AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21857

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21857.t1 MTR_5g007600 91.892 296 24 0 1 296 1 296 0.0 542
MS.gene21857.t1 MTR_5g007600 85.577 104 15 0 121 224 223 326 1.54e-58 191
MS.gene21857.t1 MTR_1g046680 69.874 239 57 3 1 224 1 239 8.59e-99 291
MS.gene21857.t1 MTR_1g046680 72.000 75 21 0 222 296 135 209 1.60e-33 123
MS.gene21857.t1 MTR_8g013950 41.398 186 98 4 46 224 112 293 4.53e-33 124
MS.gene21857.t1 MTR_8g013950 57.143 49 21 0 248 296 215 263 1.19e-12 67.8
MS.gene21857.t1 MTR_2g094600 44.720 161 66 3 83 220 119 279 2.85e-31 119
MS.gene21857.t1 MTR_2g094600 65.116 43 15 0 247 289 204 246 7.14e-13 68.2
MS.gene21857.t1 MTR_1g094930 54.545 99 45 0 122 220 112 210 9.61e-31 115
MS.gene21857.t1 MTR_1g094930 49.296 71 34 2 220 289 108 177 4.55e-12 65.1
MS.gene21857.t1 MTR_8g076340 70.000 70 21 0 155 224 216 285 3.45e-30 115
MS.gene21857.t1 MTR_8g076340 62.500 40 15 0 257 296 216 255 6.04e-12 65.5
MS.gene21857.t1 MTR_3g109760 38.974 195 90 5 46 224 93 274 3.64e-30 116
MS.gene21857.t1 MTR_3g109760 47.458 59 31 0 238 296 186 244 4.29e-12 66.2
MS.gene21857.t1 MTR_1g094930 54.545 99 45 0 122 220 207 305 3.65e-30 116
MS.gene21857.t1 MTR_1g094930 49.296 71 34 2 220 289 203 272 7.11e-12 65.5
MS.gene21857.t1 MTR_6g032830 73.846 65 17 0 156 220 191 255 6.21e-30 114
MS.gene21857.t1 MTR_6g032830 64.103 39 14 0 258 296 191 229 3.26e-11 62.8
MS.gene21857.t1 MTR_1g006650 71.831 71 20 0 157 227 234 304 9.82e-30 115
MS.gene21857.t1 MTR_1g006650 77.419 31 7 0 259 289 234 264 3.85e-11 63.5
MS.gene21857.t1 MTR_7g085410 75.000 64 16 0 157 220 188 251 2.54e-29 113
MS.gene21857.t1 MTR_7g085410 65.789 38 13 0 259 296 188 225 6.45e-11 62.0
MS.gene21857.t1 MTR_4g058910 62.651 83 30 1 139 220 295 377 2.92e-28 112
MS.gene21857.t1 MTR_4g058910 54.000 50 23 0 247 296 302 351 7.10e-11 62.8
MS.gene21857.t1 MTR_4g071590 42.308 156 60 4 93 224 129 278 3.37e-28 110
MS.gene21857.t1 MTR_4g071590 41.071 112 50 5 191 296 147 248 9.88e-12 64.7
MS.gene21857.t1 MTR_4g471590 42.308 156 60 4 93 224 129 278 3.37e-28 110
MS.gene21857.t1 MTR_4g471590 41.071 112 50 5 191 296 147 248 9.88e-12 64.7
MS.gene21857.t1 MTR_1g116010 68.182 66 21 0 155 220 162 227 3.89e-28 109
MS.gene21857.t1 MTR_1g116010 72.727 33 9 0 257 289 162 194 2.06e-11 63.2
MS.gene21857.t1 MTR_5g021340 66.667 72 24 0 157 228 300 371 5.67e-28 112
MS.gene21857.t1 MTR_3g117380 59.259 81 33 0 148 228 220 300 8.36e-28 110
MS.gene21857.t1 MTR_7g446110 54.839 62 28 0 157 218 187 248 1.77e-17 80.5
MS.gene21857.t1 MTR_7g445980 53.846 65 29 1 155 218 31 95 2.02e-16 73.9
MS.gene21857.t1 MTR_7g445950 51.613 62 30 0 157 218 120 181 1.89e-15 73.6
MS.gene21857.t1 MTR_7g446070 50.000 62 31 0 157 218 132 193 8.63e-15 72.0
MS.gene21857.t1 MTR_5g020230 34.234 111 55 3 92 189 49 154 3.86e-11 62.4
MS.gene21857.t1 MTR_7g112330 57.143 42 18 0 151 192 16 57 8.24e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21857.t1 AT3G24050 40.444 225 99 5 24 220 40 257 1.09e-41 145
MS.gene21857.t1 AT3G24050 54.098 61 21 1 243 296 171 231 2.01e-14 72.4
MS.gene21857.t1 AT4G34680 47.857 140 58 4 96 224 112 247 1.08e-33 124
MS.gene21857.t1 AT4G34680 47.887 71 35 1 222 290 141 211 1.86e-12 66.6
MS.gene21857.t1 AT4G34680 47.857 140 58 4 96 224 112 247 1.08e-33 124
MS.gene21857.t1 AT4G34680 47.887 71 35 1 222 290 141 211 1.86e-12 66.6
MS.gene21857.t1 AT3G60530 47.967 123 56 2 109 224 104 225 1.78e-31 118
MS.gene21857.t1 AT3G60530 46.429 56 30 0 241 296 140 195 7.68e-11 61.6
MS.gene21857.t1 AT1G08010 39.773 176 67 5 78 222 118 285 4.36e-31 118
MS.gene21857.t1 AT1G08010 50.980 51 25 0 246 296 207 257 6.22e-11 62.8
MS.gene21857.t1 AT1G08010 39.773 176 67 5 78 222 118 285 4.36e-31 118
MS.gene21857.t1 AT1G08010 50.980 51 25 0 246 296 207 257 6.22e-11 62.8
MS.gene21857.t1 AT1G08010 39.773 176 67 5 78 222 118 285 4.36e-31 118
MS.gene21857.t1 AT1G08010 50.980 51 25 0 246 296 207 257 6.22e-11 62.8
MS.gene21857.t1 AT4G32890 72.464 69 19 0 156 224 196 264 1.76e-30 117
MS.gene21857.t1 AT4G32890 75.000 32 8 0 258 289 196 227 3.66e-11 63.2
MS.gene21857.t1 AT2G45050 45.578 147 56 4 101 224 101 246 7.79e-30 114
MS.gene21857.t1 AT5G25830 73.529 68 18 0 157 224 219 286 1.63e-29 115
MS.gene21857.t1 AT1G08000 38.542 192 87 6 56 220 94 281 2.03e-29 114
MS.gene21857.t1 AT1G08000 38.542 192 87 6 56 220 94 281 2.03e-29 114
MS.gene21857.t1 AT5G66320 72.059 68 19 0 157 224 249 316 4.27e-29 114
MS.gene21857.t1 AT5G66320 68.421 38 12 0 259 296 249 286 3.04e-11 63.9
MS.gene21857.t1 AT5G66320 72.059 68 19 0 157 224 249 316 4.27e-29 114
MS.gene21857.t1 AT5G66320 68.421 38 12 0 259 296 249 286 3.04e-11 63.9
MS.gene21857.t1 AT3G51080 72.059 68 19 0 157 224 221 288 7.31e-29 112
MS.gene21857.t1 AT4G36240 35.644 202 104 5 23 220 48 227 2.19e-28 109
MS.gene21857.t1 AT3G45170 44.366 142 57 4 86 224 60 182 3.70e-28 108
MS.gene21857.t1 AT3G54810 70.312 64 19 0 157 220 229 292 2.72e-26 106
MS.gene21857.t1 AT3G54810 70.312 64 19 0 157 220 229 292 2.72e-26 106
MS.gene21857.t1 AT2G28340 64.516 62 22 0 159 220 193 254 7.99e-23 96.3
MS.gene21857.t1 AT3G06740 63.889 36 13 0 157 192 41 76 4.07e-11 60.8

Find 80 sgRNAs with CRISPR-Local

Find 136 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTTTGCAGGGCGGTATTC+AGG 0.237442 5.1:-1940245 None:intergenic
ATGTGCTTAGATTGGGGATT+AGG 0.251282 5.1:+1940880 MS.gene21857:CDS
TGTGCTTAGATTGGGGATTA+GGG 0.343205 5.1:+1940881 MS.gene21857:CDS
GGTTTGCTTCGATTGTTCTT+TGG 0.359998 5.1:-1940402 None:intergenic
GTTGTTTCCACGCGAATTGA+TGG 0.373020 5.1:-1940058 None:intergenic
CACATCACATGTGCTTAGAT+TGG 0.390703 5.1:+1940872 MS.gene21857:CDS
ATGCATGTGGGGTTAGGTAT+AGG 0.393134 5.1:+1940207 MS.gene21857:CDS
CGAAATATCTGGTTGAATAC+AGG 0.393474 5.1:-1939865 None:intergenic
CCACGCGAATTGATGGTTCC+AGG 0.401734 5.1:-1940051 None:intergenic
AGCTGGAAATGCATCTTTGT+TGG 0.402382 5.1:-1939820 None:intergenic
GCTGTTTCCACGTGAATTGA+CGG 0.414591 5.1:-1940364 None:intergenic
TTTGTAATGCATGTGGGGTT+AGG 0.415099 5.1:+1940201 MS.gene21857:CDS
TCTTTGGATGCTTTGTTACT+CGG 0.415607 5.1:-1940386 None:intergenic
ACATCACATGTGCTTAGATT+GGG 0.417527 5.1:+1940873 MS.gene21857:CDS
TCATCAAATGTTTCAACAGC+TGG 0.422269 5.1:-1939837 None:intergenic
GAGCTGATAATACTCCTCTA+TGG 0.452228 5.1:+1940150 MS.gene21857:CDS
CTATGGCGTGCAGGTCCTGC+TGG 0.452250 5.1:+1940167 MS.gene21857:CDS
CTTGTGCTGTTCTCAACAAT+TGG 0.453321 5.1:-1939906 None:intergenic
TAATGCTGTTGGAACTGTTA+TGG 0.453732 5.1:-1939941 None:intergenic
GACTTGCTCCGTGCACGGAC+AGG 0.469909 5.1:-1939996 None:intergenic
TTGATTCTGTGGATGACCTA+TGG 0.476926 5.1:+1939193 MS.gene21857:CDS
TTTGTAATGCATGTGGGGTC+AGG 0.477301 5.1:+1940507 MS.gene21857:CDS
AACAGCATTACACTCTTAAG+TGG 0.478526 5.1:+1939954 MS.gene21857:CDS
GCCTTGGCATTTCTGACACA+TGG 0.480082 5.1:+1940339 MS.gene21857:CDS
ACATTTCCTCCCAATGGTTG+AGG 0.482486 5.1:-1940423 None:intergenic
CGAAGCAAACCTCAACCATT+GGG 0.485894 5.1:+1940414 MS.gene21857:CDS
GCGAAACAAACCTCAACCAT+TGG 0.490923 5.1:+1940107 MS.gene21857:CDS
TTAAACTTACAGAAAATGAA+TGG 0.491207 5.1:-1939296 None:intergenic
GCCTCGGCATTTCTGATACC+TGG 0.496075 5.1:+1940033 MS.gene21857:CDS
ACATTGCCTCCCAATGGTTG+AGG 0.497491 5.1:-1940117 None:intergenic
TCGAAGCAAACCTCAACCAT+TGG 0.499266 5.1:+1940413 MS.gene21857:CDS
AGCATTTCCTTCATTTGACC+TGG 0.504334 5.1:+1939263 MS.gene21857:CDS
CGAAACAAACCTCAACCATT+GGG 0.506442 5.1:+1940108 MS.gene21857:CDS
ACTTACAGAAAATGAATGGT+TGG 0.511740 5.1:-1939292 None:intergenic
GATGGAGGCTCTTGATTCTG+TGG 0.514579 5.1:+1939182 None:intergenic
GTTCCAGGAGTTTGCAGTAG+AGG 0.515595 5.1:+1939782 MS.gene21857:intron
AATACTCCGCAATGGCGTGC+AGG 0.516576 5.1:+1940464 MS.gene21857:CDS
TGAAGTTCCCTGTCCGTGCA+CGG 0.541394 5.1:+1939988 MS.gene21857:CDS
TGGAGTTTCTTATCTGACAT+AGG 0.541869 5.1:+1939213 MS.gene21857:CDS
TTGCAGTAGAGGATCTTGAA+TGG 0.544677 5.1:+1939793 MS.gene21857:CDS
AATACTCCTCTATGGCGTGC+AGG 0.546436 5.1:+1940158 MS.gene21857:CDS
TGTGGGGTTAGGTATAGGTC+TGG 0.555845 5.1:+1940212 MS.gene21857:CDS
ATGAATGGTTGGTATCATCC+AGG 0.557618 5.1:-1939281 None:intergenic
TATTTCCACTACTTTCCTGT+GGG 0.558023 5.1:-1940300 None:intergenic
TGCGAGACTTGCTCCGTGCA+CGG 0.562316 5.1:-1940001 None:intergenic
ACTTGCTCCGTGCACGGACA+GGG 0.574860 5.1:-1939995 None:intergenic
AACACTTTGTAATGCATGTG+GGG 0.575624 5.1:+1940196 MS.gene21857:CDS
GTATCATCCAGGTCAAATGA+AGG 0.576270 5.1:-1939270 None:intergenic
GCAGGACCTGCACGCCATAG+AGG 0.577006 5.1:-1940164 None:intergenic
AAAACACTTTGTAATGCATG+TGG 0.577073 5.1:+1940194 MS.gene21857:CDS
AAAACACTTTGTAATGCATG+TGG 0.577073 5.1:+1940500 MS.gene21857:CDS
ACTTAAGAGTGTAATGCTGT+TGG 0.577340 5.1:-1939952 None:intergenic
ATGATGACATTTCCTCCCAA+TGG 0.580868 5.1:-1940429 None:intergenic
CATCACATGTGCTTAGATTG+GGG 0.585297 5.1:+1940874 MS.gene21857:CDS
CAATGGCGTGCAGGTCCCGA+TGG 0.590689 5.1:+1940473 MS.gene21857:CDS
ATTCGTAGTAAGCCTCGCCT+TGG 0.591249 5.1:+1940323 MS.gene21857:CDS
TTATTTCCACTACTTTCCTG+TGG 0.598127 5.1:-1940301 None:intergenic
AAACACTTTGTAATGCATGT+GGG 0.605185 5.1:+1940195 MS.gene21857:CDS
TAATTCCCACAGGAAAGTAG+TGG 0.608211 5.1:+1940295 MS.gene21857:CDS
TCCATGTGTCAGAAATGCCA+AGG 0.617962 5.1:-1940340 None:intergenic
AACACTTTGTAATGCATGTG+GGG 0.618425 5.1:+1940502 MS.gene21857:CDS
AAACACTTTGTAATGCATGT+GGG 0.620936 5.1:+1940501 MS.gene21857:CDS
GGGAGGAAATGTCATCATTG+TGG 0.622937 5.1:+1940434 MS.gene21857:CDS
GGGAGGCAATGTCATCATTG+TGG 0.625233 5.1:+1940128 MS.gene21857:CDS
ATGTGCATTCTAATTCCCAC+AGG 0.631252 5.1:+1940285 MS.gene21857:CDS
GGCGGTATTCAGGTACAAGT+CGG 0.632650 5.1:-1940235 None:intergenic
TTTCTTATCTGACATAGGCG+AGG 0.637448 5.1:+1939218 MS.gene21857:CDS
AACAAACCTCAACCATTGGG+AGG 0.638620 5.1:+1940111 MS.gene21857:CDS
CCTGGAACCATCAATTCGCG+TGG 0.640359 5.1:+1940051 MS.gene21857:CDS
TCCAGGTATCAGAAATGCCG+AGG 0.643154 5.1:-1940034 None:intergenic
TGTCAGATAAGAAACTCCAT+AGG 0.643894 5.1:-1939209 None:intergenic
CATGGAACCGTCAATTCACG+TGG 0.644051 5.1:+1940357 MS.gene21857:CDS
TCTCGCAGTAAGCCTCGCCT+CGG 0.646601 5.1:+1940017 MS.gene21857:CDS
TCGGGACCTGCACGCCATTG+CGG 0.648022 5.1:-1940470 None:intergenic
ATGATGACATTGCCTCCCAA+TGG 0.653586 5.1:-1940123 None:intergenic
GTATCAGAAATGCCGAGGCG+AGG 0.653593 5.1:-1940029 None:intergenic
GTGTCAGAAATGCCAAGGCG+AGG 0.655134 5.1:-1940335 None:intergenic
AGCAAACCTCAACCATTGGG+AGG 0.655985 5.1:+1940417 MS.gene21857:CDS
GAGCTGATAATACTCCGCAA+TGG 0.677467 5.1:+1940456 MS.gene21857:CDS
AAGTTGGGCTCTTTGCAGGG+CGG 0.700990 5.1:-1940253 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGATTTTCTTTTTTACTT+TGG + chr5.1:1940721-1940740 MS.gene21857:intron 15.0%
!! AATCATTTCAATGTCAAATT+TGG + chr5.1:1940829-1940848 MS.gene21857:intron 20.0%
!! TTAAACTTACAGAAAATGAA+TGG - chr5.1:1939299-1939318 None:intergenic 20.0%
!! TTTGACATTGAAATGATTTA+AGG - chr5.1:1940827-1940846 None:intergenic 20.0%
!!! AAATTTGGTTTTTGCATTTT+AGG + chr5.1:1940844-1940863 MS.gene21857:intron 20.0%
!!! AAGAGAAAAGTATTATGATT+TGG + chr5.1:1939411-1939430 MS.gene21857:intron 20.0%
!!! AAGTTAGTTTGCTATAAATT+AGG + chr5.1:1940696-1940715 MS.gene21857:intron 20.0%
!!! TACTTTCTAAAATTCTACTT+TGG - chr5.1:1939478-1939497 None:intergenic 20.0%
AAAACACTTTGTAATGCATG+TGG + chr5.1:1940194-1940213 MS.gene21857:CDS 30.0%
AAACACTTTGTAATGCATGT+GGG + chr5.1:1940195-1940214 MS.gene21857:CDS 30.0%
ACATACTCGATAATTTCTCA+CGG - chr5.1:1939344-1939363 None:intergenic 30.0%
ACTTACAGAAAATGAATGGT+TGG - chr5.1:1939295-1939314 None:intergenic 30.0%
AGGAAGACAAGTAAAAATCT+AGG - chr5.1:1939503-1939522 None:intergenic 30.0%
ATAGCAAACTAACTTCTACA+AGG - chr5.1:1940691-1940710 None:intergenic 30.0%
TATTCAAACTGAGATAGATG+TGG - chr5.1:1939650-1939669 None:intergenic 30.0%
TTTAACCTTTCCAAAATGCT+TGG + chr5.1:1939314-1939333 MS.gene21857:intron 30.0%
! AATGAAGGAAATGCTTTTGT+AGG - chr5.1:1939258-1939277 None:intergenic 30.0%
! TCCTTTTTGCATCAATGTAT+TGG + chr5.1:1939519-1939538 MS.gene21857:intron 30.0%
!! CATGCATTACAAAGTGTTTT+TGG - chr5.1:1940194-1940213 None:intergenic 30.0%
!! TTTTGATTCTGCGAAATATC+TGG - chr5.1:1939879-1939898 None:intergenic 30.0%
!!! CAGTGAAAGTTTTGAAGTTA+GGG + chr5.1:1940798-1940817 MS.gene21857:intron 30.0%
!!! TATCTAGTTTTGACTTGTTG+AGG + chr5.1:1939377-1939396 MS.gene21857:intron 30.0%
AAAAAGTTGGGCTATTTGCA+GGG - chr5.1:1940565-1940584 None:intergenic 35.0%
AACACTTTGTAATGCATGTG+GGG + chr5.1:1940196-1940215 MS.gene21857:CDS 35.0%
AACAGCATTACACTCTTAAG+TGG + chr5.1:1939954-1939973 MS.gene21857:CDS 35.0%
AAGCAGAAGAGAATGAGAAT+GGG + chr5.1:1940632-1940651 MS.gene21857:intron 35.0%
AAGTGGTAGCTAGTGAAATA+TGG + chr5.1:1940665-1940684 MS.gene21857:intron 35.0%
ACATCACATGTGCTTAGATT+GGG + chr5.1:1940873-1940892 MS.gene21857:CDS 35.0%
CGAAATATCTGGTTGAATAC+AGG - chr5.1:1939868-1939887 None:intergenic 35.0%
GAATGGGATGAAATGAAAAG+TGG + chr5.1:1940648-1940667 MS.gene21857:intron 35.0%
GCCAATACATTGATGCAAAA+AGG - chr5.1:1939523-1939542 None:intergenic 35.0%
TAATGCTGTTGGAACTGTTA+TGG - chr5.1:1939944-1939963 None:intergenic 35.0%
TATTTCCACTACTTTCCTGT+GGG - chr5.1:1940303-1940322 None:intergenic 35.0%
TCATCAAATGTTTCAACAGC+TGG - chr5.1:1939840-1939859 None:intergenic 35.0%
TGGAGTTTCTTATCTGACAT+AGG + chr5.1:1939213-1939232 MS.gene21857:CDS 35.0%
TGTCAGATAAGAAACTCCAT+AGG - chr5.1:1939212-1939231 None:intergenic 35.0%
TTATTTCCACTACTTTCCTG+TGG - chr5.1:1940304-1940323 None:intergenic 35.0%
! ACAGGAAAGTAGTGGAAATA+AGG + chr5.1:1940303-1940322 MS.gene21857:CDS 35.0%
! ACTTAAGAGTGTAATGCTGT+TGG - chr5.1:1939955-1939974 None:intergenic 35.0%
! GACTTGTGATCACTGTTTTA+AGG + chr5.1:1939608-1939627 MS.gene21857:intron 35.0%
! TCTTTGGATGCTTTGTTACT+CGG - chr5.1:1940389-1940408 None:intergenic 35.0%
!!! ACAAAGTGTTTTTGGACCAT+CGG - chr5.1:1940492-1940511 None:intergenic 35.0%
!!! GCAGTGAAAGTTTTGAAGTT+AGG + chr5.1:1940797-1940816 MS.gene21857:intron 35.0%
!!! TGAAAGTTTTGAAGTTAGGG+AGG + chr5.1:1940801-1940820 MS.gene21857:intron 35.0%
!!! TTTAGTCCTTTTTTCGCTGT+CGG + chr5.1:1940761-1940780 MS.gene21857:intron 35.0%
AAAAAGTTGGGCTCTTTGCA+GGG - chr5.1:1940259-1940278 None:intergenic 40.0%
AAACATCCGACAGCGAAAAA+AGG - chr5.1:1940770-1940789 None:intergenic 40.0%
ACCTTTCCAAAATGCTTGGA+AGG + chr5.1:1939318-1939337 MS.gene21857:intron 40.0%
AGCATTTCCTTCATTTGACC+TGG + chr5.1:1939263-1939282 MS.gene21857:CDS 40.0%
AGCTGGAAATGCATCTTTGT+TGG - chr5.1:1939823-1939842 None:intergenic 40.0%
ATGATGACATTTCCTCCCAA+TGG - chr5.1:1940432-1940451 None:intergenic 40.0%
ATGTGCATTCTAATTCCCAC+AGG + chr5.1:1940285-1940304 MS.gene21857:CDS 40.0%
ATGTGCTTAGATTGGGGATT+AGG + chr5.1:1940880-1940899 MS.gene21857:CDS 40.0%
CAAAAAGTTGGGCTATTTGC+AGG - chr5.1:1940566-1940585 None:intergenic 40.0%
CACATCACATGTGCTTAGAT+TGG + chr5.1:1940872-1940891 MS.gene21857:CDS 40.0%
CATCACATGTGCTTAGATTG+GGG + chr5.1:1940874-1940893 MS.gene21857:CDS 40.0%
CGAAACAAACCTCAACCATT+GGG + chr5.1:1940108-1940127 MS.gene21857:CDS 40.0%
GAAGCAGAAGAGAATGAGAA+TGG + chr5.1:1940631-1940650 MS.gene21857:intron 40.0%
GAGCTGATAATACTCCTCTA+TGG + chr5.1:1940150-1940169 MS.gene21857:CDS 40.0%
GCACATTGCTGCAAAAAGTT+GGG - chr5.1:1940271-1940290 None:intergenic 40.0%
GTATCATCCAGGTCAAATGA+AGG - chr5.1:1939273-1939292 None:intergenic 40.0%
GTATTCACACTGAAAACTCC+TGG - chr5.1:1939722-1939741 None:intergenic 40.0%
TAATTCCCACAGGAAAGTAG+TGG + chr5.1:1940295-1940314 MS.gene21857:CDS 40.0%
TCCTGGAACATGAAAACAAG+CGG - chr5.1:1939705-1939724 None:intergenic 40.0%
TGCACATTGCTGCAAAAAGT+TGG - chr5.1:1940272-1940291 None:intergenic 40.0%
TGTGCTTAGATTGGGGATTA+GGG + chr5.1:1940881-1940900 MS.gene21857:CDS 40.0%
TTGATTCTGTGGATGACCTA+TGG + chr5.1:1939193-1939212 MS.gene21857:CDS 40.0%
TTGCAGTAGAGGATCTTGAA+TGG + chr5.1:1939793-1939812 MS.gene21857:CDS 40.0%
TTTCTTATCTGACATAGGCG+AGG + chr5.1:1939218-1939237 MS.gene21857:CDS 40.0%
TTTGTAATGCATGTGGGGTT+AGG + chr5.1:1940201-1940220 MS.gene21857:CDS 40.0%
! ATGAATGGTTGGTATCATCC+AGG - chr5.1:1939284-1939303 None:intergenic 40.0%
! CTTGTGCTGTTCTCAACAAT+TGG - chr5.1:1939909-1939928 None:intergenic 40.0%
! GGTTTGCTTCGATTGTTCTT+TGG - chr5.1:1940405-1940424 None:intergenic 40.0%
!!! CAAAGTGTTTTTGGACCATC+GGG - chr5.1:1940491-1940510 None:intergenic 40.0%
!!! GTTTTAAGGTTTCCGTGTTG+TGG + chr5.1:1939622-1939641 MS.gene21857:intron 40.0%
AACAAACCTCAACCATTGGG+AGG + chr5.1:1940111-1940130 MS.gene21857:CDS 45.0%
ACATTTCCTCCCAATGGTTG+AGG - chr5.1:1940426-1940445 None:intergenic 45.0%
ATAGATGTGGCTCCACAACA+CGG - chr5.1:1939637-1939656 None:intergenic 45.0%
ATGATGACATTGCCTCCCAA+TGG - chr5.1:1940126-1940145 None:intergenic 45.0%
ATGCATGTGGGGTTAGGTAT+AGG + chr5.1:1940207-1940226 MS.gene21857:CDS 45.0%
CAAAAAGTTGGGCTCTTTGC+AGG - chr5.1:1940260-1940279 None:intergenic 45.0%
CGAAGCAAACCTCAACCATT+GGG + chr5.1:1940414-1940433 MS.gene21857:CDS 45.0%
GAGCTGATAATACTCCGCAA+TGG + chr5.1:1940456-1940475 MS.gene21857:CDS 45.0%
GCAAAGAGAGAATCTGTCGT+TGG - chr5.1:1939573-1939592 None:intergenic 45.0%
GCGAAACAAACCTCAACCAT+TGG + chr5.1:1940107-1940126 MS.gene21857:CDS 45.0%
GGGAGGAAATGTCATCATTG+TGG + chr5.1:1940434-1940453 MS.gene21857:CDS 45.0%
TAATGCCCACAGGAAAGTAG+TGG + chr5.1:1940601-1940620 MS.gene21857:intron 45.0%
TCCATGTGTCAGAAATGCCA+AGG - chr5.1:1940343-1940362 None:intergenic 45.0%
TCGAAGCAAACCTCAACCAT+TGG + chr5.1:1940413-1940432 MS.gene21857:CDS 45.0%
TTTGTAATGCATGTGGGGTC+AGG + chr5.1:1940507-1940526 MS.gene21857:CDS 45.0%
! GCTGTTTCCACGTGAATTGA+CGG - chr5.1:1940367-1940386 None:intergenic 45.0%
! GTTGTTTCCACGCGAATTGA+TGG - chr5.1:1940061-1940080 None:intergenic 45.0%
!! CGCTGTCGGATGTTTTTGAT+GGG + chr5.1:1940775-1940794 MS.gene21857:intron 45.0%
!! GCCTTCCAAGCATTTTGGAA+AGG - chr5.1:1939322-1939341 None:intergenic 45.0%
!! TCGCTGTCGGATGTTTTTGA+TGG + chr5.1:1940774-1940793 MS.gene21857:intron 45.0%
!! TCTGAGATTTTCAGTGCCTG+CGG + chr5.1:1939545-1939564 MS.gene21857:intron 45.0%
!!! CAAAGTGTTTTTGGACCAGC+AGG - chr5.1:1940185-1940204 None:intergenic 45.0%
AAGTTGGGCTATTTGCAGGG+CGG - chr5.1:1940562-1940581 None:intergenic 50.0%
AATACTCCTCTATGGCGTGC+AGG + chr5.1:1940158-1940177 MS.gene21857:CDS 50.0%
ACATTGCCTCCCAATGGTTG+AGG - chr5.1:1940120-1940139 None:intergenic 50.0%
ACGTGCATTCTAATGCCCAC+AGG + chr5.1:1940591-1940610 MS.gene21857:intron 50.0%
AGCAAACCTCAACCATTGGG+AGG + chr5.1:1940417-1940436 MS.gene21857:CDS 50.0%
ATGCATGTGGGGTCAGGTAT+AGG + chr5.1:1940513-1940532 MS.gene21857:intron 50.0%
ATTCGTAGTAAGCCTCGCCT+TGG + chr5.1:1940323-1940342 MS.gene21857:CDS 50.0%
CATGGAACCGTCAATTCACG+TGG + chr5.1:1940357-1940376 MS.gene21857:CDS 50.0%
CTATTTGCAGGGCGGTATTC+AGG - chr5.1:1940554-1940573 None:intergenic 50.0%
GATCCTCTACTGCAAACTCC+TGG - chr5.1:1939788-1939807 None:intergenic 50.0%
GCACGTCGCTGCAAAAAGTT+GGG - chr5.1:1940577-1940596 None:intergenic 50.0%
GCCTTGGCATTTCTGACACA+TGG + chr5.1:1940339-1940358 MS.gene21857:CDS 50.0%
GGCGGTATTCAGGTACAAGT+CGG - chr5.1:1940238-1940257 None:intergenic 50.0%
GGGAGGCAATGTCATCATTG+TGG + chr5.1:1940128-1940147 MS.gene21857:CDS 50.0%
GTTCCAGGAGTTTGCAGTAG+AGG + chr5.1:1939782-1939801 MS.gene21857:intron 50.0%
TCCAGGTATCAGAAATGCCG+AGG - chr5.1:1940037-1940056 None:intergenic 50.0%
TGCACGTCGCTGCAAAAAGT+TGG - chr5.1:1940578-1940597 None:intergenic 50.0%
TGTGGGGTTAGGTATAGGTC+TGG + chr5.1:1940212-1940231 MS.gene21857:CDS 50.0%
! TCACGGCCTTCCAAGCATTT+TGG - chr5.1:1939327-1939346 None:intergenic 50.0%
!! GCCGCTTGTTTTCATGTTCC+AGG + chr5.1:1939701-1939720 MS.gene21857:intron 50.0%
AAGTTGGGCTCTTTGCAGGG+CGG - chr5.1:1940256-1940275 None:intergenic 55.0%
AATACTCCGCAATGGCGTGC+AGG + chr5.1:1940464-1940483 MS.gene21857:CDS 55.0%
CCTGGAACCATCAATTCGCG+TGG + chr5.1:1940051-1940070 MS.gene21857:CDS 55.0%
CTCTTTGCAGGGCGGTATTC+AGG - chr5.1:1940248-1940267 None:intergenic 55.0%
GCCTCGGCATTTCTGATACC+TGG + chr5.1:1940033-1940052 MS.gene21857:CDS 55.0%
GTATCAGAAATGCCGAGGCG+AGG - chr5.1:1940032-1940051 None:intergenic 55.0%
GTGTCAGAAATGCCAAGGCG+AGG - chr5.1:1940338-1940357 None:intergenic 55.0%
TGAAGTTCCCTGTCCGTGCA+CGG + chr5.1:1939988-1940007 MS.gene21857:CDS 55.0%
TGTGGGGTCAGGTATAGGTC+TGG + chr5.1:1940518-1940537 MS.gene21857:intron 55.0%
! CCACGCGAATTGATGGTTCC+AGG - chr5.1:1940054-1940073 None:intergenic 55.0%
ACTTGCTCCGTGCACGGACA+GGG - chr5.1:1939998-1940017 None:intergenic 60.0%
GAATCTGTCGTTGGCACCGC+AGG - chr5.1:1939564-1939583 None:intergenic 60.0%
TCTCGCAGTAAGCCTCGCCT+CGG + chr5.1:1940017-1940036 MS.gene21857:CDS 60.0%
! TGCGAGACTTGCTCCGTGCA+CGG - chr5.1:1940004-1940023 None:intergenic 60.0%
CAATGGCGTGCAGGTCCCGA+TGG + chr5.1:1940473-1940492 MS.gene21857:CDS 65.0%
CTATGGCGTGCAGGTCCTGC+TGG + chr5.1:1940167-1940186 MS.gene21857:CDS 65.0%
GCAGGACCTGCACGCCATAG+AGG - chr5.1:1940167-1940186 None:intergenic 65.0%
TCGGGACCTGCACGCCATTG+CGG - chr5.1:1940473-1940492 None:intergenic 65.0%
! GACTTGCTCCGTGCACGGAC+AGG - chr5.1:1939999-1940018 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 1939183 1940923 1939183 ID=MS.gene21857
chr5.1 mRNA 1939183 1940923 1939183 ID=MS.gene21857.t1;Parent=MS.gene21857
chr5.1 exon 1939183 1939308 1939183 ID=MS.gene21857.t1.exon1;Parent=MS.gene21857.t1
chr5.1 CDS 1939183 1939308 1939183 ID=cds.MS.gene21857.t1;Parent=MS.gene21857.t1
chr5.1 exon 1939789 1940528 1939789 ID=MS.gene21857.t1.exon2;Parent=MS.gene21857.t1
chr5.1 CDS 1939789 1940528 1939789 ID=cds.MS.gene21857.t1;Parent=MS.gene21857.t1
chr5.1 exon 1940866 1940923 1940866 ID=MS.gene21857.t1.exon3;Parent=MS.gene21857.t1
chr5.1 CDS 1940866 1940923 1940866 ID=cds.MS.gene21857.t1;Parent=MS.gene21857.t1
Gene Sequence

>MS.gene21857

ATGGAGGCTCTTGATTCTGTGGATGACCTATGGAGTTTCTTATCTGACATAGGCGAGGATGATTATGACAAACCTACAAAAGCATTTCCTTCATTTGACCTGGATGATACCAACCATTCATTTTCTGTAAGTTTAACCTTTCCAAAATGCTTGGAAGGCCGTGAGAAATTATCGAGTATGTTGTTTGATTTTTTTATCTAGTTTTGACTTGTTGAGGTGTGATCATGAAAGAGAAAAGTATTATGATTTGGTTTGAATCAATTAGAATTGTGATATATTATATTGTTTTTGTCCAAAGTAGAATTTTAGAAAGTACTCCTAGATTTTTACTTGTCTTCCTTTTTGCATCAATGTATTGGCAATCTGAGATTTTCAGTGCCTGCGGTGCCAACGACAGATTCTCTCTTTGCATCAAAGTTTTAATTGACTTGTGATCACTGTTTTAAGGTTTCCGTGTTGTGGAGCCACATCTATCTCAGTTTGAATACTAATGTCTTGATCGAAAGTTAATGTGATGTGCCGCTTGTTTTCATGTTCCAGGAGTTTTCAGTGTGAATACTAATGTCTTGATCAAAAACTAATGTGCCGCTTGTTTTCATGTTCCAGGAGTTTGCAGTAGAGGATCTTGAATGGCTATCCAACAAAGATGCATTTCCAGCTGTTGAAACATTTGATGACTTTTCCTGTATTCAACCAGATATTTCGCAGAATCAAAAGATAGCACCAATTGTTGAGAACAGCACAAGTAGTAGCAACAGCCATAACAGTTCCAACAGCATTACACTCTTAAGTGGCTACAATCACGTGAAGTTCCCTGTCCGTGCACGGAGCAAGTCTCGCAGTAAGCCTCGCCTCGGCATTTCTGATACCTGGAACCATCAATTCGCGTGGAAACAACTGAGTAACAAAACTTCTAAAGAACAAGCGAAACAAACCTCAACCATTGGGAGGCAATGTCATCATTGTGGAGCTGATAATACTCCTCTATGGCGTGCAGGTCCTGCTGGTCCAAAAACACTTTGTAATGCATGTGGGGTTAGGTATAGGTCTGGCCGACTTGTACCTGAATACCGCCCTGCAAAGAGCCCAACTTTTTGCAGCAATGTGCATTCTAATTCCCACAGGAAAGTAGTGGAAATAATTCGTAGTAAGCCTCGCCTTGGCATTTCTGACACATGGAACCGTCAATTCACGTGGAAACAGCCGAGTAACAAAGCATCCAAAGAACAATCGAAGCAAACCTCAACCATTGGGAGGAAATGTCATCATTGTGGAGCTGATAATACTCCGCAATGGCGTGCAGGTCCCGATGGTCCAAAAACACTTTGTAATGCATGTGGGGTCAGGTATAGGTCTGGCCGACTTGTACCTGAATACCGCCCTGCAAATAGCCCAACTTTTTGCAGCGACGTGCATTCTAATGCCCACAGGAAAGTAGTGGAAATAAGGAAGCAGAAGAGAATGAGAATGGGATGAAATGAAAAGTGGTAGCTAGTGAAATATGGCCTTGTAGAAGTTAGTTTGCTATAAATTAGGATTTTGATTTTCTTTTTTACTTTGGTCTATTCTAATTGAAATTTTAGTCCTTTTTTCGCTGTCGGATGTTTTTGATGGGCAGTGAAAGTTTTGAAGTTAGGGAGGCCTTAAATCATTTCAATGTCAAATTTGGTTTTTGCATTTTAGGTTGCTCACATCACATGTGCTTAGATTGGGGATTAGGGAGAAGATGAGTTGCAAGTAG
Protein sequence

>MS.gene21857.t1

MEALDSVDDLWSFLSDIGEDDYDKPTKAFPSFDLDDTNHSFSEFAVEDLEWLSNKDAFPAVETFDDFSCIQPDISQNQKIAPIVENSTSSSNSHNSSNSITLLSGYNHVKFPVRARSKSRSKPRLGISDTWNHQFAWKQLSNKTSKEQAKQTSTIGRQCHHCGADNTPLWRAGPAGPKTLCNACGVRYRSGRLVPEYRPAKSPTFCSNVHSNSHRKVVEIIRSKPRLGISDTWNRQFTWKQPSNKASKEQSKQTSTIGRKCHHCGADNTPQWRAGPDGPKTLCNACGVRLLTSHVLRLGIREKMSCK