Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22950.t1 | KEH19406.1 | 80.5 | 113 | 20 | 1 | 4 | 116 | 31 | 141 | 5.70E-44 | 186.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22950.t1 | Q9FNG6 | 51.3 | 150 | 23 | 1 | 11 | 110 | 129 | 278 | 1.6e-34 | 146.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22950.t1 | A0A072TQE4 | 80.5 | 113 | 20 | 1 | 4 | 116 | 31 | 141 | 4.1e-44 | 186.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene22950.t1 | TF | C3H |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22950.t1 | MTR_8g058607 | 80.531 | 113 | 20 | 1 | 4 | 116 | 31 | 141 | 8.41e-63 | 188 |
| MS.gene22950.t1 | MTR_7g108868 | 80.180 | 111 | 20 | 1 | 4 | 114 | 18 | 126 | 1.15e-61 | 186 |
| MS.gene22950.t1 | MTR_8g058593 | 80.180 | 111 | 20 | 1 | 4 | 114 | 18 | 126 | 1.15e-61 | 186 |
| MS.gene22950.t1 | MTR_7g109250 | 55.696 | 158 | 20 | 1 | 7 | 114 | 80 | 237 | 6.61e-53 | 169 |
| MS.gene22950.t1 | MTR_7g109250 | 55.696 | 158 | 20 | 1 | 7 | 114 | 140 | 297 | 1.08e-51 | 167 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22950.t1 | AT5G06420 | 51.923 | 156 | 25 | 1 | 11 | 116 | 129 | 284 | 4.67e-47 | 155 |
| MS.gene22950.t1 | AT5G06420 | 51.923 | 156 | 25 | 1 | 11 | 116 | 129 | 284 | 4.67e-47 | 155 |
| MS.gene22950.t1 | AT1G01350 | 51.282 | 156 | 26 | 1 | 11 | 116 | 94 | 249 | 8.00e-46 | 151 |
Find 30 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTGAAGTCTTTGCATATGTC+AGG | 0.387439 | 7.3:-6003135 | None:intergenic |
| TCAGCGCACCCTCCTCTTCT+CGG | 0.409916 | 7.3:-6003256 | None:intergenic |
| TGACATATGCAAAGACTTCA+AGG | 0.411330 | 7.3:+6003137 | MS.gene22950:CDS |
| TGAAGACTTAAAATGAAAGA+TGG | 0.412793 | 7.3:-6002965 | None:intergenic |
| AAAATGAAAGATGGGCTTCT+CGG | 0.416615 | 7.3:-6002956 | None:intergenic |
| TAGCAAAGCAACAGCAACTT+TGG | 0.436558 | 7.3:+6003011 | MS.gene22950:CDS |
| GACCGAGGCGATTACAAGTC+CGG | 0.470020 | 7.3:+6003204 | MS.gene22950:CDS |
| TGATGAAACTCTAATATAAG+TGG | 0.474441 | 7.3:-6003097 | None:intergenic |
| TGCAAAGACTTCAAGGAGAC+CGG | 0.477457 | 7.3:+6003144 | MS.gene22950:CDS |
| GAAGACTTAAAATGAAAGAT+GGG | 0.487156 | 7.3:-6002964 | None:intergenic |
| GGTGGCAGTTGGACAAAGAA+TGG | 0.496757 | 7.3:+6003226 | MS.gene22950:CDS |
| TTCAAGGAGACCGGGTATTG+TGG | 0.545327 | 7.3:+6003153 | MS.gene22950:CDS |
| AGAAGAGGAGGGTGCGCTGA+TGG | 0.548558 | 7.3:+6003259 | MS.gene22950:CDS |
| TTCTTTGTCCAACTGCCACC+CGG | 0.548782 | 7.3:-6003223 | None:intergenic |
| ACCGAGGCGATTACAAGTCC+GGG | 0.556767 | 7.3:+6003205 | MS.gene22950:CDS |
| GGAATGAAGCCGAGAAGAGG+AGG | 0.558808 | 7.3:+6003247 | MS.gene22950:CDS |
| ACCCGGACTTGTAATCGCCT+CGG | 0.560050 | 7.3:-6003206 | None:intergenic |
| TTACAAGTCCGGGTGGCAGT+TGG | 0.560817 | 7.3:+6003215 | MS.gene22950:CDS |
| TGCAAGTTTCTTCACGACCG+AGG | 0.564969 | 7.3:+6003189 | MS.gene22950:CDS |
| GAGACCGGGTATTGTGGCTA+CGG | 0.578795 | 7.3:+6003159 | MS.gene22950:CDS |
| ATCGCCGTAGCCACAATACC+CGG | 0.586901 | 7.3:-6003163 | None:intergenic |
| AATGGAATGAAGCCGAGAAG+AGG | 0.595307 | 7.3:+6003244 | MS.gene22950:CDS |
| GAAACTCTAATATAAGTGGA+AGG | 0.596767 | 7.3:-6003093 | None:intergenic |
| ATCCGTGGGTCACACTCACA+CGG | 0.602004 | 7.3:+6003060 | MS.gene22950:CDS |
| GCAAAGACTTCAAGGAGACC+GGG | 0.633254 | 7.3:+6003145 | MS.gene22950:CDS |
| GTGTGAGTGTGACCCACGGA+TGG | 0.640412 | 7.3:-6003058 | None:intergenic |
| GAATGAAGCCGAGAAGAGGA+GGG | 0.646762 | 7.3:+6003248 | MS.gene22950:CDS |
| ATATAAGTGGAAGGTCTGAG+AGG | 0.681694 | 7.3:-6003084 | None:intergenic |
| GACCGTGTGAGTGTGACCCA+CGG | 0.684896 | 7.3:-6003062 | None:intergenic |
| GAGGCGATTACAAGTCCGGG+TGG | 0.724834 | 7.3:+6003208 | MS.gene22950:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GAAGACTTAAAATGAAAGAT+GGG | - | chr7.3:6002967-6002986 | None:intergenic | 25.0% |
| ! | TGAAGACTTAAAATGAAAGA+TGG | - | chr7.3:6002968-6002987 | None:intergenic | 25.0% |
| ! | TGATGAAACTCTAATATAAG+TGG | - | chr7.3:6003100-6003119 | None:intergenic | 25.0% |
| GAAACTCTAATATAAGTGGA+AGG | - | chr7.3:6003096-6003115 | None:intergenic | 30.0% | |
| TGACATATGCAAAGACTTCA+AGG | + | chr7.3:6003137-6003156 | MS.gene22950:CDS | 35.0% | |
| TTGAAGTCTTTGCATATGTC+AGG | - | chr7.3:6003138-6003157 | None:intergenic | 35.0% | |
| ! | AAAATGAAAGATGGGCTTCT+CGG | - | chr7.3:6002959-6002978 | None:intergenic | 35.0% |
| !!! | ATTTTTCAAGTTCCATCCGT+GGG | + | chr7.3:6003046-6003065 | MS.gene22950:CDS | 35.0% |
| ATATAAGTGGAAGGTCTGAG+AGG | - | chr7.3:6003087-6003106 | None:intergenic | 40.0% | |
| TAGCAAAGCAACAGCAACTT+TGG | + | chr7.3:6003011-6003030 | MS.gene22950:CDS | 40.0% | |
| !!! | CATTTTTCAAGTTCCATCCG+TGG | + | chr7.3:6003045-6003064 | MS.gene22950:CDS | 40.0% |
| AATGGAATGAAGCCGAGAAG+AGG | + | chr7.3:6003244-6003263 | MS.gene22950:CDS | 45.0% | |
| TGCAAAGACTTCAAGGAGAC+CGG | + | chr7.3:6003144-6003163 | MS.gene22950:CDS | 45.0% | |
| GAATGAAGCCGAGAAGAGGA+GGG | + | chr7.3:6003248-6003267 | MS.gene22950:CDS | 50.0% | |
| GCAAAGACTTCAAGGAGACC+GGG | + | chr7.3:6003145-6003164 | MS.gene22950:CDS | 50.0% | |
| GGTGGCAGTTGGACAAAGAA+TGG | + | chr7.3:6003226-6003245 | MS.gene22950:CDS | 50.0% | |
| TGCAAGTTTCTTCACGACCG+AGG | + | chr7.3:6003189-6003208 | MS.gene22950:CDS | 50.0% | |
| TTCTTTGTCCAACTGCCACC+CGG | - | chr7.3:6003226-6003245 | None:intergenic | 50.0% | |
| ! | TTCAAGGAGACCGGGTATTG+TGG | + | chr7.3:6003153-6003172 | MS.gene22950:CDS | 50.0% |
| ACCCGGACTTGTAATCGCCT+CGG | - | chr7.3:6003209-6003228 | None:intergenic | 55.0% | |
| ACCGAGGCGATTACAAGTCC+GGG | + | chr7.3:6003205-6003224 | MS.gene22950:CDS | 55.0% | |
| ATCCGTGGGTCACACTCACA+CGG | + | chr7.3:6003060-6003079 | MS.gene22950:CDS | 55.0% | |
| ATCGCCGTAGCCACAATACC+CGG | - | chr7.3:6003166-6003185 | None:intergenic | 55.0% | |
| GACCGAGGCGATTACAAGTC+CGG | + | chr7.3:6003204-6003223 | MS.gene22950:CDS | 55.0% | |
| GGAATGAAGCCGAGAAGAGG+AGG | + | chr7.3:6003247-6003266 | MS.gene22950:CDS | 55.0% | |
| TTACAAGTCCGGGTGGCAGT+TGG | + | chr7.3:6003215-6003234 | MS.gene22950:CDS | 55.0% | |
| ! | GAGACCGGGTATTGTGGCTA+CGG | + | chr7.3:6003159-6003178 | MS.gene22950:CDS | 55.0% |
| GACCGTGTGAGTGTGACCCA+CGG | - | chr7.3:6003065-6003084 | None:intergenic | 60.0% | |
| GAGGCGATTACAAGTCCGGG+TGG | + | chr7.3:6003208-6003227 | MS.gene22950:CDS | 60.0% | |
| GTGTGAGTGTGACCCACGGA+TGG | - | chr7.3:6003061-6003080 | None:intergenic | 60.0% | |
| TCAGCGCACCCTCCTCTTCT+CGG | - | chr7.3:6003259-6003278 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 6002928 | 6003278 | 6002928 | ID=MS.gene22950 |
| chr7.3 | mRNA | 6002928 | 6003278 | 6002928 | ID=MS.gene22950.t1;Parent=MS.gene22950 |
| chr7.3 | exon | 6002928 | 6003278 | 6002928 | ID=MS.gene22950.t1.exon1;Parent=MS.gene22950.t1 |
| chr7.3 | CDS | 6002928 | 6003278 | 6002928 | ID=cds.MS.gene22950.t1;Parent=MS.gene22950.t1 |
| Gene Sequence |
| Protein sequence |
