AlfalfaGEDB | MS.gene21623

M. sativa cultivar XinJiangDaYe / MS.gene21623

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF06203.17

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21623.t1	XP_003611074.1	91.3	252	20	2	1	251	1	251	2.00E-123	451.8

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21623.t1	G7KC16	91.3	252	20	2	1	251	1	251	1.5e-123	451.8

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene21623.t1	TR	Others

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21623.t1	MTR_5g010120	91.270	252	20	2	1	251	1	251	1.55e-161	447
MS.gene21623.t1	MTR_8g098725	49.505	202	69	5	65	240	97	291	4.87e-54	177
MS.gene21623.t1	MTR_8g098725	47.573	206	71	5	65	240	97	295	3.24e-52	172
MS.gene21623.t1	MTR_2g068730	54.545	88	37	1	148	232	136	223	2.20e-22	93.2
MS.gene21623.t1	MTR_1g044785	47.917	96	41	2	141	236	107	193	9.22e-20	85.1
MS.gene21623.t1	MTR_2g096080	41.739	115	45	2	129	221	90	204	1.34e-19	85.1
MS.gene21623.t1	MTR_1g073350	51.948	77	37	0	139	215	283	359	7.01e-19	85.5
MS.gene21623.t1	MTR_4g061823	36.913	149	66	4	89	221	58	194	4.18e-17	78.2
MS.gene21623.t1	MTR_4g061910	36.913	149	66	4	89	221	58	194	4.18e-17	78.2
MS.gene21623.t1	MTR_1g008220	48.276	87	38	2	138	220	194	277	7.03e-17	78.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21623.t1	AT5G41380	48.370	184	73	6	58	221	83	264	2.49e-40	141
MS.gene21623.t1	AT1G63820	49.342	152	54	5	117	248	117	265	2.44e-35	128
MS.gene21623.t1	AT3G12890	51.546	97	44	2	154	250	121	214	1.14e-21	90.9
MS.gene21623.t1	AT1G04500	33.484	221	107	8	34	234	146	346	4.62e-20	88.6
MS.gene21623.t1	AT1G04500	33.484	221	107	8	34	234	146	346	4.62e-20	88.6
MS.gene21623.t1	AT1G04500	33.484	221	107	8	34	234	137	337	4.70e-20	88.6
MS.gene21623.t1	AT2G33350	66.667	57	19	0	163	219	301	357	4.88e-18	82.8
MS.gene21623.t1	AT2G33350	66.667	57	19	0	163	219	302	358	5.78e-18	82.8
MS.gene21623.t1	AT2G33350	66.667	57	19	0	163	219	290	346	6.00e-18	82.8
MS.gene21623.t1	AT2G33350	66.667	57	19	0	163	219	310	366	6.23e-18	82.8

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Find 53 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTGAGGAATGTCAAAT+GGG	0.562447	5.2:+3488724	MS.gene21623:CDS
AAATTTCCAAGCTCACTGGT+TGG	0.575257	5.2:-3488676	None:intergenic
AATTCATGCCATTGGAAATT+TGG	0.108434	5.2:-3488859	None:intergenic
ACGTATGCGTGTTCGATTGT+CGG	0.459741	5.2:-3489619	None:intergenic
ACTACCAAAAGTTAATGCAG+AGG	0.669632	5.2:+3488761	MS.gene21623:CDS
ACTGTTTCAATGTAATTGAA+AGG	0.489675	5.2:-3488592	None:intergenic
ACTTCATCCGTTTGAGACCC+AGG	0.512208	5.2:-3489179	None:intergenic
AGAGAAATTTCCAAGCTCAC+TGG	0.521246	5.2:-3488680	None:intergenic
AGCTCCTCTGCATTAACTTT+TGG	0.308354	5.2:-3488765	None:intergenic
AGCTTACCATAACACACAGA+TGG	0.590460	5.2:+3488831	MS.gene21623:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACTGAGGAATGTCAAAT+GGG	+	chr5.2:3488724-3488743	MS.gene21623:CDS	30.0%
AAATAACCTAATTAACACCG+TGG	+	chr5.2:3488953-3488972	MS.gene21623:intron	30.0%
AATTCATGCCATTGGAAATT+TGG	-	chr5.2:3488862-3488881	None:intergenic	30.0%
AGAAAAAGAAAAGAAGGAAG+AGG	-	chr5.2:3488653-3488672	None:intergenic	30.0%
ATGTAATTGAAAGGGTAATG+AGG	-	chr5.2:3488586-3488605	None:intergenic	30.0%
ATTCATGCCATTGGAAATTT+GGG	-	chr5.2:3488861-3488880	None:intergenic	30.0%
GAAATTTCTCTACTTTGGAT+GGG	+	chr5.2:3488692-3488711	MS.gene21623:CDS	30.0%
GATGAAGTAAACTTCAAAGT+AGG	+	chr5.2:3489194-3489213	MS.gene21623:CDS	30.0%
GTTTAATACTTGCATCAATG+TGG	+	chr5.2:3489803-3489822	MS.gene21623:intron	30.0%
TGGAGAAGAAAAAGAAAAGA+AGG	-	chr5.2:3488659-3488678	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	3488505	3489895	3488505	ID=MS.gene21623
chr5.2	mRNA	3488505	3489895	3488505	ID=MS.gene21623.t1;Parent=MS.gene21623
chr5.2	exon	3488505	3488948	3488505	ID=MS.gene21623.t1.exon1;Parent=MS.gene21623.t1
chr5.2	CDS	3488505	3488948	3488505	ID=cds.MS.gene21623.t1;Parent=MS.gene21623.t1
chr5.2	exon	3489167	3489301	3489167	ID=MS.gene21623.t1.exon2;Parent=MS.gene21623.t1
chr5.2	CDS	3489167	3489301	3489167	ID=cds.MS.gene21623.t1;Parent=MS.gene21623.t1
chr5.2	exon	3489597	3489731	3489597	ID=MS.gene21623.t1.exon3;Parent=MS.gene21623.t1
chr5.2	CDS	3489597	3489731	3489597	ID=cds.MS.gene21623.t1;Parent=MS.gene21623.t1
chr5.2	exon	3489854	3489895	3489854	ID=MS.gene21623.t1.exon4;Parent=MS.gene21623.t1
chr5.2	CDS	3489854	3489895	3489854	ID=cds.MS.gene21623.t1;Parent=MS.gene21623.t1

Gene Sequence

>MS.gene21623

ATGTCTTCTGATAGTGATATCTATACTATTGATATTTCTTTCCATAAACATTCAAGCCCAAACAAAGTCTCTTATGATCCTCATTACCCTTTCAATTACATTGAAACAGTTCAAGAGGATTTGAACAATTCTCTTGACTCATTTTCCTCTTCCTTCTTTTCTTTTTCTTCTCCAACCAGTGAGCTTGGAAATTTCTCTACTTTGGATGGGTCTCGTTTGAAAACTGAGGAATGTCAAATGGGTGTAGATTACTCCTACTACCAAAAGTTAATGCAGAGGAGCTATAATTGTAACAATTTTCATGAGAAACCTGATTTTCCTTCTGAAGCTTACCATAACACACAGATGGACTCCCCAAATTTCCAATGGCATGAATTGAGCTCCCCCGAAAATAGTTTCTTCACCGGACAAATAAGAAGGGCGTGCAGTGCAGGAGATTTGCAGGTTAAAATAACCTAATTAACACCGTGGCTTACCATATATATTTTTGAGCATCCACACTTCAGATTAAAAAATGTATATGGTGTCCGATACGTATTAATTTAATCATTTTCATTTTTTAAATGTAGGGTTTGATCTCCGTGTTTATGTCTATTATTTGATAGTAGATGGTTTATTTATTTAATTTTATCTGTATTAAAGGTATGCTGTTTATTGATCAGAACATGGCAACACCTGGGTCTCAAACGGATGAAGTAAACTTCAAAGTAGGGCGTTACAATTTGGAAGAACGAAAAGAAAAAATCTCTAGATATAGAGCCAAGAGAAGCCGGAGGAAGTTCAACAAGATTATTAAGGTATAACACTAATTAACTACTGCGAGTTTGCGTATGCTCCAACATTACCTGAAAGGGGTATATCTGAACTCATTAACATGTTATATACAAAAGAAAGTTGTTCCCTCGGAAGTAAAAAAAAAAATGTTTTGTCAACAAAATATTTATATTTGAACGAAATTTTGTCAAATATATCAACTTTACTAGTAATAATTGAAGGACGAGAATGATGTATTAGTTTATTAAAGATTCCACATATTTTTTGTTTCTAAATATGTGGTTTCTTTTGTTGTTTTTAATTGTGGTACCACAACAGTATGCATGCCGAAAGACACTAGCCGACAATCGAACACGCATACGTGGCAGATTCGCAAGTAACGACAAAACCAACGAGATTCCAAAACCTGTGTGTTCAAAAACAGAAAAATATCATCATGAAGACGAGTTCTGGGTAAGTACGATTTTATGTATTGAATCTTAACATTTACATTATTATGTAATTAATATTAACTAAATTAAACTGTTTAATACTTGCATCAATGTGGTGTTAATATTATTGAGGTTTTTATTTAGGTTGATTTCATTGAAGGGTTAAATGACGAGCTCCTCTATTAA

Protein sequence

>MS.gene21623.t1

MSSDSDIYTIDISFHKHSSPNKVSYDPHYPFNYIETVQEDLNNSLDSFSSSFFSFSSPTSELGNFSTLDGSRLKTEECQMGVDYSYYQKLMQRSYNCNNFHEKPDFPSEAYHNTQMDSPNFQWHELSSPENSFFTGQIRRACSAGDLQNMATPGSQTDEVNFKVGRYNLEERKEKISRYRAKRSRRKFNKIIKYACRKTLADNRTRIRGRFASNDKTNEIPKPVCSKTEKYHHEDEFWVDFIEGLNDELLY