Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22652.t1 | AES71801.2 | 45.8 | 96 | 38 | 1 | 30 | 125 | 327 | 408 | 6.90E-13 | 84.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22652.t1 | G7J8X0 | 45.8 | 96 | 38 | 1 | 30 | 125 | 327 | 408 | 5.0e-13 | 84.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene22652.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22652.t1 | MTR_3g082920 | 45.833 | 96 | 38 | 1 | 30 | 125 | 327 | 408 | 3.80e-18 | 82.0 |
| MS.gene22652.t1 | MTR_6g477950 | 48.000 | 75 | 39 | 0 | 125 | 199 | 70 | 144 | 1.09e-14 | 71.6 |
| MS.gene22652.t1 | MTR_8g069780 | 38.000 | 100 | 56 | 1 | 100 | 199 | 62 | 155 | 2.09e-13 | 65.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 52 sgRNAs with CRISPR-Local
Find 89 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACCAGAAGTCGTGAAATTT+TGG | 0.193946 | 3.3:+43349890 | None:intergenic |
| GCAAACTTATCACTTCAAAT+AGG | 0.238418 | 3.3:-43349432 | MS.gene22652:CDS |
| AGCCAAAATTTCACGACTTC+TGG | 0.271151 | 3.3:-43349892 | MS.gene22652:CDS |
| TCACCTAAGTATTTCTCATT+TGG | 0.308885 | 3.3:+43349406 | None:intergenic |
| AAACTGCAAAAGTATGATTA+TGG | 0.310821 | 3.3:-43349970 | MS.gene22652:CDS |
| CAAACTTATCACTTCAAATA+GGG | 0.313955 | 3.3:-43349431 | MS.gene22652:CDS |
| ACCTGTGTACGAGTTAAGTT+TGG | 0.331180 | 3.3:-43349925 | MS.gene22652:CDS |
| CAGGTTCAAGATGATGGTTT+TGG | 0.363392 | 3.3:-43349797 | MS.gene22652:CDS |
| AGTATGATTATGGATGGATT+AGG | 0.381484 | 3.3:-43349960 | MS.gene22652:CDS |
| TCTACTATCCTTCACGAACT+TGG | 0.384344 | 3.3:+43349375 | None:intergenic |
| CGCTTTCCAATGGAATCAAC+CGG | 0.384860 | 3.3:-43350673 | None:intergenic |
| ACAGGAGAGCATTACTTTGC+AGG | 0.396717 | 3.3:-43349482 | MS.gene22652:CDS |
| ACGTCGCAGCTAAATTCAAC+TGG | 0.409112 | 3.3:-43349864 | MS.gene22652:CDS |
| ACTCGTACACAGGTGCCATT+TGG | 0.409607 | 3.3:+43349934 | None:intergenic |
| TAAGTTTGCACTCATATTCT+CGG | 0.417716 | 3.3:+43349446 | None:intergenic |
| AACTATCATTTATCCACAAC+AGG | 0.425989 | 3.3:-43350151 | MS.gene22652:intron |
| GTATGATTATGGATGGATTA+GGG | 0.435600 | 3.3:-43349959 | MS.gene22652:CDS |
| GGTTGTCCGACGTCGTCGTT+AGG | 0.436504 | 3.3:-43349290 | None:intergenic |
| ACCTCCACAAAGAGACGATT+GGG | 0.451863 | 3.3:+43349310 | None:intergenic |
| AGAAATACTTAGGTGAAGAT+TGG | 0.462766 | 3.3:-43349399 | MS.gene22652:CDS |
| TCCTCCCAATCGTCTCTTTG+TGG | 0.465471 | 3.3:-43349314 | MS.gene22652:CDS |
| GTAATGCTCTCCTGTGTGTC+GGG | 0.473972 | 3.3:+43349490 | None:intergenic |
| CAATGGAATCAACCGGGAAA+AGG | 0.480353 | 3.3:-43350666 | MS.gene22652:intron |
| TGGATGAAGTTGGAAGTTCA+AGG | 0.497813 | 3.3:-43349774 | MS.gene22652:CDS |
| CGTGTTAGACTTGAATATAC+TGG | 0.498920 | 3.3:-43349829 | MS.gene22652:CDS |
| CCTGTGTACGAGTTAAGTTT+GGG | 0.512909 | 3.3:-43349924 | MS.gene22652:CDS |
| GGTCCAAATGAGAAATACTT+AGG | 0.515401 | 3.3:-43349409 | MS.gene22652:CDS |
| TGCAAAAGTATGATTATGGA+TGG | 0.520237 | 3.3:-43349966 | MS.gene22652:CDS |
| TGGATAAATGATAGTTTCGA+AGG | 0.529484 | 3.3:+43350158 | None:intergenic |
| CTACTATCCTTCACGAACTT+GGG | 0.542504 | 3.3:+43349376 | None:intergenic |
| AACCTCCACAAAGAGACGAT+TGG | 0.547571 | 3.3:+43349309 | None:intergenic |
| TATTCAAGTCTAACACGTGT+TGG | 0.547805 | 3.3:+43349835 | None:intergenic |
| AAATTTCACGACTTCTGGTA+GGG | 0.551367 | 3.3:-43349887 | MS.gene22652:CDS |
| TTCAAGGGGAAATACAATCA+AGG | 0.560197 | 3.3:-43349758 | MS.gene22652:CDS |
| TCCCAATCGTCTCTTTGTGG+AGG | 0.560244 | 3.3:-43349311 | MS.gene22652:CDS |
| GGATGAAGTTGGAAGTTCAA+GGG | 0.560255 | 3.3:-43349773 | MS.gene22652:CDS |
| AGTAATGCTCTCCTGTGTGT+CGG | 0.561176 | 3.3:+43349489 | None:intergenic |
| TCGTGAAGGATAGTAGACTA+AGG | 0.562838 | 3.3:-43349369 | MS.gene22652:CDS |
| GGATGGATTAGGGATCCAAA+TGG | 0.577469 | 3.3:-43349949 | MS.gene22652:CDS |
| CCCAAACTTAACTCGTACAC+AGG | 0.584015 | 3.3:+43349924 | None:intergenic |
| AAAATTTCACGACTTCTGGT+AGG | 0.588749 | 3.3:-43349888 | MS.gene22652:CDS |
| AAACTTATCACTTCAAATAG+GGG | 0.599416 | 3.3:-43349430 | MS.gene22652:CDS |
| GATGAAGTTGGAAGTTCAAG+GGG | 0.628048 | 3.3:-43349772 | MS.gene22652:CDS |
| CGTCGCAGCTAAATTCAACT+GGG | 0.629434 | 3.3:-43349863 | MS.gene22652:CDS |
| AAGGATAGTAGACTAAGGAA+AGG | 0.649294 | 3.3:-43349364 | MS.gene22652:CDS |
| CAACTGCTTACCCGACACAC+AGG | 0.661269 | 3.3:-43349500 | MS.gene22652:CDS |
| TCCACAAAGAGACGATTGGG+AGG | 0.661851 | 3.3:+43349313 | None:intergenic |
| GCTTTCCAATGGAATCAACC+GGG | 0.669471 | 3.3:-43350672 | None:intergenic |
| TAATATCCTAACGACGACGT+CGG | 0.679159 | 3.3:+43349284 | None:intergenic |
| AGATTGGCCCAAGTTCGTGA+AGG | 0.685876 | 3.3:-43349383 | MS.gene22652:CDS |
| TAGTTTCGAAGGAATTCACG+AGG | 0.693574 | 3.3:+43350169 | None:intergenic |
| AGTTTCGAAGGAATTCACGA+GGG | 0.715405 | 3.3:+43350170 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTCATAAATAATATTAATT+TGG | + | chr3.3:43350346-43350365 | None:intergenic | 10.0% |
| !!! | TTTTTATTATATTCTTTCTA+TGG | + | chr3.3:43349672-43349691 | None:intergenic | 10.0% |
| !! | ATACATATTTATGATTTAAC+AGG | - | chr3.3:43349595-43349614 | MS.gene22652:intron | 15.0% |
| !! | TTTCAGTAACAAAAAAAATT+TGG | + | chr3.3:43349498-43349517 | None:intergenic | 15.0% |
| !!! | ATATTTTATCAAATTTCGTA+TGG | - | chr3.3:43349871-43349890 | MS.gene22652:CDS | 15.0% |
| !! | TAAAACATAGAAGGTAATAT+GGG | - | chr3.3:43349411-43349430 | MS.gene22652:CDS | 20.0% |
| ! | AAACTTATCACTTCAAATAG+GGG | - | chr3.3:43350525-43350544 | MS.gene22652:intron | 25.0% |
| ! | ACATATAAGATGTATAAGTC+AGG | + | chr3.3:43349899-43349918 | None:intergenic | 25.0% |
| ! | AGGTATTACAAAATATTGCT+TGG | - | chr3.3:43349824-43349843 | MS.gene22652:CDS | 25.0% |
| ! | ATATAAGATGTATAAGTCAG+GGG | + | chr3.3:43349897-43349916 | None:intergenic | 25.0% |
| ! | CAAACTTATCACTTCAAATA+GGG | - | chr3.3:43350524-43350543 | MS.gene22652:intron | 25.0% |
| ! | CACTATTACTATATTTACGA+TGG | + | chr3.3:43349364-43349383 | None:intergenic | 25.0% |
| ! | CATATAAGATGTATAAGTCA+GGG | + | chr3.3:43349898-43349917 | None:intergenic | 25.0% |
| ! | CATCGTAAATATAGTAATAG+TGG | - | chr3.3:43349362-43349381 | MS.gene22652:CDS | 25.0% |
| ! | CTAAAACATAGAAGGTAATA+TGG | - | chr3.3:43349410-43349429 | MS.gene22652:CDS | 25.0% |
| !! | AAACTGCAAAAGTATGATTA+TGG | - | chr3.3:43349985-43350004 | MS.gene22652:CDS | 25.0% |
| !! | ATATTTTGTAATACCTGTTG+TGG | + | chr3.3:43349820-43349839 | None:intergenic | 25.0% |
| !! | TTTTTACATATCCCATGTTA+TGG | - | chr3.3:43349633-43349652 | MS.gene22652:intron | 25.0% |
| !!! | CAGTTGATAGATATCTTATT+GGG | + | chr3.3:43350441-43350460 | None:intergenic | 25.0% |
| AACTATCATTTATCCACAAC+AGG | - | chr3.3:43349804-43349823 | MS.gene22652:CDS | 30.0% | |
| AGAAATACTTAGGTGAAGAT+TGG | - | chr3.3:43350556-43350575 | MS.gene22652:intron | 30.0% | |
| ATATCCCATGTTATGGAATT+CGG | - | chr3.3:43349640-43349659 | MS.gene22652:intron | 30.0% | |
| CACATATCCTTATCAAGTTT+AGG | - | chr3.3:43349520-43349539 | MS.gene22652:intron | 30.0% | |
| CTAAACTTGATAAGGATATG+TGG | + | chr3.3:43349522-43349541 | None:intergenic | 30.0% | |
| GCAAACTTATCACTTCAAAT+AGG | - | chr3.3:43350523-43350542 | MS.gene22652:intron | 30.0% | |
| TAAGTTTGCACTCATATTCT+CGG | + | chr3.3:43350512-43350531 | None:intergenic | 30.0% | |
| TCACCTAAGTATTTCTCATT+TGG | + | chr3.3:43350552-43350571 | None:intergenic | 30.0% | |
| TGGATAAATGATAGTTTCGA+AGG | + | chr3.3:43349800-43349819 | None:intergenic | 30.0% | |
| ! | ACATGCAATTTTGTAATGAG+TGG | - | chr3.3:43349555-43349574 | MS.gene22652:intron | 30.0% |
| ! | AGAGTAAAATTACCTTTTCC+CGG | + | chr3.3:43349304-43349323 | None:intergenic | 30.0% |
| ! | AGTATGATTATGGATGGATT+AGG | - | chr3.3:43349995-43350014 | MS.gene22652:CDS | 30.0% |
| ! | CTGATTATGATCATTTTGAG+AGG | - | chr3.3:43350265-43350284 | MS.gene22652:intron | 30.0% |
| ! | GTAATATGGGTTCTTTTCTA+GGG | - | chr3.3:43349424-43349443 | MS.gene22652:CDS | 30.0% |
| ! | TGCAAAAGTATGATTATGGA+TGG | - | chr3.3:43349989-43350008 | MS.gene22652:CDS | 30.0% |
| ! | TTTTAACAGGTTCAAGATGA+TGG | - | chr3.3:43350152-43350171 | MS.gene22652:CDS | 30.0% |
| !! | GCAGTTGATAGATATCTTAT+TGG | + | chr3.3:43350442-43350461 | None:intergenic | 30.0% |
| !! | GTATGATTATGGATGGATTA+GGG | - | chr3.3:43349996-43350015 | MS.gene22652:CDS | 30.0% |
| AAAATTTCACGACTTCTGGT+AGG | - | chr3.3:43350067-43350086 | MS.gene22652:intron | 35.0% | |
| AAATTTCACGACTTCTGGTA+GGG | - | chr3.3:43350068-43350087 | MS.gene22652:intron | 35.0% | |
| AAGGATAGTAGACTAAGGAA+AGG | - | chr3.3:43350591-43350610 | MS.gene22652:intron | 35.0% | |
| CAACAAGAGATGTCAAGTAA+TGG | - | chr3.3:43350311-43350330 | MS.gene22652:intron | 35.0% | |
| CGTGTTAGACTTGAATATAC+TGG | - | chr3.3:43350126-43350145 | MS.gene22652:intron | 35.0% | |
| GGTCCAAATGAGAAATACTT+AGG | - | chr3.3:43350546-43350565 | MS.gene22652:intron | 35.0% | |
| TAAGGTGGCTAAAACATAGA+AGG | - | chr3.3:43349402-43349421 | MS.gene22652:CDS | 35.0% | |
| TATTCAAGTCTAACACGTGT+TGG | + | chr3.3:43350123-43350142 | None:intergenic | 35.0% | |
| TTCAAGGGGAAATACAATCA+AGG | - | chr3.3:43350197-43350216 | MS.gene22652:CDS | 35.0% | |
| ! | AATATACTGGCGCTTTTAAC+AGG | - | chr3.3:43350139-43350158 | MS.gene22652:intron | 35.0% |
| ! | GGTAATATGGGTTCTTTTCT+AGG | - | chr3.3:43349423-43349442 | MS.gene22652:CDS | 35.0% |
| ! | TACCAGAAGTCGTGAAATTT+TGG | + | chr3.3:43350068-43350087 | None:intergenic | 35.0% |
| ACCTGTGTACGAGTTAAGTT+TGG | - | chr3.3:43350030-43350049 | MS.gene22652:intron | 40.0% | |
| AGCCAAAATTTCACGACTTC+TGG | - | chr3.3:43350063-43350082 | MS.gene22652:intron | 40.0% | |
| AGTTTCGAAGGAATTCACGA+GGG | + | chr3.3:43349788-43349807 | None:intergenic | 40.0% | |
| CCTGTGTACGAGTTAAGTTT+GGG | - | chr3.3:43350031-43350050 | MS.gene22652:intron | 40.0% | |
| CCTTATCAAGTTTAGGCTGA+CGG | - | chr3.3:43349527-43349546 | MS.gene22652:intron | 40.0% | |
| CTACTATCCTTCACGAACTT+GGG | + | chr3.3:43350582-43350601 | None:intergenic | 40.0% | |
| GATGAAGTTGGAAGTTCAAG+GGG | - | chr3.3:43350183-43350202 | MS.gene22652:CDS | 40.0% | |
| GGATGAAGTTGGAAGTTCAA+GGG | - | chr3.3:43350182-43350201 | MS.gene22652:CDS | 40.0% | |
| TAGTTTCGAAGGAATTCACG+AGG | + | chr3.3:43349789-43349808 | None:intergenic | 40.0% | |
| TCCACCGAATTCCATAACAT+GGG | + | chr3.3:43349647-43349666 | None:intergenic | 40.0% | |
| TCGTGAAGGATAGTAGACTA+AGG | - | chr3.3:43350586-43350605 | MS.gene22652:intron | 40.0% | |
| TCTACTATCCTTCACGAACT+TGG | + | chr3.3:43350583-43350602 | None:intergenic | 40.0% | |
| TGGATGAAGTTGGAAGTTCA+AGG | - | chr3.3:43350181-43350200 | MS.gene22652:CDS | 40.0% | |
| TTCCACCGAATTCCATAACA+TGG | + | chr3.3:43349648-43349667 | None:intergenic | 40.0% | |
| !! | CAGGTTCAAGATGATGGTTT+TGG | - | chr3.3:43350158-43350177 | MS.gene22652:CDS | 40.0% |
| !!! | ATGGTTTTGGTGGATGAAGT+TGG | - | chr3.3:43350171-43350190 | MS.gene22652:CDS | 40.0% |
| !!! | GTTCAAGATGATGGTTTTGG+TGG | - | chr3.3:43350161-43350180 | MS.gene22652:CDS | 40.0% |
| AACCTCCACAAAGAGACGAT+TGG | + | chr3.3:43350649-43350668 | None:intergenic | 45.0% | |
| ACCTCCACAAAGAGACGATT+GGG | + | chr3.3:43350648-43350667 | None:intergenic | 45.0% | |
| ACGTCGCAGCTAAATTCAAC+TGG | - | chr3.3:43350091-43350110 | MS.gene22652:intron | 45.0% | |
| AGTAATGCTCTCCTGTGTGT+CGG | + | chr3.3:43350469-43350488 | None:intergenic | 45.0% | |
| ATAGTGGTCCCCTATAGTCT+TGG | - | chr3.3:43349378-43349397 | MS.gene22652:CDS | 45.0% | |
| CACCTTAGCCAAGACTATAG+GGG | + | chr3.3:43349389-43349408 | None:intergenic | 45.0% | |
| CCACCTTAGCCAAGACTATA+GGG | + | chr3.3:43349390-43349409 | None:intergenic | 45.0% | |
| CCCAAACTTAACTCGTACAC+AGG | + | chr3.3:43350034-43350053 | None:intergenic | 45.0% | |
| CCGTCAGCCTAAACTTGATA+AGG | + | chr3.3:43349530-43349549 | None:intergenic | 45.0% | |
| CGTCGCAGCTAAATTCAACT+GGG | - | chr3.3:43350092-43350111 | MS.gene22652:intron | 45.0% | |
| TCCCATGTTATGGAATTCGG+TGG | - | chr3.3:43349643-43349662 | MS.gene22652:intron | 45.0% | |
| !! | ACAGGAGAGCATTACTTTGC+AGG | - | chr3.3:43350473-43350492 | MS.gene22652:intron | 45.0% |
| !! | GGATGGATTAGGGATCCAAA+TGG | - | chr3.3:43350006-43350025 | MS.gene22652:CDS | 45.0% |
| GCCACCTTAGCCAAGACTAT+AGG | + | chr3.3:43349391-43349410 | None:intergenic | 50.0% | |
| GTAATGCTCTCCTGTGTGTC+GGG | + | chr3.3:43350468-43350487 | None:intergenic | 50.0% | |
| TCCACAAAGAGACGATTGGG+AGG | + | chr3.3:43350645-43350664 | None:intergenic | 50.0% | |
| TCCCAATCGTCTCTTTGTGG+AGG | - | chr3.3:43350644-43350663 | MS.gene22652:intron | 50.0% | |
| TCCTCCCAATCGTCTCTTTG+TGG | - | chr3.3:43350641-43350660 | MS.gene22652:intron | 50.0% | |
| ! | AGATTGGCCCAAGTTCGTGA+AGG | - | chr3.3:43350572-43350591 | MS.gene22652:intron | 50.0% |
| ! | CCCTATAGTCTTGGCTAAGG+TGG | - | chr3.3:43349387-43349406 | MS.gene22652:CDS | 50.0% |
| ! | GTCCCCTATAGTCTTGGCTA+AGG | - | chr3.3:43349384-43349403 | MS.gene22652:CDS | 50.0% |
| !! | ACTCGTACACAGGTGCCATT+TGG | + | chr3.3:43350024-43350043 | None:intergenic | 50.0% |
| CAACTGCTTACCCGACACAC+AGG | - | chr3.3:43350455-43350474 | MS.gene22652:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 43349291 | 43350686 | 43349291 | ID=MS.gene22652 |
| chr3.3 | mRNA | 43349291 | 43350686 | 43349291 | ID=MS.gene22652.t1;Parent=MS.gene22652 |
| chr3.3 | exon | 43350667 | 43350686 | 43350667 | ID=MS.gene22652.t1.exon1;Parent=MS.gene22652.t1 |
| chr3.3 | CDS | 43350667 | 43350686 | 43350667 | ID=cds.MS.gene22652.t1;Parent=MS.gene22652.t1 |
| chr3.3 | exon | 43350152 | 43350218 | 43350152 | ID=MS.gene22652.t1.exon2;Parent=MS.gene22652.t1 |
| chr3.3 | CDS | 43350152 | 43350218 | 43350152 | ID=cds.MS.gene22652.t1;Parent=MS.gene22652.t1 |
| chr3.3 | exon | 43349753 | 43350037 | 43349753 | ID=MS.gene22652.t1.exon3;Parent=MS.gene22652.t1 |
| chr3.3 | CDS | 43349753 | 43350037 | 43349753 | ID=cds.MS.gene22652.t1;Parent=MS.gene22652.t1 |
| chr3.3 | exon | 43349291 | 43349530 | 43349291 | ID=MS.gene22652.t1.exon4;Parent=MS.gene22652.t1 |
| chr3.3 | CDS | 43349291 | 43349530 | 43349291 | ID=cds.MS.gene22652.t1;Parent=MS.gene22652.t1 |
| Gene Sequence |
| Protein sequence |
