AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene22212

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22212.t1 MTR_3g450500 84.533 375 58 0 1 375 1 375 0.0 635
MS.gene22212.t1 MTR_4g007490 69.129 379 107 4 1 375 1 373 0.0 514
MS.gene22212.t1 MTR_2g019840 38.040 347 207 5 27 372 32 371 4.90e-73 232
MS.gene22212.t1 MTR_2g019810 38.988 336 197 5 36 370 29 357 2.91e-72 229
MS.gene22212.t1 MTR_4g119550 35.821 335 204 4 39 370 52 378 5.23e-67 217
MS.gene22212.t1 MTR_2g437040 37.069 348 210 5 25 370 28 368 5.53e-66 214
MS.gene22212.t1 MTR_2g436460 36.443 343 206 4 33 369 37 373 1.21e-64 210
MS.gene22212.t1 MTR_2g437260 33.134 335 216 2 37 369 35 363 3.89e-61 202
MS.gene22212.t1 MTR_2g437160 34.421 337 211 4 40 372 65 395 1.08e-59 198
MS.gene22212.t1 MTR_2g436400 33.724 341 216 4 33 370 42 375 6.87e-59 196
MS.gene22212.t1 MTR_4g120380 32.635 334 217 3 39 371 45 371 8.21e-59 195
MS.gene22212.t1 MTR_2g437030 35.088 342 214 5 33 372 121 456 1.03e-58 197
MS.gene22212.t1 MTR_2g437080 34.483 348 208 5 33 372 35 370 1.66e-57 192
MS.gene22212.t1 MTR_4g119580 34.524 336 214 2 39 374 45 374 3.35e-57 191
MS.gene22212.t1 MTR_2g437020 35.362 345 206 5 11 352 21 351 3.77e-57 191
MS.gene22212.t1 MTR_2g437120 34.473 351 213 7 27 370 41 381 6.38e-57 191
MS.gene22212.t1 MTR_2g437100 32.941 340 221 4 40 375 62 398 1.18e-56 191
MS.gene22212.t1 MTR_2g437960 32.239 335 216 4 40 369 55 383 5.47e-56 189
MS.gene22212.t1 MTR_2g437180 33.832 334 210 5 40 369 51 377 5.84e-56 188
MS.gene22212.t1 MTR_2g437960 32.239 335 216 4 40 369 55 383 9.49e-56 189
MS.gene22212.t1 MTR_4g120380 32.588 313 203 3 39 350 45 350 1.85e-54 185
MS.gene22212.t1 MTR_2g437060 34.412 340 215 4 33 370 50 383 1.08e-53 182
MS.gene22212.t1 MTR_2g437150 33.134 335 217 4 40 370 57 388 1.31e-53 182
MS.gene22212.t1 MTR_2g437240 30.079 379 221 4 33 373 27 399 1.31e-53 182
MS.gene22212.t1 MTR_2g436440 31.215 362 232 4 11 370 28 374 2.31e-53 181
MS.gene22212.t1 MTR_2g437130 31.165 369 241 3 2 370 14 369 2.03e-52 179
MS.gene22212.t1 MTR_2g076320 31.967 366 240 4 10 369 18 380 2.13e-52 179
MS.gene22212.t1 MTR_2g437200 30.728 371 215 6 37 370 31 396 1.14e-51 177
MS.gene22212.t1 MTR_2g438010 32.229 332 220 4 40 369 72 400 1.33e-51 177
MS.gene22212.t1 MTR_2g437170 31.608 367 238 6 9 370 30 388 1.35e-51 177
MS.gene22212.t1 MTR_2g437990 31.694 366 240 4 10 369 18 379 2.31e-51 177
MS.gene22212.t1 MTR_2g438020 28.804 368 220 5 40 370 73 435 5.59e-45 162
MS.gene22212.t1 MTR_8g081000 29.290 338 220 3 35 372 69 387 7.61e-43 154
MS.gene22212.t1 MTR_8g081000 29.290 338 220 3 35 372 69 387 1.09e-42 153
MS.gene22212.t1 MTR_8g081000 29.290 338 220 3 35 372 69 387 1.21e-42 154
MS.gene22212.t1 MTR_2g437940 29.429 333 202 4 40 370 51 352 6.09e-40 145
MS.gene22212.t1 MTR_8g080990 29.221 308 215 1 68 375 73 377 7.40e-40 145
MS.gene22212.t1 MTR_2g437090 33.553 152 101 0 219 370 5 156 3.77e-26 103
MS.gene22212.t1 MTR_2g437160 30.348 201 126 3 184 370 102 302 9.35e-24 100
MS.gene22212.t1 MTR_2g436370 39.552 134 79 1 33 164 41 174 2.38e-23 97.1
MS.gene22212.t1 MTR_4g119570 27.165 254 146 3 119 372 17 231 1.18e-22 95.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22212.t1 AT5G07900 37.297 370 222 4 11 371 33 401 1.71e-88 273
MS.gene22212.t1 AT1G21150 36.418 335 207 4 38 369 55 386 7.18e-65 211
MS.gene22212.t1 AT1G21150 36.418 335 207 4 38 369 119 450 2.87e-64 212
MS.gene22212.t1 AT1G21150 36.254 331 205 4 42 369 23 350 2.79e-63 206
MS.gene22212.t1 AT1G62120 32.095 377 210 7 21 355 33 405 8.95e-45 160
MS.gene22212.t1 AT1G61970 31.129 363 197 8 36 356 49 400 1.51e-43 156
MS.gene22212.t1 AT1G61970 31.129 363 197 8 36 356 49 400 1.51e-43 156
MS.gene22212.t1 AT1G61970 31.129 363 197 8 36 356 49 400 1.51e-43 156
MS.gene22212.t1 AT1G61970 31.129 363 197 8 36 356 49 400 1.51e-43 156
MS.gene22212.t1 AT1G61970 31.129 363 197 8 36 356 49 400 1.51e-43 156
MS.gene22212.t1 AT1G61970 31.129 363 197 8 36 356 49 400 1.51e-43 156
MS.gene22212.t1 AT1G61970 31.129 363 197 8 36 356 49 400 1.51e-43 156
MS.gene22212.t1 AT1G61980 31.373 357 204 5 35 355 48 399 5.90e-43 154
MS.gene22212.t1 AT1G61980 31.373 357 204 5 35 355 48 399 5.90e-43 154
MS.gene22212.t1 AT1G61980 31.373 357 204 5 35 355 48 399 5.90e-43 154
MS.gene22212.t1 AT1G62010 30.028 363 189 8 36 356 45 384 4.76e-40 147
MS.gene22212.t1 AT5G23930 28.285 449 229 8 7 373 12 449 4.60e-39 145
MS.gene22212.t1 AT1G61990 27.475 404 231 8 12 366 15 405 1.29e-38 143
MS.gene22212.t1 AT1G61990 27.475 404 231 8 12 366 15 405 1.29e-38 143
MS.gene22212.t1 AT1G62150 28.256 407 214 10 33 366 52 453 1.49e-38 144
MS.gene22212.t1 AT3G46950 31.879 298 187 5 25 317 38 324 7.50e-37 139
MS.gene22212.t1 AT1G62085 31.944 288 182 5 25 303 33 315 4.33e-34 131
MS.gene22212.t1 AT1G62085 31.944 288 182 5 25 303 33 315 4.33e-34 131
MS.gene22212.t1 AT1G61960 34.615 260 158 6 36 291 49 300 9.37e-33 127
MS.gene22212.t1 AT1G61960 31.200 125 83 1 253 374 332 456 4.60e-13 70.5
MS.gene22212.t1 AT1G62110 30.189 318 204 6 13 317 18 330 3.82e-31 123
MS.gene22212.t1 AT5G64950 26.776 366 251 6 20 370 13 376 1.71e-30 120
MS.gene22212.t1 AT1G56380 25.275 364 228 10 33 356 10 369 1.62e-23 100
MS.gene22212.t1 AT1G56380 25.275 364 228 10 33 356 21 380 1.87e-23 100
MS.gene22212.t1 AT1G62490 26.354 277 153 6 21 291 74 305 6.06e-17 81.3

Find 68 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAACCTGTTGAAGAGTTTA+AGG 0.145390 3.4:+40443331 None:intergenic
CATAAGTACCTTTATTATTA+TGG 0.157841 3.4:-40442485 MS.gene22212:CDS
GAAAGATTTACCATTGTTAT+TGG 0.167427 3.4:-40442422 MS.gene22212:CDS
TCTTTACATAGATACGTAAT+TGG 0.208101 3.4:+40443241 None:intergenic
AGTGTTCTTAATTAGTATTT+TGG 0.224598 3.4:+40443125 None:intergenic
TCTGAAATCATCATACAATT+TGG 0.226244 3.4:+40442679 None:intergenic
GAAGTTGAATAACAGCATTT+TGG 0.226972 3.4:+40443289 None:intergenic
AACCATTGAATGATAGTTAA+AGG 0.250245 3.4:+40442990 None:intergenic
AAGAGTTTAAGGGCTGTTTC+AGG 0.269433 3.4:+40443342 None:intergenic
ATGCTGTTATTCAACTTCTA+AGG 0.272127 3.4:-40443284 MS.gene22212:CDS
TATGAAAACAATGAACTTCT+TGG 0.276128 3.4:-40442647 MS.gene22212:CDS
ATTCAACTTCTAAGGAACTA+TGG 0.305786 3.4:-40443276 MS.gene22212:CDS
GAACGCTACATTGGGAAAAT+TGG 0.307287 3.4:+40442894 None:intergenic
ATGGTAAATCTTTCTGAAAT+GGG 0.308277 3.4:+40442431 None:intergenic
AATGGTAAATCTTTCTGAAA+TGG 0.311445 3.4:+40442430 None:intergenic
GTCGAGAGATATGTGTCTTT+TGG 0.315308 3.4:-40442716 MS.gene22212:CDS
TGAATCTAGTAATAAGAAAA+TGG 0.325847 3.4:+40443493 None:intergenic
TTTCTCAGCCATAATAATAA+AGG 0.344392 3.4:+40442477 None:intergenic
CAAAGATGGACAAAGAGAAT+TGG 0.346933 3.4:-40442774 MS.gene22212:CDS
TTGTTATTGGATGCATATAA+AGG 0.358352 3.4:-40442409 MS.gene22212:CDS
ATCTTAATAACTGCACATCT+AGG 0.371337 3.4:+40442544 None:intergenic
GTTAATTCAGTCAAGGAAAT+GGG 0.373577 3.4:-40442844 MS.gene22212:CDS
CAACCTGTTGAAGAGTTTAA+GGG 0.399951 3.4:+40443332 None:intergenic
TCAATGTTCCATTTCTCTAC+TGG 0.408911 3.4:-40442920 MS.gene22212:CDS
TGTTAATTCAGTCAAGGAAA+TGG 0.429636 3.4:-40442845 MS.gene22212:CDS
CTTTACATAGATACGTAATT+GGG 0.430873 3.4:+40443242 None:intergenic
GACCTTTAACTATCATTCAA+TGG 0.434376 3.4:-40442992 MS.gene22212:CDS
ATTGGATACCAGTAGAGAAA+TGG 0.437001 3.4:+40442912 None:intergenic
CAGCCCTTAAACTCTTCAAC+AGG 0.443212 3.4:-40443335 MS.gene22212:CDS
TGCTTGGCGAACGCTACATT+GGG 0.443987 3.4:+40442886 None:intergenic
TGCTATTCTGATCCGTAGCT+TGG 0.455085 3.4:-40442581 MS.gene22212:CDS
AAAGATGGACAAAGAGAATT+GGG 0.462885 3.4:-40442773 MS.gene22212:CDS
TCTGTTGGAAACCATCATAA+AGG 0.462943 3.4:-40443411 MS.gene22212:CDS
GAAATTGAACACAAAATTGA+AGG 0.464710 3.4:+40443457 None:intergenic
CTCAACAAATCTAGAATGCT+TGG 0.472071 3.4:+40442870 None:intergenic
ATGCTTGGCGAACGCTACAT+TGG 0.474402 3.4:+40442885 None:intergenic
TAAAGGTGTCACCTTTATGA+TGG 0.475086 3.4:+40443400 None:intergenic
AAAATTGAGTTTAGGCAAAA+GGG 0.475271 3.4:+40443182 None:intergenic
ATCTATTATCCCTCGCTATG+AGG 0.491302 3.4:-40443076 MS.gene22212:CDS
CAACTTTGAAGCGTGGAACA+TGG 0.501029 3.4:-40443014 MS.gene22212:CDS
AAGCTACGGATCAGAATAGC+AGG 0.501991 3.4:+40442583 None:intergenic
CTTAGTTCGCAATGATAAAG+AGG 0.502291 3.4:-40443043 MS.gene22212:CDS
AGAGTTTAAGGGCTGTTTCA+GGG 0.506990 3.4:+40443343 None:intergenic
TCAAACCTTGTTAATTCATG+TGG 0.522908 3.4:-40443372 MS.gene22212:CDS
TCTTAATAACTGCACATCTA+GGG 0.530762 3.4:+40442545 None:intergenic
AACAAGGTTTGACACTGTAA+AGG 0.531106 3.4:+40443383 None:intergenic
CTTACACCACAAGACATTGT+AGG 0.539834 3.4:-40442610 MS.gene22212:CDS
TCATTCTGTTGCAAACATTG+AGG 0.540275 3.4:-40442965 MS.gene22212:CDS
CAAGCATTCTAGATTTGTTG+AGG 0.550123 3.4:-40442869 MS.gene22212:CDS
GAGTTTAAGGGCTGTTTCAG+GGG 0.554750 3.4:+40443344 None:intergenic
TAAGTATCTTCTCCAAGCTA+CGG 0.568984 3.4:+40442569 None:intergenic
ACTTCAAGTAATCGCAAAGA+TGG 0.569887 3.4:-40442788 MS.gene22212:CDS
GTAATAAGAAAATGGTACAT+TGG 0.571657 3.4:+40443501 None:intergenic
TGAGGCTGTTAATTCAGTCA+AGG 0.573328 3.4:-40442851 MS.gene22212:CDS
TGGTAAATCTTTCTGAAATG+GGG 0.580242 3.4:+40442432 None:intergenic
AAATTGAACACAAAATTGAA+GGG 0.586803 3.4:+40443458 None:intergenic
TTCTAATGACCTCATAGCGA+GGG 0.596584 3.4:+40443067 None:intergenic
TTAATTCAGTCAAGGAAATG+GGG 0.604956 3.4:-40442843 MS.gene22212:CDS
AACTTCTTGGTGAACGATGT+GGG 0.606341 3.4:-40442634 MS.gene22212:CDS
AAAACAAAATAAGACATCAA+AGG 0.606684 3.4:+40442814 None:intergenic
ATCAGTGGTTGAAACCCCAA+TGG 0.618102 3.4:+40443152 None:intergenic
GTAGTTTCAACTTTGAAGCG+TGG 0.635683 3.4:-40443021 MS.gene22212:CDS
GAACTTCTTGGTGAACGATG+TGG 0.641423 3.4:-40442635 MS.gene22212:CDS
GGGCATCCTACAATGTCTTG+TGG 0.642181 3.4:+40442604 None:intergenic
TTATATGCATCCAATAACAA+TGG 0.650321 3.4:+40442412 None:intergenic
CTTCTAATGACCTCATAGCG+AGG 0.650510 3.4:+40443066 None:intergenic
AGTAGAGAAATGGAACATTG+AGG 0.673786 3.4:+40442922 None:intergenic
AGCTACGGATCAGAATAGCA+GGG 0.695511 3.4:+40442584 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACAAAATAAGACATCAA+AGG + chr3.4:40443080-40443099 None:intergenic 20.0%
!! AAATTGAACACAAAATTGAA+GGG + chr3.4:40442436-40442455 None:intergenic 20.0%
!! CATAAGTACCTTTATTATTA+TGG - chr3.4:40443406-40443425 MS.gene22212:CDS 20.0%
!! TGAATCTAGTAATAAGAAAA+TGG + chr3.4:40442401-40442420 None:intergenic 20.0%
!!! AGTGTTCTTAATTAGTATTT+TGG + chr3.4:40442769-40442788 None:intergenic 20.0%
!!! GTGTTCTTAATTAGTATTTT+GGG + chr3.4:40442768-40442787 None:intergenic 20.0%
!!! TAATTTTTTGTTATCAAGCT+TGG - chr3.4:40442788-40442807 MS.gene22212:CDS 20.0%
!!! TATTAAGATTTTGAAGTCAA+GGG - chr3.4:40443361-40443380 MS.gene22212:CDS 20.0%
!!! TTATTAAGATTTTGAAGTCA+AGG - chr3.4:40443360-40443379 MS.gene22212:CDS 20.0%
!!! TTTGATGTCTTATTTTGTTT+TGG - chr3.4:40443079-40443098 MS.gene22212:CDS 20.0%
! AAAAACCACATGAATTAACA+AGG + chr3.4:40442527-40442546 None:intergenic 25.0%
! AAAAATTGAGTTTAGGCAAA+AGG + chr3.4:40442713-40442732 None:intergenic 25.0%
! AAAATTGAGTTTAGGCAAAA+GGG + chr3.4:40442712-40442731 None:intergenic 25.0%
! AACCATTGAATGATAGTTAA+AGG + chr3.4:40442904-40442923 None:intergenic 25.0%
! CTTTACATAGATACGTAATT+GGG + chr3.4:40442652-40442671 None:intergenic 25.0%
! GAAAGATTTACCATTGTTAT+TGG - chr3.4:40443469-40443488 MS.gene22212:CDS 25.0%
! GAAATTGAACACAAAATTGA+AGG + chr3.4:40442437-40442456 None:intergenic 25.0%
! GATTGCAAAAAATTGAGTTT+AGG + chr3.4:40442720-40442739 None:intergenic 25.0%
! TATGAAAACAATGAACTTCT+TGG - chr3.4:40443244-40443263 MS.gene22212:CDS 25.0%
! TCTTTACATAGATACGTAAT+TGG + chr3.4:40442653-40442672 None:intergenic 25.0%
! TTATATGCATCCAATAACAA+TGG + chr3.4:40443482-40443501 None:intergenic 25.0%
! TTTCTCAGCCATAATAATAA+AGG + chr3.4:40443417-40443436 None:intergenic 25.0%
!! AAGTTGAATAACAGCATTTT+GGG + chr3.4:40442604-40442623 None:intergenic 25.0%
!! AATGGTAAATCTTTCTGAAA+TGG + chr3.4:40443464-40443483 None:intergenic 25.0%
!! TCTGAAATCATCATACAATT+TGG + chr3.4:40443215-40443234 None:intergenic 25.0%
!! TTGTTATTGGATGCATATAA+AGG - chr3.4:40443482-40443501 MS.gene22212:CDS 25.0%
!!! ATGGTAAATCTTTCTGAAAT+GGG + chr3.4:40443463-40443482 None:intergenic 25.0%
!!! ATTAAGATTTTGAAGTCAAG+GGG - chr3.4:40443362-40443381 MS.gene22212:CDS 25.0%
!!! CAAAAATCAAGATTTTTGAG+AGG - chr3.4:40443144-40443163 MS.gene22212:CDS 25.0%
!!! GTTTTGAAAAAAGTACAGAA+GGG + chr3.4:40442680-40442699 None:intergenic 25.0%
!!! TCAATTTTTTGCAATCCATT+GGG - chr3.4:40442724-40442743 MS.gene22212:CDS 25.0%
AAAGATGGACAAAGAGAATT+GGG - chr3.4:40443118-40443137 MS.gene22212:CDS 30.0%
ATCTTAATAACTGCACATCT+AGG + chr3.4:40443350-40443369 None:intergenic 30.0%
ATGCTGTTATTCAACTTCTA+AGG - chr3.4:40442607-40442626 MS.gene22212:CDS 30.0%
GACCTTTAACTATCATTCAA+TGG - chr3.4:40442899-40442918 MS.gene22212:CDS 30.0%
GTTAATTCAGTCAAGGAAAT+GGG - chr3.4:40443047-40443066 MS.gene22212:CDS 30.0%
TCAAACCTTGTTAATTCATG+TGG - chr3.4:40442519-40442538 MS.gene22212:CDS 30.0%
TCTTAATAACTGCACATCTA+GGG + chr3.4:40443349-40443368 None:intergenic 30.0%
TGTTAATTCAGTCAAGGAAA+TGG - chr3.4:40443046-40443065 MS.gene22212:CDS 30.0%
TTAATTCAGTCAAGGAAATG+GGG - chr3.4:40443048-40443067 MS.gene22212:CDS 30.0%
! ATTCAACTTCTAAGGAACTA+TGG - chr3.4:40442615-40442634 MS.gene22212:CDS 30.0%
! GAAGTTGAATAACAGCATTT+TGG + chr3.4:40442605-40442624 None:intergenic 30.0%
! TATGGCTGAGAAAACATTTT+TGG - chr3.4:40443424-40443443 MS.gene22212:CDS 30.0%
!! AAATCAAGATTTTTGAGAGG+TGG - chr3.4:40443147-40443166 MS.gene22212:CDS 30.0%
!! AATCAAGATTTTTGAGAGGT+GGG - chr3.4:40443148-40443167 MS.gene22212:CDS 30.0%
!! TCATACAATTTGGCTGTTTT+TGG + chr3.4:40443205-40443224 None:intergenic 30.0%
!! TGGTAAATCTTTCTGAAATG+GGG + chr3.4:40443462-40443481 None:intergenic 30.0%
!!! ATTGAGGTTTTGAGAAAACT+TGG - chr3.4:40442942-40442961 MS.gene22212:CDS 30.0%
!!! CAATTTTTTGCAATCCATTG+GGG - chr3.4:40442725-40442744 MS.gene22212:CDS 30.0%
!!! CTCAATTTTTTGCAATCCAT+TGG - chr3.4:40442723-40442742 MS.gene22212:CDS 30.0%
!!! GGTTTTGAAAAAAGTACAGA+AGG + chr3.4:40442681-40442700 None:intergenic 30.0%
AACAAGGTTTGACACTGTAA+AGG + chr3.4:40442511-40442530 None:intergenic 35.0%
ACTTCAAGTAATCGCAAAGA+TGG - chr3.4:40443103-40443122 MS.gene22212:CDS 35.0%
AGTAGAGAAATGGAACATTG+AGG + chr3.4:40442972-40442991 None:intergenic 35.0%
ATTGGATACCAGTAGAGAAA+TGG + chr3.4:40442982-40443001 None:intergenic 35.0%
CAAAGATGGACAAAGAGAAT+TGG - chr3.4:40443117-40443136 MS.gene22212:CDS 35.0%
CAACCTGTTGAAGAGTTTAA+GGG + chr3.4:40442562-40442581 None:intergenic 35.0%
CTTAGTTCGCAATGATAAAG+AGG - chr3.4:40442848-40442867 MS.gene22212:CDS 35.0%
TAAGTATCTTCTCCAAGCTA+CGG + chr3.4:40443325-40443344 None:intergenic 35.0%
TCAACCTGTTGAAGAGTTTA+AGG + chr3.4:40442563-40442582 None:intergenic 35.0%
TCAATGTTCCATTTCTCTAC+TGG - chr3.4:40442971-40442990 MS.gene22212:CDS 35.0%
TCATTCTGTTGCAAACATTG+AGG - chr3.4:40442926-40442945 MS.gene22212:CDS 35.0%
TCTGTTGGAAACCATCATAA+AGG - chr3.4:40442480-40442499 MS.gene22212:CDS 35.0%
! ATAACAGCATTTTGGGCATT+AGG + chr3.4:40442597-40442616 None:intergenic 35.0%
! CTCAACAAATCTAGAATGCT+TGG + chr3.4:40443024-40443043 None:intergenic 35.0%
! TAACAGCATTTTGGGCATTA+GGG + chr3.4:40442596-40442615 None:intergenic 35.0%
! TTTTCTCGTTCTTTCTCTGT+TGG - chr3.4:40442465-40442484 MS.gene22212:CDS 35.0%
!! ATCAAGATTTTTGAGAGGTG+GGG - chr3.4:40443149-40443168 MS.gene22212:CDS 35.0%
!! CAAGCATTCTAGATTTGTTG+AGG - chr3.4:40443022-40443041 MS.gene22212:CDS 35.0%
!! TAAAGGTGTCACCTTTATGA+TGG + chr3.4:40442494-40442513 None:intergenic 35.0%
!! TAGTATTTTGGGAAGATCAG+TGG + chr3.4:40442757-40442776 None:intergenic 35.0%
AACTTCTTGGTGAACGATGT+GGG - chr3.4:40443257-40443276 MS.gene22212:CDS 40.0%
ATCTATTATCCCTCGCTATG+AGG - chr3.4:40442815-40442834 MS.gene22212:CDS 40.0%
CTTACACCACAAGACATTGT+AGG - chr3.4:40443281-40443300 MS.gene22212:CDS 40.0%
GAACGCTACATTGGGAAAAT+TGG + chr3.4:40443000-40443019 None:intergenic 40.0%
TGAGGCTGTTAATTCAGTCA+AGG - chr3.4:40443040-40443059 MS.gene22212:CDS 40.0%
TTCTAATGACCTCATAGCGA+GGG + chr3.4:40442827-40442846 None:intergenic 40.0%
! AAGAGTTTAAGGGCTGTTTC+AGG + chr3.4:40442552-40442571 None:intergenic 40.0%
! AGAGTTTAAGGGCTGTTTCA+GGG + chr3.4:40442551-40442570 None:intergenic 40.0%
! GTCGAGAGATATGTGTCTTT+TGG - chr3.4:40443175-40443194 MS.gene22212:CDS 40.0%
! TTAGGCAAAAGGGTCTTTTC+AGG + chr3.4:40442702-40442721 None:intergenic 40.0%
!! GTAGTTTCAACTTTGAAGCG+TGG - chr3.4:40442870-40442889 MS.gene22212:CDS 40.0%
AAGCTACGGATCAGAATAGC+AGG + chr3.4:40443311-40443330 None:intergenic 45.0%
AGCTACGGATCAGAATAGCA+GGG + chr3.4:40443310-40443329 None:intergenic 45.0%
ATCAGTGGTTGAAACCCCAA+TGG + chr3.4:40442742-40442761 None:intergenic 45.0%
CAGCCCTTAAACTCTTCAAC+AGG - chr3.4:40442556-40442575 MS.gene22212:CDS 45.0%
CTTCTAATGACCTCATAGCG+AGG + chr3.4:40442828-40442847 None:intergenic 45.0%
GAACTTCTTGGTGAACGATG+TGG - chr3.4:40443256-40443275 MS.gene22212:CDS 45.0%
TGCTATTCTGATCCGTAGCT+TGG - chr3.4:40443310-40443329 MS.gene22212:CDS 45.0%
! GAGTTTAAGGGCTGTTTCAG+GGG + chr3.4:40442550-40442569 None:intergenic 45.0%
!! AGATTTTTGAGAGGTGGGGT+TGG - chr3.4:40443153-40443172 MS.gene22212:CDS 45.0%
!! CAACTTTGAAGCGTGGAACA+TGG - chr3.4:40442877-40442896 MS.gene22212:CDS 45.0%
ATGCTTGGCGAACGCTACAT+TGG + chr3.4:40443009-40443028 None:intergenic 50.0%
GGGCATCCTACAATGTCTTG+TGG + chr3.4:40443290-40443309 None:intergenic 50.0%
TGCTTGGCGAACGCTACATT+GGG + chr3.4:40443008-40443027 None:intergenic 50.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 40442393 40443520 40442393 ID=MS.gene22212
chr3.4 mRNA 40442393 40443520 40442393 ID=MS.gene22212.t1;Parent=MS.gene22212
chr3.4 exon 40442393 40443520 40442393 ID=MS.gene22212.t1.exon1;Parent=MS.gene22212.t1
chr3.4 CDS 40442393 40443520 40442393 ID=cds.MS.gene22212.t1;Parent=MS.gene22212.t1
Gene Sequence

>MS.gene22212

ATGTACCATTTTCTTATTACTAGATTCACTCATCATAGAACCCTTCAATTTTGTGTTCAATTTCGCAATGATTTTTCTCGTTCTTTCTCTGTTGGAAACCATCATAAAGGTGACACCTTTACAGTGTCAAACCTTGTTAATTCATGTGGTTTTTCCCCTGAAACAGCCCTTAAACTCTTCAACAGGTTGAAACGCAAAAACCCTAATGCCCAAAATGCTGTTATTCAACTTCTAAGGAACTATGGTTTCTCTGATTCCCAATTACGTATCTATGTAAAGAAACACCCTTCTGTACTTTTTTCAAAACCTGAAAAGACCCTTTTGCCTAAACTCAATTTTTTGCAATCCATTGGGGTTTCAACCACTGATCTTCCCAAAATACTAATTAAGAACACTAATTTTTTGTTATCAAGCTTGGAGAAATCTATTATCCCTCGCTATGAGGTCATTAGAAGCTTAGTTCGCAATGATAAAGAGGTAGTTTCAACTTTGAAGCGTGGAACATGGACCTTTAACTATCATTCAATGGTTAATCATTCTGTTGCAAACATTGAGGTTTTGAGAAAACTTGGCGTGCCTCAATGTTCCATTTCTCTACTGGTATCCAATTTTCCCAATGTAGCGTTCGCCAAGCATTCTAGATTTGTTGAGGCTGTTAATTCAGTCAAGGAAATGGGGTTTGATCCTTTGATGTCTTATTTTGTTTTGGCACTTCAAGTAATCGCAAAGATGGACAAAGAGAATTGGGAATCAAAAATCAAGATTTTTGAGAGGTGGGGTTGGTCGAGAGATATGTGTCTTTTGGCTTTCCAAAAACAGCCAAATTGTATGATGATTTCAGAAAAAAAGATTATGAAAACAATGAACTTCTTGGTGAACGATGTGGGTCTTACACCACAAGACATTGTAGGATGCCCTGCTATTCTGATCCGTAGCTTGGAGAAGATACTTATCCCTAGATGTGCAGTTATTAAGATTTTGAAGTCAAGGGGTTTGATAAAGAGTAGTTTGCGCATAAGTACCTTTATTATTATGGCTGAGAAAACATTTTTGGAGCGATATGTAACCCCATTTCAGAAAGATTTACCATTGTTATTGGATGCATATAAAGGTCAGAATTCAGATTGA
Protein sequence

>MS.gene22212.t1

MYHFLITRFTHHRTLQFCVQFRNDFSRSFSVGNHHKGDTFTVSNLVNSCGFSPETALKLFNRLKRKNPNAQNAVIQLLRNYGFSDSQLRIYVKKHPSVLFSKPEKTLLPKLNFLQSIGVSTTDLPKILIKNTNFLLSSLEKSIIPRYEVIRSLVRNDKEVVSTLKRGTWTFNYHSMVNHSVANIEVLRKLGVPQCSISLLVSNFPNVAFAKHSRFVEAVNSVKEMGFDPLMSYFVLALQVIAKMDKENWESKIKIFERWGWSRDMCLLAFQKQPNCMMISEKKIMKTMNFLVNDVGLTPQDIVGCPAILIRSLEKILIPRCAVIKILKSRGLIKSSLRISTFIIMAEKTFLERYVTPFQKDLPLLLDAYKGQNSD