AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene22853

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22853.t1 MTR_8g095330 49.669 151 57 3 77 222 42 178 1.23e-32 122
MS.gene22853.t1 MTR_3g097320 94.915 59 3 0 77 135 41 99 5.55e-32 120
MS.gene22853.t1 MTR_1g034030 89.831 59 6 0 77 135 61 119 7.10e-31 117
MS.gene22853.t1 MTR_1g034030 89.831 59 6 0 77 135 61 119 1.01e-30 117
MS.gene22853.t1 MTR_4g086855 89.831 59 6 0 77 135 60 118 1.15e-30 117
MS.gene22853.t1 MTR_2g006330 91.525 59 5 0 77 135 41 99 1.28e-30 116
MS.gene22853.t1 MTR_5g064540 84.746 59 9 0 77 135 41 99 6.59e-30 112
MS.gene22853.t1 MTR_4g099240 86.441 59 8 0 77 135 42 100 2.78e-29 112
MS.gene22853.t1 MTR_5g064540 84.746 59 9 0 77 135 41 99 5.02e-29 112
MS.gene22853.t1 MTR_5g018050 44.371 151 65 4 77 222 55 191 5.18e-29 110
MS.gene22853.t1 MTR_5g018050 44.371 151 65 4 77 222 22 158 8.98e-29 111
MS.gene22853.t1 MTR_8g089975 86.441 59 8 0 77 135 41 99 1.10e-28 111
MS.gene22853.t1 MTR_5g018050 44.371 151 65 4 77 222 55 191 1.95e-28 110
MS.gene22853.t1 MTR_8g079560 86.441 59 8 0 77 135 60 118 2.91e-28 110
MS.gene22853.t1 MTR_3g086940 83.051 59 10 0 77 135 42 100 5.05e-27 107
MS.gene22853.t1 MTR_5g013550 43.046 151 67 3 77 222 43 179 2.36e-26 105
MS.gene22853.t1 MTR_8g088740 71.186 59 17 0 77 135 41 99 4.09e-25 98.6
MS.gene22853.t1 MTR_4g099220 40.132 152 72 4 77 223 48 185 5.28e-25 101
MS.gene22853.t1 MTR_7g116540 77.419 62 13 1 160 221 38 98 2.92e-22 88.2
MS.gene22853.t1 MTR_6g044740 55.422 83 32 2 154 232 433 514 9.93e-18 82.4
MS.gene22853.t1 MTR_6g083020 53.012 83 34 2 154 232 436 517 2.56e-16 78.6
MS.gene22853.t1 MTR_6g044830 51.807 83 35 2 154 232 412 493 1.02e-15 76.6
MS.gene22853.t1 MTR_1g110110 37.195 164 75 8 90 243 369 514 1.16e-15 76.6
MS.gene22853.t1 MTR_1g110120 51.807 83 35 2 154 232 429 510 3.44e-15 75.1
MS.gene22853.t1 MTR_8g078940 31.841 201 87 7 64 235 339 518 6.50e-15 74.3
MS.gene22853.t1 MTR_7g082530 49.398 83 37 2 154 232 462 543 1.35e-14 73.2
MS.gene22853.t1 MTR_7g082530 49.398 83 37 2 154 232 462 543 1.39e-14 73.2
MS.gene22853.t1 MTR_7g116530 51.282 78 26 2 77 153 29 95 2.10e-14 67.4
MS.gene22853.t1 MTR_1g110180 46.739 92 42 3 145 232 432 520 3.75e-14 72.0
MS.gene22853.t1 MTR_6g082700 32.121 165 83 6 77 232 157 301 1.93e-13 69.7
MS.gene22853.t1 MTR_1g110130 35.849 159 82 5 79 232 328 471 1.99e-13 69.7
MS.gene22853.t1 MTR_5g055310 48.810 84 38 2 153 232 413 495 4.11e-13 68.9
MS.gene22853.t1 MTR_3g111510 32.402 179 89 6 67 235 363 519 2.94e-12 66.6
MS.gene22853.t1 MTR_5g075100 55.932 59 26 0 76 134 63 121 3.42e-12 65.9
MS.gene22853.t1 MTR_5g018570 30.244 205 92 7 61 235 329 512 3.60e-12 66.2
MS.gene22853.t1 MTR_1g076370 33.784 148 67 6 77 215 62 187 1.34e-11 64.3
MS.gene22853.t1 MTR_6g012990 33.813 139 76 4 77 215 60 182 2.53e-11 63.5
MS.gene22853.t1 MTR_6g012990 33.813 139 76 4 77 215 60 182 2.56e-11 63.2
MS.gene22853.t1 MTR_6g048250 34.532 139 75 5 77 215 60 182 2.98e-11 62.8
MS.gene22853.t1 MTR_6g048250 34.532 139 75 5 77 215 60 182 3.32e-11 63.2
MS.gene22853.t1 MTR_1g013700 32.432 148 72 6 76 216 53 179 3.40e-11 63.2
MS.gene22853.t1 MTR_6g048250 34.532 139 75 5 77 215 60 182 4.00e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene22853.t1 AT1G10940 49.007 151 66 3 77 222 41 185 1.46e-31 119
MS.gene22853.t1 AT1G10940 49.007 151 58 4 77 222 41 177 1.80e-31 119
MS.gene22853.t1 AT1G60940 47.059 153 58 4 77 222 41 177 2.61e-31 119
MS.gene22853.t1 AT1G60940 47.059 153 58 4 77 222 41 177 2.61e-31 119
MS.gene22853.t1 AT5G63650 48.344 151 59 4 77 222 41 177 3.85e-31 118
MS.gene22853.t1 AT4G33950 89.831 59 6 0 77 135 58 116 7.86e-31 117
MS.gene22853.t1 AT4G33950 89.831 59 6 0 77 135 58 116 1.13e-30 117
MS.gene22853.t1 AT5G66880 68.293 82 24 1 77 158 59 138 2.52e-30 116
MS.gene22853.t1 AT5G66880 68.293 82 24 1 77 158 59 138 2.52e-30 116
MS.gene22853.t1 AT3G50500 88.136 59 7 0 77 135 60 118 2.70e-30 116
MS.gene22853.t1 AT3G50500 88.136 59 7 0 77 135 60 118 3.55e-30 115
MS.gene22853.t1 AT4G40010 45.695 151 63 4 77 222 41 177 3.49e-29 113
MS.gene22853.t1 AT5G08590 86.441 59 8 0 77 135 41 99 4.01e-29 112
MS.gene22853.t1 AT4G11373 85.000 60 9 0 77 136 10 69 3.50e-28 104
MS.gene22853.t1 AT2G23030 46.358 151 62 3 77 222 41 177 5.50e-28 109
MS.gene22853.t1 AT1G78290 81.356 59 11 0 77 135 41 99 4.01e-25 102
MS.gene22853.t1 AT1G78290 81.356 59 11 0 77 135 41 99 4.01e-25 102
MS.gene22853.t1 AT5G38210 37.736 159 79 5 79 232 409 552 1.11e-17 82.4
MS.gene22853.t1 AT5G38210 37.736 159 79 5 79 232 391 534 1.55e-17 82.0
MS.gene22853.t1 AT5G38210 37.736 159 79 5 79 232 397 540 1.68e-17 82.0
MS.gene22853.t1 AT1G66880 37.107 159 80 5 79 232 385 528 5.63e-16 77.4
MS.gene22853.t1 AT1G66880 37.107 159 80 5 79 232 627 770 6.13e-16 77.4
MS.gene22853.t1 AT1G66880 37.107 159 80 5 79 232 1006 1149 7.18e-16 77.4
MS.gene22853.t1 AT2G23450 32.927 164 83 4 79 235 387 530 1.48e-15 76.3
MS.gene22853.t1 AT2G23450 32.927 164 83 4 79 235 387 530 1.48e-15 76.3
MS.gene22853.t1 AT1G18390 36.054 147 74 5 91 232 389 520 6.77e-15 74.3
MS.gene22853.t1 AT1G18390 36.054 147 74 5 91 232 395 526 8.12e-15 73.9
MS.gene22853.t1 AT1G25390 46.237 93 43 3 144 232 385 474 1.80e-13 70.1
MS.gene22853.t1 AT1G25390 46.237 93 43 3 144 232 385 474 1.80e-13 70.1
MS.gene22853.t1 AT2G25090 31.544 149 80 3 76 216 56 190 7.96e-13 67.8
MS.gene22853.t1 AT2G29000 33.784 148 74 4 93 232 618 749 5.43e-12 65.9
MS.gene22853.t1 AT3G01090 33.094 139 77 4 77 215 83 205 7.28e-12 65.1
MS.gene22853.t1 AT3G01090 33.094 139 77 4 77 215 60 182 7.98e-12 65.1
MS.gene22853.t1 AT3G01090 33.094 139 77 4 77 215 60 182 7.98e-12 65.1
MS.gene22853.t1 AT5G35410 32.394 142 73 4 79 216 54 176 2.22e-11 63.5
MS.gene22853.t1 AT5G35410 32.394 142 73 4 79 216 54 176 2.49e-11 63.5
MS.gene22853.t1 AT5G16900 34.559 136 59 5 108 232 455 571 2.52e-11 63.5
MS.gene22853.t1 AT5G16900 34.559 136 59 5 108 232 641 757 2.92e-11 63.5
MS.gene22853.t1 AT5G16900 34.559 136 59 5 108 232 641 757 2.92e-11 63.5
MS.gene22853.t1 AT5G48740 32.667 150 78 5 91 232 657 791 3.00e-11 63.5
MS.gene22853.t1 AT1G16120 30.539 167 89 5 77 232 462 612 4.62e-11 62.8

Find 51 sgRNAs with CRISPR-Local

Find 137 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTACAAGGAGCAGGGGCTTT+TGG 0.237530 7.4:-1685824 MS.gene22853:CDS
TTTCAGGAAAGCGTAGATTT+TGG 0.265085 7.4:-1684967 MS.gene22853:CDS
CTATGACTTTCTTGTATAGA+TGG 0.270578 7.4:+1686297 None:intergenic
CCATATCACAACGACATAAA+TGG 0.296698 7.4:+1687069 None:intergenic
TCCTTGAAGCGAATTATATT+CGG 0.307244 7.4:+1686068 None:intergenic
CTAATAGAGTGGAGGCAATT+AGG 0.312378 7.4:-1684991 MS.gene22853:CDS
CTTGTGCACCTGTTCGAATA+TGG 0.353234 7.4:+1685083 None:intergenic
CCATTTATGTCGTTGTGATA+TGG 0.357277 7.4:-1687069 MS.gene22853:CDS
TCAAGGATGAAACTCATTCT+TGG 0.368026 7.4:-1686479 MS.gene22853:intron
TCAGAATGATCTAGATAAAC+TGG 0.376896 7.4:+1685055 None:intergenic
ATACAAGAAAGTCATAGAAT+TGG 0.400740 7.4:-1686291 MS.gene22853:CDS
GACTTTCTTGTATAGATGGT+TGG 0.419580 7.4:+1686301 None:intergenic
CCCGAATATAATTCGCTTCA+AGG 0.424576 7.4:-1686069 MS.gene22853:CDS
CGAAGATGAGGTTACTTCAA+TGG 0.436810 7.4:-1685848 MS.gene22853:CDS
CTGCGAGAGCATGGAATTCA+AGG 0.437944 7.4:-1686496 MS.gene22853:CDS
CTGCAGAACTACTAATGTAT+TGG 0.453259 7.4:+1686334 None:intergenic
TTCAATGGGCTACAAGGAGC+AGG 0.462180 7.4:-1685833 MS.gene22853:CDS
ATTACTATTGCTAAATGAGT+AGG 0.476187 7.4:+1685928 None:intergenic
TGTCGTTGTGATATGGATTG+TGG 0.477493 7.4:-1687062 MS.gene22853:CDS
TTACTATTGCTAAATGAGTA+GGG 0.482655 7.4:+1685929 None:intergenic
CCTTGAAGCGAATTATATTC+GGG 0.486169 7.4:+1686069 None:intergenic
GAAGATGAGGTTACTTCAAT+GGG 0.488110 7.4:-1685847 MS.gene22853:CDS
CGCGGATGCACTTGCAGTTG+TGG 0.489116 7.4:-1687092 MS.gene22853:CDS
GATCAAAGCCATATTCGAAC+AGG 0.496465 7.4:-1685091 MS.gene22853:CDS
TTATCAACCACAGGTCTCTT+CGG 0.501774 7.4:-1686094 MS.gene22853:CDS
AAGTGATAGTTCTAATAGAG+TGG 0.507803 7.4:-1685002 MS.gene22853:CDS
AACTAACAGCTGCGAGAGCA+TGG 0.515868 7.4:-1686505 MS.gene22853:intron
TTTGAGATAATCTGTAATGT+TGG 0.524509 7.4:-1685880 MS.gene22853:CDS
AGATAATCTGTAATGTTGGT+CGG 0.529867 7.4:-1685876 MS.gene22853:CDS
ATTGGAACAAGAGATAACTT+TGG 0.536287 7.4:-1686273 MS.gene22853:CDS
ATAGTAATGGAGTATGCAGC+TGG 0.543157 7.4:-1685913 MS.gene22853:CDS
GGTTACTTCAATGGGCTACA+AGG 0.545651 7.4:-1685839 MS.gene22853:CDS
GGAACAAGAGATAACTTTGG+TGG 0.553907 7.4:-1686270 MS.gene22853:CDS
CATATTCGAACAGGTGCACA+AGG 0.558852 7.4:-1685082 MS.gene22853:CDS
TACTCATTTAGCAATAGTAA+TGG 0.559152 7.4:-1685926 MS.gene22853:CDS
TTTCCAGATTGATGAGAATG+TGG 0.567042 7.4:-1686126 MS.gene22853:intron
TCAATGGGCTACAAGGAGCA+GGG 0.573146 7.4:-1685832 MS.gene22853:CDS
TAATGGAGTATGCAGCTGGA+GGG 0.587159 7.4:-1685909 MS.gene22853:CDS
AATGGAGTATGCAGCTGGAG+GGG 0.589893 7.4:-1685908 MS.gene22853:CDS
CTAGAGAGATTATCAACCAC+AGG 0.592807 7.4:-1686103 MS.gene22853:CDS
TGGTCGGTTCAGCGAAGATG+AGG 0.609385 7.4:-1685860 MS.gene22853:CDS
GCCGACTTTCATGCAAAAGT+AGG 0.613734 7.4:-1685145 MS.gene22853:CDS
GTAATGGAGTATGCAGCTGG+AGG 0.638186 7.4:-1685910 MS.gene22853:CDS
TACTATTGCTAAATGAGTAG+GGG 0.638850 7.4:+1685930 None:intergenic
GGCTAGGATGAATATTGCAG+TGG 0.646337 7.4:-1685236 MS.gene22853:CDS
TGATAGTTCTAATAGAGTGG+AGG 0.652354 7.4:-1684999 MS.gene22853:CDS
CAATGGGCTACAAGGAGCAG+GGG 0.665049 7.4:-1685831 MS.gene22853:CDS
TCGGGTGCCGAAGAGACCTG+TGG 0.684900 7.4:+1686087 None:intergenic
GAATATAATTCGCTTCAAGG+AGG 0.726990 7.4:-1686066 MS.gene22853:intron
GATATGGATTGTGGATGCTG+CGG 0.730240 7.4:-1687053 MS.gene22853:CDS
ATATTCGAACAGGTGCACAA+GGG 0.753564 7.4:-1685081 MS.gene22853:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGTTTAAATAATTATTAAG+GGG + chr7.4:1685193-1685212 None:intergenic 10.0%
!!! ATATTTTTTAAAAAGTGAAA+TGG + chr7.4:1685832-1685851 None:intergenic 10.0%
!! TACATGAAAAATATCAAAAT+TGG + chr7.4:1685625-1685644 None:intergenic 15.0%
!!! AATTGTTTGATCTTTTTATA+TGG + chr7.4:1686359-1686378 None:intergenic 15.0%
!!! TAAGATTATGAATTTTCAAT+TGG - chr7.4:1686378-1686397 MS.gene22853:intron 15.0%
!! ATAAACAACCAAAAAATATG+TGG + chr7.4:1685494-1685513 None:intergenic 20.0%
!! TGGAATCAATAAAATTGTTA+AGG + chr7.4:1685370-1685389 None:intergenic 20.0%
!!! AAAACTATTATTTTGACATC+TGG - chr7.4:1685880-1685899 MS.gene22853:CDS 20.0%
!!! AAACTATTATTTTGACATCT+GGG - chr7.4:1685881-1685900 MS.gene22853:CDS 20.0%
!!! TTAGTTTACCACATATTTTT+TGG - chr7.4:1685483-1685502 MS.gene22853:intron 20.0%
! AAAACATACTCAATTTCCTA+TGG + chr7.4:1686484-1686503 None:intergenic 25.0%
! AAATAATTATTAAGGGGAAG+AGG + chr7.4:1685187-1685206 None:intergenic 25.0%
! ATACAAGAAAGTCATAGAAT+TGG - chr7.4:1685779-1685798 MS.gene22853:intron 25.0%
! ATTACTATTGCTAAATGAGT+AGG + chr7.4:1686145-1686164 None:intergenic 25.0%
! ATTGGCGTCTATATTTATAT+AGG - chr7.4:1685711-1685730 MS.gene22853:intron 25.0%
! CAAATATTATGTCGTTACAT+GGG - chr7.4:1686698-1686717 MS.gene22853:intron 25.0%
! CAATATAGGGATAATTAACT+TGG - chr7.4:1686809-1686828 MS.gene22853:intron 25.0%
! TAACGACATAATATTTGGAT+GGG + chr7.4:1686695-1686714 None:intergenic 25.0%
! TACTCATTTAGCAATAGTAA+TGG - chr7.4:1686144-1686163 MS.gene22853:intron 25.0%
! TATAATGACTATTGACAGTA+AGG + chr7.4:1686322-1686341 None:intergenic 25.0%
! TCATATGATATTTCTTGTCT+AGG - chr7.4:1686567-1686586 MS.gene22853:intron 25.0%
! TTACTATTGCTAAATGAGTA+GGG + chr7.4:1686144-1686163 None:intergenic 25.0%
!! TTGTTAACTGTTACTTCAAT+GGG - chr7.4:1686092-1686111 MS.gene22853:CDS 25.0%
!! TTTGAGATAATCTGTAATGT+TGG - chr7.4:1686190-1686209 MS.gene22853:intron 25.0%
!!! GATTTCCTACATTTTTTGAA+AGG - chr7.4:1685675-1685694 MS.gene22853:intron 25.0%
!!! GATTTTATTTGTTCATCTGT+TGG - chr7.4:1686031-1686050 MS.gene22853:intron 25.0%
!!! GCATAATTTTAAAATTGTCG+CGG - chr7.4:1684960-1684979 MS.gene22853:CDS 25.0%
ACATAATATTTGGATGGGAA+AGG + chr7.4:1686690-1686709 None:intergenic 30.0%
AGATAATCTGTAATGTTGGT+CGG - chr7.4:1686194-1686213 MS.gene22853:intron 30.0%
AGGGAAATAAAAAATTGGGT+GGG + chr7.4:1685230-1685249 None:intergenic 30.0%
AGTGGAGGGAAATAAAAAAT+TGG + chr7.4:1685235-1685254 None:intergenic 30.0%
ATGAAGTAGCAGAAAAAGTA+AGG + chr7.4:1685925-1685944 None:intergenic 30.0%
ATTGGAACAAGAGATAACTT+TGG - chr7.4:1685797-1685816 MS.gene22853:intron 30.0%
CAACACCTTTCAAAAAATGT+AGG + chr7.4:1685683-1685702 None:intergenic 30.0%
CCAAATATTATGTCGTTACA+TGG - chr7.4:1686697-1686716 MS.gene22853:intron 30.0%
CCATGTAACGACATAATATT+TGG + chr7.4:1686700-1686719 None:intergenic 30.0%
CTATGACTTTCTTGTATAGA+TGG + chr7.4:1685776-1685795 None:intergenic 30.0%
GTAACGACATAATATTTGGA+TGG + chr7.4:1686696-1686715 None:intergenic 30.0%
GTGGAGGGAAATAAAAAATT+GGG + chr7.4:1685234-1685253 None:intergenic 30.0%
TACTATTGCTAAATGAGTAG+GGG + chr7.4:1686143-1686162 None:intergenic 30.0%
TCAGAATGATCTAGATAAAC+TGG + chr7.4:1687018-1687037 None:intergenic 30.0%
TCCTTGAAGCGAATTATATT+CGG + chr7.4:1686005-1686024 None:intergenic 30.0%
! ATAGGGATAATTAACTTGGA+AGG - chr7.4:1686813-1686832 MS.gene22853:intron 30.0%
! GTTGTTAACTGTTACTTCAA+TGG - chr7.4:1686091-1686110 MS.gene22853:CDS 30.0%
! TGTTAACTGTTACTTCAATG+GGG - chr7.4:1686093-1686112 MS.gene22853:CDS 30.0%
!! AAGTGATAGTTCTAATAGAG+TGG - chr7.4:1687068-1687087 MS.gene22853:CDS 30.0%
!! TATCACTTTTATGAGTAAGC+TGG + chr7.4:1687056-1687075 None:intergenic 30.0%
!!! TTTTTGAAAGGTGTTGTTTC+TGG - chr7.4:1685687-1685706 MS.gene22853:intron 30.0%
AAATTGACTGCAATGACAGT+AGG - chr7.4:1686449-1686468 MS.gene22853:intron 35.0%
AGGTAGACTAAATTTGGTTG+AGG + chr7.4:1685167-1685186 None:intergenic 35.0%
ATTATGGTTGATCACCTTCA+TGG - chr7.4:1686778-1686797 MS.gene22853:intron 35.0%
CAGTAGGTAATTCTTACCAT+AGG - chr7.4:1686465-1686484 MS.gene22853:intron 35.0%
CCATATCACAACGACATAAA+TGG + chr7.4:1685004-1685023 None:intergenic 35.0%
CCATTTATGTCGTTGTGATA+TGG - chr7.4:1685001-1685020 MS.gene22853:CDS 35.0%
CCTTGAAGCGAATTATATTC+GGG + chr7.4:1686004-1686023 None:intergenic 35.0%
CTGCAGAACTACTAATGTAT+TGG + chr7.4:1685739-1685758 None:intergenic 35.0%
GAAGATGAGGTTACTTCAAT+GGG - chr7.4:1686223-1686242 MS.gene22853:intron 35.0%
GAATATAATTCGCTTCAAGG+AGG - chr7.4:1686004-1686023 MS.gene22853:intron 35.0%
GACTTTCTTGTATAGATGGT+TGG + chr7.4:1685772-1685791 None:intergenic 35.0%
GAGGGAAATAAAAAATTGGG+TGG + chr7.4:1685231-1685250 None:intergenic 35.0%
GGCGGTAAGAAAAATATTTC+TGG + chr7.4:1686908-1686927 None:intergenic 35.0%
GGGAAATAAAAAATTGGGTG+GGG + chr7.4:1685229-1685248 None:intergenic 35.0%
GTATGTGTCCAATGGAATTA+TGG - chr7.4:1686762-1686781 MS.gene22853:intron 35.0%
GTGATCAACCATAATTCCAT+TGG + chr7.4:1686773-1686792 None:intergenic 35.0%
TAAAGAACAATAGACTCGTG+AGG - chr7.4:1685414-1685433 MS.gene22853:intron 35.0%
TAGAACGAACGTAAAACTCA+AGG - chr7.4:1686663-1686682 MS.gene22853:intron 35.0%
TCAAGGATGAAACTCATTCT+TGG - chr7.4:1685591-1685610 MS.gene22853:intron 35.0%
TTGCCATATTATGTGTGTGT+GGG + chr7.4:1685282-1685301 None:intergenic 35.0%
TTTCCAGATTGATGAGAATG+TGG - chr7.4:1685944-1685963 MS.gene22853:intron 35.0%
! ATGTGTTTTCGTGAGTCTTT+TGG + chr7.4:1685390-1685409 None:intergenic 35.0%
! GATAATTAACTTGGAAGGCT+AGG - chr7.4:1686818-1686837 MS.gene22853:intron 35.0%
!! AAAGGTGTTGTTTCTGGTAT+TGG - chr7.4:1685693-1685712 MS.gene22853:intron 35.0%
!! TACTTCAATGGGGTTTTGAT+TGG - chr7.4:1686103-1686122 MS.gene22853:CDS 35.0%
!! TCCTACTTTTGCATGAAAGT+CGG + chr7.4:1686929-1686948 None:intergenic 35.0%
!! TGATAGTTCTAATAGAGTGG+AGG - chr7.4:1687071-1687090 MS.gene22853:CDS 35.0%
!! TTTCAGGAAAGCGTAGATTT+TGG - chr7.4:1687103-1687122 MS.gene22853:CDS 35.0%
!!! AAGATGTTTTAGTGCTCTAG+TGG + chr7.4:1686863-1686882 None:intergenic 35.0%
!!! GCTTTTGGGAAAGTGTTTTA+TGG - chr7.4:1686261-1686280 MS.gene22853:intron 35.0%
ACTCTGCTTATACTGCAAGA+AGG + chr7.4:1686299-1686318 None:intergenic 40.0%
AGTGCAATTCGAGTATCATC+GGG + chr7.4:1686600-1686619 None:intergenic 40.0%
ATATTCGAACAGGTGCACAA+GGG - chr7.4:1686989-1687008 MS.gene22853:intron 40.0%
ATATTGCTTTGTCGCCATGA+AGG + chr7.4:1686795-1686814 None:intergenic 40.0%
ATGAGTCGTTGTATAGAGAC+TGG + chr7.4:1686427-1686446 None:intergenic 40.0%
CAGTGCAATTCGAGTATCAT+CGG + chr7.4:1686601-1686620 None:intergenic 40.0%
CATGGCGACAAAGCAATATA+GGG - chr7.4:1686796-1686815 MS.gene22853:intron 40.0%
CCCGAATATAATTCGCTTCA+AGG - chr7.4:1686001-1686020 MS.gene22853:intron 40.0%
CGAAGATGAGGTTACTTCAA+TGG - chr7.4:1686222-1686241 MS.gene22853:intron 40.0%
CTAATAGAGTGGAGGCAATT+AGG - chr7.4:1687079-1687098 MS.gene22853:CDS 40.0%
CTAGAGAGATTATCAACCAC+AGG - chr7.4:1685967-1685986 MS.gene22853:intron 40.0%
CTTGCCATATTATGTGTGTG+TGG + chr7.4:1685283-1685302 None:intergenic 40.0%
GATCAAAGCCATATTCGAAC+AGG - chr7.4:1686979-1686998 MS.gene22853:intron 40.0%
GGAACAAGAGATAACTTTGG+TGG - chr7.4:1685800-1685819 MS.gene22853:intron 40.0%
GGGAAGAGGTAGACTAAATT+TGG + chr7.4:1685173-1685192 None:intergenic 40.0%
TAATTCGCTTCAAGGAGGTA+AGG - chr7.4:1686009-1686028 MS.gene22853:intron 40.0%
TAGCCACATTCTCATCAATC+TGG + chr7.4:1685950-1685969 None:intergenic 40.0%
TCATGGCGACAAAGCAATAT+AGG - chr7.4:1686795-1686814 MS.gene22853:intron 40.0%
TTATCAACCACAGGTCTCTT+CGG - chr7.4:1685976-1685995 MS.gene22853:intron 40.0%
! ACGACTCACATCAGGAATTT+TGG + chr7.4:1686404-1686423 None:intergenic 40.0%
! ATAGTAATGGAGTATGCAGC+TGG - chr7.4:1686157-1686176 MS.gene22853:intron 40.0%
! TACTTTTGCATGAAAGTCGG+CGG + chr7.4:1686926-1686945 None:intergenic 40.0%
! TGTCGTTGTGATATGGATTG+TGG - chr7.4:1685008-1685027 MS.gene22853:CDS 40.0%
!! AGGAAAGCGTAGATTTTGGA+GGG - chr7.4:1687107-1687126 MS.gene22853:CDS 40.0%
!! TTATACTGCAAGAAGGTGCT+AGG + chr7.4:1686292-1686311 None:intergenic 40.0%
CATATTCGAACAGGTGCACA+AGG - chr7.4:1686988-1687007 MS.gene22853:intron 45.0%
CTTGTGCACCTGTTCGAATA+TGG + chr7.4:1686990-1687009 None:intergenic 45.0%
GGCTAGGATGAATATTGCAG+TGG - chr7.4:1686834-1686853 MS.gene22853:intron 45.0%
GTCTACGAGTATGTGTCCAA+TGG - chr7.4:1686754-1686773 MS.gene22853:intron 45.0%
TAATGGAGTATGCAGCTGGA+GGG - chr7.4:1686161-1686180 MS.gene22853:intron 45.0%
! GATATGGATTGTGGATGCTG+CGG - chr7.4:1685017-1685036 MS.gene22853:CDS 45.0%
! GCCGACTTTCATGCAAAAGT+AGG - chr7.4:1686925-1686944 MS.gene22853:intron 45.0%
! GTGGAGGCAATTAGGTTTTC+AGG - chr7.4:1687087-1687106 MS.gene22853:CDS 45.0%
!! CAGGAAAGCGTAGATTTTGG+AGG - chr7.4:1687106-1687125 MS.gene22853:CDS 45.0%
!! GGTTACTTCAATGGGCTACA+AGG - chr7.4:1686231-1686250 MS.gene22853:intron 45.0%
!!! CAATGGGGTTTTGATTGGTC+AGG - chr7.4:1686108-1686127 MS.gene22853:CDS 45.0%
!! TTTGTTTAAATAATTATTAA+GGG + chr7.4:1685194-1685213 None:intergenic 5.0%
!!! TTTTGTTTAAATAATTATTA+AGG + chr7.4:1685195-1685214 None:intergenic 5.0%
AACTAACAGCTGCGAGAGCA+TGG - chr7.4:1685565-1685584 MS.gene22853:intron 50.0%
AATGGAGTATGCAGCTGGAG+GGG - chr7.4:1686162-1686181 MS.gene22853:intron 50.0%
GCGCCCACACACACATAATA+TGG - chr7.4:1685276-1685295 MS.gene22853:intron 50.0%
! CTGCGAGAGCATGGAATTCA+AGG - chr7.4:1685574-1685593 MS.gene22853:intron 50.0%
! GTAATGGAGTATGCAGCTGG+AGG - chr7.4:1686160-1686179 MS.gene22853:intron 50.0%
! TACAAGGAGCAGGGGCTTTT+GGG - chr7.4:1686247-1686266 MS.gene22853:intron 50.0%
!! TCAATGGGCTACAAGGAGCA+GGG - chr7.4:1686238-1686257 MS.gene22853:intron 50.0%
!! TTCAATGGGCTACAAGGAGC+AGG - chr7.4:1686237-1686256 MS.gene22853:intron 50.0%
!!! TGGGGTTTTGATTGGTCAGG+TGG - chr7.4:1686111-1686130 MS.gene22853:CDS 50.0%
GAGACTGGACGACTCACATC+AGG + chr7.4:1686412-1686431 None:intergenic 55.0%
TGCGCGTGTGTGTTAGTGGA+GGG + chr7.4:1685249-1685268 None:intergenic 55.0%
TTACATGGGTGCACCTCACG+TGG - chr7.4:1686712-1686731 MS.gene22853:intron 55.0%
! CTACAAGGAGCAGGGGCTTT+TGG - chr7.4:1686246-1686265 MS.gene22853:intron 55.0%
! TGGTCGGTTCAGCGAAGATG+AGG - chr7.4:1686210-1686229 MS.gene22853:intron 55.0%
!! CAATGGGCTACAAGGAGCAG+GGG - chr7.4:1686239-1686258 MS.gene22853:intron 55.0%
CGCGGATGCACTTGCAGTTG+TGG - chr7.4:1684978-1684997 MS.gene22853:CDS 60.0%
CGTGTGCGCGTGTGTGTTAG+TGG + chr7.4:1685253-1685272 None:intergenic 60.0%
GTAACCGCGACTTCCACGTG+AGG + chr7.4:1686728-1686747 None:intergenic 60.0%
GTGCGCGTGTGTGTTAGTGG+AGG + chr7.4:1685250-1685269 None:intergenic 60.0%
TGCACCTCACGTGGAAGTCG+CGG - chr7.4:1686721-1686740 MS.gene22853:intron 60.0%
!! TCGGGTGCCGAAGAGACCTG+TGG + chr7.4:1685986-1686005 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 1684958 1687134 1684958 ID=MS.gene22853
chr7.4 mRNA 1684958 1687134 1684958 ID=MS.gene22853.t1;Parent=MS.gene22853
chr7.4 exon 1687037 1687134 1687037 ID=MS.gene22853.t1.exon1;Parent=MS.gene22853.t1
chr7.4 CDS 1687037 1687134 1687037 ID=cds.MS.gene22853.t1;Parent=MS.gene22853.t1
chr7.4 exon 1686480 1686518 1686480 ID=MS.gene22853.t1.exon2;Parent=MS.gene22853.t1
chr7.4 CDS 1686480 1686518 1686480 ID=cds.MS.gene22853.t1;Parent=MS.gene22853.t1
chr7.4 exon 1686269 1686359 1686269 ID=MS.gene22853.t1.exon3;Parent=MS.gene22853.t1
chr7.4 CDS 1686269 1686359 1686269 ID=cds.MS.gene22853.t1;Parent=MS.gene22853.t1
chr7.4 exon 1686067 1686141 1686067 ID=MS.gene22853.t1.exon4;Parent=MS.gene22853.t1
chr7.4 CDS 1686067 1686141 1686067 ID=cds.MS.gene22853.t1;Parent=MS.gene22853.t1
chr7.4 exon 1685810 1685962 1685810 ID=MS.gene22853.t1.exon5;Parent=MS.gene22853.t1
chr7.4 CDS 1685810 1685962 1685810 ID=cds.MS.gene22853.t1;Parent=MS.gene22853.t1
chr7.4 exon 1684958 1685257 1684958 ID=MS.gene22853.t1.exon6;Parent=MS.gene22853.t1
chr7.4 CDS 1684958 1685257 1684958 ID=cds.MS.gene22853.t1;Parent=MS.gene22853.t1
Gene Sequence

>MS.gene22853

ATGCATAATTTTAAAATTGTCGCGGATGCACTTGCAGTTGTGGCCATTTATGTCGTTGTGATATGGATTGTGGATGCTGCGGCGAAAAAAAATGTTCTGTAATTGTTATAGTTTTTATACATATTTTATATATAATTTTGAATATATTTTTAATTCATTTTAGAATATTCTATCATATTTATGAATTTATAAATATTTTAAATTTACCTCAACCAAATTTAGTCTACCTCTTCCCCTTAATAATTATTTAAACAAAATTTGCAACTCTCCCCACCCAATTTTTTATTTCCCTCCACTAACACACACGCGCACACGCGCGCGCCCACACACACATAATATGGCAAGAGCAAACATTTAATTAGACTTATGAAATAAGACTCAAATGTATATTATATAGATAAAATATCAACCTTAACAATTTTATTGATTCCAAAAGACTCACGAAAACACATCTCTTAAAGAACAATAGACTCGTGAGGAGATGATTCGTTCATCGTAAATGTTTAACAATACTCTTATGTTGAATTAGTTTACCACATATTTTTTGGTTGTTTATGCTAAACTAGTTTATTGAATCATCATAATGCTAACTCATCATTGCTTTCACAACTAACAGCTGCGAGAGCATGGAATTCAAGGATGAAACTCATTCTTGGTAATAAATCCAATTTTGATATTTTTCATGTAAACACTTTATTAACATAATATACTGATTTTGATTTCCTACATTTTTTGAAAGGTGTTGTTTCTGGTATTGGCGTCTATATTTATATAGGCGCCAATACATTAGTAGTTCTGCAGAGTCTCTCAACCAACCATCTATACAAGAAAGTCATAGAATTGGAACAAGAGATAACTTTGGTGGAGTTCACCATTTCACTTTTTAAAAAATATAATTAAAAAGAAAAAGTAGAAGAAGAAAAAAACTATTATTTTGACATCTGGGATTATAAGTTTTTTGTTTCCTTACTTTTTCTGCTACTTCATTTCCAGATTGATGAGAATGTGGCTAGAGAGATTATCAACCACAGGTCTCTTCGGCACCCGAATATAATTCGCTTCAAGGAGGTAAGGATTTTATTTGTTCATCTGTTGGATCAGTTTGTATTGAGTACATTGTTAATTGATTCTGAGTTGTTAACTGTTACTTCAATGGGGTTTTGATTGGTCAGGTGGTTTTGACCCCTACTCATTTAGCAATAGTAATGGAGTATGCAGCTGGAGGGGAACTCTTTGAGATAATCTGTAATGTTGGTCGGTTCAGCGAAGATGAGGTTACTTCAATGGGCTACAAGGAGCAGGGGCTTTTGGGAAAGTGTTTTATGGTAAAACCTAGCACCTTCTTGCAGTATAAGCAGAGTCCTTACTGTCAATAGTCATTATAGACAGTGATCATGTCCATATAAAAAGATCAAACAATTAAGATTATGAATTTTCAATTGGCCAAAATTCCTGATGTGAGTCGTCCAGTCTCTATACAACGACTCATATAAATTGACTGCAATGACAGTAGGTAATTCTTACCATAGGAAATTGAGTATGTTTTTGTTTATTGTATCTATTTCTACTTTACTTTTTAACATTCAATAATGTTAACTTATATTTTCTATCATATGATATTTCTTGTCTAGGTAAATTGCCCGATGATACTCGAATTGCACTGAAGAAGCTTCGTGATCTCTGCTTTGACACATATGATATATTTTAGAACGAACGTAAAACTCAAGGACCTTTCCCATCCAAATATTATGTCGTTACATGGGTGCACCTCACGTGGAAGTCGCGGTTACATGCTTGTCTACGAGTATGTGTCCAATGGAATTATGGTTGATCACCTTCATGGCGACAAAGCAATATAGGGATAATTAACTTGGAAGGCTAGGATGAATATTGCAGTGGAGACCACTAGAGCACTAAAACATCTTCATGCTTCTAACGTCATTAAAACCAGAAATATTTTTCTTACCGCCGACTTTCATGCAAAAGTAGGAGATTCTAGACTCTCTCATGATCTTCTGACTGATCAAAGCCATATTCGAACAGGTGCACAAGGGACACCAGTTTATCTAGATCATTCTGAGTACGATCAGTACAACCAGCTTACTCATAAAAGTGATAGTTCTAATAGAGTGGAGGCAATTAGGTTTTCAGGAAAGCGTAGATTTTGGAGGGAATAA
Protein sequence

>MS.gene22853.t1

MHNFKIVADALAVVAIYVVVIWIVDAAAKKNVLCESMEFKDETHSWRQYISSSAESLNQPSIQESHRIGTRDNFGGIDENVAREIINHRSLRHPNIIRFKEVVLTPTHLAIVMEYAAGGELFEIICNVGRFSEDEVTSMGYKEQGLLGKCFMARMNIAVETTRALKHLHASNVIKTRNIFLTADFHAKVGDSRLSHDLLTDQSHIRTGAQGTPVYLDHSEYDQYNQLTHKSDSSNRVEAIRFSGKRRFWRE