AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene22211


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22211.t1 MTR_3g450500 72.680 194 53 0 5 198 182 375 1.38e-96 285
MS.gene22211.t1 MTR_4g007490 70.466 193 57 0 6 198 181 373 4.86e-95 281
MS.gene22211.t1 MTR_2g019810 40.415 193 111 3 2 193 168 357 6.12e-38 134
MS.gene22211.t1 MTR_2g019840 40.104 192 111 3 5 195 183 371 5.95e-37 132
MS.gene22211.t1 MTR_2g437260 35.385 195 126 0 2 196 173 367 4.51e-33 122
MS.gene22211.t1 MTR_2g436460 34.694 196 125 2 2 196 184 377 6.57e-33 121
MS.gene22211.t1 MTR_4g119550 34.375 192 124 1 6 197 193 382 1.31e-32 120
MS.gene22211.t1 MTR_2g437240 34.848 198 125 2 3 198 208 403 2.76e-32 120
MS.gene22211.t1 MTR_2g436400 37.037 189 117 1 5 193 189 375 1.25e-31 118
MS.gene22211.t1 MTR_2g437030 38.660 194 115 3 5 197 268 458 2.75e-31 118
MS.gene22211.t1 MTR_2g437040 34.197 193 125 1 5 197 182 372 5.07e-31 116
MS.gene22211.t1 MTR_4g119580 34.211 190 123 1 6 195 185 372 7.72e-31 115
MS.gene22211.t1 MTR_2g437080 35.678 199 114 2 5 197 182 372 1.10e-30 115
MS.gene22211.t1 MTR_2g436440 35.417 192 122 1 6 197 189 378 2.04e-30 115
MS.gene22211.t1 MTR_2g437090 35.849 159 102 0 39 197 2 160 2.42e-30 109
MS.gene22211.t1 MTR_2g437100 35.106 188 120 1 6 193 208 393 4.78e-30 114
MS.gene22211.t1 MTR_2g437160 34.031 191 124 1 6 196 208 396 5.42e-30 114
MS.gene22211.t1 MTR_2g437150 34.031 191 124 1 6 196 203 391 3.58e-29 112
MS.gene22211.t1 MTR_2g437160 32.353 204 124 3 7 196 102 305 3.66e-28 107
MS.gene22211.t1 MTR_2g438020 32.984 191 126 1 6 196 250 438 5.77e-28 109
MS.gene22211.t1 MTR_2g437170 32.812 192 127 1 5 196 202 391 8.78e-28 108
MS.gene22211.t1 MTR_2g437200 34.574 188 121 1 6 193 211 396 1.06e-27 107
MS.gene22211.t1 MTR_2g437020 37.736 159 98 1 31 188 206 364 9.20e-27 105
MS.gene22211.t1 MTR_4g120380 31.746 189 127 1 6 194 185 371 2.73e-26 103
MS.gene22211.t1 MTR_2g437180 32.984 191 124 2 6 196 195 381 3.82e-26 103
MS.gene22211.t1 MTR_2g437060 34.896 192 123 2 5 196 197 386 4.98e-26 103
MS.gene22211.t1 MTR_8g081000 28.947 190 135 0 6 195 198 387 1.01e-25 102
MS.gene22211.t1 MTR_8g081000 28.947 190 135 0 6 195 198 387 1.03e-25 102
MS.gene22211.t1 MTR_8g081000 28.947 190 135 0 6 195 198 387 1.23e-25 102
MS.gene22211.t1 MTR_2g437960 31.016 187 127 1 6 192 199 383 3.32e-25 101
MS.gene22211.t1 MTR_2g437120 32.461 191 126 2 6 196 197 384 4.17e-25 101
MS.gene22211.t1 MTR_2g437960 31.016 187 127 1 6 192 199 383 4.48e-25 101
MS.gene22211.t1 MTR_2g437130 31.250 192 130 1 6 197 184 373 3.62e-24 98.2
MS.gene22211.t1 MTR_8g080990 31.088 193 133 0 6 198 185 377 4.17e-24 97.8
MS.gene22211.t1 MTR_2g437990 31.414 191 129 1 6 196 195 383 1.89e-23 96.7
MS.gene22211.t1 MTR_2g437940 30.256 195 134 1 2 196 163 355 1.83e-22 93.6
MS.gene22211.t1 MTR_2g076320 30.890 191 130 1 6 196 196 384 1.10e-21 91.7
MS.gene22211.t1 MTR_4g120380 30.952 168 114 1 6 173 185 350 2.19e-21 90.9
MS.gene22211.t1 MTR_2g438010 29.167 192 134 2 5 196 215 404 3.73e-21 90.1
MS.gene22211.t1 MTR_4g119570 29.474 190 99 2 6 195 77 231 6.33e-20 84.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22211.t1 AT5G07900 39.896 193 115 1 3 194 209 401 6.19e-47 159
MS.gene22211.t1 AT1G21150 35.294 187 120 1 7 192 164 350 3.69e-35 127
MS.gene22211.t1 AT1G21150 35.294 187 120 1 7 192 200 386 8.05e-35 127
MS.gene22211.t1 AT1G21150 35.294 187 120 1 7 192 264 450 2.01e-34 127
MS.gene22211.t1 AT1G62010 27.854 219 121 3 5 187 175 392 3.50e-22 93.2
MS.gene22211.t1 AT1G61980 28.440 218 119 5 5 186 191 407 1.14e-18 83.2
MS.gene22211.t1 AT1G61980 28.440 218 119 5 5 186 191 407 1.14e-18 83.2
MS.gene22211.t1 AT1G61980 28.440 218 119 5 5 186 191 407 1.14e-18 83.2
MS.gene22211.t1 AT1G61970 27.273 220 121 6 5 187 191 408 1.95e-18 82.8
MS.gene22211.t1 AT1G61970 27.273 220 121 6 5 187 191 408 1.95e-18 82.8
MS.gene22211.t1 AT1G61970 27.273 220 121 6 5 187 191 408 1.95e-18 82.8
MS.gene22211.t1 AT1G61970 27.273 220 121 6 5 187 191 408 1.95e-18 82.8
MS.gene22211.t1 AT1G61970 27.273 220 121 6 5 187 191 408 1.95e-18 82.8
MS.gene22211.t1 AT1G61970 27.273 220 121 6 5 187 191 408 1.95e-18 82.8
MS.gene22211.t1 AT1G61970 27.273 220 121 6 5 187 191 408 1.95e-18 82.8
MS.gene22211.t1 AT5G64950 27.273 198 139 2 1 193 179 376 2.24e-18 82.4
MS.gene22211.t1 AT1G61990 26.180 233 132 4 4 197 183 414 1.87e-17 79.7
MS.gene22211.t1 AT1G61990 26.180 233 132 4 4 197 183 414 1.87e-17 79.7
MS.gene22211.t1 AT1G62110 34.752 141 84 4 5 140 193 330 5.33e-17 78.6
MS.gene22211.t1 AT1G62120 26.364 220 123 4 5 187 197 414 1.34e-16 77.4
MS.gene22211.t1 AT3G46950 38.525 122 71 3 5 125 187 305 6.40e-15 72.8
MS.gene22211.t1 AT1G62085 34.043 141 85 4 5 140 196 333 1.02e-14 72.0
MS.gene22211.t1 AT1G62085 34.043 141 85 4 5 140 196 333 1.02e-14 72.0
MS.gene22211.t1 AT1G62150 31.081 148 94 4 5 152 199 338 6.01e-13 67.0
MS.gene22211.t1 AT1G62150 24.352 193 136 3 3 189 265 453 4.25e-11 61.6
MS.gene22211.t1 AT1G61960 25.660 265 121 7 5 195 192 454 7.59e-13 66.6
MS.gene22211.t1 AT5G23930 25.301 249 115 4 5 183 189 436 8.26e-12 63.5

Find 36 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAGACGCACTTCTGTTAT+TGG 0.151097 3.4:-40450483 MS.gene22211:CDS
GTCGAGAGATTTATGTCTTT+TGG 0.222055 3.4:-40450777 MS.gene22211:CDS
TCAAGGTTCCATTTCCCTTT+TGG 0.244439 3.4:-40450981 MS.gene22211:CDS
TATGAAAACAATGAACTTCT+TGG 0.276128 3.4:-40450708 MS.gene22211:CDS
CTGTTATTGGATGCATATAA+AGG 0.351180 3.4:-40450470 MS.gene22211:CDS
GTTAATTCAGTCAAAGAAAT+GGG 0.353526 3.4:-40450905 MS.gene22211:CDS
CAAGCATTCTAGATTTGTTA+AGG 0.370113 3.4:-40450930 MS.gene22211:CDS
ATTGTATACCAAAAGGGAAA+TGG 0.395580 3.4:+40450973 None:intergenic
AGAAGACACTTATCCCTAGA+TGG 0.395958 3.4:-40450619 MS.gene22211:CDS
GTTCAGAATCCCGGGCCATC+TGG 0.397586 3.4:+40450652 None:intergenic
TGTTAATTCAGTCAAAGAAA+TGG 0.398595 3.4:-40450906 MS.gene22211:CDS
ATCTTAACAACTGCCCATCT+AGG 0.399292 3.4:+40450605 None:intergenic
TGGGAAAATTGTATACCAAA+AGG 0.432816 3.4:+40450966 None:intergenic
GAAGACATTGCCAGATGGCC+CGG 0.434278 3.4:-40450662 MS.gene22211:CDS
CAAGTTGCGGTTCAGAATCC+CGG 0.446814 3.4:+40450643 None:intergenic
CGGGATTCTGAACCGCAACT+TGG 0.464726 3.4:-40450642 MS.gene22211:CDS
CTTAACAAATCTAGAATGCT+TGG 0.481915 3.4:+40450931 None:intergenic
GGGAAAATTGTATACCAAAA+GGG 0.484648 3.4:+40450967 None:intergenic
AGGAAGCTTGGTGTGCCTCA+AGG 0.500306 3.4:-40450998 MS.gene22211:CDS
TCTTAACAACTGCCCATCTA+GGG 0.515531 3.4:+40450606 None:intergenic
CTAGCATGAACAAAGAGACT+TGG 0.517246 3.4:-40450835 MS.gene22211:CDS
TGATGCAGTTCAGAATATTG+AGG 0.524450 3.4:-40451026 MS.gene22211:intron
AAGTTGCGGTTCAGAATCCC+GGG 0.557152 3.4:+40450644 None:intergenic
AAGACATTGCCAGATGGCCC+GGG 0.562060 3.4:-40450661 MS.gene22211:CDS
GAAGACACTTATCCCTAGAT+GGG 0.579858 3.4:-40450618 MS.gene22211:CDS
TGCTTGGCGAATGCTACAGT+GGG 0.584692 3.4:+40450947 None:intergenic
AACTTCTTGGTGAATGATGT+GGG 0.587875 3.4:-40450695 MS.gene22211:CDS
TAGCATGAACAAAGAGACTT+GGG 0.602698 3.4:-40450834 MS.gene22211:CDS
CGCAAGAAGACATTGCCAGA+TGG 0.603186 3.4:-40450667 MS.gene22211:CDS
GAACTTCTTGGTGAATGATG+TGG 0.604661 3.4:-40450696 MS.gene22211:CDS
TTAATTCAGTCAAAGAAATG+GGG 0.606609 3.4:-40450904 MS.gene22211:CDS
ATGCTTGGCGAATGCTACAG+TGG 0.637179 3.4:+40450946 None:intergenic
TTTCTCACTAATCGAAATAG+CGG 0.637691 3.4:+40450538 None:intergenic
TCTGAGATCATCACAAACTG+AGG 0.650346 3.4:+40450740 None:intergenic
TAAGTGTCTTCTCCAAGTTG+CGG 0.658300 3.4:+40450630 None:intergenic
AAAAGGGAAATGGAACCTTG+AGG 0.747491 3.4:+40450983 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTTAAGATTTTGAATTCAAA+AGG - chr3.4:40451145-40451164 MS.gene22211:intron 20.0%
!!! TAATTTTTTGAGATTAAGCT+TGG - chr3.4:40450586-40450605 MS.gene22211:CDS 20.0%
! AAAAATTGAGTTTAGGCAAA+AGG + chr3.4:40450511-40450530 None:intergenic 25.0%
! AAAACAAAAAAAGACTTGGA+AGG + chr3.4:40450863-40450882 None:intergenic 25.0%
! AAAATTGAGTTTAGGCAAAA+GGG + chr3.4:40450510-40450529 None:intergenic 25.0%
! GATTGCAAAAAATTGAGTTT+AGG + chr3.4:40450518-40450537 None:intergenic 25.0%
! GTTAATTCAGTCAAAGAAAT+GGG - chr3.4:40450830-40450849 MS.gene22211:CDS 25.0%
! TATGAAAACAATGAACTTCT+TGG - chr3.4:40451027-40451046 MS.gene22211:intron 25.0%
! TGTTAATTCAGTCAAAGAAA+TGG - chr3.4:40450829-40450848 MS.gene22211:CDS 25.0%
! TTAATTCAGTCAAAGAAATG+GGG - chr3.4:40450831-40450850 MS.gene22211:CDS 25.0%
!! ATCAAGATTCAAGATTTTTC+AGG - chr3.4:40450925-40450944 MS.gene22211:CDS 25.0%
!! GATTAGTGAGAAAATGTTTT+TGG - chr3.4:40451207-40451226 MS.gene22211:intron 25.0%
!! TCAAGATTCAAGATTTTTCA+GGG - chr3.4:40450926-40450945 MS.gene22211:CDS 25.0%
!!! AGTGTTACTGATTAGTATTT+TGG + chr3.4:40450567-40450586 None:intergenic 25.0%
!!! GTGTTACTGATTAGTATTTT+GGG + chr3.4:40450566-40450585 None:intergenic 25.0%
!!! GTTTTGATAAAAGCACAAAA+GGG + chr3.4:40450478-40450497 None:intergenic 25.0%
!!! TCAATTTTTTGCAATCCATT+GGG - chr3.4:40450522-40450541 MS.gene22211:CDS 25.0%
!!! TGTTACTGATTAGTATTTTG+GGG + chr3.4:40450565-40450584 None:intergenic 25.0%
!!! TTCCAAGTCTTTTTTTGTTT+TGG - chr3.4:40450862-40450881 MS.gene22211:CDS 25.0%
ATTGTATACCAAAAGGGAAA+TGG + chr3.4:40450765-40450784 None:intergenic 30.0%
CTGTTATTGGATGCATATAA+AGG - chr3.4:40451265-40451284 MS.gene22211:intron 30.0%
GGGAAAATTGTATACCAAAA+GGG + chr3.4:40450771-40450790 None:intergenic 30.0%
TGCCAAAACAAAAAAAGACT+TGG + chr3.4:40450867-40450886 None:intergenic 30.0%
TGGGAAAATTGTATACCAAA+AGG + chr3.4:40450772-40450791 None:intergenic 30.0%
TTTCTCACTAATCGAAATAG+CGG + chr3.4:40451200-40451219 None:intergenic 30.0%
! CTTAACAAATCTAGAATGCT+TGG + chr3.4:40450807-40450826 None:intergenic 30.0%
! TTCAGAATATTGAGGTTTTG+AGG - chr3.4:40450717-40450736 MS.gene22211:CDS 30.0%
!! CAAGATTCAAGATTTTTCAG+GGG - chr3.4:40450927-40450946 MS.gene22211:CDS 30.0%
!! CAAGCATTCTAGATTTGTTA+AGG - chr3.4:40450805-40450824 MS.gene22211:CDS 30.0%
!! GGTTTTGATAAAAGCACAAA+AGG + chr3.4:40450479-40450498 None:intergenic 30.0%
!!! CAATTTTTTGCAATCCATTG+GGG - chr3.4:40450523-40450542 MS.gene22211:CDS 30.0%
!!! CTCAATTTTTTGCAATCCAT+TGG - chr3.4:40450521-40450540 MS.gene22211:CDS 30.0%
AACTTCTTGGTGAATGATGT+GGG - chr3.4:40451040-40451059 MS.gene22211:intron 35.0%
TAGCATGAACAAAGAGACTT+GGG - chr3.4:40450901-40450920 MS.gene22211:CDS 35.0%
TATAGTTCGCAATGACAAAG+AGG - chr3.4:40450646-40450665 MS.gene22211:CDS 35.0%
TGATGCAGTTCAGAATATTG+AGG - chr3.4:40450709-40450728 MS.gene22211:CDS 35.0%
! GTCGAGAGATTTATGTCTTT+TGG - chr3.4:40450958-40450977 MS.gene22211:CDS 35.0%
! TCACAAACTGAGGACATTTT+TGG + chr3.4:40450988-40451007 None:intergenic 35.0%
!!! ATTCAAGATTTTTCAGGGGT+GGG - chr3.4:40450931-40450950 MS.gene22211:CDS 35.0%
AAAAGGGAAATGGAACCTTG+AGG + chr3.4:40450755-40450774 None:intergenic 40.0%
AGAAGACACTTATCCCTAGA+TGG - chr3.4:40451116-40451135 MS.gene22211:intron 40.0%
AGAAGACGCACTTCTGTTAT+TGG - chr3.4:40451252-40451271 MS.gene22211:intron 40.0%
ATCTTAACAACTGCCCATCT+AGG + chr3.4:40451133-40451152 None:intergenic 40.0%
CTAGCATGAACAAAGAGACT+TGG - chr3.4:40450900-40450919 MS.gene22211:CDS 40.0%
CTTCTAATGACATCATAGCG+AGG + chr3.4:40450626-40450645 None:intergenic 40.0%
GAACTTCTTGGTGAATGATG+TGG - chr3.4:40451039-40451058 MS.gene22211:intron 40.0%
GAAGACACTTATCCCTAGAT+GGG - chr3.4:40451117-40451136 MS.gene22211:intron 40.0%
GACATCATAGCGAGGAATAA+TGG + chr3.4:40450618-40450637 None:intergenic 40.0%
TAAGTGTCTTCTCCAAGTTG+CGG + chr3.4:40451108-40451127 None:intergenic 40.0%
TCTTAACAACTGCCCATCTA+GGG + chr3.4:40451132-40451151 None:intergenic 40.0%
! TCAAGGTTCCATTTCCCTTT+TGG - chr3.4:40450754-40450773 MS.gene22211:CDS 40.0%
! TCTGAGATCATCACAAACTG+AGG + chr3.4:40450998-40451017 None:intergenic 40.0%
! TTAGGCAAAAGGGTCTTTTC+AGG + chr3.4:40450500-40450519 None:intergenic 40.0%
!! GTAGTTTCAACTTTGAAGCG+TGG - chr3.4:40450668-40450687 MS.gene22211:CDS 40.0%
!! TAGTATTTTGGGGAGATCAG+TGG + chr3.4:40450555-40450574 None:intergenic 40.0%
!!! ATTGAGGTTTTGAGGAAGCT+TGG - chr3.4:40450725-40450744 MS.gene22211:CDS 40.0%
!!! GATTCAAGATTTTTCAGGGG+TGG - chr3.4:40450930-40450949 MS.gene22211:CDS 40.0%
!!! TTCAAGATTTTTCAGGGGTG+GGG - chr3.4:40450932-40450951 MS.gene22211:CDS 40.0%
ATCAGTGGTTGAAACCCCAA+TGG + chr3.4:40450540-40450559 None:intergenic 45.0%
! TTTGAAGCGTGGAACTAGCT+GGG - chr3.4:40450679-40450698 MS.gene22211:CDS 45.0%
AAGTTGCGGTTCAGAATCCC+GGG + chr3.4:40451094-40451113 None:intergenic 50.0%
ATGCTTGGCGAATGCTACAG+TGG + chr3.4:40450792-40450811 None:intergenic 50.0%
CAAGTTGCGGTTCAGAATCC+CGG + chr3.4:40451095-40451114 None:intergenic 50.0%
CGCAAGAAGACATTGCCAGA+TGG - chr3.4:40451068-40451087 MS.gene22211:intron 50.0%
TGCTTGGCGAATGCTACAGT+GGG + chr3.4:40450791-40450810 None:intergenic 50.0%
!! CTTTGAAGCGTGGAACTAGC+TGG - chr3.4:40450678-40450697 MS.gene22211:CDS 50.0%
!!! AGATTTTTCAGGGGTGGGGA+TGG - chr3.4:40450936-40450955 MS.gene22211:CDS 50.0%
AAGACATTGCCAGATGGCCC+GGG - chr3.4:40451074-40451093 MS.gene22211:intron 55.0%
CGGGATTCTGAACCGCAACT+TGG - chr3.4:40451093-40451112 MS.gene22211:intron 55.0%
GAAGACATTGCCAGATGGCC+CGG - chr3.4:40451073-40451092 MS.gene22211:intron 55.0%
!! AGGAAGCTTGGTGTGCCTCA+AGG - chr3.4:40450737-40450756 MS.gene22211:CDS 55.0%
GTTCAGAATCCCGGGCCATC+TGG + chr3.4:40451086-40451105 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 40450454 40451303 40450454 ID=MS.gene22211
chr3.4 mRNA 40450454 40451303 40450454 ID=MS.gene22211.t1;Parent=MS.gene22211
chr3.4 exon 40451294 40451303 40451294 ID=MS.gene22211.t1.exon1;Parent=MS.gene22211.t1
chr3.4 CDS 40451294 40451303 40451294 ID=cds.MS.gene22211.t1;Parent=MS.gene22211.t1
chr3.4 exon 40450454 40451040 40450454 ID=MS.gene22211.t1.exon2;Parent=MS.gene22211.t1
chr3.4 CDS 40450454 40451040 40450454 ID=cds.MS.gene22211.t1;Parent=MS.gene22211.t1
Gene Sequence

>MS.gene22211

ATGTATCTATGTAAAGAAACACCCTTTTGTGCTTTTATCAAAACCTGAAAAGACCCTTTTGCCTAAACTCAATTTTTTGCAATCCATTGGGGTTTCAACCACTGATCTCCCCAAAATACTAATCAGTAACACTAATTTTTTGAGATTAAGCTTGGAGAAATCCATTATTCCTCGCTATGATGTCATTAGAAGTATAGTTCGCAATGACAAAGAGGTAGTTTCAACTTTGAAGCGTGGAACTAGCTGGGTTGTAAATGATGCAGTTCAGAATATTGAGGTTTTGAGGAAGCTTGGTGTGCCTCAAGGTTCCATTTCCCTTTTGGTATACAATTTTCCCACTGTAGCATTCGCCAAGCATTCTAGATTTGTTAAGGTTGTTAATTCAGTCAAAGAAATGGGGTTTGATCCTTCCAAGTCTTTTTTTGTTTTGGCACTTCGAGTAATTGCTAGCATGAACAAAGAGACTTGGGAATCAAGATTCAAGATTTTTCAGGGGTGGGGATGGTCGAGAGATTTATGTCTTTTGGCTTTCCAAAAATGTCCTCAGTTTGTGATGATCTCAGAGAAAAAGATTATGAAAACAATGAACTTCTTGGTGAATGATGTGGGTCTTACGCAAGAAGACATTGCCAGATGGCCCGGGATTCTGAACCGCAACTTGGAGAAGACACTTATCCCTAGATGGGCAGTTGTTAAGATTTTGAATTCAAAAGGTTTGATAAAGAGTGACTTTCGCTTAACTACCGCTATTTCGATTAGTGAGAAAATGTTTTTGGAGAGATTTGTGACTCGATTTAAAGAAGACGCACTTCTGTTATTGGATGCATATAAAGGTCAGATGTCAGATTGA

Protein sequence

>MS.gene22211.t1

MYLFQNIEVLRKLGVPQGSISLLVYNFPTVAFAKHSRFVKVVNSVKEMGFDPSKSFFVLALRVIASMNKETWESRFKIFQGWGWSRDLCLLAFQKCPQFVMISEKKIMKTMNFLVNDVGLTQEDIARWPGILNRNLEKTLIPRWAVVKILNSKGLIKSDFRLTTAISISEKMFLERFVTRFKEDALLLLDAYKGQMSD