Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22949.t1 | KEH19403.1 | 67.9 | 137 | 36 | 2 | 12 | 148 | 18 | 146 | 8.40E-43 | 183.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22949.t1 | Q9FNG6 | 50.7 | 150 | 24 | 1 | 19 | 118 | 129 | 278 | 6.1e-34 | 145.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22949.t1 | A0A072U5J1 | 67.9 | 137 | 36 | 2 | 12 | 148 | 18 | 146 | 6.0e-43 | 183.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene22949.t1 | TF | C3H |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22949.t1 | MTR_7g108868 | 67.883 | 137 | 36 | 2 | 12 | 148 | 18 | 146 | 1.52e-60 | 185 |
| MS.gene22949.t1 | MTR_8g058593 | 67.883 | 137 | 36 | 2 | 12 | 148 | 18 | 146 | 1.52e-60 | 185 |
| MS.gene22949.t1 | MTR_8g058607 | 77.477 | 111 | 23 | 1 | 12 | 122 | 31 | 139 | 8.58e-59 | 179 |
| MS.gene22949.t1 | MTR_7g109250 | 50.000 | 192 | 28 | 3 | 1 | 125 | 50 | 240 | 1.28e-53 | 172 |
| MS.gene22949.t1 | MTR_7g109250 | 49.215 | 191 | 31 | 2 | 1 | 125 | 110 | 300 | 1.39e-52 | 171 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22949.t1 | AT5G06420 | 49.375 | 160 | 31 | 1 | 19 | 128 | 129 | 288 | 1.32e-45 | 153 |
| MS.gene22949.t1 | AT5G06420 | 49.375 | 160 | 31 | 1 | 19 | 128 | 129 | 288 | 1.32e-45 | 153 |
| MS.gene22949.t1 | AT1G01350 | 48.750 | 160 | 32 | 1 | 19 | 128 | 94 | 253 | 1.76e-44 | 149 |
Find 30 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAAGGGAAAAGGACTTTC+TGG | 0.207237 | 7.3:+6002564 | MS.gene22949:CDS |
| GCGCACACTCTTTGCTTTCT+CGG | 0.276894 | 7.3:-6002503 | None:intergenic |
| GACCGAGGCGACTACAATTC+CGG | 0.339363 | 7.3:+6002451 | MS.gene22949:CDS |
| TTGAAGTCTTTGCATATGTC+AGG | 0.387439 | 7.3:-6002382 | None:intergenic |
| TGACATATGCAAAGACTTCA+AGG | 0.411330 | 7.3:+6002384 | MS.gene22949:CDS |
| TGAAGACTTAAAATGAAAGA+TGG | 0.412793 | 7.3:-6002212 | None:intergenic |
| AAAATGAAAGATGGGCTTCT+CGG | 0.416615 | 7.3:-6002203 | None:intergenic |
| ACCGAGGCGACTACAATTCC+GGG | 0.427387 | 7.3:+6002452 | MS.gene22949:CDS |
| TAGCAAAGCAACAGCAACTT+TGG | 0.436558 | 7.3:+6002258 | MS.gene22949:CDS |
| TGATGAAACTCTAATATAAG+TGG | 0.474441 | 7.3:-6002344 | None:intergenic |
| TGCAAAGACTTCAAGGAGAC+CGG | 0.477457 | 7.3:+6002391 | MS.gene22949:CDS |
| GAAGACTTAAAATGAAAGAT+GGG | 0.487156 | 7.3:-6002211 | None:intergenic |
| GGTGGCAGTTGGACAAAGAA+TGG | 0.496757 | 7.3:+6002473 | MS.gene22949:CDS |
| AAAGAGTGTGCGCGCGCTGA+TGG | 0.507062 | 7.3:+6002513 | MS.gene22949:CDS |
| TTGTCTGTTTGTTTGTCTGA+AGG | 0.512605 | 7.3:+6002537 | MS.gene22949:CDS |
| TTCAAGGAGACCGGGTATTG+TGG | 0.545327 | 7.3:+6002400 | MS.gene22949:CDS |
| CTACAATTCCGGGTGGCAGT+TGG | 0.548426 | 7.3:+6002462 | MS.gene22949:CDS |
| ACCCGGAATTGTAGTCGCCT+CGG | 0.550971 | 7.3:-6002453 | None:intergenic |
| TTCTTTGTCCAACTGCCACC+CGG | 0.562902 | 7.3:-6002470 | None:intergenic |
| TGCAAGTTTCTTCACGACCG+AGG | 0.564969 | 7.3:+6002436 | MS.gene22949:CDS |
| GAGACCGGGTATTGTGGCTA+CGG | 0.578795 | 7.3:+6002406 | MS.gene22949:CDS |
| ATCGCCGTAGCCACAATACC+CGG | 0.586901 | 7.3:-6002410 | None:intergenic |
| GAAACTCTAATATAAGTGGA+AGG | 0.596767 | 7.3:-6002340 | None:intergenic |
| ATCCGTGGGTCACACTCACA+CGG | 0.602004 | 7.3:+6002307 | MS.gene22949:CDS |
| ACATGTAAATTACAAACACC+CGG | 0.625741 | 7.3:-6002605 | None:intergenic |
| GCAAAGACTTCAAGGAGACC+GGG | 0.633254 | 7.3:+6002392 | MS.gene22949:CDS |
| GTGTGAGTGTGACCCACGGA+TGG | 0.640412 | 7.3:-6002305 | None:intergenic |
| ATATAAGTGGAAGGTCTGAG+AGG | 0.681694 | 7.3:-6002331 | None:intergenic |
| GACCGTGTGAGTGTGACCCA+CGG | 0.684896 | 7.3:-6002309 | None:intergenic |
| GAGGCGACTACAATTCCGGG+TGG | 0.758896 | 7.3:+6002455 | MS.gene22949:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GAAGACTTAAAATGAAAGAT+GGG | - | chr7.3:6002214-6002233 | None:intergenic | 25.0% |
| ! | TGAAGACTTAAAATGAAAGA+TGG | - | chr7.3:6002215-6002234 | None:intergenic | 25.0% |
| ! | TGATGAAACTCTAATATAAG+TGG | - | chr7.3:6002347-6002366 | None:intergenic | 25.0% |
| GAAACTCTAATATAAGTGGA+AGG | - | chr7.3:6002343-6002362 | None:intergenic | 30.0% | |
| TGAGAAAAAGAACATGAAGA+AGG | + | chr7.3:6002162-6002181 | MS.gene22949:CDS | 30.0% | |
| TTGTCTGAAGGAAAAAAGAA+GGG | + | chr7.3:6002549-6002568 | MS.gene22949:CDS | 30.0% | |
| TTTGTCTGAAGGAAAAAAGA+AGG | + | chr7.3:6002548-6002567 | MS.gene22949:CDS | 30.0% | |
| GAAGGAAAAAAGAAGGGAAA+AGG | + | chr7.3:6002555-6002574 | MS.gene22949:CDS | 35.0% | |
| TGACATATGCAAAGACTTCA+AGG | + | chr7.3:6002384-6002403 | MS.gene22949:CDS | 35.0% | |
| TTGAAGTCTTTGCATATGTC+AGG | - | chr7.3:6002385-6002404 | None:intergenic | 35.0% | |
| TTGTCTGTTTGTTTGTCTGA+AGG | + | chr7.3:6002537-6002556 | MS.gene22949:CDS | 35.0% | |
| ! | AAAATGAAAGATGGGCTTCT+CGG | - | chr7.3:6002206-6002225 | None:intergenic | 35.0% |
| !! | ATTTTGCTGACTACAATTCC+GGG | + | chr7.3:6002587-6002606 | MS.gene22949:CDS | 35.0% |
| !! | GATTTTGCTGACTACAATTC+CGG | + | chr7.3:6002586-6002605 | MS.gene22949:CDS | 35.0% |
| !!! | ATTTTTCAAGTTCCATCCGT+GGG | + | chr7.3:6002293-6002312 | MS.gene22949:CDS | 35.0% |
| AAGAAGGGAAAAGGACTTTC+TGG | + | chr7.3:6002564-6002583 | MS.gene22949:CDS | 40.0% | |
| ATATAAGTGGAAGGTCTGAG+AGG | - | chr7.3:6002334-6002353 | None:intergenic | 40.0% | |
| TAGCAAAGCAACAGCAACTT+TGG | + | chr7.3:6002258-6002277 | MS.gene22949:CDS | 40.0% | |
| !!! | CATTTTTCAAGTTCCATCCG+TGG | + | chr7.3:6002292-6002311 | MS.gene22949:CDS | 40.0% |
| TGCAAAGACTTCAAGGAGAC+CGG | + | chr7.3:6002391-6002410 | MS.gene22949:CDS | 45.0% | |
| GCAAAGACTTCAAGGAGACC+GGG | + | chr7.3:6002392-6002411 | MS.gene22949:CDS | 50.0% | |
| GCGCACACTCTTTGCTTTCT+CGG | - | chr7.3:6002506-6002525 | None:intergenic | 50.0% | |
| GGTGGCAGTTGGACAAAGAA+TGG | + | chr7.3:6002473-6002492 | MS.gene22949:CDS | 50.0% | |
| TGCAAGTTTCTTCACGACCG+AGG | + | chr7.3:6002436-6002455 | MS.gene22949:CDS | 50.0% | |
| TTCTTTGTCCAACTGCCACC+CGG | - | chr7.3:6002473-6002492 | None:intergenic | 50.0% | |
| ! | TTCAAGGAGACCGGGTATTG+TGG | + | chr7.3:6002400-6002419 | MS.gene22949:CDS | 50.0% |
| ACCCGGAATTGTAGTCGCCT+CGG | - | chr7.3:6002456-6002475 | None:intergenic | 55.0% | |
| ACCGAGGCGACTACAATTCC+GGG | + | chr7.3:6002452-6002471 | MS.gene22949:CDS | 55.0% | |
| ATCCGTGGGTCACACTCACA+CGG | + | chr7.3:6002307-6002326 | MS.gene22949:CDS | 55.0% | |
| ATCGCCGTAGCCACAATACC+CGG | - | chr7.3:6002413-6002432 | None:intergenic | 55.0% | |
| CTACAATTCCGGGTGGCAGT+TGG | + | chr7.3:6002462-6002481 | MS.gene22949:CDS | 55.0% | |
| GACCGAGGCGACTACAATTC+CGG | + | chr7.3:6002451-6002470 | MS.gene22949:CDS | 55.0% | |
| ! | GAGACCGGGTATTGTGGCTA+CGG | + | chr7.3:6002406-6002425 | MS.gene22949:CDS | 55.0% |
| GACCGTGTGAGTGTGACCCA+CGG | - | chr7.3:6002312-6002331 | None:intergenic | 60.0% | |
| GAGGCGACTACAATTCCGGG+TGG | + | chr7.3:6002455-6002474 | MS.gene22949:CDS | 60.0% | |
| GTGTGAGTGTGACCCACGGA+TGG | - | chr7.3:6002308-6002327 | None:intergenic | 60.0% | |
| ! | AAAGAGTGTGCGCGCGCTGA+TGG | + | chr7.3:6002513-6002532 | MS.gene22949:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 6002151 | 6002618 | 6002151 | ID=MS.gene22949 |
| chr7.3 | mRNA | 6002151 | 6002618 | 6002151 | ID=MS.gene22949.t1;Parent=MS.gene22949 |
| chr7.3 | exon | 6002151 | 6002618 | 6002151 | ID=MS.gene22949.t1.exon1;Parent=MS.gene22949.t1 |
| chr7.3 | CDS | 6002151 | 6002618 | 6002151 | ID=cds.MS.gene22949.t1;Parent=MS.gene22949.t1 |
| Gene Sequence |
| Protein sequence |
