Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22975.t1 | KEH19403.1 | 80.7 | 109 | 19 | 1 | 4 | 112 | 18 | 124 | 4.60E-42 | 180.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22975.t1 | Q9FNG6 | 50.7 | 150 | 24 | 1 | 11 | 110 | 129 | 278 | 1.8e-34 | 146.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22975.t1 | A0A072U5J1 | 80.7 | 109 | 19 | 1 | 4 | 112 | 18 | 124 | 3.3e-42 | 180.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene22975.t1 | TF | C3H |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22975.t1 | MTR_7g108868 | 80.734 | 109 | 19 | 1 | 4 | 112 | 18 | 124 | 3.08e-60 | 184 |
| MS.gene22975.t1 | MTR_8g058593 | 80.734 | 109 | 19 | 1 | 4 | 112 | 18 | 124 | 3.08e-60 | 184 |
| MS.gene22975.t1 | MTR_8g058607 | 80.734 | 109 | 19 | 1 | 4 | 112 | 31 | 137 | 5.87e-60 | 181 |
| MS.gene22975.t1 | MTR_7g109250 | 55.414 | 157 | 20 | 1 | 7 | 113 | 80 | 236 | 2.12e-52 | 168 |
| MS.gene22975.t1 | MTR_7g109250 | 55.414 | 157 | 20 | 1 | 7 | 113 | 140 | 296 | 3.91e-51 | 166 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene22975.t1 | AT5G06420 | 51.974 | 152 | 23 | 2 | 11 | 112 | 129 | 280 | 7.91e-46 | 152 |
| MS.gene22975.t1 | AT5G06420 | 51.974 | 152 | 23 | 2 | 11 | 112 | 129 | 280 | 7.91e-46 | 152 |
| MS.gene22975.t1 | AT1G01350 | 51.316 | 152 | 24 | 2 | 11 | 112 | 94 | 245 | 1.16e-44 | 149 |
Find 27 sgRNAs with CRISPR-Local
Find 33 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTTCAGACAAACCTTTCT+CGG | 0.310479 | 7.2:-6390411 | None:intergenic |
| TGAAGACTCAAAATGAAAGA+TGG | 0.383661 | 7.2:-6390120 | None:intergenic |
| TTGAAGTCTTTGCATATGTC+AGG | 0.387439 | 7.2:-6390290 | None:intergenic |
| TGACATATGCAAAGACTTCA+AGG | 0.411330 | 7.2:+6390292 | MS.gene22975:CDS |
| AAAATGAAAGATGGGCTTCT+CGG | 0.426527 | 7.2:-6390111 | None:intergenic |
| TGCAAAGACTTCAAGGAGAC+TGG | 0.432731 | 7.2:+6390299 | MS.gene22975:CDS |
| TAGCAAAGCAACAGCAACTT+TGG | 0.436558 | 7.2:+6390166 | MS.gene22975:CDS |
| GAAGACTCAAAATGAAAGAT+GGG | 0.465277 | 7.2:-6390119 | None:intergenic |
| GACCGAGGCAACTACAAGTC+CGG | 0.465608 | 7.2:+6390359 | MS.gene22975:CDS |
| GGTGGCAGTTGGACAAAGAA+TGG | 0.496757 | 7.2:+6390381 | MS.gene22975:CDS |
| GAATGGAATGAAGCCGAGAA+AGG | 0.514321 | 7.2:+6390398 | MS.gene22975:CDS |
| ACCCGGACTTGTAGTTGCCT+CGG | 0.520056 | 7.2:-6390361 | None:intergenic |
| ACCGAGGCAACTACAAGTCC+GGG | 0.533404 | 7.2:+6390360 | MS.gene22975:CDS |
| GAGACTGGGTATTGTGGCTA+CGG | 0.541621 | 7.2:+6390314 | MS.gene22975:CDS |
| TTCTTTGTCCAACTGCCACC+CGG | 0.548782 | 7.2:-6390378 | None:intergenic |
| TTCAAGGAGACTGGGTATTG+TGG | 0.553969 | 7.2:+6390308 | MS.gene22975:CDS |
| GCCGAGAAAGGTTTGTCTGA+AGG | 0.567344 | 7.2:+6390410 | MS.gene22975:CDS |
| GAAACTCTAATATGAGTGGA+AGG | 0.568228 | 7.2:-6390248 | None:intergenic |
| CTACAAGTCCGGGTGGCAGT+TGG | 0.571560 | 7.2:+6390370 | MS.gene22975:CDS |
| TGATGAAACTCTAATATGAG+TGG | 0.597794 | 7.2:-6390252 | None:intergenic |
| ATCCGTGGGTCACACGCACA+CGG | 0.600283 | 7.2:+6390215 | MS.gene22975:CDS |
| GACCGTGTGCGTGTGACCCA+CGG | 0.606621 | 7.2:-6390217 | None:intergenic |
| TGCAAGTTTCTTCACGACCG+AGG | 0.609377 | 7.2:+6390344 | MS.gene22975:CDS |
| GCAAAGACTTCAAGGAGACT+GGG | 0.620309 | 7.2:+6390300 | MS.gene22975:CDS |
| GTGTGCGTGTGACCCACGGA+TGG | 0.641291 | 7.2:-6390213 | None:intergenic |
| ATATGAGTGGAAGGTCTGAG+AGG | 0.681551 | 7.2:-6390239 | None:intergenic |
| GAGGCAACTACAAGTCCGGG+TGG | 0.723997 | 7.2:+6390363 | MS.gene22975:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTTGTCTGAAGGAAAAAAAA+GGG | + | chr7.2:6390421-6390440 | MS.gene22975:CDS | 25.0% |
| GAAGACTCAAAATGAAAGAT+GGG | - | chr7.2:6390122-6390141 | None:intergenic | 30.0% | |
| GTTTGTCTGAAGGAAAAAAA+AGG | + | chr7.2:6390420-6390439 | MS.gene22975:CDS | 30.0% | |
| TGAAGACTCAAAATGAAAGA+TGG | - | chr7.2:6390123-6390142 | None:intergenic | 30.0% | |
| TGAAGGAAAAAAAAGGGAAA+AGG | + | chr7.2:6390427-6390446 | MS.gene22975:CDS | 30.0% | |
| TGATGAAACTCTAATATGAG+TGG | - | chr7.2:6390255-6390274 | None:intergenic | 30.0% | |
| AAAAAGGGAAAAGGACTTTC+TGG | + | chr7.2:6390436-6390455 | MS.gene22975:CDS | 35.0% | |
| GAAACTCTAATATGAGTGGA+AGG | - | chr7.2:6390251-6390270 | None:intergenic | 35.0% | |
| TGACATATGCAAAGACTTCA+AGG | + | chr7.2:6390292-6390311 | MS.gene22975:CDS | 35.0% | |
| TTGAAGTCTTTGCATATGTC+AGG | - | chr7.2:6390293-6390312 | None:intergenic | 35.0% | |
| ! | AAAATGAAAGATGGGCTTCT+CGG | - | chr7.2:6390114-6390133 | None:intergenic | 35.0% |
| !!! | ATTTTTCAAGTTCCATCCGT+GGG | + | chr7.2:6390201-6390220 | MS.gene22975:CDS | 35.0% |
| TAGCAAAGCAACAGCAACTT+TGG | + | chr7.2:6390166-6390185 | MS.gene22975:CDS | 40.0% | |
| TCCTTCAGACAAACCTTTCT+CGG | - | chr7.2:6390414-6390433 | None:intergenic | 40.0% | |
| !!! | CATTTTTCAAGTTCCATCCG+TGG | + | chr7.2:6390200-6390219 | MS.gene22975:CDS | 40.0% |
| ATATGAGTGGAAGGTCTGAG+AGG | - | chr7.2:6390242-6390261 | None:intergenic | 45.0% | |
| GAATGGAATGAAGCCGAGAA+AGG | + | chr7.2:6390398-6390417 | MS.gene22975:CDS | 45.0% | |
| GCAAAGACTTCAAGGAGACT+GGG | + | chr7.2:6390300-6390319 | MS.gene22975:CDS | 45.0% | |
| TGCAAAGACTTCAAGGAGAC+TGG | + | chr7.2:6390299-6390318 | MS.gene22975:CDS | 45.0% | |
| ! | TTCAAGGAGACTGGGTATTG+TGG | + | chr7.2:6390308-6390327 | MS.gene22975:CDS | 45.0% |
| GCCGAGAAAGGTTTGTCTGA+AGG | + | chr7.2:6390410-6390429 | MS.gene22975:CDS | 50.0% | |
| GGTGGCAGTTGGACAAAGAA+TGG | + | chr7.2:6390381-6390400 | MS.gene22975:CDS | 50.0% | |
| TGCAAGTTTCTTCACGACCG+AGG | + | chr7.2:6390344-6390363 | MS.gene22975:CDS | 50.0% | |
| TTCTTTGTCCAACTGCCACC+CGG | - | chr7.2:6390381-6390400 | None:intergenic | 50.0% | |
| ! | GAGACTGGGTATTGTGGCTA+CGG | + | chr7.2:6390314-6390333 | MS.gene22975:CDS | 50.0% |
| ACCCGGACTTGTAGTTGCCT+CGG | - | chr7.2:6390364-6390383 | None:intergenic | 55.0% | |
| ACCGAGGCAACTACAAGTCC+GGG | + | chr7.2:6390360-6390379 | MS.gene22975:CDS | 55.0% | |
| GACCGAGGCAACTACAAGTC+CGG | + | chr7.2:6390359-6390378 | MS.gene22975:CDS | 55.0% | |
| ATCCGTGGGTCACACGCACA+CGG | + | chr7.2:6390215-6390234 | MS.gene22975:CDS | 60.0% | |
| CTACAAGTCCGGGTGGCAGT+TGG | + | chr7.2:6390370-6390389 | MS.gene22975:CDS | 60.0% | |
| GAGGCAACTACAAGTCCGGG+TGG | + | chr7.2:6390363-6390382 | MS.gene22975:CDS | 60.0% | |
| GACCGTGTGCGTGTGACCCA+CGG | - | chr7.2:6390220-6390239 | None:intergenic | 65.0% | |
| GTGTGCGTGTGACCCACGGA+TGG | - | chr7.2:6390216-6390235 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 6390083 | 6390481 | 6390083 | ID=MS.gene22975 |
| chr7.2 | mRNA | 6390083 | 6390481 | 6390083 | ID=MS.gene22975.t1;Parent=MS.gene22975 |
| chr7.2 | exon | 6390083 | 6390481 | 6390083 | ID=MS.gene22975.t1.exon1;Parent=MS.gene22975.t1 |
| chr7.2 | CDS | 6390083 | 6390481 | 6390083 | ID=cds.MS.gene22975.t1;Parent=MS.gene22975.t1 |
| Gene Sequence |
| Protein sequence |
