Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22935.t1 | XP_003591253.1 | 94.9 | 156 | 8 | 0 | 1 | 156 | 1 | 156 | 7.10E-74 | 286.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22935.t1 | Q9FJK3 | 46.8 | 156 | 82 | 1 | 1 | 155 | 1 | 156 | 1.8e-30 | 133.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22935.t1 | G7IAC3 | 94.9 | 156 | 8 | 0 | 1 | 156 | 1 | 156 | 5.1e-74 | 286.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene22935.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22935.t1 | MTR_1g084950 | 94.872 | 156 | 8 | 0 | 1 | 156 | 1 | 156 | 3.65e-99 | 282 |
| MS.gene22935.t1 | MTR_5g047580 | 85.897 | 156 | 22 | 0 | 1 | 156 | 1 | 156 | 9.00e-95 | 271 |
| MS.gene22935.t1 | MTR_7g011950 | 78.205 | 156 | 34 | 0 | 1 | 156 | 1 | 156 | 1.82e-79 | 232 |
| MS.gene22935.t1 | MTR_5g047560 | 76.282 | 156 | 21 | 1 | 1 | 156 | 1 | 140 | 1.26e-78 | 229 |
| MS.gene22935.t1 | MTR_4g028720 | 76.923 | 156 | 36 | 0 | 1 | 156 | 1 | 156 | 5.04e-78 | 229 |
| MS.gene22935.t1 | MTR_1g077320 | 75.641 | 156 | 38 | 0 | 1 | 156 | 1 | 156 | 2.01e-77 | 227 |
| MS.gene22935.t1 | MTR_1g077390 | 69.231 | 156 | 48 | 0 | 1 | 156 | 1 | 156 | 3.03e-75 | 221 |
| MS.gene22935.t1 | MTR_1g077300 | 73.718 | 156 | 39 | 1 | 1 | 156 | 1 | 154 | 1.11e-68 | 205 |
| MS.gene22935.t1 | MTR_1g090783 | 63.399 | 153 | 56 | 0 | 3 | 155 | 2 | 154 | 4.20e-65 | 196 |
| MS.gene22935.t1 | MTR_1g090697 | 59.355 | 155 | 63 | 0 | 1 | 155 | 1 | 155 | 1.96e-63 | 192 |
| MS.gene22935.t1 | MTR_1g090710 | 58.333 | 156 | 64 | 1 | 1 | 155 | 1 | 156 | 6.29e-62 | 188 |
| MS.gene22935.t1 | MTR_3g466980 | 46.104 | 154 | 82 | 1 | 1 | 153 | 1 | 154 | 2.26e-42 | 140 |
| MS.gene22935.t1 | MTR_3g065100 | 50.641 | 156 | 76 | 1 | 1 | 155 | 1 | 156 | 6.72e-42 | 140 |
| MS.gene22935.t1 | MTR_4g032620 | 39.355 | 155 | 92 | 2 | 1 | 153 | 1 | 155 | 1.80e-40 | 135 |
| MS.gene22935.t1 | MTR_4g063790 | 41.558 | 154 | 88 | 2 | 1 | 152 | 1 | 154 | 2.70e-36 | 123 |
| MS.gene22935.t1 | MTR_8g036130 | 40.881 | 159 | 90 | 2 | 1 | 155 | 1 | 159 | 5.58e-35 | 122 |
| MS.gene22935.t1 | MTR_5g075380 | 37.821 | 156 | 96 | 1 | 1 | 155 | 1 | 156 | 2.74e-34 | 118 |
| MS.gene22935.t1 | MTR_4g031910 | 42.308 | 156 | 88 | 2 | 1 | 154 | 1 | 156 | 1.14e-33 | 118 |
| MS.gene22935.t1 | MTR_3g466830 | 41.558 | 154 | 89 | 1 | 3 | 155 | 2 | 155 | 1.90e-33 | 115 |
| MS.gene22935.t1 | MTR_3g031240 | 36.306 | 157 | 98 | 2 | 1 | 155 | 1 | 157 | 1.13e-30 | 109 |
| MS.gene22935.t1 | MTR_3g466890 | 39.610 | 154 | 92 | 1 | 3 | 155 | 2 | 155 | 1.38e-30 | 108 |
| MS.gene22935.t1 | MTR_3g031100 | 36.943 | 157 | 97 | 2 | 1 | 155 | 1 | 157 | 2.09e-30 | 110 |
| MS.gene22935.t1 | MTR_4g032290 | 43.590 | 156 | 86 | 2 | 1 | 154 | 1 | 156 | 3.78e-30 | 109 |
| MS.gene22935.t1 | MTR_4g032260 | 42.949 | 156 | 87 | 2 | 1 | 154 | 1 | 156 | 4.80e-30 | 107 |
| MS.gene22935.t1 | MTR_3g467080 | 35.443 | 158 | 98 | 3 | 1 | 155 | 1 | 157 | 1.37e-28 | 103 |
| MS.gene22935.t1 | MTR_2g035610 | 44.628 | 121 | 66 | 1 | 1 | 120 | 1 | 121 | 1.44e-28 | 102 |
| MS.gene22935.t1 | MTR_2g016210 | 37.748 | 151 | 92 | 2 | 1 | 149 | 1 | 151 | 3.46e-28 | 104 |
| MS.gene22935.t1 | MTR_2g035580 | 34.194 | 155 | 101 | 1 | 1 | 154 | 1 | 155 | 4.33e-22 | 86.7 |
| MS.gene22935.t1 | MTR_3g465410 | 35.714 | 126 | 79 | 2 | 29 | 152 | 1 | 126 | 1.03e-21 | 85.5 |
| MS.gene22935.t1 | MTR_4g028800 | 48.649 | 74 | 36 | 1 | 1 | 74 | 1 | 72 | 7.76e-21 | 81.3 |
| MS.gene22935.t1 | MTR_3g466900 | 36.800 | 125 | 78 | 1 | 32 | 155 | 2 | 126 | 1.36e-20 | 82.4 |
| MS.gene22935.t1 | MTR_4g019670 | 37.903 | 124 | 69 | 3 | 1 | 120 | 1 | 120 | 2.53e-19 | 84.0 |
| MS.gene22935.t1 | MTR_1g033930 | 46.237 | 93 | 49 | 1 | 64 | 155 | 22 | 114 | 7.25e-19 | 77.4 |
| MS.gene22935.t1 | MTR_3g466930 | 34.400 | 125 | 81 | 1 | 32 | 155 | 2 | 126 | 8.34e-19 | 77.8 |
| MS.gene22935.t1 | MTR_7g106510 | 37.168 | 113 | 67 | 2 | 1 | 109 | 1 | 113 | 3.81e-18 | 80.5 |
| MS.gene22935.t1 | MTR_1g033370 | 45.000 | 100 | 54 | 1 | 57 | 155 | 21 | 120 | 3.40e-17 | 73.2 |
| MS.gene22935.t1 | MTR_7g055800 | 33.898 | 118 | 51 | 2 | 1 | 118 | 1 | 91 | 3.99e-16 | 69.7 |
| MS.gene22935.t1 | MTR_7g055790 | 42.727 | 110 | 46 | 2 | 1 | 110 | 59 | 151 | 4.24e-16 | 71.2 |
| MS.gene22935.t1 | MTR_1g033790 | 45.556 | 90 | 48 | 1 | 64 | 152 | 22 | 111 | 4.89e-16 | 70.1 |
| MS.gene22935.t1 | MTR_6g005450 | 31.818 | 110 | 69 | 3 | 1 | 104 | 1 | 110 | 2.12e-13 | 66.6 |
| MS.gene22935.t1 | MTR_3g093900 | 28.571 | 133 | 89 | 2 | 3 | 134 | 6 | 133 | 3.10e-13 | 66.2 |
| MS.gene22935.t1 | MTR_6g018920 | 29.091 | 110 | 72 | 3 | 1 | 104 | 1 | 110 | 3.50e-12 | 63.2 |
| MS.gene22935.t1 | MTR_1g114730 | 28.182 | 110 | 73 | 2 | 1 | 104 | 1 | 110 | 2.34e-11 | 60.8 |
| MS.gene22935.t1 | MTR_7g055940 | 32.692 | 104 | 43 | 2 | 1 | 104 | 1 | 77 | 3.00e-11 | 56.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22935.t1 | AT5G26630 | 44.872 | 156 | 85 | 1 | 1 | 155 | 1 | 156 | 8.81e-38 | 129 |
| MS.gene22935.t1 | AT5G48670 | 46.795 | 156 | 82 | 1 | 1 | 155 | 1 | 156 | 2.39e-35 | 125 |
| MS.gene22935.t1 | AT3G05860 | 40.268 | 149 | 88 | 1 | 1 | 148 | 1 | 149 | 4.37e-33 | 116 |
| MS.gene22935.t1 | AT1G65330 | 40.909 | 154 | 90 | 1 | 3 | 155 | 2 | 155 | 5.52e-33 | 118 |
| MS.gene22935.t1 | AT3G05860 | 40.268 | 149 | 88 | 1 | 1 | 148 | 1 | 149 | 1.78e-32 | 115 |
| MS.gene22935.t1 | AT3G05860 | 40.268 | 149 | 88 | 1 | 1 | 148 | 1 | 149 | 2.71e-32 | 115 |
| MS.gene22935.t1 | AT1G65300 | 38.312 | 154 | 94 | 1 | 3 | 155 | 2 | 155 | 3.28e-31 | 113 |
| MS.gene22935.t1 | AT5G26650 | 35.948 | 153 | 97 | 1 | 4 | 155 | 2 | 154 | 2.71e-27 | 104 |
| MS.gene22935.t1 | AT5G27960 | 34.641 | 153 | 99 | 1 | 4 | 155 | 2 | 154 | 3.46e-25 | 98.6 |
| MS.gene22935.t1 | AT1G31630 | 30.464 | 151 | 103 | 2 | 3 | 152 | 2 | 151 | 1.80e-24 | 97.1 |
| MS.gene22935.t1 | AT1G22590 | 32.051 | 156 | 102 | 2 | 1 | 155 | 1 | 153 | 3.17e-24 | 92.4 |
| MS.gene22935.t1 | AT1G31640 | 34.459 | 148 | 95 | 2 | 3 | 149 | 2 | 148 | 7.44e-23 | 93.6 |
| MS.gene22935.t1 | AT5G27810 | 44.444 | 90 | 49 | 1 | 29 | 117 | 1 | 90 | 7.67e-21 | 82.4 |
| MS.gene22935.t1 | AT2G28700 | 34.746 | 118 | 76 | 1 | 1 | 117 | 1 | 118 | 4.18e-20 | 85.1 |
| MS.gene22935.t1 | AT5G26580 | 32.903 | 155 | 91 | 2 | 1 | 155 | 1 | 142 | 1.73e-19 | 83.6 |
| MS.gene22935.t1 | AT5G06500 | 36.301 | 146 | 80 | 5 | 1 | 141 | 1 | 138 | 8.13e-18 | 77.8 |
| MS.gene22935.t1 | AT2G40210 | 34.884 | 129 | 81 | 2 | 1 | 127 | 1 | 128 | 4.95e-14 | 68.6 |
| MS.gene22935.t1 | AT5G55690 | 26.582 | 158 | 95 | 3 | 1 | 151 | 1 | 144 | 3.59e-12 | 62.8 |
| MS.gene22935.t1 | AT5G55690 | 26.582 | 158 | 95 | 3 | 1 | 151 | 1 | 144 | 3.59e-12 | 62.8 |
| MS.gene22935.t1 | AT1G47760 | 33.333 | 111 | 67 | 3 | 1 | 110 | 1 | 105 | 7.31e-12 | 60.8 |
| MS.gene22935.t1 | AT5G58890 | 34.234 | 111 | 65 | 3 | 1 | 104 | 1 | 110 | 9.76e-12 | 61.6 |
Find 25 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGAAGATGTTCCCTAGCTT+TGG | 0.376916 | 1.2:-58759493 | None:intergenic |
| GCCGGATGGTTTGACCGTTT+CGG | 0.379706 | 1.2:+58759587 | MS.gene22935:CDS |
| TGTTTAGCCTTCTCTAGATC+TGG | 0.403006 | 1.2:-58759387 | None:intergenic |
| CTTTGGTAATCCTCTGCAAT+AGG | 0.405146 | 1.2:-58759476 | None:intergenic |
| AGATCTAGAGAAGGCTAAAC+AGG | 0.406038 | 1.2:+58759389 | MS.gene22935:CDS |
| CTCTGATGATTCGGCAAGAA+AGG | 0.409977 | 1.2:+58759245 | MS.gene22935:CDS |
| TTTCACCTTCTTCCTAGTCA+TGG | 0.473113 | 1.2:-58759211 | None:intergenic |
| TCCGAAACGGTCAAACCATC+CGG | 0.476793 | 1.2:-58759588 | None:intergenic |
| TCTGCAATAGGAAGCTCTCT+TGG | 0.511328 | 1.2:-58759464 | None:intergenic |
| AGCTGGAATACCGCATAGAA+TGG | 0.529502 | 1.2:-58759319 | None:intergenic |
| TTGGAAATTATAGCACAAGC+TGG | 0.550429 | 1.2:-58759336 | None:intergenic |
| TTGCATTGCAAGCCATGACT+AGG | 0.554602 | 1.2:+58759199 | None:intergenic |
| GCAAGCCATGACTAGGAAGA+AGG | 0.558932 | 1.2:+58759206 | None:intergenic |
| AAACTCTTTCAAATCCGAAA+CGG | 0.563009 | 1.2:-58759601 | None:intergenic |
| TGCAGAGGATTACCAAAGCT+AGG | 0.568390 | 1.2:+58759481 | MS.gene22935:CDS |
| GCAGAGGATTACCAAAGCTA+GGG | 0.571791 | 1.2:+58759482 | MS.gene22935:CDS |
| GGGAACATCTTCAAAAGCAA+AGG | 0.603982 | 1.2:+58759502 | MS.gene22935:CDS |
| TGAGAGGTATCAAAATTCAT+CGG | 0.625826 | 1.2:+58759416 | MS.gene22935:CDS |
| AAGAGAGCTTCCTATTGCAG+AGG | 0.626560 | 1.2:+58759466 | MS.gene22935:CDS |
| AGGCTAAACAGGTGATTGAG+AGG | 0.629216 | 1.2:+58759400 | MS.gene22935:CDS |
| TTGATTCTAAAACAGAGGTG+TGG | 0.633348 | 1.2:+58759364 | MS.gene22935:CDS |
| AAGGCACGACAATCGTGAGA+AGG | 0.638977 | 1.2:+58759521 | MS.gene22935:CDS |
| GGTGTGGCCAGATCTAGAGA+AGG | 0.655354 | 1.2:+58759380 | MS.gene22935:CDS |
| GATAACAAGATTGATGCACT+CGG | 0.692256 | 1.2:+58759657 | MS.gene22935:CDS |
| AGCGAACTCACCATTCTATG+CGG | 0.710886 | 1.2:+58759309 | MS.gene22935:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACCTATAAGAAAAGAAAAA+AGG | + | chr1.2:58759269-58759288 | MS.gene22935:CDS | 20.0% |
| !! | ACCTATAAGAAAAGAAAAAA+GGG | + | chr1.2:58759270-58759289 | MS.gene22935:CDS | 20.0% |
| ! | GCTAATTGAGAAAAATATGA+AGG | + | chr1.2:58759629-58759648 | MS.gene22935:CDS | 25.0% |
| !!! | AAAAAAGGGTATCATCAAAA+AGG | + | chr1.2:58759284-58759303 | MS.gene22935:CDS | 25.0% |
| !!! | ACCCTTTTTTCTTTTCTTAT+AGG | - | chr1.2:58759274-58759293 | None:intergenic | 25.0% |
| !!! | TGTTTTAGAATCAAAAGGAT+TGG | - | chr1.2:58759358-58759377 | None:intergenic | 25.0% |
| AAACTCTTTCAAATCCGAAA+CGG | - | chr1.2:58759604-58759623 | None:intergenic | 30.0% | |
| ! | TGCTTTTATCTCTGATGATT+CGG | + | chr1.2:58759236-58759255 | MS.gene22935:CDS | 30.0% |
| !! | TGAGAGGTATCAAAATTCAT+CGG | + | chr1.2:58759416-58759435 | MS.gene22935:CDS | 30.0% |
| !!! | ACCTCTGTTTTAGAATCAAA+AGG | - | chr1.2:58759363-58759382 | None:intergenic | 30.0% |
| !!! | TCCTTTTGATTCTAAAACAG+AGG | + | chr1.2:58759359-58759378 | MS.gene22935:CDS | 30.0% |
| CATGAAAAACAAAAAGCTGC+CGG | + | chr1.2:58759569-58759588 | MS.gene22935:CDS | 35.0% | |
| GATAACAAGATTGATGCACT+CGG | + | chr1.2:58759657-58759676 | MS.gene22935:CDS | 35.0% | |
| TTGGAAATTATAGCACAAGC+TGG | - | chr1.2:58759339-58759358 | None:intergenic | 35.0% | |
| !! | TTGATTCTAAAACAGAGGTG+TGG | + | chr1.2:58759364-58759383 | MS.gene22935:CDS | 35.0% |
| AAAAACAAAAAGCTGCCGGA+TGG | + | chr1.2:58759573-58759592 | MS.gene22935:CDS | 40.0% | |
| AGATCTAGAGAAGGCTAAAC+AGG | + | chr1.2:58759389-58759408 | MS.gene22935:CDS | 40.0% | |
| GGGAACATCTTCAAAAGCAA+AGG | + | chr1.2:58759502-58759521 | MS.gene22935:CDS | 40.0% | |
| TTGAAGATGTTCCCTAGCTT+TGG | - | chr1.2:58759496-58759515 | None:intergenic | 40.0% | |
| ! | CTTTGGTAATCCTCTGCAAT+AGG | - | chr1.2:58759479-58759498 | None:intergenic | 40.0% |
| ! | TGTTTAGCCTTCTCTAGATC+TGG | - | chr1.2:58759390-58759409 | None:intergenic | 40.0% |
| AAGAGAGCTTCCTATTGCAG+AGG | + | chr1.2:58759466-58759485 | MS.gene22935:CDS | 45.0% | |
| AGCGAACTCACCATTCTATG+CGG | + | chr1.2:58759309-58759328 | MS.gene22935:CDS | 45.0% | |
| AGCTGGAATACCGCATAGAA+TGG | - | chr1.2:58759322-58759341 | None:intergenic | 45.0% | |
| AGGCTAAACAGGTGATTGAG+AGG | + | chr1.2:58759400-58759419 | MS.gene22935:CDS | 45.0% | |
| GCAGAGGATTACCAAAGCTA+GGG | + | chr1.2:58759482-58759501 | MS.gene22935:CDS | 45.0% | |
| TCTGCAATAGGAAGCTCTCT+TGG | - | chr1.2:58759467-58759486 | None:intergenic | 45.0% | |
| TGCAGAGGATTACCAAAGCT+AGG | + | chr1.2:58759481-58759500 | MS.gene22935:CDS | 45.0% | |
| ! | CTCTGATGATTCGGCAAGAA+AGG | + | chr1.2:58759245-58759264 | MS.gene22935:CDS | 45.0% |
| AAGGCACGACAATCGTGAGA+AGG | + | chr1.2:58759521-58759540 | MS.gene22935:CDS | 50.0% | |
| TCCGAAACGGTCAAACCATC+CGG | - | chr1.2:58759591-58759610 | None:intergenic | 50.0% | |
| GCCGGATGGTTTGACCGTTT+CGG | + | chr1.2:58759587-58759606 | MS.gene22935:CDS | 55.0% | |
| GGTGTGGCCAGATCTAGAGA+AGG | + | chr1.2:58759380-58759399 | MS.gene22935:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 58759213 | 58759683 | 58759213 | ID=MS.gene22935 |
| chr1.2 | mRNA | 58759213 | 58759683 | 58759213 | ID=MS.gene22935.t1;Parent=MS.gene22935 |
| chr1.2 | exon | 58759213 | 58759683 | 58759213 | ID=MS.gene22935.t1.exon1;Parent=MS.gene22935.t1 |
| chr1.2 | CDS | 58759213 | 58759683 | 58759213 | ID=cds.MS.gene22935.t1;Parent=MS.gene22935.t1 |
| Gene Sequence |
| Protein sequence |
