AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21954

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21954.t1 MTR_5g009080 99.376 481 3 0 1 481 1 481 0.0 991
MS.gene21954.t1 MTR_4g076140 75.574 479 109 6 4 481 1 472 0.0 741
MS.gene21954.t1 MTR_3g022830 54.362 447 192 5 34 480 4 438 4.10e-170 486
MS.gene21954.t1 MTR_7g027190 34.658 453 209 7 45 478 289 673 7.89e-83 270
MS.gene21954.t1 MTR_1g106590 89.831 118 12 0 50 167 124 241 1.79e-67 220
MS.gene21954.t1 MTR_1g106590 90.722 97 9 0 286 382 244 340 5.76e-39 144
MS.gene21954.t1 MTR_5g097480 29.861 432 236 5 53 478 174 544 2.44e-63 215
MS.gene21954.t1 MTR_8g442410 30.455 440 236 10 49 479 148 526 6.47e-60 206
MS.gene21954.t1 MTR_1g029420 28.837 430 239 7 55 478 224 592 2.90e-54 191
MS.gene21954.t1 MTR_3g089055 28.140 430 242 7 55 478 197 565 1.80e-52 186
MS.gene21954.t1 MTR_3g089055 28.140 430 242 7 55 478 202 570 2.02e-52 186
MS.gene21954.t1 MTR_4g064160 28.241 432 236 7 56 477 316 683 1.89e-47 174
MS.gene21954.t1 MTR_4g064120 25.959 443 260 6 42 475 240 623 2.24e-46 171
MS.gene21954.t1 MTR_4g064200 26.852 432 246 7 53 477 281 649 4.60e-46 170
MS.gene21954.t1 MTR_4g104020 27.107 439 251 13 50 479 143 521 4.89e-46 168
MS.gene21954.t1 MTR_4g064150 27.189 434 248 7 53 477 358 732 5.17e-46 171
MS.gene21954.t1 MTR_3g022580 41.872 203 77 4 279 481 25 186 3.26e-45 156
MS.gene21954.t1 MTR_4g064180 26.185 443 259 7 42 475 240 623 6.41e-43 161
MS.gene21954.t1 MTR_2g097310 26.984 441 256 10 42 477 258 637 7.90e-43 160
MS.gene21954.t1 MTR_4g133660 26.887 424 252 10 56 477 175 542 9.73e-43 159
MS.gene21954.t1 MTR_1g069725 28.899 436 236 11 48 480 101 465 8.07e-42 155
MS.gene21954.t1 MTR_2g097390 26.244 442 260 10 41 477 260 640 9.53e-42 157
MS.gene21954.t1 MTR_7g062120 26.573 429 249 7 56 478 372 740 1.42e-41 158
MS.gene21954.t1 MTR_2g097350 26.304 441 259 10 42 477 260 639 9.14e-40 152
MS.gene21954.t1 MTR_7g074650 41.146 192 108 2 60 249 442 630 2.52e-39 152
MS.gene21954.t1 MTR_7g074650 38.255 149 87 3 333 478 646 792 2.97e-23 103
MS.gene21954.t1 MTR_4g077760 26.147 436 242 9 53 478 190 555 4.46e-38 146
MS.gene21954.t1 MTR_2g034280 27.056 462 256 11 33 478 179 575 9.09e-38 145
MS.gene21954.t1 MTR_4g102790 33.023 215 140 1 49 259 285 499 4.26e-37 145
MS.gene21954.t1 MTR_4g102790 32.692 156 98 5 328 477 507 661 2.84e-19 91.3
MS.gene21954.t1 MTR_3g065980 34.685 222 131 3 44 260 160 372 3.03e-36 141
MS.gene21954.t1 MTR_3g065980 37.576 165 90 3 327 478 376 540 6.98e-30 122
MS.gene21954.t1 MTR_2g097463 27.400 427 249 10 53 477 287 654 2.45e-35 139
MS.gene21954.t1 MTR_2g097380 24.700 417 223 9 64 477 232 560 4.76e-35 138
MS.gene21954.t1 MTR_2g089100 24.943 441 274 9 50 477 46 442 6.84e-34 133
MS.gene21954.t1 MTR_2g082090 35.238 210 129 2 53 256 209 417 1.67e-31 127
MS.gene21954.t1 MTR_2g082090 41.611 149 86 1 329 477 431 578 3.14e-29 121
MS.gene21954.t1 MTR_3g022005 54.082 98 44 1 384 481 32 128 2.44e-31 117
MS.gene21954.t1 MTR_5g094450 28.972 214 144 3 53 258 158 371 5.31e-29 120
MS.gene21954.t1 MTR_5g094450 36.879 141 88 1 338 478 393 532 6.79e-24 105
MS.gene21954.t1 MTR_3g027430 48.305 118 40 1 139 256 184 280 1.58e-28 115
MS.gene21954.t1 MTR_2g034260 24.675 462 265 12 33 478 190 584 3.15e-28 118
MS.gene21954.t1 MTR_3g056110 27.304 293 188 7 53 329 172 455 3.03e-26 112
MS.gene21954.t1 MTR_3g056110 36.170 141 89 1 338 478 403 542 3.16e-25 108
MS.gene21954.t1 MTR_2g097410 29.048 210 142 2 56 258 371 580 3.80e-26 112
MS.gene21954.t1 MTR_2g097410 28.188 149 107 0 329 477 592 740 2.47e-18 88.6
MS.gene21954.t1 MTR_2g026250 29.817 218 142 4 57 267 229 442 6.01e-26 111
MS.gene21954.t1 MTR_2g026250 34.507 142 92 1 338 479 457 597 7.39e-21 96.3
MS.gene21954.t1 MTR_7g109580 25.877 456 256 13 36 478 168 554 9.99e-26 110
MS.gene21954.t1 MTR_2g097467 31.579 209 133 2 56 258 289 493 1.60e-25 110
MS.gene21954.t1 MTR_2g097467 30.201 149 104 0 329 477 505 653 1.17e-17 86.3
MS.gene21954.t1 MTR_5g015950 24.658 438 287 11 56 477 35 445 1.78e-25 108
MS.gene21954.t1 MTR_3g053270 24.658 438 287 11 56 477 35 445 1.78e-25 108
MS.gene21954.t1 MTR_5g058860 25.758 462 249 14 41 477 104 496 2.45e-25 109
MS.gene21954.t1 MTR_2g097473 31.579 209 133 2 56 258 289 493 4.27e-25 109
MS.gene21954.t1 MTR_2g097473 29.530 149 105 0 329 477 505 653 2.42e-16 82.4
MS.gene21954.t1 MTR_4g095500 23.729 472 285 12 38 477 39 467 4.37e-25 108
MS.gene21954.t1 MTR_4g097080 29.091 220 132 4 55 258 132 343 5.44e-25 108
MS.gene21954.t1 MTR_4g097080 30.247 162 106 4 322 478 344 503 2.01e-13 72.8
MS.gene21954.t1 MTR_7g057230 27.586 348 176 10 141 477 374 656 8.50e-24 105
MS.gene21954.t1 MTR_5g015490 29.493 217 145 3 50 258 108 324 1.06e-23 104
MS.gene21954.t1 MTR_5g015490 29.032 155 103 4 329 478 338 490 5.18e-11 65.1
MS.gene21954.t1 MTR_4g076020 29.534 193 120 4 60 249 88 267 2.66e-22 99.4
MS.gene21954.t1 MTR_4g076020 39.456 147 87 2 332 478 285 429 3.77e-21 96.3
MS.gene21954.t1 MTR_2g034250 33.974 156 94 3 328 478 434 585 3.25e-22 100
MS.gene21954.t1 MTR_2g034250 29.032 248 160 6 33 264 186 433 5.60e-20 93.2
MS.gene21954.t1 MTR_1g086970 25.216 464 257 17 36 478 84 478 1.61e-21 97.4
MS.gene21954.t1 MTR_2g099110 26.512 215 148 4 47 252 65 278 1.47e-20 94.4
MS.gene21954.t1 MTR_3g021320 37.500 152 65 3 156 307 1 122 3.40e-20 86.7
MS.gene21954.t1 MTR_6g047750 23.570 437 249 12 57 478 296 662 1.52e-19 92.0
MS.gene21954.t1 MTR_1g096030 27.778 270 157 9 50 288 74 336 3.99e-19 90.1
MS.gene21954.t1 MTR_1g096030 29.143 175 106 4 323 479 286 460 7.54e-19 89.4
MS.gene21954.t1 MTR_4g026485 22.430 428 255 11 57 479 270 625 5.41e-19 90.5
MS.gene21954.t1 MTR_3g072710 28.571 245 135 8 41 255 105 339 5.94e-19 89.7
MS.gene21954.t1 MTR_3g072710 31.707 164 98 5 321 477 343 499 9.79e-14 73.6
MS.gene21954.t1 MTR_8g093070 27.536 207 136 8 53 254 136 333 7.42e-14 73.9
MS.gene21954.t1 MTR_0092s0100 30.303 165 113 1 33 197 349 511 3.51e-13 72.4
MS.gene21954.t1 MTR_0092s0100 30.303 165 113 1 33 197 352 514 3.58e-13 72.4
MS.gene21954.t1 MTR_3g025340 49.180 61 31 0 114 174 9 69 8.64e-13 63.9
MS.gene21954.t1 MTR_8g020840 23.182 440 246 16 50 466 165 535 1.13e-12 70.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21954.t1 AT1G50420 65.914 487 155 7 1 481 1 482 0.0 652
MS.gene21954.t1 AT1G21450 29.302 430 238 6 55 478 224 593 4.69e-54 191
MS.gene21954.t1 AT1G50600 29.476 458 251 12 31 478 131 526 3.15e-53 187
MS.gene21954.t1 AT1G50600 29.476 458 251 12 31 478 206 601 7.63e-53 188
MS.gene21954.t1 AT1G50600 29.476 458 251 12 31 478 202 597 7.78e-53 188
MS.gene21954.t1 AT4G17230 25.951 447 262 7 39 478 141 525 9.27e-46 167
MS.gene21954.t1 AT4G17230 25.951 447 262 7 39 478 141 525 9.27e-46 167
MS.gene21954.t1 AT1G07520 24.099 444 268 6 45 477 143 528 1.30e-43 161
MS.gene21954.t1 AT1G07520 24.099 444 268 6 45 477 143 528 1.30e-43 161
MS.gene21954.t1 AT3G54220 42.995 207 116 1 45 249 282 488 2.77e-43 162
MS.gene21954.t1 AT3G54220 36.301 146 91 2 335 480 509 652 5.07e-23 102
MS.gene21954.t1 AT1G07520 24.099 444 268 6 45 477 307 692 7.13e-43 161
MS.gene21954.t1 AT1G07520 24.099 444 268 6 45 477 308 693 7.26e-43 161
MS.gene21954.t1 AT3G46600 26.097 433 253 6 55 481 3 374 3.87e-41 151
MS.gene21954.t1 AT5G59450 25.688 436 253 9 53 478 224 598 2.50e-40 153
MS.gene21954.t1 AT1G07530 25.408 429 253 7 56 477 396 764 2.51e-40 155
MS.gene21954.t1 AT3G46600 26.097 433 253 6 55 481 179 550 3.52e-40 152
MS.gene21954.t1 AT3G46600 26.097 433 253 6 55 481 211 582 6.15e-40 152
MS.gene21954.t1 AT2G29065 25.513 439 247 10 56 477 252 627 1.17e-38 149
MS.gene21954.t1 AT1G66350 35.616 219 132 4 44 260 143 354 3.68e-36 140
MS.gene21954.t1 AT1G66350 37.179 156 93 2 327 481 358 509 4.71e-31 125
MS.gene21954.t1 AT1G14920 36.161 224 129 4 44 260 160 376 1.34e-34 136
MS.gene21954.t1 AT1G14920 34.868 152 95 2 328 478 381 529 8.71e-27 114
MS.gene21954.t1 AT2G01570 35.683 227 126 5 44 260 212 428 2.36e-34 136
MS.gene21954.t1 AT2G01570 37.500 152 91 3 328 478 433 581 4.77e-27 114
MS.gene21954.t1 AT5G17490 34.404 218 132 4 47 260 151 361 2.72e-34 135
MS.gene21954.t1 AT5G17490 38.562 153 92 2 327 478 365 516 1.84e-27 115
MS.gene21954.t1 AT5G48150 28.975 283 189 4 57 329 123 403 7.99e-34 133
MS.gene21954.t1 AT5G48150 36.709 158 99 1 321 478 334 490 1.01e-26 113
MS.gene21954.t1 AT5G48150 28.975 283 189 4 57 329 123 403 7.99e-34 133
MS.gene21954.t1 AT5G48150 36.709 158 99 1 321 478 334 490 1.01e-26 113
MS.gene21954.t1 AT5G48150 28.975 283 189 4 57 329 123 403 7.99e-34 133
MS.gene21954.t1 AT5G48150 36.709 158 99 1 321 478 334 490 1.01e-26 113
MS.gene21954.t1 AT5G48150 28.975 283 189 4 57 329 123 403 7.99e-34 133
MS.gene21954.t1 AT5G48150 36.709 158 99 1 321 478 334 490 1.01e-26 113
MS.gene21954.t1 AT5G66770 27.169 438 237 13 53 478 217 584 6.69e-33 132
MS.gene21954.t1 AT3G03450 32.287 223 142 4 44 260 171 390 1.61e-32 130
MS.gene21954.t1 AT3G03450 39.355 155 92 2 325 478 392 545 2.55e-31 127
MS.gene21954.t1 AT1G63100 32.110 218 139 3 48 259 244 458 5.92e-32 129
MS.gene21954.t1 AT1G63100 36.196 163 91 3 328 477 466 628 9.38e-25 108
MS.gene21954.t1 AT1G63100 32.110 218 139 3 48 259 269 483 6.37e-32 129
MS.gene21954.t1 AT1G63100 36.196 163 91 3 328 477 491 653 1.24e-24 107
MS.gene21954.t1 AT5G41920 32.338 201 132 3 53 249 40 240 1.40e-31 125
MS.gene21954.t1 AT5G41920 36.774 155 91 3 327 480 254 402 2.08e-20 93.6
MS.gene21954.t1 AT2G37650 24.186 430 256 7 56 478 347 713 6.47e-31 127
MS.gene21954.t1 AT2G37650 24.186 430 256 7 56 478 335 701 8.77e-31 126
MS.gene21954.t1 AT2G04890 33.491 212 136 3 56 267 53 259 1.16e-30 123
MS.gene21954.t1 AT2G04890 40.000 150 89 1 329 478 265 413 9.76e-28 115
MS.gene21954.t1 AT5G52510 25.397 441 233 11 61 478 273 640 3.42e-30 124
MS.gene21954.t1 AT1G55580 36.184 152 96 1 328 478 294 445 3.69e-28 116
MS.gene21954.t1 AT1G55580 24.688 320 196 7 52 329 40 356 6.28e-15 77.4
MS.gene21954.t1 AT3G49950 24.831 443 255 12 52 477 26 407 5.57e-25 107
MS.gene21954.t1 AT2G29060 29.245 212 141 3 56 259 321 531 4.24e-24 106
MS.gene21954.t1 AT2G29060 27.273 154 103 3 329 477 542 691 2.02e-15 79.3
MS.gene21954.t1 AT2G29060 29.245 212 141 3 56 259 284 494 5.07e-24 105
MS.gene21954.t1 AT2G29060 27.273 154 103 3 329 477 505 654 1.68e-15 79.7
MS.gene21954.t1 AT4G37650 26.432 227 135 4 56 258 146 364 1.53e-22 100
MS.gene21954.t1 AT4G37650 26.415 159 107 3 329 478 372 529 6.36e-11 65.1
MS.gene21954.t1 AT4G08250 29.596 223 139 7 47 255 98 316 4.72e-20 93.2
MS.gene21954.t1 AT3G50650 30.876 217 133 4 58 267 177 383 9.09e-20 92.8
MS.gene21954.t1 AT3G50650 28.859 149 99 3 337 478 394 542 1.76e-13 73.2

Find 92 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATAATTCTTGTGGAATTTA+AGG 0.089377 5.1:+2976921 None:intergenic
TATGCTTTCTGAAGAAATTA+TGG 0.230947 5.1:-2976899 MS.gene21954:CDS
TTTGAGTTTCTTGACATAAT+AGG 0.254589 5.1:+2976451 None:intergenic
TGCAGCATTGTTTGATTGTT+TGG 0.259936 5.1:-2976161 MS.gene21954:CDS
CAGCTTCTTCAGAAAGCTTA+TGG 0.264058 5.1:+2976641 None:intergenic
TCATCAGCAGAAAGAGGTTT+TGG 0.271986 5.1:-2976674 MS.gene21954:CDS
AACATACCATAATAGCTTAA+AGG 0.277960 5.1:+2975971 None:intergenic
ACAATGCTGCATAAGAATAT+AGG 0.281076 5.1:+2976173 None:intergenic
TCAAGCTATTGTTGAATCTA+TGG 0.285430 5.1:-2976818 MS.gene21954:CDS
TGCTAAGAACAGGGTTGAAT+TGG 0.312189 5.1:+2976599 None:intergenic
CTCAAATGAGGAGGACCTTC+AGG 0.312197 5.1:+2976709 None:intergenic
ATCTATGGAAGGTGAGAAAA+TGG 0.335503 5.1:-2976803 MS.gene21954:CDS
TCTGCAGTTACATTCCCTTT+TGG 0.339325 5.1:-2976509 MS.gene21954:CDS
AACATCCCTAGAGAGATTAA+AGG 0.354098 5.1:-2976122 MS.gene21954:CDS
AAGCTATTATGGTATGTTGC+AGG 0.357915 5.1:-2975966 MS.gene21954:CDS
AATCACCTAATGATGTGTTT+TGG 0.364016 5.1:+2976395 None:intergenic
TAGTAACTTGCTGGCAAGAT+AGG 0.366004 5.1:-2975880 MS.gene21954:CDS
CAATGCTGCATAAGAATATA+GGG 0.366018 5.1:+2976174 None:intergenic
GACAAACTTCGTGTGAAAAC+CGG 0.371883 5.1:-2976556 MS.gene21954:CDS
AATGTGCCTTTAAGCTATTA+TGG 0.377946 5.1:-2975977 MS.gene21954:CDS
AGACTTGATTCGTGTGGATT+TGG 0.381293 5.1:-2976001 MS.gene21954:CDS
ATGAACCAAAACACATCATT+AGG 0.381360 5.1:-2976400 MS.gene21954:CDS
TCACCTAGCATAGGAGGAAT+AGG 0.416008 5.1:-2977186 MS.gene21954:CDS
TATAGAATGAGAGAAGAAAA+TGG 0.416830 5.1:-2975908 MS.gene21954:CDS
CTGATAGGATACTTAAAACT+TGG 0.427400 5.1:-2976960 MS.gene21954:CDS
AGAAGGTTTCTACAGAGTTA+TGG 0.432910 5.1:-2975941 MS.gene21954:CDS
TCACTGAAGCACTTGCTGAT+AGG 0.433040 5.1:-2976975 MS.gene21954:CDS
ATGGTTGTGTAGTAACTTGC+TGG 0.448692 5.1:-2975889 MS.gene21954:CDS
TCGGTTACAACCATGACTTT+TGG 0.460163 5.1:+2976247 None:intergenic
TCACAACGGTTCAACATTGA+TGG 0.461983 5.1:-2976212 MS.gene21954:CDS
TTGCACAAGTGATTAACAAA+TGG 0.462673 5.1:+2977103 None:intergenic
CAACAACAGCTTGGAGAGCT+AGG 0.464849 5.1:-2975847 MS.gene21954:CDS
TCTCAACCTTTAATCTCTCT+AGG 0.466126 5.1:+2976116 None:intergenic
TGTGCAAACCATGTAGCTTC+TGG 0.466387 5.1:-2977087 MS.gene21954:CDS
ATTCATCATCCAAGGCCAAA+AGG 0.470579 5.1:+2976494 None:intergenic
GATGATGTTGAATCGGGACT+TGG 0.470973 5.1:+2976340 None:intergenic
GTTGTGATTCGAGTCCTGTT+CGG 0.477667 5.1:+2976228 None:intergenic
CAAGACTACCAGAAGCTACA+TGG 0.479369 5.1:+2977079 None:intergenic
GAATCTATGTTACAAGAAGA+AGG 0.480629 5.1:-2977261 MS.gene21954:CDS
AGAATTGAAATCTGAGGAAA+GGG 0.483022 5.1:-2977139 MS.gene21954:CDS
ATATATGCCACCTTCAAGAA+AGG 0.483743 5.1:+2976850 None:intergenic
ATTATGTCAAGAAACTCAAA+TGG 0.483850 5.1:-2976448 MS.gene21954:CDS
CCTCCTCATTTGAGAATCAC+AGG 0.490720 5.1:-2976700 MS.gene21954:CDS
AAGGTTGAGAAGATGCTGTT+TGG 0.498109 5.1:-2976103 MS.gene21954:CDS
AGAAAGCATAATAATTCTTG+TGG 0.500494 5.1:+2976912 None:intergenic
CTCTGCATAGTGTCTCCATC+AGG 0.502156 5.1:+2977012 None:intergenic
AGTCATGGTTGTAACCGAAC+AGG 0.503090 5.1:-2976242 MS.gene21954:CDS
CGACGATGATGATGTTGAAT+CGG 0.503259 5.1:+2976333 None:intergenic
GCTAGGTGAAACTGACATCA+TGG 0.505670 5.1:+2977200 None:intergenic
GAGAATTGAAATCTGAGGAA+AGG 0.506938 5.1:-2977140 MS.gene21954:CDS
TCTCTACTAAGCCATGGATA+AGG 0.507017 5.1:+2977159 None:intergenic
AGGATACTTAAAACTTGGCC+TGG 0.515401 5.1:-2976955 MS.gene21954:CDS
CTACAGAGTTATGGTTGTGA+GGG 0.516522 5.1:-2975932 MS.gene21954:CDS
TCACAGCTTGCAACTCCTGA+TGG 0.516747 5.1:-2977027 MS.gene21954:CDS
TTCATCATCCAAGGCCAAAA+GGG 0.519127 5.1:+2976495 None:intergenic
TTCAATTCTCTACTAAGCCA+TGG 0.520245 5.1:+2977153 None:intergenic
CTCAACCTTTAATCTCTCTA+GGG 0.522051 5.1:+2976117 None:intergenic
TATATGCCACCTTCAAGAAA+GGG 0.525767 5.1:+2976851 None:intergenic
TTGTGAAGGAGCTGAAAGAA+AGG 0.532875 5.1:-2976056 MS.gene21954:CDS
AAAAGAGCAATCCATTGTGC+AGG 0.535750 5.1:+2976751 None:intergenic
AGGGATTCATCAGCAGAAAG+AGG 0.542783 5.1:-2976680 MS.gene21954:CDS
TTCATCTGCTTCAATGAATG+TGG 0.545313 5.1:-2976302 MS.gene21954:CDS
ACTAATTGCAAGCGCCTCGC+CGG 0.552940 5.1:+2976537 None:intergenic
GACGATGATGATGTTGAATC+GGG 0.555408 5.1:+2976334 None:intergenic
ATGGTATGTTGCAGGCAAGA+AGG 0.557823 5.1:-2975958 MS.gene21954:CDS
TTTAAGGCTCTATGAAGTCC+AGG 0.558441 5.1:+2976937 None:intergenic
GATCCTATTCCTCCTATGCT+AGG 0.562234 5.1:+2977183 None:intergenic
TCAGTTTCACCTAGCATAGG+AGG 0.562988 5.1:-2977192 MS.gene21954:CDS
AGAACAGGGTTGAATTGGAA+TGG 0.565131 5.1:+2976604 None:intergenic
TCTACAGAGTTATGGTTGTG+AGG 0.567967 5.1:-2975933 MS.gene21954:CDS
ATTGAAGCAGATGAAGATGC+TGG 0.572949 5.1:+2976310 None:intergenic
GAGTTTGTCACCAAAAGTCA+TGG 0.575658 5.1:-2976257 MS.gene21954:CDS
AAGAACATAATTGCTTGTGA+AGG 0.580873 5.1:-2976070 MS.gene21954:CDS
ATCCCTGTGATTCTCAAATG+AGG 0.584689 5.1:+2976697 None:intergenic
TGAGGATGATTCATCATCCA+AGG 0.589137 5.1:+2976486 None:intergenic
AATCTATGTTACAAGAAGAA+GGG 0.598643 5.1:-2977260 MS.gene21954:CDS
GAATAGGATCACCTTATCCA+TGG 0.599511 5.1:-2977170 MS.gene21954:CDS
GCTATTGTTGAATCTATGGA+AGG 0.607832 5.1:-2976814 MS.gene21954:CDS
TGAGAAGATGCTGTTTGGTG+AGG 0.611876 5.1:-2976098 MS.gene21954:CDS
ATGTCAGTTTCACCTAGCAT+AGG 0.614550 5.1:-2977195 MS.gene21954:CDS
ATCATGGAAAATAACTGTAG+AGG 0.617197 5.1:+2977216 None:intergenic
CTCCTCATTTGAGAATCACA+GGG 0.617281 5.1:-2976699 MS.gene21954:CDS
TTTCTAGCTTGCTAAGAACA+GGG 0.622441 5.1:+2976590 None:intergenic
ACGGTTCAACATTGATGGAG+AGG 0.639461 5.1:-2976207 MS.gene21954:CDS
TAGTAGAGAATTGAAATCTG+AGG 0.644490 5.1:-2977145 MS.gene21954:CDS
TGGATGATGAATCATCCTCA+AGG 0.648643 5.1:-2976483 MS.gene21954:CDS
ATGCTGCTGAACCTGCACAA+TGG 0.650823 5.1:-2976762 MS.gene21954:CDS
GAATTGAAATCTGAGGAAAG+GGG 0.651416 5.1:-2977138 MS.gene21954:CDS
TTTATGAGACTTGATTCGTG+TGG 0.658284 5.1:-2976007 MS.gene21954:CDS
CCTGTGATTCTCAAATGAGG+AGG 0.663636 5.1:+2976700 None:intergenic
ACTTCGTGTGAAAACCGGCG+AGG 0.733150 5.1:-2976551 MS.gene21954:CDS
GAACAGGACTCGAATCACAA+CGG 0.767714 5.1:-2976226 MS.gene21954:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAATTCTTGTGGAATTTA+AGG + chr5.1:2976187-2976206 None:intergenic 20.0%
!! TGATTTACTTGAAAAAGATA+TGG - chr5.1:2976728-2976747 MS.gene21954:CDS 20.0%
! AACATACCATAATAGCTTAA+AGG + chr5.1:2977137-2977156 None:intergenic 25.0%
! AATCTATGTTACAAGAAGAA+GGG - chr5.1:2975845-2975864 MS.gene21954:CDS 25.0%
! ATTATGTCAAGAAACTCAAA+TGG - chr5.1:2976657-2976676 MS.gene21954:CDS 25.0%
! CTTGAAAAAGATATGGTTAA+TGG - chr5.1:2976735-2976754 MS.gene21954:CDS 25.0%
! GACATGAAAAACTTGATAAA+TGG - chr5.1:2977075-2977094 MS.gene21954:CDS 25.0%
! TATAGAATGAGAGAAGAAAA+TGG - chr5.1:2977197-2977216 MS.gene21954:CDS 25.0%
!! AGAAAGCATAATAATTCTTG+TGG + chr5.1:2976196-2976215 None:intergenic 25.0%
!! TATGCTTTCTGAAGAAATTA+TGG - chr5.1:2976206-2976225 MS.gene21954:CDS 25.0%
!! TTTGAGTTTCTTGACATAAT+AGG + chr5.1:2976657-2976676 None:intergenic 25.0%
AAGAACATAATTGCTTGTGA+AGG - chr5.1:2977035-2977054 MS.gene21954:CDS 30.0%
AATGTGCCTTTAAGCTATTA+TGG - chr5.1:2977128-2977147 MS.gene21954:CDS 30.0%
ACAATGCTGCATAAGAATAT+AGG + chr5.1:2976935-2976954 None:intergenic 30.0%
AGAATTGAAATCTGAGGAAA+GGG - chr5.1:2975966-2975985 MS.gene21954:CDS 30.0%
ATCATGGAAAATAACTGTAG+AGG + chr5.1:2975892-2975911 None:intergenic 30.0%
ATGAACCAAAACACATCATT+AGG - chr5.1:2976705-2976724 MS.gene21954:CDS 30.0%
CAATGCTGCATAAGAATATA+GGG + chr5.1:2976934-2976953 None:intergenic 30.0%
CTGATAGGATACTTAAAACT+TGG - chr5.1:2976145-2976164 MS.gene21954:CDS 30.0%
GAATCTATGTTACAAGAAGA+AGG - chr5.1:2975844-2975863 MS.gene21954:CDS 30.0%
TAGTAGAGAATTGAAATCTG+AGG - chr5.1:2975960-2975979 MS.gene21954:CDS 30.0%
TCAAGCTATTGTTGAATCTA+TGG - chr5.1:2976287-2976306 MS.gene21954:CDS 30.0%
TTGCACAAGTGATTAACAAA+TGG + chr5.1:2976005-2976024 None:intergenic 30.0%
! AATCACCTAATGATGTGTTT+TGG + chr5.1:2976713-2976732 None:intergenic 30.0%
! AGAGTTTTCTCAATGCTTTA+TGG - chr5.1:2976826-2976845 MS.gene21954:CDS 30.0%
AACATCCCTAGAGAGATTAA+AGG - chr5.1:2976983-2977002 MS.gene21954:CDS 35.0%
AAGCTATTATGGTATGTTGC+AGG - chr5.1:2977139-2977158 MS.gene21954:CDS 35.0%
AGAAGGTTTCTACAGAGTTA+TGG - chr5.1:2977164-2977183 MS.gene21954:CDS 35.0%
ATATATGCCACCTTCAAGAA+AGG + chr5.1:2976258-2976277 None:intergenic 35.0%
ATCTATGGAAGGTGAGAAAA+TGG - chr5.1:2976302-2976321 MS.gene21954:CDS 35.0%
CTCAACCTTTAATCTCTCTA+GGG + chr5.1:2976991-2977010 None:intergenic 35.0%
GAATTGAAATCTGAGGAAAG+GGG - chr5.1:2975967-2975986 MS.gene21954:CDS 35.0%
GAGAATTGAAATCTGAGGAA+AGG - chr5.1:2975965-2975984 MS.gene21954:CDS 35.0%
GCTATTGTTGAATCTATGGA+AGG - chr5.1:2976291-2976310 MS.gene21954:CDS 35.0%
TATATGCCACCTTCAAGAAA+GGG + chr5.1:2976257-2976276 None:intergenic 35.0%
TCTCAACCTTTAATCTCTCT+AGG + chr5.1:2976992-2977011 None:intergenic 35.0%
TGCAGCATTGTTTGATTGTT+TGG - chr5.1:2976944-2976963 MS.gene21954:CDS 35.0%
TTCAATTCTCTACTAAGCCA+TGG + chr5.1:2975955-2975974 None:intergenic 35.0%
TTCATCTGCTTCAATGAATG+TGG - chr5.1:2976803-2976822 MS.gene21954:CDS 35.0%
! AATAGGAGTTTTTCTCCTTG+AGG + chr5.1:2976640-2976659 None:intergenic 35.0%
! TTTATGAGACTTGATTCGTG+TGG - chr5.1:2977098-2977117 MS.gene21954:CDS 35.0%
!! TTTCTAGCTTGCTAAGAACA+GGG + chr5.1:2976518-2976537 None:intergenic 35.0%
!!! AAAGAGGTTTTGGAGCAAAT+GGG - chr5.1:2976441-2976460 MS.gene21954:CDS 35.0%
!!! CTTTGTTTTCAACAACAGCT+TGG - chr5.1:2977249-2977268 MS.gene21954:CDS 35.0%
!!! TGTGTTTTGGTTCATAAGCA+TGG + chr5.1:2976700-2976719 None:intergenic 35.0%
!!! TTTTCTAGCTTGCTAAGAAC+AGG + chr5.1:2976519-2976538 None:intergenic 35.0%
AAAAGAGCAATCCATTGTGC+AGG + chr5.1:2976357-2976376 None:intergenic 40.0%
AAGGTTGAGAAGATGCTGTT+TGG - chr5.1:2977002-2977021 MS.gene21954:CDS 40.0%
AGAACAGGGTTGAATTGGAA+TGG + chr5.1:2976504-2976523 None:intergenic 40.0%
AGGATACTTAAAACTTGGCC+TGG - chr5.1:2976150-2976169 MS.gene21954:CDS 40.0%
ATCCCTGTGATTCTCAAATG+AGG + chr5.1:2976411-2976430 None:intergenic 40.0%
ATGTCAGTTTCACCTAGCAT+AGG - chr5.1:2975910-2975929 MS.gene21954:CDS 40.0%
ATTCATCATCCAAGGCCAAA+AGG + chr5.1:2976614-2976633 None:intergenic 40.0%
ATTGAAGCAGATGAAGATGC+TGG + chr5.1:2976798-2976817 None:intergenic 40.0%
CAGCTTCTTCAGAAAGCTTA+TGG + chr5.1:2976467-2976486 None:intergenic 40.0%
CTACAGAGTTATGGTTGTGA+GGG - chr5.1:2977173-2977192 MS.gene21954:CDS 40.0%
CTCCTCATTTGAGAATCACA+GGG - chr5.1:2976406-2976425 MS.gene21954:CDS 40.0%
GAATAGGATCACCTTATCCA+TGG - chr5.1:2975935-2975954 MS.gene21954:CDS 40.0%
GACAAACTTCGTGTGAAAAC+CGG - chr5.1:2976549-2976568 MS.gene21954:CDS 40.0%
GAGTTTGTCACCAAAAGTCA+TGG - chr5.1:2976848-2976867 MS.gene21954:CDS 40.0%
TCACAACGGTTCAACATTGA+TGG - chr5.1:2976893-2976912 MS.gene21954:CDS 40.0%
TCTACAGAGTTATGGTTGTG+AGG - chr5.1:2977172-2977191 MS.gene21954:CDS 40.0%
TCTCTACTAAGCCATGGATA+AGG + chr5.1:2975949-2975968 None:intergenic 40.0%
TGAGGATGATTCATCATCCA+AGG + chr5.1:2976622-2976641 None:intergenic 40.0%
TGCTAAGAACAGGGTTGAAT+TGG + chr5.1:2976509-2976528 None:intergenic 40.0%
TGGATGATGAATCATCCTCA+AGG - chr5.1:2976622-2976641 MS.gene21954:CDS 40.0%
TTCATCATCCAAGGCCAAAA+GGG + chr5.1:2976613-2976632 None:intergenic 40.0%
TTGTGAAGGAGCTGAAAGAA+AGG - chr5.1:2977049-2977068 MS.gene21954:CDS 40.0%
! AGACTTGATTCGTGTGGATT+TGG - chr5.1:2977104-2977123 MS.gene21954:CDS 40.0%
! ATGGTTGTGTAGTAACTTGC+TGG - chr5.1:2977216-2977235 MS.gene21954:CDS 40.0%
! CGACGATGATGATGTTGAAT+CGG + chr5.1:2976775-2976794 None:intergenic 40.0%
! GACGATGATGATGTTGAATC+GGG + chr5.1:2976774-2976793 None:intergenic 40.0%
! TCATCAGCAGAAAGAGGTTT+TGG - chr5.1:2976431-2976450 MS.gene21954:CDS 40.0%
! TCGGTTACAACCATGACTTT+TGG + chr5.1:2976861-2976880 None:intergenic 40.0%
! TCTGCAGTTACATTCCCTTT+TGG - chr5.1:2976596-2976615 MS.gene21954:CDS 40.0%
! TGAGCTTTTCCCTTTCTTGA+AGG - chr5.1:2976245-2976264 MS.gene21954:CDS 40.0%
! TTTAAGGCTCTATGAAGTCC+AGG + chr5.1:2976171-2976190 None:intergenic 40.0%
!! TAGTAACTTGCTGGCAAGAT+AGG - chr5.1:2977225-2977244 MS.gene21954:CDS 40.0%
!!! GAAAGAGGTTTTGGAGCAAA+TGG - chr5.1:2976440-2976459 MS.gene21954:CDS 40.0%
ACGGTTCAACATTGATGGAG+AGG - chr5.1:2976898-2976917 MS.gene21954:CDS 45.0%
AGGGATTCATCAGCAGAAAG+AGG - chr5.1:2976425-2976444 MS.gene21954:CDS 45.0%
AGTCATGGTTGTAACCGAAC+AGG - chr5.1:2976863-2976882 MS.gene21954:CDS 45.0%
ATGGTATGTTGCAGGCAAGA+AGG - chr5.1:2977147-2977166 MS.gene21954:CDS 45.0%
CAAGACTACCAGAAGCTACA+TGG + chr5.1:2976029-2976048 None:intergenic 45.0%
CCTCCTCATTTGAGAATCAC+AGG - chr5.1:2976405-2976424 MS.gene21954:CDS 45.0%
CCTGTGATTCTCAAATGAGG+AGG + chr5.1:2976408-2976427 None:intergenic 45.0%
GAACAGGACTCGAATCACAA+CGG - chr5.1:2976879-2976898 MS.gene21954:CDS 45.0%
GATCCTATTCCTCCTATGCT+AGG + chr5.1:2975925-2975944 None:intergenic 45.0%
GATGATGTTGAATCGGGACT+TGG + chr5.1:2976768-2976787 None:intergenic 45.0%
GCTAGGTGAAACTGACATCA+TGG + chr5.1:2975908-2975927 None:intergenic 45.0%
GTTGTGATTCGAGTCCTGTT+CGG + chr5.1:2976880-2976899 None:intergenic 45.0%
TCACCTAGCATAGGAGGAAT+AGG - chr5.1:2975919-2975938 MS.gene21954:CDS 45.0%
TCAGTTTCACCTAGCATAGG+AGG - chr5.1:2975913-2975932 MS.gene21954:CDS 45.0%
TGTGCAAACCATGTAGCTTC+TGG - chr5.1:2976018-2976037 MS.gene21954:CDS 45.0%
! GCTTTTCCCTTTCTTGAAGG+TGG - chr5.1:2976248-2976267 MS.gene21954:CDS 45.0%
! GTTACATTCCCTTTTGGCCT+TGG - chr5.1:2976602-2976621 MS.gene21954:CDS 45.0%
! TCACTGAAGCACTTGCTGAT+AGG - chr5.1:2976130-2976149 MS.gene21954:CDS 45.0%
! TGAGAAGATGCTGTTTGGTG+AGG - chr5.1:2977007-2977026 MS.gene21954:CDS 45.0%
!! GTTTTGAGTGCAAGACCTGA+AGG - chr5.1:2976381-2976400 MS.gene21954:CDS 45.0%
ATGCTGCTGAACCTGCACAA+TGG - chr5.1:2976343-2976362 MS.gene21954:CDS 50.0%
CAACAACAGCTTGGAGAGCT+AGG - chr5.1:2977258-2977277 MS.gene21954:CDS 50.0%
CTCAAATGAGGAGGACCTTC+AGG + chr5.1:2976399-2976418 None:intergenic 50.0%
CTCTGCATAGTGTCTCCATC+AGG + chr5.1:2976096-2976115 None:intergenic 50.0%
TCACAGCTTGCAACTCCTGA+TGG - chr5.1:2976078-2976097 MS.gene21954:CDS 50.0%
ACTAATTGCAAGCGCCTCGC+CGG + chr5.1:2976571-2976590 None:intergenic 55.0%
ACTTCGTGTGAAAACCGGCG+AGG - chr5.1:2976554-2976573 MS.gene21954:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 2975841 2977286 2975841 ID=MS.gene21954
chr5.1 mRNA 2975841 2977286 2975841 ID=MS.gene21954.t1;Parent=MS.gene21954
chr5.1 exon 2975841 2977286 2975841 ID=MS.gene21954.t1.exon1;Parent=MS.gene21954.t1
chr5.1 CDS 2975841 2977286 2975841 ID=cds.MS.gene21954.t1;Parent=MS.gene21954.t1
Gene Sequence

>MS.gene21954

ATGGAATCTATGTTACAAGAAGAAGGGTCTTCATCAGTAACTTGTTCACCTCTACAGTTATTTTCCATGATGTCAGTTTCACCTAGCATAGGAGGAATAGGATCACCTTATCCATGGCTTAGTAGAGAATTGAAATCTGAGGAAAGGGGTTTGTATTTGATCCATTTGTTAATCACTTGTGCAAACCATGTAGCTTCTGGTAGTCTTGAAAATGCAAACACAACACTTGAACAGATTTCACAGCTTGCAACTCCTGATGGAGACACTATGCAGAGAATTGCTGCTTATTTCACTGAAGCACTTGCTGATAGGATACTTAAAACTTGGCCTGGACTTCATAGAGCCTTAAATTCCACAAGAATTATTATGCTTTCTGAAGAAATTATGGTTCAGAAGTTTTTCTTTGAGCTTTTCCCTTTCTTGAAGGTGGCATATATTTTGACAAATCAAGCTATTGTTGAATCTATGGAAGGTGAGAAAATGGTTCATATTATTGATCTTAATGCTGCTGAACCTGCACAATGGATTGCTCTTTTACAAGTTTTGAGTGCAAGACCTGAAGGTCCTCCTCATTTGAGAATCACAGGGATTCATCAGCAGAAAGAGGTTTTGGAGCAAATGGGCCATAAGCTTTCTGAAGAAGCTGAGAAATTAGATATACCATTCCAATTCAACCCTGTTCTTAGCAAGCTAGAAAATCTTGATTTCGACAAACTTCGTGTGAAAACCGGCGAGGCGCTTGCAATTAGTTCTATTCTGCAGTTACATTCCCTTTTGGCCTTGGATGATGAATCATCCTCAAGGAGAAAAACTCCTATTATGTCAAGAAACTCAAATGGACTTCACATGCAAAAAGCCATGCTTATGAACCAAAACACATCATTAGGTGATTTACTTGAAAAAGATATGGTTAATGGCTATAGTCCAAGTCCCGATTCAACATCATCATCGTCGCCAGCATCTTCATCTGCTTCAATGAATGTGGAGAGTTTTCTCAATGCTTTATGGAGTTTGTCACCAAAAGTCATGGTTGTAACCGAACAGGACTCGAATCACAACGGTTCAACATTGATGGAGAGGCTGCTTGAAGCCCTATATTCTTATGCAGCATTGTTTGATTGTTTGGAGTCAACTGTATCAAGAACATCCCTAGAGAGATTAAAGGTTGAGAAGATGCTGTTTGGTGAGGAAATCAAGAACATAATTGCTTGTGAAGGAGCTGAAAGAAAGGAAAGACATGAAAAACTTGATAAATGGTTTATGAGACTTGATTCGTGTGGATTTGGTAATGTGCCTTTAAGCTATTATGGTATGTTGCAGGCAAGAAGGTTTCTACAGAGTTATGGTTGTGAGGGATATAGAATGAGAGAAGAAAATGGTTGTGTAGTAACTTGCTGGCAAGATAGGTCTTTGTTTTCAACAACAGCTTGGAGAGCTAGGAAGTAA
Protein sequence

>MS.gene21954.t1

MESMLQEEGSSSVTCSPLQLFSMMSVSPSIGGIGSPYPWLSRELKSEERGLYLIHLLITCANHVASGSLENANTTLEQISQLATPDGDTMQRIAAYFTEALADRILKTWPGLHRALNSTRIIMLSEEIMVQKFFFELFPFLKVAYILTNQAIVESMEGEKMVHIIDLNAAEPAQWIALLQVLSARPEGPPHLRITGIHQQKEVLEQMGHKLSEEAEKLDIPFQFNPVLSKLENLDFDKLRVKTGEALAISSILQLHSLLALDDESSSRRKTPIMSRNSNGLHMQKAMLMNQNTSLGDLLEKDMVNGYSPSPDSTSSSSPASSSASMNVESFLNALWSLSPKVMVVTEQDSNHNGSTLMERLLEALYSYAALFDCLESTVSRTSLERLKVEKMLFGEEIKNIIACEGAERKERHEKLDKWFMRLDSCGFGNVPLSYYGMLQARRFLQSYGCEGYRMREENGCVVTCWQDRSLFSTTAWRARK