AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene22444

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22444.t1 MTR_2g099990 63.251 283 84 4 33 302 45 320 4.08e-119 354
MS.gene22444.t1 MTR_4g059870 73.756 221 45 6 7 218 6 222 2.53e-115 345
MS.gene22444.t1 MTR_8g017210 56.798 331 104 8 27 329 55 374 1.47e-112 339
MS.gene22444.t1 MTR_1g094115 87.500 168 20 1 34 200 47 214 2.38e-112 339
MS.gene22444.t1 MTR_1g112270 86.875 160 21 0 37 196 47 206 2.59e-108 330
MS.gene22444.t1 MTR_1g016010 61.484 283 86 10 30 308 38 301 2.47e-107 324
MS.gene22444.t1 MTR_2g093960 65.823 237 61 7 34 265 32 253 5.70e-105 317
MS.gene22444.t1 MTR_1g112270 81.287 171 21 1 37 196 47 217 1.16e-104 321
MS.gene22444.t1 MTR_2g090745 45.854 410 147 10 42 380 15 420 7.10e-104 314
MS.gene22444.t1 MTR_2g099990 61.240 258 80 4 58 302 1 251 5.47e-102 308
MS.gene22444.t1 MTR_1g094115 76.786 168 21 2 34 200 47 197 3.35e-94 292
MS.gene22444.t1 MTR_2g017720 77.391 115 21 1 44 158 323 432 2.78e-56 191
MS.gene22444.t1 MTR_3g102000 66.019 103 28 2 61 159 36 135 9.47e-39 139
MS.gene22444.t1 MTR_5g030560 86.667 45 6 0 44 88 16 60 2.52e-22 90.5
MS.gene22444.t1 MTR_1g093095 34.503 171 95 5 28 186 84 249 3.89e-21 93.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22444.t1 AT5G03150 60.841 309 73 9 3 283 4 292 6.81e-116 347
MS.gene22444.t1 AT3G50700 85.549 173 25 0 24 196 24 196 1.03e-113 340
MS.gene22444.t1 AT5G66730 75.728 206 43 2 11 209 8 213 8.41e-112 337
MS.gene22444.t1 AT5G44160 53.239 355 107 9 28 329 32 380 1.37e-111 335
MS.gene22444.t1 AT1G55110 62.759 290 89 7 30 306 58 341 2.79e-111 334
MS.gene22444.t1 AT1G55110 62.759 290 89 7 30 306 58 341 2.79e-111 334
MS.gene22444.t1 AT1G55110 62.759 290 89 7 30 306 58 341 2.79e-111 334
MS.gene22444.t1 AT3G45260 84.181 177 23 2 33 204 38 214 8.43e-109 327
MS.gene22444.t1 AT3G45260 84.181 177 23 2 33 204 38 214 8.43e-109 327
MS.gene22444.t1 AT1G03840 78.610 187 38 2 28 213 36 221 9.32e-109 329
MS.gene22444.t1 AT3G13810 86.826 167 21 1 31 196 48 214 1.90e-106 323
MS.gene22444.t1 AT1G14580 82.738 168 26 1 29 196 51 215 2.18e-106 322
MS.gene22444.t1 AT1G14580 82.738 168 26 1 29 196 51 215 2.18e-106 322
MS.gene22444.t1 AT3G13810 86.826 167 21 1 31 196 67 233 2.70e-106 323
MS.gene22444.t1 AT3G13810 86.826 167 21 1 31 196 67 233 2.70e-106 323
MS.gene22444.t1 AT3G13810 86.826 167 21 1 31 196 62 228 2.78e-106 323
MS.gene22444.t1 AT2G02070 83.529 170 26 1 34 203 52 219 3.79e-106 325
MS.gene22444.t1 AT2G02070 83.529 170 26 1 34 203 52 219 3.79e-106 325
MS.gene22444.t1 AT1G14580 82.738 168 26 1 29 196 65 229 3.98e-106 322
MS.gene22444.t1 AT1G03840 77.540 187 38 3 28 213 36 219 4.66e-105 320
MS.gene22444.t1 AT4G02670 81.714 175 31 1 34 207 53 227 5.38e-105 316
MS.gene22444.t1 AT2G02080 70.647 201 46 3 7 197 20 217 5.92e-105 320
MS.gene22444.t1 AT2G02080 70.647 201 46 3 7 197 20 217 5.92e-105 320
MS.gene22444.t1 AT2G02080 70.647 201 46 3 7 197 20 217 5.92e-105 320
MS.gene22444.t1 AT2G02080 70.647 201 46 3 7 197 20 217 5.92e-105 320
MS.gene22444.t1 AT4G02670 81.714 175 31 1 34 207 55 229 6.39e-105 316
MS.gene22444.t1 AT5G60470 75.521 192 38 3 21 203 24 215 2.45e-104 316
MS.gene22444.t1 AT5G60470 75.521 192 38 3 21 203 20 211 3.21e-104 316
MS.gene22444.t1 AT5G44160 52.212 339 104 8 44 329 8 341 1.83e-103 313
MS.gene22444.t1 AT3G13810 88.235 153 17 1 45 196 68 220 3.91e-98 302
MS.gene22444.t1 AT3G13810 88.235 153 17 1 45 196 82 234 6.46e-98 301
MS.gene22444.t1 AT5G60470 80.745 161 29 2 45 203 46 206 6.94e-95 292
MS.gene22444.t1 AT2G02080 81.295 139 26 0 58 196 1 139 1.69e-88 275
MS.gene22444.t1 AT2G02080 81.295 139 26 0 58 196 1 139 1.69e-88 275
MS.gene22444.t1 AT1G68130 56.842 190 74 3 32 219 39 222 7.23e-75 239
MS.gene22444.t1 AT1G25250 64.780 159 53 2 32 187 31 189 3.41e-74 236
MS.gene22444.t1 AT2G01940 63.190 163 58 2 32 192 42 204 9.56e-74 237
MS.gene22444.t1 AT2G01940 62.195 164 59 3 32 192 42 205 3.63e-70 228
MS.gene22444.t1 AT1G25250 65.306 147 48 2 44 187 20 166 3.34e-69 223
MS.gene22444.t1 AT1G68130 52.817 142 59 3 80 219 1 136 9.74e-46 160
MS.gene22444.t1 AT1G25250 62.162 111 39 2 80 187 1 111 2.46e-44 156
MS.gene22444.t1 AT2G01940 60.870 115 43 2 80 192 1 115 2.69e-44 157
MS.gene22444.t1 AT1G34370 29.918 244 124 8 43 244 14 252 9.17e-25 103
MS.gene22444.t1 AT1G34370 29.918 244 124 8 43 244 224 462 3.08e-24 104
MS.gene22444.t1 AT1G34370 29.918 244 124 8 43 244 224 462 3.08e-24 104
MS.gene22444.t1 AT5G22890 32.530 166 96 4 35 186 191 354 1.31e-22 99.0
MS.gene22444.t1 AT1G13290 34.211 152 88 5 55 195 93 243 1.79e-19 88.6
MS.gene22444.t1 AT1G08290 35.849 159 85 7 44 186 155 312 1.19e-18 86.7
MS.gene22444.t1 AT1G34790 33.333 180 104 7 47 218 129 300 1.24e-18 86.3
MS.gene22444.t1 AT3G20880 34.247 146 86 5 50 186 243 387 2.76e-18 86.7
MS.gene22444.t1 AT1G51220 33.562 146 87 5 50 186 165 309 4.04e-18 85.1
MS.gene22444.t1 AT3G57670 34.247 146 86 5 50 186 204 348 6.10e-18 85.1

Find 76 sgRNAs with CRISPR-Local

Find 114 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCTGGTTTGTGGTGGATT+AGG 0.210609 7.1:-8465268 None:intergenic
CCATGATGCGGCCAGGGCCT+TGG 0.232187 7.1:+8465632 MS.gene22444:CDS
AGATTGAACTGCATATTTCT+TGG 0.249648 7.1:-8465727 None:intergenic
TGCTCACAAATGAATATTCT+TGG 0.256662 7.1:+8465181 None:intergenic
GCTAAAATGTTTCTTTATTC+CGG 0.260327 7.1:-8465664 None:intergenic
TAGATTTCTCTTCTTCTTGC+TGG 0.261742 7.1:-8465284 None:intergenic
CTTCAGAAAGCAGCTCAAAT+TGG 0.266190 7.1:+8466891 MS.gene22444:CDS
ACTCATCATCGATGAACTTA+TGG 0.281450 7.1:-8467174 None:intergenic
TGCTGGTTTGTGGTGGATTA+GGG 0.287287 7.1:-8465267 None:intergenic
TATTAACCTCATCATGGTTT+TGG 0.287752 7.1:-8467001 None:intergenic
TTCGTCGCCATTAGAGTCTT+TGG 0.293737 7.1:-8465453 None:intergenic
GCTGAAGATTCTGATCTCTT+TGG 0.295858 7.1:-8465509 None:intergenic
GTTTGTGGTGGATTAGGGTT+AGG 0.305769 7.1:-8465262 None:intergenic
CAACAAAGAACCAAGGAAAA+AGG 0.327722 7.1:+8465578 MS.gene22444:CDS
CTCTTCTTCTTGCTGGTTTG+TGG 0.328936 7.1:-8465277 None:intergenic
TATTTGACGACTCTTCATTC+TGG 0.329154 7.1:-8466629 None:intergenic
TTAATATTACCTGGTGTTCC+TGG 0.358554 7.1:-8465307 None:intergenic
GAAAACATGGTGAGAAGAAA+TGG 0.363622 7.1:+8465688 MS.gene22444:CDS
AAACCATGATGAGGTTAATA+TGG 0.368833 7.1:+8467004 MS.gene22444:CDS
CATGATGCGGCCAGGGCCTT+GGG 0.370145 7.1:+8465633 MS.gene22444:CDS
TTCTGGAAATTTAGAGTTGT+AGG 0.385136 7.1:-8466612 None:intergenic
TTCAACCACAATGACAAGTT+TGG 0.398343 7.1:-8466753 None:intergenic
GCTCTTGAGGTAGAAATTGA+TGG 0.404206 7.1:-8467136 None:intergenic
GTGTCCACCATGATGCGGCC+AGG 0.413040 7.1:+8465625 MS.gene22444:CDS
TCTTTCTCCAAAGACTCTAA+TGG 0.414643 7.1:+8465446 MS.gene22444:CDS
TGCATATTTCGCAGACGAAT+CGG 0.417602 7.1:-8465476 None:intergenic
TTTGTGGTGGATTAGGGTTA+GGG 0.429876 7.1:-8465261 None:intergenic
AATATGCAGTTCAATCTGAT+TGG 0.433651 7.1:+8465733 MS.gene22444:CDS
TGGTGGACACAGGTCTTCTC+CGG 0.437364 7.1:-8465612 None:intergenic
GACCTTTGCTTTAGCTTCCA+TGG 0.442243 7.1:-8465555 None:intergenic
AGTTTAAATCAATTCAATGC+TGG 0.449709 7.1:+8467194 MS.gene22444:CDS
TAGTAGTAGTAGTGCAGCTA+TGG 0.457270 7.1:+8467070 MS.gene22444:CDS
TTGAAGTTTGTCAAAACCTC+CGG 0.458884 7.1:-8465230 None:intergenic
GGTGAGGTCACCCAAGGCCC+TGG 0.470064 7.1:-8465643 None:intergenic
GTGGAAGAGGATAGTGAAAA+GGG 0.477404 7.1:-8465208 None:intergenic
TTCTTCTTGCTGGTTTGTGG+TGG 0.478291 7.1:-8465274 None:intergenic
CTATGGTTGGTAATTCTAGT+GGG 0.487415 7.1:+8467087 MS.gene22444:CDS
GTTTAAATCAATTCAATGCT+GGG 0.488084 7.1:+8467195 MS.gene22444:CDS
TTTGCTTTAGCTTCCATGGT+AGG 0.488944 7.1:-8465551 None:intergenic
GTCAAAACCTCCGGTGGAAG+AGG 0.490780 7.1:-8465221 None:intergenic
AGGGCCTTGGGTGACCTCAC+CGG 0.495108 7.1:+8465645 MS.gene22444:CDS
CCAAGGCCCTGGCCGCATCA+TGG 0.496782 7.1:-8465632 None:intergenic
TGTCCACCATGATGCGGCCA+GGG 0.498285 7.1:+8465626 MS.gene22444:CDS
GCAAGCTTTGCTAGCTCTTG+AGG 0.501273 7.1:-8467149 None:intergenic
GCTATGGTTGGTAATTCTAG+TGG 0.507355 7.1:+8467086 MS.gene22444:CDS
GGTGGAAGAGGATAGTGAAA+AGG 0.509534 7.1:-8465209 None:intergenic
AGAGCAATGACTTCAGCATC+CGG 0.519151 7.1:-8465426 None:intergenic
TGACCATATTAACCTCATCA+TGG 0.523171 7.1:-8467007 None:intergenic
CAACAATTTAGTCCACATGC+AGG 0.543450 7.1:+8466714 MS.gene22444:CDS
TAAACTCGTTGTTTGTGTAG+AGG 0.545158 7.1:-8466976 None:intergenic
GGCCGCATCATGGTGGACAC+AGG 0.576015 7.1:-8465622 None:intergenic
AATCTTCAGCTTCATAGAAG+AGG 0.576550 7.1:+8465522 MS.gene22444:CDS
AGTAGTAGTGCAGCTATGGT+TGG 0.587266 7.1:+8467074 MS.gene22444:CDS
TATTCCGGTGAGGTCACCCA+AGG 0.589649 7.1:-8465649 None:intergenic
GGTGGACACAGGTCTTCTCC+GGG 0.591060 7.1:-8465611 None:intergenic
AGAAATCTACCAGGAACACC+AGG 0.591098 7.1:+8465298 MS.gene22444:CDS
TGCTCATGAAATCCTGCATG+TGG 0.593771 7.1:-8466726 None:intergenic
ACTGTGGTACACTCTTCTCT+AGG 0.600160 7.1:+8465799 MS.gene22444:CDS
TCACTATCCTCTTCCACCGG+AGG 0.603318 7.1:+8465214 MS.gene22444:CDS
GAAGAGGTCACAACCTACCA+TGG 0.609397 7.1:+8465538 MS.gene22444:CDS
TTTAAACCAAAACCATGATG+AGG 0.609478 7.1:+8466995 MS.gene22444:CDS
GTCTGCGAAATATGCAACAA+AGG 0.612364 7.1:+8465483 MS.gene22444:CDS
AGAAGTGCAGTAGCTGACAT+TGG 0.615729 7.1:-8466870 None:intergenic
TACCATGGAAGCTAAAGCAA+AGG 0.618651 7.1:+8465553 MS.gene22444:CDS
AATGTTTCTTTATTCCGGTG+AGG 0.626985 7.1:-8465659 None:intergenic
CATGGATTGATATCATCTCA+AGG 0.627308 7.1:+8466678 MS.gene22444:CDS
AAGTTTGTCAAAACCTCCGG+TGG 0.633133 7.1:-8465227 None:intergenic
GACCTGTGTCCACCATGATG+CGG 0.639865 7.1:+8465620 MS.gene22444:CDS
ACACAAACTAGTTTGTCACA+TGG 0.646370 7.1:+8466660 MS.gene22444:CDS
AAAGCTCACACTAAAACTTG+TGG 0.646859 7.1:+8465756 MS.gene22444:CDS
AAAGGTCCAACAAAGAACCA+AGG 0.658252 7.1:+8465571 MS.gene22444:CDS
AAGAAGAAGAGAAATCTACC+AGG 0.661464 7.1:+8465289 MS.gene22444:CDS
AGGCCCTGGCCGCATCATGG+TGG 0.664444 7.1:-8465629 None:intergenic
AAAGACCAAACTTGTCATTG+TGG 0.669911 7.1:+8466748 MS.gene22444:CDS
AGAGAATATAAGTGTGACTG+TGG 0.678389 7.1:+8465783 MS.gene22444:CDS
TGGAAGAGGATAGTGAAAAG+GGG 0.698551 7.1:-8465207 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTTATTTATTAAATATTAAA+AGG - chr7.1:8465345-8465364 None:intergenic 0.0%
!! ATAATAGCATTAAATTATAA+TGG + chr7.1:8466356-8466375 MS.gene22444:intron 10.0%
!!! TTATTAACAAATTTTTACAT+CGG + chr7.1:8465911-8465930 MS.gene22444:intron 10.0%
!! AAAGTTTAATTAATATTACC+TGG - chr7.1:8465319-8465338 None:intergenic 15.0%
!!! TTTCAAATATGTTTTGAATT+TGG + chr7.1:8465386-8465405 MS.gene22444:intron 15.0%
!!! TTTTCAATATCAAAAGATTT+TGG + chr7.1:8465854-8465873 MS.gene22444:intron 15.0%
!! AAAATAGTACTACAATTTGA+TGG - chr7.1:8466411-8466430 None:intergenic 20.0%
!! CAAATTAAGAAAAGTTGTTA+AGG + chr7.1:8466205-8466224 MS.gene22444:intron 20.0%
!! CAAGTTCAAATAAAAAGATA+TGG - chr7.1:8466292-8466311 None:intergenic 20.0%
!! TTGTGAATAATTAAATTCAG+AGG - chr7.1:8466188-8466207 None:intergenic 20.0%
!!! ACTATTTTTTTGCCTATATA+TGG + chr7.1:8466423-8466442 MS.gene22444:intron 20.0%
!!! TTTTTCTAATGTTATGCAAA+TGG + chr7.1:8466833-8466852 MS.gene22444:CDS 20.0%
! AATACTCCATAACCATATAT+AGG - chr7.1:8466438-8466457 None:intergenic 25.0%
! AGTTTAAATCAATTCAATGC+TGG + chr7.1:8467194-8467213 MS.gene22444:CDS 25.0%
! ATAAAAAGATATGGTAGTCT+AGG - chr7.1:8466283-8466302 None:intergenic 25.0%
! ATGAATTGCATGGTTAAAAT+TGG - chr7.1:8465993-8466012 None:intergenic 25.0%
! GCTAAAATGTTTCTTTATTC+CGG - chr7.1:8465667-8465686 None:intergenic 25.0%
! GTAAATGATTAGGTTTCTAA+TGG + chr7.1:8466502-8466521 MS.gene22444:intron 25.0%
! GTTTAAATCAATTCAATGCT+GGG + chr7.1:8467195-8467214 MS.gene22444:CDS 25.0%
!! ATAAAAACTTGTTTCGTTCA+AGG + chr7.1:8466313-8466332 MS.gene22444:intron 25.0%
!! ATGGTTAAAATTGGTTTAAC+TGG - chr7.1:8465984-8466003 None:intergenic 25.0%
!! TTTTTGCCTATATATGGTTA+TGG + chr7.1:8466429-8466448 MS.gene22444:intron 25.0%
!!! AAACATTTTAGCAGAAAACA+TGG + chr7.1:8465675-8465694 MS.gene22444:CDS 25.0%
!!! GATTAATCTGTTGATTTTCT+TGG - chr7.1:8466779-8466798 None:intergenic 25.0%
!!! TAAATCTTTTGTTTTCGATG+TGG + chr7.1:8466046-8466065 MS.gene22444:intron 25.0%
AAACCATGATGAGGTTAATA+TGG + chr7.1:8467004-8467023 MS.gene22444:CDS 30.0%
AAACTTCCACTAAAAATGGA+AGG - chr7.1:8466939-8466958 None:intergenic 30.0%
AATATGCAGTTCAATCTGAT+TGG + chr7.1:8465733-8465752 MS.gene22444:CDS 30.0%
ACCAAAACTTCCACTAAAAA+TGG - chr7.1:8466943-8466962 None:intergenic 30.0%
ACTGCATAGTGTAAATGATT+AGG + chr7.1:8466492-8466511 MS.gene22444:intron 30.0%
AGATTGAACTGCATATTTCT+TGG - chr7.1:8465730-8465749 None:intergenic 30.0%
ATCAGTATGAATGAATTGCA+TGG - chr7.1:8466003-8466022 None:intergenic 30.0%
TAGAATAAACAAGCACTACT+CGG - chr7.1:8466467-8466486 None:intergenic 30.0%
TAGTTTCAAAGACAAAAGTC+AGG + chr7.1:8465882-8465901 MS.gene22444:intron 30.0%
TTTAAACCAAAACCATGATG+AGG + chr7.1:8466995-8467014 MS.gene22444:CDS 30.0%
! TATTAACCTCATCATGGTTT+TGG - chr7.1:8467004-8467023 None:intergenic 30.0%
! TTCTGGAAATTTAGAGTTGT+AGG - chr7.1:8466615-8466634 None:intergenic 30.0%
!! ACTAATCCTTCCATTTTTAG+TGG + chr7.1:8466930-8466949 MS.gene22444:CDS 30.0%
!!! TCCATTTTTAGTGGAAGTTT+TGG + chr7.1:8466939-8466958 MS.gene22444:CDS 30.0%
AAAGACCAAACTTGTCATTG+TGG + chr7.1:8466748-8466767 MS.gene22444:CDS 35.0%
AAAGCTCACACTAAAACTTG+TGG + chr7.1:8465756-8465775 MS.gene22444:CDS 35.0%
AAGAAGAAGAGAAATCTACC+AGG + chr7.1:8465289-8465308 MS.gene22444:CDS 35.0%
AATCTTCAGCTTCATAGAAG+AGG + chr7.1:8465522-8465541 MS.gene22444:CDS 35.0%
AATGTTTCTTTATTCCGGTG+AGG - chr7.1:8465662-8465681 None:intergenic 35.0%
ACTCATCATCGATGAACTTA+TGG - chr7.1:8467177-8467196 None:intergenic 35.0%
AGAGAATATAAGTGTGACTG+TGG + chr7.1:8465783-8465802 MS.gene22444:CDS 35.0%
CAACAAAGAACCAAGGAAAA+AGG + chr7.1:8465578-8465597 MS.gene22444:CDS 35.0%
GAAAACATGGTGAGAAGAAA+TGG + chr7.1:8465688-8465707 MS.gene22444:CDS 35.0%
GGATTGAAATACTACAGATC+CGG + chr7.1:8465407-8465426 MS.gene22444:intron 35.0%
TAAACTCGTTGTTTGTGTAG+AGG - chr7.1:8466979-8466998 None:intergenic 35.0%
TAGATTTCTCTTCTTCTTGC+TGG - chr7.1:8465287-8465306 None:intergenic 35.0%
TATTTGACGACTCTTCATTC+TGG - chr7.1:8466632-8466651 None:intergenic 35.0%
TCTTTCTCCAAAGACTCTAA+TGG + chr7.1:8465446-8465465 MS.gene22444:CDS 35.0%
TGACCATATTAACCTCATCA+TGG - chr7.1:8467010-8467029 None:intergenic 35.0%
TTAATATTACCTGGTGTTCC+TGG - chr7.1:8465310-8465329 None:intergenic 35.0%
TTCAACCACAATGACAAGTT+TGG - chr7.1:8466756-8466775 None:intergenic 35.0%
TTGAAGTTTGTCAAAACCTC+CGG - chr7.1:8465233-8465252 None:intergenic 35.0%
! AATGGTACATTTTACTGCAC+AGG + chr7.1:8466520-8466539 MS.gene22444:intron 35.0%
! ACACAAACTAGTTTGTCACA+TGG + chr7.1:8466660-8466679 MS.gene22444:CDS 35.0%
! CAGATGTATACCTTTTTCCT+TGG - chr7.1:8465591-8465610 None:intergenic 35.0%
! CATGGATTGATATCATCTCA+AGG + chr7.1:8466678-8466697 MS.gene22444:CDS 35.0%
!! AGTGGGTTTAGAGATTTTCT+TGG + chr7.1:8467104-8467123 MS.gene22444:CDS 35.0%
!! CTATGGTTGGTAATTCTAGT+GGG + chr7.1:8467087-8467106 MS.gene22444:CDS 35.0%
!! CTTTTTCCTTGGTTCTTTGT+TGG - chr7.1:8465580-8465599 None:intergenic 35.0%
AAAGGTCCAACAAAGAACCA+AGG + chr7.1:8465571-8465590 MS.gene22444:CDS 40.0%
CAACAATTTAGTCCACATGC+AGG + chr7.1:8466714-8466733 MS.gene22444:CDS 40.0%
CTTCAGAAAGCAGCTCAAAT+TGG + chr7.1:8466891-8466910 MS.gene22444:CDS 40.0%
GCTCTTGAGGTAGAAATTGA+TGG - chr7.1:8467139-8467158 None:intergenic 40.0%
GCTGAAGATTCTGATCTCTT+TGG - chr7.1:8465512-8465531 None:intergenic 40.0%
GTCTGCGAAATATGCAACAA+AGG + chr7.1:8465483-8465502 MS.gene22444:CDS 40.0%
GTGGAAGAGGATAGTGAAAA+GGG - chr7.1:8465211-8465230 None:intergenic 40.0%
TACCATGGAAGCTAAAGCAA+AGG + chr7.1:8465553-8465572 MS.gene22444:CDS 40.0%
TAGTAGTAGTAGTGCAGCTA+TGG + chr7.1:8467070-8467089 MS.gene22444:CDS 40.0%
TGCATATTTCGCAGACGAAT+CGG - chr7.1:8465479-8465498 None:intergenic 40.0%
TGGAAGAGGATAGTGAAAAG+GGG - chr7.1:8465210-8465229 None:intergenic 40.0%
! GAAAAAGGTATACATCTGCC+CGG + chr7.1:8465593-8465612 MS.gene22444:CDS 40.0%
! GTACATTTTACTGCACAGGA+AGG + chr7.1:8466524-8466543 MS.gene22444:intron 40.0%
! TTTGCTTTAGCTTCCATGGT+AGG - chr7.1:8465554-8465573 None:intergenic 40.0%
! TTTTCACTATCCTCTTCCAC+CGG + chr7.1:8465211-8465230 MS.gene22444:CDS 40.0%
!! GCTATGGTTGGTAATTCTAG+TGG + chr7.1:8467086-8467105 MS.gene22444:CDS 40.0%
!! TTTGTGGTGGATTAGGGTTA+GGG - chr7.1:8465264-8465283 None:intergenic 40.0%
AAGTTTGTCAAAACCTCCGG+TGG - chr7.1:8465230-8465249 None:intergenic 45.0%
ACTGTGGTACACTCTTCTCT+AGG + chr7.1:8465799-8465818 MS.gene22444:CDS 45.0%
AGAAATCTACCAGGAACACC+AGG + chr7.1:8465298-8465317 MS.gene22444:CDS 45.0%
AGAGCAATGACTTCAGCATC+CGG - chr7.1:8465429-8465448 None:intergenic 45.0%
AGTAGTAGTGCAGCTATGGT+TGG + chr7.1:8467074-8467093 MS.gene22444:CDS 45.0%
GCTGCACTACTACTACTACT+TGG - chr7.1:8467068-8467087 None:intergenic 45.0%
GGTGGAAGAGGATAGTGAAA+AGG - chr7.1:8465212-8465231 None:intergenic 45.0%
TGCTCATGAAATCCTGCATG+TGG - chr7.1:8466729-8466748 None:intergenic 45.0%
TTCGTCGCCATTAGAGTCTT+TGG - chr7.1:8465456-8465475 None:intergenic 45.0%
! AGAAGTGCAGTAGCTGACAT+TGG - chr7.1:8466873-8466892 None:intergenic 45.0%
! GACCTTTGCTTTAGCTTCCA+TGG - chr7.1:8465558-8465577 None:intergenic 45.0%
!! CTCTTCTTCTTGCTGGTTTG+TGG - chr7.1:8465280-8465299 None:intergenic 45.0%
!! GTTTGTGGTGGATTAGGGTT+AGG - chr7.1:8465265-8465284 None:intergenic 45.0%
!! TGCTGGTTTGTGGTGGATTA+GGG - chr7.1:8465270-8465289 None:intergenic 45.0%
!! TTCTTCTTGCTGGTTTGTGG+TGG - chr7.1:8465277-8465296 None:intergenic 45.0%
!! TTGCTGGTTTGTGGTGGATT+AGG - chr7.1:8465271-8465290 None:intergenic 45.0%
GAAGAGGTCACAACCTACCA+TGG + chr7.1:8465538-8465557 MS.gene22444:CDS 50.0%
! GCAAGCTTTGCTAGCTCTTG+AGG - chr7.1:8467152-8467171 None:intergenic 50.0%
GACCTGTGTCCACCATGATG+CGG + chr7.1:8465620-8465639 MS.gene22444:CDS 55.0%
GTCAAAACCTCCGGTGGAAG+AGG - chr7.1:8465224-8465243 None:intergenic 55.0%
TATTCCGGTGAGGTCACCCA+AGG - chr7.1:8465652-8465671 None:intergenic 55.0%
TCACTATCCTCTTCCACCGG+AGG + chr7.1:8465214-8465233 MS.gene22444:CDS 55.0%
! TGGTGGACACAGGTCTTCTC+CGG - chr7.1:8465615-8465634 None:intergenic 55.0%
GGTGGACACAGGTCTTCTCC+GGG - chr7.1:8465614-8465633 None:intergenic 60.0%
TGTCCACCATGATGCGGCCA+GGG + chr7.1:8465626-8465645 MS.gene22444:CDS 60.0%
AGGGCCTTGGGTGACCTCAC+CGG + chr7.1:8465645-8465664 MS.gene22444:CDS 65.0%
CATGATGCGGCCAGGGCCTT+GGG + chr7.1:8465633-8465652 MS.gene22444:CDS 65.0%
GTGTCCACCATGATGCGGCC+AGG + chr7.1:8465625-8465644 MS.gene22444:CDS 65.0%
!! GGCCGCATCATGGTGGACAC+AGG - chr7.1:8465625-8465644 None:intergenic 65.0%
AGGCCCTGGCCGCATCATGG+TGG - chr7.1:8465632-8465651 None:intergenic 70.0%
CCAAGGCCCTGGCCGCATCA+TGG - chr7.1:8465635-8465654 None:intergenic 70.0%
CCATGATGCGGCCAGGGCCT+TGG + chr7.1:8465632-8465651 MS.gene22444:CDS 70.0%
GGTGAGGTCACCCAAGGCCC+TGG - chr7.1:8465646-8465665 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 8465190 8467235 8465190 ID=MS.gene22444
chr7.1 mRNA 8465190 8467235 8465190 ID=MS.gene22444.t1;Parent=MS.gene22444
chr7.1 exon 8465190 8465319 8465190 ID=MS.gene22444.t1.exon1;Parent=MS.gene22444.t1
chr7.1 CDS 8465190 8465319 8465190 ID=cds.MS.gene22444.t1;Parent=MS.gene22444.t1
chr7.1 exon 8465424 8465820 8465424 ID=MS.gene22444.t1.exon2;Parent=MS.gene22444.t1
chr7.1 CDS 8465424 8465820 8465424 ID=cds.MS.gene22444.t1;Parent=MS.gene22444.t1
chr7.1 exon 8466542 8467235 8466542 ID=MS.gene22444.t1.exon3;Parent=MS.gene22444.t1
chr7.1 CDS 8466542 8467235 8466542 ID=cds.MS.gene22444.t1;Parent=MS.gene22444.t1
Gene Sequence

>MS.gene22444

ATGAATATTCTTGGTGACCCCTTTTCACTATCCTCTTCCACCGGAGGTTTTGACAAACTTCAAAACACAAACCCTAACCCTAATCCACCACAAACCAGCAAGAAGAAGAGAAATCTACCAGGAACACCAGGTAATATTAATTAAACTTTTTTCCTTTTAATATTTAATAAATAAAGTATTTTTGCTATATATATTTTTTCAAATATGTTTTGAATTTGGATTGAAATACTACAGATCCGGATGCTGAAGTCATTGCTCTTTCTCCAAAGACTCTAATGGCGACGAACCGATTCGTCTGCGAAATATGCAACAAAGGATTCCAAAGAGATCAGAATCTTCAGCTTCATAGAAGAGGTCACAACCTACCATGGAAGCTAAAGCAAAGGTCCAACAAAGAACCAAGGAAAAAGGTATACATCTGCCCGGAGAAGACCTGTGTCCACCATGATGCGGCCAGGGCCTTGGGTGACCTCACCGGAATAAAGAAACATTTTAGCAGAAAACATGGTGAGAAGAAATGGAAGTGTGATAAGTGTTCCAAGAAATATGCAGTTCAATCTGATTGGAAAGCTCACACTAAAACTTGTGGTACTAGAGAATATAAGTGTGACTGTGGTACACTCTTCTCTAGGTAATTTAATTCATTAATTTTTTTCATTTTTAATTTTCAATATCAAAAGATTTTGGTGACTTAGTTTCAAAGACAAAAGTCAGGACAAATTTATTAACAAATTTTTACATCGGCAGTCAAATTTAAACTTACGTTCTTGCAAGATATGAGTGTATTATTACCAGTTAAACCAATTTTAACCATGCAATTCATTCATACTGATTTTTTATGAACAAAAAGTTTCTGTAAATCTTTTGTTTTCGATGTGGATGAAAAAACTGTTTTTTTTTTTTTTTTTACAAGTCAAAATAAAAAGCTATTTTTCTTTTCATTTAGTTATACTTCTATCATGCATTTGCATTATGTTTGAATTTAATGCATACTTCCTCTGAATTTAATTATTCACAAATTAAGAAAAGTTGTTAAGGTATATCTCAAACTATAATGTTTTGTTGACTTAAAAATGTTACTATTGCATTTCCTAGACTACCATATCTTTTTATTTGAACTTGTATAAAAACTTGTTTCGTTCAAGGATATTTTGATAATAATAATAATAATAGCATTAAATTATAATGGTGAAAGTAGTTCGATTTTACTTATATTGTCCATCAAATTGTAGTACTATTTTTTTGCCTATATATGGTTATGGAGTATTTAAATTCCGAGTAGTGCTTGTTTATTCTAGTACTACTGCATAGTGTAAATGATTAGGTTTCTAATGGTACATTTTACTGCACAGGAAGGATAGCTTTATTACACATAGAGCTTTCTGTGACGCATTAGCTGTAGAAAGTGCAAGACACTCATCTCCTACAACTCTAAATTTCCAGAATGAAGAGTCGTCAAATATGATGAACACACAAACTAGTTTGTCACATGGATTGATATCATCTCAAGGACTTCAAAGTCTTCAACAATTTAGTCCACATGCAGGATTTCATGAGCAAAGACCAAACTTGTCATTGTGGTTGAACCAAGAAAATCAACAGATTAATCATCATTCACATTCAATTGATCATGTTTCATCAGATTTTTCTAATGTTATGCAAATGGCTCAAACTAATACTCCAATGTCAGCTACTGCACTTCTTCAGAAAGCAGCTCAAATTGGTTCAACAAGAAGTACTACTAATCCTTCCATTTTTAGTGGAAGTTTTGGTGTAATGAGTTCATCCTCTACACAAACAACGAGTTTAAACCAAAACCATGATGAGGTTAATATGGTCATTAATCAGAAAATGAAGCAGCATGAGAATTTTGATCCAAGTAGTAGTAGTAGTGCAGCTATGGTTGGTAATTCTAGTGGGTTTAGAGATTTTCTTGGAGTGAGTAACCATCAATTTCTACCTCAAGAGCTAGCAAAGCTTGCTTCCATAAGTTCATCGATGATGAGTTTAAATCAATTCAATGCTGGGTGCATCAATGAAAATTAA
Protein sequence

>MS.gene22444.t1

MNILGDPFSLSSSTGGFDKLQNTNPNPNPPQTSKKKRNLPGTPDPDAEVIALSPKTLMATNRFVCEICNKGFQRDQNLQLHRRGHNLPWKLKQRSNKEPRKKVYICPEKTCVHHDAARALGDLTGIKKHFSRKHGEKKWKCDKCSKKYAVQSDWKAHTKTCGTREYKCDCGTLFSRKDSFITHRAFCDALAVESARHSSPTTLNFQNEESSNMMNTQTSLSHGLISSQGLQSLQQFSPHAGFHEQRPNLSLWLNQENQQINHHSHSIDHVSSDFSNVMQMAQTNTPMSATALLQKAAQIGSTRSTTNPSIFSGSFGVMSSSSTQTTSLNQNHDEVNMVINQKMKQHENFDPSSSSSAAMVGNSSGFRDFLGVSNHQFLPQELAKLASISSSMMSLNQFNAGCINEN