AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene23199

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23199.t1 MTR_7g050720 90.335 269 20 1 1 263 25 293 2.71e-180 500
MS.gene23199.t1 MTR_7g050590 61.742 264 93 5 1 262 1 258 2.08e-109 320
MS.gene23199.t1 MTR_3g098790 58.689 305 103 9 1 295 28 319 4.31e-108 318
MS.gene23199.t1 MTR_7g050560 58.779 262 87 5 1 262 28 268 1.61e-101 300
MS.gene23199.t1 MTR_7g050710 57.576 264 104 6 2 263 29 286 2.08e-98 293
MS.gene23199.t1 MTR_7g050580 53.992 263 100 7 1 263 28 269 6.64e-85 258
MS.gene23199.t1 MTR_1g035460 36.071 280 148 7 1 263 1 266 5.81e-45 157
MS.gene23199.t1 MTR_7g050640 77.907 86 19 0 1 86 28 113 2.15e-42 142
MS.gene23199.t1 MTR_1g108780 33.910 289 150 9 2 272 13 278 1.35e-41 150
MS.gene23199.t1 MTR_8g103940 32.734 278 149 8 2 263 55 310 1.71e-35 134
MS.gene23199.t1 MTR_3g061320 29.278 263 156 9 2 263 26 259 4.15e-26 105
MS.gene23199.t1 MTR_3g061330 29.268 246 130 5 1 226 25 246 4.65e-26 103
MS.gene23199.t1 MTR_5g044670 30.556 288 165 10 1 276 31 295 7.09e-26 105
MS.gene23199.t1 MTR_1g035570 29.749 279 164 9 2 272 21 275 1.87e-21 94.0
MS.gene23199.t1 MTR_1g035640 32.342 269 145 11 2 263 21 259 3.29e-21 94.0
MS.gene23199.t1 MTR_3g061370 27.376 263 166 6 2 263 23 261 1.68e-20 89.7
MS.gene23199.t1 MTR_3g061290 48.193 83 43 0 2 84 26 108 1.93e-20 87.8
MS.gene23199.t1 MTR_3g061350 25.746 268 164 6 2 263 26 264 5.41e-20 88.2
MS.gene23199.t1 MTR_3g061340 26.103 272 148 7 2 263 26 254 8.11e-20 87.8
MS.gene23199.t1 MTR_3g061310 26.374 273 153 8 2 261 26 263 1.16e-19 87.4
MS.gene23199.t1 MTR_6g043890 25.559 313 214 9 2 307 31 331 6.82e-19 86.3
MS.gene23199.t1 MTR_4g068320 46.067 89 46 1 2 88 22 110 1.07e-18 86.3
MS.gene23199.t1 MTR_1g034240 40.909 110 62 2 2 110 23 130 3.86e-18 84.7
MS.gene23199.t1 MTR_4g119990 27.778 270 177 6 2 263 31 290 2.39e-17 82.0
MS.gene23199.t1 MTR_1g050845 28.137 263 147 7 2 263 25 246 6.06e-17 82.0
MS.gene23199.t1 MTR_5g070440 26.236 263 141 9 2 263 37 247 7.94e-17 79.3
MS.gene23199.t1 MTR_6g061070 29.032 279 172 11 1 263 33 301 1.33e-16 80.5
MS.gene23199.t1 MTR_3g005495 38.372 86 53 0 2 87 29 114 1.52e-16 80.1
MS.gene23199.t1 MTR_3g005495 31.008 129 78 1 2 130 259 376 1.03e-14 74.7
MS.gene23199.t1 MTR_1g035620 28.571 280 164 10 2 277 25 272 4.36e-16 78.6
MS.gene23199.t1 MTR_1g021360 26.471 272 170 6 2 263 198 449 4.43e-16 79.0
MS.gene23199.t1 MTR_1g021400 23.630 292 187 6 2 263 46 331 5.37e-16 78.2
MS.gene23199.t1 MTR_3g005480 37.209 86 54 0 2 87 37 122 6.12e-16 76.6
MS.gene23199.t1 MTR_3g005460 38.372 86 53 0 2 87 41 126 8.76e-16 78.2
MS.gene23199.t1 MTR_3g005460 31.148 122 83 1 2 122 278 399 6.93e-14 72.4
MS.gene23199.t1 MTR_1g035480 35.714 126 73 3 1 121 32 154 1.33e-15 73.9
MS.gene23199.t1 MTR_1g021435 25.559 313 167 10 2 263 38 335 1.47e-15 76.6
MS.gene23199.t1 MTR_3g005440 34.906 106 66 1 2 107 36 138 2.00e-15 76.6
MS.gene23199.t1 MTR_3g005440 34.259 108 71 0 2 109 258 365 6.37e-12 66.2
MS.gene23199.t1 MTR_3g005490 37.209 86 54 0 2 87 37 122 2.31e-15 75.1
MS.gene23199.t1 MTR_1g021270 25.163 306 179 9 2 263 31 330 3.11e-15 76.6
MS.gene23199.t1 MTR_1g033740 37.383 107 64 2 1 107 37 140 6.26e-15 72.4
MS.gene23199.t1 MTR_1g021410 22.772 303 177 6 2 263 38 324 9.95e-15 74.3
MS.gene23199.t1 MTR_3g061220 40.964 83 36 1 2 84 26 95 2.94e-14 68.6
MS.gene23199.t1 MTR_1g034210 43.038 79 45 0 2 80 23 101 5.26e-14 72.0
MS.gene23199.t1 MTR_1g034210 43.038 79 45 0 2 80 23 101 6.82e-14 72.0
MS.gene23199.t1 MTR_1g035510 38.318 107 63 2 1 107 1 104 7.59e-14 70.1
MS.gene23199.t1 MTR_3g005420 30.827 133 92 0 2 134 305 437 2.13e-13 70.9
MS.gene23199.t1 MTR_1g035520 44.318 88 46 2 1 88 87 171 2.79e-13 70.1
MS.gene23199.t1 MTR_1g021320 30.539 167 95 4 2 167 198 344 4.66e-13 70.1
MS.gene23199.t1 MTR_1g021320 40.541 74 44 0 2 75 41 114 8.56e-11 63.2
MS.gene23199.t1 MTR_1g035470 40.625 96 54 2 1 96 1 93 5.56e-13 65.9
MS.gene23199.t1 MTR_1g035650 32.812 192 107 7 2 190 25 197 1.06e-12 66.2
MS.gene23199.t1 MTR_1g021440 41.333 75 44 0 2 76 38 112 3.53e-12 66.2
MS.gene23199.t1 MTR_1g021440 41.333 75 44 0 2 76 38 112 4.12e-12 66.2
MS.gene23199.t1 MTR_1g021440 41.333 75 44 0 2 76 38 112 6.31e-12 66.2
MS.gene23199.t1 MTR_1g021440 41.333 75 44 0 2 76 38 112 6.54e-12 65.9
MS.gene23199.t1 MTR_1g035500 34.579 107 67 2 1 107 40 143 6.67e-12 63.9
MS.gene23199.t1 MTR_1g021290 29.464 112 76 1 2 110 171 282 3.35e-11 63.5
MS.gene23199.t1 MTR_1g021350 36.709 79 50 0 2 80 37 115 9.35e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene23199.t1 AT3G18990 30.968 310 150 11 2 263 22 315 2.32e-28 112
MS.gene23199.t1 AT3G18990 30.323 310 152 10 2 263 22 315 4.49e-28 112
MS.gene23199.t1 AT4G01580 38.095 126 74 2 2 127 46 167 3.05e-19 84.3
MS.gene23199.t1 AT3G18960 44.318 88 49 0 2 89 9 96 4.70e-19 83.2
MS.gene23199.t1 AT3G18960 44.318 88 49 0 2 89 46 133 6.85e-19 83.6
MS.gene23199.t1 AT3G18960 44.318 88 49 0 2 89 46 133 7.12e-19 83.6
MS.gene23199.t1 AT1G49475 41.304 92 53 1 2 93 50 140 1.79e-18 82.0
MS.gene23199.t1 AT4G33280 24.815 270 185 8 1 263 38 296 8.02e-14 71.6

Find 64 sgRNAs with CRISPR-Local

Find 157 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGAAGATGTGCACATGAAAA+TGG 0.182439 7.2:-52064717 None:intergenic
TTTGATCAACCTTGTGAAAT+TGG 0.182486 7.2:+52064886 MS.gene23199:CDS
CTGTGTTAATGTTGGGAAAT+TGG 0.232090 7.2:+52064918 MS.gene23199:CDS
ATTCATCTTAGGTTGTTAAA+TGG 0.238573 7.2:+52066081 MS.gene23199:CDS
TATTTCTCCACAAACTTTCT+TGG 0.252264 7.2:-52064520 None:intergenic
TTTGGTAAAAGACACTTATT+AGG 0.278617 7.2:+52066036 MS.gene23199:CDS
AGAAAGTTTGTGGAGAAATA+TGG 0.293086 7.2:+52064523 MS.gene23199:CDS
ATGATCATACCTTGAAATAT+TGG 0.299709 7.2:-52066317 None:intergenic
CGGTTGAAGGATGCTTCCCC+TGG 0.315737 7.2:+52066761 MS.gene23199:CDS
TAGATCGTTGGATACCCTTT+TGG 0.321526 7.2:-52064785 None:intergenic
GGTTGTTAAATGGGAGAGTT+TGG 0.343233 7.2:+52066091 MS.gene23199:CDS
AAAAGACACTTATTAGGTTT+AGG 0.347950 7.2:+52066042 MS.gene23199:CDS
TTCCTAATTAGATACCTTGC+AGG 0.351726 7.2:-52066114 None:intergenic
GGCCTGCAAGGTATCTAATT+AGG 0.357731 7.2:+52066112 MS.gene23199:CDS
ACCTCCGAATGATGAAGATT+TGG 0.360032 7.2:+52064816 MS.gene23199:CDS
AGCAAGGAGATATTCATCTT+AGG 0.368678 7.2:+52066070 MS.gene23199:CDS
ATTTCTCCACAAACTTTCTT+GGG 0.389419 7.2:-52064519 None:intergenic
TGAATTTGTCCAATATTTCA+AGG 0.405461 7.2:+52066308 MS.gene23199:CDS
CAGTGTATACCAAGTGAGTT+TGG 0.405719 7.2:+52066018 MS.gene23199:intron
CATTGATGCTGCTTGTAATC+TGG 0.414628 7.2:+52065292 MS.gene23199:CDS
TTCATCTTAGGTTGTTAAAT+GGG 0.415306 7.2:+52066082 MS.gene23199:CDS
TGCAAGGTGGAGATTAGATT+TGG 0.421430 7.2:+52064588 MS.gene23199:CDS
TGGACGCATGACAGCAATAA+AGG 0.428279 7.2:-52065264 None:intergenic
TCCAAAGAGTTGAAGCCAAA+AGG 0.428396 7.2:+52064770 MS.gene23199:CDS
TTGAAATATACTCAGGTGAA+TGG 0.430684 7.2:+52066160 MS.gene23199:CDS
AAATATGGTTTCAAAAGGGT+TGG 0.440218 7.2:+52064626 MS.gene23199:CDS
TCTTCCAAATCTTCATCATT+CGG 0.441815 7.2:-52064820 None:intergenic
AAAAGGGTATCCAACGATCT+AGG 0.443698 7.2:+52064787 MS.gene23199:CDS
GATGATCCCAAGAAAGTTTG+TGG 0.445895 7.2:+52064513 None:intergenic
GCAATAGCTGTGTTAATGTT+GGG 0.448923 7.2:+52064911 MS.gene23199:CDS
CCAAAGAGTTGAAGCCAAAA+GGG 0.465061 7.2:+52064771 MS.gene23199:CDS
TCCTTCAACCGTCTCTGTGT+TGG 0.476983 7.2:-52066749 None:intergenic
TGGCTTCAACTCTTTGGAAA+GGG 0.481840 7.2:-52064765 None:intergenic
ATTAGGAAAGGCAATACAAA+AGG 0.485719 7.2:+52066129 MS.gene23199:CDS
GAAGATGTGCACATGAAAAT+GGG 0.487060 7.2:-52064716 None:intergenic
CATATTTGAAAAGCAGAAGA+TGG 0.487743 7.2:-52064684 None:intergenic
CATAGAGTTTGCTCTGTCAA+AGG 0.505840 7.2:-52065225 None:intergenic
TTGGCTTCAACTCTTTGGAA+AGG 0.513756 7.2:-52064766 None:intergenic
GAAGACAACAACTTGAAAGT+TGG 0.520581 7.2:+52066195 MS.gene23199:CDS
AGGTTTATTGCCTAGATCGT+TGG 0.520889 7.2:-52064797 None:intergenic
AATCTCCACCTTGCACCACT+TGG 0.524704 7.2:-52064580 None:intergenic
CAAGCAGCATCAATGTATGA+TGG 0.531742 7.2:-52065284 None:intergenic
ATGGGAGAGTTTGGCCTGCA+AGG 0.535413 7.2:+52066100 MS.gene23199:CDS
TAATGTTGGGAAATTGGAAA+AGG 0.536762 7.2:+52064924 MS.gene23199:CDS
ACCAACACAGAGACGGTTGA+AGG 0.544377 7.2:+52066748 MS.gene23199:CDS
AAGTATCATTCTTTATCTCA+AGG 0.553311 7.2:+52064661 MS.gene23199:CDS
AGCAATAGCTGTGTTAATGT+TGG 0.556338 7.2:+52064910 MS.gene23199:CDS
GCTAGCCTCCCTCAAAACCC+AGG 0.557149 7.2:-52066779 None:intergenic
CGCATGACAGCAATAAAGGA+TGG 0.564569 7.2:-52065260 None:intergenic
TTCAAGGTAATCTCGGATTG+TGG 0.570082 7.2:+52066821 MS.gene23199:CDS
GGTTGAAGGATGCTTCCCCT+GGG 0.575026 7.2:+52066762 MS.gene23199:CDS
TTTGTGGAGAAATATGGAGA+AGG 0.578281 7.2:+52064529 MS.gene23199:CDS
TCCAAATCTTCATCATTCGG+AGG 0.588522 7.2:-52064817 None:intergenic
CTATTGCTTCCAATTTCACA+AGG 0.596543 7.2:-52064895 None:intergenic
GATTACCTTGAAAAGCCTCT+AGG 0.597456 7.2:-52066810 None:intergenic
GCAAGGTATCTAATTAGGAA+AGG 0.604172 7.2:+52066117 MS.gene23199:CDS
CTAGCCTCCCTCAAAACCCA+GGG 0.607813 7.2:-52066778 None:intergenic
TACCTTGAAAAGCCTCTAGG+AGG 0.615390 7.2:-52066807 None:intergenic
TAAAGACTCCAAGTGGTGCA+AGG 0.618798 7.2:+52064572 MS.gene23199:CDS
AAAGAAAACCAACACAGAGA+CGG 0.637921 7.2:+52066741 MS.gene23199:CDS
ATATATCTAAAGACTCCAAG+TGG 0.664356 7.2:+52064565 MS.gene23199:CDS
AGACTCCAAGTGGTGCAAGG+TGG 0.681088 7.2:+52064575 MS.gene23199:CDS
GGCTAGCACTAACCTCCTAG+AGG 0.719203 7.2:+52066795 MS.gene23199:CDS
TAGCCTCCCTCAAAACCCAG+GGG 0.754802 7.2:-52066777 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATTTATTTGACACATT+AGG - chr7.2:52066370-52066389 None:intergenic 15.0%
!! TATAAAAGAAATAGAAACAT+CGG - chr7.2:52066715-52066734 None:intergenic 15.0%
!!! ATTTCTAATCCTTTTTATTT+TGG + chr7.2:52065690-52065709 MS.gene23199:intron 15.0%
!!! ATTTTTGTTTTGAAGAAAAT+TGG + chr7.2:52065740-52065759 MS.gene23199:intron 15.0%
!!! TTTCTAATCCTTTTTATTTT+GGG + chr7.2:52065691-52065710 MS.gene23199:intron 15.0%
!!! TTTTTTTAGTCAAATATGTT+TGG + chr7.2:52065571-52065590 MS.gene23199:intron 15.0%
!! AGAAAAACAACAAAGTTTAT+AGG - chr7.2:52066670-52066689 None:intergenic 20.0%
!! ATCAATGAAAATTATAGCTT+AGG + chr7.2:52065150-52065169 MS.gene23199:intron 20.0%
!! ATTGGTTAAGTTTCAAATTA+TGG + chr7.2:52065082-52065101 MS.gene23199:intron 20.0%
!! ATTTACTTTCACTTTATGAT+TGG + chr7.2:52065064-52065083 MS.gene23199:intron 20.0%
!! CTAGTAATAATCTAATCATT+TGG - chr7.2:52066565-52066584 None:intergenic 20.0%
!! GGTCTATAAATAATGAAAAA+TGG + chr7.2:52065712-52065731 MS.gene23199:intron 20.0%
!! TCAATGAAAATTATAGCTTA+GGG + chr7.2:52065151-52065170 MS.gene23199:intron 20.0%
!! TTTATAGACCCAAAATAAAA+AGG - chr7.2:52065702-52065721 None:intergenic 20.0%
!! TTTGATCAGAGATATTTATA+GGG - chr7.2:52065598-52065617 None:intergenic 20.0%
!!! AAGGTCATAGATTTTTTTAA+TGG + chr7.2:52065341-52065360 MS.gene23199:intron 20.0%
!!! AATTTGGTAATTTGTTATAG+TGG - chr7.2:52065522-52065541 None:intergenic 20.0%
!!! GAAAAATTTGAAATATACTC+AGG + chr7.2:52066153-52066172 MS.gene23199:CDS 20.0%
!!! TAAAAAGTGATGGAAAAATA+TGG + chr7.2:52064611-52064630 MS.gene23199:CDS 20.0%
!!! TAGATTTTTTTAATGGATGA+AGG + chr7.2:52065348-52065367 MS.gene23199:intron 20.0%
!!! TTAGTCCAAACAATTTTATA+CGG + chr7.2:52065620-52065639 MS.gene23199:intron 20.0%
!!! TTTTGATCAGAGATATTTAT+AGG - chr7.2:52065599-52065618 None:intergenic 20.0%
! AAAAGACACTTATTAGGTTT+AGG + chr7.2:52066042-52066061 MS.gene23199:CDS 25.0%
! AAGTATCATTCTTTATCTCA+AGG + chr7.2:52064661-52064680 MS.gene23199:CDS 25.0%
! AGTATCAGTAAATACATTGT+CGG - chr7.2:52065965-52065984 None:intergenic 25.0%
! ATGATCATACCTTGAAATAT+TGG - chr7.2:52066320-52066339 None:intergenic 25.0%
! CTAGTCTAATAACTAAGAAT+TGG + chr7.2:52066581-52066600 MS.gene23199:intron 25.0%
! GAATGAAAAGCTAATAACAT+AGG - chr7.2:52065041-52065060 None:intergenic 25.0%
! GGAAAAATATGGTTTCAAAA+GGG + chr7.2:52064622-52064641 MS.gene23199:CDS 25.0%
! TAAGTCCGTATAAAATTGTT+TGG - chr7.2:52065628-52065647 None:intergenic 25.0%
! TGAATTTGTCCAATATTTCA+AGG + chr7.2:52066308-52066327 MS.gene23199:CDS 25.0%
! TGGAAAAATATGGTTTCAAA+AGG + chr7.2:52064621-52064640 MS.gene23199:CDS 25.0%
! TTTGGTAAAAGACACTTATT+AGG + chr7.2:52066036-52066055 MS.gene23199:CDS 25.0%
!! AATATTTTACACTGTCAACA+AGG + chr7.2:52065847-52065866 MS.gene23199:intron 25.0%
!! ATTCATCTTAGGTTGTTAAA+TGG + chr7.2:52066081-52066100 MS.gene23199:CDS 25.0%
!! CAAATTTTACCCATTAAGTA+GGG + chr7.2:52065536-52065555 MS.gene23199:intron 25.0%
!! CATATTGACAAAAAGTGTAA+AGG + chr7.2:52064961-52064980 MS.gene23199:CDS 25.0%
!! TTAGATTTGGTAAAAAGTGA+TGG + chr7.2:52064601-52064620 MS.gene23199:CDS 25.0%
!! TTCATCTTAGGTTGTTAAAT+GGG + chr7.2:52066082-52066101 MS.gene23199:CDS 25.0%
!!! ACAACTATTTTGCTTTTCTT+TGG + chr7.2:52066608-52066627 MS.gene23199:intron 25.0%
!!! AGAACATTTTTGAAACTCTT+TGG - chr7.2:52066494-52066513 None:intergenic 25.0%
!!! ATGAAGGTCATTCTTTTTTT+GGG + chr7.2:52065364-52065383 MS.gene23199:intron 25.0%
!!! TTGTTATAGTGGTTTTTCAA+GGG - chr7.2:52065511-52065530 None:intergenic 25.0%
!!! TTTGTTATAGTGGTTTTTCA+AGG - chr7.2:52065512-52065531 None:intergenic 25.0%
AAATATGGTTTCAAAAGGGT+TGG + chr7.2:52064626-52064645 MS.gene23199:CDS 30.0%
AGAAAGTTTGTGGAGAAATA+TGG + chr7.2:52064523-52064542 MS.gene23199:CDS 30.0%
ATACTATTGACCCTACTTAA+TGG - chr7.2:52065549-52065568 None:intergenic 30.0%
ATATATCTAAAGACTCCAAG+TGG + chr7.2:52064565-52064584 MS.gene23199:CDS 30.0%
ATTAGGAAAGGCAATACAAA+AGG + chr7.2:52066129-52066148 MS.gene23199:CDS 30.0%
ATTTCTCCACAAACTTTCTT+GGG - chr7.2:52064522-52064541 None:intergenic 30.0%
CATATTTGAAAAGCAGAAGA+TGG - chr7.2:52064687-52064706 None:intergenic 30.0%
CTTCATTGTTCACATCTTTA+GGG + chr7.2:52066263-52066282 MS.gene23199:intron 30.0%
TAATGTTGGGAAATTGGAAA+AGG + chr7.2:52064924-52064943 MS.gene23199:CDS 30.0%
TACTATTGACCCTACTTAAT+GGG - chr7.2:52065548-52065567 None:intergenic 30.0%
TATTTCTCCACAAACTTTCT+TGG - chr7.2:52064523-52064542 None:intergenic 30.0%
TCTTCCAAATCTTCATCATT+CGG - chr7.2:52064823-52064842 None:intergenic 30.0%
TGCAAAATCAACACACTAAA+AGG + chr7.2:52066402-52066421 MS.gene23199:intron 30.0%
TTGAAATATACTCAGGTGAA+TGG + chr7.2:52066160-52066179 MS.gene23199:CDS 30.0%
TTTGATCAACCTTGTGAAAT+TGG + chr7.2:52064886-52064905 MS.gene23199:CDS 30.0%
! CAAACAAAGGTTTTGCTTAA+AGG + chr7.2:52065322-52065341 MS.gene23199:intron 30.0%
! CAGATTTGTGGTATTTTGTA+AGG + chr7.2:52065651-52065670 MS.gene23199:intron 30.0%
! CCAAATTTTACCCATTAAGT+AGG + chr7.2:52065535-52065554 MS.gene23199:intron 30.0%
! CCTACTTAATGGGTAAAATT+TGG - chr7.2:52065538-52065557 None:intergenic 30.0%
!! ATTTTGGGTGGAACAATTTT+TGG - chr7.2:52065461-52065480 None:intergenic 30.0%
!! CATCTTCTGCTTTTCAAATA+TGG + chr7.2:52064685-52064704 MS.gene23199:CDS 30.0%
!!! GAAAGGGTATTTTATCTCTA+AGG - chr7.2:52064752-52064771 None:intergenic 30.0%
!!! GAGCTATTTTGGAATATAGA+AGG + chr7.2:52066429-52066448 MS.gene23199:intron 30.0%
!!! GATGAAGGTCATTCTTTTTT+TGG + chr7.2:52065363-52065382 MS.gene23199:intron 30.0%
AAAGAAAACCAACACAGAGA+CGG + chr7.2:52066741-52066760 MS.gene23199:CDS 35.0%
AAGGTAAGTATAGTGCTAGT+AGG - chr7.2:52066246-52066265 None:intergenic 35.0%
AATCAACACACTAAAAGGAG+TGG + chr7.2:52066407-52066426 MS.gene23199:intron 35.0%
AGCAAGGAGATATTCATCTT+AGG + chr7.2:52066070-52066089 MS.gene23199:CDS 35.0%
AGCAATAGCTGTGTTAATGT+TGG + chr7.2:52064910-52064929 MS.gene23199:CDS 35.0%
CCTAAAGATGTGAACAATGA+AGG - chr7.2:52066265-52066284 None:intergenic 35.0%
CCTTCATTGTTCACATCTTT+AGG + chr7.2:52066262-52066281 MS.gene23199:intron 35.0%
CTATTGCTTCCAATTTCACA+AGG - chr7.2:52064898-52064917 None:intergenic 35.0%
CTGTGTTAATGTTGGGAAAT+TGG + chr7.2:52064918-52064937 MS.gene23199:CDS 35.0%
GAAGACAACAACTTGAAAGT+TGG + chr7.2:52066195-52066214 MS.gene23199:CDS 35.0%
GAAGATGTGCACATGAAAAT+GGG - chr7.2:52064719-52064738 None:intergenic 35.0%
GCAATAGCTGTGTTAATGTT+GGG + chr7.2:52064911-52064930 MS.gene23199:CDS 35.0%
TTAGGTTTAGGCAAAAAGCA+AGG + chr7.2:52066054-52066073 MS.gene23199:CDS 35.0%
TTCCTAATTAGATACCTTGC+AGG - chr7.2:52066117-52066136 None:intergenic 35.0%
TTGAAACTCTTTGGTTGTGA+TGG - chr7.2:52066485-52066504 None:intergenic 35.0%
TTTGTGGAGAAATATGGAGA+AGG + chr7.2:52064529-52064548 MS.gene23199:CDS 35.0%
! GAATTTTGCAGGAAAGCAAT+TGG + chr7.2:52065181-52065200 MS.gene23199:intron 35.0%
! GCAAGGTATCTAATTAGGAA+AGG + chr7.2:52066117-52066136 MS.gene23199:CDS 35.0%
! GTGGTATTTTGTAAGGAGTA+AGG + chr7.2:52065658-52065677 MS.gene23199:intron 35.0%
! TAAAAGGAGTGGAGCTATTT+TGG + chr7.2:52066418-52066437 MS.gene23199:intron 35.0%
! TGTCTTTTACCAAACTCACT+TGG - chr7.2:52066030-52066049 None:intergenic 35.0%
!! TTTTCAAGGGTGAATGATTC+AGG - chr7.2:52065498-52065517 None:intergenic 35.0%
!!! CTTTTTTTGGGTTAATCCTC+CGG + chr7.2:52065376-52065395 MS.gene23199:intron 35.0%
!!! TAGTGTGTTGATTTTGCACA+AGG - chr7.2:52066400-52066419 None:intergenic 35.0%
ACCTCCGAATGATGAAGATT+TGG + chr7.2:52064816-52064835 MS.gene23199:CDS 40.0%
ACGGACTTAACTCAGATTTG+TGG + chr7.2:52065639-52065658 MS.gene23199:intron 40.0%
AGGTTTATTGCCTAGATCGT+TGG - chr7.2:52064800-52064819 None:intergenic 40.0%
ATCTGGTAAGCTGCAAACAA+AGG + chr7.2:52065309-52065328 MS.gene23199:intron 40.0%
ATTCCATCAAGTGAAGCTCA+GGG - chr7.2:52066641-52066660 None:intergenic 40.0%
CAAGCAGCATCAATGTATGA+TGG - chr7.2:52065287-52065306 None:intergenic 40.0%
CAGTGTATACCAAGTGAGTT+TGG + chr7.2:52066018-52066037 MS.gene23199:intron 40.0%
CCAAAGAGTTGAAGCCAAAA+GGG + chr7.2:52064771-52064790 MS.gene23199:CDS 40.0%
CGAAGATGTGCACATGAAAA+TGG - chr7.2:52064720-52064739 None:intergenic 40.0%
GATTACCTTGAAAAGCCTCT+AGG - chr7.2:52066813-52066832 None:intergenic 40.0%
GCTCAAAATCATGCATGAAC+TGG - chr7.2:52066535-52066554 None:intergenic 40.0%
GGCTAGGTTATACTTCTTCA+CGG - chr7.2:52065440-52065459 None:intergenic 40.0%
GGTTGTTAAATGGGAGAGTT+TGG + chr7.2:52066091-52066110 MS.gene23199:CDS 40.0%
TCCAAAGAGTTGAAGCCAAA+AGG + chr7.2:52064770-52064789 MS.gene23199:CDS 40.0%
TCCAAATCTTCATCATTCGG+AGG - chr7.2:52064820-52064839 None:intergenic 40.0%
TGCAAGGTGGAGATTAGATT+TGG + chr7.2:52064588-52064607 MS.gene23199:CDS 40.0%
TTCAAGGTAATCTCGGATTG+TGG + chr7.2:52066821-52066840 MS.gene23199:CDS 40.0%
! CATAGAGTTTGCTCTGTCAA+AGG - chr7.2:52065228-52065247 None:intergenic 40.0%
! CATTGATGCTGCTTGTAATC+TGG + chr7.2:52065292-52065311 MS.gene23199:CDS 40.0%
! CTTTGGTTGTGATGGTGATT+TGG - chr7.2:52066477-52066496 None:intergenic 40.0%
! TAGATCGTTGGATACCCTTT+TGG - chr7.2:52064788-52064807 None:intergenic 40.0%
! TGGCTTCAACTCTTTGGAAA+GGG - chr7.2:52064768-52064787 None:intergenic 40.0%
! TTGGCTTCAACTCTTTGGAA+AGG - chr7.2:52064769-52064788 None:intergenic 40.0%
!! AAAAGGGTATCCAACGATCT+AGG + chr7.2:52064787-52064806 MS.gene23199:CDS 40.0%
!! GAGGCTTTTCAAGGTAATCT+CGG + chr7.2:52066814-52066833 MS.gene23199:CDS 40.0%
CATTCCATCAAGTGAAGCTC+AGG - chr7.2:52066642-52066661 None:intergenic 45.0%
CGCATGACAGCAATAAAGGA+TGG - chr7.2:52065263-52065282 None:intergenic 45.0%
CGGCTGAACTTCAGATTATC+AGG - chr7.2:52065420-52065439 None:intergenic 45.0%
GTTCCACCCAAAATCGAACT+CGG + chr7.2:52065467-52065486 MS.gene23199:intron 45.0%
TAAAGACTCCAAGTGGTGCA+AGG + chr7.2:52064572-52064591 MS.gene23199:CDS 45.0%
TACCTTGAAAAGCCTCTAGG+AGG - chr7.2:52066810-52066829 None:intergenic 45.0%
TGGACGCATGACAGCAATAA+AGG - chr7.2:52065267-52065286 None:intergenic 45.0%
TTCCACCCAAAATCGAACTC+GGG + chr7.2:52065468-52065487 MS.gene23199:intron 45.0%
TTCCATCAAGTGAAGCTCAG+GGG - chr7.2:52066640-52066659 None:intergenic 45.0%
! AACCTCCTAGAGGCTTTTCA+AGG + chr7.2:52066805-52066824 MS.gene23199:CDS 45.0%
! AGGGTGTGCTTGAATTTTGC+AGG + chr7.2:52065170-52065189 MS.gene23199:intron 45.0%
! GAGAACCCGAGTTCGATTTT+GGG - chr7.2:52065476-52065495 None:intergenic 45.0%
! GGCCTGCAAGGTATCTAATT+AGG + chr7.2:52066112-52066131 MS.gene23199:CDS 45.0%
!!! CCCTTTTGGCTTCAACTCTT+TGG - chr7.2:52064774-52064793 None:intergenic 45.0%
!!! GGGTGGAACAATTTTTGGCT+AGG - chr7.2:52065456-52065475 None:intergenic 45.0%
AATCTCCACCTTGCACCACT+TGG - chr7.2:52064583-52064602 None:intergenic 50.0%
ACCAACACAGAGACGGTTGA+AGG + chr7.2:52066748-52066767 MS.gene23199:CDS 50.0%
GGGTTAATCCTCCGGTTCTT+GGG + chr7.2:52065384-52065403 MS.gene23199:intron 50.0%
TCCTTCAACCGTCTCTGTGT+TGG - chr7.2:52066752-52066771 None:intergenic 50.0%
TGGGTTAATCCTCCGGTTCT+TGG + chr7.2:52065383-52065402 MS.gene23199:intron 50.0%
! AACCCGAGTTCGATTTTGGG+TGG - chr7.2:52065473-52065492 None:intergenic 50.0%
! GGAGAACCCGAGTTCGATTT+TGG - chr7.2:52065477-52065496 None:intergenic 50.0%
!! ATGCTTCCCCTGGGTTTTGA+GGG + chr7.2:52066771-52066790 MS.gene23199:CDS 50.0%
AGACTCCAAGTGGTGCAAGG+TGG + chr7.2:52064575-52064594 MS.gene23199:CDS 55.0%
ATCCTCCGGTTCTTGGGAGA+AGG + chr7.2:52065390-52065409 MS.gene23199:intron 55.0%
ATGGGAGAGTTTGGCCTGCA+AGG + chr7.2:52066100-52066119 MS.gene23199:CDS 55.0%
CTAGCCTCCCTCAAAACCCA+GGG - chr7.2:52066781-52066800 None:intergenic 55.0%
GGCTAGCACTAACCTCCTAG+AGG + chr7.2:52066795-52066814 MS.gene23199:CDS 55.0%
GGTTGAAGGATGCTTCCCCT+GGG + chr7.2:52066762-52066781 MS.gene23199:CDS 55.0%
TAGCCTCCCTCAAAACCCAG+GGG - chr7.2:52066780-52066799 None:intergenic 55.0%
TCCTCCGGTTCTTGGGAGAA+GGG + chr7.2:52065391-52065410 MS.gene23199:intron 55.0%
TGCCCCTGAGCTTCACTTGA+TGG + chr7.2:52066635-52066654 MS.gene23199:intron 55.0%
! GATGCTTCCCCTGGGTTTTG+AGG + chr7.2:52066770-52066789 MS.gene23199:CDS 55.0%
CCTCCGGTTCTTGGGAGAAG+GGG + chr7.2:52065392-52065411 MS.gene23199:intron 60.0%
CGGTTGAAGGATGCTTCCCC+TGG + chr7.2:52066761-52066780 MS.gene23199:CDS 60.0%
CTCCGGTTCTTGGGAGAAGG+GGG + chr7.2:52065393-52065412 MS.gene23199:intron 60.0%
GACCCCCTTCTCCCAAGAAC+CGG - chr7.2:52065398-52065417 None:intergenic 60.0%
! GCTAGCCTCCCTCAAAACCC+AGG - chr7.2:52066782-52066801 None:intergenic 60.0%
!!! CTTCCCCTGGGTTTTGAGGG+AGG + chr7.2:52066774-52066793 MS.gene23199:CDS 60.0%
CCCCTTCTCCCAAGAACCGG+AGG - chr7.2:52065395-52065414 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 52064514 52066846 52064514 ID=MS.gene23199
chr7.2 mRNA 52064514 52066846 52064514 ID=MS.gene23199.t1;Parent=MS.gene23199
chr7.2 exon 52064514 52064982 52064514 ID=MS.gene23199.t1.exon1;Parent=MS.gene23199.t1
chr7.2 CDS 52064514 52064982 52064514 ID=cds.MS.gene23199.t1;Parent=MS.gene23199.t1
chr7.2 exon 52065192 52065313 52065192 ID=MS.gene23199.t1.exon2;Parent=MS.gene23199.t1
chr7.2 CDS 52065192 52065313 52065192 ID=cds.MS.gene23199.t1;Parent=MS.gene23199.t1
chr7.2 exon 52066021 52066216 52066021 ID=MS.gene23199.t1.exon3;Parent=MS.gene23199.t1
chr7.2 CDS 52066021 52066216 52066021 ID=cds.MS.gene23199.t1;Parent=MS.gene23199.t1
chr7.2 exon 52066284 52066329 52066284 ID=MS.gene23199.t1.exon4;Parent=MS.gene23199.t1
chr7.2 CDS 52066284 52066329 52066284 ID=cds.MS.gene23199.t1;Parent=MS.gene23199.t1
chr7.2 exon 52066738 52066846 52066738 ID=MS.gene23199.t1.exon5;Parent=MS.gene23199.t1
chr7.2 CDS 52066738 52066846 52066738 ID=cds.MS.gene23199.t1;Parent=MS.gene23199.t1
Gene Sequence

>MS.gene23199

ATGATCCCAAGAAAGTTTGTGGAGAAATATGGAGAAGGTTTATCAAATGCTATATATCTAAAGACTCCAAGTGGTGCAAGGTGGAGATTAGATTTGGTAAAAAGTGATGGAAAAATATGGTTTCAAAAGGGTTGGAAAGAATTTGTGAAGTATCATTCTTTATCTCAAGGCCATCTTCTGCTTTTCAAATATGGAAGAACTTCCCATTTTCATGTGCACATCTTCGATAAGAGTGCCTTAGAGATAAAATACCCTTTCCAAAGAGTTGAAGCCAAAAGGGTATCCAACGATCTAGGCAATAAACCTCCGAATGATGAAGATTTGGAAGATTATATAGCAAGTAAAAAGAGAAAAACTAACTTGTCATTAGAATTTGATCAACCTTGTGAAATTGGAAGCAATAGCTGTGTTAATGTTGGGAAATTGGAAAAGGTAGCAGCTTTGAATCATATTGACAAAAAGTGTAAAGGTATAAGAATTTGTGTTGTGAATGTCTCATGAATCATGCATACTTCACATGTTAGCCTATGTTATTAGCTTTTCATTCTTAATTTACTTTCACTTTATGATTGGTTAAGTTTCAAATTATGGCATGCAAGTTGCAACTTGTTCATTATATTTCAATTTTTATAATAGATCAATGAAAATTATAGCTTAGGGTGTGCTTGAATTTTGCAGGAAAGCAATTGGCTGTTGCTAAGAAAGTTACAACCTTTGACAGAGCAAACTCTATGAGAACTTGTAATCCATCCTTTATTGCTGTCATGCGTCCATCATACATTGATGCTGCTTGTAATCTGGTAAGCTGCAAACAAAGGTTTTGCTTAAAGGTCATAGATTTTTTTAATGGATGAAGGTCATTCTTTTTTTGGGTTAATCCTCCGGTTCTTGGGAGAAGGGGGTCCTGATAATCTGAAGTTCAGCCGTGAAGAAGTATAACCTAGCCAAAAATTGTTCCACCCAAAATCGAACTCGGGTTCTCCTGAATCATTCACCCTTGAAAAACCACTATAACAAATTACCAAATTTTACCCATTAAGTAGGGTCAATAGTATTTTTTTTTTAGTCAAATATGTTTGGTCCCTATAAATATCTCTGATCAAAATTTAGTCCAAACAATTTTATACGGACTTAACTCAGATTTGTGGTATTTTGTAAGGAGTAAGGACTACAAACATTTCTAATCCTTTTTATTTTGGGTCTATAAATAATGAAAAATGGAAACTATTTTTGTTTTGAAGAAAATTGGAAACTACTTATTGAAAACTTCAATGTTAATCTTTATTGATAATTTGTAAAGAGTTGATATGTTGCTATATTGTCATCAGTTTAAAATATTTTACACTGTCAACAAGGCAGTCAATCATAGATGTGACTTTTGAAGTAGTTATGCTTAAAGTCAAATATTTTTACACTGACAATGTATTTCAAAAAACTATACTTTTACACCGACAATGTATTTACTGATACTCAAGAACATTTGTTTTTTTTTTCTTTTGCTTGACAGTGTATACCAAGTGAGTTTGGTAAAAGACACTTATTAGGTTTAGGCAAAAAGCAAGGAGATATTCATCTTAGGTTGTTAAATGGGAGAGTTTGGCCTGCAAGGTATCTAATTAGGAAAGGCAATACAAAAGGAGAAAAATTTGAAATATACTCAGGTGAATGGAAGACATTTGCAGAAGACAACAACTTGAAAGTTGGTGATGTTTGCACTTTTGAGCTCTTTCCTACTAGCACTATACTTACCTTCATTGTTCACATCTTTAGGGATTCTTTTGAGACATAAACAATTGAATTTGTCCAATATTTCAAGGTATGATCATGTTTAATTTTGTTGTTTGAAAACTTTTCCTAATGTGTCAAATAAATTTATTTATCTACCTTGTGCAAAATCAACACACTAAAAGGAGTGGAGCTATTTTGGAATATAGAAGGAACATGAAAATCATCTTTACTACCAAATCACCATCACAACCAAAGAGTTTCAAAAATGTTCTATGCGCTCAATGTTTGATCCAGTTCATGCATGATTTTGAGCACTACTACCAAATGATTAGATTATTACTAGTCTAATAACTAAGAATTGGTACAACAACTATTTTGCTTTTCTTTGGTGTTTGCCCCTGAGCTTCACTTGATGGAATGTAATTCCTATAAACTTTGTTGTTTTTCTTCATGAATTTTTGTATTTGTCTCCGATGTTTCTATTTCTTTTATACAGAAAAAAGAAAACCAACACAGAGACGGTTGAAGGATGCTTCCCCTGGGTTTTGAGGGAGGCTAGCACTAACCTCCTAGAGGCTTTTCAAGGTAATCTCGGATTGTGGTGA
Protein sequence

>MS.gene23199.t1

MIPRKFVEKYGEGLSNAIYLKTPSGARWRLDLVKSDGKIWFQKGWKEFVKYHSLSQGHLLLFKYGRTSHFHVHIFDKSALEIKYPFQRVEAKRVSNDLGNKPPNDEDLEDYIASKKRKTNLSLEFDQPCEIGSNSCVNVGKLEKVAALNHIDKKCKGKQLAVAKKVTTFDRANSMRTCNPSFIAVMRPSYIDAACNLCIPSEFGKRHLLGLGKKQGDIHLRLLNGRVWPARYLIRKGNTKGEKFEIYSGEWKTFAEDNNLKVGILLRHKQLNLSNISRKKKTNTETVEGCFPWVLREASTNLLEAFQGNLGLW