene21622.t1

MEEDWKPKLEMIFDSVEDAWKFYNDYGGRVGFGVRKQYSHKNKNGIITSYMFVCSKEGLRKPDKRDYKTINPRQETRTNCKAKLNIKNMGGKFMVVDFVEEHNHDLHTRETTHMLPSQRKISQIQCQQIDLADDAGLQQRKSFDLMSKEVGGSTNLGFTRVDQKNYLRKKRERGMVGGEVGYLLQYFQKKLVENPTFYHAYQMDTEDQITNVFWADAGGSCFLSDFKKCMYDYENEEQFEEEWRTLLVKYGEMEKEKEKNSWLQRMYSIKEKWASCYMKKAFTLGMRSTQLSESVNADIKSFINVKLNIIKFFKRFEDVVQQKRKNELKCEYEARQKIPRLKNSYSHILQQVSKLYTPTIFDQFQHEYELFEAYFVKNINTQATLIDCVISRESDLQEWRVSYDLDNNSISCSCRKFESFGILCCHCLRLFIHMNVRSVPEQYILKRWTNLARSESLSNVGVSNVVEDVDLSPVQRYNQICPHLIRIAAEACRNPETFTLLCKVSNELNRHLVEFQHNPTSICKVNEFVAKVKEIGSSSDGSSKAKGFKKKEGTRRTKRFKSCLERELPKNKKNDGSKASKVQNSKKHTQDHVQYEVYNVAYNDDRGDSQRQVQYELYNVAYNGDRGDSQSQTQYELYNGTYNGDSQSQVNVDNNDTLNQFGVTSFTRLLMQFSTIGLKAGEMAGGIMQEGIHIPFADSKN