Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23228.t1 | XP_024626636.1 | 72.1 | 111 | 30 | 1 | 1 | 111 | 178 | 287 | 9.90E-38 | 166 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23228.t1 | Q6AV21 | 49.1 | 57 | 28 | 1 | 29 | 84 | 469 | 525 | 2.7e-07 | 56.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23228.t1 | G7KVV6 | 72.1 | 111 | 30 | 1 | 1 | 111 | 206 | 315 | 7.1e-38 | 166.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene23228.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene23228.t1 | MTR_7g050580 | 72.072 | 111 | 30 | 1 | 1 | 111 | 206 | 315 | 5.20e-53 | 168 |
| MS.gene23228.t1 | MTR_3g098790 | 75.758 | 99 | 23 | 1 | 1 | 99 | 215 | 312 | 8.77e-51 | 163 |
| MS.gene23228.t1 | MTR_7g050560 | 66.667 | 111 | 35 | 2 | 1 | 111 | 207 | 315 | 4.08e-46 | 150 |
| MS.gene23228.t1 | MTR_7g050590 | 73.000 | 100 | 25 | 2 | 1 | 99 | 195 | 293 | 7.53e-45 | 147 |
| MS.gene23228.t1 | MTR_7g050710 | 64.356 | 101 | 33 | 2 | 1 | 99 | 221 | 320 | 1.49e-39 | 134 |
| MS.gene23228.t1 | MTR_7g050720 | 59.783 | 92 | 32 | 5 | 1 | 88 | 227 | 317 | 2.57e-26 | 99.8 |
| MS.gene23228.t1 | MTR_1g108780 | 40.698 | 86 | 41 | 2 | 1 | 86 | 215 | 290 | 5.55e-15 | 69.7 |
| MS.gene23228.t1 | MTR_1g035460 | 40.860 | 93 | 44 | 3 | 1 | 93 | 213 | 294 | 1.13e-14 | 68.6 |
| MS.gene23228.t1 | MTR_8g103940 | 43.023 | 86 | 39 | 2 | 1 | 86 | 256 | 331 | 1.12e-13 | 65.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 17 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCCTGCAAGGTACGTAATT+AGG | 0.328975 | 7.2:-52476765 | MS.gene23228:CDS |
| ATCCTAATTACGTACCTTGC+AGG | 0.349025 | 7.2:+52476763 | None:intergenic |
| TGTTAACTAGCGGAAGAGTT+TGG | 0.409297 | 7.2:-52476786 | MS.gene23228:CDS |
| CAGACTATACCGTCTATGTT+TGG | 0.418381 | 7.2:-52476859 | MS.gene23228:CDS |
| TGGTATGAGACACTTCGATT+TGG | 0.435683 | 7.2:-52476839 | MS.gene23228:CDS |
| TTCGATTTGGATAAGAAGAA+AGG | 0.460599 | 7.2:-52476826 | MS.gene23228:CDS |
| AAGGACAATAACTTAAAAGT+TGG | 0.486873 | 7.2:-52476688 | MS.gene23228:CDS |
| ATAAAAGAAGAAGATATTCA+AGG | 0.505867 | 7.2:-52476466 | MS.gene23228:CDS |
| AACACAAATCTTACTTTGAG+AGG | 0.511657 | 7.2:+52476570 | None:intergenic |
| AATTTGAACTCACAGGTGGA+TGG | 0.559842 | 7.2:-52476723 | MS.gene23228:CDS |
| ACATTAAAATTTGAACTCAC+AGG | 0.562239 | 7.2:-52476730 | MS.gene23228:CDS |
| GCGGAAGAGTTTGGCCTGCA+AGG | 0.576423 | 7.2:-52476777 | MS.gene23228:CDS |
| TTAAAATTTGAACTCACAGG+TGG | 0.579026 | 7.2:-52476727 | MS.gene23228:CDS |
| CACTGTTCACATCTTTAGAG+AGG | 0.585792 | 7.2:-52476620 | MS.gene23228:CDS |
| TGAACTCACAGGTGGATGGA+AGG | 0.586048 | 7.2:-52476719 | MS.gene23228:CDS |
| TGGATGGAAGGCATTTGCAA+AGG | 0.640290 | 7.2:-52476707 | MS.gene23228:CDS |
| TGTCTCATACCAAACATAGA+CGG | 0.687526 | 7.2:+52476850 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAAAATATAATTAGATAACT+AGG | - | chr7.2:52476466-52476485 | MS.gene23228:CDS | 10.0% |
| !!! | TTATTTGTTATATGAAAACA+TGG | + | chr7.2:52476889-52476908 | None:intergenic | 15.0% |
| !! | ATAAAAGAAGAAGATATTCA+AGG | - | chr7.2:52476943-52476962 | MS.gene23228:intron | 20.0% |
| !!! | GAATATCTTCTTCTTTTATA+CGG | + | chr7.2:52476942-52476961 | None:intergenic | 20.0% |
| ! | AAGGACAATAACTTAAAAGT+TGG | - | chr7.2:52476721-52476740 | MS.gene23228:CDS | 25.0% |
| ! | ACATTAAAATTTGAACTCAC+AGG | - | chr7.2:52476679-52476698 | MS.gene23228:CDS | 25.0% |
| !!! | GAAAGGAGATATTTATTTTC+AGG | - | chr7.2:52476600-52476619 | MS.gene23228:CDS | 25.0% |
| !!! | TATTTGCTTTTTCTAATGCT+TGG | - | chr7.2:52476527-52476546 | MS.gene23228:intron | 25.0% |
| AACACAAATCTTACTTTGAG+AGG | + | chr7.2:52476842-52476861 | None:intergenic | 30.0% | |
| TTAAAATTTGAACTCACAGG+TGG | - | chr7.2:52476682-52476701 | MS.gene23228:CDS | 30.0% | |
| !! | TTCGATTTGGATAAGAAGAA+AGG | - | chr7.2:52476583-52476602 | MS.gene23228:intron | 30.0% |
| !!! | TATTTTCAGGTGTTAACTAG+CGG | - | chr7.2:52476613-52476632 | MS.gene23228:CDS | 30.0% |
| CGGTAAGATTCTGCATAAAA+TGG | + | chr7.2:52476922-52476941 | None:intergenic | 35.0% | |
| TGTCTCATACCAAACATAGA+CGG | + | chr7.2:52476562-52476581 | None:intergenic | 35.0% | |
| AATTTGAACTCACAGGTGGA+TGG | - | chr7.2:52476686-52476705 | MS.gene23228:CDS | 40.0% | |
| ATCCTAATTACGTACCTTGC+AGG | + | chr7.2:52476649-52476668 | None:intergenic | 40.0% | |
| CAGACTATACCGTCTATGTT+TGG | - | chr7.2:52476550-52476569 | MS.gene23228:intron | 40.0% | |
| TGGTATGAGACACTTCGATT+TGG | - | chr7.2:52476570-52476589 | MS.gene23228:intron | 40.0% | |
| TGTTAACTAGCGGAAGAGTT+TGG | - | chr7.2:52476623-52476642 | MS.gene23228:CDS | 40.0% | |
| ! | CACTGTTCACATCTTTAGAG+AGG | - | chr7.2:52476789-52476808 | MS.gene23228:CDS | 40.0% |
| !! | TGGATGGAAGGCATTTGCAA+AGG | - | chr7.2:52476702-52476721 | MS.gene23228:CDS | 45.0% |
| GGCCTGCAAGGTACGTAATT+AGG | - | chr7.2:52476644-52476663 | MS.gene23228:CDS | 50.0% | |
| TGAACTCACAGGTGGATGGA+AGG | - | chr7.2:52476690-52476709 | MS.gene23228:CDS | 50.0% | |
| GCGGAAGAGTTTGGCCTGCA+AGG | - | chr7.2:52476632-52476651 | MS.gene23228:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 52476462 | 52476969 | 52476462 | ID=MS.gene23228 |
| chr7.2 | mRNA | 52476462 | 52476969 | 52476462 | ID=MS.gene23228.t1;Parent=MS.gene23228 |
| chr7.2 | exon | 52476967 | 52476969 | 52476967 | ID=MS.gene23228.t1.exon1;Parent=MS.gene23228.t1 |
| chr7.2 | CDS | 52476967 | 52476969 | 52476967 | ID=cds.MS.gene23228.t1;Parent=MS.gene23228.t1 |
| chr7.2 | exon | 52476584 | 52476878 | 52476584 | ID=MS.gene23228.t1.exon2;Parent=MS.gene23228.t1 |
| chr7.2 | CDS | 52476584 | 52476878 | 52476584 | ID=cds.MS.gene23228.t1;Parent=MS.gene23228.t1 |
| chr7.2 | exon | 52476462 | 52476499 | 52476462 | ID=MS.gene23228.t1.exon3;Parent=MS.gene23228.t1 |
| chr7.2 | CDS | 52476462 | 52476499 | 52476462 | ID=cds.MS.gene23228.t1;Parent=MS.gene23228.t1 |
| Gene Sequence |
| Protein sequence |
