AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene22816

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22816.t1 MTR_7g117705 67.412 313 77 4 1 291 1 310 3.00e-134 388
MS.gene22816.t1 MTR_1g013170 76.448 259 61 0 1 259 1 259 1.19e-126 370
MS.gene22816.t1 MTR_1g013180 63.077 325 86 3 1 291 152 476 5.74e-120 359
MS.gene22816.t1 MTR_3g106220 55.063 316 112 4 2 288 5 319 3.11e-100 307
MS.gene22816.t1 MTR_2g450070 51.429 280 123 5 3 272 16 292 2.16e-81 258
MS.gene22816.t1 MTR_2g450070 51.429 280 123 5 3 272 16 292 3.46e-81 258
MS.gene22816.t1 MTR_1g032570 48.315 267 121 3 4 260 10 269 8.74e-76 243
MS.gene22816.t1 MTR_4g121020 48.507 268 132 2 2 267 28 291 7.20e-75 242
MS.gene22816.t1 MTR_2g034960 47.203 286 145 3 1 286 25 304 7.48e-75 240
MS.gene22816.t1 MTR_4g121020 48.507 268 132 2 2 267 28 291 9.90e-75 241
MS.gene22816.t1 MTR_4g131580 53.252 246 112 3 5 250 17 259 3.10e-74 238
MS.gene22816.t1 MTR_4g131600 50.607 247 117 2 1 247 16 257 2.97e-72 233
MS.gene22816.t1 MTR_4g131570 50.988 253 114 3 3 250 15 262 3.26e-72 233
MS.gene22816.t1 MTR_3g086100 48.302 265 120 3 4 255 12 272 1.40e-70 228
MS.gene22816.t1 MTR_8g079940 48.315 267 117 4 3 255 23 282 1.40e-69 224
MS.gene22816.t1 MTR_1g013160 77.027 148 34 0 67 214 1 148 1.77e-67 207
MS.gene22816.t1 MTR_3g102590 47.368 266 84 3 2 263 5 218 3.20e-65 204
MS.gene22816.t1 MTR_3g102590 49.167 240 69 3 2 240 5 192 9.18e-60 189
MS.gene22816.t1 MTR_1g032570 48.969 194 82 3 64 247 1 187 8.22e-51 176
MS.gene22816.t1 MTR_4g021760 38.618 246 123 4 5 250 11 228 1.97e-50 169
MS.gene22816.t1 MTR_4g021790 38.956 249 124 5 2 250 7 227 4.61e-49 164
MS.gene22816.t1 MTR_3g102600 64.341 129 46 0 1 129 4 132 1.26e-47 157
MS.gene22816.t1 MTR_4g021855 35.294 272 147 5 2 273 7 249 2.42e-47 160
MS.gene22816.t1 MTR_3g102600 64.341 129 46 0 1 129 4 132 2.72e-47 158
MS.gene22816.t1 MTR_3g086100 46.829 205 92 3 64 255 1 201 2.46e-46 162
MS.gene22816.t1 MTR_4g021845 36.820 239 123 4 2 240 7 217 2.84e-46 155
MS.gene22816.t1 MTR_6g045327 35.510 245 128 5 4 247 13 228 8.00e-39 140
MS.gene22816.t1 MTR_0450s0040 34.025 241 136 3 9 248 8 226 2.54e-38 138
MS.gene22816.t1 MTR_7g026400 35.060 251 136 5 32 278 14 241 4.66e-34 130
MS.gene22816.t1 MTR_4g121020 40.323 186 105 2 84 267 6 187 1.52e-32 126
MS.gene22816.t1 MTR_3g102600 67.470 83 27 0 47 129 1 83 2.65e-32 116
MS.gene22816.t1 MTR_3g102600 67.470 83 27 0 47 129 1 83 5.09e-32 117
MS.gene22816.t1 MTR_5g055260 32.258 248 113 6 2 249 7 199 1.42e-31 118
MS.gene22816.t1 MTR_8g105600 32.727 275 158 8 5 271 9 264 1.18e-29 118
MS.gene22816.t1 MTR_8g032710 30.315 254 157 7 8 243 20 271 1.80e-27 111
MS.gene22816.t1 MTR_6g016850 36.434 129 79 2 1 127 5 132 1.50e-21 89.4
MS.gene22816.t1 MTR_4g023980 31.902 163 84 4 52 214 36 171 4.59e-21 88.6
MS.gene22816.t1 MTR_8g093040 27.935 247 135 5 4 250 13 216 2.91e-20 88.6
MS.gene22816.t1 MTR_8g024260 34.615 130 82 1 6 132 33 162 5.69e-20 90.1
MS.gene22816.t1 MTR_3g092780 27.749 191 115 3 9 177 56 245 1.14e-19 89.4
MS.gene22816.t1 MTR_3g092780 27.749 191 115 3 9 177 56 245 1.29e-19 89.4
MS.gene22816.t1 MTR_3g037390 29.891 184 118 2 6 178 21 204 3.46e-19 87.8
MS.gene22816.t1 MTR_3g092780 27.979 193 113 4 9 177 56 246 6.35e-19 87.0
MS.gene22816.t1 MTR_3g092780 27.979 193 113 4 9 177 56 246 6.84e-19 87.0
MS.gene22816.t1 MTR_1g067110 36.066 122 75 2 9 127 85 206 5.06e-18 84.7
MS.gene22816.t1 MTR_1g067110 36.066 122 75 2 9 127 85 206 5.25e-18 84.3
MS.gene22816.t1 MTR_4g108880 32.031 128 84 1 6 130 30 157 2.41e-17 82.4
MS.gene22816.t1 MTR_4g061360 35.246 122 76 1 9 127 89 210 3.92e-17 82.0
MS.gene22816.t1 MTR_7g118260 33.077 130 78 2 9 132 14 140 1.29e-15 77.4
MS.gene22816.t1 MTR_7g118260 33.600 125 74 2 9 127 14 135 2.89e-15 76.3
MS.gene22816.t1 MTR_4g064730 46.591 88 45 1 162 247 100 187 6.30e-15 73.9
MS.gene22816.t1 MTR_7g115530 64.815 54 18 1 191 243 218 271 1.12e-14 73.2
MS.gene22816.t1 MTR_7g115530 64.815 54 18 1 191 243 218 271 1.67e-14 73.2
MS.gene22816.t1 MTR_7g115530 64.815 54 18 1 191 243 218 271 1.82e-14 73.6
MS.gene22816.t1 MTR_7g115530 64.815 54 18 1 191 243 218 271 2.53e-14 72.8
MS.gene22816.t1 MTR_1g080330 58.621 58 23 1 187 243 171 228 3.82e-14 71.6
MS.gene22816.t1 MTR_1g080330 58.621 58 23 1 187 243 171 228 4.66e-14 72.0
MS.gene22816.t1 MTR_7g089010 53.448 58 27 0 190 247 90 147 2.34e-13 69.3
MS.gene22816.t1 MTR_2g027800 51.562 64 30 1 187 249 35 98 2.60e-13 69.3
MS.gene22816.t1 MTR_2g027800 46.154 78 40 2 187 262 35 112 3.23e-13 69.3
MS.gene22816.t1 MTR_4g106590 32.824 131 75 2 9 126 26 156 4.62e-13 67.4
MS.gene22816.t1 MTR_7g115530 63.462 52 18 1 191 241 218 269 8.26e-13 67.4
MS.gene22816.t1 MTR_7g098250 55.172 58 25 1 187 243 258 315 1.01e-12 68.6
MS.gene22816.t1 MTR_1g053835 51.613 62 29 1 184 244 251 312 1.38e-12 67.8
MS.gene22816.t1 MTR_1g090670 52.542 59 27 1 187 244 40 98 2.24e-12 66.6
MS.gene22816.t1 MTR_5g027440 42.529 87 39 3 191 266 25 111 2.87e-12 65.9
MS.gene22816.t1 MTR_7g115530 62.745 51 18 1 191 240 218 268 4.33e-12 65.9
MS.gene22816.t1 MTR_2g023580 41.489 94 53 2 190 282 54 146 9.49e-12 63.9
MS.gene22816.t1 MTR_7g093030 57.407 54 22 1 191 243 212 265 1.05e-11 64.7
MS.gene22816.t1 MTR_7g093030 50.746 67 30 2 191 254 212 278 1.13e-11 65.1
MS.gene22816.t1 MTR_4g078810 36.145 83 43 1 186 268 153 225 1.26e-11 65.1
MS.gene22816.t1 MTR_6g032990 50.909 55 26 1 191 244 42 96 1.67e-11 64.7
MS.gene22816.t1 MTR_2g090675 36.047 86 45 1 186 271 156 231 1.91e-11 64.3
MS.gene22816.t1 MTR_8g038620 29.286 140 79 3 9 128 9 148 1.94e-11 62.0
MS.gene22816.t1 MTR_8g086410 54.386 57 25 1 190 245 108 164 1.99e-11 64.3
MS.gene22816.t1 MTR_8g086410 54.386 57 25 1 190 245 110 166 1.99e-11 64.3
MS.gene22816.t1 MTR_8g086410 43.210 81 45 1 190 269 110 190 2.76e-11 63.9
MS.gene22816.t1 MTR_7g069660 52.632 57 26 1 190 245 70 126 2.77e-11 63.5
MS.gene22816.t1 MTR_4g081710 47.273 55 28 1 191 244 17 71 2.85e-11 63.5
MS.gene22816.t1 MTR_2g086450 50.909 55 26 1 191 244 45 99 3.17e-11 63.5
MS.gene22816.t1 MTR_4g098870 28.061 196 128 5 4 195 33 219 3.90e-11 63.9
MS.gene22816.t1 MTR_4g098870 50.909 55 27 0 190 244 329 383 4.47e-11 63.5
MS.gene22816.t1 MTR_5g014040 50.909 55 27 0 190 244 310 364 3.92e-11 63.5
MS.gene22816.t1 MTR_5g014040 33.333 126 78 3 4 127 13 134 4.88e-11 63.5
MS.gene22816.t1 MTR_4g098870 28.061 196 128 5 4 195 13 199 4.05e-11 63.5
MS.gene22816.t1 MTR_4g098870 50.909 55 27 0 190 244 309 363 4.73e-11 63.5
MS.gene22816.t1 MTR_4g098870 50.909 55 27 0 190 244 348 402 4.05e-11 63.9
MS.gene22816.t1 MTR_4g098870 28.061 196 128 5 4 195 52 238 5.79e-11 63.2
MS.gene22816.t1 MTR_4g098870 50.909 55 27 0 190 244 329 383 4.14e-11 63.5
MS.gene22816.t1 MTR_4g098870 28.061 196 128 5 4 195 33 219 6.14e-11 63.2
MS.gene22816.t1 MTR_4g098870 50.909 55 27 0 190 244 309 363 4.26e-11 63.5
MS.gene22816.t1 MTR_4g098870 28.061 196 128 5 4 195 13 199 5.41e-11 63.2
MS.gene22816.t1 MTR_5g014040 33.333 126 78 3 4 127 13 134 5.47e-11 63.2
MS.gene22816.t1 MTR_5g041350 47.273 55 28 1 191 244 15 69 7.84e-11 62.4
MS.gene22816.t1 MTR_7g088070 40.909 88 47 3 191 273 50 137 8.18e-11 61.6
MS.gene22816.t1 MTR_1g053800 50.820 61 29 1 187 246 173 233 8.90e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22816.t1 AT4G31920 58.301 259 92 2 3 261 11 253 1.13e-90 280
MS.gene22816.t1 AT2G25180 57.088 261 106 3 3 262 11 266 9.66e-88 273
MS.gene22816.t1 AT3G16857 46.102 295 116 6 5 267 33 316 1.13e-73 238
MS.gene22816.t1 AT3G16857 46.102 295 116 6 5 267 33 316 2.00e-73 238
MS.gene22816.t1 AT5G58080 48.754 281 116 5 3 272 12 275 1.35e-70 229
MS.gene22816.t1 AT2G01760 48.031 254 125 3 1 247 3 256 1.19e-69 221
MS.gene22816.t1 AT1G67710 49.603 252 118 4 7 253 9 256 6.69e-68 220
MS.gene22816.t1 AT2G01760 42.662 293 132 5 1 258 3 294 1.86e-63 205
MS.gene22816.t1 AT3G16857 45.136 257 98 6 43 267 1 246 7.69e-59 198
MS.gene22816.t1 AT5G49240 37.815 238 143 4 5 240 38 272 3.39e-45 155
MS.gene22816.t1 AT2G01760 46.073 191 96 3 64 247 1 191 1.75e-43 151
MS.gene22816.t1 AT1G67710 48.205 195 92 4 64 253 1 191 1.81e-43 154
MS.gene22816.t1 AT3G62670 38.554 249 124 8 24 260 54 285 4.01e-43 152
MS.gene22816.t1 AT1G49190 30.128 312 169 6 4 286 29 320 3.29e-39 142
MS.gene22816.t1 AT5G07210 28.996 269 155 6 9 247 16 278 1.84e-29 117
MS.gene22816.t1 AT1G67710 42.286 175 92 4 84 253 12 182 4.58e-29 115
MS.gene22816.t1 AT2G27070 30.108 279 146 7 7 247 14 281 2.81e-28 114
MS.gene22816.t1 AT5G07210 28.159 277 155 7 9 247 16 286 7.30e-27 110
MS.gene22816.t1 AT1G49190 29.310 232 141 5 4 212 29 260 4.45e-23 99.4
MS.gene22816.t1 AT2G46790 37.398 123 72 2 9 127 37 158 1.42e-21 94.4
MS.gene22816.t1 AT2G46790 37.398 123 72 2 9 127 37 158 1.63e-21 94.4
MS.gene22816.t1 AT5G24470 34.646 127 78 2 6 128 47 172 3.19e-20 90.9
MS.gene22816.t1 AT5G61380 34.375 128 81 1 6 130 16 143 1.24e-19 89.4
MS.gene22816.t1 AT5G60100 37.705 122 73 1 9 127 64 185 1.27e-18 85.5
MS.gene22816.t1 AT5G60100 37.705 122 73 1 9 127 64 185 1.27e-18 85.5
MS.gene22816.t1 AT5G60100 37.705 122 73 1 9 127 64 185 1.27e-18 85.5
MS.gene22816.t1 AT5G60100 37.705 122 73 1 9 127 64 185 1.54e-18 85.9
MS.gene22816.t1 AT5G60100 37.705 122 73 1 9 127 64 185 1.54e-18 85.9
MS.gene22816.t1 AT5G60100 37.705 122 73 1 9 127 64 185 1.54e-18 85.9
MS.gene22816.t1 AT5G60100 37.705 122 73 1 9 127 64 185 2.16e-18 85.5
MS.gene22816.t1 AT5G02810 35.246 122 76 1 9 127 78 199 1.67e-17 83.2
MS.gene22816.t1 AT5G59570 47.436 78 41 0 170 247 120 197 1.65e-16 78.6
MS.gene22816.t1 AT5G59570 47.436 78 41 0 170 247 120 197 1.65e-16 78.6
MS.gene22816.t1 AT3G46640 55.172 58 26 0 190 247 143 200 2.69e-14 72.4
MS.gene22816.t1 AT3G46640 55.172 58 26 0 190 247 143 200 2.69e-14 72.4
MS.gene22816.t1 AT3G46640 55.172 58 26 0 190 247 143 200 3.13e-14 72.4
MS.gene22816.t1 AT2G40970 53.448 58 27 0 190 247 104 161 4.15e-14 70.9
MS.gene22816.t1 AT5G44190 44.304 79 34 1 190 268 146 214 5.32e-14 72.0
MS.gene22816.t1 AT5G44190 44.304 79 34 1 190 268 146 214 5.86e-14 71.6
MS.gene22816.t1 AT3G10760 53.448 58 27 0 190 247 104 161 1.32e-13 70.5
MS.gene22816.t1 AT5G05090 53.448 58 27 0 190 247 80 137 1.60e-13 69.7
MS.gene22816.t1 AT3G13040 53.846 65 29 1 181 244 231 295 9.80e-13 68.6
MS.gene22816.t1 AT3G13040 53.846 65 29 1 181 244 231 295 9.80e-13 68.6
MS.gene22816.t1 AT3G13040 53.846 65 29 1 181 244 231 295 9.80e-13 68.6
MS.gene22816.t1 AT5G45580 49.254 67 33 1 179 244 11 77 3.53e-12 65.9
MS.gene22816.t1 AT4G13640 41.379 87 48 2 187 270 33 119 4.25e-12 65.9
MS.gene22816.t1 AT4G13640 41.379 87 48 2 187 270 33 119 4.83e-12 65.5
MS.gene22816.t1 AT5G45580 54.545 55 24 1 191 244 7 61 5.40e-12 65.1
MS.gene22816.t1 AT1G69580 52.727 55 25 1 191 244 34 88 6.27e-12 65.5
MS.gene22816.t1 AT1G69580 52.727 55 25 1 191 244 34 88 6.30e-12 65.5
MS.gene22816.t1 AT3G24120 40.230 87 49 2 187 270 37 123 1.02e-11 64.7
MS.gene22816.t1 AT3G24120 40.230 87 49 2 187 270 37 123 1.11e-11 64.7
MS.gene22816.t1 AT2G01060 41.000 100 50 4 182 272 4 103 1.27e-11 64.3
MS.gene22816.t1 AT3G04030 50.909 55 26 1 191 244 45 99 1.54e-11 64.7
MS.gene22816.t1 AT3G57040 27.684 177 102 4 9 159 9 185 1.55e-11 63.5
MS.gene22816.t1 AT3G57040 27.684 177 102 4 9 159 9 185 1.55e-11 63.5
MS.gene22816.t1 AT3G04030 50.909 55 26 1 191 244 45 99 1.69e-11 64.7
MS.gene22816.t1 AT3G12730 45.070 71 37 2 187 255 19 89 2.00e-11 63.2
MS.gene22816.t1 AT2G20570 34.426 122 61 3 150 271 121 223 2.11e-11 64.3
MS.gene22816.t1 AT3G04030 48.148 54 28 0 191 244 45 98 3.65e-11 63.5
MS.gene22816.t1 AT5G18240 50.909 55 26 1 191 244 45 99 5.12e-11 63.2
MS.gene22816.t1 AT5G18240 50.909 55 26 1 191 244 45 99 5.12e-11 63.2
MS.gene22816.t1 AT5G18240 50.909 55 26 1 191 244 45 99 5.59e-11 63.2
MS.gene22816.t1 AT5G18240 50.909 55 26 1 191 244 45 99 5.59e-11 63.2
MS.gene22816.t1 AT5G18240 50.909 55 26 1 191 244 45 99 5.62e-11 63.2
MS.gene22816.t1 AT2G20570 40.789 76 35 1 196 271 174 239 7.74e-11 62.8
MS.gene22816.t1 AT5G06800 49.180 61 30 1 185 244 186 246 9.70e-11 61.6

Find 46 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAGAACTTGTCTCAAGATTT+TGG 0.120883 7.4:+1350643 MS.gene22816:CDS
CTTGTCGCGGTTAACAAATT+TGG 0.304801 7.4:+1351821 MS.gene22816:CDS
CCATTTCCACTATCAGAATT+AGG 0.317534 7.4:-1351623 None:intergenic
TGAAAGAGGTGAAGATGATT+TGG 0.332494 7.4:+1351549 MS.gene22816:CDS
ATGAATGATGAGTTTCGAAT+TGG 0.346923 7.4:+1350593 MS.gene22816:CDS
TTCACCTCTTTCAATTGAAC+AGG 0.352913 7.4:-1351539 None:intergenic
TGAGCTTGTTGGACTTGAAA+TGG 0.372169 7.4:+1351341 MS.gene22816:CDS
TTTAAGCTTCTTGAGCTTGT+TGG 0.375182 7.4:+1351330 MS.gene22816:CDS
CAACGAAATCGAGGCTTCTT+CGG 0.398280 7.4:-1351773 None:intergenic
CCTAATTCTGATAGTGGAAA+TGG 0.401332 7.4:+1351623 MS.gene22816:CDS
CCTCCTAGTGTCAATATACA+TGG 0.410772 7.4:+1352528 MS.gene22816:CDS
ATTGCTTTCATAACCAGCTT+AGG 0.420417 7.4:-1351485 None:intergenic
AGTTGAAAAGTAGTAGTTAT+GGG 0.440897 7.4:+1352439 MS.gene22816:CDS
AAACTTGTGGTGTAAGTCAT+TGG 0.441523 7.4:-1351798 None:intergenic
TTCAGTGCTATTCGTGCACT+TGG 0.445352 7.4:-1351237 None:intergenic
CGAGGCTTCTTCGGCGCAGT+TGG 0.453906 7.4:-1351764 None:intergenic
TATGAACATTACTCATTACT+AGG 0.460796 7.4:-1351290 None:intergenic
AGCTTAAATCCATCCATGTC+TGG 0.471845 7.4:-1351315 None:intergenic
AAGTTGAAAAGTAGTAGTTA+TGG 0.482179 7.4:+1352438 MS.gene22816:CDS
TGAAAATGATGATCCTAAGC+TGG 0.496842 7.4:+1351472 MS.gene22816:CDS
ACATGTGGCCAGCCATCTCC+AGG 0.523004 7.4:+1352158 MS.gene22816:CDS
TTGCATAACTTACTTATGAC+AGG 0.526242 7.4:-1351369 None:intergenic
TAAGAATGAAGATGATGTTG+AGG 0.533063 7.4:+1351709 MS.gene22816:CDS
CTCCACGAAGAAAACTTACA+AGG 0.534436 7.4:-1352577 None:intergenic
GATGTGCCTAATTCTGATAG+TGG 0.537306 7.4:+1351617 MS.gene22816:CDS
CGAAGAAGCCTCGATTTCGT+TGG 0.556982 7.4:+1351774 MS.gene22816:CDS
TATTCGTGCACTTGGAGCAG+TGG 0.566879 7.4:-1351229 None:intergenic
GTTCATATGCCAGACATGGA+TGG 0.584384 7.4:+1351306 MS.gene22816:CDS
TAGTGTCAATATACATGGGT+TGG 0.589409 7.4:+1352533 MS.gene22816:CDS
GAAACCTGTTCAATTGAAAG+AGG 0.592307 7.4:+1351535 MS.gene22816:CDS
GTTATGAAAGCAATTGATCT+CGG 0.596446 7.4:+1351494 MS.gene22816:CDS
GAGTATGATCATGAAAATGA+TGG 0.597211 7.4:+1351740 MS.gene22816:CDS
TAGCCACCATATTAGCTCGA+TGG 0.616049 7.4:-1352467 None:intergenic
CGAGCTAATATGGTGGCTAG+TGG 0.620369 7.4:+1352471 MS.gene22816:CDS
AAATCTTACAAAGGAACATG+TGG 0.625593 7.4:+1352143 MS.gene22816:CDS
ATAACTTACTTATGACAGGT+AGG 0.629417 7.4:-1351365 None:intergenic
CTCCTAGTGTCAATATACAT+GGG 0.630450 7.4:+1352529 MS.gene22816:CDS
CCATGTATATTGACACTAGG+AGG 0.632916 7.4:-1352528 None:intergenic
GAATGTTGAAAATCTTACAA+AGG 0.637383 7.4:+1352134 MS.gene22816:CDS
ACACCACAAGTTTCTTGTCG+CGG 0.645407 7.4:+1351808 MS.gene22816:CDS
CTCGAAAGGTGTCAATACCA+TGG 0.650196 7.4:+1350674 MS.gene22816:CDS
TCATTGGACCAACGAAATCG+AGG 0.660616 7.4:-1351782 None:intergenic
TAATGTTCATATGCCAGACA+TGG 0.666265 7.4:+1351302 MS.gene22816:CDS
TAACCGCGACAAGAAACTTG+TGG 0.669528 7.4:-1351811 None:intergenic
AACCCATGTATATTGACACT+AGG 0.674841 7.4:-1352531 None:intergenic
AAACCATCGAGCTAATATGG+TGG 0.705104 7.4:+1352464 MS.gene22816:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATTAATATCATTATGAA+AGG - chr7.4:1351185-1351204 None:intergenic 10.0%
!! ATAATATTAATATCAACATT+GGG - chr7.4:1351967-1351986 None:intergenic 10.0%
!! CATAATATTAATATCAACAT+TGG - chr7.4:1351968-1351987 None:intergenic 15.0%
!!! TTTACAAACACTTTAATTTA+GGG + chr7.4:1352370-1352389 MS.gene22816:intron 15.0%
!!! TTTTACAAACACTTTAATTT+AGG + chr7.4:1352369-1352388 MS.gene22816:intron 15.0%
!! ATCATTAAATTGTAATCGTA+AGG + chr7.4:1350760-1350779 MS.gene22816:intron 20.0%
!! TCATTAAATTGTAATCGTAA+GGG + chr7.4:1350761-1350780 MS.gene22816:intron 20.0%
!! TTAATTTAGGGAAATTTACT+TGG + chr7.4:1352382-1352401 MS.gene22816:intron 20.0%
!!! TAATATAATTGATTCGTACA+AGG - chr7.4:1350940-1350959 None:intergenic 20.0%
!!! TGTGAACTTATGTTATTTTT+TGG + chr7.4:1351088-1351107 MS.gene22816:intron 20.0%
! AAGAGATAAAGAAAAATACC+TGG - chr7.4:1352179-1352198 None:intergenic 25.0%
! AAGTTGAAAAGTAGTAGTTA+TGG + chr7.4:1352438-1352457 MS.gene22816:CDS 25.0%
! AATTAGAACGATAACGTTAA+CGG + chr7.4:1350873-1350892 MS.gene22816:intron 25.0%
! AGCAATAGATAAAAGATTTG+AGG - chr7.4:1351896-1351915 None:intergenic 25.0%
! AGTTGAAAAGTAGTAGTTAT+GGG + chr7.4:1352439-1352458 MS.gene22816:CDS 25.0%
! ATGTATCAGTAACAAAAGAT+TGG - chr7.4:1351399-1351418 None:intergenic 25.0%
! GAATGTTGAAAATCTTACAA+AGG + chr7.4:1352134-1352153 MS.gene22816:CDS 25.0%
! GTGTTTGTAAAATGTAAACT+AGG - chr7.4:1352361-1352380 None:intergenic 25.0%
! TATGAACATTACTCATTACT+AGG - chr7.4:1351293-1351312 None:intergenic 25.0%
! TTGTAAAATGTAAACTAGGT+AGG - chr7.4:1352357-1352376 None:intergenic 25.0%
! TTTATCAAAGATGTTTGAGT+AGG + chr7.4:1351931-1351950 MS.gene22816:intron 25.0%
!! AATCTTTGAGAGAATTTGAT+CGG - chr7.4:1351017-1351036 None:intergenic 25.0%
!! ATCAAGTTAAGAATCTTTTG+TGG - chr7.4:1352114-1352133 None:intergenic 25.0%
!! TTATCCTTTCTTTTTCTAGA+TGG - chr7.4:1351692-1351711 None:intergenic 25.0%
AAAGCCATCTAGAAAAAGAA+AGG + chr7.4:1351685-1351704 MS.gene22816:CDS 30.0%
AAATCTTACAAAGGAACATG+TGG + chr7.4:1352143-1352162 MS.gene22816:CDS 30.0%
ATAACTTACTTATGACAGGT+AGG - chr7.4:1351368-1351387 None:intergenic 30.0%
ATCGAATGAATCCAACAATT+TGG - chr7.4:1351135-1351154 None:intergenic 30.0%
ATGAATGATGAGTTTCGAAT+TGG + chr7.4:1350593-1350612 MS.gene22816:CDS 30.0%
GAGTATGATCATGAAAATGA+TGG + chr7.4:1351740-1351759 MS.gene22816:CDS 30.0%
GATCAAATTCTCTCAAAGAT+TGG + chr7.4:1351016-1351035 MS.gene22816:intron 30.0%
GTTATGAAAGCAATTGATCT+CGG + chr7.4:1351494-1351513 MS.gene22816:CDS 30.0%
TAAAGAAAAATACCTGGAGA+TGG - chr7.4:1352173-1352192 None:intergenic 30.0%
TAAATTGTAATCGTAAGGGA+CGG + chr7.4:1350765-1350784 MS.gene22816:intron 30.0%
TAAGAATGAAGATGATGTTG+AGG + chr7.4:1351709-1351728 MS.gene22816:CDS 30.0%
TTGCATAACTTACTTATGAC+AGG - chr7.4:1351372-1351391 None:intergenic 30.0%
TTTAACTGCATCCAAATTGT+TGG + chr7.4:1351121-1351140 MS.gene22816:intron 30.0%
! AAAGAAAAAGAAGCATACCA+TGG - chr7.4:1350694-1350713 None:intergenic 30.0%
! CACTTCTAATCTAAGGTTAT+CGG - chr7.4:1350796-1350815 None:intergenic 30.0%
! CATTTTCTTCGATACTGTTT+AGG + chr7.4:1350971-1350990 MS.gene22816:intron 30.0%
!!! AGTTGTTATTTTGTTTGCAG+AGG + chr7.4:1352083-1352102 MS.gene22816:intron 30.0%
AAACTTGTGGTGTAAGTCAT+TGG - chr7.4:1351801-1351820 None:intergenic 35.0%
AACCCATGTATATTGACACT+AGG - chr7.4:1352534-1352553 None:intergenic 35.0%
AGACAATCATGACAAAGACA+CGG + chr7.4:1352223-1352242 MS.gene22816:intron 35.0%
ATTACAATGCCTTAGCGAAA+AGG - chr7.4:1350856-1350875 None:intergenic 35.0%
ATTGCTTTCATAACCAGCTT+AGG - chr7.4:1351488-1351507 None:intergenic 35.0%
CCATTTCCACTATCAGAATT+AGG - chr7.4:1351626-1351645 None:intergenic 35.0%
CTCCTAGTGTCAATATACAT+GGG + chr7.4:1352529-1352548 MS.gene22816:CDS 35.0%
GAAAAACCATCGAGCTAATA+TGG + chr7.4:1352461-1352480 MS.gene22816:CDS 35.0%
GAAACCTGTTCAATTGAAAG+AGG + chr7.4:1351535-1351554 MS.gene22816:CDS 35.0%
TAATGTTCATATGCCAGACA+TGG + chr7.4:1351302-1351321 MS.gene22816:CDS 35.0%
TAGTGTCAATATACATGGGT+TGG + chr7.4:1352533-1352552 MS.gene22816:CDS 35.0%
TGAAAATGATGATCCTAAGC+TGG + chr7.4:1351472-1351491 MS.gene22816:CDS 35.0%
TGAAAGAGGTGAAGATGATT+TGG + chr7.4:1351549-1351568 MS.gene22816:CDS 35.0%
TTCACCTCTTTCAATTGAAC+AGG - chr7.4:1351542-1351561 None:intergenic 35.0%
TTTAAGCTTCTTGAGCTTGT+TGG + chr7.4:1351330-1351349 MS.gene22816:CDS 35.0%
! CAGAACTTGTCTCAAGATTT+TGG + chr7.4:1350643-1350662 MS.gene22816:CDS 35.0%
! TCTTTAGATCCTTTTCGCTA+AGG + chr7.4:1350844-1350863 MS.gene22816:intron 35.0%
! TTTTGCACATTGAAAGCAGA+GGG - chr7.4:1351865-1351884 None:intergenic 35.0%
! TTTTTGCACATTGAAAGCAG+AGG - chr7.4:1351866-1351885 None:intergenic 35.0%
!! CCTAATTCTGATAGTGGAAA+TGG + chr7.4:1351623-1351642 MS.gene22816:CDS 35.0%
!! TTATTTTGTTTGCAGAGGCT+AGG + chr7.4:1352088-1352107 MS.gene22816:intron 35.0%
AAACCATCGAGCTAATATGG+TGG + chr7.4:1352464-1352483 MS.gene22816:CDS 40.0%
AGCTTAAATCCATCCATGTC+TGG - chr7.4:1351318-1351337 None:intergenic 40.0%
CCATGTATATTGACACTAGG+AGG - chr7.4:1352531-1352550 None:intergenic 40.0%
CCTCCTAGTGTCAATATACA+TGG + chr7.4:1352528-1352547 MS.gene22816:CDS 40.0%
CTTGTCGCGGTTAACAAATT+TGG + chr7.4:1351821-1351840 MS.gene22816:CDS 40.0%
GATGTGCCTAATTCTGATAG+TGG + chr7.4:1351617-1351636 MS.gene22816:CDS 40.0%
TTGTTGGATTCATTCGATGC+AGG + chr7.4:1351137-1351156 MS.gene22816:intron 40.0%
!! CGTCATAGCAAGAGTATTTG+AGG - chr7.4:1352032-1352051 None:intergenic 40.0%
!! TGAGCTTGTTGGACTTGAAA+TGG + chr7.4:1351341-1351360 MS.gene22816:CDS 40.0%
!! TTTTGGAGAGACTTCTCGAA+AGG + chr7.4:1350660-1350679 MS.gene22816:CDS 40.0%
ACACCACAAGTTTCTTGTCG+CGG + chr7.4:1351808-1351827 MS.gene22816:CDS 45.0%
CAACGAAATCGAGGCTTCTT+CGG - chr7.4:1351776-1351795 None:intergenic 45.0%
GAAAAATACCTGGAGATGGC+TGG - chr7.4:1352169-1352188 None:intergenic 45.0%
GCAGCGACACTTCTAATCTA+AGG - chr7.4:1350803-1350822 None:intergenic 45.0%
GTTCATATGCCAGACATGGA+TGG + chr7.4:1351306-1351325 MS.gene22816:CDS 45.0%
TAACCGCGACAAGAAACTTG+TGG - chr7.4:1351814-1351833 None:intergenic 45.0%
TAGCCACCATATTAGCTCGA+TGG - chr7.4:1352470-1352489 None:intergenic 45.0%
TCATTGGACCAACGAAATCG+AGG - chr7.4:1351785-1351804 None:intergenic 45.0%
! TTCAGTGCTATTCGTGCACT+TGG - chr7.4:1351240-1351259 None:intergenic 45.0%
!! CTCGAAAGGTGTCAATACCA+TGG + chr7.4:1350674-1350693 MS.gene22816:CDS 45.0%
CGAAGAAGCCTCGATTTCGT+TGG + chr7.4:1351774-1351793 MS.gene22816:CDS 50.0%
CGAGCTAATATGGTGGCTAG+TGG + chr7.4:1352471-1352490 MS.gene22816:CDS 50.0%
TATTCGTGCACTTGGAGCAG+TGG - chr7.4:1351232-1351251 None:intergenic 50.0%
ACATGTGGCCAGCCATCTCC+AGG + chr7.4:1352158-1352177 MS.gene22816:CDS 60.0%
CGAGGCTTCTTCGGCGCAGT+TGG - chr7.4:1351767-1351786 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 1350593 1352584 1350593 ID=MS.gene22816
chr7.4 mRNA 1350593 1352584 1350593 ID=MS.gene22816.t1;Parent=MS.gene22816
chr7.4 exon 1350593 1350695 1350593 ID=MS.gene22816.t1.exon1;Parent=MS.gene22816.t1
chr7.4 CDS 1350593 1350695 1350593 ID=cds.MS.gene22816.t1;Parent=MS.gene22816.t1
chr7.4 exon 1351226 1351378 1351226 ID=MS.gene22816.t1.exon2;Parent=MS.gene22816.t1
chr7.4 CDS 1351226 1351378 1351226 ID=cds.MS.gene22816.t1;Parent=MS.gene22816.t1
chr7.4 exon 1351465 1351851 1351465 ID=MS.gene22816.t1.exon3;Parent=MS.gene22816.t1
chr7.4 CDS 1351465 1351851 1351465 ID=cds.MS.gene22816.t1;Parent=MS.gene22816.t1
chr7.4 exon 1352103 1352179 1352103 ID=MS.gene22816.t1.exon4;Parent=MS.gene22816.t1
chr7.4 CDS 1352103 1352179 1352103 ID=cds.MS.gene22816.t1;Parent=MS.gene22816.t1
chr7.4 exon 1352426 1352584 1352426 ID=MS.gene22816.t1.exon5;Parent=MS.gene22816.t1
chr7.4 CDS 1352426 1352584 1352426 ID=cds.MS.gene22816.t1;Parent=MS.gene22816.t1
Gene Sequence

>MS.gene22816

ATGAATGATGAGTTTCGAATTGGAATGAATATTCTTGCTGTTGATGATGACAGAACTTGTCTCAAGATTTTGGAGAGACTTCTCGAAAGGTGTCAATACCATGGTATGCTTCTTTTTCTTTTTTAAATATTAAAAAATTGTTTTTGACTATGAAGCAGTGACACGACATCATTAAATTGTAATCGTAAGGGACGGTGCGTCCGATAACCTTAGATTAGAAGTGTCGCTGCTACAAAAGTTTTAGATTTGATTCTTTAGATCCTTTTCGCTAAGGCATTGTAATTAGAACGATAACGTTAACGGTTTCTTTTTCTTCTATTTTTTCTACATTAGTCTAGCTAATGCCTTGTACGAATCAATTATATTAGTTTAGTCTTACATTTTCTTCGATACTGTTTAGGTTCTACTAAATTATATTTGACCGATCAAATTCTCTCAAAGATTGGACGTTTTTAAAGTTCACAGTTTTAAAAAAAAATCTTGTGTCTAATCTTTTGTGAACTTATGTTATTTTTTGGATTTCAAATATTTAACTGCATCCAAATTGTTGGATTCATTCGATGCAGGACATAATGTATCAATTATTTTTCCTTTCATAATGATATTAATTTTAGTTATAATTATTTGTTGCAGTAACCACTGCTCCAAGTGCACGAATAGCACTGAATTTGTTAAGAGAAAACAAAAACAATTTTGACCTAGTAATGAGTAATGTTCATATGCCAGACATGGATGGATTTAAGCTTCTTGAGCTTGTTGGACTTGAAATGGACCTACCTGTCATAAGTAAGTTATGCAATTCTCCAATCTTTTGTTACTGATACATTTATAACAAATTATCAAGTGTTTTAATTACAAAGTTTTGTTTTCAGTGTTTTCTGAAAATGATGATCCTAAGCTGGTTATGAAAGCAATTGATCTCGGTGCTTGTGATTATCTGCTGAAACCTGTTCAATTGAAAGAGGTGAAGATGATTTGGCAACATGTAATCAGAAAGAAGAAAATTAGCAAAAGAAGCAATCACGATGTGCCTAATTCTGATAGTGGAAATGGAATATATTCAACTGTGACAAAAAATTCTGATAAAATTGAAAAGCCATCTAGAAAAAGAAAGGATAAGAATGAAGATGATGTTGAGGAAGAGAATGAGTATGATCATGAAAATGATGGTCCAACTGCGCCGAAGAAGCCTCGATTTCGTTGGTCCAATGACTTACACCACAAGTTTCTTGTCGCGGTTAACAAATTTGGCGTTGAAAGTATGTTCTTCCCTCTGCTTTCAATGTGCAAAAACAACTATCCTCAAATCTTTTATCTATTGCTATATTCGTCTTTATTTTTATCAAAGATGTTTGAGTAGGTTATAGCAATCCCAATGTTGATATTAATATTATGATATGAAAACGAAAATTATACTTTGTTTTATTTGAAGATTTCCTCAAATACTCTTGCTATGACGTTTTTCAACATTGAACTGTGTTTCTCATTATAGTTGTTATTTTGTTTGCAGAGGCTAGGCCACAAAAGATTCTTAACTTGATGAATGTTGAAAATCTTACAAAGGAACATGTGGCCAGCCATCTCCAGGTATTTTTCTTTATCTCTTATGCTTTAGCTTTTTAAGCTATGAAGACAATCATGACAAAGACACGGATGCAAGACATGACACTGACATAATGACACTGCTATAAATTTATAAATTTTTAGTAATTAAATTAAATGTTAAATTAATTTCATGTATAGTGTCAAACACTAGACACACCTACCTAGTTTACATTTTACAAACACTTTAATTTAGGGAAATTTACTTGGTTTTTGTTTTTAAATCTGCAGAAATATAGACTAAAGTTGAAAAGTAGTAGTTATGGGAAAAACCATCGAGCTAATATGGTGGCTAGTGGTTCTGATACATCATCTCACTCAAGAATTGACACTCCTCCTAGTGTCAATATACATGGGTTGGCTACTCTTGATTTAAACAACTCCTTGTAA
Protein sequence

>MS.gene22816.t1

MNDEFRIGMNILAVDDDRTCLKILERLLERCQYHVTTAPSARIALNLLRENKNNFDLVMSNVHMPDMDGFKLLELVGLEMDLPVIMFSENDDPKLVMKAIDLGACDYLLKPVQLKEVKMIWQHVIRKKKISKRSNHDVPNSDSGNGIYSTVTKNSDKIEKPSRKRKDKNEDDVEEENEYDHENDGPTAPKKPRFRWSNDLHHKFLVAVNKFGVEKARPQKILNLMNVENLTKEHVASHLQKYRLKLKSSSYGKNHRANMVASGSDTSSHSRIDTPPSVNIHGLATLDLNNSL